Method for regulating and controlling nutrition enrichment of initial feed of aquatic products based on lipid metabolism and application of method

Abstract

The invention belongs to the technical field of aquaculture. The invention discloses a method for regulating and controlling nutrient enrichment of initial feed of aquatic products based on lipid metabolism. The method is characterized by comprising the following steps: 1) key fatty acid identification: respectively determining fatty acid compositions of target aquatic product larvae and fairy shrimp nauplii, and identifying unsaturated fatty acids which are relatively lacking in the fairy shrimp nauplii but have relatively high requirements on the target aquatic product larvae; (2) inspection of a strengthening source: detecting schizochytrium limacinum powder which is identified in the step (1) and lacks unsaturated fatty acid, and verifying the content of target unsaturated fatty acid in the schizochytrium limacinum powder; 3) bait nutrition enhancement: using schizochytrium limacinum powder as an enhancer for feeding fairy shrimp nauplii, and increasing the unsaturated fatty acid content of target aquatic product larvae; (4) intensified bait feeding and effect evaluation: feeding the nutrient-intensified fairy shrimp nauplii to the target aquatic product larvae, and evaluating the growth performance of the target aquatic product larvae and the nutrition improvement condition of muscular tissues. The method can effectively improve the nutritional value of the initial feed of the aquatic product.

Description

Technical Field

[0001] The present invention belongs to the technical field of aquaculture, and in particular relates to a method for enhancing the nutritional value of aquatic product starter feed based on lipid metabolism regulation and its application. Background Art

[0002] Starter feed is one of the most important factors affecting the growth and survival of fish larvae and juveniles. Fish rely on the nutrients in their yolk sac for early development. As the yolk sac gradually shrinks and their digestive systems fully develop, larvae begin to consume a mixed diet and enter a mixed nutrition phase. Choosing a palatable and nutritionally comprehensive starter feed at this stage directly determines the success or failure of large-scale fry production. Therefore, choosing the right starter feed is a crucial issue for fish farmers during fry rearing.

[0003] Artemia eggs are nutritious, easy to store and transport, and feature simple and convenient hatching. The hatched Artemia nauplii are rich in nutrients, including yolk, protein, amino acids, unsaturated fatty acids, minerals, trace elements, and vitamins, making them an excellent starter feed for fish and shrimp larvae and widely used in aquaculture. However, Artemia nauplii lack important polyunsaturated fatty acids, such as EPA and DHA, which are crucial for the neurodevelopment and overall growth of aquatic animal larvae. Deficiency can lead to growth stagnation and decreased immunity. To address these issues, Schizochytrium algae powder, rich in target fatty acids, was selected and added as a fortifier. This enhances lipid metabolism by reconstructing the exogenous-endogenous metabolic balance, intervening in the lipid metabolism, transport, and synthesis pathways of Artemia nauplii, and promoting their accumulation of polyunsaturated fatty acids. This improves the nutritional value of the starter feed, promoting the growth and development of juvenile fish, and improving their nutritional composition. Summary of the Invention

[0004] The purpose of the present invention is to provide a method for nutrient enhancement of aquatic product starter bait based on lipid metabolism regulation and its application, which can effectively improve the nutritional value of aquatic product starter bait.

[0005] To achieve the above objectives, the technical solution adopted by the present invention is: a method for nutritional enhancement of aquatic product starter feed based on lipid metabolism regulation, characterized by comprising the following steps: 1) identification of key fatty acids: respectively measuring the fatty acid composition of target aquatic product larvae (such as sea bass larvae) and artemia nauplii, and identifying unsaturated fatty acids that are relatively lacking in artemia nauplii but are in high demand by the target aquatic product larvae; 2) Testing of the enrichment source: Testing the Schizochytrium powder enriched with the unsaturated fatty acids identified in step 1) and verifying the target unsaturated fatty acid content; 3) Fortification of feed nutrition: Schizochytrium powder is used as a fortifier for feeding Artemia nauplii to increase the unsaturated fatty acid content of target aquatic product larvae (such as sea bass larvae); 4) Fortified feed feeding and effect evaluation: Feed the nutritionally fortified Artemia nauplii to target aquatic larvae (e.g. sea bass), and evaluate the nutritional improvement in the growth performance (weight, length, survival rate) and muscle tissue (especially the content of PUFA and the key fatty acid DHA) of the target aquatic larvae (e.g. sea bass).

[0006] Preferably, the unsaturated fatty acid identified as being deficient in Artemia nauplii in step 1) is 22:6n-3 fatty acid (DHA).

[0007] Preferably, the target aquatic product larvae in step 1) are sea bass larvae.

[0008] Preferably, the dosage of the Schizochytrium powder (enhancer) in step 3) is 10 mg / L-60 mg / L.

[0009] Preferably, in step 4), the target aquatic product larvae (e.g., sea bass juveniles) are fed with the following method: the amount of fortified Artemia nauplii added each time is 10%-15% of the total weight of the target aquatic product larvae (e.g., sea bass larvae, juveniles) to be tested, and the feeding frequency is 3 times per day, so as to increase the content of the fatty acids that are deficient in the target aquatic product larvae (e.g., sea bass juveniles).

[0010] Preferably, a method for nutritional enhancement of aquatic product starter feed based on lipid metabolism regulation is characterized by comprising the following specific steps: 1) Artemia incubation: Based on a density of 20 Artemia eggs / mL, incubate the eggs in a culture bottle filled with artificial seawater for 24 hours. The incubation temperature is approximately 25°C, the salinity is 25‰ (meaning 25 grams of salt per kilogram of seawater), and the pH is 8. Aeration is continuously performed using an aerator throughout the incubation process. 2) Separation of Artemia: Filter the hatched Artemia through 200-mesh and 500-mesh sieves, and collect the Artemia nauplii in the 500-mesh sieve; 3) Artemia fortification: Add 10-60 mg / L of Schizochytrium algae powder to artificial seawater (i.e., Schizochytrium algae powder: artificial seawater = 10-60 mg: 1 L). Three parallel groups of Artemia nauplii were cultured in culture bottles containing artificial seawater at a density of 10 Artemia nauplii / mL. All other conditions remained the same as incubation in step 1) {i.e., incubation temperature during step 1) was approximately 25°C, salinity 25‰, and pH 8}. The fortification period was 24 hours. 4) Artemia collection: The fortified Artemia nauplii were filtered through a 500-mesh sieve and the fortified Artemia nauplii were washed with distilled water. 5) Artemia index determination: The growth performance of the fortified Artemia nauplii is determined, including survival rate, body length, weight, and nutritional composition; the nutritional composition includes amino acid composition and fatty acid composition, to obtain aquatic product starter bait based on lipid metabolism regulation (or fortified Artemia nauplii bait).

[0011] The above-mentioned application of aquatic product open-mouth bait based on lipid metabolism regulation comprises the following steps: 1) Seabass rearing: Seabass juveniles were fed a lipid metabolism-modulated aquatic product starter feed (or enhanced Artemia nauplii feed) three times daily for 56 days. The amount of lipid metabolism-modulated aquatic product starter feed (or enhanced Artemia nauplii feed) added each time was 10%-15% of the total weight of the seabass juveniles (target aquatic product juveniles).

[0012] 2) Seabass Growth and Nutritional Assessment: Determine the growth performance of seabass, including body weight, body length, and survival rate; analyze the fatty acid composition of muscle, focusing on the evaluation of PUFA and DHA content.

[0013] The present invention selects juvenile seabass to carry out enhanced Artemia rearing experiments, which is the result of the combined effects of industrial value, seedling characteristics, scientific research needs and practical conditions. As a core profitable species in freshwater fisheries, the high economic status of seabass makes its juvenile breeding a key to the industry's "cost-quality-risk" management and control; its adaptability to starter baits, seedling breeding pain points, and key nutritional needs and metabolic research value in the juvenile stage provide exploration space for optimized breeding; the controllability of indoor breeding and the intuitiveness of growth observation ensure that the experiment is operational and easy to verify. Through this experiment, we can not only solve practical problems in the industry, but also deepen the research on aquatic nutrition and metabolism, promote the high-quality development of freshwater fisheries, and achieve an effective connection between industrial needs and academic exploration.

[0014] The beneficial effects of the present invention are: 1) This method can effectively improve the nutritional value of aquatic feed. By interfering with the lipid metabolism pathway of Artemia with Schizochytrium algae powder, this method achieves synergistic enrichment of DHA and essential amino acids in the feed, providing a "high-energy, high-nutrition" fortified feed for juvenile aquatic fish. This method fills a gap in the existing technology for precisely regulating lipid metabolism in aquatic feed nutrition, providing a new method for improving the quality and efficiency of the aquatic seedling industry, and has broad application prospects in the field of juvenile aquatic fish feeding.

[0015] 2) Easy to operate: No complex equipment is required, and it can be implemented in existing seedling facilities, making it suitable for large-scale production.

[0016] 3) Significant economic benefits: The growth rate of juvenile fish is increased, the DHA content in muscle is significantly increased, and the breeding cost is reduced.

[0017] 4) Technical versatility: The idea based on lipid metabolism regulation can be extended to the starter feed enhancement of other aquatic species (such as grouper and large yellow croaker), and has broad application prospects. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] Figure 1 Graph showing the survival rate (left) and body length (right) of Artemia of the present invention.

[0019] Figure 2 This is a diagram of the expression levels of the lipid metabolism regulatory pathway of the present invention.

[0020] Table 1 shows the free amino acid composition of fortified Artemia.

[0021] Table 2 shows the free fatty acid composition of enriched Artemia.

[0022] Table 3 shows the growth performance of sea bass juveniles.

[0023] Table 4 shows the free fatty acid composition of sea bass juveniles. DETAILED DESCRIPTION

[0024] In order to better understand the present invention, the content of the present invention is further illustrated below in conjunction with the examples, but the content of the present invention is not limited to the following examples.

[0025] Example 1 (control group, without Artemia reinforcement): The following steps are involved: 1) Artemia hatching: Based on a density of 20 Artemia eggs / mL, Artemia eggs were placed in a culture bottle filled with artificial seawater and incubated for 24 h. The incubation temperature was approximately 25°C, the salinity was 25‰, and the pH was 8. Aeration was continuously performed using an aerator throughout the incubation process.

[0026] 2) Separation of Artemia: Filter the hatched Artemia through 200-mesh and 500-mesh sieves, and collect the Artemia nauplii in the 500-mesh sieve.

[0027] The steps of filtering the hatched Artemia through different 200-mesh and 500-mesh sieves (the same as in the following embodiments) are as follows: a. Preliminary filtration: Use a 200-mesh sieve to preliminarily filter the hatching liquid to remove larger impurities and larger larvae; b. Fine filtration: The liquid after the initial filtration is filtered through a 500-mesh sieve for a second time to separate smaller larvae and impurities.

[0028] 3) Artemia index measurement: Growth performance including survival rate, body length, weight, nutritional composition including amino acid composition and fatty acid composition were measured, and differential lipid metabolism genes were screened by transcriptome sequencing and verified by RT-qPCR.

[0029] 4) Seabass rearing: Feed Artemia nauplii to seabass juveniles three times daily for 56 days. The amount of Artemia nauplii fed each time should be 12% of the total weight of the target aquatic product juveniles (e.g., seabass juveniles).

[0030] 5) Seabass Growth and Nutrition Assessment: Determination of growth performance (weight, length, and survival rate); analysis of muscle fatty acid composition, with a focus on PUFA and DHA content.

[0031] Example 2 (Experimental Group 1) A method for nutritional enhancement of aquatic product starter bait based on lipid metabolism regulation, comprising the following steps: 1) Artemia hatching: Based on a density of 20 Artemia eggs / mL, Artemia eggs were placed in a culture bottle filled with artificial seawater and incubated for 24 hours. The incubation temperature was approximately 25°C, the salinity was 25‰ (meaning 25 grams of salt per kilogram of seawater), and the pH was 8. Aeration was continuously performed using an aerator throughout the incubation process.

[0032] 2) Separation of Artemia: Filter the hatched Artemia through 200-mesh and 500-mesh sieves, and collect the Artemia nauplii in the 500-mesh sieve.

[0033] 3) Artemia fortification: 10 mg / L of Schizochytrium algae powder was added to artificial seawater (i.e., Schizochytrium algae powder: artificial seawater = 10 mg: 1 L). Three parallel groups of Artemia nauplii were cultured in culture bottles containing artificial seawater at a density of 10 Artemia nauplii / mL. All other conditions remained the same as in the incubation (i.e., incubation temperature approximately 25°C, salinity 25‰, pH 8) and fortification for 24 h. 4) Collection of Artemia: The fortified Artemia nauplii were filtered through a 500-mesh sieve to filter the water, and the fortified Artemia nauplii were washed with distilled water.

[0034] 5) Determination of Artemia indices: The growth performance of the fortified Artemia nauplii is measured, including survival rate, body length, weight, and nutritional composition; the nutritional composition includes amino acid composition and fatty acid composition, to obtain aquatic product starter feed based on lipid metabolism regulation (or fortified Artemia nauplii feed, i.e., fortified Artemia nauplii).

[0035] The above-mentioned application of aquatic product open-mouth bait based on lipid metabolism regulation comprises the following steps: 1) Seabass Rearing: Feed seabass juveniles with lipid metabolism-modulating aquatic product starter feed (or fortified Artemia nauplii feed, i.e., fortified Artemia nauplii) three times daily for 56 days. The amount of fortified Artemia nauplii (starter feed) added each time should be 12% of the total weight of the target aquatic product larvae (e.g., seabass juveniles and juveniles) for the assay.

[0036] 2) Seabass Growth and Nutritional Assessment: Determine the growth performance of seabass, including body weight, body length, and survival rate; analyze the fatty acid composition of muscle, focusing on the evaluation of PUFA and DHA content.

[0037] Example 3 (Experimental Group 2) A method for nutritional enhancement of aquatic product starter bait based on lipid metabolism regulation, comprising the following steps: 1) Artemia hatching: Based on a density of 20 Artemia eggs / mL, Artemia eggs were placed in a culture bottle filled with artificial seawater and incubated for 24 h. The incubation temperature was approximately 25°C, the salinity was 25‰, and the pH was 8. Aeration was continuously performed using an oxygen pump throughout the incubation process.

[0038] 2) Separation of Artemia: Filter the hatched Artemia through 200-mesh and 500-mesh sieves, and collect the Artemia nauplii in the 500-mesh sieve.

[0039] 3) Artemia fortification: 30 mg / L of Schizochytrium powder was added to artificial seawater. Artemia nauplii from each parallel group were cultured in culture bottles containing artificial seawater at a density of 10 nauplii / mL. Other conditions remained the same as in the incubation (i.e., incubation temperature of approximately 25°C, salinity of 25‰, and pH 8) in step 1). The fortification period was 24 h.

[0040] 4) Collection of Artemia: The fortified Artemia nauplii were filtered through a 500-mesh sieve to filter the water, and the fortified Artemia nauplii were washed with distilled water.

[0041] 5) Determination of Artemia indices: Determination of growth performance of fortified Artemia nauplii, including survival rate, body length, body weight, and nutritional composition. The nutritional composition includes amino acid composition and fatty acid composition, to obtain aquatic product starter feed based on lipid metabolism regulation (or fortified Artemia nauplii feed, i.e., fortified Artemia nauplii).

[0042] The above-mentioned application of aquatic product open-mouth bait based on lipid metabolism regulation comprises the following steps: 1) Seabass rearing: Seabass juveniles were fed a lipid metabolism-regulated aquatic product starter feed (also known as fortified Artemia nauplii feed, i.e., fortified Artemia nauplii) three times daily for 56 days. The amount of fortified Artemia nauplii (starter feed) added each time was 15% of the total weight of the seabass juveniles.

[0043] 2) Seabass growth and nutritional assessment: Determination of growth performance (weight, length, and survival rate); analysis of muscle fatty acid composition, with a focus on PUFA and DHA content.

[0044] Example 4 (Experimental Group 3) A method for nutritional enhancement of aquatic product starter bait based on lipid metabolism regulation, comprising the following steps: 1) Artemia hatching: Based on a density of 20 Artemia eggs / mL, Artemia eggs were placed in a culture bottle filled with artificial seawater and incubated for 24 h. The incubation temperature was approximately 25°C, the salinity was 25‰, and the pH was 8. Aeration was continuously performed using an aerator throughout the incubation process.

[0045] 2) Separation of Artemia: Filter the hatched Artemia through 200-mesh and 500-mesh sieves, and collect the Artemia nauplii in the 500-mesh sieve.

[0046] 3) Artemia fortification: 60 mg / L of Schizochytrium powder was added to artificial seawater. Three parallel groups of Artemia nauplii were cultured in culture bottles containing artificial seawater at a density of 10 nauplii / mL. Other conditions remained the same as in the incubation (i.e., incubation temperature of approximately 25°C, salinity of 25‰, and pH 8) for 24 h.

[0047] 4) Collection of Artemia: The fortified Artemia nauplii are filtered through a sieve and washed with distilled water.

[0048] 5) Artemia index determination: The growth performance of the fortified Artemia nauplii was measured, including survival rate, body length, weight, and nutritional composition. The nutritional composition included amino acid composition and fatty acid composition. Transcriptome sequencing was used to screen for differentially expressed genes in lipid metabolism and verified by RT-qPCR. The lipid metabolism-regulated aquatic product starter feed (or fortified Artemia nauplii feed, i.e., fortified Artemia nauplii) was obtained.

[0049] The above-mentioned application of aquatic product open-mouth bait based on lipid metabolism regulation comprises the following steps: 1) Seabass rearing: Seabass juveniles were fed a lipid metabolism-regulated aquatic product starter feed (also known as fortified Artemia nauplii feed, i.e., fortified Artemia nauplii) three times daily for 56 days. The amount of fortified Artemia nauplii (starter feed) added each time was 12% of the total weight of the seabass juveniles.

[0050] 2) Seabass growth and nutritional assessment: Determination of growth performance (weight, length, and survival rate); analysis of muscle fatty acid composition, with a focus on PUFA and DHA content.

[0051] according to Figure 1 The dose of Schizochytrium algae powder had no significant effect on the survival rate and body length of Artemia. The 0 mg / L group without algae powder supplementation showed the most rapid decline in survival; the 10 and 30 mg / L groups maintained stable survival, but the enhancement effect was limited. Survival was actually reduced at the high concentration of 60 mg / L, possibly due to negative effects on Artemia's physiology, environment, and nutrient utilization. High concentrations of algae powder may place an increased metabolic burden on Artemia. For example, Schizochytrium algae powder contains complex components (lipids, polysaccharides, etc.). At 60 mg / L, the ingestion, digestion, and metabolism of these substances are extremely stressful for Artemia, exceeding its physiological capacity and potentially causing metabolic disturbances, affecting survival and growth, and hindering the realization of any advantages in survival rate and body length growth. Furthermore, high doses of algae powder may cause abnormalities in water quality, including dissolved oxygen, pH, and osmotic pressure. Algae powder aggregation and sedimentation may also disrupt Artemia's habitat, interfering with normal feeding and swimming, and weakening the enhancement effect. In terms of nutrient utilization, Artemia has an adaptive range of nutritional requirements for Schizochytrium algae powder. The nutritional components of the 60mg / L group may exceed its needs. For example, excessive intake of certain components may inhibit the absorption of other nutrients, or produce antagonistic effects in metabolism. As a result, although there is an algae powder enhancement effect, the efficiency of nutrient utilization is reduced, and the survival rate and growth indicators cannot be effectively improved.

[0052] As shown in Table 1, the total amino acid content (∑EAA) increased significantly with the addition of Schizochytrium meal at 10 mg / L and 60 mg / L, indicating that the addition of Schizochytrium meal helped to increase the essential amino acid content in Artemia. Arginine, at 10 mg / L and 60 mg / L, reached 10.09±0.91 mg / L and 10.06±0.36 mg / L, respectively, more than double the control (4.43±0.2 mg / L), making it the primary contributor to the increase in ∑EAA. The total non-essential amino acid content (∑NEAA) decreased after the addition of Schizochytrium meal, with significant decreases in aspartic acid and glutamic acid, suggesting a possible shift in metabolic flux from NEAA to EAA. This study reveals a mechanism by which Artemia ingests Schizochytrium meal to reshape its amino acid composition by regulating carbon and nitrogen metabolism, providing a target for bionutrient enhancement of feed.

[0053] Table 2 shows that the addition of Schizochytrium meal significantly affected the fatty acid composition of Artemia, particularly increasing DHA content while decreasing EPA content. Overall, the addition of Schizochytrium meal increased the content of polyunsaturated fatty acids, particularly DHA, in Artemia, but may have inhibited EPA accumulation. Furthermore, DHA content increased in a dose-dependent manner with increasing Schizochytrium meal addition, reaching the highest level in the 60 mg / L group. The significant enrichment of DHA content in the experimental Artemia groups may be due, on the one hand, to the direct accumulation of DHA through ingestion of Schizochytrium meal, while the observed decrease in EPA content may also be due to the conversion of EPA to DHA by desaturases and elongases, resulting in a significant increase in DHA content and a significant decrease in EPA content.

[0054] according to Figure 2 60 mg / L Schizochytrium powder significantly downregulated the expression of six key genes in Artemia: Pl (triglyceride lipase), Fatp1 / 4 (fatty acid transporter), Spla2 (phospholipase A2), D7s5d (sterol desaturase), Cyp3A (sterol oxidase), and Hsd17b2 (hydroxysteroid dehydrogenase), synergistically inhibiting the breakdown and utilization of endogenous lipids. Downregulation of Pl and Fatp1 / 4 reduced triglyceride hydrolysis and fatty acid transport, while decreased Spla2 expression maintained membrane phospholipid stability. Simultaneously, sterol metabolic flux was blocked, manifested by D7s5d-mediated inhibition of cholesterol synthesis, weakened Cyp3A-driven sterol oxidation, and delayed Hsd17b2-related steroid hormone metabolism. Ultimately, this reshaped lipid metabolism, shifting the worm's lipid mobilization away from endogenous lipids and toward efficient assimilation of exogenous lipids, ultimately enhancing its nutritional profile.

[0055] According to Table 3, Schizochytrium powder supplemented with Artemia can increase the weight and length of sea bass juveniles. There was no significant difference in the effects between the 10 mg / L and 30 mg / L groups, while the 60 mg / L group was significantly higher than the control group, indicating that high doses can significantly improve the growth performance of juveniles.

[0056] Table 4 shows that juveniles fed Schizochytrium meal supplemented with Artemia significantly increased their PUFA and DHA content compared to the control group. DHA and EPA are essential fatty acids for fish and directly promote protein synthesis. Their intake levels can affect the growth performance of juveniles. The 60 mg / L group achieved a total DHA + EPA content of 15.65%, a 2.48% increase compared to the control group, corresponding to a significant improvement in growth performance. The 60 mg / L group significantly improved juvenile growth by increasing DHA, EPA, ARA, and total PUFA without compromising survival.

[0057] Table 1 Free amino acid composition of Artemia (mg / 100g) Table 2 Free fatty acid composition of Artemia (%) Table 3 Growth performance of juvenile seabass Table 4 Free fatty acid composition of seabass juveniles (%)

Claims

1. A method for nutritional enhancement of aquatic product starter bait based on lipid metabolism regulation, characterized in that The following steps are involved: 1) Identification of key fatty acids: Determine the fatty acid composition of target aquatic product larvae and Artemia nauplii, respectively, to identify unsaturated fatty acids that are relatively deficient in Artemia nauplii but are in high demand by target aquatic product larvae; 2) Testing of the enrichment source: Testing the Schizochytrium powder enriched with the unsaturated fatty acids identified in step 1) and verifying the target unsaturated fatty acid content; 3) Fortification of feed nutrition: Schizochytrium algae powder is used as a fortifier for feeding Artemia nauplii to increase the unsaturated fatty acid content of the target aquatic product larvae; 4) Fortified feed feeding and effect evaluation: Feed the nutritionally fortified Artemia nauplii to the target aquatic product larvae, and evaluate the growth performance and nutritional improvement of the target aquatic product larvae and muscle tissue.

2. The method for nutritional enhancement of aquatic product starter feed based on lipid metabolism regulation according to claim 1, characterized in that: In step 1), it was determined that the unsaturated fatty acid lacking in Artemia nauplii is 22:6n-3 fatty acid (DHA).

3. The method for nutritional enhancement of aquatic product starter feed based on lipid metabolism regulation according to claim 1, characterized in that: In step 1), the target aquatic product larvae are sea bass larvae.

4. The method for nutritional enhancement of aquatic product starter feed based on lipid metabolism regulation according to claim 1, characterized in that: Step 3) The dosage of the Schizochytrium powder is 10 mg / L-60 mg / L.

5. The method for nutritional enhancement of aquatic product starter feed based on lipid metabolism regulation according to claim 1, characterized in that: The method of feeding the target aquatic product larvae in step 4) is as follows: the amount of fortified Artemia nauplii added each time is 10%-15% of the total weight of the target aquatic product larvae, and the number of times of feeding is 3 times per day, so as to increase the content of the fatty acids that are deficient in the target aquatic product larvae.

6. The method for nutritional enhancement of aquatic product starter feed based on lipid metabolism regulation according to claim 1, characterized in that: In step 4), the target growth performance of aquatic product larvae is weight, body length, and survival rate, and the muscle tissue is the content of PUFA and the key fatty acid DHA.

7. The method for nutritional enhancement of aquatic product starter feed based on lipid metabolism regulation according to claim 1, characterized in that: The specific steps include: 1) Artemia incubation: Based on a density of 20 Artemia eggs / mL, incubate the eggs in a culture bottle filled with artificial seawater for 24 hours at a temperature of approximately 25°C, a salinity of 25‰, and a pH of 8. Aeration was continuously performed using an aerator throughout the incubation process. 2) Separation of Artemia: Filter the hatched Artemia through 200-mesh and 500-mesh sieves, and collect the Artemia nauplii in the 500-mesh sieve; 3) Artemia fortification: Add 10-60 mg / L of Schizochytrium powder to artificial seawater. Culture Artemia nauplii in culture bottles containing artificial seawater at a density of 10 nauplii / mL. Other conditions remain the same as incubation in step 1). Fortification is for 24 hours. 4) Artemia collection: The fortified Artemia nauplii were filtered through a 500-mesh sieve and the fortified Artemia nauplii were washed with distilled water. 5) Artemia index measurement: The growth performance of the enriched Artemia nauplii was measured, including survival rate, body length, weight, and nutritional composition; The nutritional composition includes amino acid composition and fatty acid composition, and the aquatic product starter feed is obtained based on lipid metabolism regulation.

8. The method for nutritional enhancement of aquatic product starter feed based on lipid metabolism regulation according to claim 7, characterized in that: In step 3), the remaining conditions remain the same as those in step 1): the temperature during incubation is about 25°C, the salinity is 25‰, and the pH is 8.

9. The use of aquatic product starter bait based on lipid metabolism regulation according to claim 7, characterized in that The following steps are involved: 1) Seabass rearing: Feeding seabass juveniles with lipid metabolism-regulated aquatic product starter feed three times daily for 56 days. The amount of lipid metabolism-regulated aquatic product starter feed each time was 10%-15% of the total weight of the seabass juveniles. 2) Seabass Growth and Nutritional Assessment: Determine the growth performance of seabass, including body weight, body length, and survival rate; analyze the fatty acid composition of muscle, focusing on the evaluation of PUFA and DHA content.

Images