A method for cultivating garlic seedlings using aerial bulbs and a device therefor

Abstract

The application discloses a kind of to cultivate the method and its device of air-borne bulb to garlic seedling, belong to agricultural bioengineering technical field, after breaking dormancy with exogenous hormone solution, air-borne bulb is washed and disinfected by sterile water, sodium hypochlorite solution, sterile water, then is cultivated in culture medium solution to sprout after, obtain garlic seedling;The cultivation method of the application can break the dormancy of air-borne bulb by making exogenous hormone solution circulate and flow soak air-borne bulb, can be monitored in circulating liquid box exogenous hormone solution concentration to real-time control, by controlling the concentration of solution in liquid tank, the exogenous hormone solution in culture box always maintains the concentration range set, soak at equal concentration can better break the dormancy of air-borne bulb, improve the induction rate of air-borne bulb, and the waste of exogenous hormone solution is reduced by circulating device, can be used again, save the cost of large-scale cultivation.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of agricultural bioengineering, and particularly relates to a method for cultivating garlic seedlings by using aerial bulbs and a device thereof. BACKGROUND

[0002] Garlic is a plant of the Allium genus of the Liliaceae family. It is difficult to produce viable seeds because the flower buds in the total involucre of garlic do not develop well. Generally, garlic cloves are used for propagation. However, long-term asexual propagation using garlic cloves can easily lead to degeneration of garlic vigor, increase of viral diseases, and difficulty in maintaining yield and quality. Therefore, methods such as off-site seed replacement, establishment of breeding gardens, aerial bulb propagation, and cultivation of virus-free seedlings are used to purify and rejuvenate garlic.

[0003] Aerial bulbs are formed from scale buds in the top flower buds of garlic sprouts. They are genetically stable, have a large number, small volume, high germination rate, and are easy to operate, making them the preferred explant material for in vitro garlic virus-free seedling cultivation. However, like garlic cloves, aerial bulbs have a long dormancy period and need to be artificially disturbed to break dormancy. Exogenous hormone soaking can break the dormancy of garlic aerial bulbs, allowing them to grow and produce garlic seedlings.

[0004] During the cultivation of garlic seedlings, different concentrations of exogenous hormones are used to soak the aerial bulbs according to the variety of garlic. The aerial bulbs that have broken dormancy are transferred to the culture medium after sterilization to grow buds. The buds are removed when they reach a certain height, and garlic seedlings are obtained by cultivating aerial bulbs. The garlic seedlings are then transferred to soil culture, and the planting of garlic is completed.

[0005] Chinese patent application CN108040882A discloses a method for inducing garlic test-tube seedlings. The aerial bulbs are soaked in an exogenous hormone solution and then sterilized. The aerial bulbs are then cultured in a culture medium containing a plant growth regulator to obtain garlic test-tube seedlings with a high induction rate. However, when a large number of aerial bulbs are cultured in the culture medium, viruses and microorganisms in the culture medium will also rapidly multiply. If there are aerial bulbs with high virus content, it may lead to the entire batch of garlic test-tube seedlings being infected, affecting the cultivation efficiency of garlic test-tube seedlings.

[0006] Chinese patent application CN110786216A discloses a method for direct sowing of garlic aerial bulbs. After the aerial bulbs are induced to germinate by spraying a germination-inducing agent, they are sown on a woven belt and sown on a large scale using the woven belt. However, this method only uses a germination-inducing agent to induce the aerial bulbs to germinate, which has low efficiency, takes a long time, has low bud activity, and low survival rate. SUMMARY

[0007] The present application aims at solving how to cultivate garlic seedlings in large quantities by using aerial bulbs, and improving the sprouting rate and survival rate of the aerial bulbs, and provides a method and device for cultivating garlic seedlings by using aerial bulbs.

[0008] The present application can be realized by the following technical solutions.

[0009] A method for cultivating garlic seedlings by using aerial bulbs, comprising the following steps:

[0010] Step one, cultivate garlic sprouts in Hoagland nutrient solution, cut the flower bud when the flower bud is swollen, collect the aerial bulbs and lay them on the non-woven fabric in the culture box, add low-temperature and equal-concentration exogenous hormone solution into the culture box, immerse the aerial bulbs in the exogenous hormone solution, and keep the liquid level unchanged, pump out the exogenous hormone solution after 10-14 hours of immersion;

[0011] Step two, wash the exogenous hormone solution on the surface of the aerial bulbs with sterile water in the culture box, then immerse the aerial bulbs in sodium hypochlorite solution for disinfection, and then wash the aerial bulbs with sterile water;

[0012] Step three, add equal-concentration culture medium solution into the culture box, immerse the aerial bulbs in the culture medium solution, and keep the liquid level unchanged, the culture medium solution flowing out of the culture box is sterilized by a sterilization device and then recycled into the culture box, cultivate the aerial bulbs under light conditions until they sprout, and obtain garlic seedlings.

[0013] Further, the exogenous hormone solution in step one is 100-300 mg / L GA3 solution or 15-20 mg / L FL solution.

[0014] Further, the Hoagland nutrient solution further contains NAA, ABA, streptomycin, penicillin and cephalosporin, the concentration of the sodium hypochlorite solution in step two is 4-5 wt%, and the culture medium solution in step three is MS medium.

[0015] Further, the concentration of NAA in the Hoagland nutrient solution is 0.5-1.5 mg / L, the concentration of ABA is 0.5-1.5 mg / L, the concentration of streptomycin is 100-300 mg / L, the concentration of penicillin is 100-300 mg / L, and the concentration of cephalosporin is 100-300 mg / L; the MS medium contains 30 g sucrose / L and does not contain agar, and the pH value is 6.

[0016] A device for cultivating garlic seedlings by using aerial bulbs, comprising a box body, a circulating liquid box, a culture box and a circulating pump.

[0017] The circulating liquid box is arranged above the box body, a plurality of groups of culture boxes are arranged in an array in the box body, a circulating pump is arranged at the rear side of the box body, the culture boxes are arranged in a sliding mode in the box body, L-shaped water inlets and water outlets are arranged at the sliding positions of the culture boxes and the box body, a liquid inlet pipeline is arranged at the left side of the box body, a liquid outlet pipeline is arranged at the right side of the box body, the liquid inlet pipeline is communicated with the L-shaped water inlets of the culture boxes, the liquid outlet pipeline is communicated with the water outlets, the liquid outlet pipeline is communicated to the circulating liquid box through the circulating pump, and the circulating liquid box is communicated with the liquid inlet pipeline.

[0018] Further, a sliding groove is arranged in the box body, the culture boxes are arranged in the box body in a sliding mode through the sliding groove, a light device is arranged in the box body above the culture boxes, the liquid inlet pipeline is communicated with the L-shaped water inlets through a serpentine joint arranged in the box body, and the liquid outlet pipeline is directly communicated with the water outlets.

[0019] Further, a first liquid tank, a second liquid tank and a third liquid tank are arranged side by side in the circulating liquid box, a disinfection device is arranged outside the third liquid tank, a circulating inlet pipe is arranged on one side of the disinfection device, and a communication pipe is arranged on the other side of the disinfection device, the communication pipe being communicated with the disinfection device and the third liquid tank.

[0020] Further, the second liquid tank and the first liquid tank are each provided with a circulating inlet pipe, and the circulating inlet pipes are arranged at the same height and in parallel.

[0021] Further, the first liquid tank, the second liquid tank and the third liquid tank are each provided with a circulating outlet pipe below the circulating inlet pipe, and the circulating outlet pipes are arranged at the same height and in parallel.

[0022] Further, the liquid outlet pipeline is communicated with a pump water inlet pipe of the circulating pump, a pump water outlet pipe is communicated with the circulating inlet pipe through a threaded joint, and an elastic hose is arranged between the threaded joint and the pump water outlet pipe; the liquid inlet pipeline is communicated with the circulating outlet pipe through a threaded joint, and an elastic hose is arranged between the threaded joint and the liquid inlet pipeline.

[0023] Further, threads are arranged at the pipe openings of the circulating inlet pipe and the circulating outlet pipe, the threads are matched with the threaded joints, the threaded joints are fixed by being screwed with the threads, and the circulating inlet pipe and the circulating outlet pipe are each provided with a valve.

[0024] The beneficial effects of the present application are as follows:

[0025] 1. The cultivation method of the present application can realize real-time regulation and control by monitoring the concentration of the exogenous hormone solution in the circulating liquid box, and the exogenous hormone solution in the culture box can always maintain a set concentration range by regulating the solution concentration in the liquid tank, so that the exogenous hormone solution with the same concentration can better break the dormancy of the aerial bulb, improve the induction rate of the aerial bulb, promote the growth of the aerial bulb in the same culture box, reduce the waste of the exogenous hormone solution, realize the reuse, and save the cost of large-scale cultivation.

[0026] 2、The cultivation method of the present application can cultivate the germination of aerial bulbs by circulating the culture solution, reduce the influence of the aerial bulb individuals containing virus on the whole culture box, the circulating culture solution can take away the culture solution possibly containing virus, and after sterilization by the sterilization device, return to the circulating liquid box, greatly reduce the influence of part of the aerial bulb individuals on the whole culture box, and the circulating culture solution does not need to be solidified by agar, the culture medium containing agar may form stubborn blocks at the corners of the culture box, and the culture solution is easy to breed bacteria, thereby polluting the culture box, by the cultivation method of the present application, the pollution of the culture solution to the corners of the culture box can also be reduced, which saves the amount of culture solution and improves the cleaning effect of the culture box.

[0027] 3、The device of the present application can make the liquid in the circulating liquid box flow into the culture box by gravity, the liquid in the circulating liquid box flows to the liquid inlet pipeline through the circulating outlet pipe, the liquid inlet pipeline is connected to the serpentine joint in the device, the serpentine joint is connected to the L-shaped water inlet in the culture box, so that the liquid flows into the culture box, the liquid in the culture box flows to the liquid outlet pipeline through the water outlet, and the liquid outlet pipeline pumps the liquid to the circulating inlet pipe through the circulating pump, so as to realize the circulation of the liquid, and the concentration and temperature of the liquid in the culture box are controlled by controlling the concentration and temperature of the liquid in the circulating liquid box.

[0028] 4、The device of the present application can connect different circulating inlet pipes and circulating outlet pipes through the flexible hose and threaded joint, and according to the cultivation method of the present application, there is a sterilization step every time the circulating inlet pipe and the circulating outlet pipe are replaced, so that the sterile environment in the pipeline and the box can be maintained, and pollution can be reduced, the cultivation method of the present application does not need to take out the culture box, the door of the box contains transparent glass, which can well observe the growth height of the garlic seedlings in the culture box, when the height of the garlic seedlings in the culture box is flat with the height of the bottom of the upper culture box, the culture box can be taken out, the cultivation is completed, and the bacterial pollution possibly caused by transferring the aerial bulbs to different solutions in the traditional cultivation method is greatly reduced, and the cultivation yield and quality of the garlic seedlings are improved.

[0029] 5、The device of the present application can complete the whole cultivation process of the garlic seedlings, and the device of the present application can also complete the cleaning work after cultivation, which greatly saves the labor, equipment and financial cost, and is conducive to large-scale cultivation of garlic seedlings. BRIEF DESCRIPTION OF DRAWINGS

[0030] Figure 1 It is a schematic diagram of the overall structure of the device of the present application;

[0031] Figure 2 It is a schematic diagram of the internal structure of the device of the present application;

[0032] Figure 3 Schematic diagram of the back of the device of the present application;

[0033] Figure 4 Schematic diagram of the front of the device of the present application;

[0034] Figure 5 Schematic diagram of the structure of the culture box of the present application;

[0035] Figure 6 Result graph of the induction rate of aerial bulbs by different concentrations of GA3 solution in the culture method of the present application;

[0036] Figure 7 Result graph of the induction rate of aerial bulbs by different concentrations of FL solution in the culture method of the present application;

[0037] Figure 8 Result graph of the removal rate of onion yellow dwarf virus by the garlic seedlings obtained by the present application:

[0038] Figure 9 Result of RT-PCR detection of virus by the garlic seedlings obtained by the present application;

[0039] Among them, 1, 2, and 3 are the detection results of OYDV, LYSV, and GarCLV viruses in the virus-free seedlings, and 4, 5, and 6 are the detection results of OYDV, LYSV, and GarCLV viruses in the CK;

[0040] Figure 10 Result graph of the difference in physiological characteristics of the garlic seedlings obtained by the present application compared with conventional seedlings;

[0041] The conventional seedlings are aerial bulbs sown in an open field, covered with soil, watered, and covered with a mulch to obtain garlic seedlings.

[0042] In the figure: 1, box; 101, liquid inlet pipeline; 102, liquid outlet pipeline; 103, serpentine joint; 104, sliding chute; 2, circulating liquid box; 201, first liquid tank; 202, second liquid tank; 203, third liquid tank; 204, circulating outlet pipe; 3, disinfection device; 301, circulating inlet pipe; 302, communication pipe; 4, culture box; 401, L-shaped water inlet; 402, water outlet; 5, circulating pump; 501, pump water inlet pipe; 502, pump water outlet pipe; 503, flexible hose; 504, threaded joint. DETAILED DESCRIPTION

[0043] With reference to the accompanying drawings, the technical solutions in the embodiments of the present application will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present application, but not all the embodiments of the present application. Based on the embodiments in the present application, all the other embodiments obtained by those skilled in the art without creative efforts belong to the scope of the present application.

[0044] Embodiment 1: please refer to Figures 1-2 As shown in the figure, a device for cultivating garlic seedlings by using aerial bulbs, comprising a box 1, a circulating liquid box 2, a culture box 4 and a circulating pump 5.

[0045] The circulating liquid box 2 is arranged above the box 1, and a plurality of groups of culture boxes 4 are arranged in an array in the box 1. The circulating pump 5 is arranged at the rear side of the box 1. The culture box 4 is slidingly arranged in the box 1. The L-shaped water inlet 401 and the water outlet 402 are arranged at the sliding position of the culture box 4 and the box 1. The liquid inlet pipeline 101 is arranged at the left side of the box 1, and the liquid outlet pipeline 102 is arranged at the right side of the box 1. The L-shaped water inlet 401 of the culture box 4 is communicated with the liquid inlet pipeline 101. The water outlet 402 is communicated with the liquid outlet pipeline 102. The liquid outlet pipeline 102 is communicated to the circulating liquid box 2 through the circulating pump 5. The circulating liquid box 2 is communicated with the liquid inlet pipeline 101.

[0046] Please refer to Figures 2-5 As shown in the figure, a sliding groove 104 is arranged in the box 1. The culture box 4 is slidingly arranged in the box 1 through the sliding groove 104. The light device is arranged in the box 1 above the culture box 4. The liquid inlet pipeline 101 is communicated with the L-shaped water inlet 401 through the serpentine joint 103 arranged in the box 1. The liquid outlet pipeline 102 is directly communicated with the water outlet 402.

[0047] The first liquid tank 201, the second liquid tank 202 and the third liquid tank 203 are arranged side by side in the circulating liquid box 2. The disinfection device 3 is arranged outside the third liquid tank 203. The circulating inlet pipe 301 is arranged on one side of the disinfection device 3, and the communicating pipe 302 is arranged on the other side. The communicating pipe 302 communicates the disinfection device 3 and the third liquid tank 203. The circulating inlet pipe 301 is arranged on one side of the second liquid tank 202 and the first liquid tank 201. The circulating inlet pipes 301 are arranged at the same height and are arranged in parallel.

[0048] The circulating outlet pipe 204 is arranged below the first liquid tank 201, the second liquid tank 202 and the third liquid tank 203. The circulating outlet pipes 204 are arranged at the same height and are arranged in parallel.

[0049] The liquid outlet pipeline 102 is connected with the pump inlet water pipe 501 of the circulating pump 5, the pump outlet water pipe 502 is connected with the circulating inlet pipe 301 through the threaded joint 504, and the flexible hose 503 is arranged between the threaded joint 504 and the pump outlet water pipe 502; the liquid inlet pipeline 101 is connected with the circulating outlet pipe 204 through the threaded joint 504, and the flexible hose 503 is arranged between the threaded joint 504 and the liquid inlet pipeline 101.

[0050] The threaded joints 504 are matched with the threads arranged at the pipe openings of the circulating inlet pipe 301 and the circulating outlet pipe 204, the threaded joints 504 are fixed by screwing, and the circulating inlet pipe 301 and the circulating outlet pipe 204 are provided with valves.

[0051] Embodiment 2: A method for cultivating garlic seedlings by using aerial bulbs, comprising the following steps:

[0052] Step one: Cultivate the sprouts in the Hoagland nutrient solution at a density of 300 roots / m 2 , the length of the sprouts is 15 cm, the flower buds are cut off when the flower buds of the sprouts are expanded to obtain the aerial bulbs, the aerial bulbs are laid on the non-woven fabric in the culture box 4, the culture boxes 4 are arranged in the box 1 at intervals according to the height of the required sprouts, the first liquid tank 201 is added with a GA3 solution with a concentration of 100 mg / L and a temperature of 5 ℃, the liquid inlet pipeline 101 and the pump outlet water pipe 502 are connected with the first liquid tank 201, the GA3 solution is immersed in the culture boxes 4 and circulates for 10 h, and then the GA3 solution in the culture boxes 4 is pumped out.

[0053] Step two: sterile water is added in the second liquid tank 202, the liquid inlet pipeline 101 is connected with the second liquid tank 202, the pump outlet water pipe 502 is connected with the waste liquid tank, the aerial bulbs in the culture boxes 4 are washed with the sterile water, after the washing is completed, a 4wt% sodium hypochlorite solution is added in the second liquid tank 202, the sodium hypochlorite solution in the second liquid tank 202 is immersed in the culture boxes 4 to soak and disinfect the aerial bulbs for 15 min, then the sodium hypochlorite solution is pumped out, the sterile water is added in the second liquid tank 202 again, and the aerial bulbs in the culture boxes 4 are washed with the sterile water, after the washing is completed, the sterile water is pumped out.

[0054] Step three: the culture medium solution is added in the third liquid tank 203, the culture medium solution is the MS culture medium, the liquid inlet pipeline 101 is connected with the third liquid tank 203 and the disinfection device 3, the culture medium solution is immersed in the culture boxes 4 to circulate, the light device in the box 1 is turned on, the circulating culture medium solution is disinfected by the disinfection device 3 and then flows back to the third liquid tank 203, after the aerial bulbs in the culture boxes 4 germinate, the culture boxes 4 are taken out, and the garlic seedlings are obtained.

[0055] The NAA concentration in the Hoagland nutrient solution is 0.5 mg / L, the ABA concentration is 0.5 mg / L, the streptomycin concentration is 100 mg / L, the penicillin concentration is 100 mg / L, and the cephalosporin concentration is 100 mg / L; the MS medium contains 30 g sucrose / L and does not contain agar, and the pH value is 6.

[0056] The illumination condition of the illumination device is 2600 lx, 16 h / d, and the temperature is 25℃.

[0057] Embodiment 3: A method for cultivating garlic seedlings by using aerial bulbs, comprising the following steps:

[0058] Step one, cultivating scapes in the Hoagland nutrient solution at a density of 300 roots / m 2 The length of the scapes is 17.5 cm, the flower buds are cut off after the scapes are expanded, and the aerial bulbs are obtained; the aerial bulbs are laid on the non-woven fabric in the culture box 4, the culture box 4 is arrayed in the box body 1 at the required height of the sprouts, the first liquid tank 201 is added with a GA3 solution with a concentration of 200 mg / L and a temperature of 5℃, the liquid inlet pipeline 101 and the water outlet pipeline 502 are connected to the first liquid tank 201, the GA3 solution is immersed in the aerial bulbs in the culture box 4 and circulates for 12 h, and then the GA3 solution in the culture box 4 is pumped out.

[0059] Step two, sterile water is added to the second liquid tank 202, the liquid inlet pipeline 101 is connected to the second liquid tank 202, the water outlet pipeline 502 is connected to the waste liquid tank, the aerial bulbs in the culture box 4 are washed with the sterile water, after the washing is completed, a 4.5 wt% sodium hypochlorite solution is added to the second liquid tank 202, the sodium hypochlorite solution in the second liquid tank 202 is immersed in the aerial bulbs in the culture box 4 for 17.5 min for disinfection, then the sodium hypochlorite solution is pumped out, and then the sterile water is added to the second liquid tank 202 again, the aerial bulbs in the culture box 4 are washed with the sterile water, and after the washing is completed, the sterile water is pumped out.

[0060] Step three, a culture medium solution is added to the third liquid tank 203, the culture medium solution is MS medium, the liquid inlet pipeline 101 is connected to the third liquid tank 203 and the disinfection device 3, the culture medium solution is immersed in the aerial bulbs in the culture box 4 and circulates, the illumination device in the box body 1 is turned on, the circulating culture medium solution is disinfected by the disinfection device 3 and then flows back to the third liquid tank 203, and after the aerial bulbs in the culture box 4 germinate, the culture box 4 is taken out, and the garlic seedlings are obtained.

[0061] The concentration of NAA in the Hoagland nutrient solution is 1 mg / L, the concentration of ABA is 1 mg / L, the concentration of streptomycin is 200 mg / L, the concentration of penicillin is 200 mg / L, and the concentration of cephalosporin is 200 mg / L; the MS culture medium contains 30 g sucrose / L and does not contain agar, and the pH value is 6.

[0062] The illumination condition of the illumination device is 2600 lx, 16 h / d, and the temperature is 25℃.

[0063] Embodiment 4: A method for cultivating garlic seedlings by using aerial bulbs, comprising the following steps:

[0064] Step one, cultivating garlic sprouts in the Hoagland nutrient solution at a density of 300 roots / m 2 The length of the garlic sprouts is 20 cm, the flower buds are cut off after the garlic sprouts flower buds swell to obtain aerial bulbs, the aerial bulbs are laid on the non-woven fabric in the culture box 4, the culture box 4 is arrayed in the box body 1 at the required height of the bud body, the first liquid tank 201 is added with a GA3 solution with a concentration of 300 mg / L and a temperature of 6℃, the liquid inlet pipeline 101 and the pump outlet water pipe 502 are connected to the first liquid tank 201, the GA3 solution is immersed in the aerial bulbs in the culture box 4 and circulates for 14 h, and then the GA3 solution in the culture box 4 is pumped out.

[0065] Step two, sterile water is added to the second liquid tank 202, the liquid inlet pipeline 101 is connected to the second liquid tank 202, the pump outlet water pipe 502 is connected to the waste liquid tank, the aerial bulbs in the culture box 4 are washed with sterile water, after the washing is completed, a 5 wt% sodium hypochlorite solution is added to the second liquid tank 202, the sodium hypochlorite solution in the second liquid tank 202 is immersed in the aerial bulbs in the culture box 4 for sterilization for 20 min, then the sodium hypochlorite solution is pumped out, sterile water is added to the second liquid tank 202 again, and the aerial bulbs in the culture box 4 are washed with sterile water, after the washing is completed, the sterile water is pumped out.

[0066] Step three, a culture medium solution is added to the third liquid tank 203, the culture medium solution is MS culture medium, the liquid inlet pipeline 101 is connected to the third liquid tank 203 and the sterilization device 3, the culture medium solution is immersed in the aerial bulbs in the culture box 4 and circulates, the illumination device in the box body 1 is turned on, the circulating culture medium solution is sterilized by the sterilization device 3 and flows back to the third liquid tank 203, after the aerial bulbs in the culture box 4 germinate, the culture box 4 is taken out, and garlic seedlings are obtained.

[0067] The concentration of NAA in the Hoagland nutrient solution is 1.5 mg / L, the concentration of ABA is 1.5 mg / L, the concentration of streptomycin is 300 mg / L, the concentration of penicillin is 300 mg / L, and the concentration of cephalosporin is 300 mg / L; the MS culture medium contains 30 g sucrose / L and does not contain agar, and the pH value is 6.

[0068] The illumination condition of the illumination device is 2600lx, 16h / d, and the temperature is 25℃.

[0069] Example 5: The difference from Example 2 is that the exogenous hormone solution is a 15mg / L FL solution.

[0070] Example 6: The difference from Example 3 is that the exogenous hormone solution is a 17.5mg / L FL solution.

[0071] Example 7: The difference from Example 4 is that the exogenous hormone solution is a 20mg / L FL solution.

[0072] Example 8: A method for using a device for cultivating garlic seedlings by using aerial bulbs, comprising the following steps:

[0073] S1, lay the aerial bulbs on the non-woven fabric in the culture box 4, add the exogenous hormone solution in the first liquid tank 201, adjust the extension hose 503 on the liquid inlet pipeline 101, align the threaded joint 504 on the liquid inlet pipeline 101 with the circulating outlet pipe 204 on the first liquid tank 201, tighten the threaded joint 504 to connect the circulating outlet pipe 204 on the first liquid tank 201 with the liquid inlet pipeline 101, then adjust the extension hose 503 on the pump outlet pipe 502, align the threaded joint 504 on the pump outlet pipe 502 with the circulating inlet pipe 301 on the first liquid tank 201, tighten the threaded joint 504 to connect the circulating inlet pipe 301 on the first liquid tank 201 with the pump outlet pipe 502, after the exogenous hormone solution immerses the aerial bulbs in the culture box 4, turn on the circulating pump 5, control the pump power to keep the liquid level in the culture box 4 unchanged, and soak to break the dormancy of the aerial bulbs.

[0074] S2, close the valve of the circulating outlet pipe 204 on the first liquid tank 201, after the circulating pump 5 pumps out all the liquid in the culture box 4, adjust the threaded joint 504 and the extension hose 503 on the liquid inlet pipeline 101 to connect the liquid inlet pipeline 101 with the circulating outlet pipe 204 on the second liquid tank 202, add sterile water in the second liquid tank 202, connect the pump outlet pipe 502 to the waste liquid barrel, turn on the circulating pump 5 to clean the aerial bulbs in the culture box 4, after cleaning, add sodium hypochlorite solution in the second liquid tank 202, turn on the circulating pump 5 to disinfect the aerial bulbs in the culture box 4, after disinfection, add sterile water again in the second liquid tank 202, turn on the circulating pump 5 to clean the aerial bulbs in the culture box 4, and after cleaning, pump out all the liquid in the culture box 4 by the circulating pump 5.

[0075] S3, adjust the threaded joint 504 and the flexible hose 503 on the liquid inlet pipeline 101, so that the liquid inlet pipeline 101 is communicated with the circulating outlet pipe 204 on the third liquid tank 203, the pump outlet pipe 502 is communicated with the circulating inlet pipe 301 on the sterilization device 3, the medium solution is added in the third liquid tank 203, after the medium solution immerses the non-woven fabric, the circulating pump 5 is started, the pump power is controlled so that the liquid level in the culture box 4 remains unchanged, the light illumination device is turned on, the medium solution pumped back by the pump outlet pipe 502 is sterilized through the sterilization device 3, and then flows back to the third liquid tank 203 from the communicating pipe 302, and after the length of the aerial bulb reaches the preset height, the garlic seedlings are obtained.

[0076] S4, adjust the threaded joint 504 and the flexible hose 503 on the liquid inlet pipeline 101, so that the liquid inlet pipeline 101 is communicated with the circulating outlet pipe 204 on the second liquid tank 202, the sodium hypochlorite solution is added in the second liquid tank 202, the pump outlet pipe 502 is connected to the waste liquid barrel, the circulating pump 5 is started to sterilize the culture box 4 and the pipeline, after sterilization, sterile water is added in the second liquid tank 202, the circulating pump 5 is started to clean the culture box 4 and the pipeline, and after cleaning, S1 is repeated.

[0077] Principle of the application:

[0078] The cultivation method of the application can make the exogenous hormone solution circulate and flow to soak the aerial bulb, the non-woven fabric can reduce the influence of the flowing solution on the displacement of the aerial bulb, the concentration of the exogenous hormone solution in the circulating liquid box 2 can be monitored to realize real-time regulation and control, the concentration of the solution in the liquid tank is regulated to make the exogenous hormone solution in the culture box 4 always maintain a set concentration range, the dormancy of the aerial bulb can be better broken, the induction rate of the aerial bulb can be improved, the waste of the exogenous hormone solution is reduced by the circulating device, the exogenous hormone solution can be reused, and the cost of large-scale cultivation can be saved.

[0079] The cultivation method of the application can cultivate the germination of the aerial bulb by circulating and flowing the medium solution, the influence of the aerial bulb individual containing viruses on the aerial bulb individuals in the whole culture box 4 is reduced, the medium solution possibly containing viruses can be taken away by the circulating and flowing medium solution, and then returned to the circulating liquid box 2 after sterilization through the sterilization device 3, the influence of part of the aerial bulb individuals on the aerial bulb individuals in the whole culture box 4 is greatly reduced, and the circulating and flowing medium solution does not need to be solidified by agar, the medium containing agar can form stubborn blocks at the corners of the culture box 4, the medium solution is easy to breed bacteria, and thus the culture box 4 is polluted, through the cultivation method of the application, the pollution of the medium solution to the corners of the culture box 4 can be reduced, the use amount of the medium solution is saved, and the cleaning effect of the culture box 4 is improved.

[0080] The device of the present application makes the liquid in the circulating liquid box 2 flow into the culture box 4 by gravity, and the liquid in the circulating liquid box 2 flows to the liquid inlet pipe 101 through the circulating outlet pipe 204, the liquid inlet pipe 101 is communicated to the serpentine joint 103 in the device, the serpentine joint 103 is communicated to the L-shaped water inlet 401 in the culture box 4, so that the liquid flows into the culture box 4, and the liquid in the culture box 4 flows to the liquid outlet pipe 102 through the water outlet 402, the liquid outlet pipe 102 pumps the liquid to the circulating inlet pipe 301 through the circulating pump 5, realizes the circulation of the liquid, and controls the concentration and temperature of the liquid in the culture box 4 by controlling the concentration and temperature of the liquid in the circulating liquid box 2.

[0081] The device of the present application can connect different circulating inlet pipes 301 and circulating outlet pipes 204 through the flexible hose 503 and the threaded joint 504, and according to the culture method of the present application, there is a sterilization step every time the circulating inlet pipe 301 and the circulating outlet pipe 204 are replaced, which can maintain the sterile environment in the pipe and the box 1 and reduce pollution, and the culture method of the present application does not need to take out the culture box 4 at all, the door of the box 1 contains transparent glass, which can well observe the growth height of the garlic seedlings in the culture box 4, and when the height of the garlic seedlings in the culture box 4 is flush with the bottom of the upper culture box 4, the culture box 4 can be taken out, the culture is completed, and the bacterial pollution that may be caused when the aerial bulbs are transferred to different solutions in the traditional culture method is greatly reduced, and the cultivation yield and quality of the garlic seedlings are improved.

[0082] The device of the present application can complete the whole culture process of garlic seedlings, and the device of the present application can also complete the cleaning work after culture, which greatly saves manpower, equipment and financial cost, and is conducive to large-scale cultivation of garlic seedlings.

[0083] Although the embodiments of the present application have been shown and described, those skilled in the art can understand that various changes, modifications, replacements and variations can be made to the embodiments without departing from the principles and spirits of the present application.

Claims

1. A method for cultivating garlic seedlings using aerial bulbs, characterized by, It comprises the following steps: Step one: cultivate the garlic sprout in the Hoagland nutrient solution, cut the flower bud when the bud is swollen, collect the aerial bulb and lay it on the non-woven fabric in the culture box (4), add the low-temperature and equal-concentration exogenous hormone solution into the culture box (4) in circulation, immerse the aerial bulb in the exogenous hormone solution and keep the liquid level unchanged, pump out the exogenous hormone solution after 10-14 hours of soaking; Step two: wash the exogenous hormone solution on the surface of the aerial bulb with sterile water in the culture box (4), then soak the aerial bulb in sodium hypochlorite solution for disinfection, and then wash the aerial bulb with sterile water again; Step three: add the equal-concentration culture medium solution into the culture box (4) in circulation, immerse the aerial bulb in the culture medium solution and keep the liquid level unchanged, the culture medium solution flowing out of the culture box (4) is sterilized by the sterilization device (3) and then circulated into the culture box (4), cultivate the aerial bulb to germination under the light condition, and obtain the garlic seedling.

2. The method for cultivating garlic seedlings using aerial bulbs according to claim 1, wherein The exogenous hormone solution in step one is 100-300 mg / L GA3 solution or 15-20 mg / L FL solution, and the temperature of the exogenous hormone solution is 4-6℃; The Hoagland nutrient solution further comprises NAA, ABA, streptomycin, penicillin and cephalosporin, and the concentration of the sodium hypochlorite solution in step two is 4-5 wt%; the culture medium solution in step three is MS medium.

3. The method for cultivating garlic seedlings using aerial bulbs according to claim 2, wherein The concentration of NAA in the Hoagland nutrient solution is 0.5-1.5 mg / L, the concentration of ABA is 0.5-1.5 mg / L, the concentration of streptomycin is 100-300 mg / L, the concentration of penicillin is 100-300 mg / L, and the concentration of cephalosporin is 100-300 mg / L; the MS medium contains 30 g sucrose / L and does not contain agar, and the pH value is 6.

4. A device for cultivating garlic seedlings using aerial bulbils, characterized in that, It comprises a box body (1), a circulating liquid box (2), a culture box (4) and a circulating pump (5); The circulating liquid box (2) is arranged above the box body (1), a plurality of culture boxes (4) are arranged in an array in the box body (1), and the circulating pump (5) is arranged at the rear side of the box body (1); the culture box (4) is slidingly arranged in the box body (1), L-shaped water inlets (401) and water outlets (402) are arranged at the sliding part of the culture box (4) and the box body (1), a liquid inlet pipeline (101) is arranged at the left side of the box body (1), a liquid outlet pipeline (102) is arranged at the right side of the box body (1), the L-shaped water inlets (401) of the culture boxes (4) are communicated with the liquid inlet pipeline (101), the water outlets (402) are communicated with the liquid outlet pipeline (102), the liquid outlet pipeline (102) is communicated with the circulating liquid box (2) through the circulating pump (5), and the circulating liquid box (2) is communicated with the liquid inlet pipeline (101).

5. The device for cultivating garlic seedlings using aerial bulbs according to claim 4, wherein A sliding groove (104) is arranged in the box body (1), the culture box (4) is slidingly arranged in the box body (1) through the sliding groove (104), a light device is arranged above the culture box (4) in the box body (1), the liquid inlet pipeline (101) is communicated with the L-shaped water inlets (401) through a serpentine joint (103) arranged in the box body (1), and the liquid outlet pipeline (102) is directly communicated with the water outlets (402).

6. The device for cultivating garlic seedlings using aerial bulbs according to claim 4, wherein The first liquid tank (201), the second liquid tank (202) and the third liquid tank (203) are arranged side by side in the circulating liquid box (2), the third liquid tank (203) is externally provided with a disinfection device (3), one side of the disinfection device (3) is provided with a circulating inlet pipe (301), and the other side is provided with a communication pipe (302) which communicates the disinfection device (3) and the third liquid tank (203).

7. The device for cultivating garlic seedlings using aerial bulbs according to claim 6, wherein The second liquid tank (202) and the first liquid tank (201) are both provided with a circulating inlet pipe (301) on one side, and the circulating inlet pipes (301) are arranged at the same height and in parallel.

8. The device for cultivating garlic seedlings using aerial bulbs according to claim 7, wherein The first liquid tank (201), the second liquid tank (202) and the third liquid tank (203) are all provided with a circulating outlet pipe (204) below the circulating inlet pipe (301), and the circulating outlet pipes (204) are arranged at the same height and in parallel.

9. The device for cultivating garlic seedlings using aerial bulbs according to claim 4, wherein The liquid outlet pipeline (102) communicates with the pump inlet water pipe (501) of the circulating pump (5), the pump outlet water pipe (502) communicates with the circulating inlet pipe (301) through a threaded joint (504), and a flexible hose (503) is arranged between the threaded joint (504) and the pump outlet water pipe (502); the liquid inlet pipeline (101) communicates with the circulating outlet pipe (204) through a threaded joint (504), and a flexible hose (503) is arranged between the threaded joint (504) and the liquid inlet pipeline (101).

10. The device for cultivating garlic seedlings using aerial bulbs according to claim 9, wherein Threaded joints (504) are arranged at the pipe openings of the circulating inlet pipe (301) and the circulating outlet pipe (204), the threaded joints (504) are matched with the threaded joints (504), the threaded joints (504) are fixed by being screwed with the threads, and the circulating inlet pipe (301) and the circulating outlet pipe (204) are both provided with valves.

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