Anti-aging plant essence and application thereof

Abstract

The invention provides an anti-aging plant essence and application thereof, and belongs to the field of medical, dental or cosmetic preparations. The composition comprises the following components: tripeptide-1, palmitoyl pentapeptide-4, a turmeric root extract, a white plumeria rubra flower extract and an alchemilla japonica extract. The activity of the plant extract is innovatively improved through a biological fermentation process, and multi-dimensional coverage of an anti-aging effect is realized by combining a polypeptide targeted repair and plant extract multi-channel anti-oxidation and anti-inflammatory synergistic mechanism; meanwhile, the stability of the product is guaranteed by a freeze-dried powder dosage form and an anti-oxidation system, and an advanced solution with biological activity enhancement and user friendliness is provided for the development of anti-aging products.

Description

Technical Field

[0001] The present invention belongs to the field of medical, dental or cosmetic preparations, and in particular relates to an anti-aging plant essence and application thereof. Background Art

[0002] With the rapid development of science and technology and the continuous improvement of people's quality of life, people's demand for health and maintenance is growing. Oxidative stress, a mechanism closely related to various diseases and the aging process, has received widespread attention in recent years. The research and application of antioxidants has become a hot topic. Natural plant extracts, due to their rich diversity, minimal toxicity and side effects, and wide availability, have become a key research focus. With the in-depth study of natural plant chemical components, it has been discovered that many plant extracts are rich in antioxidant active substances, such as polyphenols and flavonoids. These substances have the ability to scavenge free radicals and inhibit oxidative reactions.

[0003] There is a direct and close causal relationship between antioxidants and skin anti-aging. Oxidative stress is a core driver of skin aging, and antioxidant mechanisms are a key line of defense against this process. Skin aging is the result of a multifactorial process, including oxidative damage, collagen loss, and inflammation. Therefore, achieving skin anti-aging effects requires a combination of multiple approaches. Summary of the Invention

[0004] The purpose of the present invention is to provide an anti-aging plant essence and its application, which achieves a relatively excellent anti-aging effect through multiple synergistic effects, including the following ingredients:

[0005] The following components are included in parts by mass:

[0006]

[0007] The turmeric root extract is obtained by purifying the product of turmeric root fermented with Lactobacillus rhamnosus;

[0008] The white frangipani flower extract is obtained by purifying the product of white frangipani flowers fermented by Aspergillus oryzae.

[0009] Preferably, the preparation method of the turmeric root extract comprises: mixing the activated Lactobacillus rhamnosus with the turmeric root fermentation substrate, then performing anaerobically fermentation, and after the fermentation is completed, centrifuging, concentrating, purifying, and drying;

[0010] The turmeric root fermentation substrate contains turmeric root, an auxiliary carbon source and water.

[0011] The conditions for anaerobic fermentation are:

[0012] 35-38℃, pH 6.0-7.0; ferment for at least 72h.

[0013] Preferably, the preparation method of the white frangipani flower extract comprises: mixing the activated Aspergillus oryzae with the white frangipani flower fermentation substrate, then aerobically fermenting the mixture in a solid-state manner, and after the fermentation is complete, centrifuging, concentrating, purifying, and drying the mixture;

[0014] The whitish frangipani flower fermentation substrate comprises whitish frangipani flowers, an auxiliary carbon source and water.

[0015] The conditions for aerobic solid-state fermentation are:

[0016] 28-32℃, pH 6.5-6.8; ferment for at least 60h.

[0017] Preferably, it further includes an antioxidant; the antioxidant is at least one of vitamin E, butylated hydroxytoluene, and idebenone; and the added amount of the antioxidant is 0.01-0.5.

[0018] Preferably, the dosage form of the anti-aging plant extract is freeze-dried powder.

[0019] When anti-aging plant extracts contain antioxidants, they also include lyoprotectants.

[0020] The present invention also provides the use of the aforementioned anti-aging plant extracts in the preparation of cosmetics, including but not limited to essences, creams, lotions, freeze-dried powders and other dosage forms.

[0021] The minimum addition amount of the aforementioned anti-aging plant extract in the preparation of cosmetics is 0.1 wt %.

[0022] The present invention uses multi-pathway synergistic anti-aging, mainly including:

[0023] A combination of two signal peptides, tripeptide-1 and palmitoyl pentapeptide-4. Tripeptide-1, with a smaller molecular weight, can penetrate the dermis, activating fibroblasts to promote collagen and elastin synthesis, improving skin sagging and wrinkles. Palmitoyl pentapeptide, by mimicking collagen fragments, stimulates the regeneration of type I and type IV collagen. Because peptides have a very low effective concentration, only a small amount is needed to achieve significant results.

[0024] Feather extract inhibits elastase activity, reduces wrinkle formation, and soothes sensitive skin through anti-inflammatory effects.

[0025] White frangipani flower extract is rich in polyphenolic compounds and flavonoids, which can neutralize oxidative damage, delay skin aging, and promote collagen synthesis.

[0026] Turmeric root extract contains highly active curcumin, which inhibits inflammatory factors (TNF-α, IL-1β) and lipid peroxidation, and reduces photoaging damage.

[0027] To enhance the beneficial effects of white frangipani and turmeric root, this invention selects specific bacterial species for fermentation. White frangipani is fermented aerobically using Aspergillus oryzae, breaking down potentially allergenic alkaloids while releasing more active polysaccharides and flavonoids. Similarly, turmeric root is fermented using Lactobacillus rhamnosus to generate secondary metabolites to optimize its effects.

[0028] The present invention found that not all fermentations of bacterial strains can bring better beneficial effects, which depends on the characteristics of the fermentation bacterial strain, process control and target active ingredients; for example, if the target product happens to be a nutrient required for bacterial metabolism, it will lead to a decrease in beneficial effects; or the process adaptation errors such as temperature, oxygen, pH range, etc.

[0029] The beneficial technical effects of the present invention are at least as follows:

[0030] This invention innovatively enhances the activity of plant extracts through a bio-fermentation process, combines targeted peptide repair with the synergistic mechanism of multi-pathway antioxidant and anti-inflammatory effects of plant extracts, and achieves multi-dimensional coverage of anti-aging efficacy. At the same time, the freeze-dried powder dosage form and antioxidant system ensure product stability, providing an advanced solution for the development of anti-aging products that combines enhanced bioactivity with user-friendliness. DETAILED DESCRIPTION

[0031] In order to better understand the present invention, the present invention is further described below in conjunction with specific serial numbers, wherein the terms used in the serial numbers are for describing specific embodiments and do not constitute a limitation on the scope of protection of the present invention.

[0032] In the specific implementation manner, the experimental methods used are conventional methods unless otherwise specified, and the materials, reagents, etc. used are all commercially available unless otherwise specified.

[0033] Unless otherwise specified, percentages and % in the specific embodiments are by default percentages by mass.

[0034] The sources of some raw materials used in the present invention are shown in Table 1 below:

[0035] Table 1

[0036] name INCI name / Latin name Tripeptide-1 Tripeptide-1 Palmitoyl Pentapeptide-4 Palmitoyl Pentapeptide-4 Turmeric root extract Turmeric (CURCUMA LONGA) Root Extract White Plumeria Flower Extract Plumeria Alba Flower Extract Lactobacillus plantarum L. plantarum Lactobacillus rhamnosus L. rhamnosus Bacillus subtilis B. subtilis Aspergillus oryzae A.oryzae

[0037] Example 1

[0038] The preparation method of fermented white frangipani flower extract comprises the following steps:

[0039] S1: Take fresh white frangipani petals, remove the calyx and pedicel, rinse with clean water and drain; then break into pieces of 0.5-1 cm;

[0040] S2: Prepare the substrate according to the ratio in Table 2 and add an appropriate amount of sterile water to adjust the water content;

[0041] S3: Prepare the activated bacteria into 1×10 7 -1×10 8 CFU / mL suspension was inoculated at a ratio of 5% and then cultured according to the conditions in Table 2;

[0042] S4: After the culture is completed, the cells are inactivated and the supernatant is collected after high-speed centrifugation. The supernatant is filtered using a nanofiltration membrane (NF) to collect the filtrate, which is freeze-dried and then sealed for storage.

[0043] Table 2

[0044]

[0045] Example 2

[0046] The preparation method of fermented turmeric root extract comprises the following steps:

[0047] S1: Take fresh turmeric root, wash it, slice it (thickness ≤ 0.5 cm), dry it at 60°C until the moisture content is ≤ 10%, and then grind it through a 60-mesh sieve to obtain turmeric root powder.

[0048] S2: Prepare the substrate according to the ratio in Table 3 and add an appropriate amount of sterile water to adjust the water content;

[0049] S3: Prepare the activated bacteria into 1×10 7 -1×10 8 CFU / mL suspension was inoculated at a ratio of 5% and then cultured according to the conditions in Table 3;

[0050] S4: After the culture is completed, the cells are inactivated at 60°C and the supernatant is collected after high-speed centrifugation. The supernatant is first filtered through a microfiltration membrane (MF) to obtain the filtrate, and then filtered through a nanofiltration membrane (NF) to obtain the filtrate, freeze-dried, and then sealed for storage.

[0051] Table 3

[0052]

[0053]

[0054] Example 3

[0055] Antioxidant properties of plant extracts were tested; the test method is as follows:

[0056] DPPH methanol solution is violet and has a strong absorbance at 517nm. If it combines with the sample, the absorbance value at 517nm will be reduced, thereby judging the ability of the sample to scavenge DPPH free radicals.

[0057] The specific method is as follows:

[0058] (1) Take Example 1, Example 2 and commercially available samples, mix them with sterile water, and prepare an equal volume (2 mL) of the test solution with a concentration of 0.5% and 2×10 -4 mol / L DPPH solution was mixed (A1);

[0059] (2) Take an equal volume of anhydrous ethanol (solvent for the test substance) and 2×10 -4 mol / L DPPH solution and mix well (A2);

[0060] (3) Take an equal volume of anhydrous ethanol and mix it with the test solution (A3);

[0061] (4) After 40 minutes of reaction, measure the absorbance of tubes A1, A2, and A3 at 517 nm.

[0062] The formula for calculating the clearance rate is: Clearance rate (%) = [1-(A1-A3) / A2] × 100%

[0063] The test results are shown in Table 4.

[0064] Table 4 Antioxidant test results

[0065]

[0066] According to Table 4, the effects of different strains after fermentation are very different, and may even be lower than those of commercially available products.

[0067] Example 4

[0068] The preparation of the anti-aging composition comprises the following steps:

[0069] Weigh each raw material according to the mass parts recorded in Table 5 below; mix well and seal for storage.

[0070] Table 5 Anti-aging composition ratio

[0071]

[0072]

[0073] In Table 5, the fermented turmeric root extract was derived from the sample prepared in sequence number 2 in Example 2; the fermented frangipani flower extract was derived from the sample prepared in sequence number 4 in Example 1.

[0074] Example 5

[0075] The performance test of the anti-aging composition includes the following test contents:

[0076] The antioxidant performance test was conducted in the same manner as in Example 3, with a sample concentration of 0.5%. The results are shown in Table 6 below.

[0077] Table 6 Antioxidant test results

[0078]

[0079] According to the data in Table 6, the five raw materials, tripeptide-1, palmitoyl pentapeptide-4, fermented turmeric root extract, fermented white frangipani flower extract, and linalool extract, are ranked from large to small in terms of their ability to scavenge free radicals as follows: tripeptide-1 > fermented white frangipani flower extract > fermented turmeric root extract > linalool extract > palmitoyl pentapeptide-4.

[0080] To promote type I collagen test, the test method is as follows:

[0081] Elastic fibers, composed of elastin and microfibrils, are distributed throughout the dermis and subcutaneous tissue, contributing to the skin's elasticity. However, environmental factors such as ultraviolet radiation, pressure, and pollution promote the production of elastase in the body. Elastase, a member of the chymosin family, degrades elastin, causing loss of connective tissue in the epidermis, leading to skin aging, wrinkles, and photoaging. This study investigated porcine pancreatic elastase, using N-succinyl-alanine-alanine-alanine-p-nitroanilide (AAAPVN) as a substrate. Porcine pancreatic elastase hydrolyzes AAAPVN, and its hydrolysis products increase absorbance at a wavelength of 420 nm. This absorbance is measured using a microplate reader to evaluate the firming and anti-wrinkle efficacy of the test sample.

[0082] 1. Solution Preparation

[0083] (1) Sample concentration setting: The anti-aging composition prepared in Example 4 was selected, and the test concentration was 0.5%.

[0084] (2) Prepare Tris-HCl buffer (0.1 M pH = 8.0): Weigh 2.42 g of Tris into a beaker, add 200 mL of ultrapure water, and adjust the pH to 8.0 with concentrated HCl after complete dissolution.

[0085] (3) Preparation of positive control tea polyphenol solution (1 mg / ml, 0.1%): Weigh 5 mg of tea polyphenol and dissolve it in 5 mL of Tris-HCl buffer.

[0086] (4) Preparation of substrate solution AAAPVN (2 mM): Weigh 4.51 mg of N-succinyl-alanine-alanine-alanine-p-nitroaniline and dissolve it in 5 ml of Tris-HCl buffer.

[0087] (5) Prepare porcine pancreatic elastase solution (0.171 U / mL): Dissolve 280 μl of the solution in 10 ml of Tris-HCl buffer.

[0088] 2. Add sample

[0089] The experiment was divided into four groups: sample group, positive control group, blank control group, and model control group. Four replicate wells were set up for each group at the same concentration. The amount of each solution added is shown in Table 7.

[0090] Table 7 Experimental groups

[0091]

[0092] 3. Determination

[0093] The reaction was allowed to proceed at room temperature for 15 min, and the absorbance was measured at 420 nm using a microplate reader.

[0094] 4. Calculation of results

[0095]

[0096] Where: A0-average absorbance of blank control wells;

[0097] A1-average absorbance of sample wells;

[0098] A2 - average absorbance of model control wells.

[0099] 5. Statistics

[0100] Statistical analysis was performed using the SPSS 22.0 statistical software package. Descriptive statistics were compiled for the measured values in the test areas. Changes in values and differences between the control and sample groups were calculated and analyzed. If the test data showed a normal distribution, the independent T-test was used for statistical analysis; if the test data showed a non-normal distribution, the rank sum test was used for statistical analysis. Two-tailed tests were used for all statistical analysis, with an α level of 0.05.

[0101] 6. Result determination

[0102] Compared with the model control group, the absorbance of the sample group showed a significant positive difference, indicating that the tested sample had the effect of inhibiting porcine pancreatic elastase. Otherwise, there was no such effect.

[0103] 7. Experimental results are shown in Table 8.

[0104] Table 8 Porcine pancreatic elastase inhibition rate test results

[0105]

[0106] The samples in Table 8 are derived from the anti-aging composition prepared in Example 4.

[0107] Compared with the model control group, the positive control tea polyphenol elastase inhibition rate was 68.43%, and its absorbance was significantly different from that of the model group, proving that this experiment is effective.

[0108] Compared with the model control group, the absorbance of samples 1-10 was significantly different from that of the model control group (p<0.05), which proved that the above composition has an inhibitory effect on porcine pancreatic elastase and has a certain firming and anti-wrinkle effect.

[0109] Example 6

[0110] The preparation of anti-aging freeze-dried powder includes the following steps:

[0111] Weigh each raw material according to the mass parts recorded in Table 9 below; mix them evenly and send them into a freeze dryer for freeze drying; then seal and store.

[0112] The samples of each serial number in Table 9 were additionally added with lyoprotectants. For example, when vitamin E was added, trehalose was added; when idebenone was added, mannitol and collagen were added. Such lyoprotectants are not shown again in Table 9.

[0113] The fermented turmeric root extract and fermented white frangipani flower extract in Table 9 were freeze-dried in advance and did not need to be freeze-dried again. They were mixed evenly after the other ingredients were freeze-dried.

[0114] Table 9 Anti-aging freeze-dried powder ratio

[0115] Raw materials (mass parts) / serial number 1 2 3 Tripeptide-1 0.1 0.1 0.1 Palmitoyl Pentapeptide-4 0.04 0.04 0.04 Fermented Turmeric Root Extract 4.00 4.00 4.00 Fermented White Plumeria Flower Extract 2.0 2.0 2.0 Collard Extract 1.0 1.0 1.0 Vitamin E 0.01 0.00 0.00 Butylated hydroxytoluene 0.00 0.30 0.00 Idebenone 0.00 0.00 0.50

[0116] Example 7

[0117] The antioxidant performance of the anti-aging freeze-dried powder was tested using the same test method as in Example 3, with sample concentrations of 0.1%, 0.5%, and 2.5%. The results are shown in Table 10 below.

[0118] Table 10 Antioxidant test results

[0119]

[0120] The experimental results in Table 10 show that after preparation into freeze-dried powder, the additional added antioxidants and the type of antioxidants have little effect on the performance.

[0121] The above detailed description is a specific description of one feasible embodiment of the present invention. This embodiment is not intended to limit the patent scope of the present invention. Any equivalent implementation or modification that does not depart from the present invention should be included in the scope of the technical solution of the present invention.

Claims

1. An anti-aging plant essence, characterized in that: The following components are included in parts by mass: The turmeric root extract is obtained by purifying the product of turmeric root fermented with Lactobacillus rhamnosus; The white frangipani flower extract is obtained by purifying a product obtained by fermenting white frangipani flowers with Aspergillus oryzae.

2. The anti-aging plant extract according to claim 1, characterized in that The preparation method of the turmeric root extract comprises: mixing activated Lactobacillus rhamnosus with a turmeric root fermentation substrate, then performing anaerobically fermentation, and after the fermentation is completed, centrifugation, concentration, purification, and drying; The turmeric root fermentation substrate contains turmeric root, an auxiliary carbon source and water.

3. The anti-aging plant essence according to claim 2, characterized in that: In the preparation method of the turmeric root extract, the conditions for anaerobic fermentation are: 35-38℃, pH 6.0-7.0; ferment for at least 72h.

4. The anti-aging plant extract according to claim 1, characterized in that: The preparation method of the white frangipani flower extract comprises: mixing activated Aspergillus oryzae with a white frangipani flower fermentation substrate, then subjecting the mixture to aerobic solid-state fermentation, and after the fermentation is complete, subjecting the mixture to centrifugal separation, concentration and purification, and drying; The whitish frangipani flower fermentation substrate contains whitish frangipani flowers, an auxiliary carbon source and water.

5. The anti-aging plant essence according to claim 2, characterized in that: In the preparation method of the white frangipani flower extract, the conditions for aerobic solid-state fermentation are: 28-32℃, pH 6.5-6.8; ferment for at least 60h.

6. The anti-aging plant essence according to claim 1, characterized in that: The invention also includes an antioxidant; the antioxidant is at least one of vitamin E, butylated hydroxytoluene, and idebenone; and the added amount of the antioxidant is 0.01-0.

5.

7. The anti-aging plant extract according to claim 1, characterized in that: The dosage form of the anti-aging plant extract is freeze-dried powder.

8. The anti-aging plant extract according to claim 7, characterized in that: When anti-aging plant extracts contain antioxidants, they also include lyoprotectants.

9. Use of the anti-aging plant extract according to any one of claims 1 to 8 in the preparation of cosmetics.

10. The use in preparing cosmetics according to claim 9, characterized in that: The minimum addition amount is 0.1 wt%.

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