Automatic sterile excrement sampling and freezing storage system suitable for clinical examination

Abstract

An automatic sterile excrement sampling and freezing storage system suitable for clinical examination relates to the technical field of excrement sampling, and comprises a workbench, and a sampling device, a sample retention device, a refrigeration device and a data acquisition and control device which are mounted on the workbench, the sample indwelling device is used for placing a sample to be taken; the sampling device is used for obtaining a to-be-taken sample in the sample retention device; a plurality of sample indwelling devices and a plurality of sampling devices are arranged, and the plurality of sample indwelling devices and the plurality of sampling devices are in one-to-one correspondence; the refrigerating device is used for cooling a sample obtained in the sampling device; the data acquisition and control device is used for acquiring data of a sample to be taken in the sample retention device and sample data acquired in the sampling device, and automatically controlling the sample retention device, the sampling device and the refrigerating device to cooperatively execute sampling operation according to the acquired data; the method is used for solving the problems of low sampling efficiency, high pollution risk and unstable sample quality.

Description

Technical Field

[0001] The present invention relates to the technical field of fecal sampling, and particularly relates to an automated sterile fecal sampling and cryogenic storage system suitable for clinical tests. Background Art

[0002] In the field of clinical medical research, the collection, preservation, and analysis of fecal samples are of great significance for gut microbiome research, disease diagnosis (such as intestinal infections, inflammatory bowel disease, colorectal cancer screening, etc.), and drug metabolism research.

[0003] Traditional fecal sampling methods usually rely on manual operations, but there are often many problems, including: the sampling operation is cumbersome, which may cause contamination and affect the accuracy of test results; the sample preservation is unstable, and the microbial community and metabolites in fecal samples are prone to change at room temperature, while the existing refrigeration methods usually rely on low-temperature storage in laboratories and cannot be instantaneously frozen after collection, affecting the reliability of experimental data; the risk of cross-contamination, because different patients' samples need to be stored separately, traditional sampling methods may cause contamination due to container replacement or operation errors, affecting test results; the degree of automation is low, and most existing fecal sampling and storage devices are manually operated and cannot meet the requirements of batch and standardized clinical tests.

[0004] Chinese Patent with Publication No. CN106442007A discloses a fecal sampling device. When sampling, hold the push rod of the piston and insert the sampling port of the sampling tube into the sample. The sample enters the sampling tube from the sampling port. After the amount of the sample entering meets the requirement, take out the sampling tube containing the sample and put it into the protective shell for sealing and transportation. And because the sampling tube is sealed in the protective shell after sampling, the sample is isolated from the air outside the protective shell, alleviating the technical problem of contamination caused by the sample contacting the air during long-distance transportation; however, this device cannot solve the above problems of unstable sample preservation and low degree of automation; Therefore, there is an urgent need for a system that can achieve automated fecal sampling and timely cryogenic storage. Summary of the Invention

[0005] The purpose of the present invention is to provide an automated sterile fecal sampling and cryogenic storage system suitable for clinical tests, which is used to solve the problems of low sampling efficiency, high pollution risk, and unstable sample quality.

[0006] The present invention is achieved through the following technical solutions: An automated sterile fecal sampling and cryogenic storage system suitable for clinical tests includes a workbench, and a sampling device, a sample retention device, a refrigeration device, and a data acquisition and control device installed on the workbench; The sample retention device is used to place the sample to be taken; The sampling device is used to obtain the sample to be taken in the sample retention device; Both the sample retention device and the sampling device are provided in multiple numbers, and there is a one-to-one correspondence between the multiple sample retention devices and the multiple sampling devices; The refrigeration device is used to cool the sample obtained in the sampling device; The data acquisition and control device is used to obtain the data of the sample to be taken in the sample retention device and the data of the sample obtained in the sampling device, and automatically control the sample retention device, the sampling device and the refrigeration device to cooperate to perform the sampling operation according to the obtained data.

[0007] Further, the sample retention device includes a sliding guide rail, a baffle, a sample box and a first driving device; the sliding guide rail and the baffle are respectively installed on the top of the workbench by bolts, and the plate surface of the baffle is perpendicularly corresponding to the center line of the sliding guide rail; the sliding guide rail is slidably connected to the sample box, the sample box is used to place the sample to be taken, the head end of the sample box is open, the tail end of the sample box is drivingly connected to the first driving device, and the baffle is correspondingly located at the open head end of the sample box; the first driving device is electrically connected to the control pin of the data acquisition and control device.

[0008] Further, the first driving device includes a first gantry bracket, a first driving motor, a first driving electric cylinder, a first transmission frame and a first guide rail. The first gantry bracket is fixedly installed on the top of the workbench, and the first driving motor and the first driving electric cylinder are fixedly installed on the first gantry bracket. Among them, the first driving motor is electrically connected to the control pin of the data acquisition and control device, and the first driving motor is used to drive the first driving electric cylinder to perform telescopic action; the telescopic end of the first driving electric cylinder is fixedly connected to the first transmission frame, the first transmission frame is slidably installed on the first guide rail, and the first transmission frame is fixedly connected to the tail end of the sample box through a first connecting rod.

[0009] Further, a proximity switch is provided on the side surface of the first guide rail, and the proximity switch is used to detect the position of the first transmission frame.

[0010] Further, five sample retention devices are provided, and the five sample retention devices are evenly distributed along the length direction of the first transmission frame.

[0011] Further, the sampling device includes a sampling outer tube, a second connecting rod, a second driving device, a sampling inner tube, a piston oil cylinder and a bottom plate. Among them, the sampling outer tube is located above the sample retention device, the top of the sampling outer tube is connected to the second driving device through a second connecting rod, an inner hole is opened on the bottom surface of the sampling outer tube, and the bottom of the sampling outer tube is fixedly connected to the bottom plate through bolts, and a pressure sensor is installed on the bottom plate; The sampling inner tube is located in the inner hole. The bottom of the sampling inner tube is open and penetrates through the bottom plate. A cooling medium output by a refrigeration device is filled between the outer wall of the sampling inner tube and the inner wall of the sampling outer tube. A piston oil cylinder is installed at the top of the sampling outer tube. The piston end of the piston oil cylinder hermetically penetrates through the sampling outer tube and is fitted inside the sampling inner tube. The pressure sensor is electrically connected to the acquisition pin of the data acquisition and control device. The second driving device and the piston oil cylinder are respectively and correspondingly connected to the control pins of the data acquisition and control device.

[0012] Further, the second driving device includes a second gantry bracket, a second driving motor, a second driving electric cylinder, a second transmission frame and a second guide rail. The second gantry bracket is fixedly installed on the top of the workbench. The second driving motor and the second driving electric cylinder are fixedly installed on the second gantry bracket. The second driving motor is electrically connected to the control pin of the data acquisition and control device and is used to drive the telescopic movement of the second driving electric cylinder. The telescopic end of the second driving electric cylinder is fixedly connected to the second transmission frame. The second transmission frame is slidably installed on the second guide rail and is fixedly connected to the second connecting rod.

[0013] Further, a tube displacement sensor is arranged on the tube wall of the sampling outer tube.

[0014] Further, five sampling devices are provided, and the five sampling devices are evenly distributed along the length direction of the second transmission frame.

[0015] Further, the refrigeration device includes a liquid nitrogen storage tank, a liquid nitrogen refrigeration unit and a liquid nitrogen circulation pump. The liquid nitrogen storage tank, the liquid nitrogen refrigeration unit and the liquid nitrogen circulation pump are all installed inside the workbench. The liquid outlet of the liquid nitrogen storage tank is connected to the liquid nitrogen refrigeration unit through a pipeline. The outlet of the liquid nitrogen refrigeration unit is connected to the liquid nitrogen circulation pump through a pipeline. The liquid nitrogen circulation pump is correspondingly communicated with the inner cavity space between the sampling inner tube and the sampling outer tube through a pipeline. The inner cavity space between the sampling inner tube and the sampling outer tube is communicated with the liquid nitrogen storage tank through a pipeline.

[0016] The technical solution of the present invention has at least the following advantages and beneficial effects: The present invention discloses an automated sterile fecal sampling and freezing storage system suitable for clinical inspection. Through the data acquisition and control device, automated fecal sampling can be realized, the collection efficiency can be improved, human error can be reduced, and the standardization level can be improved. The one-to-one correspondence setting of multiple sample retention devices and multiple sampling devices can avoid sample cross-contamination and improve data reliability. The refrigeration device can realize instant low-temperature freezing preservation to ensure sample stability. And this system helps to solve the technical problems of low standardization degree, cumbersome operation, unstable sample preservation, risk of cross-contamination and environmental pollution in traditional manual fecal sampling. Description of the Drawings

[0017] Figure 1 is a schematic structural view of the present invention; Figure 2 is a schematic structural view of a sample retention device of the present invention; Figure 3 is a schematic structural view of a sample box of the present invention; Figure 4 is a schematic structural view of a sampling device of the present invention; Figure 5 is a schematic structural view of a sampling inner tube of the present invention; Figure 6 is a schematic structural view of a refrigeration device of the present invention.

[0018] Reference numerals: 1, workbench; 2, sample retention device; 21, sliding guide rail; 22, baffle; 23, sample box; 24, first driving device; 241, first gantry bracket; 242, first driving motor; 243, first driving electric cylinder; 244, first transmission frame; 245, first guide rail; 246, first connecting rod; 25, proximity switch; 3, sampling device; 31, sampling outer tube; 32, second connecting rod; 33, second driving device; 331, second gantry bracket; 332, second driving motor; 333, second driving electric cylinder; 334, second transmission frame; 335, second guide rail; 34, sampling inner tube; 35, piston oil cylinder; 36, bolt; 37, bottom plate; 38, pressure sensor; 39, sealing ring; 4, refrigeration device; 41, liquid nitrogen storage tank; 42, liquid nitrogen refrigeration unit; 43, liquid nitrogen circulation pump; 5, data acquisition and control device; 6, pipe displacement sensor; 7, oil supply device; 71, hydraulic oil tank; 72, hydraulic oil pump; 73, electromagnetic reversing valve; 74, oil pipeline. Detailed implementation manners

[0019] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are some, but not all, of the embodiments of the present invention. Usually, the components of the embodiments of the present invention described and illustrated herein can be arranged and designed in various different configurations.

[0020] Embodiment 1 As Figures 1-6 shown, an automated sterile fecal sampling and freezing storage system applicable to clinical examinations includes a workbench 1, and a sampling device 3, a sample retention device 2, a refrigeration device 4, and a data acquisition and control device 5 installed on the workbench 1; The sample retention device 2 is used to place the sample to be taken, which is the fecal sample to be tested; The sampling device 3 is used to obtain the sample to be taken in the sample retention device 2; The refrigeration device 4 is used to cool the sample obtained by the sampling device 3; Both the sample retention device 2 and the sampling device 3 are provided in multiple numbers, and there is a one-to-one correspondence between the multiple sample retention devices 2 and the multiple sampling devices 3; that is, the samples to be taken placed in each sample retention device 2 are independent samples, and the corresponding sampling device 3 will only extract one sample to be taken, which can avoid cross-contamination between samples and improve the reliability of the data obtained in subsequent detections; The data acquisition and control device 5 is used to obtain the data of the sample to be taken in the sample retention device 2 and the data of the sample obtained by the sampling device 3, and automatically control the sample retention device 2, the sampling device 3, and the refrigeration device 4 to cooperate to perform the sampling operation according to the obtained data; Among them, due to the individual differences in the physical properties of different samples to be taken, such as density and viscosity, loose or irregularly shaped samples may cause fluctuations in the sampling volume. Therefore, the sample retention device 2 generally protrudes a part of the sample to be taken, so as to facilitate the sampling operation of the subsequent sampling device 3. Furthermore, the data of the sample to be taken obtained is used to judge whether the protruding height of the sample to be taken is appropriate; the implementation method is the stroke of the first driving cylinder 243 in the first driving device 24, that is, the installation position of the proximity switch 25 is pre-selected. Thus, when the system starts, the first driving motor 242 will start and drive the first driving cylinder 243 to move. When the proximity switch 25 detects that the transmission frame has moved in place, it means that the protruding height of the sample to be taken is already appropriate, and the first driving motor 242 is stopped, thereby defining the stroke of the first driving cylinder 243; In addition, an infrared distance sensor can also be set at a certain distance above the sample retention device 2. When the distance data detected by the infrared distance sensor is a relatively large value, it means that the protruding height of the sample to be taken has not reached. When the distance data detected by the infrared distance sensor becomes a relatively small value, it means that the protruding height of the sample to be taken has reached, then the extrusion of the sample direct current device can be stopped, and the sampling of the sampling device 3 can be started.

[0021] The data acquisition part obtains the sample data obtained in the sampling device 3, including whether the sampling device 3 reaches the position of the sample to be taken and whether the sampling device 3 has completed the sample acquisition. The implementation method is obtained through the pressure sensor 38 and the action stroke of the piston oil cylinder 35 in the sampling device 3. When the pressure sensor 38 in the sampling device 3 detects a certain pressure value, it indicates that the bottom of the sampling device 3 contacts the sample to be taken in the sample retention device 2. At this time, the piston oil cylinder 35 is started, and using the principle of atmospheric pressure, the sample to be taken is left in the sampling inner tube 34. When the action stroke of the piston oil cylinder 35 ends, the refrigeration device 4 is started, and by filling the cooling medium in the cavity between the sampling inner tube 34 and the sampling outer tube 31, the sample in the sampling inner tube 34 is quickly cooled to achieve instant cryopreservation and ensure the stability of the sample.

[0022] Embodiment 2 As an embodiment, the sample retention device 2 includes a sliding guide rail 21, a baffle 22, a sample box 23 and a first driving device 24; the sliding guide rail 21 and the baffle 22 are respectively installed on the top of the workbench 1 by bolts, and the plate surface of the baffle 22 is perpendicular to the center line of the sliding guide rail 21, that is, the baffle 22 and the sliding guide rail 21 are perpendicular to each other in a line-plane relationship; the sliding guide rail 21 is slidably connected to the sample box 23, the sample box 23 is used to place the sample to be taken, the head end of the sample box 23 is open, the tail end of the sample box 23 is drivingly connected to the first driving device 24, the baffle 22 is correspondingly located at the open head end of the sample box 23, and a complete lidless box body is correspondingly formed between the baffle 22 and the sample box 23. When the first driving device 24 drives the sample box 23 to move towards the baffle 22, since the baffle 22 is fixed, the space in the sample box 23 where the sample to be taken can be placed will gradually become smaller, and thus the sample to be taken will be extruded to protrude upward; the first driving device 24 is electrically connected to the control pins of the data acquisition and control device 5; In addition, the first driving device 24 includes a first gantry bracket 241, a first driving motor 242, a first driving electric cylinder 243, a first transmission frame 244 and a first guide rail 245. The first gantry bracket 241 is fixedly installed on the top of the workbench 1, and the first driving motor 242 and the first driving electric cylinder 243 are fixedly installed on the first gantry bracket 241. The first driving motor 242 is electrically connected to the control pins of the data acquisition and control device 5, and the first driving motor 242 is used to drive the first driving electric cylinder 243 to perform telescopic actions; the telescopic end of the first driving electric cylinder 243 is fixedly connected to the first transmission frame 244, the first transmission frame 244 is slidably installed on the first guide rail 245, and the first transmission frame 244 is fixedly connected to the tail end of the sample box 23 through a first connecting rod 246; Specifically, a proximity switch 25 is provided on the side of the first guide rail 245. The proximity switch 25 is used to detect the position of the first transmission frame 244. After the system is started, the first drive motor 242 will be controlled to start. Then, the motor controls the first drive electric cylinder 243 to extend, driving the first transmission frame 244 to move along the first guide rail 245. Thus, the first connecting rod 246 drives the sample box 23 to move along the sliding guide rail 21, and the baffle 22 is used to complete the extrusion of the sample to be taken. When the proximity switch 25 detects that the first transmission frame 244 has moved in place, the first drive motor 242 is stopped, and the extrusion of the sample to be taken is completed. It should be noted that multiple first connecting rods 246 are fixedly arranged on the first transmission frame 244, and each first connecting rod 246 is fixedly connected to the tail end of the sample box 23 of a sampling and retention device 2 respectively. Thus, the first transmission frame 244 can control the movement of multiple sample boxes 23 simultaneously, and the moving distance of each sample box 23 is the same.

[0023] In addition, five sampling and retention devices 2 are provided, and the five sampling and retention devices 2 are evenly distributed along the length direction of the first transmission frame 244.

[0024] Embodiment 3 As an embodiment, the sampling device 3 includes a sampling outer tube 31, a second connecting rod 32, a second drive device 33, a sampling inner tube 34, and a piston oil cylinder 35. The sampling outer tube 31 is located above the sampling and retention device 2. The top of the sampling outer tube 31 is connected to the second drive device 33 through the second connecting rod 32. An inner hole is provided on the bottom surface of the sampling outer tube 31, and the bottom of the sampling outer tube 31 is fixedly connected to the bottom plate 37 through a bolt 36. A pressure sensor 38 is installed on the bottom plate 37. The second drive device 33 is used to drive the overall movement of the sampling outer tube 31 and the sampling inner tube 34, mainly for the action stroke during the sampling process. The sampling inner tube 34 is located in the inner hole. The bottom of the sampling inner tube 34 is open and penetrates the bottom plate 37. A cooling medium output by the cooling device 4 is filled between the outer wall of the sampling inner tube 34 and the inner wall of the sampling outer tube 31. A piston oil cylinder 35 is installed on the top of the sampling outer tube 31. The piston end of the piston oil cylinder 35 hermetically penetrates the sampling outer tube 31 and is fitted inside the sampling inner tube 34. The initial position of the piston end of the piston oil cylinder 35 is at the bottom of the sampling inner tube 34. When the piston oil cylinder 35 is started, the piston end will move towards the top of the sampling inner tube 34. Thus, under the principle of atmospheric pressure, the sample to be taken will enter the sampling inner tube 34, and the sampling operation is completed. When the piston end reaches the top of the sampling inner tube 34, the piston oil cylinder 35 stops operating. Then, the cooling device 4 is started to make the cooling medium enter the inner cavity between the sampling inner tube 34 and the sampling outer tube 31, realizing the cooling of the sample obtained in the sampling inner tube 34. It should be noted that sealing rings 39 are provided between the outer top surface of the sampling inner tube 34 and the inner top surface of the sampling outer tube 31, between the bottom surface of the sampling inner tube 34 and the bottom plate 36, and between the sampling outer tube 31 and the bottom plate 36, for preventing the cooling medium in the inner cavity between the sampling inner tube 34 and the sampling outer tube 31 from flowing out; The pressure sensor 38 is electrically connected to the acquisition pin of the data acquisition and control device 5, and the second driving device 33 and the piston oil cylinder 35 are respectively and correspondingly connected to the control pins of the data acquisition and control device 5; wherein after the pressure sensor 38 contacts the sample to be taken, the detected pressure value thereof will change, so as to be used for judging whether the sampling inner tube 34 can start the sampling operation; and the second driving device 33 is used for driving the sampling outer tube 31 and the sampling inner tube 34 to move in a direction approaching or away from the sample placement device 2, and the action of the piston oil cylinder 35 is used for performing the sampling operation.

[0025] In addition, the second driving device 33 includes a second gantry bracket 331, a second driving motor 332, a second driving electric cylinder 333, a second transmission frame 334 and a second guide rail 335. The second gantry bracket 331 is fixedly installed on the top of the workbench 1, and the second driving motor 332 and the second driving electric cylinder 333 are fixedly installed on the second gantry bracket 331. The second driving motor 332 is electrically connected to the control pin of the data acquisition and control device 5, and is used for driving the second driving electric cylinder 333 to perform telescopic action; the telescopic end of the second driving electric cylinder 333 is fixedly connected to the second transmission frame 334, the second transmission frame 334 is slidably installed on the second guide rail 335, and the second transmission frame 334 is fixedly connected to the installation end of the second connecting rod 32; after the system is started, the second driving motor 332 will be controlled to start, and then the motor controls the second driving electric cylinder 333 to extend, driving the second transmission frame 334 to move along the second guide rail 335. When the detection data of the pressure sensor 38 changes, the second driving motor 332 is stopped, so as to drive a plurality of sampling devices 3 to be located at the sampling station, and the piston oil cylinder 35 is started. During the process of the piston end of the piston oil cylinder 35 retracting, the sample to be taken will gradually enter the sampling inner tube 34. After the piston end of the piston oil cylinder 35 retracts to the top of the sampling inner tube 34, the sampling operation is completed. Immediately afterwards, the refrigeration device 4 is started to enable the cooling medium to enter the inner cavity between the sampling inner tube 34 and the sampling outer tube 31, so as to cool the sample obtained in the sampling inner tube 34; It should be noted that to prevent air from entering the inner tube of the sampling tube during the sampling process and thus contaminating the sampled sample, when the bottom of the sampling device 3 touches the fecal sample, sampling is not immediately performed. After the data acquisition and control device 5 receives the change signal sent by the pressure sensor 38, the action time of the second driving device 33 can be extended to increase the downward displacement of the sampling outer tube 31 and the sampling inner tube 34. When the downward displacement of the sampling outer tube 31 and the sampling inner tube 34 reaches 3 mm, the bottom end of the sampling device 3 completely extends into the sample to be taken. At this time, sampling is performed, and air will not be mixed into the sampling inner tube 34. And immediately cooling the sample after sampling is completed also makes the obtained sample a sterile sample, thus ensuring the accuracy of the subsequent sample test results.

[0026] Specifically, a tube displacement sensor 6 is provided on the tube wall of the sampling outer tube 31. When the data acquisition and control device 5 receives the change signal sent by the pressure sensor 38, the tube displacement sensor 6 starts to record the moving distance of the sampling outer tube 31. When the downward displacement of the sampling outer tube 31 and the sampling inner tube 34 reaches 3 mm, the bottom end of the sampling device 3 completely extends into the sample to be taken. At this time, sampling is performed, and air will not be mixed into the sampling inner tube 34. In addition, since each sampling device 3 is provided with a piston oil cylinder 35, each sampling device 3 operates independently, improving the operability of the sampling system. Moreover, the spatial positions of the second gantry bracket 331 and the first gantry bracket 241 are in a corresponding vertical relationship.

[0027] As required, five sampling devices 3 are provided, and the five sampling devices 3 are evenly distributed along the length direction of the second transmission frame 334.

[0028] In addition, the piston oil cylinder 35 in the sampling device 3 realizes linear motion by using the pressure of hydraulic oil. Therefore, a set of oil supply device 7 is also installed in the workbench 1 to complete the drive of the piston oil cylinder 35. The oil supply device includes a hydraulic oil tank 71, a hydraulic oil pump 72, and an electromagnetic reversing valve 73. The hydraulic oil tank 71 and the hydraulic oil pump 72 are placed on the inner bottom surface of the workbench 1, and the hydraulic oil tank 71, the hydraulic oil pump 72, the electromagnetic reversing valve 73, and the piston oil cylinder 35 are sequentially connected through an oil pipeline 74.

[0029] Embodiment 4 As an embodiment, the refrigeration device 4 includes a liquid nitrogen storage tank 41, a liquid nitrogen refrigeration unit 42, and a liquid nitrogen circulation pump 43. The liquid nitrogen storage tank 41, the liquid nitrogen refrigeration unit 42, and the liquid nitrogen circulation pump 43 are all installed inside the workbench 1. The liquid outlet of the liquid nitrogen storage tank 41 is connected to the liquid nitrogen refrigeration unit 42 through a pipeline. The outlet of the liquid nitrogen refrigeration unit 42 is connected to the liquid nitrogen circulation pump 43 through a pipeline. The liquid nitrogen circulation pump 43 is correspondingly connected in communication with the inner cavity space between the sampling inner tube 34 and the sampling outer tube 31 through a pipeline. The inner cavity space between the sampling inner tube 34 and the sampling outer tube 31 is connected to the liquid nitrogen storage tank 41 through a pipeline; That is, the cooling medium output by the refrigeration device 4 is liquid nitrogen, and its temperature can reach -80°C. The liquid nitrogen refrigeration unit 42 is used to cool the liquid nitrogen after leaving the liquid nitrogen storage tank 41 so that it can be maintained at a temperature of -80°C. The liquid nitrogen circulation pump 43 is used to let the liquid nitrogen return to the liquid nitrogen storage tank 41 after passing through the inner cavity between the sampling inner tube 34 and the sampling outer tube 31, use the liquid nitrogen to cool the sample in the sampling inner tube 34, and recycle the liquid nitrogen.

[0030] Embodiment 5 The data acquisition and control device 5 is composed of a PLC and an interactive display screen. The acquisition input pins of the PLC are respectively electrically connected to the proximity switch 25, the pressure sensor 38, the pipe displacement sensor 6, and the piston oil cylinder 35. The control output pins of the PLC are respectively electrically connected to the first drive motor 242, the second drive motor 332, and the piston oil cylinder 35. The data interaction pin of the PLC is electrically connected to the interactive display screen; A control program for automatically controlling the actions of the sample retention device 2, the sampling device 3, and the refrigeration device 4 is written inside the PLC. The data of the proximity switch 25, the pressure sensor 38, the pipe displacement sensor 6, and the piston oil cylinder 35 are respectively judgment nodes in the control program. That is, when the proximity switch 25 detects that the first transmission frame 244 is in place and sends a signal, when the data detected by the pressure sensor 38 changes, after the data detected by the pressure sensor 38 changes, the pipe displacement sensor 6 detects that the sampling outer tube 31 has displaced another 3 millimeters, and when the piston oil cylinder 35 contracts to the maximum stroke; then the control program will perform corresponding controls on the first drive motor 242, the second drive motor 332, and the piston oil cylinder 35 according to these judgment nodes. Therefore, the actions of the first drive motor 242, the second drive motor 332, and the piston oil cylinder 35 are also control nodes; thus realizing the automated operation of fecal sampling and frozen storage; The interactive display screen has the functions of human-computer interaction and display. The display function is to display some control nodes of the control program in a combination of graphics and text. The human-computer interaction function is that when a staff member selects a certain control node on the interactive display screen, the action of the control node in the control program can be changed, thereby quickly adjusting the automatic operation process of the system.

[0031] The above are only the preferred embodiments of the present invention and are not intended to limit the present invention. For those skilled in the art, the present invention may have various changes and modifications. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included within the protection scope of the present invention.

Claims

1. An automated sterile fecal sampling and cryogenic storage system applicable to clinical examinations, characterized in that, It includes a workbench (1), as well as a sample retention device (2), a sampling device (3), a refrigeration device (4), and a data acquisition and control device (5) installed on the workbench (1); The sample retention device (2) is used to place the sample to be taken; The sampling device (3) is used to obtain the sample to be taken in the sample retention device (2); Both the sample retention device (2) and the sampling device (3) are provided in multiple numbers, and there is a one-to-one correspondence between the multiple sample retention devices (2) and the multiple sampling devices (3); The refrigeration device (4) is used to cool the sample obtained in the sampling device (3); The data acquisition and control device (5) is used to obtain the data of the sample to be taken in the sample retention device (2) and the data of the sample obtained in the sampling device (3), and automatically control the sample retention device (2), the sampling device (3), and the refrigeration device (4) to cooperate to perform the sampling operation according to the acquired data.

2. The automated sterile fecal sampling and cryogenic storage system applicable to clinical tests according to claim 1, characterized in that: The sample retention device (2) includes a sliding guide rail (21), a baffle (22), a sample box (23), and a first driving device (24); the sliding guide rail (21) and the baffle (22) are respectively installed on the top of the workbench (1) by bolts, and the plate surface of the baffle (22) is perpendicularly corresponding to the center line of the sliding guide rail (21); the sliding guide rail (21) is slidably connected to the sample box (23), the sample box (23) is used to place the sample to be taken, the head end of the sample box (23) is open, the tail end of the sample box (23) is drivingly connected to the first driving device (24), and the baffle (22) is correspondingly located at the open head end of the sample box (23); the first driving device (24) is electrically connected to the control pin of the data acquisition and control device (5).

3. The automated aseptic fecal sampling and cryogenic storage system applicable to clinical tests according to claim 2, wherein: The first driving device (24) includes a first gantry bracket (241), a first driving motor (242), a first driving electric cylinder (243), a first transmission frame (244), and a first guide rail (245). The first gantry bracket (241) is fixedly installed on the top of the workbench (1), and the first driving motor (242) and the first driving electric cylinder (243) are fixedly installed on the first gantry bracket (241). Among them, the first driving motor (242) is electrically connected to the control pin of the data acquisition and control device (5), and this first driving motor (242) is used to drive the first driving electric cylinder (243) to perform telescopic movement; the telescopic end of the first driving electric cylinder (243) is fixedly connected to the first transmission frame (244), the first transmission frame (244) is slidably installed on the first guide rail (245), and the first transmission frame (244) is fixedly connected to the tail end of the sample box (23) through a first connecting rod (246).

4. The automated sterile fecal sampling and cryogenic storage system applicable to clinical tests according to claim 3, characterized in that: A proximity switch (25) is provided on the side of the first guide rail (245), and the proximity switch (25) is used to detect the position of the first transmission frame (244).

5. The automated sterile fecal sampling and cryogenic storage system applicable to clinical tests according to claim 3, characterized in that: The sample retention device (2) is provided in five numbers, and the five sample retention devices (2) are evenly distributed along the length direction of the first transmission frame (244).

6. The automated sterile fecal sampling and cryogenic storage system applicable to clinical examinations according to claim 1, wherein: The sampling device (3) includes a sampling outer tube (31), a second connecting rod (32), a second driving device (33), a sampling inner tube (34) and a piston oil cylinder (35). The sampling outer tube (31) is located above the sample retention device (2). The top of the sampling outer tube (31) is connected to the second driving device (33) through the second connecting rod (32). An inner hole is provided on the bottom surface of the sampling outer tube (31), and the bottom of the sampling outer tube (31) is fixedly connected to the bottom plate (37) through bolts (36). A pressure sensor (38) is installed on the bottom plate (37). The sampling inner tube (34) is located in the inner hole. The bottom of the sampling inner tube (34) is open and penetrates through the bottom plate (37). A cooling medium output by the refrigeration device (4) is filled between the outer wall of the sampling inner tube (34) and the inner wall of the sampling outer tube (31). A piston oil cylinder (35) is installed at the top of the sampling outer tube (31). The piston end of the piston oil cylinder (35) hermetically penetrates through the sampling outer tube (31) and is fitted inside the sampling inner tube (34). The pressure sensor (38) is electrically connected to the acquisition pin of the data acquisition and control device (5). The second driving device (33) and the piston oil cylinder (35) are respectively connected to the control pins of the data acquisition and control device (5).

7. The automated aseptic fecal sampling and cryogenic storage system applicable to clinical tests according to claim 6, wherein: The second driving device (33) includes a second gantry bracket (331), a second driving motor (332), a second driving electric cylinder (333), a second transmission frame (334) and a second guide rail (335). The second gantry bracket (331) is fixedly installed on the top of the workbench (1). The second driving motor (332) and the second driving electric cylinder (333) are fixedly installed on the second gantry bracket (331). The second driving motor (332) is electrically connected to the control pin of the data acquisition and control device (5) and is used to drive the telescopic movement of the second driving electric cylinder (333). The telescopic end of the second driving electric cylinder (333) is fixedly connected to the second transmission frame (334). The second transmission frame (334) is slidably installed on the second guide rail (335), and the second transmission frame (334) is fixedly connected to the second connecting rod (32).

8. The automated aseptic fecal sampling and cryogenic storage system applicable to clinical tests according to claim 7, wherein: A tube displacement sensor (6) is provided on the tube wall of the sampling outer tube (31).

9. The automated sterile fecal sampling and cryogenic storage system applicable to clinical examinations according to claim 7, characterized in that: There are five sampling devices (3), and the five sampling devices (3) are evenly distributed along the length direction of the second transmission frame (334).

10. The automated sterile fecal sampling and cryogenic storage system applicable to clinical tests according to claim 6, wherein: The refrigeration device (4) includes a liquid nitrogen storage tank (41), a liquid nitrogen refrigeration unit (42) and a liquid nitrogen circulation pump (43). The liquid nitrogen storage tank (41), the liquid nitrogen refrigeration unit (42) and the liquid nitrogen circulation pump (43) are all installed inside the workbench (1). The liquid outlet of the liquid nitrogen storage tank (41) is connected to the liquid nitrogen refrigeration unit (42) through a pipeline. The outlet of the liquid nitrogen refrigeration unit (42) is connected to the liquid nitrogen circulation pump (43) through a pipeline. The liquid nitrogen circulation pump (43) is correspondingly connected to the inner cavity space between the sampling inner tube (34) and the sampling outer tube (31) through a pipeline. The inner cavity space between the sampling inner tube (34) and the sampling outer tube (31) is connected to the liquid nitrogen storage tank (41) through a pipeline.

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