Method for preparing organic protein base material for feed from chicken manure subjected to spectrum treatment through synthetic biotechnology

By spectral treatment of chicken manure and microbial fermentation, the carbon-nitrogen ratio is adjusted, and organic protein base materials are prepared using Candida prion and Lactobacillus plantarum, the problems of low protein content and large ammonia emissions in chicken manure treatment are solved, and efficient utilization and environmentally friendly treatment are achieved.

CN120531055APending Publication Date: 2025-08-26WEINONG XINKE (LIAONING) EQUIPMENT MANUFACTURING CO LTD
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Patent Information

Application Number
CN202510762173.5
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-06-09
Publication Date
2025-08-26

AI Technical Summary

Technical Problem

In the prior art, chicken manure treatment has problems such as low protein content, low nitrogen utilization, large ammonia emissions, resulting in waste of resources and environmental pollution.

Method used

After spectroscopic treatment of chicken manure, the carbon-nitrogen ratio was adjusted to (20-30): 1, and mixed Candida and Lactobacillus plantarum for stacking and fermenting to prepare organic protein base.

Benefits of technology

Significantly increase the protein content in chicken manure, reduce ammonia emissions, shorten the fermentation time, and improve the efficiency of chicken manure preparation feed. The prepared organic protein base material meets livestock and poultry feed standards, can replace soybean meal, and is low in cost.

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Abstract

The invention provides a method for preparing a feed organic protein base material from chicken manure subjected to spectrum treatment through a synthetic biotechnology, and belongs to the technical field of resource utilization. The invention provides a preparation method of an organic protein base material, which comprises the following steps: carrying out spectrum treatment on chicken manure to obtain chicken manure subjected to spectrum treatment; adjusting the carbon-nitrogen ratio of the chicken manure subjected to spectrum treatment to (20-30): 1 to obtain a compost base material; mixing the compost base material with a microbial agent, and carrying out stacking fermentation to obtain an organic protein base material; the microbial agent comprises candida utilis and lactobacillus plantarum. According to the preparation method provided by the invention, the protein content in the chicken manure can be remarkably increased, and the ammonia emission in the chicken manure fermentation process is reduced. The content of protein and essential amino acid in the organic protein base material prepared by the preparation method is high, the organic protein base material contains probiotics, nutritional indexes and safety indexes meet feed standards, and the organic protein base material can be used for replacing soybean meal to be applied as livestock and poultry feed.
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Description

Technical Field

[0001] The invention belongs to the technical field of resource utilization, and particularly relates to a method for preparing an organic protein base material for feed from spectrally treated chicken manure by using synthetic biology. Background Art

[0002] Chicken manure is rich in nutrients such as protein and minerals, but there are many problems with its direct use without treatment. Traditional methods of treating chicken manure are mostly simple composting or discarding, which not only wastes resources but also pollutes the environment. At the same time, with the development of animal husbandry, feed costs continue to rise, and finding low-cost, high-nutrition feed raw materials has become the key to the development of the industry. In this context, it is very necessary to develop a technology for using chicken manure to make baked organic protein base for feed as feed. However, the current technology for preparing feed using chicken manure often has problems such as low protein content, high ammonia emissions, and low nitrogen utilization in chicken manure. Summary of the Invention

[0003] In view of the defects in the prior art, the purpose of the present invention is to provide a method for preparing an organic protein base material, which can significantly improve the nitrogen utilization rate of chicken manure, reduce ammonia emissions, and at the same time increase the protein content in feed prepared from chicken manure.

[0004] The purpose of the present invention is achieved through the following technical solutions:

[0005] The present invention provides a method for preparing an organic protein base material, comprising the following steps:

[0006] Performing spectral processing on chicken manure to obtain spectrally processed chicken manure;

[0007] Adjusting the carbon-nitrogen ratio of the spectrally treated chicken manure to (20-30):1 to obtain a compost base material;

[0008] The compost base material and the microbial agent are mixed and piled for fermentation to obtain an organic protein base material; the microbial agent comprises Candida utilis and Lactobacillus plantarum.

[0009] Preferably, the microbial agent is obtained by mixing Candida utilis seed solution and Lactobacillus plantarum seed solution in a volume ratio of 3:2; the bacterial activity of the Candida utilis seed solution is 1×10 8 ~5×10 8 CFU / mL; the bacterial activity of the Lactobacillus plantarum seed solution is 5×10 8 ~1×10 9 CFU / mL.

[0010] Preferably, the stacking fermentation includes two fermentation stages, namely the first fermentation stage and the second fermentation stage; the temperature of the first fermentation stage is 50-55°C; the first fermentation stage lasts from the start of fermentation to the third day of fermentation; the temperature of the second fermentation stage is 30-35°C; the second fermentation stage lasts from the fourth day of fermentation to the end of fermentation; the stacking fermentation lasts for 5-7 days.

[0011] Preferably, the pile is turned once every 16 to 32 hours during the fermentation process.

[0012] Preferably, the height of the pile during the stacking fermentation is 1 to 1.5 m, the width is 2 to 3 m, and the length is 2 to 4 m.

[0013] Preferably, when the compost base material and the microbial agent are mixed, the mass ratio of the microbial agent to the compost base material is (5-10):100.

[0014] Preferably, the raw materials used to adjust the carbon-nitrogen ratio of chicken manure for the spectral treatment include straw and / or molasses.

[0015] Preferably, the spectrum treatment method includes irradiation treatment using infrared and ultraviolet rays respectively;

[0016] When infrared and ultraviolet rays are used for irradiation treatment, the energy ratio of infrared treatment to ultraviolet treatment is 1:(1-4); the irradiation intensity of the infrared treatment and the ultraviolet treatment is 10-50 mW / cm 2 ; The spectral processing time is 20 to 40 minutes.

[0017] Preferably, the wavelength of the infrared ray is 760 μm to 1 mm; the wavelength of the ultraviolet ray is 250 to 280 nm.

[0018] The present invention provides an organic protein base material prepared by the preparation method described in the above technical solution.

[0019] Beneficial effects of the present invention:

[0020] The present invention provides a method for preparing an organic protein base, comprising the following steps: subjecting chicken manure to spectral treatment to obtain spectrally treated chicken manure; adjusting the carbon-nitrogen ratio of the spectrally treated chicken manure to (20-30):1 to obtain a compost base; and mixing the compost base with a microbial agent for fermentation to obtain an organic protein base; the microbial agent comprises Candida utilis and Lactobacillus plantarum. The preparation method provided by the present invention can significantly increase the protein content in chicken manure. The organic protein base prepared using the preparation method has high protein and essential amino acid content, contains probiotics, and meets nutritional and safety standards for feed, making it suitable for use as a livestock and poultry feed alternative to soybean meal. Furthermore, the preparation method provided by the present invention can significantly shorten the fermentation time of chicken manure and improve the efficiency of feed preparation from chicken manure. Experimental results demonstrate that the organic protein base prepared using the preparation method has a high protein content, with essential amino acids accounting for >30%. Furthermore, the organic protein base prepared by the present invention contains arsenic ≤10 mg / kg, lead ≤5 mg / kg, and no ascaris eggs or antibiotics detected. In summary, the preparation method provided by the present invention can significantly improve the processing efficiency of chicken manure, and at the same time increase the protein content in the feed prepared from chicken manure; the organic protein base prepared by the preparation method is suitable for use as feed for livestock and poultry farming. DETAILED DESCRIPTION

[0021] The present invention provides a method for preparing an organic protein base material, comprising the following steps:

[0022] Performing spectral processing on chicken manure to obtain spectrally processed chicken manure;

[0023] Adjusting the carbon-nitrogen ratio of the spectrally treated chicken manure to (20-30):1 to obtain a compost base material;

[0024] The compost base material and the microbial agent are mixed and piled for fermentation to obtain an organic protein base material; the microbial agent comprises Candida utilis and Lactobacillus plantarum.

[0025] The present invention performs spectral processing on chicken manure to obtain spectrally processed chicken manure. In the present invention, the chicken manure comprises fresh chicken manure. After obtaining the fresh chicken manure, the present invention preferably performs drying on the fresh chicken manure. The present invention does not particularly limit the drying method; conventional drying methods in the art may be employed. As an optional embodiment of the present invention, the chicken manure is preferably dried to a moisture content of 60%.

[0026] After obtaining chicken manure with a moisture content of 60%, the present invention performs spectral treatment on the chicken manure. The present invention does not specifically limit the method of the spectral treatment, and any conventional spectral treatment method in the field can be used. As an optional embodiment of the present invention, the spectral treatment method includes irradiating with infrared and ultraviolet rays respectively; when irradiating with infrared and ultraviolet rays, the energy ratio of infrared treatment and ultraviolet treatment can be 1: (1 to 4) or 1: 2; the irradiation intensity of the infrared treatment and the ultraviolet treatment can be 10 to 50 mW / cm 2 , can also be 10, 20, 30, 40 or 50 mW / cm 2 The time of the spectral treatment is 20 to 40 minutes, or 20, 25, 30, 35 or 40 minutes. As an optional embodiment of the present invention, the present invention can set the same irradiation intensity for infrared treatment and ultraviolet treatment, and regulate the energy ratio of infrared treatment and ultraviolet treatment by adjusting the irradiation time. In the embodiment of the present invention, by adjusting the irradiation intensity of infrared treatment to 30mW / cm 2 The irradiation time was 10 min and the irradiation intensity of ultraviolet treatment was 30 mW / cm 2 , the irradiation time is 20 minutes, and the energy ratio of infrared treatment and ultraviolet treatment is adjusted to 1:2. As an optional embodiment of the present invention, the wavelength of the infrared ray can be 760μm~1mm, or 760~800μm; the wavelength of the ultraviolet ray can be 250~280nm, or 265~270nm. As an optional embodiment of the present invention, the paving thickness of chicken manure during the spectral treatment process is ≤3cm, or 3, 2 or 1cm; the processing capacity of chicken manure during spectral treatment can be ≥5kg / h, or 5~10kg / h. In the embodiment of the present invention, chicken manure is treated by taking 2.5kg of chicken manure processed in 30 minutes as an example. After the spectral treatment is completed, spectrally treated chicken manure is obtained.

[0027] After obtaining the spectrally treated chicken manure, the present invention adjusts the carbon-nitrogen ratio of the spectrally treated chicken manure to (20-30):1 to obtain a compost base material. As an optional embodiment of the present invention, the raw materials for adjusting the carbon-nitrogen ratio of the spectrally treated chicken manure include straw and / or molasses; the straw can be corn straw. As an optional embodiment of the present invention, the straw can be fresh straw or dried straw. The present invention preferably crushes the spectrally treated chicken manure and straw and then mixes them to adjust the carbon-nitrogen ratio. The present invention does not specifically limit the method of crushing, and any conventional crushing method in the field can be used. As an optional embodiment of the present invention, the crushing can crush the spectrally treated chicken manure to a particle size of ≤2mm to obtain crushed chicken manure; the crushing can crush the straw to a particle size of ≤2mm to obtain crushed straw. After obtaining the crushed chicken manure and the crushed straw, the present invention mixes the crushed chicken manure and the crushed straw to adjust the carbon-nitrogen ratio to (20-30):1, or 20:1, 21:1, 22:1, 23:1, 24:1, 25:1, 26:1, 27:1, 28:1, 29:1 or 30:1.

[0028] After obtaining the compost base material, the present invention mixes the compost base material with a microbial agent and performs stacking fermentation to obtain an organic protein base material. Before the compost base material and the microbial agent are mixed, the moisture content of the compost base material is preferably adjusted to 40%. The present invention does not specifically limit the method for adjusting the moisture content, and any conventional adjustment method in the art can be used. As an optional embodiment of the present invention, the microbial agent is obtained by mixing a Candida utilis seed liquid and a Lactobacillus plantarum seed liquid in a volume ratio of 3:2; the bacterial activity of the Candida utilis seed liquid can be 1×10 8 ~5×10 8 CFU / mL, or 1×10 8 , 2×10 8 , 3×10 8 , 4×10 8 or 5×10 8 CFU / mL; the bacterial activity of the Lactobacillus plantarum seed solution can be 5×10 8 ~1×10 9 CFU / mL, or 5×10 8 , 6×10 8 , 7×10 8 , 8×10 8 , 9×10 8 CFU / mL or 10×10 8CFU / mL. As an optional embodiment of the present invention, when the compost base material and the microbial agent are mixed, the mass ratio of the microbial agent to the compost base material can be (5-10):100, or can be 5:100, 6:100, 7:100, 8:100, 9:100 or 10:100. After the compost base material and the microbial agent are mixed, the present invention performs a pile fermentation. As an optional embodiment of the present invention, the height of the pile during the stacking fermentation can be 1 to 1.5 m, or 1, 1.1, 1.2, 1.3, 1.4 or 1.5 m; the width can be 2 to 3 m, or 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9 or 3.0 m; the length can be 2 to 4 m, or 2, 2.1, 2.2, 2.3, 2.4, 2.5, 2.6, 2.7, 2.8, 2.9, 3, 3.1, 3.2, 3.3, 3.4, 3.5, 3.6, 3.7, 3.8, 3.9 or 4.0 m.

[0029] As an optional embodiment of the present invention, the stack fermentation comprises two stages: a first stage and a second stage. In the present invention, the temperature of the first stage can be 50-55°C, or alternatively 50, 51, 52, 53, 54, or 55°C; the first stage lasts from the start of fermentation to the third day of fermentation. The primary purpose of the first stage is to promote the decomposition of various complex organic substances in chicken manure. Within this temperature range, the enzyme activity of the microorganisms involved in the decomposition is high, enabling them to efficiently decompose macromolecules such as cellulose and chitin in the chicken manure, converting them into small molecules such as sugars and amino acids, providing the building blocks for subsequent fermentation and protein synthesis. In the present invention, the temperature of the second stage can be 30-35°C, or alternatively 30, 31, 32, 33, 34, or 35°C; the second stage lasts from the fourth day of fermentation to the end of fermentation. The primary purpose of the second stage is to promote protein synthesis. At this temperature, the metabolic activity of protein-producing microorganisms such as Candida utilis and Lactobacillus plantarum is more conducive to protein synthesis. Furthermore, lower temperatures can reduce excessive consumption of nutrients by microbial respiration, allowing more nutrients to be used for protein synthesis, thereby increasing the protein content in the final product. As an optional embodiment of the present invention, the stack fermentation period can be 5 to 7 days, or 5, 6, or 7 days. As an optional embodiment of the present invention, the stack fermentation process can be turned every 16 to 32 hours, or every 24 hours. When chicken manure is stacked and fermented using the preparation method described herein, the ammonia odor disappears after 5 to 7 days of stack fermentation, and a wine aroma is produced, indicating that the chicken manure fermentation is complete. The present invention utilizes a combination of Candida utilis and Lactobacillus plantarum to ferment chicken manure. Candida utilis enhances protein (SCP) synthesis, helping to increase the protein content of the final product during the chicken manure fermentation process. Lactobacillus plantarum lowers the pH of the fermentation environment, inhibits the growth of putrefactive bacteria, and acts as a deodorant. The combination of the two synergistically promotes the decomposition of chicken manure and increases its nutrient content. After stack fermentation is complete, the present invention can directly use the resulting fermentation product as an organic protein base.

[0030] As an optional embodiment of the present invention, the fermentation product can also be dried and granulated to obtain an organic protein base. As an optional embodiment of the present invention, the drying method includes low-temperature drying; the low-temperature drying temperature can be 50-80°C, or even 65°C; the drying is preferably performed to a moisture content of ≤12%. Low-temperature drying of the fermentation product in the present invention is primarily intended to preserve the activity of the probiotics. After drying, the dried product is granulated. The present invention does not specifically limit the granulation method; conventional granulation methods in the art can be used. As an optional embodiment of the present invention, the fermentation product can be mixed with bentonite for granulation; the mass ratio of the fermentation product to bentonite can be 100:(2-5), or 100:2, 100:3, 100:4, or 100:5. The present invention preferably obtains a granular organic protein base through granulation; the particle size of the granular organic protein base can be 3-5 mm, or 3, 4, or 5 mm.

[0031] The present invention processes chicken manure using the preparation method described in the above technical solution, significantly increasing the protein content in the manure, significantly shortening the time required to prepare feed from the manure, and improving the utilization efficiency of the manure. The preparation method provided by the present invention can reduce ammonia emissions from chicken manure by 60% to 80%, while simultaneously producing probiotic feed.

[0032] The present invention also provides an organic protein base material prepared by the preparation method described in the above technical solution. The organic protein base material provided by the present invention has a high protein content, a crude fiber mass percentage of ≤15%, a crude ash content of ≤15%, an essential amino acid content of ≥30%, an arsenic content of ≤10 mg / kg, a lead content of ≤5 mg / kg, no ascaris eggs and antibiotics detected, contains probiotics, meets the standards for livestock and poultry feed, and can be used as livestock and poultry feed. The organic protein base material provided by the present invention can replace soybean meal and has a cost that is more than 30% lower than soybean meal.

[0033] In order to further illustrate the present invention, the technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0034] The sources of the bacterial agents and the cultivation methods of the bacterial agents used in the following technical solutions:

[0035] Candida utilis was purchased from Shanghai Kimi Biotechnology Co., Ltd., and the product name is: Candida utilis.

[0036] Lactobacillus plantarum was purchased from Peptide Biotechnology (Xi'an) Co., Ltd., and the product name is: Lactobacillus plantarum 10 billion cfu / g.

[0037] Preparation method of Candida utilis seed solution: inoculate Candida utilis into malt extract medium (pH 4.5-5.5) for cultivation at a temperature of 25-28°C in a shaker at a speed of 150-200 r / min for 24-36 hours. The obtained bacterial activity is 1×10 8 ~5×10 8 CFU / mL of Candida utilis seed solution.

[0038] Preparation method of Lactobacillus plantarum seed solution: Lactobacillus plantarum was inoculated into MRS medium (pH value was 6.0-6.5) for cultivation at a temperature of 30-37°C and cultured under an anaerobic environment for 48-72 hours to obtain a bacterial activity of 5×10 8 ~1×10 9 CFU / mL of Lactobacillus plantarum seed solution.

[0039] The Candida utilis seed liquid and the Lactobacillus plantarum seed liquid are mixed in a volume ratio of 3:2 to obtain a bacterial agent for chicken manure fermentation, which can also be called a microbial bacterial agent.

[0040] Example 1

[0041] An organic protein base material, the preparation method steps are as follows:

[0042] The moisture content of chicken manure was adjusted to 60%. The chicken manure with a moisture content of 60% was spread in a spectrum processing device with a thickness of 3 cm and subjected to spectrum processing. The infrared irradiation time during the spectrum processing was 10 minutes. After the infrared irradiation treatment was completed, ultraviolet irradiation treatment was performed. The ultraviolet irradiation time was 20 minutes. The wavelength of the infrared ray was 760 μm, the wavelength of the ultraviolet ray was 265 nm, and the irradiation intensity of the infrared ray was 30 mW / cm 2 , the UV radiation intensity is 30mW / cm 2 The total spectral irradiation treatment time is 30 minutes. After the spectral treatment is completed, the spectral treated chicken manure is obtained.

[0043] After obtaining the spectrally treated chicken manure, the resulting chicken manure is pulverized to a particle size of ≤2 mm to obtain chicken manure with a particle size of ≤2 mm. The chicken manure with a particle size of ≤2 mm is then mixed with corn straw powder (the particle size of the corn straw powder is ≤2 mm). The amount of corn straw powder is such that the carbon-nitrogen ratio in the mixture of chicken manure and corn straw powder is 25:1, thereby obtaining a compost base.

[0044] The inoculant used for chicken manure fermentation was added to the compost base at a mass percentage of 8% (w / w), mixed evenly, and the moisture content of the compost base was adjusted to 40%. After inoculation, the inoculated compost base was piled for fermentation; the pile height during the fermentation was 1m, the width was 2m, and the length was 2m.

[0045] During the pile fermentation process, the temperature was maintained at 50-55°C from the beginning to the third day of fermentation, and at 30-35°C from the fourth day to the end of fermentation. The pile fermentation lasted for 7 days. The pile was turned every 24 hours to ensure uniform aeration. After the completion of the pile fermentation, the chicken manure fermentation product was obtained.

[0046] The chicken manure fermentation product is low-temperature dried at 65°C to a moisture content of ≤12% to retain the activity of the probiotics, thereby obtaining a dried chicken manure fermentation product. 2% to 5% (w / w) bentonite is added to the dried chicken manure fermentation product to prepare 3 to 5 mm particles to obtain an organic protein base.

[0047] Comparative Example 1

[0048] A method for preparing an organic protein base material is the same as that in Example 1, except that only Candida utilis is used to ferment chicken manure, and the bacterial activity is 1×10 8 ~5×10 8 CFU / mL of Candida utilis seed liquid was used as a bacterial agent for chicken manure fermentation and was added to the compost base material at a mass percentage of 8% (w / w).

[0049] Comparative Example 2

[0050] A method for preparing an organic protein base material is the same as that in Example 1, except that only Lactobacillus plantarum is used to ferment chicken manure, and the bacterial activity is 5×10 8 ~1×10 9 CFU / mL of Lactobacillus plantarum seed liquid was added to the compost base material at a mass percentage of 8% (w / w) as a bacterial agent for chicken manure fermentation.

[0051] Application Example 1

[0052] The nutritional indicators and safety indicators of the organic protein base prepared in Example 1 were tested, wherein the crude protein content was determined by the Kjeldahl method (GB 5009.5); the essential amino acid ratio was determined by an amino acid analyzer (GB 5009.124); and the probiotic activity was determined by the plate count method (GB 4789.35).

[0053] The test results are shown in Table 1.

[0054] The nutritional indicators of the organic protein base prepared in Example 1 are as follows: crude protein content is 38.9%, crude fiber content is 14.1%, crude ash content is 8.1%, and essential amino acids account for 31.1%.

[0055] The organic protein base prepared in Example 1 contained heavy metals such as arsenic ≤ 10 mg / kg and lead ≤ 5 mg / kg. No ascaris eggs or antibiotics were detected.

[0056] The nutritional indicators and safety indicators of the organic protein base prepared in Example 1 were tested, and the results are shown in Table 1.

[0057] Table 1 Nutritional and safety index test results of the organic protein base prepared in Example 1

[0058]

[0059]

[0060] Note: “——” in the table means that the relevant indicators were not detected.

[0061] Application Example 2

[0062] The nutritional indicators of the organic protein bases prepared in Example 1 and Comparative Examples 1-2 were tested, and the test results are shown in Table 2.

[0063] Table 2 Nutritional index test results of the organic protein base prepared in Example 1 and Comparative Examples 1-2

[0064] parameter Example 1 Comparative Example 1 Comparative Example 2 Protein content 38.9% 28.5% 22.3% Essential amino acids ratio 31.1% 22.7% 18.5% Live probiotics <![CDATA[5.5×10 8 CFU / g]]> <![CDATA[2.1×10 8 CFU / g]]> <![CDATA[3.2×10 8 CFU / g]]>

[0065] The crude protein content of Example 1 is significantly higher than that of Comparative Examples 1 and 2, indicating that the combination of Candida utilis and Lactobacillus plantarum can synergistically promote protein synthesis. Although single Candida utilis can decompose organic matter, it lacks acidic environment support (Lactobacillus plantarum can lower the pH value to inhibit miscellaneous bacteria), resulting in insufficient protein synthesis efficiency; single Lactobacillus plantarum has limited protein enhancement effect due to the lack of efficient nitrogen-fixing bacteria. The difference in the proportion of essential amino acids further illustrates that the mixed bacterial agent converts chicken manure nitrogen source into high-quality protein more efficiently through the synergistic effect of "Candida utilis synthesizing protein and Lactobacillus plantarum optimizing the metabolic environment". The probiotic activity of Example 1 is the highest because the two bacteria form a symbiotic system during fermentation: Lactobacillus plantarum inhibits the growth of miscellaneous bacteria by producing acid, providing a suitable breeding environment for Candida utilis; the metabolites of Candida utilis (such as small molecule organic matter) can promote the proliferation of Lactobacillus plantarum. The lack of this synergy in a single bacterial agent results in a low total amount of probiotics. Experimental data show that a single microbial agent (Candida utilis or Lactobacillus plantarum) has limited effect in increasing the protein content and probiotic activity of chicken manure, while the combination of the two can produce significant synergistic effects through functional complementarity.

[0066] Although the above embodiment provides a detailed description of the present invention, it is only a part of the embodiments of the present invention, not all of the embodiments. People can also obtain other embodiments based on this embodiment without creativity, and these embodiments all fall within the scope of protection of the present invention.

Claims

1. A method for preparing an organic protein base, characterized in that: The following steps are involved: Performing spectral processing on chicken manure to obtain spectrally processed chicken manure; Adjusting the carbon-nitrogen ratio of the spectrally treated chicken manure to (20-30):1 to obtain a compost base material; The compost base material and the microbial agent are mixed and piled for fermentation to obtain an organic protein base material; the microbial agent comprises Candida utilis and Lactobacillus plantarum.

2. The preparation method according to claim 1, characterized in that The microbial agent is obtained by mixing Candida utilis seed liquid and Lactobacillus plantarum seed liquid in a volume ratio of 3:2; the bacterial activity of the Candida utilis seed liquid is 1×10 8 ~5×10 8 CFU / mL; the bacterial activity of the Lactobacillus plantarum seed solution is 5×10 8 ~1×10 9 CFU / mL.

3. The preparation method according to claim 1, characterized in that: The stacking fermentation includes two fermentation stages, namely the first fermentation stage and the second fermentation stage; the temperature of the first fermentation stage is 50-55° C.; the first fermentation stage lasts from the start of fermentation to the third day of fermentation; the temperature of the second fermentation stage is 30-35° C.; the second fermentation stage lasts from the fourth day of fermentation to the end of fermentation; and the stacking fermentation lasts for 5-7 days.

4. The preparation method according to claim 1, characterized in that During the fermentation process, the pile should be turned over once every 16 to 32 hours.

5. The preparation method according to claim 1, characterized in that: During the stacking fermentation, the material pile has a height of 1 to 1.5 m, a width of 2 to 3 m, and a length of 2 to 4 m.

6. The preparation method according to claim 1, characterized in that: When the compost base material and the microbial agent are mixed, the mass ratio of the microbial agent to the compost base material is (5-10):

100.

7. The preparation method according to claim 1, characterized in that: The raw materials used to adjust the carbon-nitrogen ratio of the chicken manure subjected to the spectrum treatment include straw and / or molasses.

8. The preparation method according to claim 1, characterized in that: The spectrum treatment method includes irradiation treatment using infrared and ultraviolet rays respectively; When infrared and ultraviolet rays are used for irradiation treatment, the energy ratio of infrared treatment to ultraviolet treatment is 1:(1-4); the irradiation intensity of the infrared treatment and the ultraviolet treatment is 10-50 mW / cm 2 ; The spectral processing time is 20 to 40 minutes.

9. The preparation method according to claim 8, characterized in that: The wavelength of the infrared ray is 760 μm to 1 mm; the wavelength of the ultraviolet ray is 250 to 280 nm.

10. The organic protein base prepared by the preparation method according to any one of claims 1 to 9.

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