Anti-inflammatory composition as well as product and application thereof

Abstract

The invention provides an anti-inflammatory composition as well as a product and application thereof, and relates to the technical field of cosmetics. According to the application, research finds that the composition of the pyrus ussuriensis fruit fermentation liquor and the hyaluronic acid or the salt thereof has an excellent anti-inflammatory effect, and the synergistic anti-inflammatory effect between the pyrus ussuriensis fruit fermentation liquor and the hyaluronic acid or the salt thereof is found for the first time. In addition, the composition is high in safety and has no potential adverse reaction to a human body. The invention not only provides a new solution for resisting skin inflammation, but also has important significance for promoting regional economic development.

Description

Technical Field

[0001] This application relates to the field of cosmetic technology, and more particularly to an anti-inflammatory composition, its products, and applications. Background Technology

[0002] As a vital immune organ, the skin protects the body from external stimuli. When immune cells in the skin fight off these stimuli, inflammatory factors are released, triggering an inflammatory response. This inflammatory response is a natural defense mechanism the body uses to restore and maintain homeostasis after injury. However, excessively strong or prolonged inflammatory responses are harmful, damaging the skin barrier. Once the skin barrier is damaged, it cannot resist external stimuli, further exacerbating the inflammatory response. This vicious cycle leads to various skin problems, including acute inflammatory reactions such as redness, pain, or itching (e.g., peeling, dryness, and redness caused by acid or retinol peels); and chronic inflammatory reactions such as recurring redness, itching, flaking, or pigmentation, leading to skin sensitivity, darkening, accelerated aging, and more, including red pimples and acne scars. Therefore, providing products that alleviate inflammatory responses is crucial for relieving skin discomfort, improving skin appearance, and aiding in the restoration of the skin barrier, thus restoring or maintaining skin homeostasis.

[0003] While high molecular weight hyaluronic acid or its salts possess excellent moisturizing and anti-inflammatory effects, their high viscosity means that higher concentrations can create a sticky feeling when applied, hindering rapid skin absorption and thus limiting their efficacy. To compensate for this, the use of lower molecular weight hyaluronic acid or its salts in combination has been proposed. Due to their refreshing feel and rapid transdermal absorption, these combinations are believed to enhance moisturizing effects. However, this study found that the aforementioned strategy for compensating for moisturizing effects is not applicable to anti-inflammatory effects; that is, combining higher molecular weight hyaluronic acid or its salts with lower molecular weight hyaluronic acid or its salts does not synergistically enhance anti-inflammatory efficacy.

[0004] The technical problem this invention aims to solve is how to achieve good moisturizing effects while also enhancing anti-inflammatory efficacy using low-content, high-molecular-weight hyaluronic acid or its salts. Summary of the Invention

[0005] Therefore, after in-depth research, the inventors of this application discovered that combining the fermentation product filtrate of Pyrus ussuriensis fruit with hyaluronic acid or its salt with a molecular weight of 10k-3000kDa can achieve a synergistic effect in anti-inflammatory efficacy, thus completing this invention.

[0006] On one hand, this application provides an anti-inflammatory composition comprising, in a physiologically acceptable medium:

[0007] (a) At least one hyaluronic acid or its salt with a molecular weight of 10k-3000kDa.

[0008] (b) Fermented liquid of Pyrus ussuriensis fruit.

[0009] The mass ratio of (a) to (b) is (0.0003-0.15):1.

[0010] The mass ratio of (a) to (b) can be 0.0003:1, 0.0004:1, 0.0005:1, 0.0006:1, 0.0007:1, 0.0008:1, 0.0009:1, 0.001:1, 0.002:1, 0.003:1, 0.004:1, 0.005:1, 0.006:1, 0.007:1, or 0. Any value from 008:1, 0.009:1, 0.01:1, 0.0125:1, 0.02:1, 0.025:1, 0.03:1, 0.04:1, 0.05:1, 0.06:1, 0.07:1, 0.08:1, 0.09:1, 0.1:1, 0.11:1, 0.12:1, 0.13:1, 0.14:1, 0.15:1.

[0011] Preferably, the mass ratio of (a) to (b) is (0.0003-0.1):1.

[0012] The molecular weight of (a) can be any value among 10kDa, 20kDa, 30kDa, 40kDa, 41kDa, 50kDa, 60kDa, 70kDa, 80kDa, 90kDa, 100kDa, 200kDa, 300kDa, 400kDa, 500kDa, 600kDa, 700kDa, 800kDa, 900kDa, 998kDa, 1000kDa, 1100kDa, 1200kDa, 1300kDa, 1400kDa, 1500kDa, 1600kDa, 1700kDa, 1800kDa, 1870kDa, 1900kDa, 2000kDa, and 3000kDa.

[0013] Further, the hyaluronic acid or its salt includes one or more of hyaluronic acid, sodium hyaluronate, potassium hyaluronate, and calcium hyaluronic acid; preferably, the hyaluronic acid or its salt is sodium hyaluronate.

[0014] Furthermore, the molecular weight of (a) is 10k-2000kDa.

[0015] Furthermore, the fermentation broth of the autumn pear (Pyrus ussuriensis) is a fermentation filtrate of pear fruit.

[0016] Specifically, the fermentation broth of Pyrus ussuriensis fruit is a filtrate of grape juice fermented with Hansenula polymorpha yeast / Pyrus ussuriensis fruit.

[0017] Furthermore, the fermentation broth of the autumn pear (Pyrus ussuriensis) is obtained by fermenting grape juice with Hansenula polymorpha yeast.

[0018] Furthermore, the pear fermentation filtrate is obtained by fermenting pears with grape juice containing Hansenula polysaccharide.

[0019] Preferably, the grape juice Hansenula polymorpha is grape juice Hansenula polymorpha ZS02.

[0020] Preferably, the fermentation includes fermentation at a temperature of 25℃-40℃ and a fermentation speed of 100-300 rpm for 40-60 hours.

[0021] Specifically, the fermentation temperature can be any of the following values: 25℃, 26℃, 27℃, 28℃, 29℃, 30℃, 31℃, 32℃, 33℃, 34℃, 35℃, 36℃, 37℃, 38℃, 39℃, and 40℃. The fermentation speed can be 100 rpm, 110 rpm, 120 rpm, 130 rpm, 140 rpm, 150 rpm, 160 rpm, 170 rpm, 180 rpm, 190 rpm, 200 rpm, and 210 rpm. The fermentation time can be any of the following speed values: 220rpm, 230rpm, 240rpm, 250rpm, 260rpm, 270rpm, 280rpm, 290rpm, 300rpm, and 40h, 41h, 42h, 43h, 44h, 45h, 46h, 47h, 48h, 49h, 50h, 51h, 52h, 53h, 54h, 55h, 56h, 57h, 58h, 59h, 60h, and 40h.

[0022] In a preferred embodiment, the pear fermentation filtrate can be prepared by referring to the method described in Chinese Patent CN117165459A.

[0023] In a preferred embodiment, the method for preparing the pear fermentation filtrate includes:

[0024] Step 1: Crush and filter the pears to obtain pear juice;

[0025] Step 2: Sterilize the original juice of pears to obtain the fermentation medium;

[0026] Step 3: Inoculate the grape juice with Hanseniaspora uvarum ZS02 into the fermentation medium and ferment for 40-60 hours at 25℃-40℃ and 100-300rpm to obtain pear fermentation filtrate.

[0027] Preferably, the preparation method may further include the steps of filtering and removing impurities from the pear fermentation filtrate.

[0028] The grape juice contained Hanseniaspora uvarum ZS02, which was deposited on November 7, 2022, at the China General Microbiological Culture Collection Center (CGMCC) with accession number CGMCCNO.26056 and address at No. 3, Courtyard 1, Beichen West Road, Chaoyang District, Beijing.

[0029] In a preferred embodiment, the method for preparing the pear fermentation product filtrate includes:

[0030] 1) Crush the ripe pears, filter them with gauze to obtain the juice, and then filter them sequentially through a 2.0μm clarifying plate, a 0.8μm clarifying plate, and a 0.22μm filter membrane to obtain the original pear juice;

[0031] 2) Sterilize the original juice of pear fruit at 100℃ for 10 minutes to obtain pear fruit juice filtrate;

[0032] 3) Inoculate a single colony of Hanseniaspora uvarum ZS02 into the grape juice filtrate and culture it in a shake flask at 200 rpm and 30℃ for 48 h to obtain the grape juice fermentation filtrate.

[0033] 4) Centrifuge the pear fermentation filtrate at 6000 rpm for 10 min, filter to remove bacteria, take the supernatant and sterilize at 80℃ for 1 h, then filter with a 0.22 μm filter membrane to finally obtain the pear fermentation product filtrate.

[0034] Those skilled in the art may also choose to purchase the grape juice fermentation product filtrate of *Hansenula polysaccharide* / *Pyrus ussuriensis* prepared by Bloomage Biotechnology Co., Ltd. for experiments. The source of this product is not specifically limited in this application.

[0035] Pear, also known as peeled pear, is a local cultivar of the Pyrus suriensis Maxim., belonging to the genus Pyrus in the Rosaceae family. Pears are rich in nutrients such as free acids, pectin, polysaccharides, fats, proteins, cellulose, iron, calcium, and phosphorus, earning them the reputation of being the "king of ginkgo nuts." Due to their warm nature and sweet, slightly sour taste, they possess properties that clear heat and resolve phlegm, nourish the stomach and lungs, quench thirst and relieve coughs, soften blood vessels, and alleviate hangovers. They are a green food rich in nutrients.

[0036] This application is the first to discover that a combination of grape juice containing Hansenula polymorpha / Pyrus pyrifolia fermentation product filtrate and hyaluronic acid or its salt with a molecular weight of 10k-3000kDa has a synergistic anti-inflammatory effect, which can still achieve good anti-inflammatory efficacy with low component dosage. This also provides an effective solution for achieving excellent moisturizing and anti-inflammatory effects at the same time.

[0037] Furthermore, the physiologically acceptable medium includes one or more of solvents, pH adjusters, preservatives, and thickeners.

[0038] This application may also include other functional ingredients that do not have a negative impact on anti-oxidation, such as hyaluronic acid or its salts.

[0039] Furthermore, the molecular weight of the hyaluronic acid or its salt is less than 10 kDa. Specifically, it can be any value among 379 kDa, 400 kDa, 500 kDa, 600 kDa, 700 kDa, 800 kDa, 900 kDa, 1 kDa, 2 kDa, 3 kDa, 4 kDa, 5 kDa, 6 kDa, 7 kDa, 8 kDa, 8.812 kDa, 9 kDa, 9.9 kDa, 9.99 kDa, and 9.999 kDa.

[0040] This application may also add physiologically acceptable excipients, which may be suitable propellants, solubilizers, cosolvents, emulsifiers, colorants, binders, disintegrants, fillers, lubricants, wetting agents, osmotic pressure regulators, stabilizers, flow aids, flavoring agents, suspending agents, coating materials, fragrances, anti-adhesion agents, binding agents, penetration enhancers, buffers, plasticizers, surfactants, foaming agents, defoamers, encapsulating agents, humectants, absorbents, diluents, flocculants and anti-flocculation agents, filter aids, release inhibitors, etc.

[0041] The compositions of this application can be prepared by general methods, wherein one or more physiologically acceptable media or carriers may be added. Those skilled in the art can select the media or carrier according to actual needs.

[0042] In a preferred embodiment, the medium may be water.

[0043] In a preferred embodiment, the method for preparing the composition includes: mixing (a) and (b) in a physiologically acceptable medium, wherein the mass ratio of (a) to (b) is (0.0003-0.15):1, and the molecular weight of (a) is 10k-3000kDa.

[0044] One aspect of this application relates to compositions, which are any compositions capable of achieving the effects described in this application. The compositions include, but are not limited to, the simultaneous or sequential use of the components. "Simultaneous use" includes using them together in the same formulation or separately in different formulations. "Sequential use" includes using them sequentially in different formulations, with no restriction on the order of sequential use.

[0045] On the other hand, this application also provides the use of the described composition in the preparation of anti-inflammatory products.

[0046] Furthermore, the anti-inflammatory product is used to prevent and / or alleviate skin problems caused by inflammation.

[0047] Furthermore, the anti-inflammatory products include skin care products that prevent and / or alleviate skin problems caused by inflammation.

[0048] Preferably, the skin problem includes one or more of the following: impaired skin barrier, skin redness and swelling, skin pain, skin itching, skin peeling, and skin pigmentation.

[0049] Preferably, the anti-inflammatory product includes a product that regulates the expression level of inflammatory factors, which include one or more of IL-6, IL-1β, and TNF-α.

[0050] More preferably, the regulation includes inhibiting the expression of inflammatory factors and reducing the expression level of inflammatory factors.

[0051] On the other hand, this application also provides an anti-inflammatory product comprising the aforementioned composition.

[0052] Furthermore, the product is a topical skin preparation.

[0053] On the other hand, this application also provides a skin care product comprising the aforementioned composition.

[0054] Furthermore, the skin care product is a topical skin preparation.

[0055] Preferably, the product or composition is mild and non-irritating to the human body and meets the requirements of cosmetic safety technical specifications.

[0056] Those skilled in the art can adjust the concentration of the composition or product used in this application according to the actual situation, and no specific limitation is made in this application.

[0057] In a preferred embodiment, the concentration of the fermented Pyrus ussuriensis fruit broth in the product, by mass percentage, can be 0.01%-50%, for example, any value or range of 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, 6.5%, 7%, 7.5%, 8%, 8.5%, 9%, 9.5%, 10%, 20%, 30%, 40%, 50%.

[0058] In a preferred embodiment, the concentration of hyaluronic acid or its salt in the product, by mass percentage, can be 0.001%-5%, for example, any value or range of 0.001%, 0.002%, 0.003%, 0.004%, 0.005%, 0.01%, 0.02%, 0.03%, 0.04%, 0.05%, 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%.

[0059] The present invention has the following beneficial effects:

[0060] The grape juice provided in this application contains a synergistic anti-inflammatory effect with a combination of Hansenula polymorpha / Pyrus pyrifolia fermentation product filtrate and hyaluronic acid or its salt with a molecular weight of 10k-3000kDa, thereby achieving good anti-inflammatory efficacy even with reduced component dosage.

[0061] Furthermore, excellent anti-inflammatory effects can still be achieved when using low concentrations of high molecular weight hyaluronic acid or its salts, providing an effective solution for achieving both superior efficacy and a better skin feel.

[0062] Furthermore, this composition is highly safe and has no potential adverse reactions in humans. It also holds significant importance for promoting regional economic development. Detailed Implementation

[0063] To more clearly illustrate the overall concept of this application, a detailed description is provided below by way of embodiments. Numerous specific details are set forth in the following description to provide a more thorough understanding of the invention. However, it will be apparent to those skilled in the art that the invention can be practiced without one or more of these details. In other instances, certain technical features well-known in the art have not been described to avoid confusion with the invention.

[0064] It should be noted that the following detailed descriptions are illustrative and intended to provide further explanation of this application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains.

[0065] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to this application. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0066] Unless otherwise specified, in the following embodiments, reagents or instruments whose manufacturers are not indicated are all conventional products that can be purchased commercially.

[0067] The experimental materials involved included: grape juice Hansenula polymorpha / Pear fruit fermentation product filtrate (Pear fruit fermentation filtrate, batch number: 20220706, Bloomage Biotechnology Co., Ltd.), sodium hyaluronate (1.87 million Daltons, batch number: 1807196; 998,000 Daltons, batch number: 201230283; 41,000 Daltons, batch number: 22050642; 8,812 Daltons, batch number: 22032442, Bloomage Biotechnology Co., Ltd.), mouse macrophages Raw264.7, RPMI 1640 medium (KGI Biotechnology), fetal bovine serum (Gibco), CCK-8 cytotoxicity and cell proliferation kit (proteintech), Mouse TNF-α ELISA Kit (proteintech), Mouse IL-6 ELISA Kit (proteintech), Mouse IL-1β ELISA Kit (proteintech), and Lactobacillus / Pear juice fermentation product filtrate were purchased from Guangzhou Tongyi Chemical Co., Ltd.

[0068] The instruments and equipment mentioned below include: a clean bench (Beijing Donglian Haer Instrument Manufacturing Co., Ltd., SCB-1520), a carbon dioxide incubator (SANYO), an inverted microscope (OLYMPUS, CKX41), a digital display constant temperature water bath (Jintan Zhongda Instrument Factory), and a constant temperature microplate rapid shaker (Haimen Qilin Medical Instrument Factory).

[0069] Before further describing specific embodiments of the present invention, it should be understood that the scope of protection of the present invention is not limited to the specific embodiments described below; it should also be understood that the terminology used in the embodiments of the present invention is for describing specific embodiments and not for limiting the scope of protection of the present invention. Test methods in the following embodiments that do not specify specific conditions are generally performed under conventional conditions or as recommended by the respective manufacturers.

[0070] When numerical ranges are given in the embodiments, it should be understood that, unless otherwise stated in the present invention, both endpoints of each numerical range and any value between the two endpoints may be selected. Unless otherwise defined, all technical and scientific terms used in this invention have the same meaning as commonly understood by one of ordinary skill in the art. In addition to the specific methods, apparatus, and materials used in the embodiments, based on the knowledge of the prior art possessed by one of ordinary skill in the art and the description of this invention, any prior art methods, apparatus, and materials similar to or equivalent to those described, apparatus, and materials in the embodiments of this invention may be used to implement the present invention.

[0071] Unless otherwise stated, the experimental methods, detection methods, and preparation methods disclosed in this invention all employ conventional techniques in the fields of microbiology, biochemistry, analytical chemistry, cell culture, and related areas.

[0072] In addition, the "water" mentioned in this invention includes any feasible water that can be used in the art, such as deionized water, distilled water, ion-exchanged water, double-distilled water, high-purity water, and purified water.

[0073] In the following examples, unless otherwise specified, % means wt%, that is, weight percentage, and parts means mass parts.

[0074] Experiment Example 1: Anti-inflammatory Effect Experiment

[0075] 1.1 Evaluation of anti-inflammatory efficacy

[0076] This experiment used a mouse macrophage cell line as the model cell. LPS stimulation was used to induce macrophages to secrete inflammatory factors. The samples were then treated, and the anti-inflammatory efficacy of the samples was investigated by quantitatively detecting the expression of inflammatory factors in the culture supernatant.

[0077] 1.2 Solution Preparation

[0078] LPS (lipopolysaccharide) reaction solution: Prepare a stock solution with a concentration of 100 μg / mL using serum-free RPMI 1640 medium, filter it through a 0.22 μm filter membrane for sterilization, store it at -20℃, and dilute it to 1 μg / mL before use.

[0079] Sample solution:

[0080] Fermentation filtrate: Add the fermentation product filtrate of grape juice containing Hansenula polysaccharide / Pyrus ussuriensis or the fermentation product filtrate of Lactobacillus / pear juice to serum-free culture medium, mix and stir until completely dissolved to obtain fermentation filtrates of different concentrations (0.5%, 1%, 2%), and filter with a 0.22μm filter membrane for sterilization.

[0081] Sodium hyaluronate: Sodium hyaluronate with different molecular weights (8812 Daltons, 41,000 Daltons, 998,000 Daltons, 1,870,000 Daltons) was added to serum-free culture medium, mixed and stirred until completely dissolved, to obtain sodium hyaluronate with different concentrations (0.0006%, 0.001%, 0.01%, 0.025%, 0.04%, 0.2%, 0.025%, 0.0004%), which was then filtered through a 0.22 μm filter membrane for sterilization.

[0082] Composition: Prepared according to the contents in Table 1, adding sodium hyaluronate and either the filtrate of *Hansenula polymorpha* / *Pyrus ussuriensis* fermentation product or the filtrate of *Lactobacillus* / *Pear* fermentation product to the mixture.

[0083] The sample solution was completely dissolved by mixing and stirring in the LPS reaction solution, and then filtered through a 0.22μm filter membrane for sterilization.

[0084] 1.3 Paving

[0085] Log-growing mouse macrophages (Raw264.7 cells) were harvested at a concentration of 5 × 10⁻⁶ cells / year. 4 Cells were seeded at a density of 1 mL / well in 24-well plates. The culture medium was RPMI 1640 medium supplemented with 10% fetal bovine serum. The seeded cells were incubated in a CO2 incubator at 37°C with 5% CO2 for 24 hours.

[0086] 1.4 Chemical Dosing Treatment

[0087] Raw264.7 cells were fed at a rate of 1×10⁻⁶. 5 / mL was inoculated into 24-well plates and cultured at 37℃ and 5% CO2 for 24h. 1mL of sample solution was added to the test group, while the group with LPS reaction solution was used as the model (control) group, and the culture was continued for another 24h.

[0088] 1.5 test

[0089] Cell supernatant was collected and centrifuged. The levels of inflammatory factors (IL-6, IL-1β, TNF-α) were detected according to the ELISA kit instructions, and the inhibition rate was calculated. The results are shown in Table 2-4.

[0090] Inhibition rate (%) = (average content of inflammatory factors in the model group - average content of inflammatory factors in the test substance) / average content of inflammatory factors in the model group × 100%.

[0091] 1.6 Results Analysis

[0092] Compared with the control group, the t-test was used. "ns" indicates no statistical difference (p>0.05); "*" indicates a statistical difference (p<0.05); "**" indicates a significant statistical difference (p<0.01); "***" indicates an extremely significant statistical difference (p<0.001).

[0093] To illustrate the additive effect of the composition, we used the King's formula: Q=E(a+b) / (Ea+Eb-Ea×Eb) (where Q<0.55 indicates significant antagonism, Q=0.55-0.85 indicates antagonism, Q=0.85-1.15 indicates additive effect, and Q>1.15 indicates enhancement).

[0094] Table 1

[0095]

[0096]

[0097] Table 2

[0098]

[0099] Note: The fermentation filtrate group uses the same fermentation filtrate as described in the corresponding examples or comparative examples as the sample solution; the sodium hyaluronate group uses the same sodium hyaluronate as described in the corresponding examples or comparative examples as the sample solution; and the composition group uses the compositions described in Examples 1-11 and Comparative Examples 1-3 as the sample solution.

[0100] Table 3

[0101]

[0102]

[0103] Note: The fermentation filtrate group uses the same fermentation filtrate as described in the corresponding examples or comparative examples as the sample solution; the sodium hyaluronate group uses the same sodium hyaluronate as described in the corresponding examples or comparative examples as the sample solution; and the composition group uses the compositions described in Examples 1-11 and Comparative Examples 1-3 as the sample solution.

[0104] Table 4

[0105]

[0106] Note: The fermentation filtrate group uses the same fermentation filtrate as described in the corresponding examples or comparative examples as the sample solution; the sodium hyaluronate group uses the same sodium hyaluronate as described in the corresponding examples or comparative examples as the sample solution; and the composition group uses the compositions described in Examples 1-11 and Comparative Examples 1-3 as the sample solution.

[0107] Conclusion: As shown in Tables 2-4, the combination of grape juice fermentation product filtrate of *Hansenula polysaccharide* / *Pyrus pyrifolia* and sodium hyaluronate of a specific molecular weight has anti-inflammatory effects. Compared with the use of grape juice fermentation product filtrate of *Hansenula polysaccharide* / *Pyrus pyrifolia* or sodium hyaluronate alone, the combination has a more significant inhibitory effect on inflammatory factors (IL-6, IL-1β, TNF-α), and plays a synergistic role in anti-inflammation.

[0108] Experimental Example 2: Patch Test

[0109] The patch test in this embodiment was conducted in accordance with the "Human Skin Patch Test" in the Cosmetic Safety Technical Specifications (2015 Edition). Specifically, the methods included are as follows:

[0110] Subject Recruitment: We are recruiting 30 healthy subjects, regardless of gender.

[0111] Instrumentation: Patch tester (Chemotechnique Diagnostics AB, Sweden).

[0112] Sample preparation: According to the contents in Table 5, add sodium hyaluronate and grape juice Hansenula polysaccharide / Pyrus ussuriensis fruit fermentation product filtrate to purified water, mix and stir until completely dissolved to obtain the sample solution.

[0113] Preparation of the control group: Purified water was used as the control group.

[0114] Experimental Method: Tear open the packaging of the plaque applicator and measure 0.020 g of each of samples 1-11 into the small chamber. Apply the plaque applicator to the flexor side of the subject's forearm, and gently press it with the palm of your hand to ensure even application to the skin, for 24 hours. After removing the plaque applicator, observe and record the reaction results according to Table 6 for 48 hours. The results are shown in Table 7.

[0115] Table 5

[0116]

[0117] Table 6. Grading Criteria for Skin Reactions in Closed Patch Tests

[0118]

[0119] Table 7

[0120] Group Level 0 Level 1 Level 2 Level 3 Level 4 Sample 1 30 0 0 0 0 Sample 2 30 0 0 0 0 Sample 3 30 0 0 0 0 Sample 4 30 0 0 0 0 Sample 5 30 0 0 0 0 Sample 6 30 0 0 0 0 Sample 7 30 0 0 0 0 Sample 8 30 0 0 0 0 Sample 9 30 0 0 0 0 Sample 10 30 0 0 0 0 Sample 11 30 0 0 0 0 control group 30 0 0 0 0

[0121] Conclusion: As shown in Table 7, samples 1-11 all showed negative reactions and were mild and non-irritating to the human body.

[0122] The above description is merely an embodiment of this application and is not intended to limit the scope of this application. Various modifications and variations can be made to this application by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of this application should be included within the scope of the claims of this application.

Claims

1. An anti-inflammatory composition, characterized in that, The composition comprises, in a physiologically acceptable medium, the following: (a) At least one hyaluronic acid or its salt with a molecular weight of 10k-3000kDa. (b) Fermented liquid of Pyrus ussuriensis fruit. The mass ratio of (a) to (b) is (0.0003-0.15):

1.

2. The composition according to claim 1, characterized in that, The molecular weight of (a) is 10k-2000kDa.

3. The composition according to claim 1, characterized in that, (b) is the fermented filtrate of pear fruit.

4. The composition according to claim 3, characterized in that, (b) is obtained by fermenting grape juice with Hansenula polysaccharide yeast to obtain pear fruit.

5. Use of the composition according to any one of claims 1-4 in the preparation of anti-inflammatory products.

6. The application according to claim 5, characterized in that, The anti-inflammatory products include skin care products that prevent and / or alleviate skin problems caused by inflammation.

7. The application according to claim 6, characterized in that, The skin problems mentioned include one or more of the following: impaired skin barrier, redness and swelling, pain, itching, peeling, and pigmentation.

8. A skin care product, characterized in that, The skin care product comprises the composition according to any one of claims 1-4.

9. The skin care product according to claim 8, characterized in that, The skin care product is a topical preparation for skin use.

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