Artificial breeding method for paraphyllous hemsleyanus

Abstract

The invention relates to the technical field of fish breeding. The invention provides an artificial breeding method for paraphyllous hemsleyanus, which comprises the following steps: (1) selecting parent paraphyllous hemsleyanus which is healthy, free of injury and disease, good in gonad development and more than 18 months old, breeding male and female parent fishes in different ponds, and controlling the ratio of male fishes to female fishes to be 1: (2-3); (2) selectively injecting a first oxytocic into the mature male fish, and injecting a second oxytocic into the sexually mature female fish to obtain induced spawning parent fish; (3) collecting seminal fluid of the induced spawning male fish and fish egg cells of the induced spawning female fish, carrying out artificial insemination, and hatching to obtain fry. By adopting the breeding method, generation of parent fish egg cells or sperms can be effectively promoted, so that the quality of fertilized eggs and the breeding success rate are improved; by utilizing the compound spawning induction substance, the spawning induction effect is enhanced, meanwhile, the use of chemical substances can be reduced, and drug residues and environmental pollution are reduced. The method is of great significance to artificial breeding and large-scale breeding of the paralifola speculatus.

Description

Technical Field

[0001] This invention relates to the field of fish breeding technology, and in particular to a method for the artificial breeding of the spotted cichlid. Background Technology

[0002] The Managuensis variegata, also known as the freshwater grouper, tiger tilapia, or jaguar cichlid, is a fish with high economic and ornamental value. With increasing market demand and dwindling wild populations, artificial breeding has become crucial for ensuring its supply. However, existing breeding techniques for Managuensis variegata have several problems. For example, natural reproduction is inefficient and cannot meet the demands of large-scale aquaculture; traditional artificial breeding methods cause significant damage to parent fish, resulting in low fertilization and fry survival rates, severely hindering the development of Managuensis variegata aquaculture. Therefore, there is an urgent need to develop an efficient and scientific artificial breeding method for Managuensis variegata. Summary of the Invention

[0003] The purpose of this invention is to provide a method for artificial breeding of the spotted cichlid. The method described in this invention can promote the production of parent fish eggs or sperm, improve the quality of fish eggs or sperm, and thus improve the quality of fertilized eggs and the seedling rate. While enhancing the spawning effect, it can also reduce the use of chemical substances, reduce drug residues and environmental pollution.

[0004] To achieve the above-mentioned objectives, the present invention provides the following technical solution:

[0005] This invention provides a method for artificially breeding flower cichlids, comprising the following steps:

[0006] (1) Select healthy, disease-free, and well-developed gonadal broodstock of 18 months or older, and raise male and female broodstock in separate ponds with a male-to-female ratio of 1:(2-3).

[0007] (2) Selectively mature male fish were injected with spawning induced agent 1, and sexually mature female fish were injected with spawning induced agent 2 to obtain spawning broodstock.

[0008] (3) Collect the semen of the induced male fish and the egg cells of the induced female fish and perform artificial insemination. After hatching, the fish fry are obtained.

[0009] Preferably, during the separate rearing of male and female broodstock in step (1), functional feed is added until 5-7 days before spawning; the daily amount of the functional feed is 0.08-0.2% of the broodstock's body weight.

[0010] Preferably, the functional excipients include 10-15 parts of Epimedium, 10-15 parts of Lycium barbarum, 5-8 parts of Astragalus membranaceus, 0.1-0.3 parts of Vitamin C, and 0.1-0.3 parts of Vitamin E.

[0011] Preferably, the oxytocin 1 comprises a traditional Chinese medicine composition A and dioxone in a dosage ratio of (2-4) mL:(1-3) mg.

[0012] Preferably, the traditional Chinese medicine composition A comprises the following components in parts by weight: 1-3 parts of Epimedium, 1-3 parts of Cuscuta chinensis, and 8-12 parts of Lycium barbarum.

[0013] Preferably, the oxytocin 2 is composed of a traditional Chinese medicine composition B comprising a dosage ratio of (3-6) mL:(2-4) mg:(500-800) IU, dioxone, and human chorionic gonadotropin.

[0014] Preferably, the traditional Chinese medicine composition B comprises the following components in parts by weight: 1-2 parts Angelica sinensis, 2-3 parts Ligustrum lucidum, 3-5 parts Rehmannia glutinosa, and 5-10 parts Leonurus japonicus.

[0015] Preferably, the broodstock induced in step (2) are cultured in a pool with a water temperature of 26-29℃, a salinity of 19-23‰, and a dissolved oxygen content of not less than 6mg / L.

[0016] Preferably, the volume ratio of the male fish semen to the female fish egg cells in step (3) is 1:(50-100).

[0017] By adopting the above technical solution, the present invention has the following beneficial effects:

[0018] 1. The addition of functional excipients in the technical solution of this invention can promote the maturation of the gonads of parent fish, promote the production of parent fish egg cells or sperm, and improve the quality of sperm or fish egg cells.

[0019] 2. The technical solution of this invention uses a combination of traditional Chinese medicine ingredients and compound preparations to induce spawning. The extracts of the traditional Chinese medicine ingredients regulate the endocrine system of broodstock, promote gonadal development and ovulation, and work synergistically with the compound preparations to more gently and effectively induce estrus, ovulation, and sperm release in broodstock, reducing stress on the broodstock, improving the spawning induction effect, and making the spawning time and quality of the broodstock more controllable. At the same time, the addition of traditional Chinese medicine ingredients reduces the use of chemical substances, reducing drug residues and environmental pollution.

[0020] 3. The technical solution of this invention uses Chinese medicinal materials as spawning-inducing additives to promote the secretion and expulsion of semen from male ichthyophthirius multifiliis, thereby ensuring subsequent artificial insemination.

[0021] 4. This invention reduces damage to parent fish during the egg collection, sperm collection, and fertilization processes, while ensuring thorough mixing of fish egg cells and sperm, thus significantly improving the fertilization rate.

[0022] 5. The breeding method described in this invention lays a solid foundation for subsequent breeding work, ensuring that the parent fish have good reproductive performance and improving the breeding success rate. This is of great significance for the artificial breeding and large-scale farming of the spotted cichlid. Detailed Implementation

[0023] This invention provides a method for artificially breeding flower cichlids, comprising the following steps:

[0024] (1) Select healthy, disease-free, and well-developed gonadal broodstock of 18 months or older, and raise male and female broodstock in separate ponds with a male-to-female ratio of 1:(2-3).

[0025] (2) Selectively mature male fish were injected with spawning induced agent 1, and sexually mature female fish were injected with spawning induced agent 2 to obtain spawning broodstock.

[0026] (3) Collect the semen of the induced male fish and the egg cells of the induced female fish and perform artificial insemination. After hatching, the fish fry are obtained.

[0027] In this invention, healthy, disease-free, and well-developed gonadal broodstock of the spotted cichlid are selected. The criteria for health are preferably bright red gills, the absence of mucus proliferation or parasites; the criteria for being disease-free are preferably the absence of ulcers, parasites, bleeding points on the body surface, intact scales, and undamaged fins. The male spotted cichlids of this invention are preferably over 24 months old and weigh ≥800g; the female spotted cichlids are preferably over 18 months old and weigh ≥500g.

[0028] In this invention, the male and female parent fish are cultured in separate ponds. The water temperature in the culture ponds is controlled at 25-28℃, the salinity is maintained at 18-22‰, and the dissolved oxygen content is not less than 5mg / L. The ratio of male to female fish is preferably 1:(2-3), more preferably 1:(2.2-2.8), and even more preferably 1:2.5.

[0029] In this invention, functional excipients are added during the separate rearing of male and female broodstock in separate ponds until 5-7 days before spawning induction. The functional excipients preferably include 10-15 parts Epimedium, 10-15 parts Lycium barbarum, 5-8 parts Astragalus membranaceus, 0.1-0.3 parts Vitamin C, and 0.1-0.3 parts Vitamin E; more preferably, they include 12-14 parts Epimedium, 11-14 parts Lycium barbarum, 6-7.5 parts Astragalus membranaceus, 0.15-0.25 parts Vitamin C, and 0.15-0.25 parts Vitamin E; and even more preferably, they include 13.5 parts Epimedium, 12 parts Lycium barbarum, 6.5 parts Astragalus membranaceus, 0.2 parts Vitamin C, and 0.2 parts Vitamin E.

[0030] In this invention, the Epimedium is rich in the active ingredient icariin, which promotes testosterone synthesis and increases spermatogonial cell division activity. The Lycium barbarum promotes the maturation of female fish gonads, increases the content of n-3 series unsaturated fatty acids (such as DHA), and reduces estradiol levels in the blood. These changes help improve egg quality and increase reproductive success rate; increase sperm density and motility, and reduce the rate of sperm abnormalities; it can also enhance the antioxidant capacity and immunity of fish, promoting growth and development. The Astragalus membranaceus can improve the immunity of aquatic animals, enhance their disease resistance, and also promote growth and development. The Vitamin C can promote collagen synthesis and prevent oocyte rupture; the Vitamin E can effectively protect the integrity of the sperm membrane and reduce lipid oxidative damage. In this invention, Epimedium is used in combination with Astragalus membranaceus, Lycium barbarum, etc., and further combined with Vitamin C and Vitamin E to promote an increase in gonadal hormone levels, further optimizing reproductive performance.

[0031] In this invention, the daily feeding amount of the functional feed is preferably 0.08-0.2% of the body weight of the parent fish, more preferably 0.1-0.18%, and even more preferably 0.12%.

[0032] In this invention, mature male cichlids are selectively injected with spawning induced agent 1, and sexually mature female cichlids are injected with spawning induced agent 2, to obtain spawning-induced broodstock.

[0033] In this invention, the oxytocin 1 comprises a traditional Chinese medicine composition A and domperidone (DOM), preferably obtained by mixing an extract of traditional Chinese medicine composition A with domperidone. The preferred dosage ratio of traditional Chinese medicine composition A to domperidone is (2-4) mL:(1-3) mg, more preferably (2.5-3.5) mL:(1.2-2.5) mg, and even more preferably 3 mL:1.8 mg. The traditional Chinese medicine composition A preferably comprises the following components in parts by weight: 1-3 parts Epimedium, 1-3 parts Cuscuta, and 8-12 parts Lycium; more preferably 1.5-2.8 parts Epimedium, 1.5-2.8 parts Cuscuta, and 9-11 parts Lycium; and even more preferably 2.2 parts Epimedium, 2.5 parts Cuscuta, and 10 parts Lycium.

[0034] In this invention, the preferred method of injecting per kilogram of male ichthyophthirius multifiliis is to inject oxytocin 1, which consists of 2-4 mL of extract of traditional Chinese medicine composition A and 1-3 mg of dioxin, and more preferably 3 mL of extract of traditional Chinese medicine composition A and 1.8 mg of dioxin.

[0035] In this invention, the oxytocin 2 comprises a traditional Chinese medicine composition B, dioxin, and human chorionic gonadotropin (HCG), preferably obtained by mixing an extract of traditional Chinese medicine composition B, dioxin, and HCG. The preferred ratio of the traditional Chinese medicine composition B, dioxin, and HCG is (3-6) mL:(2-4) mg:(500-800) IU, further preferably (3.5-5.5) mL:(2.2-3.8) mg:(550-750) IU, and even more preferably 5 mL:3 mg:650 IU. The preferred traditional Chinese medicine composition B comprises the following components in parts by weight: 1-2 parts Angelica sinensis, 2-3 parts Ligustrum lucidum, 3-5 parts Rehmannia glutinosa, and 5-10 parts Leonurus japonicus; more preferably, 1.2-1.8 parts Angelica sinensis, 2.2-2.8 parts Ligustrum lucidum, 3.5-4.5 parts Rehmannia glutinosa, and 6-9 parts Leonurus japonicus; even more preferably, 1.5 parts Angelica sinensis, 2.5 parts Ligustrum lucidum, 4 parts Rehmannia glutinosa, and 8 parts Leonurus japonicus.

[0036] In this invention, the preferred method of injecting per kilogram of female *Cyprinus fasciatus* is to inject oxytocin 2, which consists of 3-6 mL of extract of traditional Chinese medicine composition B, 2-4 mg of dioxone, and 500-800 IU of human chorionic gonadotropin. More preferably, it consists of 5 mL of extract of traditional Chinese medicine composition B, 3 mg of dioxone, and 650 IU of human chorionic gonadotropin.

[0037] In this invention, broodstock induced to spawn are cultured in a pool with a water temperature of 26-29℃, a salinity of 19-23‰, and a dissolved oxygen content of not less than 6mg / L.

[0038] In this invention, broodstock are observed for spawning. When the male's genital pore protrudes in a conical shape, with a noticeable bulge between the anus and genital pore; and the female's genital pore is round or oval, slightly red and protruding outwards, with a full, soft abdomen and loose skin around the vent, sperm and egg cells are collected. The preferred collection method is to use the pads of the right thumb and forefinger, starting from the posterior margin of the pectoral fin, gently sliding backwards along both sides of the abdominal midline to 1 cm in front of the vent, applying continuous, even, and gradually increasing pressure. The pressure should be limited to ensure smooth flow of eggs or sperm, avoiding explosive force.

[0039] In this invention, a dry method is used to artificially fertilize the collected semen and fish egg cells. The volume ratio of the male fish semen to the female fish egg cells is preferably 1:(50-100), more preferably 1:(60-90), and even more preferably 1:80.

[0040] In this invention, the fertilized eggs are preferably placed in an incubation device for incubation to obtain fish fry. The incubation water temperature is preferably 20-25℃, more preferably 21-23℃; the incubation time is preferably 3-8 days, more preferably 3-4 days; and the dissolved oxygen in the incubation water is preferably ≥5.0 mg / L, more preferably ≥7.0 mg / L.

[0041] The technical solutions provided by the present invention will be described in detail below with reference to the embodiments, but they should not be construed as limiting the scope of protection of the present invention.

[0042] Example 1

[0043] A method for artificially breeding flower-bodied cichlids, the steps of which are as follows:

[0044] (1) Select healthy, disease-free, and well-developed broodstock of the cichlid, with males being 24 months or older and weighing ≥800g, and females being 18 months or older and weighing ≥500g. The ratio of males to females should be 1:2.5.

[0045] (2) Separate male and female broodstock into different ponds for rearing. The water temperature in the rearing ponds is controlled at 28℃, the salinity is maintained at 22‰, and the dissolved oxygen content is not less than 5mg / L. During the rearing process of male and female broodstock in separate ponds, functional feed is added until 5 days before spawning. The daily feeding amount of functional feed is 0.12% of the body weight of the broodstock. The functional feed consists of 13.5 parts Epimedium, 12 parts Lycium barbarum, 6.5 parts Astragalus membranaceus, 0.2 parts Vitamin C, and 0.2 parts Vitamin E.

[0046] (3) Take 2.2 parts of Epimedium, 2.5 parts of Cuscuta and 10 parts of Lycium barbarum, add 6 times the volume of water to the herbal composition A, then decoct twice, each time for 45 minutes, combine the two filtrates, and concentrate at 60℃ to 1 / 10 of the original volume to obtain the extract of herbal composition A; mix the extract of herbal composition A with dioxin at a ratio of 3 mL: 1.8 mg to obtain oxytocin 1;

[0047] (4) Take 1.5 parts Angelica sinensis, 2.5 parts Ligustrum lucidum, 4 parts Rehmannia glutinosa and 8 parts Leonurus japonicus, add 8 times the volume of water to the herbal composition B, then decoct twice, each time for 50 minutes, combine the two filtrates, and concentrate at 60℃ to 1 / 10 of the original volume to obtain the herbal composition B extract; mix the herbal composition B extract, dioxin and human chorionic gonadotropin in a ratio of 5mL:3mg:650IU to obtain oxytocin 2;

[0048] (5) Selectively mature male fish were injected with oxytocin 1, and sexually mature female fish were injected with oxytocin 2 to obtain oxytocin-induced broodstock. The oxytocin-induced broodstock were cultured in a pond with a water temperature of 29℃, a salinity of 20‰, and a dissolved oxygen content of not less than 6mg / L. Each kilogram of male cichlids was injected with oxytocin 1, which consisted of 3mL of extract of Chinese medicine composition A and 1.8mg of dioxin. Each kilogram of female cichlids was injected with oxytocin 2, which consisted of 5mL of extract of Chinese medicine composition B, 3mg of dioxin, and 650IU of human chorionic gonadotropin.

[0049] (6) When the male fish’s genital opening protrudes into a cone shape and there is a noticeable bulge between the anus and the genital opening, and the female fish’s genital opening is round or oval, slightly red and slightly protruding, the abdomen is full and soft and the skin around the vent is loose, the sperm and fish egg cells are collected. Using the pads of the right thumb and index finger, start from the posterior edge of the pectoral fin and gently slide along both sides of the abdominal midline to 1 cm in front of the vent. Apply continuous, even and gradually increasing pressure, with the pressure limited to the smooth flow of eggs or sperm.

[0050] (7) Artificial insemination was performed on the collected semen and fish egg cells using a dry method at a volume ratio of 1:80;

[0051] (8) The obtained fertilized eggs were placed in an incubation device and incubated for 3 days in an environment with a water temperature of 23℃ and dissolved oxygen ≥7.0mg / L, and then cultured to obtain fish fry.

[0052] Example 2

[0053] A method for artificially breeding flower-bodied cichlids, the steps of which are as follows:

[0054] (1) Select healthy, disease-free, and well-developed broodstock of the cichlid, with males being 24 months or older and weighing ≥800g, and females being 18 months or older and weighing ≥500g. The ratio of males to females should be 1:2.5.

[0055] (2) Separate male and female broodstock into separate ponds for rearing. The water temperature in the rearing ponds is controlled at 28℃, the salinity is maintained at 22‰, and the dissolved oxygen content is not less than 5mg / L. During the rearing process of male and female broodstock in separate ponds, functional feed is added until 5 days before spawning. The daily feeding amount of functional feed is 0.2% of the body weight of the broodstock. The functional feed consists of 10 parts Epimedium, 15 parts Lycium barbarum, 5 parts Astragalus membranaceus, 0.1 parts Vitamin C, and 0.3 parts Vitamin E.

[0056] (3) Take 1 part of Epimedium, 3 parts of Cuscuta and 12 parts of Lycium barbarum, add 6 times the volume of water to the herbal composition A, then decoct twice, each time for 50 minutes, combine the two filtrates, and concentrate at 60℃ to 1 / 10 of the original volume to obtain the extract of herbal composition A; mix the extract of herbal composition A with dioxin at a ratio of 4 mL: 1 mg to obtain oxytocin 1;

[0057] (4) Take the Chinese herbal composition B containing 2 parts Angelica sinensis, 2 parts Ligustrum lucidum, 5 parts Rehmannia glutinosa and 6 parts Leonurus japonicus, add 7 times the volume of water, and then decoct twice, each time for 50 minutes. Combine the two filtrates and concentrate them to 1 / 10 of the original volume at 60℃ to obtain the extract of Chinese herbal composition B; mix the extract of Chinese herbal composition B, dioxone and human chorionic gonadotropin in a ratio of 6mL:2mg:500IU to obtain oxytocin 2;

[0058] (5) Selectively mature male fish were injected with oxytocin 1, and sexually mature female fish were injected with oxytocin 2 to obtain oxytocin-induced broodstock. The oxytocin-induced broodstock were cultured in a pond with a water temperature of 29℃, a salinity of 20‰, and a dissolved oxygen content of not less than 6mg / L. Each kilogram of male cichlids was injected with oxytocin 1, which consisted of 4mL of extract of Chinese medicine composition A and 1mg of dioxin. Each kilogram of female cichlids was injected with oxytocin 2, which consisted of 6mL of extract of Chinese medicine composition B, 2mg of dioxin, and 500IU of human chorionic gonadotropin.

[0059] (6) When the male fish’s genital opening protrudes into a cone shape and there is a noticeable bulge between the anus and the genital opening, and the female fish’s genital opening is round or oval, slightly red and slightly protruding, the abdomen is full and soft and the skin around the vent is loose, the sperm and fish egg cells are collected. Using the pads of the right thumb and index finger, start from the posterior edge of the pectoral fin and gently slide along both sides of the abdominal midline to 1 cm in front of the vent. Apply continuous, even and gradually increasing pressure, with the pressure limited to the smooth flow of eggs or sperm.

[0060] (7) Artificial insemination was performed on the collected semen and fish egg cells using a dry method at a volume ratio of 1:80;

[0061] (8) The obtained fertilized eggs were placed in an incubation device and incubated for 3 days in an environment with a water temperature of 23℃ and dissolved oxygen ≥7.0mg / L, and then cultured to obtain fish fry.

[0062] Example 3

[0063] A method for artificially breeding flower-bodied cichlids, the steps of which are as follows:

[0064] (1) Select healthy, disease-free, and well-developed broodstock of the flower-bodied cichlid, with males being over 24 months old and weighing ≥800g, and females being over 18 months old and weighing ≥500g. The ratio of males to females should be 1:3.

[0065] (2) Separate male and female broodstock into separate ponds for rearing. The water temperature in the rearing ponds is controlled at 25℃, the salinity is maintained at 20‰, and the dissolved oxygen content is not less than 6mg / L. During the rearing process of male and female broodstock in separate ponds, functional feed is added until 7 days before spawning. The daily feeding amount of functional feed is 0.08% of the body weight of the broodstock. The functional feed consists of 15 parts Epimedium, 10 parts Lycium barbarum, 8 parts Astragalus membranaceus, 0.3 parts Vitamin C, and 0.1 parts Vitamin E.

[0066] (3) Take 3 parts of Epimedium, 1 part of Cuscuta and 8 parts of Lycium barbarum, add 5 times the volume of water to the herbal composition A, then decoct twice, each time for 40 minutes, combine the two filtrates, and concentrate at 60℃ to 1 / 10 of the original volume to obtain the extract of herbal composition A; mix the extract of herbal composition A with dioxin at a ratio of 4 mL: 2 mg to obtain oxytocin 1;

[0067] (4) Take 1 part Angelica sinensis, 3 parts Ligustrum lucidum, 3 parts Rehmannia glutinosa and 5 parts Leonurus japonicus, add 7 times the volume of water, and then decoct twice, each time for 50 minutes. Combine the two filtrates and concentrate them at 60°C to 1 / 10 of the original volume to obtain the extract of the Chinese herbal composition B. Mix the extract of the Chinese herbal composition B, dioxone and human chorionic gonadotropin in a ratio of 3.5 mL: 3.8 mg: 550 IU to obtain oxytocin 2.

[0068] (5) Selectively mature male fish were injected with oxytocin 1, and sexually mature female fish were injected with oxytocin 2 to obtain oxytocin-induced broodstock. The oxytocin-induced broodstock were cultured in a pond with a water temperature of 29℃, a salinity of 20‰, and a dissolved oxygen content of not less than 6mg / L. Each kilogram of male cichlids was injected with oxytocin 1, which consisted of 4mL of extract of traditional Chinese medicine composition A and 2mg of dioxin. Each kilogram of female cichlids was injected with oxytocin 2, which consisted of 3.5mL of extract of traditional Chinese medicine composition B, 3.8mg of dioxin, and 550IU of human chorionic gonadotropin.

[0069] (6) When the male fish’s genital opening protrudes into a cone shape and there is a noticeable bulge between the anus and the genital opening, and the female fish’s genital opening is round or oval, slightly red and slightly protruding, the abdomen is full and soft and the skin around the vent is loose, the sperm and fish egg cells are collected. Using the pads of the right thumb and index finger, start from the posterior edge of the pectoral fin and gently slide along both sides of the abdominal midline to 1 cm in front of the vent. Apply continuous, even and gradually increasing pressure, with the pressure limited to the smooth flow of eggs or sperm.

[0070] (7) Artificial insemination was performed on the collected semen and fish egg cells using a dry method at a volume ratio of 1:100;

[0071] (8) The obtained fertilized eggs were placed in an incubation device and incubated for 4 days in an environment with a water temperature of 23℃ and dissolved oxygen ≥7.0mg / L, and then cultured to obtain fish fry.

[0072] Comparative Example 1

[0073] Similar to Example 1, only the operation of "adding functional feed during the separate rearing of male and female broodstock until 5 days before spawning, with the daily feeding amount of functional feed being 0.12% of the broodstock's body weight; the functional feed being 13.5 parts Epimedium, 12 parts Lycium barbarum, 6.5 parts Astragalus membranaceus, 0.2 parts Vitamin C, and 0.2 parts Vitamin E" was removed.

[0074] Comparative Example 2

[0075] Similar to Example 1, only the following steps were taken: (3) Take 2.2 parts of Epimedium, 2.5 parts of Cuscuta and 10 parts of Lycium barbarum, add 6 times the volume of water to the herbal composition A, then decoct twice, each time for 45 minutes, combine the two filtrates, and concentrate at 60°C to 1 / 10 of the original volume to obtain the extract of herbal composition A; mix the extract of herbal composition A with dioxin at a ratio of 3 mL: 1.8 mg to obtain oxytocin 1; (4) Take 1.5 parts of Angelica sinensis, 2.5 parts of Ligustrum lucidum, 4 parts of Rehmannia glutinosa and 8 parts of Leonurus japonicus, add 8 times the volume of water to the herbal composition B, then decoct twice, each time for 50 minutes, combine the two filtrates, and concentrate at 60°C to 1 / 10 of the original volume to obtain the extract of herbal composition B; mix the extract of herbal composition B, dioxin and human chorionic gonadotropin at a ratio of 5 mL: 3 mg: 650 IU. The proportions were mixed to obtain oxytocin 2; (5) Selectively mature male fish were injected with oxytocin 1, and sexually mature female fish were injected with oxytocin 2 to obtain oxytocin parent fish. The oxytocin parent fish were cultured in a pond with a water temperature of 29℃, a salinity of 20‰, and a dissolved oxygen content of not less than 6mg / L; each kilogram of male cichlid was injected with oxytocin 1 consisting of 3mL of extract of Chinese medicine composition A and 1.8mg of dioxin, and each kilogram of female cichlid was injected with oxytocin 2 consisting of 5mL of extract of Chinese medicine composition B, 3mg of dioxin and 650IU of human chorionic gonadotropin;” was replaced with “Selectively mature male fish were injected with dioxin, and sexually mature female fish were injected with dioxin and human chorionic gonadotropin; each kilogram of male cichlid was injected with 1.8mg of dioxin, and each kilogram of female cichlid was injected with 3mg of dioxin and 650IU of human chorionic gonadotropin.”

[0076] Comparative Example 3

[0077] Similar to Example 1, except that "the functional excipients are 13.5 parts Epimedium, 12 parts Lycium barbarum, 6.5 parts Astragalus membranaceus, 0.2 parts Vitamin C and 0.2 parts Vitamin E" is replaced with "the functional excipients are 13.5 parts Epimedium, 12 parts Lycium barbarum and 6.5 parts Astragalus membranaceus".

[0078] Comparative Example 4

[0079] Similar to Example 1, except that "take 1.5 parts of Angelica sinensis, 2.5 parts of Ligustrum lucidum, 4 parts of Rehmannia glutinosa and 8 parts of Leonurus japonicus, add 8 times the volume of water, then decoct twice, each time for 50 minutes, combine the two filtrates, and concentrate at 60°C to 1 / 10 of the original volume to obtain the extract of the herbal composition B" is replaced with "take 1.5 parts of Angelica sinensis and 4 parts of Rehmannia glutinosa, add 8 times the volume of water, then decoct twice, each time for 50 minutes, combine the two filtrates, and concentrate at 60°C to 1 / 10 of the original volume to obtain the extract of the herbal composition B".

[0080] Experimental Example 1

[0081] Two hundred healthy, disease-free, and well-developed male *Corydalis fasciatus* fish, aged 24 months and weighing 800-820g, and five hundred healthy, disease-free, and well-developed female *Corydalis fasciatus* fish, aged 20 months and weighing 500-520g, were selected. They were randomly divided into 5 groups of 140 fish each, and cultured according to the artificial breeding methods for *Corydalis fasciatus* described in Example 1 and Comparative Examples 1-4, respectively. The number of fertilized eggs, hatched fry, and fingerlings was counted, as shown in Table 1.

[0082] Fertilization rate (%) = Number of fertilized eggs / Number of eggs laid × 100%

[0083] Hatching rate (%) = Number of hatched fry / Number of fertilized eggs × 100%

[0084] Survival rate (%) = Number of fry / Number of hatched larvae × 100%

[0085] Table 1 shows the breeding effects of the breeding methods described in Example 1 and Comparative Examples 1-4.

[0086] Group Fertilization rate / % Hatching rate / % Seedling survival rate / % Example 1 88 87 70 Comparative Example 1 68 80 59 Comparative Example 2 43 72 59 Comparative Example 3 74 81 63 Comparative Example 4 61 77 56

[0087] As shown in Table 1, the breeding method for *Cyprinus fasciatus* described in this invention yields the best breeding results, with fertilization and hatching rates both exceeding 80%, and a fry survival rate as high as 70%. In contrast, the breeding methods described in Comparative Examples 1-4 all have a certain impact on the fertilization, hatching, and fry survival rates of *Cyprinus fasciatus*. This indicates that the functional excipients and spawning accelerators of this invention promote the generation and quality improvement of sperm or egg cells, and their spawning-inducing effect is relatively stronger.

[0088] In summary, the technical solution described in this invention can enhance the reproductive performance of *Cichlida fasciata*, promote the production of parent fish eggs or sperm, improve the quality of eggs or sperm, and thus improve the quality of fertilized eggs and the hatching rate. While enhancing the spawning-inducing effect, it also reduces the use of chemical substances, thereby reducing drug residues and environmental pollution. The breeding method described in this invention lays a solid foundation for subsequent breeding work, which is of great significance for the artificial breeding and large-scale farming of *Cichlida fasciata*.

[0089] The above description is only a preferred embodiment of the present invention. It should be noted that for those skilled in the art, several improvements and modifications can be made without departing from the principle of the present invention, and these improvements and modifications should also be considered within the scope of protection of the present invention.

Claims

1. A method for artificially breeding flower-bodied cichlids, characterized in that, Includes the following steps: (1) Select healthy, disease-free, and well-developed gonadal broodstock of 18 months or older, and raise male and female broodstock in separate ponds with a male-to-female ratio of 1:(2-3). (2) Selectively mature male fish were injected with spawning induced agent 1, and sexually mature female fish were injected with spawning induced agent 2 to obtain spawning broodstock. (3) Collect the semen of the induced male fish and the egg cells of the induced female fish and perform artificial insemination. After hatching, the fish fry are obtained.

2. The method for artificially breeding *Cyprinus floridae* according to claim 1, characterized in that, During the separate rearing of male and female parent fish in step (1), functional supplements are added until 5-7 days before spawning induction. The daily feeding amount of the functional feed is 0.08-0.2% of the body weight of the broodstock.

3. The method for artificially breeding *Cyprinus floridae* according to claim 2, characterized in that, The functional excipients include 10-15 parts Epimedium, 10-15 parts Lycium barbarum, 5-8 parts Astragalus membranaceus, 0.1-0.3 parts Vitamin C, and 0.1-0.3 parts Vitamin E.

4. The method for artificially breeding *Cyprinus floridae* according to claim 1, characterized in that, The oxytocin 1 comprises a traditional Chinese medicine composition A and dioxone in a dosage ratio of (2-4) mL:(1-3) mg; The traditional Chinese medicine composition A comprises the following components in parts by weight: 1-3 parts Epimedium, 1-3 parts Cuscuta, and 8-12 parts Lycium barbarum.

5. The method for artificially breeding *Cyprinus floridae* according to claim 1, characterized in that, The oxytocin 2 consists of a traditional Chinese medicine composition B comprising a dosage ratio of (3-6) mL:(2-4) mg:(500-800) IU, dioxone, and human chorionic gonadotropin; The traditional Chinese medicine composition B comprises the following components in parts by weight: 1-2 parts Angelica sinensis, 2-3 parts Ligustrum lucidum, 3-5 parts Rehmannia glutinosa, and 5-10 parts Leonurus japonicus.

6. The method for artificially breeding *Cyprinus floridae* according to claim 1, characterized in that, The broodstock described in step (2) are cultured in a pool with a water temperature of 26-29℃, a salinity of 19-23‰, and a dissolved oxygen content of not less than 6mg / L.

7. The method for artificially breeding *Cyprinus floridae* according to claim 1, characterized in that, In step (3), the volume ratio of the male fish semen to the female fish egg cells is 1:(50-100).