Creatine magnesium chelate as well as preparation method and application thereof

Abstract

The invention belongs to the technical field of nutritional supplement chemical and chemical synthesis, and particularly relates to a creatine magnesium chelate and a preparation method and application thereof, and the preparation method comprises the steps of reaction solution preparation, mixing, chelation reaction, thermal microfiltration, anti-solvent crystallization, centrifugal recovery and vacuum drying. The method comprises the following steps: carrying out a low-temperature equimolar chelation reaction on creatine monohydrate and a specific magnesium salt under the conditions that the pH value is 7.0-8.0 and the temperature is 25-40 DEG C, carrying out thermal microfiltration on a solution after the reaction is completed through a microfiltration column, then cooling a filtered solution to 5-10 DEG C, carrying out an ethanol and acetone binary anti-solvent low-temperature precipitation purification process, centrifugally collecting crystals, and carrying out vacuum drying, so that the product purity is more than or equal to 98%, the yield is more than or equal to 85%, and the product purity is more than or equal to 90%. The obtained product shows a stable coordination structure, and is especially suitable for the production of sports nutritional supplements and medical magnesium supplements.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of nutritional supplement chemistry and chemical synthesis, and particularly relates to a magnesium creatine chelate, a preparation method and application thereof. BACKGROUND

[0002] Creatine is a well-known supplement that is found primarily in muscle in the human body and is involved in energy metabolism. Creatine helps generate ATP, which is the primary source of energy for cells, especially during high-intensity, short-duration exercise such as weight lifting or sprinting, and creatine can rapidly replenish ATP and delay fatigue. Therefore, creatine supplements are popular among athletes to enhance athletic performance and muscle mass. However, creatine is prone to cyclization, and under acidic conditions, creatine has a tendency to form creatinine, and obviously, exposure of creatine to the acidic environment of the stomach will lead to irreversible formation of creatinine, which will affect any further biological use of the ingested creatine.

[0003] Magnesium is an essential trace element for the human body, involved in more than 300 enzyme reactions, and magnesium also plays a role in energy metabolism, involved in the synthesis and utilization of ATP, and is an important mineral involved in a variety of biochemical processes, including energy production and muscle function. However, the use of magnesium ion salts is largely ineffective, because most of the ingested element is lost in the acidic environment of the stomach.

[0004] Combining creatine and magnesium into a compound, magnesium creatine chelate, these chelates are easily absorbed in the intestine via mucosal cells by active transport, and by using amino acids as carrier molecules, the mineral is absorbed together with the amino acid as a single unit, which can provide synergistic benefits by enhancing the bioavailability and efficacy of both.

[0005] In the prior art, such as AU774654B2 / US6114379A, creatine + Mg 2+ Reacts at pH 7.5-10, followed by spray drying. This method results in low purity (≤ 94%) and high creatinine content (≥ 0.6%) due to high temperature drying. As CN103626669 A / CN106478438A, involves reacting at 70-80°C, ethanol precipitation, and vacuum drying at 60-80°C, however, these processes result in low purity and low yield of the product. SUMMARY

[0006] Therefore, the present application provides a magnesium creatine chelate, a preparation method and application thereof, to solve the technical problems of low purity, high creatinine content, and low yield of the existing methods for producing magnesium creatine chelate.

[0007] The technical solution of the present application to solve the above technical problems is as follows:

[0008] A method for preparing a magnesium creatine chelate, comprising the steps of:

[0009] S10. Preparing a reaction solution: dissolving creatine and magnesium salt in water respectively according to the proportion to prepare a creatine solution and a magnesium salt solution;

[0010] S20. Mixing: slowly mixing the magnesium salt solution and the creatine solution uniformly under continuous stirring to obtain a mixture;

[0011] S30. Chelation reaction: continuously stirring the mixture until complete chelation;

[0012] S40. Hot microfiltration: hot microfiltration of the reaction solution completed in step S30 through a microfiltration column to remove particulate impurities;

[0013] S50. Anti-solvent crystallization: cooling the filtered solution in step S40 to 5-10℃, adding an anti-solvent to precipitate the magnesium creatine chelate, the anti-solvent being a binary anti-solvent of ethanol and acetone;

[0014] S60. Centrifugal recovery: collecting the crystals by centrifugation;

[0015] S70. Vacuum drying: vacuum drying to collect the product.

[0016] Preferably, in step S50, the volume ratio of the binary anti-solvent to the magnesium creatine chelate filtered solution is 0.4-0.6:1.

[0017] Preferably, in the anti-solvent of step S50, the volume ratio of the ethanol and acetone is 2.2-2.8:1.

[0018] Preferably, in step S10, the concentration of the creatine solution is 0.1 to 0.5 mol / L.

[0019] Preferably, in step S10, the magnesium salt is at least one of magnesium chloride, magnesium acetate, magnesium sulfate, magnesium citrate, magnesium salicylate, magnesium dihydrogen phosphate, magnesium nitrate, magnesium oxide, magnesium carbonate, magnesium hydroxide, magnesium phosphate, and magnesium oxalate.

[0020] Preferably, in step S10, the molar ratio of the creatine and magnesium salt solution is (1:0.97-1.05).

[0021] Preferably, in step S10, the magnesium salt is magnesium chloride hexahydrate.

[0022] Preferably, in step S30, the pH of the chelation reaction mixture is adjusted to between 7.0 and 8.0.

[0023] Preferably, in step S30, the temperature is maintained at 25-40℃ for 2-4 hours for the chelation reaction.

[0024] A creatine magnesium chelate prepared by the method of any one of the preceding claims.

[0025] Use of a creatine magnesium chelate as described above in sports nutrition supplements, medical magnesium supplements.

[0026] The technical scheme of the present application has the beneficial effects that:

[0027] The method for preparing the creatine magnesium chelate provided by the present application mainly comprises the following steps: preparing a reaction solution, mixing, chelation reaction, hot microfiltration, anti-solvent crystallization, centrifugal recovery, and vacuum drying. The water-phase low-temperature production process is adopted, i.e., low-temperature chelation reaction is carried out at 25-40 DEG C, and low-temperature ethanol and acetone binary anti-solvent precipitation is carried out at 5-10 DEG C. The process can realize a yield of greater than or equal to 85%, a purity of greater than or equal to 98%, and a creatinine content of less than or equal to 0.2%. The purity is improved by 4%-5%, and the creatinine content is reduced by about 10 times. The technical problems of low yield, high creatinine content, and low purity existing in the prior art methods for producing the creatine magnesium chelate are solved. BRIEF DESCRIPTION OF DRAWINGS

[0028] Figure 1 It is a process flow diagram of the creatine magnesium chelate.

[0029] Figure 2 It is a chemical structure diagram of the creatine magnesium chelate (O, N bidentate coordination). DETAILED DESCRIPTION

[0030] It should be noted that the embodiments in the present application and the features in the embodiments can be combined with each other without conflict, and the present application is not limited to the specific process steps and materials disclosed herein, because these process steps and materials can be changed to a certain extent. The terms used herein are only for the purpose of describing specific embodiments, and are not limiting, because the scope of the present application is only limited by the appended claims and their equivalents. The technical solutions of the present application will be further described below in combination with the drawings of the embodiments of the present application, and the present application is not limited to the following specific embodiments.

[0031] Reference Figure 1 and Figure 2 The present application is a metal chelate prepared by the method, which comprises a creatine ligand (creatine solution) bonded with a metal selected from Mg to form a chelate ring, and has a ligand-to-metal molar ratio of about 1:1. The chelate is formed by reacting creatine with a metal under reaction conditions conducive to the formation of the chelate. The creatine can be provided by a member of creatine hydrate, and the metal can be provided by magnesium in free elemental form or magnesium chloride, magnesium acetate, magnesium sulfate, magnesium citrate, magnesium salicylate, magnesium dihydrogen phosphate, magnesium nitrate, magnesium oxide, magnesium carbonate, magnesium hydroxide, magnesium phosphate, magnesium oxalate.

[0032] Generally, the method for preparing the creatine chelate of the present application is as follows:

[0033] First, creatine and magnesium salt are dissolved in water, respectively, for example, if magnesium is the metal to be chelated, magnesium chloride, magnesium acetate, magnesium sulfate, magnesium citrate, magnesium salicylate, magnesium dihydrogen phosphate, magnesium nitrate, magnesium oxide, magnesium carbonate, magnesium hydroxide, magnesium phosphate, magnesium oxalate, etc. can be used as the metal source, which will be dissolved in water, and then the creatine ligand (creatine solution) is added to the solution. If the pH level is not near neutral, a pH adjuster can be added, for example, preferably using sodium hydroxide solution to adjust the pH to between 7.0 and 8.0. It is important that the order of mixing the ingredients is not the focus of the present application, the creatine ligand can be added to water first, then the magnesium salt solution, or added at the same time.

[0034] In the above description, the molar ratio of ligand to metal is 1:1, if the molar ratio of ligand to metal is greater than 1:1, there can be another creatine anion.

[0035] For example, the creatine magnesium chelate can be prepared by first mixing equimolar creatine monohydrate and magnesium chloride hexahydrate in water, the water is deionized water, the concentration of the creatine solution is 0.1 to 0.5 mol / L, the molar ratio of creatine to magnesium salt is (1:0.97-1.05), and it is necessary to make the molar concentration of the creatine solution and the magnesium salt solution as close as possible.

[0036] Second, continue to stir the mixture (after mixing the magnesium salt solution and the creatine solution evenly) until complete chelation, adjust the pH of the mixture to between 7.0 and 8.0; specifically, use PID to control pH 7.5±0.05 (with 1 mol / L sodium hydroxide solution), adjust the pH of the mixture to between 7.0 and 8.0, preferably the pH is between 7.2 and 7.8, maintain the temperature at 25-40℃ for 2-4 hours, preferably incubate at 25-33℃ for 2-3 hours, monitor the free creatine with UV-198nm online to be ≤0.5%.

[0037] Third, the solution after the above reaction is complete is micro-filtered through a micro-filtration column to remove particulate impurities. Specifically, the reaction liquid is passed through a 35℃-40℃, 1 μm micro-filtration column, preferably 40℃, to remove particulate impurities.

[0038] Fourthly, the filtered solution is cooled to 5-10°C, and an anti-solvent is added to precipitate the creatine magnesium chelate, the anti-solvent being a binary anti-solvent of ethanol and acetone. Specifically, the filtrate is cooled to 5°C, and a binary anti-solvent is added to precipitate the creatine magnesium chelate, the volume ratio of the binary anti-solvent to the filtered solution of the creatine magnesium chelate being 0.4-0.6:1, the volume ratio of ethanol to acetone being 2.2-2.8:1, preferably, the volume ratio being 2.5:1, and if the induction time is >10 minutes, 0.1-1wt% of seed crystals of the creatine magnesium chelate filtered solution is added to induce crystallization.

[0039] Fifthly, the crystals are collected by centrifugation. Specifically, the centrifugal speed is 2000-2500rpm, and then the same proportion of cold anti-solvent (0.5V) is used for washing.

[0040] Sixthly, vacuum drying. Specifically, vacuum drying is performed at 40-50°C, ≤50mbar, until the weight is constant.

[0041] For example, in a preferred embodiment, the method for preparing the creatine chelate comprises the following steps:

[0042] Specifically, 14.915g (0.1mol) of creatine monohydrate is dissolved in 200ml of deionized water to prepare a 0.5mol / L creatine solution, and an equal mole of 20.33g (0.1mol) of magnesium chloride hexahydrate (MgCl2·6H2O) is dissolved in 200ml of deionized water to prepare a 0.5mol / L magnesium salt solution, under continuous stirring, the magnesium salt solution and the creatine solution are uniformly mixed, the pH of the reaction mixture is adjusted to 7.5±0.05 with 1mol / L sodium hydroxide solution, the mixture is continuously stirred, and the chelation reaction is carried out at a temperature of 30°C for 3 hours, the above-mentioned reaction completed solution is filtered through a 40°C, 1μm microfiltration column to remove particulate impurities, the filtrate is cooled to 5°C, and a binary anti-solvent of ethanol and acetone is added to precipitate the creatine magnesium chelate, the volume ratio of the binary anti-solvent to the filtered solution of the creatine magnesium chelate being 0.5:1, i.e. 200ml of binary anti-solvent is added to the above-mentioned 400ml filtered solution, wherein the volume ratio of ethanol to acetone is 2.5:1, i.e. 143ml of ethanol and 57ml of acetone are added, after the precipitation is completed, the crystals are collected by centrifugation, the centrifugal speed is 2500rpm, and then vacuum drying is performed at 40°C, 40mbar, and as a result, 20.3g of creatine magnesium chelate is obtained, with a yield of 90% (calculated based on the molecular weight of creatine magnesium chelate tetrahydrate 225.51g / mol).

[0043] It is worth noting that the process temperature and process time involved in the above-mentioned embodiments are a temperature or time adopted during the experiment, and those skilled in the art should make reasonable adjustments within the error range based on the process temperature and process time provided by the present application, which should be included in the protection scope of the present application.

[0044] The application also discloses a creatine magnesium chelate prepared by the preparation method.

[0045] The application also discloses application of the creatine magnesium chelate in sports nutrition supplements and medical magnesium supplements.

[0046] In order to make the purpose, technical scheme and advantages of the present application clearer, the present application is further described in detail through experimental examples.

[0047] It should be noted that all raw materials and reagents in the embodiments of the present application are commercially available and can be used without further purification.

[0048] The yield calculation formula of the creatine magnesium chelate sample is as follows:

[0049] Yield (%) = actual product mass / theoretical product mass x 100%.

[0050] 1. Raw materials and equipment (see Table 1)

[0051] Table 1 Details of raw materials and equipment

[0052]

[0053]

[0054] 2. Experimental materials:

[0055] The raw materials involved in the embodiments of the present application are standard preparations available on the market.

[0056] 3. Performance test method and instrument:

[0057] The performance test adopts GB / T26792-2019 “High Performance Liquid Chromatograph” and GB / T16631-2008 “General Rules for High Performance Liquid Chromatography”, and the purity and creatinine content of the creatine magnesium chelate are tested by using a high performance liquid chromatograph.

[0058] First group of experiments: exploring the influence of different types and amounts of anti-solvents on the yield, purity and creatinine content of the product

[0059] Take 14.915 grams (0.1 moles) of creatine monohydrate and dissolve it in 200 milliliters of deionized water to make a 0.5 mol / L creatine solution. Dissolve an equal amount of 20.33 grams (0.1 moles) of magnesium chloride hexahydrate in 200 milliliters of deionized water to make a 0.5 mol / L magnesium salt solution. Slowly mix the magnesium salt solution and the creatine solution uniformly under continuous stirring. Adjust the pH of the reaction mixture to 7.5 ± 0.05 with 1 mol / L sodium hydroxide solution. Continue to stir the mixture and maintain the temperature at 30°C for 3 hours of chelation reaction. Filter the above reaction solution through a 40°C, 1 μm microfiltration column to remove particulate impurities. Cool the filtrate to 5°C and add different types and amounts of anti-solvents (see Table 2) to precipitate the magnesium creatine chelate. After the precipitation is complete, collect the crystals by centrifugation at 2500 rpm. Then vacuum dry at 40°C and 40 mbar.

[0060] Take the product mass and calculate the yield (based on the molecular weight of magnesium creatine chelate tetrahydrate of 225.51 g / mol). Test the purity and creatinine content of the magnesium creatine chelate using a high-performance liquid chromatograph. The results are shown in the table below:

[0061] Table 2 Comparison of product yield, purity, and creatinine content with different types and amounts of anti-solvents

[0062]

[0063] As can be seen from the above table, when other raw materials are the same and the preparation method is consistent, changing the type and amount of anti-solvent, the yield and purity of the prepared product, and the creatinine content have obvious differences. When the volume ratio of the binary anti-solvent to the magnesium creatine chelate filtration solution is 0.5:1, and the volume ratio of ethanol to acetone is 2.5:1, the yield and purity of the prepared product are significantly improved, and the creatinine content is significantly reduced. Through the synergistic effect of the binary anti-solvent of ethanol and acetone, the precipitation efficiency can be improved, the solubility of the chelate can be more effectively reduced, the precipitation can be promoted, the yield can be improved, the selective precipitation of the target chelate can be facilitated, the co-precipitation of impurities can be reduced, and the amount of single anti-solvent can be reduced, thereby reducing the cost.

[0064] The product of the above experimental example 4 was detected, and the results are as follows:

[0065] Experimental formula: C4H7N3O2Mg·4H2O, which is a coordination of Mg by carboxyl and guanidyl of creatine in O, N bidentate manner 2+ , containing four molecules of fixed crystal water, forming an octahedron, XRD peak position ± 0.2°, sharp diffraction peak: 10.5°, 15.2°, 21.8°, 25.0°, 30.1° 2θ.

[0066] The yield of the magnesium creatine chelate can reach ≥85%, the HPLC purity is ≥98%, and the creatinine content is ≤0.2%.

[0067] The second group of experiments: explore the filtrate cooling to different temperatures on the yield, purity and creatinine content of the product

[0068] The difference between experimental example 4 is that: after the reaction is completed, the filtrate is cooled to 5℃, 10℃, 15℃ respectively, and the binary anti-solvent is added, and the other steps are the same as experimental example 4.

[0069] The product mass is weighed, the yield is calculated (based on the molecular weight of magnesium creatine chelate tetrahydrate 225.51g / mol), and the purity and creatinine content of the magnesium creatine chelate are tested by high performance liquid chromatograph, and the results are shown in the following table:

[0070] Table 3 Comparison of the effects of different filtrate temperatures on the yield, purity and creatinine content of the product

[0071] Experimental Example Filtrate temperature Product mass (g) Yield (%) Purity (%) Creatinine (%) Experimental Example 5 5℃ 20.30 90.0 98.8 0.06 Experimental Example 6 10℃ 19.96 88.5 98.5 0.09 Experimental Example 7 15℃ 18.94 84.0 98.1 0.14

[0072] As can be seen from the above table, when other raw materials are the same and the preparation method is consistent, changing the cooling temperature of the filtrate, the yield and purity of the product, and the creatinine content have obvious differences. When the filtrate is cooled to 5℃ and the binary anti-solvent is added, the yield and purity of the product are significantly improved, and the creatinine content is significantly reduced. At lower temperatures, the denaturation of proteins in organic solvents is smaller, so it is easier to form stable precipitates. Experiments show that when using ethanol and acetone binary anti-solvent to precipitate magnesium creatine chelate, controlling appropriate low temperature conditions helps to improve the precipitation efficiency.

[0073] The third group of experiments: explore the effects of different concentrations of creatine and magnesium salt on the yield and purity of the product, and the creatinine content

[0074] The difference between experimental example 4 is that: 14.915 grams (0.1 mole) of creatine monohydrate is weighed and dissolved in 200 milliliters, 333 milliliters, and 1000 milliliters of deionized water respectively to prepare 0.1 mol / L, 0.3 mol / L, and 0.5 mol / L creatine solutions. 20.33 grams (0.1 mole) of magnesium chloride hexahydrate is dissolved in 200 milliliters, 333 milliliters, and 1000 milliliters of deionized water respectively to prepare 0.1 mol / L, 0.3 mol / L, and 0.5 mol / L magnesium salt solutions. The other steps are the same as experimental example 4.

[0075] The product mass is weighed, the yield is calculated (based on the molecular weight of magnesium creatine chelate tetrahydrate 225.51g / mol), and the purity and creatinine content of the magnesium creatine chelate are tested by high performance liquid chromatograph, and the results are shown in the following table:

[0076] Table 4 Comparison table of product yield, purity and creatinine content at different concentrations of creatine and magnesium salt

[0077]

[0078] As can be seen from the above table, the yield is highest when the concentration of creatine is 0.3 mol / L, and the purity and creatinine content are moderate, therefore, the preferred concentration for industrialization is 0.30 mol / L. In the chelation reaction, increasing the concentration of reactants may shift the equilibrium towards the formation of chelates, thereby to some extent facilitating the formation and stability of chelates. However, if the concentration is too high, it may lead to an increase in ionic strength in the solution and an increase in the interaction between ions, which in turn affects the chelation reaction and the stability of chelates. For example, in the presence of high concentrations of metal ions and chelating agents, side reactions may occur, generating other complexes and reducing the stability of chelates.

[0079] Fourth group of experiments: explore the effect of different magnesium sources on the yield, purity and creatinine content of the product

[0080] The difference between Example 4 and this experiment is that 14.915 grams (0.1 mole) of MgCl2·6H2O, 21.445 grams (0.1 mole) of Mg(OAc)2·4H2O and 24.647 grams (0.1 mole) of MgSO4·7H2O were weighed respectively, dissolved in 200 milliliters of deionized water to prepare 0.5 mol / L different magnesium salt solutions, and the other steps were the same as those of Example 4.

[0081] The mass of the product was weighed to calculate the yield (based on the molecular weight of creatine magnesium chelate tetrahydrate 225.51 g / mol), and the purity and creatinine content of creatine magnesium chelate were tested by high performance liquid chromatograph, and the results are shown in the table below:

[0082] Table 5 Comparison table of product yield, purity and creatinine content under different magnesium sources

[0083] Experimental Example Magnesium source Product mass (g) Yield (%) Purity (%) Creatinine (%) Experimental Example 11 MgCl2.6H2O 20.30 90.0 98.8 0.06 Experimental Example 12 Mg(OAc)2.4H2O 19.98 88.6 98.2 0.07 Experimental Example 13 MgSO4.7H2O 19.87 88.1 96.9 0.07

[0084] As can be seen from the above table, when other raw materials are unchanged and the preparation process is the same, the product yield, purity and creatinine content prepared by using different magnesium sources have obvious differences, and magnesium chloride hexahydrate (MgCl2·6H2O) is the best in terms of yield / purity.

[0085] In summary, the preparation method of the creatine magnesium chelate of the present application mainly includes the following steps: preparation of a reaction solution, mixing, chelation reaction, hot microfiltration, anti-solvent crystallization, centrifugal recovery, and vacuum drying. The water-phase low-temperature production process, i.e., low-temperature chelation reaction and low-temperature binary anti-solvent precipitation, can achieve a yield of ≥85%, a purity of ≥98%, and a creatinine content of ≤0.2%, thereby solving the technical problems of low yield, high creatinine content, and low purity in the existing production methods of creatine magnesium chelate.

[0086] Obviously, the above examples and experimental examples of the present application are only examples for clearly illustrating the present application, and are not intended to limit the embodiments of the present application. Based on the above description, other different forms of changes or variations can be made by those skilled in the art. Here, it is not necessary and also impossible to enumerate all the embodiments. Any modification, equivalent replacement, and improvement, etc. made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A method for preparing a magnesium creatine chelate, characterized in that, Includes the following steps: S10. Preparation of reaction solution: Dissolve creatine and magnesium salt in water according to the specified proportions to prepare creatine solution and magnesium salt solution; S20. Mixing: Under continuous stirring, slowly and evenly mix the magnesium salt solution and creatine solution to obtain a mixture; S30. Chelation reaction: Continue stirring the mixture until complete chelation; S40. Thermal microfiltration: The solution from step S30 is passed through a microfiltration column for thermal microfiltration to remove particulate impurities; S50. Antisolvent crystallization: Cool the filtered solution from step S40 to 5-10°C, add an antisolvent to precipitate magnesium creatine chelate, wherein the antisolvent is a binary antisolvent of ethanol and acetone; S60. Centrifugal recovery: Collect crystals by centrifugation; S70. Vacuum drying: Vacuum drying of collected products.

2. The method for preparing a magnesium creatine chelate according to claim 1, characterized in that, In step S50, the volume ratio of the binary antisolvent to the magnesium creatine chelate filtration solution is 0.4-0.6:

1.

3. The method for preparing a magnesium creatine chelate according to claim 1, characterized in that, In the antisolvent of step S50, the volume ratio of ethanol to acetone is 2.2-2.8:

1.

4. The method for preparing a magnesium creatine chelate according to claim 1, characterized in that, In step S10, the concentration of the creatine solution is 0.1 to 0.5 mol / L.

5. The method for preparing a magnesium creatine chelate according to claim 1, characterized in that, In step S10, the magnesium salt is at least one of magnesium chloride, magnesium acetate, magnesium sulfate, magnesium citrate, magnesium salicylate, magnesium dihydrogen phosphate, magnesium nitrate, magnesium oxide, magnesium carbonate, magnesium hydroxide, magnesium phosphate, and magnesium oxalate.

6. The method for preparing a magnesium creatine chelate according to claim 1, characterized in that, In step S10, the molar ratio of creatine and magnesium salt is (1:0.97–1.05).

7. A method for preparing a magnesium creatine chelate according to claim 1 or 5, characterized in that, In step S10, the magnesium salt is magnesium chloride hexahydrate.

8. The method for preparing a magnesium creatine chelate according to claim 1, characterized in that, In step S30, the chelation reaction is carried out at a temperature of 25-40°C for 2-4 hours.

9. A magnesium creatine chelate prepared by a method for preparing a magnesium creatine chelate as described in any one of claims 1 to 8.

10. The application of the creatine magnesium chelate as described in claim 9 in sports nutrition supplements and medical magnesium supplements.

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