Method for promoting transportation of calcium element to overground part of plant

Abstract

According to the method for promoting the calcium element to be transported to the overground part of the plant, the formula of a related nutrient solution is reasonably adjusted, particularly, nickel element with specific concentration is introduced, so that the nickel element and other nutrient elements play a role together, and the calcium element is effectively promoted to be transported to the overground part of the plant; therefore, further growth and development of the plants are effectively promoted, and the yield and quality of the plants are improved.

Description

Technical Field

[0001] The invention belongs to the technical field of agricultural planting, and in particular relates to a method for promoting the transport of calcium to the aboveground parts of plants. Background Art

[0002] Calcium is one of the most important elements in plant nutritional physiology research. It is not only a macronutrient necessary for plant growth and development, but more importantly, it participates in the regulation of plant growth and development as a second messenger of extracellular signals and intracellular physiological and biochemical reactions.

[0003] Calcium is an element that is difficult for plants to absorb and, once absorbed, is not easily mobilized. Large amounts of calcium are found in leaves, but very little in fruit. This means that calcium deficiency in the aerial parts of plants, especially in the apical buds and fruit, is common, even in soils with sufficient calcium.

[0004] Calcium deficiency in the aboveground parts of plants generally presents the following symptoms:

[0005] 1. Calcium-deficient crops often experience curled and yellowing leaves. This affects the leaf's normal morphology and function, causing it to become lighter in color. In severe cases, necrotic spots may appear on the leaves, hindering photosynthesis and reducing the crop's ability to produce organic matter, ultimately impacting overall growth and yield.

[0006] 2. At the stem tip, calcium deficiency will cause the apical bud to stagnate. In severe cases, the plant will be unable to grow upward normally, resulting in "top withering" or even death.

[0007] 3. For fruit crops, calcium deficiency can lead to a variety of problems. For example, apples are prone to bitter pit, which causes sunken spots on the surface of the fruit and necrosis of the flesh. Tomatoes are also susceptible to navel rot, which causes water-soaked spots on the navel of the fruit that gradually expand and turn dark brown, seriously affecting the appearance and edible value of the fruit. Calcium deficiency also reduces the firmness of the fruit, making it less resistant to storage and transportation and more susceptible to rot and deterioration.

[0008] In view of this, how to improve the mobility of calcium in plants is a major issue in agriculture and plant biology. Summary of the Invention

[0009] To address the problems and deficiencies in the prior art, the present invention provides a method for promoting the transport of calcium to the aboveground parts of plants. This method rationally adjusts the formula of the relevant nutrient solution, particularly by introducing a specific concentration of nickel, so that it works together with other nutrient elements to effectively promote the transport of calcium to the aboveground parts of plants, thereby effectively promoting the further growth and development of the plants and improving the yield and quality of the plants.

[0010] The present invention provides a method for promoting the transport of calcium to the aerial part of a plant. The specific steps are as follows: selecting plant germinated seeds that have grown roots with a root length of 2 to 6 cm; during the growth process of the plant germinated seeds, using a basic nutrient solution A comprising the following components: Ca(NO3)2·4H2O 2.5mM, KH2PO4 1mM, KCl 50mM, MgSO4·7H2O 2mM, K2SO4 2mM, FeSO4·7H2O 0.1mM, EDTA·Na2 0.1mM, MnCl2 2μM, ZnSO4·7H2O 4μM, CuSO4·5H2O 0.5μM, (NH4)6Mo7O 24 ·4H2O 1μM, H3BO3 25μM, NiCl2 2~20μM; the basic nutrient solution B includes the following ingredients: Ca(NO3)2·4H2O 2.5mM, KH2PO4 1mM, KCl 50mM, MgSO4·7H2O 2mM, K2SO4 2mM, FeSO4·7H2O0.1mM, EDTA·Na2 0.1mM, MnCl2 2μM, ZnSO4·7H2O 4μM, CuSO4·5H2O 0.5μM, (NH4)6Mo7O 24 4H2O 1μM, H3BO3 25μM, NiCl2 2-20μM; Nutrients were applied as follows: (1) for 1-3 days, a first nutrient solution was used, in which the concentration of all components in the first nutrient solution was 20-30% of that in the basic nutrient solution A; (2) for 4-10 days, a second nutrient solution was used, in which the concentration of all components in the second nutrient solution was 20-30% of that in the basic nutrient solution B.

[0011] Soilless cultivation technology is a new cultivation technology in modern agriculture. It has changed the traditional cultivation model, got rid of the restrictions of land, and planted plants directly in nutrient solution, or planted them in substrate and irrigated with nutrient solution. Soilless cultivation has a high-quality rhizosphere environment, so it can effectively prevent soil diseases and pests caused by continuous cropping and physiological disorders caused by soil salt accumulation. At the same time, soilless cultivation can meet all the plant's needs for nutrients, water, gas and other conditions. The substrate and nutrient solution can also be used multiple times, and the cultivation site is not restricted. Therefore, it has many characteristics such as easy management, high yield and high quality. Due to the above advantages, soilless cultivation has broad development prospects in the agricultural field.

[0012] Nutrient solution provides all the nutrients needed for plant growth. Nutrient requirements vary from crop to crop, so the most appropriate nutrient solution formula must be selected based on the crop to achieve the best results. The biggest difference between nutrient solutions is the content of each nutrient element, so the nutrient solution formula and concentration are the determining factors for plant growth. Nutrient solution management is a very important step in soilless cultivation, especially under conditions with a low degree of automation. Nutrient solution management is even more important because plants are sensitive to the content of various nutrients in the nutrient solution. Inappropriate ratios are more likely to cause various physiological diseases, which directly affect plant growth.

[0013] For example, nitrogen, phosphorus, and potassium are macronutrients essential for plant growth. Nitrogen is a component of many important compounds in plants, such as proteins, nucleic acids, and chlorophyll. It promotes luxuriant growth and improves crop yield and quality. Nitrogen deficiency slows plant growth and causes leaves to turn yellow. Phosphorus participates in important physiological processes in plants, such as energy metabolism and photosynthesis. It promotes root development, enhances stress resistance, and promotes flowering and fruiting. Phosphorus deficiency results in stunted growth, dark green leaves, and delayed flowering and fruiting. Calcium, magnesium, and sulfur are other macronutrients essential for plant growth. Calcium, a key component of calcium pectate in plant cell walls, stabilizes cell wall structure, enhances stress resistance, and strengthens disease resistance. It also participates in signal transduction in plant cells and regulates plant growth and development. Magnesium, a component of chlorophyll, is crucial for photosynthesis. It also participates in enzymatic reactions in plants, promoting phosphorus absorption and utilization. Magnesium deficiency causes leaves to lose their green color and turn yellow. Sulfur is a component of many sulfur-containing amino acids and proteins in plants and plays a role in plant metabolism and stress resistance. Sulfur deficiency causes yellowing of plant leaves and stunted growth. Iron, zinc, manganese, copper, boron, and molybdenum are trace elements. Although plants require them in small quantities, these trace elements play an indispensable role in plant growth and development. They participate in enzymatic reactions and electron transport processes within plants, influencing physiological functions such as photosynthesis, respiration, and hormone synthesis.

[0014] Moreover, during the growth of plants, the formula of the nutrient solution will also have a significant impact on the absorption of various nutrients and their transport to the aboveground parts of the plants.

[0015] For example, the concentration and ratio of various ions in the nutrient solution will affect the absorption of nutrients by plants. When the concentration of a certain ion in the nutrient solution is too high, it may inhibit the plant's absorption of other ions. This phenomenon is called ion antagonism. For example, ammonium nitrogen (NH4 + ) concentration is too high will inhibit the plant's absorption of potassium (K + ), calcium (Ca 2+ ), magnesium (Mg 2+ ) plasma absorption, because NH4 +These cations compete for adsorption sites on the surface of plant roots. Conversely, a proper ion balance can promote plant absorption of various nutrients. For example, an appropriate amount of phosphorus (P) can promote plant absorption of zinc (Zn) because P participates in energy metabolism and material transport within plants, facilitating the transport and absorption of Zn within the plant.

[0016] For example, nutrients in the nutrient solution need to enter the cells via carrier proteins and channel proteins on the cell membranes of plant roots and then be transported to the aboveground parts through the xylem and phloem. Certain ingredients in the nutrient solution formula may affect the synthesis, activity, or expression of these carrier and channel proteins, thereby affecting the transport of nutrients. For example, an appropriate amount of boron (B) can promote the expression of aquaporins on the plasma membrane of plant roots, thereby improving the plant's absorption and transport efficiency of water and some small molecule nutrients. However, a boron deficiency can hinder the synthesis of pectin in the cell wall, affecting cell elongation and division, and further impairing the transport of nutrients to the aboveground parts. Furthermore, some ingredients in the nutrient solution formula can affect the synthesis, transport, and distribution of hormones in the plant, indirectly affecting the transport of nutrients to the aboveground parts. For example, auxin and cytokinin play important regulatory roles in plant growth and development. An appropriate amount of nitrogen can promote the synthesis of auxin in the plant, thereby promoting root growth and nutrient absorption, while also facilitating the transport of nutrients to the aboveground parts.

[0017] Therefore, the formulation of the nutrient solution is particularly important for the plant growth process, especially for the absorption of various nutrients during plant growth and the transport of nutrients to the aboveground parts of the plant, which in turn affects the growth and development of the plant, and thus affects the yield and quality of the plant. In particular, calcium is an essential medium-sized element in the plant growth process and is crucial to the growth and development of the plant. However, calcium deficiency in the aboveground parts of plants, especially in the apical buds and fruits, is very common, even in soils with sufficient calcium content. The main reason for plant calcium deficiency is the poor mobility of calcium. How to improve the mobility of calcium in plants is a major problem in agriculture and plant biology.

[0018] In view of this, the present invention, in a long-term experimental process, by reasonably adjusting the formula of the nutrient solution, and by adjusting the nutrient solution concentration applied at different growth stages, a more suitable culture method is provided, which effectively improves the absorption of each nutrient element and the plant aboveground transport of each nutrient element, especially significantly promotes calcium element to be transported to the plant ground, therefore effectively promotes the growth and development of plants, improves the yield and quality of plants. Specifically, under the specific nutrient solution formula and specific nutrient ingredient application method provided by the present invention, certain synergistic effects can be produced between each nutrient element of a specific concentration, particularly under specific nickel element concentration, further promotes each nutrient element and the plant aboveground transport of each nutrient element, especially significantly promotes calcium element to be transported to the plant ground, and then makes final plant obtain better growth and development, makes plant yield and quality all obtain significantly improved. Simultaneously, applying nutrient solution of different concentrations at different times can both ensure the demand of plant to nutrient element and avoid nutrient element excess, therefore can more effectively promote the growth and development of plants, improve plant yield and quality. It should be noted that excessive nickel concentrations, particularly those exceeding 20 μM, can cause plant toxicity, including decreased chlorophyll, inhibited pea growth, and reduced biomass. Overall, a nickel concentration of 2 to 10 μM is generally beneficial for overall plant growth and development.

[0019] Preferably, the plant germinated seeds are derived from beans and cruciferous vegetables.

[0020] Preferably, the germinating plant seeds include peas. Peas are important crops with high demand in both domestic and international markets. Therefore, high yield and quality of peas are crucial for agricultural development. The cultivation method provided by the present invention utilizes a specific nutrient solution formulation and application methods at specific concentrations at different stages to enhance the absorption of various nutrients, particularly calcium, during pea growth. The high concentration of nickel, in particular, further enhances calcium transport through the peas' growth. Therefore, the method is more suitable for pea growth and development, thereby enhancing pea yield and quality.

[0021] Preferably, plant germinated seeds that have grown roots and have a root length of 3 to 4 cm are selected. Selecting plant germinated seeds with a root length of 3 to 4 cm is conducive to the synchronous growth of subsequent plant germinated seeds, that is, it is conducive to the balanced absorption of nutrient solution by each plant germinated seed, avoiding the situation where, for example, some plant germinated seeds have too short roots and the nutrient solution concentration is relatively excessive, which affects the normal growth and development of these plant germinated seeds, thereby causing a decrease in the yield or quality of overall plant growth and development.

[0022] Preferably, during the growth of plant germination seeds, the temperature is controlled at 20-27°C and the humidity is controlled at 70-80%. Suitable temperature and humidity are more conducive to the further growth and development of plant germination seeds, and are also more conducive to the absorption of various nutrients and the above-ground transportation of calcium, thereby improving plant yield and quality.

[0023] Preferably, during the growth of plant germination seeds, light treatment is also applied: 16 h light / 8 h dark cycle; light intensity is 60-250 μmol·m -2 ·s -1 Furthermore, proper light can promote plant photosynthesis, while promoting the absorption of various nutrients and the above-ground transportation of calcium, thereby improving plant yield and quality.

[0024] Preferably, the concentration of NiCl2 in the basal nutrient solution B is 2 to 5 μM. At this nickel concentration, not only is the aboveground transport of calcium more favorable, but it also facilitates the interaction of the various nutrients in the overall nutrient solution, further promoting plant growth and development, and improving plant yield and quality.

[0025] Preferably, the concentration of NiCl2 in the basal nutrient solution B is 5 μM.

[0026] Preferably, the pH of the first nutrient solution is 5-6; the pH of the second nutrient solution is 5-6. The pH value of the nutrient solution will affect the existence form of nutrients and the physiological characteristics of plant roots, thereby affecting the absorption of nutrients. Under acidic conditions, some cations such as iron (Fe 3+ ), aluminum (Al 3+ ) increases the solubility of trace elements such as iron (Fe), manganese (Mn), and zinc (Zn), which can lead to excessive absorption of these elements by plants, resulting in toxic effects. Under alkaline conditions, some trace elements such as iron (Fe), manganese (Mn), and zinc (Zn) form poorly soluble hydroxides or carbonates, reducing their effectiveness and making it difficult for plants to absorb them. Therefore, at this pH, plant absorption of nutrients is effectively promoted, while also effectively facilitating the aboveground transport of calcium, further enhancing plant growth and development.

[0027] Preferably, the cultivation method for obtaining plant germinated seeds includes at least one of hydroponics and soil cultivation.

[0028] Preferably, obtaining plant germinated seeds includes the following culture steps: step 1, soaking the plant seeds in a disinfectant and washing them with deionized water until there is no obvious pungent odor; step 2, soaking the sterilized plant seeds in a dark environment at 20-26°C for 8-15 hours, then transferring them to a seedling tray and culturing them in a dark environment at 20-26°C for 2-4 days until the roots grow to 3-4 cm.

[0029] Preferably, in step 1, the plant seeds are soaked in a 7.5% sodium hypochlorite (AR) solution for 30 minutes. During the soaking period, shriveled, thin, damaged, or dull seeds are removed using plastic tweezers. The seeds are then repeatedly rinsed with ultrapure water until no noticeable pungent odor remains. "7.5%" refers to the mass fraction.

[0030] Preferably, in step 2, the sterilized plant seeds are placed in a dark environment at 24°C and soaked for 12 hours. After 12 hours, they are transferred to a seedling tray, pure water is added to the seedling tray until the liquid surface contacts the plant seeds, and gauze is soaked and covered with the plant seeds; the plant seeds are cultured in a dark environment at 24°C for 3 days, during which time moldy and rotten seeds are promptly removed until the roots grow to about 3 to 4 cm.

[0031] In summary, the cultivation method provided by the present invention effectively promotes the absorption of various nutrient elements in the plant and the upward transport of various nutrient elements, especially calcium, by rationally adjusting the concentrations of various nutrient elements in the nutrient solution and applying different concentrations of nutrient solution at different plant growth stages, particularly by adding a particular concentration of nickel. This effectively promotes plant growth and development, and improves plant yield and quality. In particular, the cultivation method provided by the present invention has a more pronounced effect on promoting the upward transport of calcium and growth and development in peas. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 The calcium content in the roots and aerial parts of peas at different nickel concentrations in Example 1 is shown.

[0033] Figure 2 The figures show the effects of nickel treatments at different nickel concentrations on the biomass (fresh weight and dry weight) of pea roots and aboveground parts in Example 1; A and B correspond to the wet weight of the aboveground parts and roots of peas, respectively, and C and D correspond to the dry weight of the aboveground parts and roots of peas, respectively. DETAILED DESCRIPTION

[0034] In order to enable those skilled in the art to better understand the solutions of the present invention, the technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments.

[0035] Example 1

[0036] 1. Test materials:

[0037] 'Zhongwan No. 6' was selected as the test material.

[0038] 2. Plant cultivation method:

[0039] Step 1: Soak pea seeds in a 7.5% sodium hypochlorite (AR) solution for 30 minutes, using plastic tweezers to remove pea seeds that are shriveled, thin, have damaged skin, or are dull in color, and then repeatedly rinse with ultrapure water until no obvious pungent odor remains.

[0040] Step 2: After sterilizing the pea seeds, soak them in a dark environment at 24° C. for 12 hours. After 12 hours, transfer them to a special seedling tray for sprouts, add pure water to the seedling tray until the liquid surface contacts the seeds, and cover the pea seeds with soaked gauze; incubate the pea seeds in a dark environment at 24° C. for 3 days, during which time moldy and rotten pea seeds are promptly removed until the pea roots grow to about 3-4 cm;

[0041] Step 3: The basic nutrient solution A includes the following ingredients: Ca(NO3)2·4H2O 2.5mM, KH2PO4 1mM, KCl50mM, MgSO4·7H2O 2mM, K2SO4 2mM, FeSO4·7H2O 0.1mM, EDTA·Na20.1mM, MnCl2 2μM, ZnSO4·7H2O 4μM, CuSO4·5H2O 0.5μM, (NH4)6Mo7O 24 4H2O 1μM, H3BO3 25μM;

[0042] The basic nutrient solution B includes the following ingredients: Ca(NO3)2·4H2O 2.5mM, KH2PO4 1mM, KCl50mM, MgSO4·7H2O 2mM, K2SO4 2mM, FeSO4·7H2O 0.1mM, EDTA·Na2 0.1mM, MnCl2 2μM, ZnSO4·7H2O 4μM, CuSO4·5H2O 0.5μM, (NH4)6Mo7O 24 4H2O 1μM, H3BO3 25μM, NiCl2 2-10μM; select peas with uniform growth (pea roots in step 2 are about 3-4cm long) and apply the nutrients as follows:

[0043] (1) For 1-3 days, the first nutrient solution (pH 5.5) was used, and the concentration of all components in the first nutrient solution was 25% of that in the basic nutrient solution A;

[0044] (2) From 4 to 10 days, a second nutrient solution (pH 5.5) was used, and the concentration of all components in the second nutrient solution was 25% of that in the basic nutrient solution B.

[0045] During the growth of peas, the temperature was controlled at 20°C, the humidity was controlled at 70%, and the light intensity was controlled at 200 μmol·m -2 ·s -1The light and dark periods were 16 h and 8 h, respectively, with a cycle of light and dark periods.

[0046] Among them, in step 3 (2), the basic nutrient solution B used was set with different nickel element content groups, specifically 0μM NiCl2 (control), 2μM NiCl2, 5μM NiCl2, 10μM NiCl2, 20μM NiCl2, 40μM NiCl2, and 60μM NiCl2.

[0047] After a certain number of days of cultivation according to (1)-(2) in step 3 above, the calcium content of different parts of the peas is tested, mainly the calcium content of the roots and the aboveground parts of the peas. The sampling area of ​​the aboveground parts of the peas is the stems and leaves, and the sampling area of ​​the underground parts is the entire root system. The specific method for testing the calcium content is as follows:

[0048] After collecting the plant samples, rinse them with tap water first, then rinse them three times with deionized water to remove residual ions on the surface that may affect the test results. The different parts of the plant were divided and fixed at 80-105℃ for 30 minutes, and then dried at 60-80℃ to constant weight for later use. Weigh 0.1g of dry sample (take the root and above-ground parts of peas respectively), grind them, put them into a clean 10mL digestion tube, add 2mL of concentrated H2SO4 and soak for 12h, then digest them in a digestion furnace at 150℃ until the sample tissue is completely digested. Slowly add H2O2 until the sample becomes colorless and transparent. After natural cooling, transfer the liquid to a 10mL centrifuge tube, filter it to the constant volume, and determine the calcium content using ICP-OES. Finally, calculate the calcium content in different parts of the peas.

[0049] The test results are as follows Figure 1 As shown, from Figure 1 It can be seen that adding 2-20 μM nickel nutrient elements during the cultivation process can significantly promote the calcium content in the aboveground part, which increased by 88.0% (corresponding to 2 μM nickel), 115.4% (corresponding to 5 μM nickel), 181.2% (corresponding to 10 μM nickel), and 210.67% (corresponding to 20 μM nickel) compared with the control group.

[0050] At the same time, in this example, in order to further verify the effect of the specific formula of the nutrient solution provided in this example and the application of different concentrations of nutrient solution at different stages on the overall growth and development of peas, different concentrations of nickel content groups were used in this example. After 7 days of cultivation (nickel treatment time, excluding the early adaptation time), the fresh weight and dry weight of the pea plants were measured and recorded. The results showed that 2μM and 5μM nickel treatments helped to increase the biomass of pea plants. Figure 2The fresh and dry weights of pea roots and aerial parts increased significantly after treatment with 2-5 μM nickel. This demonstrates that the specific culture method of the present invention can effectively promote the growth and development of pea plants and increase their yield. It should be noted that excessive amounts of nickel, as a heavy metal, are detrimental to plant growth and human health. Therefore, a lower concentration (2-5 μM) is more beneficial to both the growth and development of plants such as peas and human health.

[0051] Comparative Example 1

[0052] The difference between this comparative example and Example 1 is that in step 3 (2), the concentration of the second nutrient solution applied is 10% of the basic nutrient solution B, and the concentration of NiCl2 in the basic nutrient solution B is 5 μM. The rest of the culture steps are consistent with those in Example 1.

[0053] Calcium content was tested in the root and aerial parts of peas, respectively. The specific testing method was as described in Example 1. The results showed that the calcium content in the root and aerial parts of peas was lower than that in the 5 μM NiCl2 group in Example 1. This indicates that when the concentration of the second nutrient solution is too low, it will affect the transport of calcium to the aerial parts, thereby reducing the accumulation of this element in the aerial parts.

[0054] Referring to Example 1, the fresh and dry weights of pea plants were measured and recorded. The results showed that compared to the 5 μM NiCl2 group in Example 1, the wet and dry weights of the roots and aerial parts of the peas were lower. This suggests that lowering the concentration of the second nutrient solution slowed pea growth, which is detrimental to pea growth and development. However, during the cultivation process, we found that plants treated with nickel in low-concentration nutrient solutions grew better than those not treated with nickel. This demonstrates that trace amounts of nickel can promote pea growth.

[0055] Comparative Example 2

[0056] The difference between this comparative example and Example 1 is that in step 3 (2), the concentration of the second nutrient solution applied is 40% of the basic nutrient solution B, and the concentration of NiCl2 in the basic nutrient solution B is 5 μM. The rest of the culture steps are consistent with those in Example 1.

[0057] The calcium content of the root and aerial parts of peas was tested respectively. The specific testing method was referred to Example 1. The results showed that although the calcium content of the aerial parts of peas increased, the pea plants treated with 20 μM nickel showed poisoning.

[0058] Referring to Example 1, the fresh weight and dry weight of the pea plants were measured and recorded. Because the NiCl2 concentration in basal nutrient solution B was too high at 20 μM, causing toxicity in the pea plants, the fresh weight and dry weight of the pea plants were somewhat lower than those in the 5 μM NiCl2 group in Example 1.

[0059] Comparative Example 3

[0060] The difference between this comparative example and Example 1 is that in steps (1) and (2) of step 3, the second nutrient solution is applied, and the concentration of NiCl2 in the basic nutrient solution B is 5 μM. The rest of the culture steps are consistent with those in Example 1.

[0061] Calcium content was measured in the root and aerial parts of peas, using the same testing method as in Example 1. The results showed that calcium content decreased in both the root and aerial parts. This suggests that using the second nutrient solution for 1-10 days, without the initial acclimatization phase using the first nutrient solution, can affect calcium accumulation in the aerial parts of the peas.

[0062] Referring to Example 1, the fresh weight and dry weight of the pea plants were measured and recorded. Because the peas were not initially acclimated to the first nutrient solution, their growth and development were affected. Therefore, the fresh weight and dry weight of the pea plants were somewhat lower than those of the 5 μM NiCl2 group in Example 1. This demonstrates that acclimation to the first nutrient solution (basal nutrient solution A) is crucial for the later growth of peas.

[0063] Comparative Example 4

[0064] The difference between this comparative example and Example 1 is that both the basic nutrient solution A and the basic nutrient solution B do not contain (NH4)6Mo7O 24 4H2O, that is, in step 3 (1) (2), there is no (NH4)6Mo7O 24 4H2O, and the concentration of NiCl2 in the basic nutrient solution B is 5μM. The rest of the culture steps are the same as those in Example 1.

[0065] The calcium content of the root and aboveground parts of peas was tested respectively. The specific testing method was referred to Example 1. The results showed that the calcium content of the root and aboveground parts of peas was reduced. This indicates that the lack of (NH4)6Mo7O 24 4H2O will affect the transport of calcium to the aerial parts of peas.

[0066] With reference to Example 1, the fresh weight and dry weight of pea plants were measured and recorded. Nitrogen and molybdenum are essential nutrients for plants. 244H2O inhibited pea biomass accumulation, so the fresh weight and dry weight of pea plants were somewhat reduced compared to the 5 μM NiCl2 group in Example 1.

[0067] Comparative Example 5

[0068] The difference between this comparative example and Example 1 is that both the basic nutrient solution A and the basic nutrient solution B do not contain (NH4)6Mo7O 24 4H2O and H3BO3, that is, in step 3 (1) and (2), neither contains (NH4)6Mo7O 24 4H2O and H3BO3, and the concentration of NiCl2 in the basic nutrient solution B is 20μM. The rest of the culture steps are the same as in Example 1.

[0069] The calcium content of the root and aboveground parts of peas was tested respectively. The specific test method was referred to Example 1. The results showed that the calcium content of the root and aboveground parts of peas was reduced, and the calcium content of the aboveground parts was even lower than that in Comparative Example 4. This shows that the lack of (NH4)6Mo7O 24 4H2O and H3BO3 affect the transport of calcium to the aboveground part of peas. Various nutrients interact with each other to a certain extent, and together they affect the transport of calcium to the aboveground part of peas.

[0070] With reference to Example 1, the fresh weight and dry weight of pea plants were measured and recorded. Nitrogen, molybdenum and boron are essential nutrients for plants. 24 4H2O and H3BO3 are not conducive to the accumulation of pea biomass. Therefore, the fresh weight and dry weight of the pea plants were reduced compared with the group of 5 μM NiCl2 in Example 1 and Comparative Example 4.

[0071] The above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit the scope of protection of the present invention. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that the technical solutions of the present invention may be modified or replaced by equivalents, but these modifications or replacements are all within the scope of protection of the present invention.

Claims

1. A method for promoting the transport of calcium to the aerial parts of plants, characterized in that: The specific steps are as follows: selecting plant germinated seeds that have grown roots with a root length of 2 to 6 cm, and during the growth process of the plant germinated seeds, The basic nutrient solution A includes the following ingredients: Ca(NO3)2·4H2O 2.5mM, KH2PO4 1mM, KCl50mM, MgSO4·7H2O 2mM, K2SO4 2mM, FeSO4·7H2O 0.1mM, EDTA·Na2 0.1mM, MnCl2 2μM, ZnSO4·7H2O 4μM, CuSO4·5H2O 0.5μM, (NH4)6Mo7O 24 ·4H2O 1μM, H3BO3 25μM, NiCl2 2~20μM; The basic nutrient solution B includes the following ingredients: Ca(NO3)2·4H2O 2.5mM, KH2PO4 1mM, KCl50mM, MgSO4·7H2O 2mM, K2SO4 2mM, FeSO4·7H2O 0.1mM, EDTA·Na2 0.1mM, MnCl2 2μM, ZnSO4·7H2O 4μM, CuSO4·5H2O 0.5μM, (NH4)6Mo7O 24 ·4H2O 1μM, H3BO3 25μM, NiCl2 2~20μM; Apply nutrients as follows: (1) For 1-3 days, a first nutrient solution is used, wherein the concentration of all components in the first nutrient solution is 20-30% of that in the basic nutrient solution A; (2) A second nutrient solution is used from 4 to 10 days, wherein the concentration of all components in the second nutrient solution is 20 to 30% of that in the basic nutrient solution B.

2. The method for promoting calcium transport to the aerial parts of plants according to claim 1, wherein: The plant germinated seeds may be derived from beans and cruciferous vegetables.

3. The method for promoting calcium transport to the aerial parts of plants according to claim 2, wherein: In the plant germinated seeds, the seed source includes pea.

4. The method for promoting calcium transport to the aerial parts of plants according to claim 1, wherein: The plant germinated seeds having grown roots with a root length of 3 to 4 cm are selected.

5. The method for promoting calcium transport to the aerial parts of plants according to claim 1, wherein: During the growth process of the plant germination seeds, the temperature is controlled at 20-27° C., and the humidity is controlled at 70%-80%.

6. The method for promoting calcium transport to the aerial parts of plants according to claim 1, wherein: During the growth of the plant germination seeds, light treatment is also applied: 16h light / 8h dark cycle; Light intensity is 60-250 μmol·m -2 ·s -1 .

7. The method for promoting calcium transport to the aerial parts of plants according to claim 1, wherein: In the basic nutrient solution B, the concentration of NiCl2 is 2-5 μM.

8. The method for promoting calcium transport to the aerial parts of plants according to claim 1, wherein: The pH of the first nutrient solution is 5-6; the pH of the second nutrient solution is 5-6.

9. The method for promoting calcium transport to the aerial parts of plants according to claim 1, wherein: The cultivation method for obtaining the plant germinated seeds includes at least one of hydroponics and soil cultivation.

10. The method for promoting calcium transport to the aerial parts of plants according to claim 1, wherein: Obtaining the plant germinated seeds comprises the following culture steps: Step 1: Soak the plant seeds in disinfectant and rinse with deionized water until there is no obvious pungent odor; Step 2: Soak the sterilized plant seeds in a dark environment at 20-26° C. for 8-15 hours, then transfer them to a seedling tray and culture them in a dark environment at 20-26° C. for 2-4 days until the roots grow to 3-4 cm.

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