Intelligent mouse cage for mouse chronic unpredictable stimulation model and experimental method

Abstract

The invention belongs to the technical field of animal experiment instruments, and relates to an intelligent mouse cage for a mouse chronic unpredictable stimulation model and an experiment method. The mouse cage comprises an upper-layer cage body and a base, the upper-layer cage body is composed of an upper cover, a lower wall and a side wall, a stimulation module is arranged in the mouse cage, the stimulation module comprises a temperature stimulation module, a humidity stimulation module, an acousto-optic stimulation module, an inclined vibration stimulation module, a water and grain control module and a space stimulation module, and the stimulation module is controlled by a central controller; the intelligent mouse cage is compact in structure, and stimulation conditions can be automatically controlled. Moreover, various unpredictable stimulation can be completed in a single device, the intelligent mouse cage is easy to maintain and clean, and whether modeling succeeds or not is conveniently checked, so that the mouse welfare is ensured, the accuracy and repeatability of experimental data are improved, and manpower and experimental expenditure are effectively saved.

Description

Technical Field

[0001] The present invention belongs to the technical field of animal experiment instruments, and relates to an intelligent mouse cage and an experimental method for a mouse chronic unpredictable stimulation model. Background Art

[0002] Depression is a common mental illness globally, with a high recurrence rate, seriously affecting the quality of human life and being the main cause of mental disability worldwide. To deeply study the pathogenesis of depression and develop effective treatment methods, scientists have developed various animal models, among which the CUMS model is widely used because it can better simulate the pathogenesis process of human depression. By applying a series of unpredictable mild stressors to experimental animals, this model can induce depression-like behavioral and physiological changes, thus helping researchers better understand the pathogenesis of depression and evaluate the efficacy of drug treatment. The stressors of the CUMS model usually include various different types of stimuli, such as food or water deprivation, increased cage humidity, noise exposure, temperature change, photoperiod alteration, cage tilt or vibration, activity restriction. These stressors are randomly applied to experimental animals, keeping them in a continuous stress state. This continuous and unpredictable stress is the core feature of the CUMS model and also makes it an effective tool for studying depression.

[0003] Existing CUMS experiments have high requirements for manpower, space, and cost, and have deficiencies in stimulus connection, animal escape prevention and control, and experimental accuracy; at the same time, the scattered use of different stimulus devices also leads to greater complexity in maintenance, cleaning, and operation, increasing the error and time cost of the experimental process. Therefore, there is an urgent need to design an intelligent mouse cage for a mouse chronic unpredictable stress model. Summary of the Invention

[0004] The purpose of the present invention is to overcome the above-mentioned disadvantages of the existing technology and propose an intelligent mouse cage and an experimental method for a mouse chronic unpredictable stimulation model.

[0005] To achieve the above purpose, the present invention adopts the following technical solutions:

[0006] An intelligent mouse cage for a mouse chronic unpredictable stimulation model, comprising a mouse cage, the mouse cage includes an upper cage body and a base, the upper cage body is composed of an upper cover, a lower wall, and side walls, a stimulation module is arranged in the mouse cage, the stimulation module includes a temperature stimulation module, a humidity stimulation module, an acoustic and optical stimulation module, a tilt and vibration stimulation module, a water and food control module, a space stimulation module, and the stimulation module is controlled by a central controller; a control panel is arranged on the top wall of the upper cage body;

[0007] The temperature stimulation module includes a heating sheet and a cooling sheet arranged on the top wall of the base. The heating inside the mouse cage is realized through the heating sheet, and the cooling inside the mouse cage is realized through the cooling sheet. The humidity stimulation module includes a humidifier arranged outside the mouse cage, and humidification is carried out inside the mouse cage through the humidifier. The sound and light stimulation module includes a sound source installed on the side wall of the mouse cage, and noise is released through the sound source. The sound and light stimulation module also includes a controllable light source arranged on the upper cover, and the change of light is realized through the controllable light source. The tilt and vibration stimulation module includes a vibration tilt platform arranged on the base, and the vibration tilt platform realizes the tilt or vibration of the vibration tilt platform by changing the angle and speed of the output shaft of the shaker motor. The water and food control module includes a food trough arranged under the upper cover and a water bottle arranged outside the side wall of the mouse cage. The space stimulation module includes a first partition board and a second partition board oppositely arranged inside the mouse cage, and the size of the space is changed by changing the relative distance between the first partition board and the second partition board.

[0008] Further, the base includes a bottom plate and a hinge head arranged on the base. A connecting block is arranged on the top wall of the base, and the connecting block is hinged to the hinge head. The tilt and vibration stimulation module includes a shaker motor located on the bottom plate. The shaker motor is connected to the bottom plate through a shaker, and first electric rods are arranged at both the left and right output ends of the shaker motor.

[0009] Further, the vibration frequency, tilt angle, and switching duration of the shaker motor can all be adjusted according to the set program or random algorithm in the central control system.

[0010] Further, the water and food control module includes a second electric rod arranged on the side wall. The output end of the second electric rod is connected to a connecting rod, and the connecting rod is connected to a food trough rotating rod. A food trough is sleeved on the food trough rotating rod.

[0011] Further, the water and food control module includes a water bottle bracket arranged on the outer side wall of the cage body. The water bottle bracket includes a bracket rotating rod, a water bottle baffle is arranged on the bracket rotating rod, and a water bottle is arranged on the water bottle baffle. The water and food control module also includes a third electric rod. One end of the third electric rod is connected to the bracket rotating rod, and the other end of the third electric rod is connected to the side wall of the cage body.

[0012] Further, the space stimulation module includes a first motor arranged on the side wall. The output end of the first motor is connected to a support rod, and threads are arranged on the support rod. The first partition board and the second partition board are sleeved on the support rod, and the first motor drives the support rod to rotate, thereby driving the first partition board and the second partition board to move.

[0013] Further, the base is provided with an inner cavity, and the temperature stimulation module includes a temperature control area located in the inner cavity. The temperature control area is located below the cage body. A heating sheet and a refrigerating sheet are arranged in the temperature control area. A temperature sensor is arranged in the cage body. A temperature signal is sent to the central controller through the temperature sensor, and the temperature control area is controlled by the central controller to achieve refrigeration or heating.

[0014] Further, the temperature stimulation module includes a gas ventilation fan located on the upper cover, and the gas ventilation fan is controlled by the central controller.

[0015] Further, the humidity stimulation module includes a humidifier installed outside the side wall, and the start of the humidifier is controlled by the central controller.

[0016] Further, the sound and light stimulation module includes a sound source installed on the inner side of the side wall and a controllable light source installed on the upper cover, and the sound source and the controllable light source are controlled by the central controller.

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] The present invention provides an intelligent mouse cage for a chronic unpredictable stimulation model of mice. The structure of the intelligent mouse cage is compact, and the stimulation conditions can be automatically controlled. Moreover, it is an intelligent mouse cage that can complete a variety of unpredictable stimulations in a single device, is easy to maintain and clean, and is convenient for checking whether the modeling is successful, so as to improve the accuracy and repeatability of experimental data while ensuring the welfare of mice, and effectively save manpower and experimental funds. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] The drawings here are incorporated into the specification and form a part of this specification, and are used together with the specification to explain the principle of the present invention.

[0020] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings required for use in the description of the embodiments or the prior art. Obviously, for those of ordinary skill in the art, other drawings can also be obtained according to these drawings without creative efforts.

[0021] Figure 1 It is a schematic diagram of the overall structure of the present invention;

[0022] Figure 2 It is an axial view of the overall structure of the present invention;

[0023] Figure 3 It is a transverse sectional view of the present invention;

[0024] Figure 4 It is a view of part I of the present invention;

[0025] Figure 5 View of part II of the present invention;

[0026] Figure 6 Schematic diagram of the heating sheet of the present invention;

[0027] Figure 7 Side view of the present invention;

[0028] Figure 8 Schematic diagram of the water bottle baffle of the present invention.

[0029] Wherein: 1 is the side wall; 2 is the lower wall; 201 is the upper layer electric column slot; 3 is the bottom plate; 301 is the rocker column; 4 is the top wall of the base; 401 is the lower layer electric column; 5 is the rocker motor; 6 is the first electric rod; 7 is the rocker seat; 8 is the upper and lower layer connection buckle; 9 is the buckle slot; 10 is the observation window cover; 11 is the heating sheet; 12 is the cooling sheet; 13 is the movable rod; 14-1 is the first enclosure; 14-2 is the second enclosure; 15 is the rack rod; 16 is the first motor; 17 is the feeding trough; 18 is the second electric rod; 19 is the feeding trough cover; 20 is the sound source; 21 is the humidifier; 22 is the ventilation plate; 23 is the control panel; 24 is the heater; 25 is the gas ventilation fan; 26 is the air filter element; 27 is the ventilation fan cover; 28 is the third electric rod; 29 is the water bottle; 30 is the water bottle bracket; 31 is the water bottle baffle; 32 is the feeding trough rotating rod; 33 is the controllable light source; 34 is the bracket rotating rod. Detailed implementation mode

[0030] Here, the exemplary embodiments will be described in detail. The implementation manners described in the following exemplary embodiments do not represent all implementation manners consistent with the present invention. On the contrary, they are merely examples consistent with some aspects of the present invention detailed in the appended claims.

[0031] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention will be further described in detail below with reference to the drawings and embodiments.

[0032] Embodiment

[0033] As Figures 1 - 2 shown, an intelligent mouse cage for a mouse chronic unpredictable stimulation model includes a mouse cage. The mouse cage includes an upper cage body and a base. The upper cage body is composed of an upper cover, a lower wall 2 and a side wall 1. A stimulation module is arranged in the mouse cage. The stimulation module includes a temperature stimulation module, a humidity stimulation module, an acoustic and optical stimulation module, an inclination and vibration stimulation module, a water and food control module, and a space stimulation module. The stimulation module is controlled by a central controller; a control panel 23 is arranged on the top wall of the upper cage body;

[0034] The temperature stimulation module includes a heating sheet 11 and a cooling sheet 12. The heating inside the mouse cage is achieved through the heating sheet 11, and the cooling inside the mouse cage is achieved through the cooling sheet 12. The humidity stimulation module includes a humidifier 21 arranged outside the mouse cage, and humidification is carried out inside the mouse cage through the humidifier 21. The sound and light stimulation module includes a sound source 20 installed on the side wall of the mouse cage, and noise is released through the sound source 20. The sound and light stimulation module also includes a controllable light source 33 arranged on the upper cover, and the change of light is achieved through the controllable light source 33. The tilt vibration stimulation module includes a vibration tilt platform arranged on the base, and the tilt or vibration of the vibration tilt platform is achieved by changing the angle and speed of the output shaft of the shaker motor 5. The water and food control module includes a food trough 17 arranged below the upper cover and a water bottle 29 arranged outside the side wall of the mouse cage. The space stimulation module includes a first partition 14-1 and a second partition 14-2 oppositely arranged inside the mouse cage, and the size of the space is changed by changing the relative distance between the first partition 14-1 and the second partition 14-2.

[0035] In this embodiment, the intelligent mouse cage includes at least the following parts:

[0036] Cage structure: The overall structure is a cube or a cuboid, composed of an upper-layer cage body and a base, and the internal space for the activities of mice is accommodated. As Figure 5 shown, a ventilation plate 22 is provided at the top of the upper-layer cage body. The ventilation plate 22 is composed of a control panel 23 and a gas ventilation device. The gas ventilation device is composed of a gas ventilation fan 25, an air filter element 26 and a ventilation fan cover 27. A control panel 23 is also provided on the ventilation plate 22, and a heater 22 is provided at the bottom of the ventilation plate 22. The base and the cage body are connected through an upper and lower layer connection buckle 8 and a buckle groove 9. The side wall 1 of the cage body is made of an opaque material, which is convenient for controlling the light cycle. An observation window cover 10 is provided on the side wall 1, and the activity state of the mice can be observed. The upper cover is provided with a gas ventilation fan and an air filter element, which are used to keep the air flowing and meet the hot air stimulation requirements.

[0037] Multi-functional programmable stimulation components:

[0038] (1) Temperature stimulation module: Integrate controllable cold and hot modules in the base, and realize real-time monitoring and adjustment of the temperature inside the cage through a temperature sensor and a central control system, and can automatically switch between cold / hot stimulation according to a random or preset program;

[0039] (2) Humidity / immersion stimulation module: Reserve a water tank outside the side wall for humidifying the area inside the cage to form a humid environment during a specified period.

[0040] (3) Acoustic and light stimulation module: Programmable sound sources and controllable light sources are respectively installed on the side wall and the upper cover of the cage body, and different frequencies and intensities of acoustic and light stimulations are generated through external random programs or preset programs to change the light rhythm.

[0041] (4) Vibration / Tilt platform module: A micro platform that can vibrate or tilt is set at the base of the mouse cage and is driven by a motor, which can change the tilt angle or vibration frequency to create mechanical stimulation.

[0042] (5) Spatial stimulation module: The activity space of the mice is temporarily partitioned by setting a movable first partition 14-1 and a second partition 14-2 inside the cage to achieve activity restriction or change the living space environment.

[0043] (6) Water and food control module: By setting a rotatable food trough 17 and a water bottle 29, water and food prohibition without human operation is achieved. At the same time, the setting of two water bottles 29 is convenient for testing during the sugar water experiment, and the mechanical feeding in and out can also greatly reduce the error of manual operation.

[0044] (7) Sensor and monitoring system: It includes a variety of sensors such as temperature, humidity, light intensity, noise, and real-time video monitoring; data can be read through the sensors and transmitted to the operation panel of the top cover control system to adjust the stimulation mode in real time and record the experimental data.

[0045] (8) Central control system: It is configured with a programmable controller (such as an embedded processor or a single-chip microcomputer) and a random algorithm module to achieve the following functions:

[0046] a. Automatic arrangement of unpredictable stimulations: Automatically switch the working states and intensities of various stimulation units according to the set time period, random algorithm, or custom program.

[0047] b. Data recording and remote monitoring: Real-time record the temperature, humidity, acoustic and light environment, vibration frequency, and mouse behavior, and can be linked with a computer or a mobile terminal through wired or wireless means to achieve remote monitoring and parameter adjustment.

[0048] c. Safety protection mechanism: Automatically stop or switch the stimulation mode when sensor abnormalities or system failures occur to protect the experimental animals and avoid data distortion.

[0049] (9) Base integrated storage and electric column connection:

[0050] Modular design: The power supply, tilt and vibration stimulation module, etc. are uniformly stored inside the base, and a detachable structure is set for convenient maintenance and repair; it is connected to the upper layer through an electric column for quick use.

[0051] Through the above structural and functional integration, the intelligent mouse cage of the present invention realizes the automated integrated control of multiple stressors, overcoming the defects of existing CUMS experimental equipment in terms of cumbersome operation, animal escape, discontinuous stimulation connection, and high maintenance costs.

[0052] In summary, the purpose of the present invention is to provide an intelligent mouse cage with a compact structure, automatic control of stimulation conditions, capable of completing multiple unpredictable stimulations within a single device, easy to maintain and clean, and convenient for verifying the success of modeling, so as to improve the accuracy and repeatability of experimental data while ensuring the welfare of mice, and effectively save manpower and experimental funds.

[0053] Further, as Figures 3 - 4 shown, the base includes a bottom plate 3 and a hinge head provided on the base 3. A connecting block is provided on the top wall 4 of the base, and the connecting block is hinged to the hinge head. The tilt vibration stimulation module includes a shaker motor 5 located on the bottom plate 3. The shaker motor 5 is connected to the bottom plate through a shaker seat 7. First electric rods 6 are provided at both the left and right output ends of the shaker motor 5. A lower electric column 401 is provided on the top wall 4 of the base, and the lower electric column 401 is matched with an upper electric column slot 401 on the lower wall 2. The function of the lower electric column 401 is to supply power to the central control system and all modules.

[0054] Further, the vibration frequency, tilt angle, and switching duration of the shaker motor 5 can be adjusted according to the set program or random algorithm in the central control system.

[0055] In this embodiment: The vibration / tilt platform provided in the base is driven by the shaker motor 5, and the tilt or vibration is achieved by changing the angle and speed of the output shaft.

[0056] When the system enters the vibration stimulation mode, the shaker motor 5 in combination with the first electric rod 6 will vibrate at a predetermined or random frequency and amplitude; if it enters the tilt mode, the platform will slowly or quickly change the tilt angle, making the mouse in an unstable environment.

[0057] The vibration frequency, tilt angle, and switching duration can be adjusted in real time according to the set program or random algorithm in the central control system to ensure the satisfaction of the unpredictability requirements of the CUMS experiment.

[0058] When the tilt is too large or the vibration is too strong, the central controller will automatically limit the amplitude or issue a warning to prevent excessive stress or harm to the experimental animals.

[0059] Further, the water and food control module includes a second electric rod 18 provided on the side wall 1. The output end of the second electric rod 18 is connected to a connecting rod, and the connecting rod is connected to a food trough rotating rod 32. A food trough 17 is sleeved on the food trough rotating rod 32, and a food trough cover 19 is provided on the food trough 17.

[0060] Further, as Figures 7 - 8 shown, the water and food control module includes a water bottle holder 30 disposed on the outer side wall of the cage body. The water bottle holder 30 includes a support rotating rod 34. A water bottle baffle 31 is disposed on the support rotating rod 34. A water bottle 29 is disposed on the water bottle baffle 31. The water and food control module further includes a third electric rod 28. One end of the third electric rod 28 is connected to the support rotating rod, and the other end of the third electric rod 28 is connected to the side wall of the cage body.

[0061] In this embodiment: The food trough 17 and the water bottle 29 in the mouse cage can open or close the water intake / food intake passage through a motor or a mechanical switch, realizing unmanned water and food restriction operations.

[0062] The central control module closes the food trough 17 regularly or randomly according to experimental requirements and random programs to simulate the stress state of water shortage and food shortage.

[0063] Sucrose water experiment test

[0064] When it is necessary to conduct a sucrose water experiment to evaluate whether the modeling is successful, two water bottles 29 can be set in the cage, one for pure water and the other for sucrose water. The drinking volume and preference of the mice are recorded through sensors.

[0065] Mechanically sending in or removing the water bottle can reduce the interference of personnel operations and improve the accurate monitoring of the intake behavior of mice.

[0066] Further, the space stimulation module includes a first motor 16 disposed on the side wall. The output end of the first motor is connected with a support rod 15. Threads are disposed on the support rod 15. The first enclosure and the second enclosure are sleeved on the support rod 15. By driving the support rod 15 to rotate through the first motor 16, the first enclosure 14-1 and the second enclosure 14-2 are driven to move.

[0067] In this embodiment: Movable first enclosure 14-1, second enclosure 14-2, support rod 15, etc. are provided inside the mouse cage to realize the restriction of the activity space of the mice. The two enclosures can be manually or automatically moved to the designated position by a small motor. To keep the first enclosure 14-1 and the second enclosure 14-2 stable, movable rods 13 arranged in a cross shape are respectively installed on one side of the first enclosure 14-1 and the second enclosure 14-2. The movable rods 13 can be telescoped through the cross structure. One end of the movable rod 13 is fixed to the lower wall and the side wall of the upper cage body.

[0068] The activation of the two enclosures can be used in combination with other stimuli (such as humidity or noise) to generate different stimulation environments in the same space and realize multiple stresses on the mice.

[0069] Further, the base is provided with an inner cavity, and the temperature stimulation module includes a temperature control area located in the inner cavity, and the temperature control area is located below the cage body. As Figure 6 shown, a heating sheet 11 and a cooling sheet 12 are arranged in the temperature control area, a temperature sensor is arranged in the cage body, a temperature signal is sent to the central controller through the temperature sensor, and the temperature control area is controlled by the central controller to realize refrigeration or heating.

[0070] Further, the temperature stimulation module includes a gas ventilation fan 25 located on the upper cover, and the gas ventilation fan 25 is controlled by the central controller.

[0071] Further, the humidity stimulation module includes a humidifier 21 installed on the outer side of the side wall, and the start of the humidifier 21 is controlled by the central controller.

[0072] In this embodiment: Temperature and humidity sensors are distributed in the cage body, and the detection signals are fed back to the central control system in real time. The control system dynamically adjusts the stimulation scheme according to the sensor data. For example, if the humidity is higher than the safety threshold, humidification is immediately stopped and ventilation and drying are started; if the temperature continues to rise, heating is reduced and refrigeration is started.

[0073] Further, the sound and light stimulation module includes a sound source 20 installed on the inner side of the side wall and a controllable light source 33 installed on the upper cover, and the sound source 20 and the controllable light source 33 are controlled by the central controller.

[0074] In this embodiment: The upper cover is provided with a controllable light source, which can realize stimulations such as flashing, sudden bright light, and changing light cycle, and can also simulate the change of circadian rhythm. The central controller can set or randomly change the brightness and switching frequency of the light source, and record the light intensity for subsequent analysis. For the intensity of noise and light stimulation, it can be monitored by the built-in sensor and fed back to the central controller to timely adjust the output parameters or trigger other associated stimulations.

[0075] The sound source installed on the side wall 1 can output noises with different intensities, frequencies or intermittent modes (such as white noise, noise, etc.) within a specified period of time to create an unpredictable sound stimulation environment.

[0076] The central controller automatically controls the noise duration, loudness and cycle frequency according to a random algorithm to ensure that the mice cannot predict the occurrence of sound stimulation.

[0077] The above are only specific embodiments of the present invention, which enable those skilled in the art to understand or implement the present invention. Various modifications to these embodiments will be obvious to those skilled in the art, and the general principles defined herein can be implemented in other embodiments without departing from the spirit or scope of the present invention.

[0078] It should be understood that the present invention is not limited to the above-described content and various modifications and changes can be made without departing from its scope. The scope of the present invention is only limited by the appended claims.

Claims

1. An intelligent mouse cage for a chronic unpredictable stimulation model of mice, characterized in that, It includes a squirrel cage, and the squirrel cage includes an upper cage body and a base. The upper cage body is composed of an upper cover, a lower wall (2) and a side wall (1). A stimulation module is arranged inside the squirrel cage. The stimulation module includes a temperature stimulation module, a humidity stimulation module, an acoustic and optical stimulation module, an inclined vibration stimulation module, a water and food control module, and a space stimulation module. The stimulation module is controlled by a central controller; a control panel (23) is arranged on the top wall of the upper cage body; The temperature stimulation module includes a heating sheet (11) and a refrigerating sheet (12) arranged on the top wall (4) of the base. The internal heating of the squirrel cage is realized through the heating sheet (11), and the internal refrigeration of the squirrel cage is realized through the refrigerating sheet (12); the humidity stimulation module includes a humidifier (21) arranged outside the squirrel cage, and humidification is carried out inside the squirrel cage through the humidifier (21); the acoustic and optical stimulation module includes a sound source (20) installed on the side wall of the squirrel cage, and noise is released through the sound source (20). The acoustic and optical stimulation module also includes a controllable light source (33) arranged on the upper cover, and the change of light is realized through the controllable light source (33); the inclined vibration stimulation module includes a vibration inclined platform arranged on the base, and the vibration inclined platform realizes the inclination or vibration of the vibration inclined platform by changing the angle and speed of the output shaft of the rocker motor (5); the water and food control module includes a feeding trough (17) arranged below the upper cover and a water bottle (29) arranged outside the side wall of the squirrel cage; the space stimulation module includes a first partition board (14-1) and a second partition board (14-2) oppositely arranged inside the squirrel cage, and the first partition board (14-1) and the second partition board (14-2) realize the change of the size of the space by changing the relative distance.

2. The intelligent mouse cage for the chronic unpredictable stimulation model of mice according to claim 1, wherein The base includes a bottom plate (3) and a hinge joint (301) arranged on the base (3). A connecting block (402) is arranged on the top wall (4) of the base, and the connecting block (402) is hinged to the hinge joint (301). The inclined vibration stimulation module includes a rocker motor (5) located on the bottom plate (3), and the rocker motor (5) is connected to the bottom plate through a rocker seat (7). First electric rods (6) are arranged at both the left and right output ends of the rocker motor (5).

3. The intelligent mouse cage for a mouse chronic unpredictable stimulation model according to claim 1, characterized in that The vibration frequency, inclination angle and switching duration of the rocker motor (5) can be adjusted according to the set program or random algorithm in the central control system.

4. The intelligent mouse cage for a chronic unpredictable stimulation model of mice according to claim 1, characterized in that, The water and food control module includes a second electric rod (18) arranged on the side wall (1). The output end of the second electric rod (18) is connected with a connecting rod, and the connecting rod is connected to a feeding trough rotating rod (32). A feeding trough (17) is sleeved on the feeding trough rotating rod (32).

5. The intelligent mouse cage for a chronic unpredictable stimulation model of mice according to claim 1, characterized in that, The water and food control module includes a water bottle holder (30) provided on the outer side wall of the cage body. The water bottle holder (30) includes a holder rotating rod (34). A water bottle baffle (31) is provided on the holder rotating rod (34). A water bottle (29) is provided on the water bottle baffle (31). The water and food control module further includes a third electric rod (28). One end of the third electric rod (28) is connected to the holder rotating rod, and the other end of the third electric rod (28) is connected to the side wall of the cage body.

6. The intelligent mouse cage for a chronic unpredictable stimulation model of mice according to claim 1, characterized in that, The space stimulation module includes a first motor (16) provided on the side wall. The output end of the first motor is connected to a support rod (15). Threads are provided on the support rod (15). The first enclosure and the second enclosure are sleeved on the support rod (15). By driving the support rod (15) to rotate through the first motor (16), the first enclosure (14-1) and the second enclosure (14-2) are driven to move.

7. The intelligent mouse cage for a chronic unpredictable stimulation model of mice according to claim 1, characterized in that, The base is provided with an inner cavity. The temperature stimulation module includes a temperature control area located in the inner cavity. The temperature control area is located below the cage body. A heating sheet (11) and a refrigerating sheet (12) are provided in the temperature control area. A temperature sensor is provided in the cage body. A temperature signal is sent to the central controller through the temperature sensor. The temperature control area is controlled by the central controller to achieve refrigeration or heating.

8. The intelligent mouse cage for a chronic unpredictable stimulation model of mice according to claim 7, characterized in that, The temperature stimulation module includes a gas ventilation fan (25) located on the upper cover. The gas ventilation fan (25) is controlled by the central controller. The humidity stimulation module includes a humidifier (21) installed outside the side wall. The start of the humidifier (21) is controlled by the central controller.

9. The intelligent mouse cage for a mouse chronic unpredictable stimulation model according to claim 1, wherein The sound and light stimulation module includes a sound source (20) installed on the inner side of the side wall and a controllable light source (33) installed on the upper cover. The sound source (20) and the controllable light source (33) are controlled by the central controller.

10. An experimental method applied to a mouse chronic unpredictable stress model, characterized in that, It includes an intelligent mouse cage for a mouse chronic unpredictable stimulation model according to any one of claims 1 to 9. Place the intelligent mouse cage for a mouse chronic unpredictable stimulation model in a quiet room, and place a mouse in the upper cage body. Select a suitable stimulation method through the control panel (23) according to the experimental requirements to conduct a stimulation experiment on the mouse.

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