Coenzyme Q10 preparation for improving myocardial cell activity and cardiovascular function and preparation method thereof

Abstract

The invention relates to the technical field of coenzyme Q10 preparations, and discloses a coenzyme Q10 preparation for improving myocardial cell activity and cardiovascular function, and the coenzyme Q10 preparation comprises the following components in percentage by mass according to 1 liter of prescription: 0.1-10% of coenzyme Q10, 0.5-30% of emulsifier, 5-20% of co-emulsifier, 0.5-10% of stabilizer, 5% of glucose, 0.9% of sodium chloride, 0.25% of absolute ethyl alcohol and the balance of water, according to the coenzyme Q10 preparation for improving the myocardial cell activity and the cardiovascular function, preparation of the coenzyme Q10 preparation is completed, so that the obtained coenzyme Q10 preparation has the functions of enhancing physical ability and vigor, protecting the cardiovascular system, reducing side effects of lipid-lowering drugs, protecting the skin and the like, and the coenzyme Q10 preparation can improve the myocardial function and improve cardiovascular diseases; the heart is prevented from being damaged by oxidative stress.

Description

Technical Field

[0001] The present invention relates to the technical field of coenzyme Q10 preparations, and specifically relates to a coenzyme Q10 preparation for improving myocardial cell viability and cardiovascular function and a preparation method thereof. Background Technique

[0002] Coenzyme Q10 is one of the important physiologically active substances indispensable to human health, and has functions such as improving human immunity, enhancing antioxidant capacity, and delaying aging.

[0003] Since coenzyme Q10 is a yellow or light yellow crystalline powder, odorless, tasteless, easily soluble in chloroform, benzene, carbon tetrachloride, soluble in acetone, petroleum ether and ether, slightly soluble in ethanol, insoluble in water and methanol, it makes the preparation of coenzyme Q10 more difficult. Moreover, after the coenzyme Q10 preparation is taken, it is slowly absorbed in the patient's body and has a very low bioavailability, thus affecting the efficacy of coenzyme Q10 and limiting its wide application. It is urgent to improve this. Summary of the Invention

[0004] The present invention provides a coenzyme Q10 preparation for improving myocardial cell viability and cardiovascular function and a preparation method thereof, which have the advantages of improving myocardial cell viability and cardiovascular function, and solve the problems raised in the above background technique.

[0005] The present invention provides the following technical solution: A coenzyme Q10 preparation for improving myocardial cell viability and cardiovascular function and a preparation method thereof. Based on 1 liter of the prescription, the mass percentage content of the coenzyme Q10 preparation is: coenzyme Q10 0.1%-10%, emulsifier 0.5%-30%, co-emulsifier 5%-20%, stabilizer 0.5%-10%, glucose 5%, sodium chloride 0.9%, absolute ethanol 0.25%, and water makes up the balance.

[0006] As a preferred technical solution of the present invention, it includes a base, an installation frame is installed on the top of the base, a CS120GXL type ultra-high speed centrifuge is installed on the top of the installation frame, a housing is fixedly assembled on the top of the CS120GXL type ultra-high speed centrifuge, a connecting frame is fixedly installed on the top of the installation frame, one end of a bracket is fixedly installed on the outer wall of the installation frame, the other end of the bracket is fixedly assembled with a vertical rotation automatic autoclave, a connecting shaft is fixedly assembled on the power output shaft of the vertical rotation automatic autoclave, and a balance bar is provided on the inner wall of the connecting shaft.

[0007] As a preferred technical solution of the present invention, a preparation chamber is provided on the inner wall of the outer shell. On the outer wall of the preparation chamber, a PHS-2C digital display pH meter, an M-110L microfluidizer, a UV228 ultraviolet-visible light detector, and a Nicomp-380 laser particle size analyzer are respectively provided. At the bottom of the preparation chamber, an AT-130 column thermostat is fixedly assembled. A mixing assembly is provided on the inner wall of the preparation chamber, and a P230 high-pressure constant flow pump is installed on the outer wall of the outer shell.

[0008] As a preferred technical solution of the present invention, an inner cylinder and a high-precision full-automatic AC voltage stabilizer are respectively installed on the inner wall of the preparation chamber. The top of the inner cylinder is rotatably connected to a cylinder cover, and a handle is fixedly assembled on the top of the cylinder cover.

[0009] As a preferred technical solution of the present invention, the mixing assembly includes a servo motor. A rotating shaft is fixedly assembled on the power output shaft of the servo motor. An installation ring is fixedly sleeved on the outer wall of the rotating shaft. A stirrer is fixedly installed on the outer wall of the installation ring. DF-101S heat-collecting constant-temperature heating magnetic bars are fixedly assembled on both outer walls of the stirrer. A high-speed disperser is fixedly assembled on the outer wall of the stirrer away from the installation ring.

[0010] As a preferred technical solution of the present invention, both the stirrer and the high-speed disperser are five in number, and the five stirrers and high-speed dispersers are evenly distributed on the outer wall of the installation ring. The servo motor is electrically connected to the DF-101S heat-collecting constant-temperature heating magnetic bar, the servo motor is electrically connected to the controller, and the top of the servo motor is fixedly installed on the inner wall of the cylinder cover.

[0011] As a preferred technical solution of the present invention, the PHS-2C digital display pH meter, the M-110L microfluidizer, the P230 high-pressure constant flow pump, the UV228 ultraviolet-visible light detector, and the Nicomp-380 laser particle size analyzer are all electrically connected to the controller. The cross-section of the AT-130 column thermostat is trapezoidal, and the top of the AT-130 column thermostat is connected to the bottom of the preparation chamber. The PHS-2C digital display pH meter is electrically connected to the Nicomp-380 laser particle size analyzer.

[0012] As a preferred technical solution of the present invention, the coenzyme Q10 preparation is an orange-yellow clear solution, and the chromatographic conditions of the coenzyme Q10 preparation meet the requirements that the chromatographic column specification is 4.6mm×250mm, 5μm; the mobile phase is n-hexane-ethyl acetate 97:3, the detection wavelength is 275nm; the flow rate is 2.0mL / min; the injection volume is 20μL.

[0013] As a preferred technical solution of the present invention, the stabilizer is one or a mixture of water-soluble starch, sodium carboxymethyl cellulose, hydroxypropyl methylcellulose, carbomer, xanthan gum, arabic gum, and sodium alginate, and the co-emulsifier is one or a mixture of ethanol, propylene glycol, glycerol, or polyethylene glycol.

[0014] A preparation method of a coenzyme Q10 preparation for improving myocardial cell viability and cardiovascular function includes the following steps:

[0015] Step S1: First, weigh LCT or MCT with a mass-volume ratio of 30%, and a mass ratio of 1:1, and heat and melt 2% E80, 0.05% VE, and 2% coenzyme Q10 in a water bath at 60°C until they are uniformly dispersed, and place them on the inner wall of the preparation chamber.

[0016] Step S2: Dissolve 2.25% glycerol and 0.2% GM1 in an appropriate amount of double-distilled water, continue to add it to the inner wall of the preparation chamber, and by controlling the controller, start the servo motor to make the DF-101S heat-collecting constant-temperature heating magnetic stirrer heat the inner wall of the preparation chamber until the temperature of the inner wall of the preparation chamber rises to 60°C, and make the DF-101S heat-collecting constant-temperature heating magnetic stirrer be able to heat the mixed solution on the inner wall of the preparation chamber.

[0017] Step S3: After the temperature rise is completed, the DF-101S heat-collecting constant-temperature heating magnetic stirrer can be rotated driven by the rotating shaft, so that the stirrer can stir the mixed solution on the inner wall of the preparation chamber, and the high-speed disperser can disperse the mixed solution until the solution on the inner wall of the preparation chamber is completely dissolved.

[0018] Step S4: After the two-phase temperature on the inner wall of the preparation chamber is balanced, add the aqueous phase to the oil phase. After complete addition, continue to disperse for 5 minutes to obtain the primary emulsion. After the primary emulsion is cooled to room temperature, add double-distilled water to make up the volume to the prescription amount. Transfer the primary emulsion to an M-110L microfluidizer, homogenize it at 6000 psi for 3 cycles and at 14000 psi for 6 cycles to reduce the particle size, and sequentially pass through 0.80 and 0.45 μm microporous membranes. Charge the obtained emulsion with nitrogen and sterilize it at 121°C for 8 minutes to obtain the coenzyme Q10 preparation.

[0019] The present invention has the following beneficial effects:

[0020] 1. The coenzyme Q10 preparation for improving myocardial cell viability and cardiovascular function is prepared on the inner wall of the preparation chamber, so that the prepared coenzyme Q10 preparation can enhance physical strength and energy, protect the cardiovascular system, reduce the side effects of lipid-lowering drugs, protect the skin, etc. Therefore, it also has important application value in aspects such as improving immunity, delaying aging, health care and beauty, and can enable the coenzyme Q10 preparation to improve myocardial function, treat cardiovascular diseases, and prevent the heart from oxidative stress damage.

[0021] 2. For the coenzyme Q10 preparation for improving myocardial cell viability and cardiovascular function, after the patient takes the prepared coenzyme Q10 preparation, the coenzyme Q10 preparation can provide sufficient oxygen and energy for brain cells, maintain a healthy and active brain and nerve cells, and help delay the onset of Alzheimer's disease and Parkinson's disease. Oxidative stress can affect muscle function, thereby affecting athletic performance. Abnormal mitochondrial function can reduce muscle energy, making it difficult for muscles to contract effectively and maintain movement. Coenzyme Q10 can activate cell respiration, promote cell energy production, and supplementing coenzyme Q10 can significantly enhance the body's vitality, resist fatigue, and thus improve athletic performance. BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 Schematic diagram of the three-dimensional structure of the present invention;

[0023] Figure 2 Schematic diagram of the mounting frame structure of the present invention;

[0024] Figure 3 Schematic diagram of the sectional structure of the present invention;

[0025] Figure 4 Schematic diagram of the mixing component structure of the present invention;

[0026] Figure 5 Schematic diagram of the change in particle size and particle size distribution of the coenzyme Q10 preparation of the present invention after being diluted 50 times with 5% glucose and 0.9% sodium chloride injection;

[0027] Figure 6 Schematic diagram of the photo-degradation curve of the coenzyme Q10 preparation of the present invention;

[0028] Figure 7 Schematic diagram of the photo-degradation curve of the coenzyme Q10 preparation with different dilution multiples of the present invention;

[0029] Figure 8 Schematic diagram of the photo-degradation curve of the coenzyme Q10 preparation with different drug loadings of the present invention.

[0030] In the figure: 1. Base; 2. CS120GXL type ultracentrifuge; 3. Outer shell; 4. Mounting rack; 5. Connecting rack; 6. Bracket; 7. Vertical rotation automatic high-pressure sterilizer; 8. Balance bar; 9. Connecting shaft; 10. Preparation chamber; 11. PHS-2C type digital display pH meter; 12. M-110L type microfluidizer; 13. P230 high-pressure constant-current pump; 14. UV228 ultraviolet-visible light detector; 15. Inner cylinder; 16. Cylinder cover; 17. AT-130 column thermostat; 18. Mixing assembly; 1801. Servo motor; 1802. Rotating shaft; 1803. Mounting ring; 1804. Stirrer; 1805. DF-101S type heat-collecting constant-temperature heating magnetic stir bar; 1806. High-speed disperser; 19. Nicomp-380 laser particle size analyzer; 20. Handle; 21. High-precision fully automatic AC voltage stabilizer. Detailed implementation mode

[0031] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0032] Embodiment 1

[0033] Coenzyme Q10 preparation, the mass percentage content of which is: coenzyme Q10 0.1%-10%, emulsifier 0.5%-30%, co-emulsifier 5%-20%, stabilizer 0.5%-10%, glucose 5%, sodium chloride 0.9%, absolute ethanol 0.25%. Coenzyme Q10 primary emulsions with drug loadings of 0.1% and 2.0% are respectively prepared for photolysis tests, and water is added to make up the balance.

[0034] A preparation method of a coenzyme Q10 preparation for improving myocardial cell viability and cardiovascular function, which includes the following steps:

[0035] Step S1: First, weigh LCT or MCT with a mass-volume ratio of 30% and a mass ratio of 1:1, heat and melt 2% E80, 0.05% VE and 2% coenzyme Q10 uniformly in a 60°C water bath, and place them on the inner wall of the preparation chamber 10.

[0036] Step S2: Dissolve 2.25% glycerol and 0.2% GM1 in an appropriate amount of double-distilled water, continue to add it to the inner wall of the preparation chamber 10, and by controlling the controller, make the servo motor 1801 start to work, so that the DF-101S heat-collecting constant-temperature heating magnetic bar 1805 can heat up the inner wall of the preparation chamber 10 until the temperature of the inner wall of the preparation chamber 10 rises to 60 °C, and make the DF-101S heat-collecting constant-temperature heating magnetic bar 1805 capable of heating the mixed solution on the inner wall of the preparation chamber 10;

[0037] Step S3: After the temperature rise is completed, the DF-101S heat-collecting constant-temperature heating magnetic bar 1805 can be rotated driven by the rotating shaft 1802, so that the stirrer 1804 can stir the mixed solution on the inner wall of the preparation chamber 10, and the high-speed disperser 1806 can disperse the mixed solution until the solution on the inner wall of the preparation chamber 10 is completely dissolved;

[0038] Step S4: After the two-phase temperature on the inner wall of the preparation chamber 10 is balanced, add the aqueous phase to the oil phase. After complete addition, continue to disperse for 5 min to obtain the primary emulsion. After the primary emulsion is cooled to room temperature, add double-distilled water to make up to the prescription volume. Transfer the primary emulsion to the M-110L microfluidizer 12, homogenize it at 6000 psi for 3 cycles and at 14000 psi for 6 cycles to reduce the particle size, and sequentially pass through 0.80 and 0.45 μm microporous membranes. For the obtained emulsion, fill it with nitrogen and sterilize it at 121 °C for 8 min to obtain the coenzyme Q10 preparation.

[0039] As Figure 8 shown, the reaction orders are all first order. The t1 / 2 of the emulsions with 0.1% and 2.0% drug load are 233.5 and 533.1 h respectively. The results show that the photolysis rate is inversely proportional to the drug load. The greater the drug load, the smaller the photolysis rate, and the faster the preparation effect.

[0040] Example Two

[0041] For the coenzyme Q10 preparation, the mass percentage content is: coenzyme Q10 0.1% - 10%, emulsifier 0.5% - 30%, co-emulsifier 5% - 20%, stabilizer 0.5% - 10%, glucose 5%, sodium chloride 0.9%, absolute ethanol 0.25%, and water makes up the balance. Dilute the coenzyme Q10 primary emulsion 0, 2, 4, 10, and 20 times respectively for photolysis tests.

[0042] A preparation method of a coenzyme Q10 preparation for improving myocardial cell viability and cardiovascular function, including the following steps of preparation:

[0043] Step S1: First, weigh LCT or MCT with a mass-volume ratio of 30%, and a mass ratio of 1:1. Heat and melt 2% E80, 0.05% VE, and 2% coenzyme Q10 evenly in a 60°C water bath, and place them on the inner wall of the preparation chamber 10.

[0044] Step S2: Dissolve 2.25% glycerol and 0.2% GM1 in an appropriate amount of double-distilled water, continue to add it to the inner wall of the preparation chamber 10, and by controlling the controller, make the servo motor 1801 start to work, so that the DF-101S heat-collecting constant-temperature heating magnetic stirrer 1805 can heat up the inner wall of the preparation chamber 10 until the temperature of the inner wall of the preparation chamber 10 rises to 60°C, and make the DF-101S heat-collecting constant-temperature heating magnetic stirrer 1805 be able to heat the mixed solution on the inner wall of the preparation chamber 10.

[0045] Step S3: After the temperature rise is completed, the DF-101S heat-collecting constant-temperature heating magnetic stirrer 1805 can be rotated driven by the rotating shaft 1802, so that the stirrer 1804 can stir the mixed solution on the inner wall of the preparation chamber 10, and the high-speed disperser 1806 can disperse the mixed solution until the solution on the inner wall of the preparation chamber 10 is completely dissolved.

[0046] Step S4: After the two-phase temperatures on the inner wall of the preparation chamber 10 are balanced, add the aqueous phase to the oil phase. After complete addition, continue to disperse for 5 minutes to obtain the primary emulsion. After the primary emulsion is cooled to room temperature, add double-distilled water to make up to the prescription volume. Transfer the primary emulsion to the M-110L microfluidizer 12, homogenize it at 6000 psi for 3 cycles and at 14000 psi for 6 cycles to reduce the particle size, and pass through 0.80 and 0.45 μm microporous filters in sequence. For the obtained emulsion, fill it with nitrogen and sterilize it at 121°C for 8 minutes to obtain the coenzyme Q10 preparation.

[0047] As Figure 7 shown, the reaction orders are all first order. The dilution multiples increase from low to high, and the t1 / 2 are 533.1, 495.0, 288.8, 115.5, and 62.4 h respectively, indicating that the higher the dilution degree, the shorter the time to prepare the coenzyme Q10 preparation.

[0048] Example Three

[0049] Coenzyme Q10 preparation, with the following mass percentage contents: coenzyme Q10 0.1% - 10%, emulsifier 0.5% - 30%, co-emulsifier 5% - 20%, stabilizer 0.5% - 10%, glucose 5%, sodium chloride 0.9%, absolute ethanol 0.25%, and water to make up the balance. Dissolve and dilute an appropriate amount of coenzyme Q10 primary emulsion with absolute ethanol to obtain a coenzyme Q10 primary emulsion solution. Weigh 0.25% CoQ10 and 3% HS15, heat and melt them in a water bath at 55°C, disperse evenly, and add preheated injection water at the same temperature under stirring. Stir for 10 minutes to obtain micelles. Prepare coenzyme Q10 primary emulsion solutions, micelles, and emulsions with the same drug concentration for photolysis tests.

[0050] A preparation method of a coenzyme Q10 preparation for improving myocardial cell viability and cardiovascular function, which includes the following steps:

[0051] Step S1: First, weigh LCT or MCT with a mass-volume ratio of 30% and a mass ratio of 1:1, and heat and melt 2% E80, 0.05% VE, and 2% coenzyme Q10 in a water bath at 60°C, disperse evenly, and place them on the inner wall of the preparation chamber 10.

[0052] Step S2: Dissolve 2.25% glycerol and 0.2% GM1 in an appropriate amount of double-distilled water, continue to add it to the inner wall of the preparation chamber 10, and by controlling the controller, make the servo motor 1801 start to work, so that the DF-101S heat-collecting constant-temperature heating magnetic stirrer 1805 can heat the inner wall of the preparation chamber 10 until the temperature of the inner wall of the preparation chamber 10 rises to 60°C, and make the DF-101S heat-collecting constant-temperature heating magnetic stirrer 1805 be able to heat the mixed solution on the inner wall of the preparation chamber 10.

[0053] Step S3: After the temperature rise is completed, make the DF-101S heat-collecting constant-temperature heating magnetic stirrer 1805 rotate driven by the rotating shaft 1802, so that the stirrer 1804 can stir the mixed solution on the inner wall of the preparation chamber 10, and the high-speed disperser 1806 can disperse the mixed solution until the solution on the inner wall of the preparation chamber 10 is completely dissolved.

[0054] Step S4: After the two-phase temperature on the inner wall of the preparation chamber 10 is balanced, add the aqueous phase to the oil phase. After complete addition, continue to disperse for 5 minutes to obtain the primary emulsion. After the primary emulsion is cooled to room temperature, make up the volume to the prescription amount with double-distilled water. Transfer the primary emulsion to the M-110L type microfluidizer 12, homogenize it at 6000 psi for 3 cycles and at 14000 psi for 6 cycles to reduce the particle size, and sequentially pass through 0.80 and 0.45 μm microporous filter membranes. Fill the obtained emulsion with nitrogen and sterilize it at 121°C for 8 minutes to obtain the coenzyme Q10 preparation.

[0055] Such as Figure 6As shown, the reaction order is all first order. The photoprotective effects of coenzyme Q10 preparations from strong to weak are emulsion, micelle and solution, and the t1 / 2 are 239.0, 50.6 and 29.4 h respectively. It can be seen that the stronger the photoprotection, the shorter the preparation time.

[0056] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device comprising a series of elements includes not only those elements, but also other elements not expressly listed, or also elements inherent to such process, method, article or device.

[0057] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A coenzyme Q10 preparation for improving myocardial cell viability and cardiovascular function, characterized in that: Based on 1 liter of the prescription, the coenzyme Q10 preparation has the following mass percentage content: coenzyme Q10 0.1% - 10%, emulsifier 0.5% - 30%, co-emulsifier 5% - 20%, stabilizer 0.5% - 10%, glucose 5%, sodium chloride 0.9%, absolute ethanol 0.25%, and the balance is made up with water.

2. A coenzyme Q10 preparation for improving myocardial cell viability and cardiovascular function according to claim 1, characterized in that: It includes a base (1), and is characterized in that: an installation frame (4) is installed on the top of the base (1), a CS120GXL type ultra-high speed centrifuge (2) is installed on the top of the installation frame (4), a housing (3) is fixedly assembled on the top of the CS120GXL type ultra-high speed centrifuge (2), a connecting frame (5) is fixedly installed on the top of the installation frame (4), one end of a bracket (6) is fixedly installed on the outer wall of the installation frame (4), the other end of the bracket (6) is fixedly assembled with a vertical rotation automatic high-pressure sterilizer (7), a connecting shaft (9) is fixedly assembled on the power output shaft of the vertical rotation automatic high-pressure sterilizer (7), and a balance rod (8) is arranged inside the connecting shaft (9).

3. A coenzyme Q10 preparation for improving myocardial cell viability and cardiovascular function according to claim 2, characterized in that: A preparation chamber (10) is arranged on the inner wall of the housing (3). A PHS-2C type digital display pH meter (11), an M-110L type microfluidizer (12), a UV228 ultraviolet-visible light detector (14), and a Nicomp-380 laser particle size analyzer (19) are respectively arranged on the outer wall of the preparation chamber (10). An AT-130 column temperature box (17) is fixedly assembled at the bottom of the preparation chamber (10). A mixing assembly (18) is arranged on the inner wall of the preparation chamber (10). A P230 high-pressure constant current pump (13) is installed on the outer wall of the housing (3).

4. A coenzyme Q10 preparation for improving myocardial cell viability and cardiovascular function according to claim 3, characterized in that: An inner cylinder (15) and a high-precision full-automatic AC voltage stabilizer (21) are respectively installed on the inner wall of the preparation chamber (10). A cylinder cover (16) is rotatably connected to the top of the inner cylinder (15). A handle (20) is fixedly assembled on the top of the cylinder cover (16).

5. A coenzyme Q10 preparation for improving myocardial cell viability and cardiovascular function according to claim 3, characterized in that: The mixing assembly (18) includes a servo motor (1801). A rotating shaft (1802) is fixedly assembled on the power output shaft of the servo motor (1801). An installation ring (1803) is fixedly sleeved on the outer wall of the rotating shaft (1802). A stirrer (1804) is fixedly installed on the outer wall of the installation ring (1803). DF-101S heat-collecting type constant temperature heating magnetic bars (1805) are fixedly assembled on both outer walls of the stirrer (1804). A high-speed disperser (1806) is fixedly assembled on the outer wall of the stirrer (1804) away from the installation ring (1803).

6. The coenzyme Q10 preparation for improving myocardial cell viability and cardiovascular function according to claim 5, wherein: The number of the stirrers (1804) and the high-speed dispersers (1806) is five each, and the five stirrers (1804) and the high-speed dispersers (1806) are evenly distributed on the outer wall of the installation ring (1803). The servo motor (1801) is electrically connected to the DF-101S heat-collecting type constant temperature heating magnetic bars (1805). The servo motor (1801) is electrically connected to a controller. The top of the servo motor (1801) is fixedly installed on the inner wall of the cylinder cover (16).

7. A coenzyme Q10 preparation for improving myocardial cell viability and cardiovascular function according to claim 3, characterized in that: The PHS-2C digital display pH meter (11), M-110L microfluidizer (12), P230 high-pressure constant current pump (13), UV228 ultraviolet-visible detector (14), and Nicomp-380 laser particle size analyzer (19) are all electrically connected to the controller. The cross-section of the AT-130 column oven (17) is trapezoidal, and the top of the AT-130 column oven (17) is connected to the bottom of the preparation chamber (10). The PHS-2C digital display pH meter (11) is electrically connected to the Nicomp-380 laser particle size analyzer (19).

8. A coenzyme Q10 preparation for improving myocardial cell viability and cardiovascular function according to claim 1, characterized in that: The coenzyme Q10 preparation is an orange-yellow clear solution, and the chromatographic conditions of the coenzyme Q10 preparation meet the specifications of the chromatographic column: 4.6 mm × 250 mm, 5 μm; the mobile phase is n-hexane-ethyl acetate 97:3, the detection wavelength is 275 nm; the flow rate is 2.0 mL / min; the injection volume is 20 μL.

9. A coenzyme Q10 preparation for improving myocardial cell viability and cardiovascular function according to claim 1, characterized in that: The stabilizer is one or a mixture of water-soluble starch, sodium carboxymethyl cellulose, hydroxypropyl methylcellulose, carbomer, xanthan gum, arabic gum, and sodium alginate. The co-emulsifier is one or a mixture of ethanol, propylene glycol, glycerol, or polyethylene glycol.

10. The preparation method of a coenzyme Q10 preparation for improving myocardial cell viability and cardiovascular function according to claim 1, characterized in that, It is prepared by including the following steps: Step S1: First, weigh LCT or MCT with a mass-volume ratio of 30%, and a mass ratio of 1:1, so that 2% E80, 0.05% VE, and 2% coenzyme Q10 are heated and melted in a 60°C water bath, dispersed evenly, and placed on the inner wall of the preparation chamber (10). Step S2: Dissolve 2.25% glycerol and 0.2% GM1 in an appropriate amount of double-distilled water, continue to add it to the inner wall of the preparation chamber (10), and by controlling the controller, start the servo motor (1801) to work, so that the DF-101S heat-collecting constant-temperature heating magnetic bar (1805) can heat the inner wall of the preparation chamber (10) until the temperature of the inner wall of the preparation chamber (10) rises to 60°C, and the DF-101S heat-collecting constant-temperature heating magnetic bar (1805) can heat the mixed solution on the inner wall of the preparation chamber (10). Step S3: After the temperature rise is completed, the DF-101S heat-collecting constant-temperature heating magnetic bar (1805) can be rotated driven by the rotating shaft (1802), so that the stirrer (1804) can stir the mixed solution on the inner wall of the preparation chamber (10), and the high-speed disperser (1806) can disperse the mixed solution until the solution on the inner wall of the preparation chamber (10) is completely dissolved. Step S4: After the two-phase temperature on the inner wall of the preparation chamber (10) is balanced, add the aqueous phase to the oil phase. After complete addition, continue to disperse for 5 min to obtain the primary emulsion. After the primary emulsion is cooled to room temperature, add double-distilled water to make up to the prescription volume. Transfer the primary emulsion to the M-110L microfluidizer (12), homogenize it at 6000 psi for 3 cycles and 14000 psi for 6 cycles to reduce the particle size, and sequentially pass through 0.80 and 0.45 μm microporous membranes. Fill the obtained emulsion with nitrogen and sterilize it at 121°C for 8 min to obtain the coenzyme Q10 preparation.

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