Method for improving stability of michelia alba extract
Through low-temperature drying pretreatment, supercritical CO2 extraction and chitosan-tea polyphenol stabilization treatment, the problem of loss and poor stability of active ingredients during the extraction of the lanyard essential oil is solved, and efficient and stable preparation of lanyard extract is achieved, which is suitable for daily chemical and pharmaceutical fields.
Patent Information
- Application Number
- CN202510857039.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-06-25
- Publication Date
- 2025-08-22
AI Technical Summary
During the extraction process of existing white orchid essential oil, high temperature drying leads to loss of active ingredients. Essential oils are prone to chemical changes during storage, and their extraction efficiency is low and their stability is poor, which limits their application in the fields of daily chemical and pharmaceuticals.
Low-temperature drying pretreatment combined with trehalose, glycerol and nanocellulose, combined with supercritical CO2 extraction and chitosan-tea polyphenol stabilization treatment, formed an eutectic system to protect essential oil components and improve extraction rate and stability.
It significantly improves the extraction rate and stability of the white lantern extract, extends the shelf life, enhances water solubility and antioxidant properties, and is suitable for large-scale industrial production.
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Figure CN120514766A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of white jasmine extract, and in particular to a method for improving the stability of white jasmine extract. Background Art
[0002] Bailan (Michelia saccharina) is an evergreen tree of the genus Michelia in the Magnoliaceae family. Widely distributed across southwest and southeast my country, it holds both ecological and economic value. As an important aromatic plant, its leaves and flowers are rich in volatile terpenoids, with linalool being the primary component (over 50% by weight), which exhibits significant antibacterial and antiviral properties and acts as a mosquito repellent.
[0003] Existing essential oil extraction technologies mainly include steam distillation, solvent extraction, and supercritical fluid extraction. While distillation offers the advantages of ease of operation and pollution-free operation, the high temperature process can easily cause degradation of heat-sensitive terpenes. Solvent extraction can retain heat-sensitive substances, but there is a risk of residual organic solvents. Supercritical CO2 extraction technology allows for low-temperature extraction, but the extraction efficiency is low. More critically, existing technologies often use high-temperature drying during raw material pretreatment, resulting in the loss of active plant extract components. Furthermore, they lack effective stabilization treatment processes, making essential oils susceptible to chemical changes such as linalool isomerization and ester hydrolysis during storage, seriously affecting product quality and shelf life.
[0004] White jasmine essential oil, with its rich pharmacological properties, has been used in daily chemical products and pharmaceuticals. However, the extraction process is currently susceptible to damage to the active ingredients and chemical components in the essential oil, making it difficult to guarantee the quality of the essential oil. This significantly limits its practical application. Furthermore, in actual production applications, its poor water solubility, volatility, and sensitivity to light, heat, and oxygen reduce its actual quality and lead to poor results, a problem that urgently needs to be addressed. Summary of the Invention
[0005] The purpose of the present invention is to solve the shortcomings of the prior art and to propose a method for improving the stability of the white orchid extract.
[0006] A method for improving the stability of a white jasmine extract comprises the following steps:
[0007] S1. Add trehalose, glycerol, and nanocellulose to water and stir evenly, add white orchid leaves, ultrasonicate at 25-35°C for 1-2 hours, cool to -40--50°C, purge with nitrogen during the cooling process, prefreeze for 10-20 hours, freeze-crush, and sieve to obtain a prefabricated material;
[0008] S2. Add water to the prefabricated material, mix well and let it stand for 1-2 hours, use supercritical CO2 extraction for 2-4 hours, the supercritical CO2 fluid temperature is 40-50°C, the CO2 flow rate is 15-22L / h, the extraction pressure is 20-25MPa, and after the extraction is completed, the volatile oil component is separated under reduced pressure and collected;
[0009] S3. Sodium chloride is added to the volatile oil component to break the emulsion, and an organic solvent is added and stirred for 1-2 hours. After standing, the organic layer is separated, dried, and the organic solvent is removed by vacuum rotary evaporation.
[0010] Preferably, in S1, the white orchid leaves are pre-washed and then dried with hot air at 40-45° C. to a moisture content of 6-8%.
[0011] Preferably, in S1, the mass ratio of trehalose, glycerol, nanocellulose and white orchid leaves is 1-2:1-2:1-5:20-30.
[0012] Preferably, in S1, the ultrasonic frequency is 40-50 kHz.
[0013] Preferably, in S1, during the process of cooling to -40 to -50°C, the cooling rate is 1-5°C / min.
[0014] Preferably, in S2, water is added to the prefabricated material with a material-liquid ratio of 1:10-15.
[0015] Preferably, the mass ratio of sodium chloride to the organic solvent in S3 is 0.01-0.1:10-20, and the mass ratio of the organic solvent in S3 to the glycerol in S1 is 10-20:1-2.
[0016] Preferably, in S3, the organic solvent is food grade ethyl acetate.
[0017] A high-stability Phellodendron chinense extract comprises the following raw materials in parts by mass: 5-10 parts of the Phellodendron chinense extract, 1-5 parts of chitosan, and 0.5-2.5 parts of tea polyphenols.
[0018] The preparation method of the above-mentioned high-stability white jasmine extract includes the following steps: adding chitosan to acetic acid-sodium acetate buffer and stirring evenly, adding tea polyphenols thereto under stirring, stirring at 40-50°C for 1-2 hours to obtain pretreated tea polyphenols; stirring the above-mentioned white jasmine extract and anhydrous ethanol evenly, ultrasonically dispersing for 5-10 minutes, and adding it dropwise to the pretreated tea polyphenols under stirring, and ultrasonically treating for 10-20 minutes after the addition is complete.
[0019] Preferably, the frequency of ultrasonic dispersion is 40-50 kHz.
[0020] Preferably, the ultrasonic treatment frequency is 70-90 kHz after the dropwise addition is complete.
[0021] Beneficial effects:
[0022] The present invention utilizes white orchid leaves that are pre-treated by low-temperature drying and then frozen in combination with trehalose, glycerol, and nanocellulose. Nanocellulose can promote the regular growth of ice crystals, while trehalose can effectively inhibit the damage of ice crystal growth to active ingredients, form a eutectic system with glycerol, reduce the size of ice crystals, and synergistically enhance the protective effect on essential oil components.
[0023] The invention can not only effectively improve the release degree of the extract while destroying the cell wall structure through ultracold pretreatment, but also effectively avoid the destruction of the extract, comprehensively improve the extraction rate, and make the extract excellent in stability.
[0024] The present invention not only increases the linalool content in white orchid leaves to above 1.15 mg / mL through freezing treatment followed by supercritical CO2 extraction, but also achieves high stability and repeatability. The preparation method of the present invention is simple and easy to implement, highly operational, and suitable for large-scale industrial production.
[0025] The present invention further performs a stabilization treatment on the white orchid extract, wherein tea polyphenols are natural plant extracts containing phenolic hydroxyl groups in their molecular structure, and linalool from the white orchid extract can self-assemble with tea polyphenols through hydrogen bonds and electrostatic interactions, and further cooperate with chitosan to form a complex through electrostatic interactions, thereby significantly improving the water solubility and antioxidant stability of the extract, effectively reducing the damage to the active ingredients by light, heat, and oxygen, and greatly extending the storage period. BRIEF DESCRIPTION OF THE DRAWINGS
[0026] Figure 1 It is a comparison chart of the extraction rates of Examples 1-5 and Comparative Example 1.
[0027] Figure 2 This is a comparison chart of the linalool content in the white jasmine extracts obtained in Example 5 and Comparative Example 1.
[0028] Figure 3 This is a comparison chart of the peak area ratios of linalool to n-octanol in the Phellodendron chinense extracts obtained in Example 5 and Comparative Example 1.
[0029] Figure 4 This is a comparison chart of the particle size and PDI coefficient of the high-stability white jasmine extract obtained in Example 8 and Comparative Example 2 at 0, 7, 14, 21, 28, and 35 days.
[0030] Figure 5 This is a comparison chart of the particle size and PDI coefficient of the high-stability white jasmine extract obtained in Example 8 and Comparative Example 2 at different temperatures. DETAILED DESCRIPTION
[0031] The present invention will be further explained below with reference to specific embodiments.
[0032] Example 1
[0033] A method for improving the stability of a white jasmine extract comprises the following steps:
[0034] S1. Wash the white orchid leaves, dry them in the air, and dry them with hot air at 40°C until the moisture content of the leaves is 6%.
[0035] 1 g of trehalose, 1 g of glycerol, and 1 g of nanocellulose were added to 40 g of deionized water and stirred evenly. 20 g of the dried white orchid leaves were added thereto, and the mixture was ultrasonically treated at 25°C for 1 h with an ultrasonic frequency of 40 kHz. The mixture was cooled to -40°C at a rate of 1°C / min, and nitrogen was purged during the cooling process. The mixture was pre-frozen for 10 h, crushed in a freezer crusher, and passed through an 80-mesh sieve to obtain a prefabricated material.
[0036] S2, deionized water was added to the prefabricated material at a material-liquid ratio of 1:10, the mixture was evenly mixed and allowed to stand for 1 hour, and the mixture was transferred to an extraction tank and extracted with supercritical CO2 for 2 hours. The supercritical CO2 fluid temperature was 40°C, the CO2 flow rate was 15L / h, and the extraction pressure was 20MPa. After the extraction was completed, the extract was separated by decompression in a separation kettle, and the volatile oil component was collected;
[0037] S3. Add 0.01 g of sodium chloride to the volatile oil component to break the emulsion, add 10 g of food-grade ethyl acetate and stir for 1 hour. After standing, separate the organic layer, dry it with anhydrous sodium sulfate, and remove the ethyl acetate by vacuum rotary evaporation.
[0038] Example 2
[0039] A method for improving the stability of a white jasmine extract comprises the following steps:
[0040] S1. Wash the white orchid leaves, dry them in the air, and dry them with hot air at 45°C until the moisture content of the leaves is 8%.
[0041] 2 g of trehalose, 2 g of glycerol, and 5 g of nanocellulose were added to 60 g of deionized water and stirred evenly. 30 g of the dried white orchid leaves were added thereto, and the mixture was ultrasonically treated at 35 ° C for 2 h with an ultrasonic frequency of 50 kHz. The mixture was cooled to -50 ° C at a rate of 5 ° C / min, and nitrogen was purged during the cooling process. The mixture was pre-frozen for 20 h, crushed in a freezer crusher, and passed through an 80-mesh sieve to obtain a prefabricated material.
[0042] S2, deionized water was added to the prefabricated material at a material-liquid ratio of 1:15, the mixture was evenly mixed and allowed to stand for 2 hours, and the mixture was transferred to an extraction tank and extracted with supercritical CO2 for 4 hours. The supercritical CO2 fluid temperature was 50°C, the CO2 flow rate was 22L / h, and the extraction pressure was 25MPa. After the extraction was completed, the extract was separated by decompression in a separation kettle, and the volatile oil component was collected;
[0043] S3. Add 0.1 g of sodium chloride to the volatile oil component to break the emulsion, add 20 g of food-grade ethyl acetate and stir for 2 h. After standing, separate the organic layer, dry it with anhydrous sodium sulfate, and remove the ethyl acetate by vacuum rotary evaporation.
[0044] Example 3
[0045] A method for improving the stability of a white jasmine extract comprises the following steps:
[0046] S1. Wash the white orchid leaves, dry them in the air, and dry them with hot air at 41°C until the moisture content of the leaves is 7.5%;
[0047] 1.2 g of trehalose, 1.7 g of glycerol, and 2 g of nanocellulose were added to 55 g of deionized water and stirred evenly. 22 g of the dried white orchid leaves were added thereto, and the mixture was ultrasonically treated at 32° C. for 80 min at an ultrasonic frequency of 48 kHz. The mixture was cooled to -48° C. at a rate of 2° C. / min, and nitrogen was purged during the cooling process. The mixture was pre-frozen for 12 h, crushed in a freezer crusher, and passed through an 80-mesh sieve to obtain a prefabricated material.
[0048] S2, deionized water was added to the prefabricated material at a material-liquid ratio of 1:13, the mixture was evenly mixed and allowed to stand for 80 min, and the material was transferred to an extraction tank and extracted with supercritical CO2 for 3.5 h. The supercritical CO2 fluid temperature was 42 ° C, the CO2 flow rate was 20 L / h, and the extraction pressure was 21 MPa. After the extraction was completed, the extract was separated by decompression in a separation kettle, and the volatile oil component was collected;
[0049] S3. Add 0.02 g of sodium chloride to the volatile oil component to break the emulsion, add 18 g of food-grade ethyl acetate and stir for 80 min. After standing, separate the organic layer, dry it with anhydrous sodium sulfate, and remove the ethyl acetate by vacuum rotary evaporation.
[0050] Example 4
[0051] A method for improving the stability of a white jasmine extract comprises the following steps:
[0052] S1. Wash the white orchid leaves, dry them in the air, and dry them with hot air at 43°C until the moisture content of the leaves is 6.5%;
[0053] 1.8 g of trehalose, 1.3 g of glycerol, and 4 g of nanocellulose were added to 45 g of deionized water and stirred evenly. 28 g of the dried white orchid leaves were added thereto, and ultrasonic treatment was performed at 28° C. for 100 min at an ultrasonic frequency of 42 kHz. The temperature was then cooled to -42° C. at a rate of 4° C. / min. The temperature was purged with nitrogen during the cooling process. The mixture was pre-frozen for 18 h, crushed in a freezer crusher, and passed through an 80-mesh sieve to obtain a prefabricated material.
[0054] S2, deionized water was added to the prefabricated material at a material-liquid ratio of 1:11, the mixture was evenly mixed and allowed to stand for 100 min, and the mixture was transferred to an extraction tank and extracted with supercritical CO2 for 2.5 h. The supercritical CO2 fluid temperature was 48 ° C, the CO2 flow rate was 16 L / h, and the extraction pressure was 23 MPa. After the extraction was completed, the extract was separated by decompression in a separation kettle, and the volatile oil component was collected;
[0055] S3. Add 0.08 g of sodium chloride to the volatile oil component to break the emulsion, add 12 g of food-grade ethyl acetate and stir for 100 min. After standing, separate the organic layer, dry it with anhydrous sodium sulfate, and remove the ethyl acetate by vacuum rotary evaporation.
[0056] Example 5
[0057] A method for improving the stability of a white jasmine extract comprises the following steps:
[0058] S1. Wash the white orchid leaves, dry them in the air, and dry them with hot air at 42°C until the moisture content of the leaves is 7%.
[0059] 1.5 g of trehalose, 1.5 g of glycerol, and 3 g of nanocellulose were added to 50 g of deionized water and stirred evenly. 25 g of the dried white orchid leaves were added thereto, and the mixture was ultrasonically treated at 30° C. for 90 min at an ultrasonic frequency of 45 kHz. The mixture was cooled to -45° C. at a rate of 3° C. / min, and nitrogen was purged during the cooling process. The mixture was pre-frozen for 15 h, crushed in a freezer crusher, and passed through an 80-mesh sieve to obtain a prefabricated material.
[0060] S2, deionized water was added to the prefabricated material at a material-liquid ratio of 1:12, the mixture was evenly mixed and allowed to stand for 90 min, and the mixture was transferred to an extraction tank and extracted with supercritical CO2 for 3 h. The supercritical CO2 fluid temperature was 45 ° C, the CO2 flow rate was 18 L / h, and the extraction pressure was 22 MPa. After the extraction was completed, the extract was separated by decompression in a separation kettle, and the volatile oil component was collected;
[0061] S3. Add 0.05 g of sodium chloride to the volatile oil component to break the emulsion, add 15 g of food-grade ethyl acetate and stir for 90 min. After standing, separate the organic layer, dry it with anhydrous sodium sulfate, and remove the ethyl acetate by vacuum rotary evaporation.
[0062] Comparative Example 1
[0063] A method for improving the stability of a white jasmine extract comprises the following steps:
[0064] S1. Wash the white orchid leaves, dry them in the air, and dry them with hot air at 42°C until the moisture content of the leaves is 7%.
[0065] 3 g of glycerol and 3 g of nanocellulose were added to 50 g of deionized water and stirred evenly. 25 g of the dried white orchid leaves were added thereto, and ultrasonic treatment was performed at 30° C. for 90 min at an ultrasonic frequency of 45 kHz. The temperature was then cooled to -45° C. at a rate of 3° C. / min. Nitrogen was purged during the cooling process, and the mixture was pre-frozen for 15 h. The mixture was crushed in a freezer crusher and passed through an 80-mesh sieve to obtain a prefabricated material.
[0066] S2, deionized water was added to the prefabricated material at a material-liquid ratio of 1:12, the mixture was evenly mixed and allowed to stand for 90 min, and the mixture was transferred to an extraction tank and extracted with supercritical CO2 for 3 h. The supercritical CO2 fluid temperature was 45 ° C, the CO2 flow rate was 18 L / h, and the extraction pressure was 22 MPa. After the extraction was completed, the extract was separated by decompression in a separation kettle, and the volatile oil component was collected;
[0067] S3. Add 0.05 g of sodium chloride to the volatile oil component to break the emulsion, add 15 g of food-grade ethyl acetate and stir for 90 min. After standing, separate the organic layer, dry it with anhydrous sodium sulfate, and remove the ethyl acetate by vacuum rotary evaporation.
[0068] The extraction rates of Examples 1-5 and Comparative Example 1 were measured. Figure 1 As shown, the extraction rates of Examples 1-5 were significantly higher than those of Comparative Example 1 (P < 0.05); while the extraction rate of Example 5 was the highest, but there was no significant difference between it and Examples 1-4.
[0069] Gas chromatography was used to determine the linalool content of the white jasmine extracts obtained in Example 5 and Comparative Example 1. Chromatographic column: DB-5MS (30m×0.25mm×0.25m) from Agilent Technologies, USA. The injection port temperature was 250°C, 1 μL was injected each time, the split ratio was 50:1, the carrier gas was helium, and the flow rate was 1.0 mL / min. Temperature program: maintain at 40°C for 2 minutes, then increase to 130°C at a rate of 3°C / min, maintain for 5 minutes, and then increase to 250°C at a rate of 5°C / min, maintain for 5 minutes. Figure 2 As shown, the content of linalool in the P. chinensis extract obtained in Example 5 is significantly higher than that in Comparative Example 1.
[0070] Take 0.1 mL of the white jasmine extract obtained in Example 5 and Comparative Example 1, add 0.1 mL of 9.6 mg / mL n-octanol ethyl acetate solution to each, place in a 2 mL volumetric flask, and dilute to volume with ethyl acetate to prepare a test solution. According to the above-mentioned gas chromatography analysis method, the sample was injected and measured after 0, 1, 4, 6, 12, and 24 hours, and the peak areas of linalool and n-octanol were recorded. The peak area ratio of linalool to n-octanol was calculated. Figure 3As shown, the peak area ratio of linalool to n-octanol in the white jasmine extract obtained in Example 5 is substantially stable at 2, while the peak area ratio of linalool to n-octanol in the white jasmine extract obtained in Comparative Example 1 continues to decrease after 6 hours, confirming that the white jasmine extract obtained by the method of the present invention is more stable.
[0071] The reason for these results is that the present invention utilizes white orchid leaves pretreated with low-temperature drying and then freeze-treated with trehalose, glycerol, and nanocellulose. Nanocellulose promotes the regular growth of ice crystals, while trehalose effectively inhibits ice crystal growth and damage to active ingredients. Trehalose, in combination with glycerol, forms a eutectic system and reduces ice crystal size, synergistically enhancing the protective effect of essential oil components. While ultracold pretreatment disrupts cell wall structure, the present invention not only effectively increases the release of the extract but also effectively avoids damage to the extract, comprehensively improving the extraction rate and enhancing the stability of the extract.
[0072] Example 6
[0073] A high-stability Phellodendron chinense extract, whose raw materials include: 5g of Phellodendron chinense extract obtained in Example 5, 1g of chitosan, and 0.5g of tea polyphenols.
[0074] The preparation method of the above-mentioned high-stability white jasmine extract includes the following steps: adding chitosan to 50g of acetic acid-sodium acetate buffer (pH=4.5) and stirring evenly, adding tea polyphenols thereto under stirring, and stirring at a temperature of 40°C for 1h to obtain pretreated tea polyphenols; stirring the above-mentioned white jasmine extract and 5g of anhydrous ethanol evenly, ultrasonically dispersing for 5min at an ultrasonic frequency of 40kHz, and adding the mixture dropwise to the pretreated tea polyphenols under stirring at a stirring speed of 1000r / min. After the addition is complete, ultrasonic treatment is performed for 10min at an ultrasonic frequency of 70kHz.
[0075] Example 7
[0076] A high-stability Phellodendron chinense extract, whose raw materials include: 10g of Phellodendron chinense extract obtained in Example 5, 5g of chitosan, and 2.5g of tea polyphenols.
[0077] The preparation method of the above-mentioned high-stability white jasmine extract includes the following steps: adding chitosan to 100g of acetic acid-sodium acetate buffer (pH=4.5) and stirring evenly, adding tea polyphenols thereto under stirring, and stirring at a temperature of 50°C for 2h to obtain pretreated tea polyphenols; stirring the above-mentioned white jasmine extract and 10g of anhydrous ethanol evenly, ultrasonically dispersing for 10min at an ultrasonic frequency of 50kHz, and adding the mixture dropwise to the pretreated tea polyphenols under stirring at a stirring speed of 1500r / min. After the addition is complete, ultrasonic treatment is performed for 20min at an ultrasonic frequency of 90kHz.
[0078] Example 8
[0079] A high-stability Phellodendron chinense extract, whose raw materials include: 7.5g of Phellodendron chinense extract obtained in Example 5, 3g of chitosan, and 1.5g of tea polyphenols.
[0080] The preparation method of the above-mentioned high-stability white jasmine extract includes the following steps: adding chitosan to 75g of acetic acid-sodium acetate buffer (pH=4.5) and stirring evenly, adding tea polyphenols thereto under stirring, and stirring at a temperature of 45°C for 90 minutes to obtain pretreated tea polyphenols; stirring the above-mentioned white jasmine extract and 8g of anhydrous ethanol evenly, ultrasonically dispersing for 8 minutes at an ultrasonic frequency of 45kHz, and adding the mixture dropwise to the pretreated tea polyphenols under stirring at a stirring speed of 1200r / min. After the addition is complete, ultrasonic treatment is performed for 15 minutes at an ultrasonic frequency of 80kHz.
[0081] Comparative Example 2
[0082] A high-stability Phellodendron chinense extract, whose raw materials include: 7.5g of Phellodendron chinense extract obtained in Example 5 and 1.5g of tea polyphenols.
[0083] The preparation method of the above-mentioned high-stability white jasmine extract includes the following steps: adding tea polyphenols to 75g of acetic acid-sodium acetate buffer (pH=4.5), stirring at a temperature of 45°C for 90 minutes to obtain pretreated tea polyphenols; stirring the above-mentioned white jasmine extract and 8g of anhydrous ethanol evenly, ultrasonically dispersing for 8 minutes at an ultrasonic frequency of 45kHz, and adding the mixture dropwise to the stirred pretreated tea polyphenols at a stirring speed of 1200r / min. After the addition is complete, ultrasonic treatment is performed for 15 minutes at an ultrasonic frequency of 80kHz.
[0084] The particle size and PDI coefficient of the high stability white orchid extract obtained in Example 8 and Comparative Example 2 were measured on days 0, 7, 14, 21, 28 and 35. Figure 4 As shown, the particle size and PDI coefficient of the high-stability white orchid extract obtained in Example 8 are more stable.
[0085] The high-stability white orchid extracts obtained in Example 8 and Comparative Example 2 were placed in a refrigerator and stored at -18°C and 5°C for 12 hours, respectively. They were then heated in a water bath at 40, 50, 60, 70, 80, and 90°C for 30 minutes, and their average particle sizes and PDI coefficients were measured. Figure 5 As shown, the particle size and PDI coefficient of the high-stability white orchid extract obtained in Example 8 are more stable.
[0086] The reason for the above results is that the present invention further performs a stabilization treatment on the white orchid extract, wherein tea polyphenols are natural plant extracts, and their molecular structure contains phenolic hydroxyl groups, and linalool from the white orchid extract can self-assemble with tea polyphenols through hydrogen bonds and electrostatic effects, and further cooperate with chitosan to form a complex through electrostatic effects, which significantly improves the water solubility and antioxidant stability of the extract, effectively reduces the damage to the active ingredients by light, heat, and oxygen, and greatly extends the storage period.
[0087] The above description is only a preferred specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any technician familiar with the technical field, within the technical scope disclosed by the present invention, who makes equivalent replacements or changes based on the technical solution and inventive concept of the present invention, should be covered by the scope of protection of the present invention.
Claims
1. A method for improving the stability of a white jasmine extract, characterized in that: The steps include: S1. Add trehalose, glycerol, and nanocellulose to water and stir evenly, add white orchid leaves, ultrasonicate at 25-35°C for 1-2 hours, cool to -40--50°C, purge with nitrogen during the cooling process, prefreeze for 10-20 hours, freeze-crush, and sieve to obtain a prefabricated material; S2. Add water to the prefabricated material, mix well and let it stand for 1-2 hours, use supercritical CO2 extraction for 2-4 hours, the supercritical CO2 fluid temperature is 40-50°C, the CO2 flow rate is 15-22L / h, the extraction pressure is 20-25MPa, and after the extraction is completed, the volatile oil component is separated under reduced pressure and collected; S3. Sodium chloride is added to the volatile oil component to break the emulsion, and an organic solvent is added and stirred for 1-2 hours. After standing, the organic layer is separated, dried, and the organic solvent is removed by vacuum rotary evaporation.
2. The method for improving the stability of the white angelica extract according to claim 1, characterized in that: In S1, the water content of the white orchid leaves is 6-8%, and the mass ratio of trehalose, glycerol, nanocellulose, and white orchid leaves is 1-2:1-2:1-5:20-30.
3. The method for improving the stability of the white angelica extract according to claim 1, characterized in that: In S1, the ultrasound frequency is 40-50 kHz.
4. The method for improving the stability of the white angelica extract according to claim 1, characterized in that: In S1, during the process of cooling to -40 to -50°C, the cooling rate is 1-5°C / min.
5. The method for improving the stability of the white angelica extract according to claim 1, characterized in that: In S2, water is added to the prefabricated material with a material-to-liquid ratio of 1:10-15.
6. The method for improving the stability of the white angelica extract according to claim 1, characterized in that: The mass ratio of sodium chloride to the organic solvent in S3 is 0.01-0.1:10-20, and the mass ratio of the organic solvent in S3 to the glycerol in S1 is 10-20:1-2.
7. The method for improving the stability of the white angelica extract according to claim 1, characterized in that: In S3, the organic solvent is food grade ethyl acetate.
8. A high-stability white orchid extract, characterized in that: The raw materials include, by mass: 5-10 parts of the white jasmine extract obtained by the method for improving the stability of the white jasmine extract according to any one of claims 1 to 7, 1-5 parts of chitosan, and 0.5-2.5 parts of tea polyphenols.
9. A method for preparing the high-stability white jasmine extract according to claim 8, characterized in that: The method comprises the following steps: adding chitosan to an acetic acid-sodium acetate buffer solution and stirring evenly, adding tea polyphenols thereto in a stirring state, and stirring at 40-50° C. for 1-2 hours to obtain pretreated tea polyphenols; uniformly stirring the P. glabra extract obtained by the method for improving the stability of the P. glabra extract according to any one of claims 1 to 7 with anhydrous ethanol, performing ultrasonic dispersion for 5-10 minutes, and dripping the mixture into the pretreated tea polyphenols in a stirring state, and ultrasonically treating the mixture for 10-20 minutes after the dripping is complete.
10. The method for preparing the high-stability Phellodendron chinense extract according to claim 9, characterized in that: The frequency of ultrasonic dispersion is 40-50 kHz; after the dropwise addition is complete, the frequency of ultrasonic treatment is 70-90 kHz.