Traditional Chinese medicine composition for resisting gastric cancer as well as preparation method and application of traditional Chinese medicine composition

Abstract

The invention provides a traditional Chinese medicine composition for resisting gastric cancer. Compared with the prior art, the dioscorea panthaica in the traditional Chinese medicine composition provided by the invention has the effects of regulating qi, relieving pain, detoxifying and diminishing swelling and is a monarch drug of the traditional Chinese medicine composition, the frankincense and the myrrh have the effects of activating blood, relieving pain, diminishing swelling and promoting tissue regeneration, the mangnolia officinalis has the effects of eliminating dampness, dissolving phlegm, descending qi and removing fullness, the semen aesculi has the effects of soothing liver, regulating qi, harmonizing stomach and relieving pain, and the four drugs are used as ministerial drugs and have the effects of eliminating dampness, eliminating phlegm and relieving pain. The alpinia katsumadai has the effects of eliminating dampness, promoting the circulation of qi and warming middle and arresting vomiting, the rhizoma nardostachyos has the effects of regulating qi, relieving pain, relieving depression and enlivening the spleen and can improve the symptoms such as hematemesis, stomachache, warm qi, adverse flow of vomiting, inappetence and the like, and the albizia flowers have the effects of resolving depression and soothing the nerves and can relieve the emotional symptoms such as unease, depression and insomnia of patients and serve as conductant drugs. The traditional Chinese medicine composition has the effects of regulating qi to alleviate pain, removing toxicity for detumescence, promoting blood circulation to remove blood stasis, eliminating dampness and tonifying spleen and is suitable for gastric cancer patients and gastric cancer postoperative recovery periods.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition for treating gastric cancer, a preparation method thereof, and an application thereof. Background Art

[0002] Worldwide, gastric cancer is the fifth most common cancer, and its related mortality rate ranks third after lung cancer and liver cancer.

[0003] The main treatment methods for gastric cancer are chemotherapy and surgery. However, most radical gastrectomies involve partial gastrectomy, and the postoperative trauma will inevitably inhibit normal gastrointestinal motility and digestive function, further reducing the body's immune function of the patient, making it easy for residual tumors to grow and metastasize and for various complications to occur, thus affecting the short-term recovery and long-term survival rate. Therefore, great attention should be paid to the early symptoms of gastric cancer to avoid delaying diagnosis and treatment. Moreover, the mortality rate of gastric cancer shows an increasing trend year by year, and it is expected that the incidence of gastric cancer will continue to rise in the next few years, and the prevention and treatment situation is severe.

[0004] Traditional Chinese medicine is a treasure of the motherland's medicine. Originating from the ancient Chinese cultural tradition and developing over thousands of years, it has formed a unique theoretical system and treatment methods, and has achieved good results in the prevention and treatment of gastric cancer. Compared with traditional Western medicine treatment methods, traditional Chinese medicine usually has lower side effects and better individual adaptability, and can meet the needs of different patients. In the prevention and treatment of gastric cancer, traditional Chinese medicine pays attention to overall conditioning and individualized treatment, emphasizing the balance of functions such as yin and yang, qi and blood in the human body. Traditional Chinese medicine believes that gastric cancer is related to the imbalance of yin and yang in the body, and using traditional Chinese medicine to regulate the balance of yin and yang in the body can play a certain role in prevention and treatment. In addition, traditional Chinese medicine also pays attention to comprehensive therapies, combining traditional Chinese medicine with other treatment methods such as surgery, chemotherapy, radiotherapy, etc. to improve the curative effect. Supplementary traditional Chinese medicine treatment after surgery can not only promote the early recovery of gastrointestinal function and improve immune function, help reduce the occurrence of postoperative complications, but also help prevent tumor recurrence. However, although there are many existing traditional Chinese medicine prescriptions for preventing and treating gastric cancer, their curative effects are relatively poor. Summary of the Invention

[0005] In view of this, the technical problem to be solved by the present invention is to provide a traditional Chinese medicine composition for treating gastric cancer, a preparation method thereof, and an application thereof. The traditional Chinese medicine composition has the advantage of good treatment effect on gastric cancer, and is expected to be applied to the treatment or adjuvant treatment of gastric cancer.

[0006] The present invention provides a traditional Chinese medicine composition for treating gastric cancer, comprising:

[0007]

[0008] Preferably, it comprises:

[0009]

[0010] The present invention also provides a preparation method of the above-mentioned traditional Chinese medicine composition for anti-gastric cancer, comprising the following steps:

[0011] Mix Dioscorea nipponica Makino, frankincense, myrrh, dalbergia odorifera, magnolia officinalis, reevesia rotundifolia, nardostachys jatamansi, tsaoko fruit and albizia julibrissin and extract with alcohol to obtain the traditional Chinese medicine composition for anti-gastric cancer.

[0012] Preferably, the alcohol is an ethanol aqueous solution of 80% - 95%; the extraction is Soxhlet extraction.

[0013] Preferably, the volume ratio of the total mass of the Chinese yam, frankincense, myrrh, dalbergia odorifera, magnolia officinalis, reevesia rotundifolia, nardostachys jatamansi, tsaoko fruit and albizia julibrissin to the alcohol is 1 g:(10 - 20) mL.

[0014] Preferably, after extraction, concentrate and freeze-dry to obtain the traditional Chinese medicine composition for anti-gastric cancer.

[0015] Preferably, the temperature of concentration is 50°C - 80°C; the concentration ratio is 8 - 12.

[0016] Preferably, the temperature of freeze-drying is -50°C - -110°C; the pressure of freeze-drying is 10 - 100 Pa.

[0017] The present invention also provides an application of the above-mentioned traditional Chinese medicine composition in the preparation of a medicament for treating and / or preventing gastric cancer.

[0018] The present invention provides a traditional Chinese medicine composition for anti-gastric cancer, comprising: 15 - 30 parts by weight of Dioscorea nipponica Makino; 3 - 5 parts by weight of frankincense; 3 - 5 parts by weight of myrrh; 5 - 15 parts by weight of dalbergia odorifera; 5 - 10 parts by weight of magnolia officinalis; 3 - 9 parts by weight of reevesia rotundifolia; 3 - 6 parts by weight of nardostachys jatamansi; 3 - 6 parts by weight of tsaoko fruit; 5 - 10 parts by weight of albizia julibrissin. Compared with the prior art, in the traditional Chinese medicine composition provided by the present invention, Dioscorea nipponica Makino has the functions of regulating qi to relieve pain and detoxifying and detumescence, and can soothe the discomfort such as stomachache and abdominal pain caused by gastric cancer, and is the monarch drug of this formula. Frankincense and myrrh both have the functions of promoting blood circulation to stop pain and promoting tissue regeneration to relieve swelling, and can relieve symptoms such as chest pain and epigastric pain. Magnolia officinalis has the effects of drying dampness and resolving phlegm, and lowering qi to remove fullness, and is applicable to symptoms such as dampness stagnating in the middle energizer, abdominal distension and constipation. Reevesia rotundifolia soothes the liver and regulates qi, and harmonizes the stomach to relieve pain, and can relieve symptoms such as liver-stomach qi stagnation and chest and abdominal distension. These four herbs are the ministerial drugs in total. Dalbergia odorifera is used as the assistant drug for promoting blood circulation to remove stasis and stop bleeding, regulating qi to relieve pain. Tsao-ko fruit dries dampness and promotes qi movement, warms the stomach to arrest vomiting. Nardostachys jatamansi has the functions of regulating qi to relieve pain and promoting qi circulation and awakening the spleen, and can improve symptoms such as hematemesis, abdominal pain, belching and vomiting, and loss of appetite. Albizia julibrissin has the effect of relieving depression and calming the mind, and relieves the emotional symptoms such as restlessness of mind and melancholy and insomnia of patients, and is the adjuvant drug. The combination of all herbs has the effects of regulating qi to relieve pain, detoxifying and detumescence, promoting blood circulation to remove stasis, drying dampness and strengthening the spleen, and is applicable to gastric cancer patients and the recovery period after gastric cancer surgery. Description of the Drawings

[0019] Figure 1 This is the result diagram of the inhibitory effect of the traditional Chinese medicine composition prepared in Examples 1 to 3 of the present invention on human gastric cancer MKN45 cells;

[0020] Figure 2 This is the result diagram of the inhibitory effect of the traditional Chinese medicine composition prepared in Example 2 and Comparative Examples 1 to 4 of the present invention on human gastric cancer MKN45 cells. Detailed implementation manners

[0021] Next, in combination with the embodiments of the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0022] The present invention provides a traditional Chinese medicine composition for treating gastric cancer, including:

[0023]

[0024] The dosage form of the traditional Chinese medicine composition provided by the present invention can be the dosage forms prepared from the above nine raw materials well-known to those skilled in the art, without special limitations. Specifically, it includes but is not limited to oral decoctions, tablets, pills, etc. Correspondingly, the traditional Chinese medicine composition can be applied to the treatment or adjuvant treatment of gastric cancer through different administration routes such as oral administration and injection.

[0025] In the present invention, the mass ratio of Dioscorea panthaica to frankincense is preferably (3 - 6):1; in the embodiments provided by the present invention, the mass ratio of Dioscorea panthaica to frankincense is specifically 5:1, 3:1 or 6:1.

[0026] In the present invention, the mass ratio of Dioscorea panthaica to dalbergiawood is preferably (1.6 - 3):1, more preferably (2 - 3):1.

[0027] In the present invention, the mass ratio of Dioscorea panthaica to magnolia officinalis is preferably (1.5 - 6):1, and more preferably (3 - 6):1.

[0028] In the present invention, the mass ratio of Dioscorea panthaica to Chinese sweetgum fruit is preferably (1.6 - 10):1, and more preferably (2.5 - 10):1.

[0029] In the present invention, the mass ratio of Dioscorea panthaica to nardostachys jatamansi is preferably (2.5 - 10):1.

[0030] In the present invention, the mass ratio of Dioscorea panthaica to albizia flower is preferably (1.5 - 6):1, and more preferably (3 - 6):1.

[0031] In the present invention, the content of Dioscorea bulbifera L. in the traditional Chinese medicine composition may specifically be 15 parts by weight or 30 parts by weight.

[0032] In the present invention, the content of frankincense in the traditional Chinese medicine composition may specifically be 3 parts by weight or 5 parts by weight.

[0033] In the present invention, the content of myrrh in the traditional Chinese medicine composition may specifically be 3 parts by weight or 5 parts by weight.

[0034] In the present invention, the content of dalbergia odorifera in the traditional Chinese medicine composition may specifically be 9 parts by weight, 5 parts by weight or 15 parts by weight.

[0035] In the present invention, the content of magnolia officinalis in the traditional Chinese medicine composition may specifically be 10 parts by weight or 5 parts by weight.

[0036] In the present invention, the content of semen aesculi in the traditional Chinese medicine composition may specifically be 9 parts by weight, 6 parts by weight or 3 parts by weight.

[0037] In the present invention, the content of nardostachys jatamansi in the traditional Chinese medicine composition may specifically be 6 parts by weight or 3 parts by weight.

[0038] In the present invention, the content of semen alpina in the traditional Chinese medicine composition may specifically be 6 parts by weight or 3 parts by weight.

[0039] In the present invention, the content of albizia flower in the traditional Chinese medicine composition may specifically be 10 parts by weight or 5 parts by weight.

[0040] In the present invention, more specifically, the traditional Chinese medicine composition for treating gastric cancer includes:

[0041]

[0042]

[0043] Or, it includes:

[0044]

[0045] Or, it includes:

[0046]

[0047] In the traditional Chinese medicine composition provided by the present invention, Dioscorea panthaica has the effects of regulating qi to relieve pain and detoxifying and detumescence, and can soothe the discomfort such as stomachache and abdominal pain caused by gastric cancer, and is the monarch drug of this formula. Both frankincense and myrrh have the effects of promoting blood circulation to stop pain and detumescence and promoting granulation, and can relieve symptoms such as angina pectoris and epigastric pain. Magnolia officinalis has the effects of drying dampness and resolving phlegm, and lowering qi to relieve fullness, and is applicable to symptoms such as dampness stagnating in the middle energizer, abdominal distension and constipation. Chinese sweetgum fruit soothes the liver and regulates qi, and harmonizes the stomach to relieve pain, and can relieve symptoms such as liver-stomach qi stagnation and chest and abdominal distension and stuffiness. These four herbs together serve as the ministerial drugs. The compatibility is supplemented by dalbergia odorifera for promoting blood circulation to remove stasis and stop bleeding, and regulating qi to relieve pain, tsaoko amomum fruit for drying dampness and promoting qi movement, and warming the middle energizer to stop vomiting, and nardostachys jatamansi for regulating qi to relieve pain and opening depression and waking the spleen, which can improve symptoms such as hematemesis, abdominal pain, belching and vomiting, and loss of appetite. Albizia julibrissin has the effect of relieving depression and tranquilizing the mind, and relieves the emotional symptoms such as restlessness and melancholy insomnia of the patient, and is the adjuvant drug. The combination of all the herbs plays the combined effects of regulating qi to relieve pain, detoxifying and detumescence, promoting blood circulation to remove stasis, and drying dampness and strengthening the spleen, and is applicable to gastric cancer patients and the recovery period after gastric cancer surgery.

[0048] The present invention also provides a preparation method of the above anti-gastric cancer traditional Chinese medicine composition, comprising the following steps: mixing Dioscorea panthaica, frankincense, myrrh, dalbergia odorifera, Magnolia officinalis, Chinese sweetgum fruit, nardostachys jatamansi, tsaoko amomum fruit and Albizia julibrissin and extracting with alcohol to obtain the anti-gastric cancer traditional Chinese medicine composition.

[0049] Among them, the present invention has no special restrictions on the sources of all raw materials, and they can be commercially available.

[0050] Mix Dioscorea panthaica, frankincense, myrrh, dalbergia odorifera, Magnolia officinalis, Chinese sweetgum fruit, nardostachys jatamansi, tsaoko amomum fruit and Albizia julibrissin and extract with alcohol; the alcohol is preferably an aqueous ethanol solution, more preferably an aqueous ethanol solution of 80% - 95%, still more preferably an aqueous ethanol solution of 85% - 95%, and most preferably an aqueous ethanol solution of 90%; the total mass ratio of Dioscorea panthaica, frankincense, myrrh, dalbergia odorifera, Magnolia officinalis, Chinese sweetgum fruit, nardostachys jatamansi, tsaoko amomum fruit and Albizia julibrissin to the volume of alcohol is preferably 1 g:(10 - 20) mL, more preferably 1 g:(12 - 18) mL, still more preferably 1 g:(14 - 16) mL, and most preferably 1 g:15 mL; the extraction method can be the method well-known to those skilled in the art without special restrictions, and in the present invention, Soxhlet extraction is preferably used.

[0051] After extraction, it is preferably concentrated and freeze-dried to obtain a traditional Chinese medicine composition against gastric cancer; the temperature of the concentration is preferably 50°C to 80°C, more preferably 50°C to 70°C, and still more preferably 56°C to 60°C; the concentration is preferably concentrated to a concentration ratio of 8 to 12, more preferably to a concentration ratio of 9 to 11, and still more preferably to a concentration ratio of 10; the temperature of the freeze-drying is preferably -50°C to -110°C, more preferably -50°C to -100°C, still more preferably -50°C to -80°C, and most preferably -60°C; the pressure of the freeze-drying is preferably 5 to 100 Pa, more preferably 5 to 80 Pa, still more preferably 5 to 50 Pa, still more preferably 5 to 20 Pa, and most preferably 5 to 10 Pa.

[0052] The present invention also provides an application of the above traditional Chinese medicine composition in the preparation of a drug for treating and / or preventing gastric cancer.

[0053] The traditional Chinese medicine composition provided by the present invention can inhibit the proliferation of human gastric cancer MKN45 cells, prevent their growth, relieve symptoms, and is used for the treatment and adjuvant treatment of gastric cancer.

[0054] In order to further illustrate the present invention, the following is a detailed description of a traditional Chinese medicine composition against gastric cancer, its preparation method and application provided by the present invention in combination with examples.

[0055] The reagents used in the following examples are all commercially available.

[0056] Examples 1 to 4

[0057] Step 1

[0058] Dioscorea panthaica, frankincense, myrrh, dalbergia odorifera, magnolia officinalis, reevesia rotundifolia, nardostachys jatamansi, tsaoko fruit, albizia julibrissin in the formula were respectively pulverized into 90-mesh powders, 10 g of a mixture of medicinal herb powders was weighed in accordance with the ratios in Table 1, 150 ml of 90% ethanol aqueous solution was added, and standard Soxhlet extraction was carried out for 3 h. The extract was concentrated in an oven at 56°C for 20 min to a volume of 2 mL, and the concentration ratio was 10. The concentrated solution was transferred to a freeze dryer for freeze-drying under reduced pressure for 24 h to prepare an extract freeze-dried powder. The freeze-drying temperature was -60°C and the pressure was 6 Pa.

[0059] Table 1 Ratios of raw materials in Examples 1 to 4

[0060]

[0061] II. Inhibitory effects of traditional Chinese medicine composition extracts with different ratios on human gastric cancer MKN45 cells

[0062] 1. The medicinal herb powders in Step 1 were formulated into 3 prescriptions according to different ratios, and were prepared according to the preparation method of the traditional Chinese medicine extract in Step 1 to prepare an extract freeze-dried powder, which was dissolved in DMSO to obtain 3 50 mg / ml traditional Chinese medicine extract mother liquors.

[0063] 2. Seed 10,000 MKN45 cells per well in a 96-well plate and culture them in an incubator at 37 °C and 5% CO2 concentration for 24 h. The culture system is 89% RPMI-1640 (Hyclone, SH30809.01B), 10% FBS (ExcellBio, FND500), and 1% Penicillin-Streptomycin Solution (Hyclone, SV30010), and the volume of the culture medium is 100 μL per well.

[0064] 3. Add the three Chinese medicine extract stock solutions prepared in step 1 to the cells respectively (0.1 μL per well for Examples 1-3, i.e., the final concentration is 50 μg / mL), and add 0.1 μL of DMSO to the control group. A total of 4 groups are cultured at 37 °C for 48 h.

[0065] 4. After culturing the cells with the drugs for about 48 h, take out the cell culture plate from the incubator, use a multi-channel pipette to remove the supernatant, add fresh RPMI1640 medium containing 10% FBS (100 μL per well in the 96-well plate), then use a multi-channel pipette to add CCK8, 10 μL per well, and gently pipette 3 times to mix it with the medium.

[0066] 5. Put the culture plate back into the incubator and continue to culture for 1.5 h, then measure the OD value at 450 nm. (To ensure stable and consistent data, the readings must be taken more than 3 times.)

[0067] 6. Data processing: Calculate the cell survival rate of each group according to the absorbance value. The calculation formula is as follows: Cell survival rate = [(experimental well - blank well) / (control well - blank well)] * 100%, where the blank well refers to the well only added with RPMI1640 medium containing 10% FBS.

[0068] The experimental results were repeated 3 times, and the experimental results are shown in Table 2 and Figure 1 and. As shown by the CCK8 results in Table 2 and Figure 1 , the Chinese medicine compositions with different ratios can inhibit the proliferation of human gastric cancer MKN45 cells to varying degrees (compared with DMSO, **P < 0.01), and Example 3 has the best effect.

[0069] Table 2 CCK8 survival rate results of different ratios of Chinese medicine prescriptions

[0070]

[0071] III. Inhibitory effect of the Chinese medicine composition on human gastric cancer MKN45 cells after drug withdrawal

[0072] 1. Prepare 5 prescriptions with the Chinese herbal medicine pieces powder described in Step 1. Among them, Example 2 is a complete Chinese medicine prescription, and the ratio is as described above. Comparative Examples 1 - 3 are prescription - lacking groups (that is, remove Dioscorea panthaica or Albizia julibrissin or both from the complete Chinese medicine prescription group), and Comparative Example 4 is a prescription - replaced group (that is, replace Dioscorea panthaica and Albizia julibrissin in the prescription of the complete Chinese medicine prescription group with an equal amount of Pueraria lobata and Panax ginseng).

[0073] 2. Seed 10,000 MKN45 cells per well in a 96 - well plate and culture them in an incubator at 37°C and 5% CO₂ concentration for 24 h. The culture system is 89% RPMI - 1640 (Hyclone, SH30809.01B), 10% FBS (Excell Bio, FND500), and 1% Penicillin - Streptomycin Solution (Hyclone, SV30010).

[0074] 3. Add Brucea javanica oil (China Resources Sanjiu Pharmaceutical Co., Ltd.), and the 4 Chinese medicine mother liquors of Example 2 and Comparative Examples 1 - 3 into the cells respectively. The final concentration of each drug is 25 μg / ml, and the final concentration of Brucea javanica oil is 40 μg / ml; add an equal volume of DMSO to the control group. A total of 6 groups are cultured at 37°C for 48 h.

[0075] 4. After culturing the cells for about 48 h after adding the drugs, take out the cell culture plate from the incubator, use a multi - channel pipette to remove the supernatant, add fresh RPMI1640 medium containing 10% FBS (100 μL per well in the 96 - well plate), and then use a multi - channel pipette to add CCK8, 10 μL per well, and gently pipette 3 times to mix it with the medium.

[0076] 5. Put the culture plate back into the incubator and continue to culture for 1.5 h, then measure the OD value at 450 nm. (To ensure the stability and consistency of the data, the reading must be done more than 3 times.)

[0077] 6. Data processing: Calculate the cell survival rate of each group according to the absorbance value. The calculation formula is as follows: Cell survival rate = [(experimental well - blank well) / (control well - blank well)] * 100%, where the blank well refers to the well that only adds RPMI1640 medium containing 10% FBS.

[0078] The experimental results are repeated 3 times, and the experimental results are shown in Table 3 and Figure 2 . From the CCK8 results in Table 3 and Figure 2 , it shows that the positive drug Brucea javanica oil has no obvious inhibitory effect at 40 μg / ml, while Example 2 (complete Chinese medicine prescription) can significantly inhibit the proliferation of human gastric cancer MKN45 cells at a concentration of 25 μg / ml (compared with the DMSO group, **P < 0.01), and the effect is better than that of the positive control drug (compared with Brucea javanica oil, ##P < 0.01). Comparative Example 2 (lacking Albizia julibrissin) and Comparative Example 3 (lacking Dioscorea panthaica and Albizia julibrissin) had no significant inhibitory effect on human gastric cancer MKN45 cells at a concentration of 25 μg / ml, indicating that Albizia julibrissin is indispensable in the formula. In addition, the inhibitory effect of Example 2 (complete traditional Chinese medicine formula) was better than that of Comparative Example 1 (lacking Dioscorea panthaica) (&P < 0.05), further indicating that Dioscorea panthaica is also indispensable in the formula. Comparative Example 4 (replacing Dioscorea panthaica and Albizia julibrissin with Pueraria lobata and Panax ginseng) had no significant inhibitory effect on human gastric cancer MKN45 cells at a concentration of 25 μg / ml, indicating that the lack of inhibitory effect in Comparative Examples 1-3 was not due to a reduction in effective drugs, but rather that Albizia julibrissin and Dioscorea panthaica are indispensable for exerting the drug effect. Note: Compared with the DMSO group, **P < 0.01, ns indicates p > 0.05; compared with the Brucea javanica oil group, ## P < 0.01; compared with the Formula 1 group, &P < 0.05.

[0079] Table 3 CCK8 results of traditional Chinese medicine formulas and Brucea javanica oil

[0080]

[0081]

[0082] The preferred embodiments of the present invention disclosed above are only used to help illustrate the present invention. However, the present invention is not limited thereto. Those skilled in the art can understand that within the scope of the technical concept of the present invention, the technical solutions of the present invention can be modified, or some technical features can be combined in any other way. These modifications or combinations do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of each embodiment of the present invention, and should be regarded as the content disclosed by the present invention and all fall within the protection scope of the present invention.

Claims

1. A traditional Chinese medicine composition for anti-gastric cancer, characterized in that, Comprising:

2. The traditional Chinese medicine composition according to claim 1, characterized in that Including:

3. A preparation method of the traditional Chinese medicine composition for anti-gastric cancer according to claim 1, characterized in that, Comprising the following steps: Mixing Dioscorea opposita Thunb., Olibanum, Myrrha, Dalbergia odorifera T. Chen, Magnolia officinalis Rehd. et Wils., Reevesia rotundifolia, Nardostachys jatamansi DC., Alpinia katsumadai Hayata and Albizia julibrissin Durazz. and extracting with alcohol to obtain a traditional Chinese medicine composition for treating gastric cancer.

4. The preparation method according to claim 3, wherein, The alcohol is an aqueous solution of ethanol at 80% - 95%; the extraction is Soxhlet extraction.

5. The preparation method according to claim 3, characterized in that, The volume ratio of the total mass of Dioscorea opposita Thunb., Olibanum, Myrrha, Dalbergia odorifera T. Chen, Magnolia officinalis Rehd. et Wils., Reevesia rotundifolia, Nardostachys jatamansi DC., Alpinia katsumadai Hayata and Albizia julibrissin Durazz. to the alcohol is 1 g:(10 - 20) mL.

6. The preparation method according to claim 3, characterized in that, After extraction, concentrating and freeze-drying to obtain a traditional Chinese medicine composition for treating gastric cancer.

7. The extraction method according to claim 6, wherein The temperature of the concentration is 50°C - 80°C; the concentration ratio is 8 - 12.

8. The preparation method according to claim 6, characterized in that, The temperature of the freeze-drying is -50°C - -110°C; the pressure of the freeze-drying is 10 - 100 Pa.

9. Use of the traditional Chinese medicine composition according to claim 1 or 2 or the traditional Chinese medicine composition prepared by the preparation method according to any one of claims 3 - 8 in the preparation of a drug for treating and / or preventing gastric cancer.

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