Traditional Chinese medicine composition for consolidating body resistance and resisting aging and preparation method thereof

Abstract

The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to a foundation-consolidating and anti-aging traditional Chinese medicine composition and a preparation method thereof. The traditional Chinese medicine composition is prepared from corn stigma, semen cuscutae, oysters, dogwood, gordon euryale seeds, white poria cocos, radix asparagi, lotus seeds, polygala tenuifolia pulp, ophiopogon roots, prepared rehmannia roots, schisandra chinensis, radix morindae officinalis, semen plantaginis, common yam rhizomes, fructus lycii, radix codonopsis, costus roots, broussonetia papyrifera leaves, platycladi seeds, raspberries, Chinese dates, ginseng and salvia miltiorrhiza. The aging process can be slowed down, and the service life can be prolonged.

Description

Technical Field

[0001] The present invention belongs to the technical field of traditional Chinese medicine, and particularly relates to a traditional Chinese medicine composition for strengthening the body and resisting aging and a preparation method thereof. Background Art

[0002] The information disclosed in this background technology section is only intended to enhance understanding of the overall background of the invention and should not necessarily be regarded as an admission or any form of suggestion that the information constitutes the prior art already known to those skilled in the art.

[0003] Aging is a natural physiological process, the result of the gradual loss of cell and tissue function over the long term. With age, the body's metabolic, immune, and endocrine systems gradually decline, leading to a gradual decline in tissue and organ function, which in turn can lead to various age-related diseases such as cardiovascular disease, diabetes, and osteoporosis. The mechanisms of aging involve multiple factors, including oxidative stress, free radical damage, gene mutations, endocrine disorders, and apoptosis.

[0004] Traditional Chinese Medicine (TCM) theory holds that aging is caused by an imbalance of yin and yang, insufficient qi and blood, and decreased organ function. Traditional Chinese medicine (TCM) can effectively slow the aging process by regulating qi and blood, nourishing yin and tonifying the kidneys, and promoting blood circulation and removing blood stasis. The advantage of TCM lies in its holistic approach, which improves body functions through multiple actions, thereby achieving anti-aging effects. In recent years, with the advancement of modern science and technology, an increasing number of TCM ingredients have been found to possess significant anti-aging properties. For example, herbs such as ginseng, wolfberry, astragalus, and ganoderma have been shown to enhance immunity, provide antioxidant benefits, and slow cellular aging. Furthermore, research on TCM formulas continues to deepen, with many traditional TCM formulas found to have promising anti-aging effects.

[0005] Chinese patent publication number CN1491714A discloses a Chinese medicine composition made from Chinese medicines including lotus seeds, gorgon fruit, yam, poria, wolfberry, white hyacinth bean, mulberry, rehmannia root, ginseng, cordyceps sinensis, and rhodiola rosea. It has anti-aging and strengthening effects, is suitable for sub-healthy people and people with sexual disorders, and can significantly enhance human immunity.

[0006] The Chinese patent with publication number CN106362005A discloses a sugar-free anti-aging oral liquid, which includes 12-18 parts of ginseng, 12-18 parts of wolfberry, 8-13 parts of Chinese yam, 6-10 parts of Schisandra chinensis, 12-18 parts of asparagus, 12-18 parts of ophiopogon, 12-18 parts of raw rehmannia, 12-18 parts of cooked rehmannia. 1-2 parts of celery root, 18-28 parts of rhizome of Chinese holly, 12-18 parts of tuckahoe, 12-18 parts of cyperus rotundus, 12-18 parts of angelica sinensis, 12-18 parts of psoralea corylifolia, 12-18 parts of eucommia bark, 12-18 parts of morinda officinalis, 10-15 parts of anemarrhena, 10-15 parts of phellodendron chinense, 5-10 parts of curculigo orchioides, 5-10 parts of epimedium, 18-28 parts of oriental dwarfism, 12-18 parts of salvia miltiorrhiza, 12-18 parts of astragalus, 12-18 parts of mistletoe, 15-20 parts of glossy privet fruit, 16-24 parts of eclipta prostrata, 5-8 parts of cinnamon bark, 8-12 parts of dendrobium officinale, 0.2-3 parts of sweetener, 0.5-2 parts of ethylparaben and 1-3 parts of sodium benzoate.

[0007] Although traditional Chinese medicine has made some progress in the field of anti-aging, the existing anti-aging traditional Chinese medicine preparations still have the following problems: (1) Insufficient validation of efficacy: Many traditional Chinese medicine anti-aging prescriptions lack systematic validation and evaluation of their efficacy during application, which affects the credibility of their widespread application.

[0008] (2) Poor efficacy: Traditional Chinese medicine preparations do not fully extract their active ingredients, resulting in poor effects. The extraction method needs to be further improved. Summary of the Invention

[0009] In order to overcome the shortcomings of the existing technology, the present invention provides a traditional Chinese medicine composition. The traditional Chinese medicine composition of the present invention is designed mainly for anti-aging, prolonging life, and enhancing immunity. Through the comprehensive effect of multiple medicinal materials, it can achieve the effects of strengthening physical fitness, improving immunity, nourishing yin and kidney, calming the mind and stabilizing the spirit.

[0010] The formula of the Chinese medicine composition of the present invention is as follows: 150-250 parts by weight of corn silk, 350-450 parts by weight of dodder seed, 180-220 parts by weight of oyster, 170-230 parts by weight of cornus officinalis, 250-350 parts by weight of euryale ferox, 150-250 parts by weight of white poria, 75-120 parts by weight of asparagus root, 180-240 parts by weight of lotus seed, 90-130 parts by weight of polygala root, 80-130 parts by weight of ophiopogon root, 90-140 parts by weight of prepared rehmannia root, 50-1 50 weight parts, Morinda officinalis 35-85 weight parts, Plantago seed 110-180 weight parts, Chinese yam 75-125 weight parts, wolfberry fruit 70-150 weight parts, Codonopsis pilosula 80-125 weight parts, Magnolia officinalis 50-110 weight parts, Broussonetia mulberry leaves 125-170 weight parts, Platycladus orientalis seeds 80-130 weight parts, Raspberry 120-180 weight parts, jujube (without kernel) 350-430 weight parts, ginseng 150-240 weight parts, and Salvia miltiorrhiza 80-125 weight parts.

[0011] Analysis of monarch, minister, assistant and envoy: King medicine: Ginseng: Ginseng greatly replenishes vital energy, strengthens lung qi, and benefits spleen qi. It is the most important medicine for replenishing qi. It can enhance immunity, boost the function of internal organs, and is the basis for delaying aging (sufficient qi promotes blood circulation and consolidates yin and yang).

[0012] Plantago: It can clear away heat, promote diuresis, and relieve stranguria. It can dredge the water channels, expel dampness and turbidity, and prevent dampness from blocking the circulation of qi and blood. It is the core of "dredge and promote urination", so that it can replenish without stagnation, and take into account the dampness and turbidity removal in the "harmony of yin and yang".

[0013] Paper mulberry leaves: Traditional records show that the leaves of the mulberry tree have the effects of clearing heat and dampness, cooling blood and stopping bleeding, and replenishing deficiency. Modern research suggests that it contains a variety of active ingredients that can help regulate metabolism, enhance immunity, and synergize with plantago seeds to eliminate dampness while also replenishing deficiency. It is one of the main medicines for "clearing and replenishing combined".

[0014] The combined effect of the main medicines: with tonifying qi and strengthening the spleen as the core, while taking into account the elimination of dampness and turbidity, it not only replenishes the source of qi and blood biochemical metabolism, but also promotes the flow of qi, laying the foundation for "regulating qi, blood, yin and yang, and enhancing immunity."

[0015] Minister medicine: Cuscuta, raspberry, cornus officinalis, schisandra chinensis: Cuscuta chinensis nourishes the kidneys and improves essence, nourishes the liver and improves eyesight, raspberry nourishes the kidneys and consolidates essence, Cornus officinalis nourishes the liver and kidneys, astringes essence and qi, and Schisandra chinensis nourishes the lungs and kidneys, invigorates qi and produces body fluid. All four herbs enter the liver and kidneys, assisting the main herbs in enhancing the effect of "tonifying yin and benefiting yang", especially strengthening the function of tonifying the kidneys and consolidating essence (kidneys are the foundation of congenitality, and kidney deficiency is the core pathogenesis of aging), helping to delay aging.

[0016] Zhengfangdang (equal parts of Astragalus, Saposhnikovia divaricata, and Codonopsis pilosula) Astragalus replenishes Qi and raises Yang, consolidates the exterior and stops sweating, while Saposhnikovia dispels wind and relieves exterior symptoms, and Codonopsis replenishes Qi and strengthens the spleen. The three work together to enhance the power of replenishing Qi and consolidating the exterior, assisting ginseng and Codonopsis in strengthening immunity ("when righteous Qi exists within, evil cannot invade"), while also dispelling wind and preventing evil, and are important aids in "enhancing immunity."

[0017] Jujube: It can tonify the middle and replenish qi, nourish blood and calm the mind. Its usage in large dosage can not only assist ginseng and codonopsis in replenishing qi, but also nourish blood, harmonize qi and blood, and at the same time alleviate the medicinal properties. It is an important adjuvant for "regulating qi and blood".

[0018] White Poria It can strengthen the spleen and eliminate dampness, calm the mind and soothe the nerves. It can not only assist ginseng and codonopsis in strengthening the spleen (enhancing the biochemical transformation of qi and blood), but also cooperate with plantago seed in eliminating dampness (strengthening the elimination of turbidity), and can also calm the mind to help "peace of mind". It is the key ministerial medicine connecting "tonification" and "communication", and makes up for the synergistic effect of the monarch medicine in eliminating dampness and strengthening the spleen.

[0019] Adjuvant: Yin-tonifying and fluid-invigorating herbs: Asparagus cochinchinensis, Radix Ophiopogonis, Rehmannia root, and Lycium barbarum Asparagus cochinchinensis and Ophiopogon japonicus nourish yin and moisten dryness, Rehmannia root replenishes blood and yin, benefits essence and fills marrow, wolfberry nourishes liver and kidney, benefits essence and improves eyesight, assists the yang-tonifying drugs in the main and auxiliary medicines, avoids damage to yin by warm and dryness, balances "yin and yang", and at the same time nourishes yin to nourish qi and blood (yin and blood have the same origin).

[0020] Spleen-strengthening and astringent: Euryale ferox, lotus seeds, Chinese yam Gorgon fruit and lotus seeds strengthen the spleen and stop diarrhea, benefit the kidneys and consolidate essence, while Chinese yam nourishes the spleen and stomach, promotes fluid production and benefits the lungs, nourishes the kidneys and astringes essence. They assist ginseng and codonopsis in strengthening the spleen, while enhancing the ability to consolidate the kidneys and astringe essence, taking into account "double nourishment of the spleen and kidneys" (the spleen is the foundation of acquired constitution, and the kidneys are the foundation of innate constitution, and the two nourish qi and blood together).

[0021] Calming and relieving depression: Polygala root, cypress seed Polygala tenuifolia calms the mind, improves intelligence, eliminates phlegm and opens the orifices, while cypress seed nourishes the heart and soothes the mind, moistens the intestines and promotes bowel movements. Both of them are used as adjuvants for blood-nourishing herbs (such as jujube and Rehmannia glutinosa) to harmonize the mind, achieving both "harmony of qi and blood" and "peace of mind" (the "co-nurturing of body and spirit" in Traditional Chinese Medicine is the key to delaying aging).

[0022] Qi-regulating and blood-activating herbs: Guangmuxiang, Danshen Guangmuxiang promotes qi and relieves pain, strengthens the spleen and aids digestion, and controls the greasy nature of tonic medicines such as ginseng, astragalus, and jujube, making them nourishing without being stagnant; Danshen promotes blood circulation and removes blood stasis, nourishes blood and calms the mind, and assists in "regulating qi and blood", making qi and blood circulation smoother and avoiding blood stasis caused by excessive qi replenishment.

[0023] Astringent: Oyster Oysters can calm the mind, soothe the liver and suppress yang, and have astringent and firming effects. They can assist in controlling the ascending and dispersing properties of yang-tonifying drugs, while also enhancing the ability to consolidate the kidneys and astringe essence, while also calming the mind and balancing yin and yang.

[0024] Drug: corn silk It can promote diuresis and reduce swelling, eliminate dampness and reduce jaundice, and has a mild nature and flavor. It assists Plantago seed and Broussonetia melaleuca leaves in removing dampness, while at the same time alleviating the heavy nature of the tonic medicine in the whole formula, allowing the medicinal properties to flow smoothly and playing a "harmony" role.

[0025] Morinda officinalis It can replenish kidney yang and strengthen muscles and bones. It is used in small dosages and can assist with yang-tonifying drugs such as Cuscuta chinensis. At the same time, its warming and unblocking nature can guide other drugs into the kidney meridian, acting as a "channel-guiding" agent.

[0026] The formula is based on the principles of "tonifying Qi and strengthening the spleen (acquired nourishment)"—eliminating dampness and unblocking stagnation (unblocking Qi flow)—tonifying the kidneys and consolidating essence (innate nourishment)—and nourishing Yin and calming the mind (regulating balance). Ginseng, jujube, and Poria are used to nourish the spleen and stomach, ensuring the active biochemical production of Qi and blood. Plantago seed, Broussonetia melanocarpa leaves, and corn silk are used to eliminate dampness and turbidity, preventing stagnation. Cuscuta seed, Cornus officinalis, and Rehmannia root are used to tonify the kidneys and replenish essence, delaying kidney essence deficiency. Aucklandia lappa and Salvia miltiorrhiza regulate Qi and activate blood circulation, preventing stagnation without replenishing. Polygala tenuifolia and Platycladus orientalis calm the mind, nurturing both the body and the spirit. Ultimately, the formula achieves "full Qi and blood, balanced Yin and Yang, cleared dampness and turbidity, consolidated kidney essence, and a tranquil mind," thus achieving the combined effects of "regulating Qi, blood, Yin and Yang, enhancing immunity, and delaying aging."

[0027] The preparation method of the Chinese medicine composition of the present invention is: Step a: Grind ginseng and paper mulberry leaves into fine powder, pass through a 300-mesh sieve to obtain medicinal powder, and set aside; Step b: soaking the plantago seed in an aqueous solution containing 5.0% to 10.0% amino acids (glycine, tyrosine, threonine, serine, cysteine, and L-aspartic acid) by mass for 0.5 to 1.5 hours, washing, adding 3 to 6 times the volume of 75% to 85% ethanol for extraction for 0.5 to 1.5 hours, adding 0.01 to 0.05 times the amount of vitamin E in the plantago seed, refluxing in a water bath at 55° C. to 65° C. for 1.5 to 2.5 hours, filtering, collecting the filtrate, and performing vacuum rotary evaporation at 45° C. to remove ethanol, filtering, and obtaining a plantago seed extract; Step c: after processing corn silk, dodder seed, oyster, cornus officinalis, euryale ferox, white poria, asparagus, lotus seed, polygala root, ophiopogon, prepared rehmannia root, schisandra chinensis, moringa officinalis, yam, wolfberry, codonopsis pilosula, costus root, cypress seed, raspberry, jujube, and salvia miltiorrhiza, add 6 to 8 times water and boil for 0.5 to 1.5 hours, filter, and set aside the filtrate; add 4 to 6 times water to the filter residue and boil for 1.5 to 2.5 hours, filter, collect the filtrate, combine the two filtrates, and concentrate to a relative density of 1.25 to 1.45 to obtain an aqueous extract for later use; Step d: uniformly mix the water extract, the plantago seed extract and the medicinal powder, add an appropriate amount of flavoring agent and / or filler, mix well, and prepare pills.

[0028] The present invention also provides use of the Chinese medicine composition of the present invention in preparing anti-aging drugs.

[0029] The present invention also provides use of the Chinese medicine composition of the present invention in preparing a drug for improving immunity.

[0030] Compared with the prior art, the technical effects of the present invention are: The Chinese medicine composition of the present invention can extend the telomeres of elderly mice or delay their shortening, which helps to slow down the aging process and prolong life. In addition, it can also increase the content of immunoglobulins (IgA, IgG, IgM) and cytokines (such as IL-2), which helps to improve the function of the immune system and overall immunity.

[0031] The present invention also optimizes the extraction process, and through further extraction of the plantago seed medicinal material, increases the content of the effective ingredient, which helps to improve the efficacy of the medicine. BRIEF DESCRIPTION OF THE DRAWINGS

[0032] Figure 1 : Extraction effect of glycine aqueous solution with different mass fractions.

[0033] Figure 2 : Extraction effect of threonine aqueous solution with different mass fractions.

[0034] Figure 3 : Relative telomere length of rats in each group.

[0035] Figure 4 : The expression of cytokine (IL-2) in the serum of rats in each group. DETAILED DESCRIPTION

[0036] In order to make the purpose and technical solution of the present invention more clear, the present invention is further described below in conjunction with the embodiments, but the scope of protection of the present invention is not limited to these embodiments, and the embodiments are only used to illustrate the present invention. It should be understood by those skilled in the art that any changes or equivalent substitutions that do not deviate from the concept of the present invention are included in the scope of protection of the present invention.

[0037] Optimization of the preparation method of Plantago asiatica extract The quality of Plantago seed extract is calculated based on the content of geniposide and verbascoside. The determination method of geniposide and verbascoside is based on the high performance liquid chromatography method (General Chapter 0512) in the Chinese Pharmacopoeia: Chromatographic conditions and system suitability test: Use octadecylsilane bonded silica as the filler; methanol as mobile phase A, 0.5% acetic acid solution as mobile phase B, with gradient elution as specified in the table below; detection wavelength is 254 nm. The theoretical plate number calculated based on the geniposide peak should be no less than 3000.

[0038] Table 1 Mobile phase elution program Preparation of reference solution: Take appropriate amount of geniposide reference substance and verbascoside reference substance, accurately weigh them, place them in a brown volumetric flask, and add 60% methanol to make a mixed solution containing 0.1 mg of each per 1 ml.

[0039] Preparation of test solution: Take about 1g of the powder of this product (passed through No. 2 sieve), accurately weigh it, place it in a stoppered conical flask, accurately add 50ml of 60% methanol, weigh the weight, heat and reflux for 2 hours, cool, weigh it again, make up the lost weight with 60% methanol, shake well, filter, and take the filtrate to obtain.

[0040] Determination method: Accurately aspirate 10 μl of reference solution and test solution respectively, inject into liquid chromatograph, and determine.

[0041] Optimization screening of amino acid species Scheme A: Soak the plantago seed in an 8.0% glycine aqueous solution for 1 hour, wash, add 5 times the volume of 80% ethanol and extract for 1 hour, add 0.03 times the amount of vitamin E of the plantago seed, reflux extraction in a water bath at 60°C for 2 hours, filter, collect the filtrate, remove the ethanol by vacuum rotary evaporation at 45°C~60°C, filter, and obtain the plantago seed extract.

[0042] Scheme B: Soak the plantago seed in an 8.0% tyrosine aqueous solution for 1 hour, wash, add 5 times the volume of 80% ethanol and extract for 1 hour, add 0.03 times the amount of vitamin E of the plantago seed, reflux extraction in a water bath at 60°C for 2 hours, filter, collect the filtrate, remove the ethanol by vacuum rotary evaporation at 45°C~60°C, filter, and obtain the plantago seed extract.

[0043] Scheme C: Soak the plantago seed in an 8.0% threonine aqueous solution for 1 hour, wash, add 5 times the volume of 80% ethanol and extract for 1 hour, add 0.03 times the amount of vitamin E of the plantago seed, reflux extraction in a water bath at 60°C for 2 hours, filter, collect the filtrate, remove the ethanol by vacuum rotary evaporation at 45°C~60°C, filter, and obtain the plantago seed extract.

[0044] Scheme D: Soak the plantago seed in an 8.0% serine aqueous solution for 1 hour, wash, add 5 times the volume of 80% ethanol and extract for 1 hour, add 0.03 times the amount of vitamin E of the plantago seed, reflux extraction in a water bath at 60°C for 2 hours, filter, collect the filtrate, remove the ethanol by vacuum rotary evaporation at 45°C~60°C, filter, and obtain the plantago seed extract.

[0045] Scheme E: Soak the plantago seed in an 8.0% cysteine ​​aqueous solution for 1 hour, wash, add 5 times the volume of 80% ethanol and extract for 1 hour, add 0.03 times the amount of vitamin E of the plantago seed, reflux extraction in a water bath at 60°C for 2 hours, filter, collect the filtrate, remove the ethanol by vacuum rotary evaporation at 45°C~60°C, filter, and obtain the plantago seed extract.

[0046] Scheme F: Soak the plantago seed in an 8.0% by mass L-aspartic acid aqueous solution for 1 hour, wash, add 5 times the volume of 80% ethanol and extract for 1 hour, add 0.03 times the amount of vitamin E of the plantago seed, reflux extraction in a water bath at 60°C for 2 hours, filter, collect the filtrate, remove the ethanol by vacuum rotary evaporation at 45°C~60°C, filter, and obtain the plantago seed extract.

[0047] Scheme G: Soak the plantago seed in an 8.0% by mass alanine ethanol (95%) solution for 1 hour, wash, add 5 times the volume of 80% ethanol and extract for 1 hour, add 0.03 times the amount of vitamin E of the plantago seed, reflux extraction in a water bath at 60°C for 2 hours, filter, collect the filtrate, remove the ethanol by vacuum rotary evaporation at 45°C~60°C, filter, and obtain the plantago seed extract.

[0048] Scheme H: Soak the plantago seeds in a leucine ethanol (95%) solution with a mass fraction of 8.0% for 1 hour, wash, add 5 times the volume of 80% ethanol and extract for 1 hour, add 0.03 times the amount of vitamin E of the plantago seeds, reflux in a water bath at 60°C for 2 hours, filter, collect the filtrate, remove the ethanol by vacuum rotary evaporation at 45°C~60°C, filter, and obtain the plantago seed extract.

[0049] Scheme I: Soak the Plantago seeds in an ethanol (95%) solution for 1 hour, wash, add 5 times the volume of 80% ethanol and extract for 1 hour, add 0.03 times the amount of vitamin E of the Plantago seeds, reflux in a water bath at 60°C for 2 hours, filter, collect the filtrate, remove the ethanol by vacuum rotary evaporation at 45°C~60°C, filter, and obtain the Plantago seed extract.

[0050] Scheme J: Soak the Plantago seeds in deionized water for 1 hour, wash, add 5 times the volume of 80% ethanol and extract for 1 hour, add 0.03 times the amount of vitamin E of the Plantago seeds, reflux in a water bath at 60°C for 2 hours, filter, collect the filtrate, remove the ethanol by vacuum rotary evaporation at 45°C~60°C, filter, and obtain the Plantago seed extract.

[0051] Scheme K: Soak the plantago seed in an 8.0% arginine aqueous solution for 1 hour, wash, add 5 times the volume of 80% ethanol and extract for 1 hour, add 0.03 times the amount of vitamin E of the plantago seed, reflux in a water bath at 60°C for 2 hours, filter, collect the filtrate, remove the ethanol by vacuum rotary evaporation at 45°C~60°C, filter, and obtain the plantago seed extract.

[0052] Table 2 Contents of geniposide and verbascoside in the Plantago seed extracts of each regimen Table 2 shows the contents of geniposide and guanosine in the extracts of Plantago seeds from Scheme A to Scheme K. It can be seen that soaking Plantago seeds with glycine, tyrosine, threonine, serine, cysteine, and L-aspartic acid, and then extracting with ethanol, can effectively increase the amount of geniposide and guanosine extracted and enhance the efficacy of the drug. Studies have found that soaking Plantago seeds with hydrophilic amino acids can play the following roles: (1) Improve solubility: Hydrophilic amino acids can form hydrogen bonds or ionic bonds with polar active ingredients in Plantago seeds, increase the solubility of certain ingredients in the solvent, and thus increase the concentration of active ingredients in the extract. (2) Promote cell wall destruction: Amino acids can help destroy cell walls or cell membranes by interacting with polysaccharides and proteins in medicinal materials, thereby increasing the release rate of active ingredients. (3) Improve extraction efficiency: By increasing the solubility and release rate of active ingredients in medicinal materials, hydrophilic amino acids can improve the overall extraction efficiency and make the extraction process more efficient. (4) Enhanced stability: The combination of amino acids and active ingredients may help protect these ingredients, reduce degradation or deterioration during the extraction process, and enhance the stability of the extract. In addition, vitamin E has both hydrophilic and hydrophobic properties and is an amphiphilic substance. It can reduce the surface tension of the active ingredients in the medicinal materials, increase their solubility in the solvent, and make the active ingredients easier to release from the medicinal materials.

[0053] Optimization and screening of the mass fraction of amino acid aqueous solution To explore the selection range of the mass fraction of amino acid aqueous solution, we selected 1.0%, 2.0%, 3.0%, 4.0%, 5.0%, 6.0%, 7.0%, 8.0%, 9.0%, 10.0%, 12.0%, 15.0% glycine aqueous solution, 1.0%, 2.0%, 3.0%, 4.0%, 5.0%, 6.0%, 7.0%, 8.0%, 9.0%, 10.0%, 12.0%, 15.0% tyrosine aqueous solution, 1.0%, 2.0%, 3.0%, 4.0%, 5.0%, 6.0%, 7.0%, 8.0%, 9.0%, 10.0%, 12.0%, 15.0% threonine aqueous solution, and 1.0%, 2.0%, 3.0%, 4.0%, 5.0%, 6.0%, 7.0%, 8.0%, 9.0%, 10.0%, 12.0%, 15.0% threonine aqueous solution. .0%, 9.0%, 10.0%, 12.0%, 15.0% serine aqueous solution, 1.0%, 2.0%, 3.0%, 4.0%, 5.0%, 6.0%, 7.0%, 8.0%, 9.0%, 10.0%, 12.0%, 15.0% cysteine ​​aqueous solution, 1.0%, 2.0%, 3.0%, 4.0%, 5.0%, 6.0%, 7.0%, 8.0%, 9.0%, 10.0%, 12.0%, 15.0% L-aspartic acid aqueous solution were used to soak plantago seeds for 1 hour, washed, added with 5 times the volume of 80% ethanol for 1 hour, added with 0.03 times the amount of vitamin E of plantago seeds, refluxed in a water bath at 60℃ for 2 hours, filtered, collected the filtrate, and evaporated under reduced pressure at 45℃~60℃ to remove ethanol, filtered, to obtain plantago seed extract. The content of geniposide and verbascoside in plantago seed extracts was determined. It was found that the content of active ingredients extracted from plantago seed was higher when it was soaked in 5.0% to 10.0% aqueous solutions of glycine, tyrosine, threonine, serine, cysteine, and L-aspartic acid. Only the extraction effects of different mass fractions of glycine and threonine aqueous solutions are shown here ( Figure 1 、 Figure 2 ).

[0054] Example 1 Chinese medicine composition Formula: 200 grams of corn silk, 400 grams of dodder seed, 200 grams of oyster, 200 grams of cornus officinalis, 300 grams of euryale fruit, 200 grams of white poria, 100 grams of asparagus, 200 grams of lotus seeds, 100 grams of polygala root, 100 grams of ophiopogon, 100 grams of rehmannia root, 200 grams of schisandra chinensis, 50 grams of eucommia barbata, 150 grams of plantain seed, 100 grams of yam, 100 grams of wolfberry, 100 grams of codonopsis pilosula, 100 grams of magnolia vine leaves, 100 grams of cypress seed, 150 grams of raspberry, 400 grams of jujube (without kernel), 200 grams of ginseng, and 100 grams of salvia miltiorrhiza.

[0055] Preparation method: Step a: Grind ginseng and paper mulberry leaves into fine powder, pass through a 300-mesh sieve to obtain medicinal powder, and set aside; Step b: soaking the plantago seed in an 8.0% glycine aqueous solution for 1 hour, washing, adding 5 times the volume of 80% ethanol for 1 hour, adding 0.03 times the amount of vitamin E of the plantago seed, reflux extraction in a water bath at 60°C for 2 hours, filtering, collecting the filtrate, and removing the ethanol by vacuum rotary evaporation at 45°C-60°C, filtering, and obtaining the plantago seed extract; Step c: After processing corn silk, dodder seed, oyster, cornus officinalis, Euryale ferox, white poria, asparagus, lotus seed, polygala root, ophiopogon, prepared rehmannia root, schisandra chinensis, moringa officinalis, Chinese yam, wolfberry fruit, codonopsis pilosula, costus root, cypress seed, raspberry, jujube, and salvia miltiorrhiza, add 7 times water and boil for 1 hour, filter, and set aside the filtrate; add 5 times water to the filter residue and boil for 2 hours, filter, collect the filtrate, combine the two filtrates, and concentrate to a relative density of 1.35 to obtain an aqueous extract for set aside; Step d: Evenly mix the water extract, the plantago seed extract and the medicinal powder, add 85 grams of honey, 50 grams of starch and 15 grams of lactose, mix well and prepare pills.

[0056] Example 2 Chinese medicine composition Formula: 150 grams of corn silk, 350 grams of dodder seed, 180 grams of oyster, 170 grams of cornus officinalis, 250 grams of euryale fruit, 150 grams of white poria, 75 grams of asparagus, 180 grams of lotus seeds, 90 grams of polygala root, 80 grams of ophiopogon, 90 grams of rehmannia root, 50 grams of schisandra chinensis, 35 grams of eucommia barbata, 110 grams of plantain seed, 75 grams of yam, 70 grams of wolfberry, 80 grams of codonopsis pilosula, 50 grams of magnolia officinalis, 125 grams of mulberry leaves, 80 grams of cypress seed, 120 grams of raspberry, 350 grams of jujube (without kernel), 150 grams of ginseng, and 80 grams of salvia miltiorrhiza.

[0057] Preparation method: Step a: Grind ginseng and paper mulberry leaves into fine powder, pass through a 300-mesh sieve to obtain medicinal powder, and set aside; Step b: soaking the plantago seed in a 5.0% glycine aqueous solution for 0.5 h, washing, adding 3 times the volume of 75% ethanol to extract for 0.5 h, adding 0.01 times the amount of vitamin E of the plantago seed, reflux extraction in a water bath at 55° C. for 1.5 h, filtering, collecting the filtrate, and removing the ethanol by vacuum rotary evaporation at 45° C. to 60° C., filtering, and obtaining the plantago seed extract; Step c: After processing corn silk, dodder seed, oyster, cornus officinalis, Euryale ferox, white poria, asparagus, lotus seed, polygala root, ophiopogon, prepared rehmannia root, schisandra chinensis, moringa officinalis, Chinese yam, wolfberry, codonopsis pilosula, costus root, cypress seed, raspberry, jujube, and salvia miltiorrhiza, add 6 times water and boil for 0.5h, filter, and set aside the filtrate; add 4 times water to the filter residue and boil for 1.5h, filter, collect the filtrate, combine the two filtrates, and concentrate to a relative density of 1.25 to obtain an aqueous extract for later use; Step d: Evenly mix the water extract, the plantago seed extract, and the medicinal powder, add 45 grams of sucrose, 10 grams of fructose, 2 grams of sucralose, 20 grams of polyvinyl alcohol, and 75 grams of starch, mix well, and prepare pills.

[0058] Example 3 Chinese medicine composition Formula: 250 grams of corn silk, 450 grams of dodder seed, 220 grams of oyster, 230 grams of cornus officinalis, 350 grams of euryale fruit, 250 grams of white poria, 120 grams of asparagus, 240 grams of lotus seeds, 130 grams of polygala root, 130 grams of ophiopogon, 140 grams of rehmannia root, 150 grams of schisandra chinensis, 85 grams of eucommia barbata, 180 grams of plantain seed, 125 grams of yam, 150 grams of wolfberry, 125 grams of codonopsis pilosula, 110 grams of magnolia officinalis, 170 grams of mulberry leaves, 130 grams of cypress seed, 180 grams of raspberry, 430 grams of jujube (without kernel), 240 grams of ginseng, and 125 grams of salvia miltiorrhiza.

[0059] Preparation method: Step a: Grind ginseng and paper mulberry leaves into fine powder, pass through a 300-mesh sieve to obtain medicinal powder, and set aside; Step b: soaking the plantago seed in a 10.0% glycine aqueous solution for 1.5 hours, washing, adding 6 times the volume of 85% ethanol for 1.5 hours, adding 0.05 times the amount of vitamin E of the plantago seed, reflux extraction in a water bath at 65°C for 2.5 hours, filtering, collecting the filtrate, and removing the ethanol by vacuum rotary evaporation at 45°C-60°C, filtering, and obtaining the plantago seed extract; Step c: After processing corn silk, dodder seed, oyster, cornus officinalis, Euryale ferox, white poria, asparagus, lotus seed, polygala root, ophiopogon, prepared rehmannia root, schisandra chinensis, moringa officinalis, Chinese yam, wolfberry, codonopsis pilosula, costus root, cypress seed, raspberry, jujube, and salvia miltiorrhiza, add 8 times water and boil for 1.5 hours, filter, and set aside the filtrate; add 6 times water to the filter residue and boil for 2.5 hours, filter, collect the filtrate, combine the two filtrates, and concentrate to a relative density of 1.45 to obtain an aqueous extract for later use; Step d: Evenly mix the water extract, the plantago seed extract and the medicinal powder, add 115 grams of xylitol, 15 grams of mannitol and 25 grams of starch, mix well and prepare pills.

[0060] Anti-aging effect of the Chinese medicine composition of the present invention Telomeres are repetitive DNA sequences located at the ends of chromosomes. They protect chromosomes during cell division. Telomeres shorten with each cell division, which is considered a sign of cellular aging and an indicator of life expectancy. 18-month-old SD rats were randomly divided into four groups: Example 1, Example 2, Example 3, and a blank group, with 10 rats in each group. After two weeks of adaptive feeding, rats in Example 1, Example 2, and Example 3 groups were gavaged with the corresponding drug at a crude drug dose of 8 g / kg twice daily for two months. To quantify the relative telomere length in the rats' circulating blood, blood genomic DNA was extracted using the VAMNE MagUltra Blood Genomic DNA Extraction Kit (Nanjing Novozan Biotechnology Co., Ltd., DM101-01) and then mixed with SYBR Fast Mix (Takara, CN830A) and primers. Telomere length was assessed using the telomere length ratio for each group. The telomere length ratio (T / S ratio) is a quantitative measure of the ratio of telomeric repeat copies (T) to single-copy gene copies (S) in a sample. 36B4 was amplified as a single-copy gene. RT-qPCR was performed using an ABI 7500 real-time system. Each sample and primer combination was run in triplicate, and relative telomere length was calculated using the 2-∆∆Ct method.

[0061] Figure 3 The relative telomere length of rats in each group was significantly different after administration of the rats in Example 1, Example 2, and Example 3 groups compared with the rats in the blank group. P <0.01 (calculated using SPSS22.0).

[0062] The Chinese medicine composition of the present invention improves immunity 18 months old SD rats were randomly divided into 4 groups, namely Example 1 group, Example 2 group, Example 3 group, and blank group, with 10 rats in each group. After 2 weeks of adaptive feeding, the rats in Example 1 group, Example 2 group, and Example 3 group were gavaged with the corresponding drug, with a crude drug amount of 8g / kg, twice a day, for 2 months. The blank group was given normal saline. Rat serum was taken and the immunoglobulin (IgA, IgG, IgM) and cytokine (IL-2) content of each group of rats were detected according to the operating procedures in the ELISA kit instructions.

[0063] Table 3 Rat immunoglobulin (IgA, IgG, IgM) content Compared with the blank group, ** P <0.01.

[0064] The normal level and function of immunoglobulins are crucial to immunity. IgA can protect the mucosal surface and prevent pathogen invasion; IgG can fight bacteria and viruses and maintain long-term immune memory; and IgM can provide initial defense against new infections. The coordinated action of these immunoglobulins can effectively improve the body's overall immune capacity and help defend against various infections and diseases. The Chinese medicine compositions of Examples 1 to 3 of the present invention can significantly increase the levels of immunoglobulins (IgA, IgG, and IgM) in rats, playing a role in enhancing immune function. Compared with the blank group, there is a significant difference ( P <0.01).

[0065] IL-2 is secreted by activated T cells and is an important cytokine that regulates the immune system. It mainly promotes the proliferation and differentiation of T cells, enhances cell-mediated immune responses, and can also stimulate the activity of other immune cells (such as B cells and natural killer cells) to enhance the overall immune response. Figure 4 The expression of cytokines (IL-2) in the serum of rats in each group. The Chinese medicine compositions of Examples 1 to 3 of the present invention can significantly increase the expression of cytokines (IL-2) in the serum of rats, thereby improving the immune function. Compared with the blank group, there is a significant difference ( P <0.01).

[0066] The above data were statistically analyzed using SPSS 22.0 software, and the measurement data were analyzed using ( ) indicates that the comparison between multiple groups was performed by one-way analysis of variance, and the comparison between two groups was performed by independent sample T test. P The difference was statistically significant when the value was <0.05.

Claims

1. A Chinese medicine composition, characterized in that The traditional Chinese medicine composition consists of corn silk, dodder seed, oyster, cornus officinalis, gorgon fruit, white poria, asparagus, lotus seed, polygala root, ophiopogon, rehmannia root, schisandra chinensis, moringa officinalis, plantain seed, yam, wolfberry fruit, codonopsis pilosula, costus root, mulberry leaf, cypress seed, raspberry, jujube, ginseng and salvia miltiorrhiza.

2. The Chinese medicine composition according to claim 1, characterized in that The Chinese medicine composition is calculated by weight as follows: 150-250 parts of corn silk, 350-450 parts of dodder seeds, 180-220 parts of oysters, 170-230 parts of cornus officinalis, 250-350 parts of euryale seeds, 150-250 parts of white poria, 75-120 parts of asparagus, 180-240 parts of lotus seeds, 90-130 parts of polygala root, 80-130 parts of ophiopogon, 90-140 parts of prepared rehmannia root, 50-150 parts of schisandra chinensis, 35-85 parts of Morinda officinalis, 110-180 parts of Plantago seeds, 75-125 parts of Chinese yam, 70-150 parts of wolfberry fruits, 80-125 parts of Codonopsis pilosula, 50-110 parts of Radix Aucklandiae, 125-170 parts of Broussonetia mulberry leaves, 80-130 parts of Platycladi seeds, 120-180 parts of Rubus idaeus, 350-430 parts of jujube (without kernel), 150-240 parts of ginseng, and 80-125 parts of Salvia miltiorrhiza.

3. The Chinese medicine composition according to claim 1, characterized in that The Chinese medicine composition is calculated by weight as follows: 200 parts of corn silk, 400 parts of dodder seeds, 200 parts of oysters, 200 parts of cornus officinalis, 300 parts of euryale ferox, 200 parts of white tuckahoe, 100 parts of asparagus cochinchinensis, 200 parts of lotus seeds, 100 parts of polygala root, 100 parts of ophiopogon japonicus, 100 parts of rehmannia root, 200 parts of schisandra chinensis, 50 parts of moringa officinalis, 150 parts of plantain seeds, 100 parts of yam, 100 parts of wolfberry fruits, 100 parts of codonopsis pilosula, 100 parts of costus root, 150 parts of paper mulberry leaves, 100 parts of cypress seed, 150 parts of raspberry, 400 parts of jujube (without kernel), 200 parts of ginseng, and 100 parts of salvia miltiorrhiza.

4. A method for preparing the Chinese medicine composition according to claim 1, characterized in that: The preparation method is: Step a: Grind ginseng and paper mulberry leaves into fine powder, sieve to obtain medicinal powder, and set aside; Step b: soaking the plantago seed in an aqueous solution with a mass fraction of 5.0% to 10.0% amino acid for 0.5 to 1.5 hours, washing, adding 3 to 6 times the volume of 75% to 85% ethanol for 0.5 to 1.5 hours, adding 0.01 to 0.05 times the amount of vitamin E of the plantago seed, refluxing in a water bath at 55° C. to 65° C. for 1.5 to 2.5 hours, filtering, collecting the filtrate, and removing the ethanol by vacuum rotary evaporation at 45° C. to 60° C., filtering, and obtaining the plantago seed extract; Step c: after processing corn silk, dodder seed, oyster, cornus officinalis, euryale ferox, white poria, asparagus, lotus seed, polygala root, ophiopogon, prepared rehmannia root, schisandra chinensis, moringa officinalis, yam, wolfberry, codonopsis pilosula, costus root, cypress seed, raspberry, jujube, and salvia miltiorrhiza, add 6 to 8 times water and boil for 0.5 to 1.5 hours, filter, and set aside the filtrate; add 4 to 6 times water to the filter residue and boil for 1.5 to 2.5 hours, filter, collect the filtrate, combine the two filtrates, and concentrate to a relative density of 1.25 to 1.45 to obtain an aqueous extract for later use; Step d: uniformly mix the water extract, the plantago seed extract and the medicinal powder, add an appropriate amount of flavoring agent and / or filler, mix well, and prepare pills.

5. The method for preparing the Chinese medicine composition according to claim 4, wherein: The sieve in step a is a 300 mesh sieve.

6. The method for preparing the Chinese medicine composition according to claim 4, characterized in that: The amino acids are one or more of glycine, tyrosine, threonine, serine, cysteine, and L-aspartic acid.

7. The method for preparing the Chinese medicine composition according to claim 4, characterized in that: The flavoring agent is one or more of honey, sucrose, fructose, sucralose, aspartame, and xylitol.

8. The method for preparing the Chinese medicine composition according to claim 4, characterized in that: The filler is one or more of starch, microcrystalline cellulose, mannitol, lactose, and polyvinyl alcohol.

9. Use of the traditional Chinese medicine composition according to claim 1 in the preparation of anti-aging drugs.

10. Use of the traditional Chinese medicine composition according to claim 1 in the preparation of a drug for improving immunity.

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