Acaricidal composition and application thereof

Abstract

The invention belongs to the technical field of pesticide mite killing, and discloses a mite killing composition and application thereof.The mite killing composition comprises an active component A and an active component B. The active component A is a compound shown in the formula I: # imgabs0, the active component B is selected from clofentezine or hexythiazox, and the mass ratio of the active component A to the active component B is 1: 50-50: 1. The acaricidal composition disclosed by the invention can be used for preventing and controlling spreading and damage of common pest mites in agriculture or forestry, has an obvious synergistic effect and is safe to natural enemy beneficial insects.

Description

[0001] This invention application is a divisional application with application number 202311021235.4, application date August 15, 2023, and invention name “A mite-killing composition and its application”. Technical Field

[0002] The invention belongs to the technical field of pesticide acaricide and discloses an acaricide composition and application thereof. Background Art

[0003] Mites are arachnid pests and are one of the important pests of many crops in my country. They are small in size, reproduce quickly, mutate quickly, and are easy to produce.

[0004] Chemical control is very difficult.

[0005] In addition to chemical control, biological control can effectively control the damage of pest mites while reducing the dosage of pesticides.

[0006] The relationship between biological control and chemical control, reducing the impact of pesticides on the environment, natural enemies and pest resistance, and seeking chemical pesticides that are highly effective against target pests and have less impact on natural enemies are particularly important.

[0007] To this end, the applicant of the present invention selected the compound of formula I and any one of hexythiazox or clofentezine for reasonable compound use, determined the optimal ratio for synergistic effect on target pest mites and safety to natural enemies and beneficial insects, reduced the dosage of the agent, and protected the natural enemies of the pest mites to the greatest extent.

[0008] It provides a basis for the rational use of acaricides, enables safe use of pesticides, and reduces the impact of pesticides on the environment and natural enemies. Summary of the Invention

[0009] Based on the problems in the prior art, the present invention provides a mite-killing composition, which has a good synergistic effect on common plant pests and has little toxicity to natural enemies and beneficial insects, thus reducing the dosage of the agent, and protecting natural enemies and beneficial insects to the greatest extent while preventing and controlling the pests.

[0010] The acaricide composition can kill enemy organisms, and the preparation prepared by the acaricide composition has excellent fast-acting effect and long lasting effect, thereby reducing environmental pollution.

[0011] In order to achieve the above object, the present invention adopts the following technical solution: a mite-killing composition, the mite-killing composition comprises active ingredient A and active ingredient B, the active ingredient A is a compound represented by formula I: The active ingredient B is selected from any one of hexythiazox or clofentezine;

[0012] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:50 to 50:1;

[0013] Furthermore, the active ingredient B is hexythiazox, and the mass ratio of the active ingredient A to the active ingredient B is 1:45 to 45:1;

[0014] The active ingredient B is clofentezine, and the mass ratio of the active ingredient A to the active ingredient B is 1:42 to 36:1.

[0015] Furthermore, the active ingredient B is hexythiazox, and the mass ratio of the active ingredient A to the active ingredient B is 1:22 to 35:1;

[0016] The active ingredient B is clofentezine, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 30:1;

[0017] Furthermore, the acaricidal composition further comprises an auxiliary component, which is a carrier or an adjuvant;

[0018] Furthermore, the wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium lauryl sulfate, sodium dioctyl sulfosuccinate, α-olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, soapberry powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0019] The dispersant is selected from one or more of lignin sulfonates, alkylnaphthalene sulfonate formaldehyde condensates, naphthalene sulfonates, tristyrylphenol ethoxylate phosphates, fatty alcohol ethoxylates, alkylphenol polyoxyethylene ethers, alkylphenol polyoxyethylene ether formaldehyde condensate sulfates, fatty amine polyoxyethylene ethers, glycerol fatty acid ester polyoxyethylene ethers, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0020] The thickener is selected from one or more of xanthan gum, polyvinyl alcohol, organic bentonite, magnesium aluminum silicate, and carboxymethyl cellulose; and / or

[0021] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0022] The emulsifier is selected from one or more of fatty alcohol polyoxyethylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, phenylethylphenol polyoxyethylene polyoxypropylene ether, alkylphenol polyoxyethylene ether, fatty amine polyoxyethylene ether, alkylbenzene sulfonate, styrylphenol polyoxyethylene ether, fatty acid polyoxyethylene ester; and / or

[0023] The defoaming agent is selected from silicone oil, C 10 ~C 20 Saturated fatty acid compounds, C8~C 10 One or more of fatty alcohol compounds or silicone compounds; and / or

[0024] The preservative is selected from one or more of sorbic acid, sodium sorbate, potassium sorbate, benzoic acid, sodium benzoate, sodium p-hydroxybenzoate, and methyl p-hydroxybenzoate; and / or

[0025] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, and epoxidized vegetable oil; and / or

[0026] The synergist is selected from synergist phosphine and piperonyl butoxide; and / or

[0027] The carrier is selected from one or more of kaolin, bentonite, attapulgite, light calcium carbonate, diatomaceous earth, and white carbon black; and / or

[0028] The solvent is selected from one or more of benzene, toluene, xylene, methanol, ethanol, isopropanol, n-butanol, diesel, N,N-dimethylformamide, cyclohexanone, ethyl acetate, N-methylpyrrolidone, propanol, butanol, ethylene glycol, diethylene glycol, ethylene glycol methyl ether, butyl ether, solvent oil, vegetable oil, vegetable oil derivatives and deionized water; and / or

[0029] All of the above auxiliary ingredients are commercially available.

[0030] Furthermore, the total weight of the active ingredient A and the active ingredient B accounts for 0.5% to 80% of the total weight of the acaricidal composition;

[0031] Furthermore, the acaricidal composition can be prepared into a liquid preparation or a solid preparation, wherein the liquid preparation is selected from a soluble solution, a soluble gel, an oil, a film-spreading oil, an emulsifiable concentrate, a latex, a dispersible liquid, an ointment, an aqueous emulsion, an oil emulsion, a microemulsion, a lipid suspension, a microcapsule suspension, an oil suspension, a dispersible oil suspension, a suspoemulsion, a microcapsule suspension-suspension, a microcapsule suspension-water emulsion or a microcapsule suspension-suspoemulsion; and the solid preparation is selected from a powder, a granule, a pellet, a tablet, a strip, a wettable powder, an oil-dispersible powder, an emulsion powder, a water-dispersible granule, an emulsion granule, a water-dispersible tablet, a soluble powder, a soluble tablet or a soluble granule.

[0032] Furthermore, the formulation is in the form of a suspension, emulsifiable concentrate, microemulsion, aqueous emulsion, water-dispersible granules or wettable powder.

[0033] The present invention also discloses the use of the acaricidal composition for preventing and controlling plant pests.

[0034] Furthermore, the plants are agricultural crops or forestry crops;

[0035] Furthermore, the plant pest mites are Tetranychus, Tenebrionidae, Mylopodidae and / or Eupodidae pest mites;

[0036] Furthermore, the pest mites of the Tetranychidae family are two-spotted spider mites, cinnabarinus spider mites, truncate spider mites, apple panonychus, citrus panonychus and / or wheat rock mite; the pest mites of the Gall Mite family are citrus rust louse, wheat gall louse and / or wolfberry gall mite; the pest mite of the True Tetranychidae family is wheat red spider.

[0037] The beneficial effects of the present invention are as follows: the acaricidal composition of the present invention rationally compounds acaricides with different action mechanisms, exhibits a good synergistic effect on common harmful mites, is safe to natural enemies, reduces the dosage of the agents, has good quick-acting properties, and has a long lasting effect. DETAILED DESCRIPTION

[0038] In order to make the technical solutions, objectives and advantages of the present invention more clearly understood, the present invention is described with reference to the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0039] Preparation example:

[0040] Preparation Example 1: 14% Formula I compound·hexythiazox aqueous emulsion (1:6)

[0041] Formula: 2% compound of formula I, 12% hexythiazolin, 1% Guerbet alcohol polyoxyethylene ether, 6% alkyl aryl polyoxyethylene ether polyoxypropylene ether, 8% cyclohexanone, 0.2% xanthan gum, 5% ethylene glycol, 0.1% sodium benzoate, 0.1% silicone oil, and deionized water to make up the balance;

[0042] Preparation method: According to the formulation ratio of the preparation example, the active ingredient is dissolved in the solvent and an emulsifier is added to dissolve it into a uniform oil phase. Deionized water and antifreeze are mixed together to form a uniform water phase; under high-speed shear, the water phase is added to the oil phase to form a well-dispersed water emulsion product.

[0043] Preparation Example 2: 5% Formula I compound·Hexythiazox microemulsion (4:1)

[0044] Formula: 4% compound of formula I, 1% hexythiazox, 1% alkylphenol polyoxyethylene ether phosphate, 10% styrylphenol polyoxyethylene ether, 2% sodium fatty alcohol polyoxyethylene ether sulfate, 2% calcium dodecylbenzenesulfonate, 5% alkyl glycoside, 2% xylene, 7% cyclohexanone, 3% ethylene glycol, 0.1% kason, 0.5% glycerol, 0.1% xanthan gum, and deionized water to make up the balance;

[0045] Preparation method: According to the formulation ratio of the preparation example, the active ingredient, solvent, emulsifier, etc. are mixed to prepare the oil phase, the antifreeze agent and water are mixed to prepare the aqueous phase, the oil phase is added to the aqueous phase under stirring and stirred evenly, and shearing is continued for 10 minutes. Then, silicone oil, defoaming agent, xanthan gum, etc. are added and stirred evenly to obtain small droplets of oil phase particles with a diameter of 0.01 to 0.1 microns, thereby preparing the microemulsion described in the present invention.

[0046] Preparation Example 3: 9% Formula I compound·hexythiazox emulsifiable concentrate (8:1)

[0047] Formula: 8% compound of formula I, 1% hexythiazox, 10% DMF, 8% alkylphenol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 15% propylene carbonate, and xylene makes up the balance;

[0048] Preparation method: add the measured active ingredients, solvent and cosolvent into a mixing kettle and stir to dissolve them, then add emulsifier, make up the balance with the remaining solvent, stir evenly in a stirring kettle, and filter to obtain the desired emulsifiable concentrate of the present invention.

[0049] Preparation Example 4: 22% Formula I compound·hexythiazox wettable powder (10:1)

[0050] Formula: 20% compound of formula I, 2% hexythiazox, 3% sodium salt of polycarboxylate, 8% naphthalenesulfonate formaldehyde condensate, 2% pulverized powder BX, 5% white carbon black, and kaolin to make up the balance;

[0051] Preparation method: The active ingredients, dispersant, wetting agent and filler are mixed according to the formula ratio, uniformly stirred in a stirring kettle, and pulverized and mixed uniformly for multiple times in a jet mill to prepare the wettable powder of the composition of the present invention.

[0052] Preparation Example 5: 18% Formula I compound·hexythiazox suspension (5:1)

[0053] Formula: 15% compound of formula I, 3% hexythiazolin, 2% isotridecyl polyoxyethylene ether, 4% styrenated phenol polyoxyethylene ether phosphate, 1% sodium lignin sulfonate, 0.25% xanthan gum, 3% ethylene glycol, 0.5% kason, 0.5% silicone oil, and deionized water to make up the balance;

[0054] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added and mixed evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0055] Preparation Example 6: 18% Formula I compound·clofentezine suspension (1:8)

[0056] Formula: 2% compound of formula I, 16% clofentazine, 1% alkylaryl polyoxyethylene ether polyoxypropylene ether, 1% sodium lignin sulfonate, 3% styrenated phenol polyoxyethylene ether sulfate, 2% fatty alcohol polyoxyethylene ether, 0.5% magnesium aluminum silicate, 0.2% xanthan gum, 5% ethylene glycol, 0.2% potassium benzoate, 0.5% silicone oil, and deionized water to make up the balance;

[0057] Preparation method: Same as Preparation Example 5.

[0058] Preparation Example 7: 18% Formula I compound·clofentezine wettable powder (8:1)

[0059] Formula: 16% compound of formula I, 2% clofentazine, 4% sodium polycarboxylate, 8% sodium lignin sulfonate, 2% pulverized powder BX, 5% white carbon black, and kaolin to make up the balance;

[0060] Preparation method: same as Preparation Example 4.

[0061] Preparation Example 8: 33% Formula I compound·clofentezine water dispersible granules (10:1)

[0062] Formula: 30% compound of formula I, 3% clofentezine, 1% succinate sulfonate, 10% naphthalenesulfonate formaldehyde condensate, 5% polycarboxylate sodium salt, 1% sodium lauryl sulfate, 5% white carbon black, 30% starch, and kaolin makes up the balance.

[0063] Preparation method: According to the formula ratio of the embodiment, the active ingredient is added to the carrier, and a surfactant and other functional additives are added thereto, mixed, and after air flow grinding, 10-25% water is added, and then kneading, granulation, drying, and sieving are carried out to obtain a water-dispersible granule product; or the pulverized powder is sprayed with water in a boiling granulator, granulated, dried, and then sieved to obtain the product.

[0064] Indoor activity test

[0065] Example 1: Indoor biological activity test of the compound of formula I in combination with clofentezine or hexythiazox against spider mites

[0066] The indoor test was conducted with reference to NY / T 1154.12-2008 “Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides Part 12: Spider Mite Slide Dipping Method”.

[0067] Biological test materials: nymphs of Tetranychus urticae.

[0068] Material Preparation: Select nymphs of mites raised indoors and in consistent physiological condition. Cut double-sided tape into 2 cm lengths and attach to one end of a glass slide. Select healthy mites and attach their backs to the tape, 30 per slide. Place mites in a damp sponge-lined insect box, close the lid, and store at (25 ± 1)°C. After 2 hours, examine under a microscope, remove dead or injured individuals, and replenish the remaining mites per slide.

[0069] Preparation of the drug: The technical of the compound of formula I, clofentezine and hexathiacloprid were dissolved in acetone to prepare a mother liquor, and then 5 series of mass concentrations were prepared in equal proportions using a 0.1% Tween-80 aqueous solution.

[0070] Chemical treatment: Immerse the glass slide in the chemical and shake it gently for 5 seconds, then take it out and absorb the excess liquid with absorbent paper. Place it in a white porcelain dish with a wet sponge and cover it with a light-transmitting plastic film.

[0071] Each treatment was repeated 4 times, and a treatment without drug (containing all organic solvents and emulsifiers) was set as a blank control.

[0072] Rearing and observation: Place the container containing the treated test insects under suitable conditions for rearing and observation.

[0073] Inspection: 48 hours after treatment, check the mortality of test insects and record the total number of insects and the number of dead insects respectively.

[0074] Data statistics and analysis:

[0075] Based on the survey data, the adjusted mortality rate of each treatment was calculated according to the following formula, and the calculation results were rounded to two decimal places.

[0076]

[0077] Where:

[0078] P——mortality rate, in percentage (%);

[0079] K——indicates the number of dead insects, the unit is head;

[0080] N——represents the total number of insects processed, in heads.

[0081]

[0082] Where:

[0083] P1——adjusted mortality rate, in percentage (%);

[0084] P t ——Treatment mortality rate, expressed in percentage (%);

[0085] P0 - blank control mortality rate, in percentage (%).

[0086] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the correction mortality formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0087] Analyze using the DPS statistical analysis system to determine the LC 50 The activity of the test agent on the biological test material is evaluated by the value.

[0088] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0089]

[0090] Where:

[0091] ATI - measured toxicity index of mixture;

[0092] S——LC of standard acaricide 50 , the unit is milligrams per liter (mg / L);

[0093] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0094] TTI=TI A ×P A +TI B ×P B

[0095] Where:

[0096] TTI – Theoretical Toxicity Index of Mixtures;

[0097] TI A ——Agent toxicity index;

[0098] P A ——The percentage of agent A in the mixture, in percentage (%);

[0099] TI B ——Toxicity index of agent B;

[0100] P B ——The percentage of agent B in the mixture, in percentage (%).

[0101]

[0102] Where:

[0103] CTC – Co-toxicity coefficient;

[0104] ATI - measured toxicity index of mixture;

[0105] TTI - Theoretical Toxicity Index of Mixture.

[0106] A co-toxicity coefficient (CTC) of ≥120 indicates a synergistic effect; a CTC of ≤80 indicates an antagonistic effect; and a CTC of 80 < CTC <120 indicates an additive effect. The results of the laboratory test are shown in the table below:

[0107] Table 1 Indoor biological activity test of compound of formula I combined with hexythiazox against nymphs of Tetranychus urticae

[0108] Test drug <![CDATA[LC 50 (mg / L)]]> (ATI) (TTI) Co-toxicity coefficient (CTC) Compound (A) of formula I 2.5833 100.000 / / <![CDATA[Hexythiazox (B1)]]> 11.4095 22.642 / / <![CDATA[A:B1=1:45]]> 10.0802 25.627 24.323 105.362 <![CDATA[A:B1=1:22]]> 8.0102 32.250 26.005 124.015 <![CDATA[A:B1=1:15]]> 6.9503 37.168 27.477 135.272 <![CDATA[A:B1=1:8]]> 5.1082 50.572 31.237 161.896 <![CDATA[A:B1=4:1]]> 1.4992 172.312 84.528 203.851 <![CDATA[A:B1=8:1]]> 1.4924 173.097 91.405 189.374 <![CDATA[A:B1=12:1]]> 1.6093 160.523 94.049 170.680 <![CDATA[A:B1=20:1]]> 1.8227 141.729 96.316 147.150 <![CDATA[A:B1=25:1]]> 1.9812 130.391 97.025 134.389 <![CDATA[A:B1=35:1]]> 2.1049 122.728 97.851 125.423

[0109] Table 2 Indoor biological activity test of compound of formula I combined with clofentezine on nymphs of Tetranychus urticae

[0110] Test drug <![CDATA[LC 50 (mg / L)]]> (ATI) (TTI) Co-toxicity coefficient (CTC) Compound (A) of formula I 2.5833 100.000 / / <![CDATA[Clofentezine (B2)]]> 6.0274 42.859 / / <![CDATA[A:B2=1:42]]> 4.6004 56.154 44.188 127.079 <![CDATA[A:B2=1:21]]> 3.9406 65.556 45.457 144.217 <![CDATA[A:B2=1:11]]> 3.4753 74.333 47.621 156.093 <![CDATA[A:B2=1:5]]> 2.4377 105.973 52.383 202.305 <![CDATA[A:B2=4:3]]> 1.5097 171.113 75.511 226.607 <![CDATA[A:B2=7:1]]> 1.5554 166.086 92.857 178.861 <![CDATA[A:B2=10:1]]> 1.6932 152.569 94.805 160.929 <![CDATA[A:B2=15:1]]> 1.8856 137.001 96.429 142.075 <![CDATA[A:B2=22:1]]> 2.0573 125.567 97.516 128.767 <![CDATA[A:B2=36:1]]> 2.1081 122.542 98.456 124.464

[0111] As shown in Tables 1 and 2, the compound of Formula I, clofentezine, and hexathiazox each exhibited high toxicity against two-spotted spider mites, with the compound of Formula I showing the greatest control efficacy against them. The compound of Formula I and hexathiazox exhibited no antagonistic effect against two-spotted spider mites in a ratio range of 1:45 to 35:1. In the range of 1:22 to 25:1, the co-toxicity coefficient was greater than 120, indicating a synergistic effect. The compound of Formula I and clofentezine exhibited a co-toxicity coefficient greater than 120 in a ratio range of 1:42 to 36:1, indicating a synergistic effect.

[0112] Example 2: Safety test of the compound of formula I combined with clofentezine or hexythiazox against the natural enemy Coccinella polymorpha

[0113] Test target: 3rd instar larvae of Coccinella multifera.

[0114] Test agent: The original drug was prepared into a stock solution with an organic solvent and then dissolved in a 0.1% Tween-80 aqueous solution. The agent was prepared according to the optimal ratio of the compound of Formula I in Example 1 and clofentezine or hexythiazox. A treatment without the agent (containing all organic solvents and emulsifiers) was set as a blank control.

[0115] Material Preparation: Select clean, uniformly grown cotton leaves that have not been treated with pesticides. Cut them into 6-cm diameter leaf discs and spread them flatly in a plastic cup containing agar. Inoculate the leaf discs with 200 indoor-reared spider mites. Inoculate each cup with one 4-hour-starved Coccinella multicolor larva.

[0116] Chemical treatment: Place the plastic cup in a potter spray tower for spray treatment. After the liquid settles for 1 minute, take it out and seal the top of the cup with a gauze with a pore size of 0.15 mm to prevent the test insects from escaping.

[0117] Rearing and observation: The treated test insects were placed in a temperature of (25±1)℃, relative humidity of (70±5)%, and a photoperiod of L:D=(16:8)h for rearing and observation. After 48h, the mortality of the multicolored ladybirds was checked and the number of deaths was recorded. Insects that did not move when lightly touched with a brush were considered dead.

[0118] Each concentration of the drug and the control were repeated four times, and 30 third-instar larvae of Heteroptera multicolor were tested in each repeat.

[0119] Data statistics and analysis:

[0120] Based on the survey data, the adjusted mortality rate of each treatment was calculated according to the following formula, and the calculation results were rounded to two decimal places.

[0121]

[0122] Where:

[0123] P——mortality rate, in percentage (%);

[0124] K——indicates the number of dead insects, the unit is head;

[0125] N——represents the total number of insects processed, in heads.

[0126]

[0127] Where:

[0128] P1——adjusted mortality rate, in percentage (%);

[0129] P t ——Treatment mortality rate, expressed in percentage (%);

[0130] P0 - blank control mortality rate, in percentage (%).

[0131] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the correction mortality formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0132] Use DPS statistical analysis system to analyze and calculate the toxicity regression equation and LC 50 The activity of the test agent on the biological test material is evaluated by the value.

[0133] Safety factors were used to evaluate the safety of various pesticides against Coccinella heteroptera:

[0134]

[0135] When the toxicity selection index STR < 1, it indicates that the agent has a negative selectivity for natural enemies and pests (mites); when STR = 1, it means that the agent has no obvious selectivity for natural enemies and pests (mites); when 1 < STR ≤ 10, it indicates that the agent has a positive selectivity for natural enemies and pests (mites); when 10 < STR ≤ 100, it means that the agent has a moderate positive selectivity for natural enemies and pests; when 100 < STR ≤ 1000, it indicates that the agent has a high positive selectivity for natural enemies and pests (mites); when STR > 1000, it means that the agent has a strong positive selectivity for natural enemies and pests (mites).

[0136] Table 3 Results of the safety test of the compound of Formula I in combination with clofentezine or hexythiazox against the natural enemy Hippodamia variegata

[0137]

[0138] As can be seen from Table 3, the toxicity of the compound of Formula I in combination with clofentezine or hexythiazox against Hippodamia variegata is significantly lower than its toxicity against pest mites, and it is relatively safe for natural enemy insects.

[0139] Field efficacy test

[0140] Example 3: Field efficacy test of the acaricidal composition against Tetranychus cinnabarinus on cotton

[0141] In the cotton field of Yuanzhuang Village, Thirty Li Pu Town, Gaotang County, Liaocheng City, Shandong Province, the soil fertility is medium, and the cultivation conditions of all test plots are uniform and consistent with the local agricultural cultivation measures.

[0142] Test target: Tetranychus cinnabarinus.

[0143] Test method: The medicine was applied once at the initial stage of the occurrence of Tetranychus cinnabarinus. During the application, a knapsack sprayer was used for quantitative spraying, and no other pesticides for controlling pests and diseases were used during the test period.

[0144] Test investigation: Investigations were carried out 3 days, 7 days, and 14 days after the application. A total of 3 investigations were carried out. 20 leaves were fixed in each plot during the investigation, the number of active mites was counted, and the mite population reduction rate and control effect were calculated.

[0145] Safety investigation: At the same time of each test investigation, observe whether each treatment agent has an abnormal effect on cotton growth and whether there is an adverse effect on non-target organisms.

[0146] Test data statistics:

[0147]

[0148] The test results are shown in the following table:

[0149] Table 4 Results of the field efficacy test of the acaricidal composition against Tetranychus cinnabarinus on cotton

[0150]

[0151] Field efficacy trials showed that the acaricidal compositions of the present invention effectively control the damage caused by red spider mites on cotton plants. Each formulation exhibited rapid effectiveness and a long-lasting effect. Throughout the trials, no abnormal effects of the treatment agents on cotton growth or adverse effects on non-target organisms were observed.

[0152] Example 4: Field efficacy test of acaricidal composition against apple spider mites

[0153] This experiment was conducted in an apple orchard in Xiaoliu Village, south of Zhengzhou City, Henan Province. The apples were grown on a 15-year-old cultivar, Fujimu No. 1, with a row spacing of 2 m × 4 m and a height of approximately 2.5 m. The apple trees had been severely infested with leaf mites for several years. Field surveys and indoor microscopic examinations confirmed that the primary infesting mite species was the hawthorn spider mite. Applications were made on May 22, 2020 (sunny, temperature 21°C / 35°C).

[0154] The test drugs and control drugs are shown in the table below (Table 5).

[0155] Application method: Conventional application was used in the experiment. A backpack electric sprayer was used to evenly apply the pesticide to the entire fruit tree, spraying both the front and back of the leaves.

[0156] Experimental design: The experimental plot area was 2 fruit trees, and each treatment was replicated 4 times.

[0157] Survey Method: The test survey was conducted in accordance with GB / T 17980.7-2000. Base mite populations were measured before application, and active mite counts were measured 3, 7, and 14 days after application. Active mites were counted on five random leaves of each tree in each plot, located in each of the five directions: east, south, west, north, and center. Leaves were examined using a handheld magnifying glass, and the number of active mites was recorded.

[0158] Test data statistics:

[0159]

[0160] The test results are shown in the table below:

[0161] Table 5 Field efficacy test results of acaricidal composition against apple spider mites

[0162]

[0163] It can be seen from indoor toxicity tests and field efficacy tests that the acaricidal composition of the present invention exhibits good control effect on harmful mites and is safe to natural enemy insects.

[0164] Although the present invention has been described in detail above using general descriptions and specific implementation plans, it is obvious to those skilled in the art that some modifications or improvements can be made thereto based on the present invention. Therefore, these modifications or improvements made without departing from the spirit of the present invention shall fall within the scope of protection claimed by the present invention.

Claims

1. A mite-killing composition, characterized in that: The acaricidal composition comprises active ingredient A and active ingredient B, wherein the active ingredient A is a compound represented by formula I: The active ingredient B is clofentezine.

2. The acaricidal composition according to claim 1, characterized in that The mass ratio of the active ingredient A to the active ingredient B is 1:50 to 50:

1.

3. The acaricidal composition according to claim 1, characterized in that The active ingredient B is clofentezine, and the mass ratio of the active ingredient A to the active ingredient B is 1:42 to 36:

1.

4. The acaricidal composition according to claim 3, characterized in that The active ingredient B is clofentezine, and the mass ratio of the active ingredient A to the active ingredient B is 1:20 to 30:

1.

5. The acaricidal composition according to claim 1, characterized in that The acaricidal composition further comprises auxiliary components, which are carriers or adjuvants.

6. The acaricidal composition according to claim 1, characterized in that The total weight of the active ingredient A and the active ingredient B accounts for 0.5% to 80% of the total weight of the acaricidal composition.

7. The acaricidal composition according to claim 1, characterized in that The acaricidal composition can be prepared into a liquid preparation or a solid preparation. The liquid preparation is selected from a soluble solution, a soluble gel, an oil, a film-spreading oil, an emulsifiable concentrate, a latex, a dispersible liquid, an ointment, an aqueous emulsion, an oil emulsion, a microemulsion, a lipid suspension, a microcapsule suspension, an oil suspension, a dispersible oil suspension, a suspoemulsion, a microcapsule suspension-suspension, a microcapsule suspension-water emulsion or a microcapsule suspension-suspoemulsion; the solid preparation is selected from a powder, a granule, a pellet, a tablet, a strip, a wettable powder, an oil-dispersible powder, an emulsion powder, a water-dispersible granule, an emulsion granule, a water-dispersible tablet, a soluble powder, a soluble tablet or a soluble granule.

8. The acaricidal composition according to claim 7, characterized in that The preparation dosage form is suspension, emulsifiable concentrate, microemulsion, aqueous emulsion, dispersible oil suspension, water-dispersible granules or wettable powder.

9. Use of the acaricidal composition according to any one of claims 1 to 8 for preventing and controlling plant pests mites.

10. The use according to claim 9, characterized in that The plants are agricultural crops or forestry crops.

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