Artificial cultivation method for increasing flavone content of morchella esculenta

Abstract

The invention discloses an artificial cultivation method for improving the flavone content of morchella. The method comprises the following steps: producing seeds by using a morchella engineering strain; furrows are built according to cultivation technical specifications; sowing is conducted on the built furrows; performing spawn running management according to technical specifications; soaking buckwheat grains in warm water, uniformly mixing the buckwheat grains with crushed ginkgo leaves, wood chips and rice husks, adding water to a specified water content, bagging, performing high-temperature steam sterilization, and cooling for later use; placing exogenous nutrition bags: placing the prepared exogenous nutrition bags; before and after morchella primordium appears, a strigolactones (GR24) aqueous solution is sprayed on the ground according to the spraying amount; and harvesting the morchella according to technical specifications. According to the method disclosed by the invention, the total flavonoid content of the morchella esculenta product is remarkably improved by using the morchella esculenta engineering strain capable of producing flavonoids at high yield and plant hormones capable of promoting the morchella esculenta to produce flavonoids at high yield, and replacing wheat grains in a nutrition bag with buckwheat grains with high flavonoid content, so that the medicinal value of the morchella esculenta product is enhanced.

Description

Technical Field

[0001] The present invention belongs to the technical field of edible mushroom cultivation, and specifically relates to an artificial cultivation method for increasing the flavonoid content in Morchella esculenta. Background Art

[0002] Morchella esculenta is a rare edible mushroom belonging to the Ascomycota, Discomycetes, Morchellales, and Morchellaceae in the fungal kingdom. Morchella esculenta is famous for the unevenness of its cap and the texture similar to that of a sheep's stomach, and its unique appearance and flavor are highly favored by edible mushroom lovers. Morchella esculenta not only has excellent flavor and high nutritional density, but also has various medicinal properties, such as enhancing the body's immunity and reducing the cholesterol level in the body. Flavonoids are a type of natural organic compound widely present in nature and belong to polyphenols. In recent years, due to their diverse biological effects and positive impacts on health, flavonoids have attracted extensive attention, and they have been proven to have various medicinal functions such as enhancing immunity, resisting oxidative stress, and maintaining cardiovascular health. However, under the traditional artificial cultivation mode of Morchella esculenta, the yield of flavonoids is usually low, which limits its extensive use in medical applications. Summary of the Invention

[0003] In view of this, the main purpose of the present invention is to provide an artificial cultivation method for increasing the flavonoid content in Morchella esculenta.

[0004] To achieve the above object, the technical solution of the present invention is realized as follows: The embodiment of the present invention provides an artificial cultivation method for increasing the flavonoid content in Morchella esculenta, comprising the following steps: a) Seed production: Use the engineering strain of Morchella esculenta for seed production; b) Bed building: Build beds according to the cultivation technical specifications; c) Sowing: Sow on the built beds; d) Mycelium management: Conduct mycelium management according to the technical specifications; e) Preparation of exogenous nutrient bags: Soak buckwheat grains in warm water, mix them with crushed ginkgo leaves, sawdust, and rice husks, add water to the specified moisture content, bag them, sterilize them with high-temperature steam, and cool for later use; f) Placement of exogenous nutrient bags: Place the prepared exogenous nutrient bags; g) Mushroom fruiting management: Before and after the appearance of Morchella esculenta primordia, spray the ground with an aqueous solution of strigolactone (GR24) at a spraying amount; h) Harvesting: Harvest Morchella esculenta according to the technical specifications.

[0005] In the above-mentioned solution, the Morchella importuna strain used in the seed production stage is the engineered Morchella importuna strain MM0483, which was deposited at the China Center for Type Culture Collection on November 20, 2024, with the deposit number CCTCC No. M20242606.

[0006] In the above-mentioned solution, the preparation steps of the exogenous nutrient bag specifically include: soaking buckwheat grains in warm water with an initial temperature of 45°C for 24 hours, then mixing them with crushed ginkgo leaves, wood chips, and rice husks in a ratio of 1:2:3:4, adding water to a water content of 55%, then bagging, and sterilizing with high-temperature steam at 121°C for 2 hours, and storing for use after cooling to room temperature.

[0007] In the above-mentioned solution, the mushroom fruiting management steps specifically include: spraying an aqueous solution of strigolactone (GR24) with a concentration of 15 μg / L at a spraying amount of 130 ml / m² on the ground before and after the appearance of Morchella importuna primordia.

[0008] Compared with the prior art, the beneficial effects of the present invention are as follows: By using an engineered Morchella importuna strain with high flavonoid production, as well as a plant hormone that can promote high flavonoid production in Morchella importuna, and by replacing the wheat grains in the nutrient bag with buckwheat grains with high flavonoid content and other means, the total flavonoid content of the Morchella importuna product is significantly increased, thereby enhancing its medicinal value. Detailed implementation mode

[0009] In order to make the objectives, technical solutions, and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention.

[0010] The embodiment of the present invention provides an artificial cultivation method for increasing the flavonoid content of Morchella importuna, including the following steps: a) Seed production: Use an engineered Morchella importuna strain for seed production; Specifically, the Morchella importuna strain used in the seed production stage is the engineered Morchella importuna strain MM0483, which was deposited at the China Center for Type Culture Collection on November 20, 2024, with the deposit number CCTCC No. M20242606.

[0011] b) Bed building: Build beds according to the cultivation technical specifications; Specifically, the bed building work for the artificial cultivation of Morchella importuna is carried out in accordance with the local standard of Shaanxi Province, "Technical Specification for Cultivating Morchella importuna in Greenhouses" (DB61T 1518-2021).

[0012] c) Sowing: Sow on the already built beds; Specifically, the sowing work of artificial cultivation of Morchella esculenta is carried out in accordance with the local standard of Shaanxi Province, "Technical Specification for Cultivation of Morchella esculenta in Greenhouses" (DB61T 1518-2021).

[0013] d) Mycelium growth management: Carry out mycelium growth management in accordance with the technical specifications; Specifically, the mycelium growth management work of artificial cultivation of Morchella esculenta is carried out in accordance with the local standard of Shaanxi Province, "Technical Specification for Cultivation of Morchella esculenta in Greenhouses" (DB61T 1518-2021).

[0014] e) Preparation of exogenous nutrient bags: Soak buckwheat grains in warm water, mix them with crushed ginkgo leaves, sawdust, and rice husks, add water to the specified moisture content, bag them, sterilize them with high-temperature steam, and cool for later use; Specifically, soak buckwheat grains in warm water with an initial temperature of 45 °C for 24 hours, then mix them with crushed ginkgo leaves, sawdust, and rice husks in a ratio of 1:2:3:4, add water to a moisture content of 55%, then bag them, and sterilize them with high-temperature steam at 121 °C for 2 hours. After cooling to room temperature, they are ready for use.

[0015] The crushed ginkgo leaves, sawdust, and rice husks pass through a 50-mesh sieve.

[0016] e) Placement of exogenous nutrient bags: Place the prepared exogenous nutrient bags; Specifically, the placement work of nutrient bags for artificial cultivation of Morchella esculenta is carried out in accordance with the local standard of Shaanxi Province, "Technical Specification for Cultivation of Morchella esculenta in Greenhouses" (DB61T 1518-2021).

[0017] g) Mushroom fruiting management: Before and after the appearance of Morchella esculenta primordia, spray the ground with an aqueous solution of strigolactone (GR24); Specifically, before and after the appearance of Morchella esculenta primordia, spray the ground with an aqueous solution of strigolactone (GR24) with a concentration of 15 μg / L at a spraying rate of 130 ml / m².

[0018] Carry out the mushroom fruiting management work of artificial cultivation of Morchella esculenta in accordance with the local standard of Shaanxi Province, "Technical Specification for Cultivation of Morchella esculenta in Greenhouses" (DB61T 1518-2021). At the same time, before and after the appearance of Morchella esculenta primordia, spray the ground with an aqueous solution of strigolactone (GR24) with a concentration of 15 μg / L at a spraying rate of 130 ml / m 2 Spray amount.

[0019] h) Harvesting: Harvest Morchella esculenta in accordance with the technical specifications.

[0020] Specifically, carry out the harvesting work of artificial cultivation of Morchella esculenta in accordance with the local standard of Shaanxi Province, "Technical Specification for Cultivation of Morchella esculenta in Greenhouses" (DB61T 1518-2021).

[0021] The present invention significantly increases the total flavonoid content of the morel product and thus enhances its medicinal value by using a morel engineering strain with high flavonoid yield, a phytohormone that can promote high flavonoid yield in morel, and by replacing the wheat grains in the nutrient bag with buckwheat grains with high flavonoid content, etc.

[0022] The above is only a preferred embodiment of the present invention and is not intended to limit the protection scope of the present invention.

Claims

1. An artificial cultivation method for increasing the flavonoid content of Morchella esculenta, characterized in that, It includes the following steps: a) Seed production: Use the engineered strain of Morchella to produce seeds; b) Bed construction: Construct beds according to the cultivation technical specifications; c) Sowing: Sow on the constructed beds; d) Mycelium growth management: Manage the mycelium growth according to the technical specifications; e) Preparation of exogenous nutrient bags: Soak buckwheat grains in warm water, mix them with ground ginkgo leaves, sawdust, and chaff, add water to the specified moisture content, bag them, sterilize them with high-temperature steam, and cool them for later use; f) Placement of exogenous nutrient bags: Place the prepared exogenous nutrient bags; g) Mushroom fruiting management: Before and after the appearance of Morchella primordia, spray the ground with an aqueous solution of strigolactone (GR24) at a spraying rate of 130 ml / m²; h) Harvesting: Harvest Morchella according to the technical specifications.

2. The artificial cultivation method for increasing the flavonoid content of Morchella esculenta according to claim 1, characterized in that The Morchella strain used in the seed production stage is the engineered strain Morchella importuna MM0483, which was deposited at the China Center for Type Culture Collection on November 20, 2024, with the deposit number CCTCC No. M20242606.

3. The artificial cultivation method for increasing the flavonoid content of Morchella esculenta according to claim 1 or 2, characterized in that, The specific steps for preparing the exogenous nutrient bags include: Soak buckwheat grains in warm water with an initial temperature of 45°C for 24 hours, then mix them with ground ginkgo leaves, sawdust, and chaff in a ratio of 1:2:3:4, add water to a moisture content of 55%, then bag them, and sterilize them with high-temperature steam at 121°C for 2 hours, and cool them to room temperature for later use.

4. The artificial cultivation method for increasing the flavonoid content of Morchella esculenta according to claim 3, wherein The specific steps for mushroom fruiting management include: Before and after the appearance of Morchella primordia, spray the ground with an aqueous solution of strigolactone (GR24) at a concentration of 15 ug / L at a spraying rate of 130 ml / m².