Tomato seed disinfection method and application

Abstract

The invention relates to the technical field of seed treatment, in particular to a tomato seed disinfection method and application. The tomato seed disinfection method specifically comprises the following steps: S1, performing high-temperature treatment on virus-carrying seeds; step S2, soaking the virus-carrying seeds with a disinfectant; and S3, washing the seeds with sterile water for 3-5 times, 1-5 minutes each time, and drying at 30 DEG C to finish disinfection work. The method is reasonable in design, the disinfection method of physical high temperature and chemical disinfectant combined treatment is adopted, viruses, bacteria and fungi carried by the seeds can be thoroughly killed, and it is effectively ensured that the seeds are in a sterile state; the disinfection effect of the combined disinfection method is obviously better than that of independent treatment of high temperature and chemical disinfectants; meanwhile, the disinfection method does not influence the germination rate of the seeds and can shorten the average germination time of the seeds.

Description

Technical Field

[0001] The invention relates to the technical field of seed treatment, in particular to a method for disinfecting tomato seeds and application thereof. Background Art

[0002] Tomato virus diseases, also known as cancers of vegetables, are primarily transmitted through seed infection. Tomato seeds carry numerous viruses, including common ones such as Tomato chlorosis virus (ToCV), Tomato mottle mosaic virus (ToMMV), Southern tomato virus (STV), Tomato spotted wilt virus (TSWV), Cucumber mosaic virus (CMV), Tomato yellow leaf curl virus (TYLCV), Tobacco mosaic virus (TMV), Tobacco brown rugosefruit virus (ToBRFV), and Tomato mosaic virus (ToMV). After fruit from infected plants is produced as seed, the virus remains on or within the seeds, causing disease during the next round of growth. Symptoms include leaf mosaic, chlorotic and yellow veins, stunted plants, and uneven fruit coloring, severely impacting tomato yield and quality. At the same time, the convenience of seed transportation makes the spread of viral diseases very fast, posing a potential threat to areas where the virus has not yet occurred.

[0003] Tomatoes are an important vegetable in the Chinese diet, and their yield and quality directly impact food safety and residents' quality of life. Seed disinfection, a key measure for preventing seed-borne diseases, effectively kills viruses and other harmful microorganisms attached to or within seeds, thereby ensuring healthy tomato growth and a stable harvest, and improving farmers' economic benefits. Seed disinfection significantly reduces the incidence of later-stage diseases, reduces pesticide use, and meets consumer demand for high-quality vegetables. It also helps reduce agricultural non-point source pollution and maintain the balance of farmland ecosystems. Therefore, the application of seed disinfection technology has significant economic, social, and ecological benefits.

[0004] At present, the commonly used disinfection methods for tomato seeds mainly include soaking seeds in warm water and soaking seeds with chemicals. Although soaking seeds in warm water is simple to operate, its disinfection efficiency is low and it is difficult to completely kill a variety of pathogens. Although soaking seeds with chemicals can effectively kill microorganisms on the surface of seeds, its range of action is limited. Specific fungicides are usually only effective against one or several pathogens, and the mixed use of multiple agents can easily lead to chemical residues, affect seed vitality, and pose potential risks to human health and the ecological environment. In addition, the widespread use of fungicides has caused some pathogens to develop drug resistance, further weakening the effectiveness of chemical seed soaking. It is worth noting that research on seed disinfection technology for emerging seed-borne diseases in China (such as Tomato Brown Wrinkled Fruit Virus ToBRFV) is still blank, and there is an urgent need to develop safe and efficient disinfection technology to deal with the spread and spread of seed-borne diseases such as Tomato Brown Wrinkled Fruit Virus. Summary of the Invention

[0005] The technical problem to be solved by the present invention is to overcome the deficiencies in the prior art and provide a safe and efficient combined disinfection method and application for tomato seeds. The disinfection method of the present invention has the advantages of high disinfection rate and low drug residue.

[0006] The technical solution adopted by the present invention to solve its technical problem is:

[0007] A method for disinfecting tomato seeds specifically comprises the following steps:

[0008] Step S1, high temperature treatment of infected seeds; high temperature disinfection can destroy the viral protein structure, inactivate the virus on the surface and inside the seeds, and improve the disinfection efficiency;

[0009] Step S2: soaking the infected seeds in a disinfectant; the disinfectant soaking treatment can further kill the viruses that cannot be completely inactivated by high temperature, and the double treatment ensures thorough disinfection;

[0010] Step S3: Wash the seeds 3-5 times with sterile water for 1-5 minutes each time, and then dry them at 30°C to complete the disinfection. Washing the seeds can remove residual disinfectants, avoid negative effects on seed germination, and maintain seed activity.

[0011] High-temperature treatment synergizes with chemical disinfectants to deeply inactivate viruses on and within tomato seeds. Combined with sterile water washing and low-temperature drying, this method ensures thorough disinfection while minimizing damage to seed viability. This simple, highly controllable disinfection method is suitable for large-scale seed treatment and significantly reduces the risk of tomato virus disease transmission.

[0012] Furthermore, in step S1, the high temperature treatment temperature is 100-120°C for 1-4 hours. The high temperature of 100-120°C can effectively destroy viral protein structures and genetic material. The 1-4 hour treatment time balances inactivation efficiency with seed heat resistance, avoiding the decrease in embryo activity caused by prolonged high temperature exposure, and ensuring a germination rate of no less than 90% after disinfection.

[0013] Furthermore, in step S2, the disinfectant is one of sodium hypochlorite, trisodium phosphate, and potassium monopersulfate. Sodium hypochlorite releases active chlorine to destroy viral envelopes and nucleic acids, offering both broad-spectrum sterilization and low-cost advantages; trisodium phosphate chelates viral surface proteins and removes impurities from the seed surface, enhancing the permeability of subsequent disinfectants; and potassium monopersulfate, with its strong oxidizing properties, can penetrate the micropores of the seed epidermis and is significantly effective against stubborn viruses (such as tobacco mosaic virus).

[0014] Furthermore, in step S2, the effective chlorine content of the sodium hypochlorite is 0.5% to 2.5%. Limiting the effective chlorine content can balance the sterilization effect and seed safety.

[0015] Furthermore, in step S2, the mass concentration of trisodium phosphate is 5% to 15%. The alkaline environment destroys viral nucleic acids, and this concentration range ensures the disinfection effect without damaging the seeds.

[0016] Furthermore, in step S2, the mass concentration of potassium monopersulfate is 0.3% to 1%. Potassium monopersulfate has strong oxidizing properties and can effectively inactivate viruses at low concentrations, reducing chemical residues.

[0017] Furthermore, in step S2, the soaking time is 1 to 4 hours. The soaking time of 1 to 4 hours ensures that the disinfectant fully penetrates the microporous structure of the seeds, while preventing excessive water absorption from causing endosperm cell rupture, thereby maintaining seed vitality.

[0018] Furthermore, in step S2, during the disinfectant soaking process, an ultrasonic oscillator is used to perform 5 to 10 consecutive oscillations, each for 8 to 10 seconds. Ultrasonic oscillation enhances the efficiency of contact between the disinfectant and the virus, shortens the disinfection time, and improves the inactivation rate (especially for viruses in the crevices of the seeds). Specifically, pulsed ultrasound of 8 to 10 seconds per time peels off virus particles on the seed surface through the cavitation effect. 5 to 10 oscillations increase the penetration depth of the disinfectant by 40%, shorten the treatment time by more than 30%, and reduce the amount of chemical reagents used.

[0019] An application of the above-mentioned method for disinfecting tomato seeds, wherein the method is used in preventing and controlling tomato brown wrinkle fruit virus, tomato spotted wilt virus or tobacco mosaic virus.

[0020] The disinfection mechanism of the present invention is as follows:

[0021] Viruses consist of a protein shell and an internal nucleic acid. The disinfection mechanism of inorganic agents is to destroy the physiological structure of the virus, damaging its proteins and nucleic acids, thereby interfering with the transmission and reproduction of its genetic information. High-temperature disinfection, on the other hand, is achieved by denaturing the viral protein and nucleic acid biomacromolecules. The combined use of these two agents, acting on viral proteins and nucleic acids from different pathways, is like two elite troops attacking the same target from different directions, further strengthening the destruction of the ToBRFV virus structure and synergistically enhancing the inactivation effect of ToBRFV.

[0022] The beneficial effects of the present invention are as follows: the present invention has a reasonable design and adopts a disinfection method combining physical high temperature and chemical disinfectants, which can completely disinfect viruses, bacteria and fungi carried by seeds and effectively ensure that the seeds are in a sterile state; the disinfection effect of this combined disinfection method is significantly better than the disinfection effect of high temperature and chemical disinfectants alone; at the same time, the disinfection method does not affect the seed germination rate and can shorten the average germination time of seeds. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the specific embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0024] Figure 1 It is a bar chart comparing the disinfection rates of ToBRFV-infected tomato seeds under the single treatment and combined treatment of Example 8.

[0025] Figure 2 This is a bar graph comparing the disinfection rates of ToBRFV-infected tomato seeds under the single treatment and combined treatment of Example 9.

[0026] Figure 3 This is a bar chart comparing the disinfection rates of ToBRFV-infected tomato seeds under the single treatment and combined treatment of Example 10.

[0027] Figure 4 This is a diagram of tomato seed germination in the 15% trisodium phosphate disinfection treatment group and the control example in Example 11. DETAILED DESCRIPTION

[0028] It should be noted that the following detailed descriptions are illustrative and intended to provide further explanation of the present application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art to which the present application belongs.

[0029] It should be noted that the terms used herein are only for describing specific embodiments and are not intended to limit the exemplary embodiments according to the present application. As used herein, unless the context clearly indicates otherwise, the singular also includes the plural. In addition, it should be understood that when the terms "comprise" and / or "include" are used in this specification, they indicate the presence of features, steps, operations, devices, components and / or combinations thereof.

[0030] The following will clearly and completely describe the technical solutions of the present invention in conjunction with the embodiments. Obviously, the embodiments described are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0031] Example 1

[0032] The present embodiment provides a method for disinfecting tomato seeds, specifically: placing tomato seeds infected with Tomato Brown Rough Fruit Virus (ToBRFV), Tomato Spotted Wilt Virus (TSMV), and Tobacco Mosaic Virus (TMV) in ovens at 100°C, 110°C, and 120°C, respectively, and treating them at each temperature for 2 and 4 hours, respectively. The control treatment is: placing the poisonous tomato seeds in an oven at 25°C, and treating them for 2 and 4 hours, respectively. The virus content of the treated group and the control seeds was tested by RT-qPCR, and the disinfection rates under different temperature and time treatments were obtained through data statistics and analysis as shown in Table 1. The disinfection rate calculation formula is:

[0033] Disinfection rate = (virus content of control group - virus content of treatment group) / virus content of control group.

[0034] Table 1 Disinfection rate of tomato seeds under different temperature and time treatments

[0035]

[0036] As shown in Table 1, under high temperature treatment at 100℃~120℃, the disinfection rate of virus-carrying seeds increased with the increase of temperature. At 120℃, the disinfection rate of tomato seeds carrying different viruses reached 77.47%~83.35%.

[0037] Example 2

[0038] This embodiment provides a method for disinfecting tomato seeds, specifically: preparing 0.3%, 0.5% and 1% potassium monopersulfate solutions, soaking tomato seeds infected with ToBRFV, TSMV, and TMV in the above three reagents for 2 hours respectively. During the soaking period, an ultrasonic oscillator is used to perform 10 consecutive oscillations, each for 8 seconds; finally, the seeds are washed with sterile water 3 times for 5 minutes each time, and then dried at 30°C for 30 minutes to complete the treatment. The control example treatment is: soaking in sterile water at room temperature for 4 hours, and then drying at 30°C for 30 minutes. The virus content of the treated group and the control example seeds was tested by RT-qPCR, and the disinfection rates under different solution treatments were obtained through data statistics and analysis as shown in Table 2.

[0039] Table 2 Disinfection rate of poisonous tomato seeds treated with potassium monopersulfate at different concentrations

[0040]

[0041]

[0042] As shown in Table 2, under treatment with different concentrations of potassium monopersulfate, the disinfection rate of infected seeds increased with the increase of concentration. The disinfection rate of tomato seeds infected with ToBRFV was 62.56%~95.67%; the disinfection rate of tomato seeds infected with TSWV was 66.39%~85.40%; and the disinfection rate of tomato seeds infected with TMV was 65.50%~88.57%.

[0043] Example 3

[0044] This embodiment provides a method for disinfecting tomato seeds, specifically: preparing 0.5%, 1.5% and 2.5% sodium hypochlorite solutions, soaking tomato seeds infected with ToBRFV, TSMV and TMV in the above three reagents for 2 hours respectively, during which time an ultrasonic oscillator is used to perform 10 consecutive oscillations for 8 seconds each time; finally, the seeds are washed with sterile water 3 times for 5 minutes each time, and then dried at 30°C for 30 minutes to complete the treatment. The control treatment was as follows: soaking in sterile water at room temperature for 4 hours, and then drying at 30°C for 30 minutes. The virus content of the treated and control seeds was tested by RT-qPCR, and the disinfection rates under different solution treatments were obtained through data statistics and analysis as shown in Table 3.

[0045] Table 3 Disinfection rate of poisonous tomato seeds treated with sodium hypochlorite at different concentrations

[0046]

[0047] As shown in Table 3, under the treatment of different concentrations of sodium hypochlorite, the disinfection rate of infected seeds increased with the increase of concentration. The disinfection rate of tomato seeds infected with ToBRFV was 58.83%~87.64%; the disinfection rate of tomato seeds infected with TSWV was 59.17%~83.29%; and the disinfection rate of tomato seeds infected with TMV was 62.45%~85.37%.

[0048] Example 4

[0049] This embodiment provides a method for disinfecting tomato seeds, specifically: preparing 5%, 10%, and 15% trisodium phosphate solutions, soaking tomato seeds infected with ToBRFV, TSMV, and TMV in each of the three reagents for 2 hours. During the soaking period, an ultrasonic oscillator is used to continuously oscillate 10 times, each for 8 seconds; finally, the seeds are washed with sterile water 3 times for 5 minutes each time, and then dried at 30°C for 30 minutes to complete the treatment. The control example treatment was: soaking in sterile water at room temperature for 4 hours, and then drying at 30°C for 30 minutes. The virus content of the treated and control seeds was tested by RT-qPCR, and the disinfection rates under different solution treatments were obtained through data statistics and analysis as shown in Table 4.

[0050] Table 4 Disinfection rate of poisonous tomato seeds treated with different concentrations of trisodium phosphate

[0051]

[0052] As shown in Table 4, under the treatment of different concentrations of trisodium phosphate, the disinfection rate of infected seeds increased with the increase of concentration. The disinfection rate of tomato seeds infected with ToBRFV was 76.78%~99.82%; the disinfection rate of tomato seeds infected with TSWV was 70.67%~95.60%; and the disinfection rate of tomato seeds infected with TMV was 69.48%~89.33%.

[0053] Example 5

[0054] This example provides a method for disinfecting tomato seeds, specifically: placing tomato seeds infected with ToBRFV in an oven at 100°C for 2 hours; preparing different concentrations of treatment reagents: 0.3%, 0.5%, and 1% potassium monopersulfate solution; 5%, 1.5%, and 2.5% sodium hypochlorite solution; and 5%, 10%, and 15% trisodium phosphate solution; soaking the seeds in each of these solutions for 2 hours; during the soaking period, using an ultrasonic oscillator for 10 consecutive 8-second cycles; washing the seeds with sterile water three times for 5 minutes each, and drying them at 30°C for 30 minutes to complete the treatment. A control treatment was performed: soaking in sterile water at room temperature for 4 hours, followed by drying at 30°C for 30 minutes. Virus content in the treated and control seeds was measured by RT-qPCR. Data statistical analysis revealed the disinfection rates of the different solution treatments, as shown in Table 5.

[0055] Table 5 Disinfection rate of tomato seeds infected with ToBRFV under the combination of reagent + high temperature treatment

[0056] As shown in Table 5, under the combined treatment of reagent + high temperature 100°C, the disinfection rate of tomato seeds infected with virus (ToBRFV) reached more than 90.29%. The disinfection rate showed an upward trend with the increase of the concentration of the disinfectant, and could reach a maximum of 99.96%.

[0057] Example 6

[0058] This example provides a method for disinfecting tomato seeds, specifically comprising: placing tomato seeds infected with TSWV in an oven at 100°C for 2 hours; preparing treatment reagents of varying concentrations: 0.3%, 0.5%, and 1% potassium monopersulfate solutions; 5%, 1.5%, and 2.5% sodium hypochlorite solutions; and 5%, 19%, and 15% trisodium phosphate solutions; soaking the seeds in each of these solutions for 2 hours; and using an ultrasonic oscillator for 10 consecutive 8-second cycles during the soaking period. The seeds were then rinsed with sterile water three times for 5 minutes each, and then dried at 30°C for 30 minutes to complete the treatment. A control treatment was performed: soaking in sterile water at room temperature for 4 hours, followed by drying at 30°C for 30 minutes. The virus content in the treated and control seeds was determined by RT-qPCR. Data statistical analysis revealed the disinfection rates of the different solutions, as shown in Table 6.

[0059] Table 6 Disinfection rate of TSWV-infected tomato seeds under combined treatment of disinfectant + high temperature 100℃ As shown in Table 6, under the combined treatment of reagent + 100°C, the disinfection rate of tomato seeds infected with TSWV reached more than 85.33%. The disinfection rate showed an upward trend with the increase of the concentration of the disinfectant, and the highest rate could reach 99.92%.

[0060] Example 7

[0061] This example provides a method for disinfecting tomato seeds, specifically comprising: placing tomato seeds infected with TMV in an oven at 100°C for 2 hours; preparing treatment reagents of varying concentrations: 0.3%, 0.5%, and 1% potassium monopersulfate solutions; 5%, 1.5%, and 2.5% sodium hypochlorite solutions; and 5%, 19%, and 15% trisodium phosphate solutions; soaking the seeds in each of these solutions for 2 hours; and using an ultrasonic oscillator for 10 consecutive 8-second cycles during the soaking period. The seeds were then rinsed with sterile water three times for 5 minutes each, and then dried at 30°C for 30 minutes to complete the treatment. A control treatment was performed: soaking in sterile water at room temperature for 4 hours, followed by drying at 30°C for 30 minutes. The virus content in the treated and control seeds was measured by RT-qPCR. Data statistical analysis revealed the disinfection rates of the different solutions, as shown in Table 7.

[0062] Table 7 Disinfection rate of TMV-infected tomato seeds under the combined treatment of disinfectant + high temperature 100℃

[0063]

[0064] As shown in Table 7, under the combined treatment of reagent + 100°C, the disinfection rate of tomato seeds infected with TMV reached more than 86.67%. The disinfection rate showed an upward trend with the increase of the concentration of the disinfectant, and the highest rate could reach 99.89%.

[0065] Example 8

[0066] This example provides a comparison of the disinfection rates of different disinfection measures for poisonous tomato seeds. The three types of data are compared: the disinfection rate of ToBRFV-infected seeds under high temperature treatment at 100°C in Example 1, the disinfection rate of ToBRFV-infected seeds under treatment with different concentrations of disinfectants in Examples 2 to 4, and the disinfection rate of ToBRFV-infected seeds under combined treatment with different concentrations of disinfectants + 100°C in Example 5, and the difference in disinfection rate between combined treatment and individual treatment is analyzed. The test results are shown in Table 8, and plotted. Figure 1 .

[0067] Table 8 Disinfection rate of ToBRFV-infected tomato seeds under different treatments

[0068]

[0069]

[0070] Note: Different letters in the same row indicate significant differences between the data by Duncan's multiple comparison (P < 0.05).

[0071] From Table 8 and Figure 1It can be seen that the disinfection rate of ToBRFV-infected seeds treated with 1% potassium monopersulfate was 95.67%, which was lower than the disinfection rate of 99.83% treated with 1% potassium monopersulfate + 100℃, and there was no significant difference between the two. The disinfection rate of ToBRFV-infected seeds treated with 15% trisodium phosphate was 99.82%, which was equivalent to the disinfection rate of 99.96% treated with 15% trisodium phosphate + 100℃, and there was no significant difference between the two. In addition, the disinfection rate of ToBRFV-infected seeds under the combined treatment of reagent + 100℃ was significantly higher than the disinfection rate under the treatment of reagent / 100℃ alone.

[0072] Example 9

[0073] This example provides a comparison of the disinfection rates of different disinfection measures for tomato seeds infected with poison. The disinfection rates of TSWV-infected seeds treated with high temperature at 100°C in Example 1, the disinfection rates of TSWV-infected seeds treated with different concentrations of disinfectants in Examples 2 to 4, and the disinfection rates of TSWV-infected seeds treated with different concentrations of disinfectants + 100°C in Example 6 were compared to analyze the difference in disinfection rates between the combined treatment and the individual treatments. The test results are shown in Table 9 and plotted. Figure 2 .

[0074] Table 9 Disinfection rate of TSWV-infected tomato seeds under different treatments

[0075]

[0076] Note: Different letters in the same row indicate significant differences between the data by Duncan's multiple comparison (P < 0.05).

[0077] From Table 9 and Figure 2 The results show that the disinfection rate of 15% trisodium phosphate treatment against TSWV-infected seeds was 95.60%, comparable to the 98.43% disinfection rate of 15% trisodium phosphate + 100°C treatment, with no significant difference between the two. Furthermore, the disinfection rate of TSWV-infected seeds under the combined treatment of the reagent + 100°C was significantly higher than that under either the reagent or 100°C treatment alone.

[0078] Example 10

[0079] This example provides a comparison of the disinfection rates of different disinfection measures for poisonous tomato seeds. The three types of data are compared: the disinfection rate of TMV-infected seeds under high temperature treatment at 100°C in Example 1, the disinfection rate of TMV-infected seeds under treatment with different concentrations of disinfectants in Examples 2 to 4, and the disinfection rate of TMV-infected seeds under combined treatment with different concentrations of disinfectants + 100°C in Example 7. The difference in disinfection rate between the combined treatment and the individual treatment is analyzed. The test results are shown in Table 10 and plotted. Figure 3 .

[0080] Table 10 Disinfection rate of TMV-contaminated tomato seeds under different treatments

[0081]

[0082] Note: Different letters in the same row indicate significant differences between the data by Duncan's multiple comparison (P < 0.05).

[0083] From Table 10 and Figure 3 It can be seen that the disinfection rate of TMV-infected seeds under the combined treatment of reagent + 100℃ was significantly higher than the disinfection rate under the single treatment of reagent / 100℃.

[0084] Example 11

[0085] The seeds disinfected in Example 5 were placed in a light incubator and cultured at a temperature of 25-28°C, a humidity of 60-80%, and a day / night ratio of 14:10 for 3-10 days. The number of seeds that germinated, the germination time, and the root length of the treated and control groups were measured, and the seed germination rate, average germination time, and average root length were calculated using the following formulas. The results are shown in Table 11.

[0086] Seed germination rate (%) = number of germinated seeds / total number of treated seeds × 100

[0087] Average seed germination time = ∑ germination time of all seeds / number of seeds

[0088] Average root length of seeds = ∑ root length of all seeds / number of seeds

[0089] The treatment in Example 5 did not significantly affect the germination rate and root length of the tomato seeds in the treatment group; the average germination time of the seeds in the treatment group was shortened, and the average germination time of the seeds in the 2.5% sodium hypochlorite treatment group was significantly shorter than that in the control group.

[0090] Table 11 Effects of combined disinfection treatments on tomato seed germination and growth

[0091]

[0092] Note: The data shown in the table are the mean ± standard error of three replicates. The same letters in the same column indicate no significant difference between the data by Duncan's multiple comparison (P < 0.05).

[0093] Figure 4 This is a graph showing the germination of tomato seeds in the 15% trisodium phosphate disinfection treatment group and the control example in Example 11. As can be seen from the graph, the germination and growth effects of the tomato seeds in the 15% trisodium phosphate disinfection treatment group were better.

[0094] In summary, the seed disinfection technology of the present invention is novel and has the following advantages:

[0095] (1) The tomato seed disinfection technology of the present invention has a significant disinfection effect and can be used to kill plant viruses such as Tomato Brown Rough Fruit Virus (ToBRFV), Tomato Spotted Wilt Virus (TSMV), and Tobacco Mosaic Virus (TMV) carried by seeds of tomatoes and tobacco;

[0096] (2) The disinfection rate of the chemical reagent + physical high temperature combined disinfection measure of the present invention is generally better than the disinfection rate under the reagent / high temperature treatment alone.

[0097] (3) The tomato seed disinfection technology of the present invention has a high disinfection rate and has no adverse effects on seed germination and growth. Some treatment methods (the 2.5% sodium hypochlorite treatment group in Example 11) can also significantly shorten the germination time of tomato seeds;

[0098] (4) The combined tomato seed disinfection technology of the present invention has good environmental compatibility and no risk of pesticide residues;

[0099] (5) The disinfection technology of the present invention is simple to use, has a significant disinfection effect, and is suitable for promotion and use.

[0100] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the above embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for disinfecting tomato seeds, characterized in that: The specific steps include: Step S1, high temperature treatment of poisonous seeds; Step S2, soaking the poisoned seeds in a disinfectant; Step S3: Wash the seeds 3 to 5 times with sterile water, each time for 1 to 5 minutes, and dry them at 30°C to complete the disinfection.

2. The method for disinfecting tomato seeds according to claim 1, wherein: In the step S1, the high temperature treatment temperature is 100-120° C., and the treatment time is 1-4 hours.

3. The method for disinfecting tomato seeds according to claim 1, wherein: In step S2, the disinfectant is one of sodium hypochlorite, trisodium phosphate, and potassium monopersulfate.

4. The method for disinfecting tomato seeds according to claim 3, wherein: In step S2, the available chlorine content of sodium hypochlorite is 0.5% to 2.5%.

5. The method for disinfecting tomato seeds according to claim 3, characterized in that: In step S2, the mass concentration of trisodium phosphate is 5% to 15%.

6. The method for disinfecting tomato seeds according to claim 3, characterized in that: In step S2, the mass concentration of potassium monopersulfate is 0.3% to 1%.

7. The method for disinfecting tomato seeds according to claim 1, characterized in that: In step S2, the soaking time is 1 to 4 hours.

8. The method for disinfecting tomato seeds according to claim 1, wherein: In step S2, during the disinfectant soaking process, an ultrasonic oscillator is used to perform 5 to 10 consecutive oscillations, each oscillation lasting 8 to 10 seconds.

9. Use of the method for disinfecting tomato seeds according to any one of claims 1 to 8, characterized in that: The disinfection method is used in preventing and controlling tomato brown wrinkle virus, tomato spotted wilt virus or tobacco mosaic virus.

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