CAR-T cell targeting NKG2DL and secreting IL-15 and preparation method thereof

By designing CAR-T cells that target NKG2DL and secrete IL-15, the problem of CAR-T cells maintaining short time in tumor treatment is solved, tumor killing activity and cell survival ability are enhanced, and more effective tumor treatment is achieved.

CN120399088APending Publication Date: 2025-08-01GUANGDONG PANGUARD CELL BIOLOGICAL TECH CO LTD
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Patent Information

Application Number
CN202510373306.X
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-03-27
Publication Date
2025-08-01

AI Technical Summary

Technical Problem

The existing CAR-T cell therapies have problems such as tumor recurrence, limited cell durability and tumor microenvironment inhibition in tumor treatment, especially the short maintenance time of CAR-T cells targeting NKG2DL, which limits the therapeutic effect.

Method used

A CAR-T cell targeting NKG2DL and secreting IL-15 is designed, including signal peptide region, NKG2D, IL-15R domain, hinge region, transmembrane region, intracellular signaling region, costimulatory region, insulin signaling peptide and IL-15 sequence. Through recombinant lentivirus transducing T cells, NKG2DL can be recognized and secreted IL-15 to enhance killing activity.

Benefits of technology

It improves the killing ability of CAR-T cells and the survival and proliferation ability of terminally depleted cells, enhances tumor killing activity, and prolongs the cell maintenance time.

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Abstract

The invention belongs to the field of biological medicine, and relates to a CAR-T cell targeting NKG2DL and secreting IL-15 and a preparation method thereof, the method comprises the step of infecting a T cell with a recombinant lentivirus, and the recombinant lentivirus contains a nucleic acid molecule of a chimeric antigen receptor encoding NKG2D and IL-15 or is prepared through an expression vector containing the nucleic acid molecule. The CAR-T cell expresses a chimeric antigen receptor targeting NKG2DL and can secrete a cell factor IL-15, the proliferation and effector functions of the CAR-T cell are promoted, and the killing activity on tumors is enhanced.
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