Pomacea canaliculata oocyst incubation inhibitor, water body pomacea canaliculata and oocyst comprehensive control method thereof

Abstract

The invention provides a pomacea canaliculata oocyst incubation inhibitor, a water body pomacea canaliculata and an oocyst comprehensive prevention and treatment method thereof.The pomacea canaliculata oocyst incubation inhibitor is prepared from, by weight, 0.2-0.5 part of azadirachtin, 1-2 parts of guanidine hydrochloride, 1-2 parts of trypsin, 0.05-0.2 part of polyether modified heptamethyltrisiloxane, 1-2 parts of barite powder and 100 parts of water. The comprehensive prevention and treatment method comprises the steps that comprehensive prevention and treatment of the water body pomacea canaliculata and the oocysts of the pomacea canaliculata are conducted in the mode that the pomacea canaliculata killing reagent and the pomacea canaliculata oocyst incubation inhibitor are combined, the pomacea canaliculata killing reagent rapidly kills pomacea canaliculata larvae and adults, and the pomacea canaliculata oocyst incubation inhibitor inhibits pomacea canaliculata While larvae and adults of the pomacea canaliculata are killed, incubation of newborn oocysts is inhibited synchronously, incubation of the newborn oocysts is blocked to supplement populations, and breeding and spreading of the pomacea canaliculata are controlled through a comprehensive prevention and control system.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of the prevention and control of the invasion of the apple snail species, and in particular to an apple snail egg capsule hatching inhibitor and a comprehensive prevention and control method for apple snails and their egg capsules in water bodies. BACKGROUND

[0002] The apple snail belongs to the phylum of mollusca, the class of gastropods, and the genus of ampullariidae. It prefers to live in freshwater bodies such as rivers, ponds, ditches and various paddy fields with fertile soil and aquatic plants, and likes to eat higher plants and benthic organisms. It is originally from the Amazon River Basin in South America and has parasitic angusticaudex. Since it was introduced into China as a food snail in 1981, it has caused great harm and disaster, and was listed as one of the world's 100 most harmful invasive alien species in 2000.

[0003] The apple snail egg capsule is a bright red long strip or oval hard capsule containing 100-400 embryos, which is usually attached to aquatic plants, rocks or other hard surfaces and takes about 2 weeks to develop into a juvenile and then released into the water. The newly born egg capsule is soft and elastic, and is adhered together by the transparent mucous secreted by the mother snail. The mucous contains protein components but no polysaccharides. The egg capsule is bright red and has a warning color, contains neurotoxins and anti-protein hydrolase, and has a strong defense mechanism, making the apple snail eggs almost have no predators. The photos of the apple snail and its egg capsule in the water are shown in Figure 1 .

[0004] For the prevention and control of the apple snail, the principle of "prevention first and comprehensive control" is mainly adopted. In the apple snail heavy area, the use of ecological environment safe and efficient molluscicides is the current mainstream. Molluscicides mainly include chemical synthetic drugs and plant component drugs. The chemical synthetic drugs mainly include niclosamide, fentin acetate, emamectin benzoate, sodium pentachlorophenol, carbaryl and tetraacetal, etc. These drugs have good molluscicidal effect, but have problems such as great toxicity to non-target organisms, easy to have negative impact on the environment, etc. The plant source molluscicides have the characteristics of environmental friendliness and have become a new research starting point for the prevention and control of the apple snail, such as tea saponin, bloodroot alkaline sulfate, etc., but most of them have the problem of slow action. In addition, most of the chemical and plant drugs will have adverse effects on aquatic organisms such as fish, and when the same reagent is used for a long time, the apple snail is easy to develop resistance.

[0005] Current studies on apple snails mainly focus on adult control and have achieved remarkable results. Given the strong reproductive capacity of apple snails, a female snail can produce 30,000 to 50,000 eggs per year, and it is difficult to completely control the spread of apple snails by controlling the number of adult snails alone. After control, the snail population often rebounds rapidly. As an important stage in the life history of apple snails, egg capsules can be effectively controlled by inhibiting their hatching. However, current egg capsule control mainly relies on manual removal, which is time-consuming and labor-intensive (1-2 times per week), especially for large areas of water where egg capsules are difficult to collect. Therefore, studying methods to inhibit the hatching of apple snail egg capsules is of great significance in reducing the frequency of manual egg removal and the frequency of adding snail killing reagents, and in controlling the spread of apple snails.

[0006] To address the problem of rebounding in the control of apple snails due to the isolated implementation of adult snail killing and egg capsule inhibition, a comprehensive control method is urgently needed to achieve long-term and effective control of apple snails. SUMMARY

[0007] To solve the problems in the background art, the present application provides a kind of apple snail egg capsule hatching inhibitor, water body apple snail and its egg capsule comprehensive control method. The apple snail egg capsule hatching inhibitor can effectively inhibit the hatching of apple snail egg capsules, and based on this, a water body apple snail and its egg capsule comprehensive control method is provided, which simultaneously inhibits the hatching of newly hatched egg capsules while killing apple snail larvae and adults, blocks the hatching of newly hatched egg capsules to replenish the population, controls the spread of apple snails through a comprehensive control system, and achieves long-term and effective control of apple snails. The technical solution of the present application to solve the above technical problems is as follows: In the first aspect, the present application provides a kind of apple snail egg capsule hatching inhibitor, which is composed of the following components by weight: azadirachtin 0.2-0.5 parts, guanidine hydrochloride 1-2 parts, trypsin 1-2 parts, polyether modified heptamethyltrisiloxane 0.05-0.2 parts, barite powder 1-2 parts, and water 100 parts.

[0008] The apple snail egg capsule hatching inhibitor adsorbs on the mucus on the surface of the egg capsule, forming a layer of closed hard shell-like structure, destroying the new egg surface layer mucus, reducing the egg capsule resistance, and at the same time destroying the egg red protein and lipid carotenoid protein structure of the egg capsule, inhibiting the normal hatching of the embryo. Polyether modified trisiloxane as a penetration enhancer promotes the penetration of effective ingredients into the inner layer of the egg capsule.

[0009] The apple snail egg capsule inhibitor of the present application has good hatching inhibition effect on fresh red egg capsules produced within 0-5 days, can make the egg capsules not hatch at all, partially hatch or hatch for a longer time, and the indoor hatching inhibition rate of the apple snail egg capsule inhibitor on the newly produced egg capsules within 0-5 days can reach up to 90%.

[0010] In a second aspect, the application provides a method for using the above-mentioned inhibiting agent for hatching of apple snail egg capsules, which comprises spraying the inhibiting agent for hatching of apple snail egg capsules on the newly laid apple snail egg capsules within 0-5 days.

[0011] According to the above scheme, the spraying is performed once a day, and the spraying is performed for 1-3 days.

[0012] By artificially spraying the inhibiting agent for hatching of apple snail egg capsules, the hatching of the egg capsules is inhibited, and the problem of difficulty in manually removing the eggs and shortage of manpower is solved, and compared with the manual removal method, time and labor are saved, and the efficiency is high.

[0013] In a third aspect, the application provides a comprehensive prevention and control method for apple snails and their egg capsules in water bodies, which comprises combining an apple snail killing agent with the above-mentioned inhibiting agent for hatching of apple snail egg capsules to comprehensively prevent and control the apple snails and their egg capsules in water bodies, wherein the apple snail killing agent rapidly kills the apple snail larvae and adults, and the inhibiting agent for hatching of apple snail egg capsules inhibits the hatching of the apple snail egg capsules.

[0014] From the perspective of eradicating apple snails, the apple snails are killed while the influence of continuous hatching of the egg capsules on rapid recovery of the population is fully considered, and a comprehensive prevention and control method combining the apple snail killing agent for killing the apple snails with the inhibiting agent for hatching of apple snail egg capsules for inhibiting the hatching of the egg capsules is proposed, so that the adults and larvae of the apple snails are killed while the hatching of the newly born egg capsules is inhibited synchronously, the population replenished by the hatching of the newly born egg capsules is blocked, the propagation and spread of the apple snails are controlled through the comprehensive prevention and control system, the recurrence of the apple snails is avoided, and the prevention and control effect is improved. Moreover, the artificial workload can be greatly reduced, time and labor are saved, and the prevention and control efficiency is improved.

[0015] According to the above scheme, the comprehensive prevention and control method for the apple snails and their egg capsules in water bodies comprises the following steps: S1. Killing of apple snail adults and larvae: applying the apple snail killing agent in the water area where the apple snails are in an outbreak state, applying the apple snail killing agent once every 2-4 weeks, and continuously applying the apple snail killing agent for 2-5 cycles, so that most of the adults are killed in the operation site, and most of the egg capsules are hatched and fall into the water to be killed in the cycle; S2. Inhibition of hatching of apple snail egg capsules: after the apple snail killing agent is continuously applied for 2-3 cycles, the inhibiting agent for hatching of apple snail egg capsules is sprayed regularly, and the manually removing of the eggs and the killing of the apple snails after hatching are supplemented.

[0016] The present application is based on the growth cycle of apple snails and the incubation cycle of egg capsules, and proposes to kill the adult apple snails in 2-4 weeks as a cycle, until most of the adult apple snails and the hatched young are killed, and then stop dosing, and then cooperate with the apple snail egg capsule hatching inhibitor, and manually clean the eggs and kill the apple snails after hatching, through the cooperation of multiple means and the setting of the use period of the reagent, the control effect of the apple snails is guaranteed, the recurrence and spread of the apple snails are avoided, the use frequency of the apple snail killing reagent and the egg capsule hatching inhibitor is reduced, and the adverse effects on the environment and aquatic organisms are reduced.

[0017] According to the above scheme, wetland aquatic plants are harvested in the fourth quarter to reduce the attachment of apple snail egg laying areas.

[0018] According to the above scheme, in step S2, the apple snail egg capsule hatching inhibitor is sprayed every 3-6 weeks as a cycle, and the apple snail supplementary killing and manual egg cleaning are carried out every 1-2 months until the adult apple snails and egg capsules disappear.

[0019] According to the above scheme, in step S2, the apple snail egg capsule hatching inhibitor is sprayed in the egg capsule production 0-5 days, once a day, for 1-3 days, on the aquatic plants, the shore wall or other apple snail egg laying places; or bamboo or other long strips are inserted in the water to attract apple snails to climb on the bamboo or other long strips and concentrate egg laying, and the apple snail egg capsule hatching inhibitor is sprayed.

[0020] According to the above scheme, the apple snail killing reagent is at least one of tetraacetaldehyde and sophoramine.

[0021] According to the above scheme, when the tetraacetaldehyde and sophoramine are used as the apple snail killing reagent respectively, the field applicable concentrations are 2-4 mg / L and 2-4 mg / L respectively, and when the tetraacetaldehyde and sophoramine are used as the apple snail killing reagent jointly, the applicable concentrations are 1-2 mg / L and 1-2 mg / L respectively.

[0022] The present application fully considers the influence of chemical method on the ecosystem of water body (such as wetland and paddy field), and optimizes two apple snail killing reagents, chemical reagent tetraacetaldehyde and plant reagent sophoramine, which can achieve 100% lethality rate of apple snails, have significant control effect, can be used for killing adult and young apple snails in the outbreak period, have low toxicity to fish in the recommended use range, and the sophoramine can be naturally decomposed in the natural environment, the product is carbon dioxide and water, has the characteristics of environmental friendliness, and the two reagents are suitable for emergency use in water bodies such as wetlands and paddy fields.

[0023] The present application has the following beneficial effects: 1) The apple snail ovicapsule inhibitor of the present application can inhibit the hatching of apple snail ovicapsules, and the indoor inhibition rate of the ovicapsules of the newly hatched apple snails within 0-5 days can reach up to 90%, spraying is used to inhibit the hatching of apple snail ovicapsules, reduces the frequency of artificial egg cleaning, saves manpower and material resources, and improves the efficiency of apple snail ovicapsule control; 2) The present application proposes a comprehensive control method combining the killing of apple snails by the apple snail killing agent and the inhibition of ovicapsule hatching by the apple snail ovicapsule hatching inhibitor, which synchronously inhibits the hatching of newly born ovicapsules while killing apple snail larvae and adults, breaks the population replenishment of newly born ovicapsules, controls the propagation and spread through the comprehensive control system, avoids the problem of re-occurrence and spread after the killing of apple snails alone, further, according to the growth characteristics and reproduction cycle of apple snails, an optimal dosing cycle combination scheme is proposed, which achieves the best control effect while reducing the workload of the staff and the amount of drug during the control process, saves manpower and material resources, improves the control efficiency, and avoids pollution to the environment. BRIEF DESCRIPTION OF DRAWINGS

[0024] Figure 1 The photos of apple snails and their ovicapsules in the background art of the present application; Figure 2 The indoor test photos of the apple snail ovicapsule hatching inhibitor of the present application; Figure 3 The indoor killing result photos of the apple snail killing agent of the present application; Figure 4 The toxicity photos of the apple snail killing agent of the present application to fish; Figure 5 The field killing effect photos of the apple snail killing agent of the present application; Figure 6 The comprehensive control effect photos of the apple snails of the present application. DETAILED DESCRIPTION

[0025] The principles and characteristics of the present application are described below in combination with the drawings and specific examples, and the examples are only used to explain the present application and are not used to limit the scope of the present application.

[0026] Example 1: Apple snail ovicapsule hatching inhibitor Take 5g azadirachtin, 20g guanidine hydrochloride, 20g trypsin, 2g polyether modified heptamethyltrisiloxane, and 20g barite powder, add 1L water and mix to prepare apple snail ovicapsule hatching inhibitor I; Take 2g azadirachtin, 10g guanidine hydrochloride, 10g trypsin, 0.5g polyether modified heptamethyltrisiloxane, and 10g barite powder, add 1L water and mix to prepare apple snail ovicapsule hatching inhibitor II; Take 3g azadirachtin, 15g guanidine hydrochloride, 15g trypsin, 1g polyether modified heptamethyl trisiloxane, 15g barite powder, add 1L water and mix to make the golden apple snail egg capsule hatching inhibitor III.

[0027] (1) Laboratory test of the golden apple snail egg capsule hatching inhibitor The above prepared golden apple snail egg capsule hatching inhibitor is used for the laboratory test of the golden apple snail egg capsule hatching inhibition. The specific method is as follows: put the postpartum 0~5 days, size and color basically consistent egg capsules into a plastic box, immerse the egg capsules in the treatment liquid for 20 seconds, and place them in the natural temperature condition for hatching. The hatching inhibition rate is calculated 10 days after the first egg capsule hatches. The azadirachtin, guanidine hydrochloride, trypsin, polyether modified trisiloxane and barite powder are used as controls respectively. The results are shown in Table 1 and Figure 2 .

[0028] Table 1 Inhibition effect of the laboratory test of the golden apple snail egg capsule hatching inhibitor on the egg capsule hatching

[0029] From the above table, it can be seen that the golden apple snail egg capsule hatching inhibitor has good inhibition effect on the hatching of fresh red egg capsules produced within 0~5 days. It can make the egg capsules not hatch at all, partially hatch or prolong the hatching time. The highest laboratory hatching inhibition rate of the new egg capsules produced within 0~5 days can reach 90%.

[0030] (2) Field inhibition test of the golden apple snail egg capsule hatching inhibitor The above prepared golden apple snail egg capsule hatching inhibitor is used for the field test of the golden apple snail egg capsule hatching inhibition. The specific method is as follows: In wetland test water area 1 with an area of 500m 2 and an average water depth of 40cm, after the golden apple snails are killed, the golden apple snail egg capsule hatching inhibitor I is sprayed for 3 consecutive days. The hatching of 20 egg capsules in the selected area is observed regularly. The results show that when the prepared golden apple snail egg capsule hatching inhibitor I is used for the egg capsules in the field, the egg capsules are found to hatch on the 20th day. After 30 days of application of the golden apple snail egg capsule hatching inhibitor I, the number of hatched egg capsules is 3, and the hatching inhibition rate is 85%.

[0031] In wetland test water area 1 with an area of 500m 2 and an average water depth of 40cm, after the golden apple snails are killed, the golden apple snail egg capsule hatching inhibitor I is sprayed for 2 consecutive days. The hatching of 20 egg capsules in the selected area is observed regularly. The results show that when the prepared golden apple snail egg capsule hatching inhibitor I is used for the egg capsules in the field, the egg capsules are found to hatch on the 17th day. After 30 days of application of the golden apple snail egg capsule hatching inhibitor I, the number of hatched egg capsules is 4, and the hatching inhibition rate is 80%.

[0032] In wetland test water area 1 with an area of 500m 2, the average water depth is 40 cm, after the killing of apple snails, spray 1 time of apple snail egg capsule hatching inhibitor I, regularly observe 20 egg capsule hatching conditions in selected area, the results show that the prepared apple snail egg capsule hatching inhibitor I is used for egg capsule in the field, the egg capsule hatches on the 14th day, after 30 days of application of apple snail egg capsule hatching inhibitor I, the hatching number is 7, and the hatching inhibition rate is 65%.

[0033] The water area of wetland test 2 is 300 m 2 , the average water depth is 40 cm, after the killing of apple snails, spray 1 time of apple snail egg capsule hatching inhibitor I, regularly observe 20 egg capsule hatching conditions in selected area, the results show that the prepared apple snail egg capsule hatching inhibitor I is used for egg capsule in the field, the egg capsule hatches on the 14th day, after 30 days of application of apple snail egg capsule hatching inhibitor I, the hatching number is 7, and the hatching inhibition rate is 65%.

[0034] Example 2 Preparation of apple snail killing reagent Three groups of apple snail killing reagents are prepared, which are apple snail killing reagent I, apple snail killing reagent II and apple snail killing reagent III.

[0035] The apple snail killing reagent I is a water solution of tetrameric acetaldehyde, and the effective mass concentration of tetrameric acetaldehyde is 2 mg / L. The preparation method is as follows: 10 mg of tetrameric acetaldehyde is weighed and mixed in 5 L of water.

[0036] The apple snail killing reagent II is a water solution of tetrameric acetaldehyde, and the effective mass concentration of tetrameric acetaldehyde is 4 mg / L. The preparation method is as follows: 20 mg of tetrameric acetaldehyde is weighed and mixed in 5 L of water.

[0037] The apple snail killing reagent III is a mixed water solution of tetrameric acetaldehyde and sophoramine, the effective mass concentration of tetrameric acetaldehyde is 1 mg / L, and the effective mass concentration of sophoramine is 2 mg / L. The preparation method is as follows: 5 mg of tetrameric acetaldehyde and 10 mg of sophoramine are weighed and mixed in 5 L of water.

[0038] The apple snail killing reagent I, the apple snail killing reagent II and the apple snail killing reagent III prepared in this example are respectively subjected to adult apple snail indoor killing effect test, field killing effect test and fish toxicity test.

[0039] (1) Apple snail adult indoor killing test The apple snail adult indoor killing effect test method is as follows: 10 adult apple snails are added in a plastic box, and after 24 h, 48 h and 72 h of killing by using the apple snail killing reagent, water body recovery method is used to identify the death and life of apple snails, and the number of dead apple snails is recorded. The results are shown in Table 2 and Figure 3 .

[0040] Table 2 Indoor killing results of apple snail killing reagent

[0041] (2) Fish toxicity test of the apple snail killing agent The fish toxicity test of the apple snail killing agent: 20 fish fry of 9-12 cm in length were added into a plastic box, and after being soaked with the apple snail killing agent for 24, 48 and 72 hours, the number of dead fish fry was recorded, and the results are shown in Table 3 and Figure 4 .

[0042] Table 3 Toxicity of the apple snail killing agent to fish

[0043] (3) Field killing test of the apple snail killing agent The field killing effect test method of the apple snail killing agent is as follows: Select wetland test water area 1 with an area of about 500 m 2 , an average water depth of 40 cm, and add 800 g of effective component of tetraacetylaldehyde, and put 3 bags containing 20 apple snails in the wetland; after 1, 2 and 3 days of application, take out one bag per day to identify the death situation; Select wetland test water area 1 with an area of about 500 m 2 , an average water depth of 40 cm, and add 400 g of effective component of tetraacetylaldehyde, and put 3 bags containing 20 apple snails in the wetland; after 1, 2 and 3 days of application, take out one bag per day to identify the death situation; Select wetland test water area 2 with an area of about 300 m 2 , an average water depth of 40 cm, and add 120 g and 240 g of effective components of tetraacetylaldehyde and matrine, and put 3 bags containing 20 apple snails in the wetland; after 1, 2 and 3 days of application, take out one bag per day to identify the death situation, and the results are shown in Table 4 and Figure 5 .

[0044] Table 4 Field killing effect of the apple snail killing agent

[0045] Example 3 Comprehensive prevention and control of apple snails and their egg capsules in water bodies The prevention and control method is suitable for different habitats such as various water bodies (including but not limited to rice fields, irrigation ditches, natural wetlands and slow-flowing rivers) according to the wide adaptability of apple snails.

[0046] Take test water area 1 as an example to comprehensively prevent and control apple snails and their egg capsules.

[0047] Test water area 1 is a water area densely planted with Typha angustifolia, with an area of about 500 m 2, the average water depth is 40 cm, the fine leaf cattail is harvested in October, and the area for attaching and laying eggs of the apple snail is reduced; from December to March of the next year, the apple snail killing reagent II is added once a month in clear weather, and the operation is carried out for 4 times, the effective component dosage is 400 g each time, the dead snail residues floating on the water surface are fished up 3 days after each time, and it is found that the existing adult snails and juvenile snails in the water body are basically all killed; from April to October, the aquatic plants are replaced by the calla lily, the calla lily grows, and the apple snail enters a rapid breeding stage, and it is found that the apple snails start to lay eggs, the apple snail hatching inhibition reagent III is sprayed once a month, 3 days are needed after each spraying, and the unhatched egg capsules attached to the bank wall and the aquatic plants are manually picked up every 3 months; it is found that a small part of the egg capsules are hatched and fall into the water to develop during the operation and maintenance of the wetland, the apple snail is supplemented and killed once every 2 months, the killing reagent is the apple snail killing reagent II, and it is found on site that the density of the apple snails in the water is ≤0.2 / m 2 , no new egg capsules are found on the bank wall, and the specific control effect is shown in Figure 6 .

[0048] The above merely describes preferred embodiments of the present application and is not intended to limit the present application, and any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A hatching inhibitor for golden apple snail egg sacs, characterized in that, It is composed of the following components in parts by weight: 0.2-0.5 parts azadirachtin, 1-2 parts guanidine hydrochloride, 1-2 parts trypsin, 0.05-0.2 parts polyether-modified heptamethyltrisiloxane, 1-2 parts barite powder, and 100 parts water.

2. The method of using the golden apple snail egg sac hatching inhibitor as described in claim 1, characterized in that, Spray the newly laid golden apple snail egg sacs (0-5 days old) with the described golden apple snail egg sac hatching inhibitor.

3. A comprehensive control method for Pomacea canaliculata and its egg sacs in aquatic bodies, characterized in that, A comprehensive control method for the prevention and control of golden apple snails and their egg sacs in water is adopted by combining a golden apple snail killing agent with the golden apple snail egg sac hatching inhibitor described in claim 1. The golden apple snail killing agent rapidly kills golden apple snail larvae and adults, and the golden apple snail egg sac hatching inhibitor inhibits the hatching of golden apple snail egg sacs.

4. The integrated control method for Pomacea canaliculata and its egg sacs in aquatic bodies according to claim 3, characterized in that, Includes the following steps: S1. Killing of adult and juvenile golden apple snails: Apply golden apple snail killing agent to the waters in the golden apple snail outbreak area, with a cycle of 2 to 4 weeks. Apply the golden apple snail killing agent once in each cycle, and continue for 2 to 5 cycles. S2. Inhibition of golden apple snail egg sac hatching: After applying the golden apple snail killing agent for 2-3 consecutive cycles, spray the golden apple snail egg sac hatching inhibitor regularly, and supplement with artificial egg removal and additional killing of golden apple snails after hatching.

5. The integrated control method for Pomacea canaliculata and its egg sacs in aquatic bodies according to claim 4, characterized in that, Harvesting of wetland aquatic plants will be carried out in the fourth quarter to reduce the attachment of golden apple snails to their spawning areas.

6. The integrated control method for Pomacea canaliculata and its egg sacs in aquatic bodies according to claim 4, characterized in that, In step S2, a round of golden apple snail egg sac hatching inhibitor is sprayed every 3 to 6 weeks, and supplementary killing of golden apple snails and artificial egg removal are carried out every 1 to 2 months until the adult golden apple snails and egg sacs are basically gone.

7. The integrated control method for Pomacea canaliculata and its egg sacs in aquatic bodies according to claim 6, characterized in that, In step S2, during the spraying cycle of the golden apple snail egg sac hatching inhibitor, the golden apple snail egg sac hatching inhibitor is sprayed once a day for 1 to 3 consecutive days within 0 to 5 days after the egg sacs are laid. The spraying is applied to aquatic plants, bank walls, or other places where golden apple snails lay eggs; or bamboo strips or other long strips are inserted into the water to attract golden apple snails to climb onto the bamboo strips or other long strips and concentrate on laying eggs, and then the golden apple snail egg sac hatching inhibitor is sprayed.

8. The integrated control method for Pomacea canaliculata and its egg sacs in aquatic bodies according to any one of claims 3 to 7, characterized in that, The golden apple snail extermination agent is at least one of metaldehyde and matrine.

9. The integrated control method for Pomacea canaliculata and its egg sacs in aquatic bodies according to claim 8, characterized in that, When metaldehyde and matrine are used as insecticides for golden apple snails, their applicable field concentrations are 2-4 mg / L and 2-4 mg / L, respectively. When metaldehyde and matrine are used together as insecticides for golden apple snails, their applicable concentrations are 1-2 mg / L and 1-2 mg / L, respectively.

Images