Composition with multi-effect anti-aging effect and application thereof

Abstract

The invention relates to the technical field of daily chemicals, in particular to a composition with a multi-effect anti-aging effect and application of the composition. The composition is prepared from retinol, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, lactobacillus / soybean milk fermentation product filtrate and resveratrol. The invention relates to a composition with a triple anti-aging system capable of mildly promoting collagen regeneration and a preparation method of the composition, the composition comprises retinol, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, lactobacillus / soybean milk fermentation product filtrate and resveratrol, and the composition has an obvious effect on skin epidermis aging and dermis aging; the skin flora balance is regulated on the epidermis, the water locking capacity of the cuticle is enhanced, the permeability of active ingredients is improved, and the activity of cells is improved; in epidermis and dermis, the composition has anti-oxidation and anti-inflammatory characteristics, is mild in components and good in absorption effect, does not need to establish tolerance and consolidate skin resistance, and is particularly suitable for sensitive skin.

Description

Technical Field

[0001] The present invention relates to the technical field of daily chemicals, and in particular to a composition with multi-effect anti-aging effects and application thereof. Background Art

[0002] Skin aging refers to the deterioration of cellular structure and function in skin tissue due to the constant influence of internal and external factors. It typically manifests as dry skin, pigmentation spots, decreased skin elasticity, sagging, roughness, and wrinkles. In summary, skin aging can be divided into endogenous (20%) and exogenous (80%) causes. Endogenous aging refers to aging caused by irresistible physiological factors such as genetics and endocrine system, while exogenous aging is environmentally influenced by factors such as light radiation, environmental pollution, and unhealthy lifestyle habits. In addition to sun exposure, oxidative stress, non-enzymatic glycation, and genetic factors, the most significant causes of skin aging are a decrease in the number and function of fibroblasts, excessive collagen loss, and decreased mucopolysaccharide synthesis. These factors lead to a loosening of the dermal reticular structure, resulting in depressions and the appearance of wrinkles, sagging, dryness, and roughness on the skin's surface. Elastin enables the skin to stretch and fold, maintaining and supporting its elasticity and contributing to its resilience and suppleness. Collagen is a major component of the skin, comprising 72% of the skin, with 80% of the dermis comprising collagen. Therefore, collagen has the function of plumping the skin, rejuvenating it, and increasing its plumpness. Data shows that 53.2% of consumers begin to focus on anti-aging products between the ages of 26 and 35, while 23.2% begin to focus on anti-aging products between the ages of 18 and 25. With the advent of an aging society in China, the topic of "anti-aging" has become a hot topic among the public. Evolving lifestyles and cosmetic habits, coupled with a desire to maintain youthfulness and minimize the signs of aging, have directly driven the rapid growth of anti-aging product sales. The accelerated aging process provides enormous potential for development in my country's anti-aging market.

[0003] One of the core benefits of anti-aging products is to repair damaged cells and the skin's barrier function. However, single ingredients or low concentrations can only address a few specific aging issues, making a single product inadequate for comprehensive anti-aging needs. Stacking functional products can potentially burden the skin, push it to its limits, and lead to a rebound in short-term results upon discontinuation, all of which pose significant challenges for anti-aging users. According to a survey, 42.2% of anti-aging users reported not finding an anti-aging product suitable for their skin type or skin concerns. Furthermore, many products often contain emulsifiers or preservatives, which can disrupt the skin's ecological balance and lead to other skin problems over time. Many brands neglect the crucial step of cellular activation, resulting in a lack of skin vitality and poor absorption. Simply supplementing with collagen, without addressing multiple skin issues, is not only ineffective but can also clog pores due to poor absorption, accelerating aging. Summary of the Invention

[0004] To address these technical shortcomings, the present invention addresses the entire aging process. This approach addresses the evolving anti-aging landscape, which is undergoing a profound shift in consumer perceptions. Anti-aging ingredients place dual demands on consumers: They demand comprehensive efficacy to achieve multi-dimensional improvements, while also eagerly awaiting a sustainable, safe, and gentle anti-aging approach. This approach is driving the consumer market's shift from prioritizing efficacy to balancing efficacy and safety. The present composition addresses anti-aging from all perspectives, offering comprehensive, systematic solutions such as cell activation, collagen replenishment, and repair and soothing.

[0005] In this composition, retinol in the epidermis and dermis stimulates collagen regeneration and quickly improves wrinkles; in the dermis, palmitoyl tripeptide-1 continuously activates collagen synthesis, palmitoyl tetrapeptide-7 prevents collagen degradation through anti-inflammatory effects, resveratrol neutralizes free radicals, synergizes with retinol to reduce photosensitivity, inhibits the damage of MMP enzymes to collagen, and prolongs the duration of activity of other ingredients. The natural lactic acid in the fermentation product can gently metabolize keratin and reduce retinol stimulation. The peptide ingredients buffer the risk of retinol peeling, and the oxidation system protects Protect new collagen from oxidation and destruction; form a triple anti-aging closed loop of immediate repair + long-term promotion + prevention of loss; add lactobacillus / soy milk fermentation product filtrate to the epidermal microecology to regulate the balance of skin flora, enhance the stratum corneum's ability to lock water, and increase the penetration rate of active ingredients; ultimately build a new paradigm of "antioxidant-anti-inflammatory-promoting" complex anti-aging, which is particularly suitable for the comprehensive care needs of early-aging skin, photoaging skin and skin that stays up late, and provides users with a root cause, gentle, safe and long-lasting anti-aging solution for preventing aging. It surpasses traditional ingredients in terms of broad-spectrum antioxidant, collagen regeneration efficiency and anti-inflammatory depth, and promotes anti-aging skin care into a truly gentle, long-lasting, precise and efficient era.

[0006] To solve the above technical problems, the present invention provides a composition with multi-effect anti-aging effects, which comprises: retinol, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, lactobacillus / soy milk fermentation product filtrate, and resveratrol.

[0007] As an embodiment of the present invention, the retinol is encapsulated and modified retinol, and the resveratrol is liposome-encapsulated resveratrol.

[0008] As an embodiment of the present invention, the mass ratio of retinol, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, lactobacillus / soy milk fermentation product filtrate, and resveratrol is: (0.01-0.25): (0.0003-0.0008): (0.00015-0.0004): (0.05-0.2): (0.0001-0.03).

[0009] As an embodiment of the present invention, the mass ratio of retinol, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, lactobacillus / soy milk fermentation product filtrate, and resveratrol is: 0.2:0.0007:0.0004:0.2:0.003.

[0010] As an embodiment of the present invention, the preparation method of the encapsulated modified retinol is as follows:

[0011] Retinol was dissolved in an ethanol / propylene glycol mixed solvent at a concentration of 3 mg / mL; the volume ratio of ethanol / propylene glycol was 3:2;

[0012] Weigh 10 times the molar amount of β-cyclodextrin relative to retinol and 0.7 mol times the molar amount of stigmasterol relative to retinol, add deionized water at 45-55°C to form a suspension, and stir until uniform;

[0013] The organic phase dissolving retinol is slowly added dropwise to the suspension; the reaction is continued for 4 hours under magnetic stirring, the temperature is controlled below 50° C. in the dark, and then freeze-dried to obtain inclusion compound powder to encapsulate the modified retinol.

[0014] As an embodiment of the present invention, the preparation method of the liposome-encapsulated resveratrol is as follows:

[0015] S01 Weigh 2 g of lecithin, 0.8 g of cholesterol, 0.1 g of ceramide NP, and 0.3 g of phosphatidylserine, place in a glass beaker; add 10 mL of anhydrous ethanol until completely dissolved;

[0016] S02: Add 0.1 g of resveratrol to the mixture obtained in step S01, and continue stirring in a 60°C water bath to form a uniform transparent lipid phase;

[0017] S03: Mix glycerol and deionized water in a mass ratio of 2:96, maintaining the temperature at 55-60°C; then slowly add the transparent lipid phase dropwise to the aqueous phase while stirring at 800 rpm to maintain uniform emulsification; after the addition is complete, continue stirring for 15-20 minutes.

[0018] As an embodiment of the present invention, the composition with multiple anti-aging effects is applied in the field of daily chemicals.

[0019] An essence, characterized in that its preparation raw materials include the composition with multiple anti-aging effects.

[0020] As an embodiment of the present invention, the raw materials for preparing the essence include: water, pentylene glycol, hexylene glycol, glycerin, allantoin, disodium EDTA, trehalose, xanthan gum, sodium polyglutamate, β-glucan, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, squalane, tocopheryl acetate, caprylic / capric triglyceride, polyacrylate-13 polyglyceryl-10 stearate, C14-22 alcohol / C12-20 alkyl glucoside, phosphatidylcholine, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, retinol, resveratrol, and lactobacillus / soy milk fermentation product filtrate.

[0021] By adopting the above technical solution, the present invention has the following beneficial effects:

[0022] Retinol: New microencapsulation technology and high encapsulation content make the product more stable and gentle in sustained-release retinol. Retinol can increase the gene expression of elastin and fibrillin-1 in normal human dermal fibroblasts (NHDF), dermal 3D culture models and human skin explants. It is one of the most effective collagen protectors and collagen promoters, including promoting elastin synthesis, promoting procollagen synthesis and elastic fiber network formation, promoting type I and type III collagen synthesis, and increasing the gene expression of tropoelastin / fibrillin-1 / type I collagen. In addition, it can increase the synthesis of hyaluronic acid and effectively counteract the activity of MMPs, thereby preventing signs of photoaging, increasing skin firmness, plumpness and smoothness, increasing epidermal thickness, and slowing down the aging symptoms caused by the loss of these two complexes, thereby preventing fine lines and wrinkles that have not yet formed. Retinol is a mild exfoliant that regulates the shedding of keratinocytes in the stratum corneum. It also softens the keratin and moisturizes the skin, thereby improving skin radiance and moisturization. Retinol promotes cell proliferation and stimulates cell renewal, thus thickening the epidermis and repairing and improving damage caused by chronological aging and photoaging. Since wound healing requires the migration and proliferation of keratinocytes, retinol can promote skin repair and regeneration.

[0023] The polypeptide sequence of Palmitoyl Tripeptide-1 plays an important feedback regulatory role in connective tissue reconstruction and cell proliferation. By increasing fibroblast activity, it can promote the production of large amounts of skin repair proteins during the skin repair process. It has the benefits of promoting collagen and mucopolysaccharide synthesis, moisturizing, firming the skin, and reducing wrinkles.

[0024] Palmitoyl Tetrapeptide-7: There is a strong correlation between the decline of DHEA and the increase of IL-6 during aging. DHEA can control the level of inflammatory cytokines in the circulatory system, while Palmitoyl Tetrapeptide-7 has the functions of regulating inflammatory factors (IL-6), mimicking DHEA (youth hormone), and maintaining skin immune homeostasis, thereby reducing the damage of various inflammatory factors to skin tissue. By acting on keratinocytes and fibroblasts to inhibit the release of inflammatory factor IL-6, it reduces the damage of inflammation to the skin, tightens the skin, and reduces wrinkles. It is known as the "youth hormone peptide."

[0025] Lactobacillus / Soymilk Ferment Filtrate: Lactobacillus / Soymilk Ferment Filtrate works synergistically with highly nutritious black beans fermented in a human biomimetic manner to significantly modulate the skin's microbiome. In vitro testing has shown that this ingredient can effectively enhance the diversity of beneficial skin bacteria, increase the abundance of Staphylococcus epidermidis, maintain a healthy, slightly acidic pH environment, reduce the risk of allergies, rapidly relieve pain caused by external stimuli, improve inflammatory responses, and instantly increase skin hydration for long-lasting, deep moisturization. Furthermore, it soothes and repairs the skin from multiple dimensions, helping to maintain skin stability and health, meeting consumers' evolving skin needs. In vitro experiments have further confirmed that this ingredient can promote collagen synthesis in fibroblasts, accelerate hyaluronic acid production, enhance keratinocyte regeneration, and improve the healing and repair efficiency of damaged tissue.

[0026] Resveratrol: Resveratrol is a non-flavonoid phenolic substance, a phytoalexin and antioxidant belonging to the stilbene family of compounds. The benefits of resveratrol on the skin have garnered increasing research attention in recent years, and its mechanisms of action in skincare are now largely understood. Resveratrol exhibits significant whitening and photoprotection properties, enhancing protection against UV rays, boosting the body's antioxidant capacity, and reducing inflammatory responses. Furthermore, resveratrol can improve skin's keratinocyte proliferation, promote keratinocyte differentiation, improve the skin's ability to repair damage, and reduce scar tissue formation. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the specific embodiments or the description of the prior art. Obviously, the drawings described below are some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative work.

[0028] Figure 1 This is a performance test diagram of the essence prepared in Example 2. DETAILED DESCRIPTION

[0029] The present invention will be further explained below with reference to specific embodiments.

[0030] The composition of the present invention is made into an anti-aging essence, and the formula is as shown in Table 1:

[0031]

[0032]

[0033]

[0034]

[0035] In the above table, the preparation method of lipid resveratrol is as follows:

[0036] (1) 2 g of lecithin, 0.4 g of stigmasterol, and 0.1 g of resveratrol were mixed and dissolved in an ethanol:propylene glycol (4:1) system and stirred at 60°C until a transparent phase formed.

[0037] (2) Using pH 6.0 PBS buffer as the aqueous phase, the lipid phase was added dropwise after heating to 60°C, and then high-shear homogenization was performed at 8000 rpm for 5 minutes, followed by membrane extrusion to obtain liposomes with uniform particle size; finally, freeze-dried liposome powder was obtained by freeze drying.

[0038] In the above table, the preparation method of the essence is as follows:

[0039] Group A: Water, Pentylene Glycol, Hexylene Glycol, Glycerin, Disodium EDTA, Trehalose, Xanthan Gum, Sodium Polyglutamate, β-Glucan, Hydroxyethyl Acrylate / Sodium Acryloyldimethyl Taurate Copolymer;

[0040] Group B: Squalane, Caprylic / Capric Triglyceride, Tocopheryl Acetate, Polyglyceryl-10 Stearate, C14-22 Alcohol / C12-20 Alkyl Glucoside, Phosphatidylcholine;

[0041] Group C: Palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, retinol, resveratrol, lactobacillus / soy milk fermentation product filtrate, allantoin;

[0042] Phase S01 A: Preparation of aqueous phase: Heat purified water to 75±2°C, add glycerol, pentanediol, hexylene glycol, trehalose, and disodium EDTA in sequence, and stir until completely dissolved; disperse xanthan gum, which can be pre-wetted with a small amount of glycerol before adding, and stir until no flocculation occurs; add sodium polyglutamate, β-glucan, and hydroxyethyl acrylate / sodium acryloyldimethyltaurate copolymer in sequence, stir evenly, and maintain the temperature at 75°C for later use;

[0043] Phase S02 B: Heat and dissolve the oil phase: Place squalane, triglycerides, and tocopheryl acetate in an oil phase beaker and heat to 75±2°C; add polyglyceryl-10 stearate and C14-22 alcohol / C12-20 alkyl glucoside and stir thoroughly until completely dissolved; finally, add phosphatidylcholine and continue stirring until a uniform and clear state is achieved;

[0044] S03: Slowly add the oil phase (B) to the water phase (A), and shear emulsify at 8000 rpm in an emulsifier for 5 to 10 minutes; then switch to a stirrer, maintain the temperature at about 70°C, and continue stirring for 10 minutes to form colostrum;

[0045] Slowly cool the S04 emulsified system to below 40°C, add the remaining ingredients while stirring at a low speed to avoid bubbles.

[0046] illustrate:

[0047] 1. Comparative Example 1 is an essence obtained by removing palmitoyl tripeptide-1 from the essence of Example 2 of the present invention.

[0048] 2. Comparative Example 2 is an essence obtained by removing palmitoyl tetrapeptide-7 from the essence of Example 2 of the present invention.

[0049] 3. Comparative Example 3 is an essence obtained by removing retinol from the essence of Example 2 of the present invention.

[0050] 4. Comparative Example 4 is an essence obtained by removing resveratrol from the essence of Example 2 of the present invention.

[0051] 5. Comparative Example 5 is an essence obtained by removing the lactobacillus / soy milk fermentation product filtrate from the essence of Example 2 of the present invention.

[0052] 6. Comparative Example 6 is an essence obtained by replacing the retinol in the essence of Example 2 of the present invention with commercially available unencapsulated retinol.

[0053] 7. Comparative Example 7 is an essence obtained by removing polyglyceryl-10 stearate, C14-22 alcohol, C12-20 alkyl glucoside, and phosphatidylcholine from the essence of Example 2 of the present invention.

[0054] 8. Comparative Example 8 is an essence obtained by replacing the resveratrol in the essence of Example 2 of the present invention with commercially available lipid-free resveratrol.

[0055] 9. Comparative Example 9 is an essence obtained by replacing the resveratrol in the essence of Example 2 of the present invention with resveratrol in the form of other commercially available lipids.

[0056] Performance Testing

[0057] (1) Efficacy test

[0058] 1. Test instruments:

[0059] 1. This invention uses the Finnish Delfin ElastiMeter (skin elasticity meter). The ElastiMeter uses an indenter to gently press against the skin. When an external force is applied to the skin, the skin resists changes in shape. The skin's response under short-term load indicates its immediate elastic properties, thereby obtaining the skin's elasticity value.

[0060] 2. Photographs were taken using the Finnish Delfin Antera 3D. Antera3D uses innovative optical methods and complex mathematical algorithms to acquire three-dimensional images. This allows data to be extracted from the images regarding the skin's three-dimensional shape, enabling the quantification of treatment effects and monitoring of changes over time. For example, skin texture and smoothness, as well as the depth and width of fine lines and wrinkles, can be measured. Using its multispectral analysis, the Antera software can also measure pigmentation and redness, providing meaningful information on sun damage, vein lines, and more.

[0061] 3. The Delfin VapoMeter SWL5201 transepidermal water loss meter from Finland was used. The VapoMeter has a cylindrical chamber containing relative humidity and temperature sensors. Shortly after the instrument is placed in contact with the skin, the relative humidity (RH%) in the measurement chamber increases linearly. The SCH value is calculated based on the increase in RH%. Between measurements, the measurement chamber is passively ventilated. The VapoMeter uses a closed-chamber measurement principle, so measurements are not affected by room airflow. The short measurement time ensures that interruption of normal evaporation is minimized.

[0062] 2. Experimental Principle:

[0063] SCH value, transepidermal water loss (SCH) measures the change in water vapor pressure near the skin surface. The SCH value indicates the loss of water from the stratum corneum and is an important criterion for evaluating the barrier function of the stratum corneum. The lower the SCH value of the skin, the better the barrier function of the skin, and vice versa. By testing the SCH value of skin water loss, the efficacy of moisturizing and repairing cosmetics can be evaluated. It can also be used in allergic patch tests, contact dermatitis, physical therapy, burns and monitoring of new tissue to promptly detect whether the protective function of the skin has been destroyed. The unit of SCH is: g / hm 3 .

[0064] 3. Test population:

[0065] There are 20 people in each group, for a total of 11 groups, including volunteers aged 30-65 who meet the experimental requirements.

[0066] 4. Test conditions:

[0067] At room temperature of 21±1°C and relative humidity of 50±10%, real-time dynamic monitoring is performed and the subjects sit quietly for 30 minutes before testing the relevant data.

[0068] 5. Experimental process:

[0069] This experiment was divided into 11 groups. Group 1 used Example 1 of the present invention on the entire face, Group 2 used Example 3 of the present invention on the entire face, Group 3 used Comparative Example 7 on the entire face, Groups 4-11 used Example 2 of the present invention on the left face, and each group applied Comparative Examples 1-6 and 8-9 on the right face. The product was applied to the face twice a day, morning and evening, for 4 weeks. The skin elasticity (ISE) value, transepidermal water loss (SCH) value, average wrinkle length (WRC) (mm), average wrinkle width (WRK) (mm), and average wrinkle depth (WRS) (mm) were recorded. Then, each volunteer's face, one part each of the left and right foreheads, canthi of the eyes, and cheeks, was photographed using Antera 3D and archived.

[0070] 6. Experimental results:

[0071] 1. Skin stratum corneum water content (SCH value) test

[0072] In this test, the SCH measured by the subject in week 0 is set as SCH0, and the SCH in week 4 is set as SCH4. The SCH change value is △SCH, △SCH = SCH4-SCH0, △SCH change rate = (SCH4-SCH0) / SCH0*100%. The △SCH change rate is a positive number, and the higher the value, the more moisture the skin has after four weeks of using the product, which means the skin is more hydrated and plump, and the better the effect of the product in increasing the moisture content of the skin.

[0073] Table 2 shows the average ΔSCH change rate of 20 people in each group after 4 weeks of use compared with the initial value, as follows:

[0074] Table 2 Change rate of skin stratum corneum water content (SCH)

[0075]

[0076]

[0077] 2. Skin elasticity (ISE) test

[0078] In this test, the ISE value measured at week 0 is set as ISE0, and the ISE value at week 4 is set as ISE4. The ISE change value is △ISE, △ISE = ISE4 - ISE0, and the △ISE change rate = (ISE4 - ISE0) / ISE0 * 100%. The △ISE change rate is a positive number, and the higher the value, the greater the increase in skin elasticity after four weeks of use, the higher the skin elasticity, and the skin becomes firmer, plumper, and more elastic. Table 3 shows the average △ISE change rate value of 20 people in each group compared with the initial value after four weeks of use, as follows:

[0079] Table 3 Change rate of skin elasticity (ISE)

[0080] Group △ISE change rate (%) Example 1 24.31 Example 2 29.061 Example 3 33.53 Comparative Example 1 17.65 Comparative Example 2 16.74 Comparative Example 3 15.43 Comparative Example 4 19.86 Comparative Example 5 12.32 Comparative Example 6 10.27 Comparative Example 7 8.94 Comparative Example 8 11.85 Comparative Example 9 13.67

[0081] Skin wrinkle length (WRC value), average wrinkle width (WRK), average wrinkle depth (WRS) test: This test sets the average wrinkle length, width, and depth measured by the subjects in week 0 as WRC0, WRK0, and WRS0, and the average wrinkle length, width, and depth measured in week 4 as WRC4, WRK4, and WRS4. The wrinkle change values ​​are △WRC, △WRK, and △WRS, △WRC=WRC4-WRC0, △WRK=WRK4-WRK0, △WRS=WR S4-WRS0; △WRC change rate = (WRC4-WRC0) / WRC0*100%, △WRK change rate = (WRK4-WRK0) / WRK0*100%, △WRS change rate = (WRS4-WRS0) / WRS0*100%; △WRC, △WRK, and △WRS change rates are negative numbers, and lower values ​​indicate that wrinkles are becoming shorter, narrower, and shallower. The product's ability to delay wrinkle formation is better, resulting in smoother, more youthful-looking skin. Table 4 shows the average change rates of wrinkle length, width, and depth for each group of 20 people after four weeks of use, compared to the initial values.

[0082] Table 4 Change rate of wrinkle length (WRC), width (WRK), and depth (WRS)

[0083]

[0084]

[0085] (2) Safety test (human skin patch test)

[0086] 1. Number of testers: 40 people in total, 20 males and 20 females, aged between 18 and 60.

[0087] 2. Health status: The subject has healthy skin, no history of skin allergies, and meets the voluntary inclusion criteria.

[0088] 3. Test Method: Use a qualified patch tester and place approximately 0.020-0.2025 g of the test product in the closed patch tester. Apply the test product to the tester's back with special adhesive tape. Each tester should have 11 application points. Remove the test product after 24 hours. Observe the skin reaction 0.5, 24, and 48 hours after removal. Score the skin reaction grading standard in accordance with the "Hygienic Standards for Cosmetics" and calculate the average value. The results are recorded in Table 6.

[0089] Table 5 Human skin patch test standard table

[0090]

[0091] Table 6 Skin patch test scoring results

[0092]

[0093]

[0094] Stability test (stability test of the composition of the present invention on the final product)

[0095] 1. Experimental instruments: 101-1AB electric blast drying oven (constant temperature 50 degrees), QC609 biological ultraviolet yellowing resistance tester

[0096] 2. Experimental process: The essences prepared in Examples 1 to Comparative Example 9 were placed in 100 ml containers and placed in a drying oven and a yellowing resistance test respectively. The test results are shown in Tables 7 and 8.

[0097] Table 7 Heat resistance constant temperature 50 degrees test

[0098]

[0099] Table 8 Ultraviolet irradiation test

[0100]

[0101]

[0102] Select some volunteers' facial wrinkle images before and after using the embodiment of the present invention to compare, see Figure 1 As can be seen intuitively from the figure, after using the essence prepared in Example 2 of the present invention on the face, the overall level of facial wrinkles is reduced, indicating that the anti-wrinkle effect of the composition of the present invention is very excellent.

[0103] The present invention discloses a triple anti-aging system that gently promotes collagen regeneration and its preparation method. The composition comprises retinol, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, lactobacillus / soy milk fermentation product filtrate, and resveratrol. The composition significantly reduces aging in both the epidermis and dermis. In the epidermis, it regulates the balance of skin flora, enhances the stratum corneum's ability to retain moisture, increases the penetration rate of active ingredients, and increases cell vitality. In the epidermis and dermis, it exhibits antioxidant and anti-inflammatory properties. Its mild composition is well absorbed, eliminating the need for tolerance building and strengthening skin's resistance, making it particularly suitable for sensitive skin. In the dermis, it increases the production of collagen, elastin, and mucopolysaccharides, improving skin elasticity and reducing wrinkles, achieving a comprehensive anti-aging effect. The effectiveness of the composition has been demonstrated through extensive experiments, and each component is irreplaceable. The optimal range of addition for the composition is also achieved, achieving an optimal range for both effectiveness and cost. In summary, the composition of the present invention is a sustained and effective triple anti-aging system that gently promotes collagen regeneration.

[0104] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit it. Although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the above embodiments, or replace some or all of the technical features therein with equivalents. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention.

Claims

1. A composition with multiple anti-aging effects, characterized in that: The composition comprises retinol, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, lactobacillus / soy milk fermentation product filtrate, and resveratrol.

2. A composition with multiple anti-aging effects according to claim 1, characterized in that: The retinol is modified retinol, and the resveratrol is liposome-encapsulated resveratrol.

3. A composition with multiple anti-aging effects according to claim 2, characterized in that: The mass ratios of retinol, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, lactobacillus / soy milk fermentation product filtrate, and resveratrol are: (0.01-0.25): (0.0003-0.0008): (0.00015-0.0004): (0.05-0.2): (0.0001-0.03).

4. The composition with multiple anti-aging effects according to claim 2, characterized in that: The mass ratio of retinol, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, lactobacillus / soy milk fermentation product filtrate, and resveratrol is: 0.2:0.0007:0.0004:0.2:0.

003.

5. The composition with multiple anti-aging effects according to claim 2, characterized in that: The preparation method of the encapsulated modified retinol is as follows: Retinol was dissolved in an ethanol / propylene glycol mixed solvent at a concentration of 3 mg / mL; the volume ratio of ethanol / propylene glycol was 3:2; Weigh 10 times the molar amount of β-cyclodextrin relative to retinol and 0.7 mol times the molar amount of stigmasterol relative to retinol, add deionized water at 45-55°C to form a suspension, and stir until uniform; The organic phase dissolving retinol is slowly added dropwise to the suspension; the reaction is continued for 4 hours under magnetic stirring, the temperature is controlled below 50° C. in the dark, and then freeze-dried to obtain inclusion compound powder to encapsulate the modified retinol.

6. The composition with multiple anti-aging effects according to claim 2, characterized in that: The preparation method of the liposome-containing resveratrol is as follows: S01 Weigh 2 g of lecithin, 0.8 g of cholesterol, 0.1 g of ceramide NP, and 0.3 g of phosphatidylserine, place in a glass beaker; add 10 mL of anhydrous ethanol until completely dissolved; S02: Add 0.1 g of resveratrol to the mixture obtained in step S01, and continue stirring in a 60°C water bath to form a uniform transparent lipid phase; S03: Mix glycerol and deionized water in a mass ratio of 2:96, maintaining the temperature at 55-60°C; then slowly add the transparent lipid phase dropwise to the aqueous phase while stirring at 800 rpm to maintain uniform emulsification; after the addition is complete, continue stirring for 15-20 minutes.

7. The composition with multiple anti-aging effects according to any one of claims 1 to 6, characterized in that: The composition is applied in the field of daily chemicals.

8. An essence, characterized in that: The raw materials for its preparation include the composition with multiple anti-aging effects as claimed in any one of claims 1 to 6.

9. The essence according to claim 8, characterized in that Its preparation raw materials include: water, pentylene glycol, hexylene glycol, glycerin, allantoin, disodium EDTA, trehalose, xanthan gum, sodium polyglutamate, β-glucan, hydroxyethyl acrylate / sodium acryloyldimethyl taurate copolymer, squalane, tocopheryl acetate, caprylic / capric triglyceride, polyacrylate-13 polyglyceryl-10 stearate, C14-22 alcohol / C12-20 alkyl glucoside, phosphatidylcholine, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, retinol, resveratrol, lactobacillus / soy milk fermentation product filtrate.

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