Targeting Claudin18.2 nano antibody and application thereof
By designing nano-antibody with specific amino acid sequences, the problem of low binding efficiency of targeting Claudin18.2 in the prior art was solved, and efficient tumor detection and treatment effects were achieved.
CN120484124AActive Publication Date: 2025-08-15HARBIN MEDICAL UNIVERSITY
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Patent Information
- Application Number
- CN202510633365.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-16
- Publication Date
- 2025-08-15
- Estimated Expiration
- 2045-05-16
AI Technical Summary
Technical Problem
The lack of nano-antibody that efficiently targets Claudin18.2 in the prior art makes it difficult to effectively bind and recognize the Claudin18.2 protein, limiting the accuracy and effectiveness of tumor treatment.
Method used
A nanoantibody targeting Claudin18.2, containing specific CDR and FR amino acid sequences, was designed and prepared, and was expressed and purified by in vitro engineering bacteria to achieve high affinity binding to Claudin18.2-positive cells.
Benefits of technology
The provided nano-antibodies have strong affinity with Claudin18.2-positive cells and are able to detect and treat Claudin18.2-expressed tumor tissues in vitro and in vitro, and have important commercial value.
✦ Generated by Eureka AI based on patent content.
Abstract
The invention discloses a Claudin18.2-targeting nano antibody and application thereof, and belongs to the technical field of molecular biology. Aiming at the unique advantages of a nano antibody in the prior art, the invention provides a nano antibody targeting Claudin18.2, the amino acid sequence of the targeting nano antibody is shown as SEQ ID NO.1, and the nano antibody has relatively strong affinity with Claudin18.2 positive cells 293T-Claudin18.2, and can be combined with Claudin18.2 protein expressed on the surfaces of the cells. The targeted Claudin18.2 nano antibody provided by the invention can be prepared through in-vitro engineering bacterium mass expression, can be applied to preparation of protein detection antibodies or therapeutic antibodies, can be used for in-vivo and in-vitro detection of tumor tissues expressed by Claudin18.2, and has important commercial value in clinical disease diagnosis and treatment.
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