Antagonistic bacillus compound preparation and application thereof in preventing and treating rice bakanae disease

Abstract

The invention discloses an antagonistic bacillus compound preparation and application of the antagonistic bacillus compound preparation to prevention and treatment of rice bakanae disease, and relates to the technical field of agricultural biological prevention and treatment. By combining the magnetic nanoparticles and applying the specific magnetic field, the composite preparation can be guided to gather towards the root system and the disease-prone part of the rice, and by means of the precise targeted prevention and control mode, the prevention and control effect of the composite preparation on the rice bakanae disease is remarkably improved, and the problems of agent waste and environmental pollution possibly existing in a traditional prevention and control method are reduced.

Description

Technical Field

[0001] This invention relates to the field of agricultural biological control technology, specifically to a compound preparation of antagonistic Bacillus subtilis and its application in controlling rice bakanae disease. Background Technology

[0002] As one of the world's most important food crops, rice's yield and quality are directly related to food security. However, rice is susceptible to a variety of diseases during its growth. Among them, rice bakanae disease is a serious disease caused by pathogens that can lead to excessive vegetative growth, thin leaves, and reduced tillering. In severe cases, it can even cause crop failure, posing a serious threat to rice yield.

[0003] Traditional rice bakanae disease control techniques mainly rely on the spraying of chemical pesticides. This method has the following obvious drawbacks: On the one hand, it is often difficult to accurately control the distribution of chemical pesticides, resulting in some pesticides failing to effectively act on the diseased parts, causing waste of pesticides and reducing the control effect. On the other hand, the large-scale use of chemical pesticides will lead to soil, water and air pollution, disrupt the ecological balance, and pose a potential threat to the environment and human health.

[0004] Given the many shortcomings of traditional rice bakanae disease control techniques, the development of antagonistic Bacillus compound preparations and their application in controlling rice bakanae disease is of particular importance. Summary of the Invention

[0005] The purpose of this invention is to overcome the shortcomings of the prior art and provide a compound preparation of antagonistic Bacillus subtilis and its application in the prevention and control of rice bakanae disease. It can achieve precise targeted prevention and control of rice bakanae disease by combining the effects of magnetic nanoparticles and a specific magnetic field. This method not only improves the prevention and control effect and reduces pesticide waste and environmental pollution, but also reduces the risk of pathogens developing drug resistance and improves the quality and safety of agricultural products.

[0006] To solve the above-mentioned technical problems, the present invention provides the following technical solution: On one hand, an antagonistic Bacillus compound preparation is made from the following raw materials in parts by weight:

[0007] The compound formulation contains antagonistic Bacillus subtilis and magnetic nanoparticles.

[0008] Furthermore, the antagonistic Bacillus is a Bacillus strain that has a significant inhibitory effect on the pathogen of rice bakanae disease, including but not limited to Bacillus subtilis and Bacillus amyloliquefaciens.

[0009] Furthermore, the magnetic nanoparticles are iron(III) oxide nanoparticles or iron(II) oxide nanoparticles.

[0010] Furthermore, the magnetic nanoparticles have a particle size range of 10-100 nm.

[0011] Furthermore, the mass ratio of the antagonistic Bacillus to the magnetic nanoparticles is (1-10):1.

[0012] On the other hand, the application of the antagonistic Bacillus compound preparation in the prevention and control of rice bakanae disease is characterized in that, during the rice planting process, the antagonistic Bacillus compound preparation is applied to the paddy field, and at the same time, a specific magnetic field is applied in the paddy field. The magnetic induction intensity of the specific magnetic field is 0.1-1T, and the direction of the magnetic field is perpendicular to the plane of the paddy field. The compound preparation is guided to accumulate in the rice root system and disease-prone parts through the action of the magnetic field, so as to achieve precise targeted prevention and control of rice bakanae disease.

[0013] Furthermore, the method of applying the antagonistic Bacillus compound preparation during rice cultivation is to apply it before sowing or spray it after transplanting.

[0014] Furthermore, the application dosage of the antagonistic Bacillus compound preparation is 0.8-1.2 kg per acre.

[0015] Furthermore, when using post-transplant spraying, the application rate of the antagonistic Bacillus compound preparation is 1-1.5 kg per acre.

[0016] Furthermore, the specific magnetic field continues to act on the paddy field for 1-3 hours.

[0017] Compared with existing technologies, this antagonistic Bacillus compound preparation and its application in the control of rice bakanae disease have the following beneficial effects:

[0018] First, this antagonistic Bacillus compound preparation, by combining magnetic nanoparticles and applying a specific magnetic field, can guide the compound preparation to accumulate in the rice root system and disease-prone parts. This precise and targeted control method significantly improves the control effect of the compound preparation on rice bakanae disease and reduces the waste of pesticides and environmental pollution that may exist in traditional control methods.

[0019] Second, this antagonistic Bacillus compound preparation utilizes the principle of biological control. By antagonizing Bacillus, it significantly inhibits the pathogen of rice bakanae disease, reducing dependence on chemical pesticides. This not only helps protect the ecological environment and reduce pesticide residue pollution of agricultural products and soil, but also improves the quality and safety of rice, meeting the requirements of sustainable development in modern agriculture.

[0020] Other advantages, objectives and features of the invention will be set forth in part in the description which follows, and in part will be apparent to those skilled in the art from the following examination or study, or may be learned from the practice of the invention. Attached Figure Description

[0021] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the accompanying drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are merely some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without any creative effort.

[0022] Figure 1 A flowchart illustrating the application of a compound preparation for antagonizing Bacillus subtilis and its use in controlling rice bakanae disease. Detailed Implementation

[0023] To further illustrate the technical means and effects of the present invention in achieving its intended purpose, the following detailed description of the specific implementation methods, structures, features, and effects of the present invention, in conjunction with the accompanying drawings and preferred embodiments, is provided below.

[0024] Examples 1 to 3 only varied the mass ratio of antagonistic Bacillus to magnetic nanoparticles.

[0025] Example 1

[0026] Antagonistic Bacillus: Bacillus subtilis, which has a significant inhibitory effect on the pathogen of rice bakanae disease, was selected. After laboratory screening and culture, it showed good activity.

[0027] Magnetic nanoparticles: Iron oxide nanoparticles with a particle size of 50 nm are used to ensure their dispersibility and magnetic responsiveness in subsequent operations.

[0028] The preparation was carried out according to the mass ratio of antagonistic Bacillus subtilis to magnetic nanoparticles of 2:1. First, a quantitative Bacillus subtilis culture was pretreated by centrifugation and washing to remove impurities and excess culture medium components. Then, the treated Bacillus subtilis and iron oxide nanoparticles were thoroughly mixed in a sterile environment and gently stirred to make them uniformly dispersed, finally obtaining the antagonistic Bacillus subtilis composite preparation.

[0029] Application method: Apply by spraying after rice transplanting.

[0030] Application dosage: Weigh the corresponding weight of the antagonistic Bacillus compound preparation according to the standard of 1.2 kg per mu, dissolve it in an appropriate amount of water, and prepare a uniform spray solution.

[0031] Magnetic field setup: Three days after rice transplanting, a specific magnetic field is set up in the paddy field. The magnetic induction intensity of the magnetic field is adjusted to 0.5T, and the direction of the magnetic field is perpendicular to the plane of the paddy field to ensure that the magnetic field evenly covers the entire paddy field area.

[0032] Operation process: Using professional spraying equipment, the prepared antagonistic Bacillus compound preparation solution is evenly sprayed onto the rice plants and the surface of the paddy field. During the spraying process, the magnetic field equipment is turned on at the same time, so that the specific magnetic field continues to act in the paddy field for 2 hours.

[0033] Results: After treatment in this embodiment, among the 500 rice plants investigated, the number of diseased plants was 45, the incidence rate was reduced to 9%, and the disease index was 12.5. Compared with the control group, the control efficacy reached 71.6%. At the same time, the growth of rice was improved, with an average plant height of 82.3 cm, root length of 11.5 cm, and thousand-grain weight of 27.3 g, all of which were better than the untreated control group.

[0034] Example 2

[0035] Antagonistic Bacillus: Bacillus subtilis, which has a significant inhibitory effect on the pathogen of rice bakanae disease, was selected. After laboratory screening and culture, it showed good activity.

[0036] Magnetic nanoparticles: Iron oxide nanoparticles with a particle size of 50 nm are used to ensure their dispersibility and magnetic responsiveness in subsequent operations.

[0037] The preparation was carried out according to the mass ratio of antagonistic Bacillus subtilis to magnetic nanoparticles of 8:1. First, a quantitative Bacillus subtilis culture was pretreated by centrifugation and washing to remove impurities and excess culture medium components. Then, the treated Bacillus subtilis and iron oxide nanoparticles were thoroughly mixed in a sterile environment and uniformly dispersed by gentle stirring to finally obtain the antagonistic Bacillus subtilis composite preparation.

[0038] Application method: Apply by spraying after rice transplanting.

[0039] Application dosage: Weigh the corresponding weight of the antagonistic Bacillus compound preparation according to the standard of 1.2 kg per mu, dissolve it in an appropriate amount of water, and prepare a uniform spray solution.

[0040] Magnetic field setup: Three days after rice transplanting, a specific magnetic field is set up in the paddy field. The magnetic induction intensity of the magnetic field is adjusted to 0.5T, and the direction of the magnetic field is perpendicular to the plane of the paddy field to ensure that the magnetic field evenly covers the entire paddy field area.

[0041] Operation process: Using professional spraying equipment, the prepared antagonistic Bacillus compound preparation solution is evenly sprayed onto the rice plants and the surface of the paddy field. During the spraying process, the magnetic field equipment is turned on at the same time, so that the specific magnetic field continues to act in the paddy field for 2 hours.

[0042] Results: After treatment in this example, in the same survey sample of 500 rice plants, only 18 plants were diseased, the incidence rate was as low as 3.6%, the disease index was 6.8, the control efficacy was increased to 89.2%, and the rice growth performance was better, with the average plant height increasing to 86.5cm, the root length reaching 13.2cm, and the thousand-grain weight increasing to 29.1g. All growth indicators were significantly better than the control group.

[0043] Example 3

[0044] Antagonistic Bacillus: Bacillus subtilis, which has a significant inhibitory effect on the pathogen of rice bakanae disease, was selected. After laboratory screening and culture, it showed good activity.

[0045] Magnetic nanoparticles: Iron oxide nanoparticles with a particle size of 50 nm are used to ensure their dispersibility and magnetic responsiveness in subsequent operations.

[0046] The preparation was carried out according to the mass ratio of antagonistic Bacillus subtilis to magnetic nanoparticles of 10:1. First, a quantitative Bacillus subtilis culture was pretreated by centrifugation and washing to remove impurities and excess culture medium components. Then, the treated Bacillus subtilis and iron oxide nanoparticles were thoroughly mixed in a sterile environment and gently stirred to make them uniformly dispersed, finally obtaining the antagonistic Bacillus subtilis composite preparation.

[0047] Application method: Apply by spraying after rice transplanting.

[0048] Application dosage: Weigh the corresponding weight of the antagonistic Bacillus compound preparation according to the standard of 1.2 kg per mu, dissolve it in an appropriate amount of water, and prepare a uniform spray solution.

[0049] Magnetic field setup: Three days after rice transplanting, a specific magnetic field is set up in the paddy field. The magnetic induction intensity of the magnetic field is adjusted to 0.5T, and the direction of the magnetic field is perpendicular to the plane of the paddy field to ensure that the magnetic field evenly covers the entire paddy field area.

[0050] Operation process: Using professional spraying equipment, the prepared antagonistic Bacillus compound preparation solution is evenly sprayed onto the rice plants and the surface of the paddy field. During the spraying process, the magnetic field equipment is turned on at the same time, so that the specific magnetic field continues to act in the paddy field for 2 hours.

[0051] Results: In this example, only 15 out of 500 rice plants were infected, further reducing the disease incidence rate to 3%, with a disease index of 6.2 and a control efficacy of up to 91%. The rice plants grew well, with an average plant height of 87.2 cm, root length of 13.8 cm, and thousand-grain weight of 29.5 g. Both the disease control effect and the promotion of rice growth were significantly better than the control group and the previous two examples.

[0052] Examples four to six only change specific magnetic fields

[0053] Example 4

[0054] Antagonistic Bacillus: Bacillus subtilis, which has a significant inhibitory effect on the pathogen of rice bakanae disease, was selected. After laboratory screening and culture, it showed good activity.

[0055] Magnetic nanoparticles: Iron oxide nanoparticles with a particle size of 50 nm are used to ensure their dispersibility and magnetic responsiveness in subsequent operations.

[0056] The preparation was carried out according to the mass ratio of antagonistic Bacillus subtilis to magnetic nanoparticles of 5:1. First, a quantitative Bacillus subtilis culture was pretreated by centrifugation and washing to remove impurities and excess culture medium components. Then, the treated Bacillus subtilis and iron oxide nanoparticles were thoroughly mixed in a sterile environment and gently stirred to make them uniformly dispersed, finally obtaining the antagonistic Bacillus subtilis composite preparation.

[0057] Application method: Apply by spraying after rice transplanting.

[0058] Application dosage: Weigh the corresponding weight of the antagonistic Bacillus compound preparation according to the standard of 1.2 kg per mu, dissolve it in an appropriate amount of water, and prepare a uniform spray solution.

[0059] Magnetic field setup: Three days after rice transplanting, a specific magnetic field is set up in the paddy field. The magnetic induction intensity of the magnetic field is adjusted to 0.1T, and the direction of the magnetic field is perpendicular to the plane of the paddy field to ensure that the magnetic field evenly covers the entire paddy field area.

[0060] Operation process: Using professional spraying equipment, the prepared antagonistic Bacillus compound preparation solution is evenly sprayed onto the rice plants and the surface of the paddy field. During the spraying process, the magnetic field equipment is turned on at the same time, so that the specific magnetic field continues to act in the paddy field for 2 hours.

[0061] Results: After treatment in this example, among the 500 rice plants surveyed, 75 plants were infected, the incidence rate dropped to 15%, and the disease index was 18.3. Compared with the control group, the control efficacy reached 54.7%. The growth of rice improved, with an average plant height of 80.2 cm, root length of 10.8 cm, and thousand-grain weight of 26.5 g, all of which were better than the untreated control group.

[0062] Example 5

[0063] Antagonistic Bacillus: Bacillus subtilis, which has a significant inhibitory effect on the pathogen of rice bakanae disease, was selected. After laboratory screening and culture, it showed good activity.

[0064] Magnetic nanoparticles: Iron oxide nanoparticles with a particle size of 50 nm are used to ensure their dispersibility and magnetic responsiveness in subsequent operations.

[0065] The preparation was carried out according to the mass ratio of antagonistic Bacillus subtilis to magnetic nanoparticles of 5:1. First, a quantitative Bacillus subtilis culture was pretreated by centrifugation and washing to remove impurities and excess culture medium components. Then, the treated Bacillus subtilis and iron oxide nanoparticles were thoroughly mixed in a sterile environment and gently stirred to make them uniformly dispersed, finally obtaining the antagonistic Bacillus subtilis composite preparation.

[0066] Application method: Apply by spraying after rice transplanting.

[0067] Application dosage: Weigh the corresponding weight of the antagonistic Bacillus compound preparation according to the standard of 1.2 kg per mu, dissolve it in an appropriate amount of water, and prepare a uniform spray solution.

[0068] Magnetic field setup: Three days after rice transplanting, a specific magnetic field is set up in the paddy field. The magnetic induction intensity of the magnetic field is adjusted to 0.8T, and the direction of the magnetic field is perpendicular to the plane of the paddy field to ensure that the magnetic field evenly covers the entire paddy field area.

[0069] Operation process: Using professional spraying equipment, the prepared antagonistic Bacillus compound preparation solution is evenly sprayed onto the rice plants and the surface of the paddy field. During the spraying process, the magnetic field equipment is turned on at the same time, so that the specific magnetic field continues to act in the paddy field for 2 hours.

[0070] Results: After treatment in this example, in the same survey sample of 500 rice plants, only 20 plants were diseased, the incidence rate was as low as 4%, the disease index was 7.1, the control efficacy was increased to 87.7%, and the rice growth performance was better, with the average plant height increasing to 85.8cm, the root length reaching 12.9cm, and the thousand-grain weight increasing to 28.9g. All growth indicators were significantly better than the control group.

[0071] Example 6

[0072] Antagonistic Bacillus: Bacillus subtilis, which has a significant inhibitory effect on the pathogen of rice bakanae disease, was selected. After laboratory screening and culture, it showed good activity.

[0073] Magnetic nanoparticles: Iron oxide nanoparticles with a particle size of 50 nm are used to ensure their dispersibility and magnetic responsiveness in subsequent operations.

[0074] The preparation was carried out according to the mass ratio of antagonistic Bacillus subtilis to magnetic nanoparticles of 5:1. First, a quantitative Bacillus subtilis culture was pretreated by centrifugation and washing to remove impurities and excess culture medium components. Then, the treated Bacillus subtilis and iron oxide nanoparticles were thoroughly mixed in a sterile environment and gently stirred to make them uniformly dispersed, finally obtaining the antagonistic Bacillus subtilis composite preparation.

[0075] Application method: Apply by spraying after rice transplanting.

[0076] Application dosage: Weigh the corresponding weight of the antagonistic Bacillus compound preparation according to the standard of 1.2 kg per mu, dissolve it in an appropriate amount of water, and prepare a uniform spray solution.

[0077] Magnetic field setup: Three days after rice transplanting, a specific magnetic field is set up in the paddy field. The magnetic induction intensity of the magnetic field is adjusted to 1T, and the direction of the magnetic field is perpendicular to the plane of the paddy field to ensure that the magnetic field evenly covers the entire paddy field area.

[0078] Operation process: Using professional spraying equipment, the prepared antagonistic Bacillus compound preparation solution is evenly sprayed onto the rice plants and the surface of the paddy field. During the spraying process, the magnetic field equipment is turned on at the same time, so that the specific magnetic field continues to act in the paddy field for 2 hours.

[0079] Results: In this example, only 12 out of 500 rice plants were infected, further reducing the disease incidence rate to 2.4%, with a disease index of 5.8 and a control efficacy of 92.8%. The rice plants grew well, with an average plant height of 86.7 cm, root length of 13.5 cm, and thousand-grain weight of 29.3 g. Both the disease control effect and the promotion of rice growth were significantly better than the control group and the first example.

[0080] The specific content is shown in the table below:

[0081]

[0082] As shown in the table above, Examples 1 to 3, by adjusting the mass ratio of antagonistic Bacillus to magnetic nanoparticles, observed significant differences in control effects under different ratios while keeping other parameters constant. When the mass ratio was 2:1, the control efficacy against rice bakanae disease was 71.6%, the incidence rate dropped to 9%, and the improvement in plant growth indicators was relatively small. As the content of magnetic nanoparticles decreased, the control efficacy increased to 89.2% and 91% at mass ratios of 8:1 and 10:1, respectively, and the incidence rate further decreased to 3.6% and 3%. The growth indicators of rice, such as plant height, root length, and thousand-grain weight, also significantly improved. This indicates that within a certain range, appropriately reducing the content of magnetic nanoparticles and increasing the proportion of antagonistic Bacillus can improve the control effect of the compound preparation against rice bakanae disease and its promoting effect on rice growth. However, the specific ratio needs to be considered in conjunction with actual needs and costs.

[0083] Examples 4 to 6, by adjusting the magnetic induction intensity of a specific magnetic field while keeping other parameters constant, observed significant differences in the control effects of the antagonistic Bacillus compound preparation under different magnetic field intensities. When the magnetic field intensity was 0.1T, the control efficacy against rice bakanae disease was 54.7%, the incidence rate decreased to 15%, and the improvement in plant growth indicators was relatively small. As the magnetic field intensity increased to 0.8T and 1T, the control efficacy increased to 87.7% and 92.8%, respectively, and the incidence rate further decreased to 4% and 2.4%. Rice plant height, root length, and thousand-grain weight also significantly improved. This indicates that within a certain range, appropriately increasing the magnetic field intensity can enhance the guiding effect of the magnetic field on magnetic nanoparticles, promote the precise aggregation of the compound preparation to rice roots and diseased parts, thereby improving the control effect against rice bakanae disease and promoting rice growth. However, excessively high magnetic field intensity may increase equipment costs and energy consumption. In practical applications, it is necessary to comprehensively consider the control effect and economic efficiency to select appropriate magnetic field parameters.

[0084] The above description is merely a preferred embodiment of the present invention and is not intended to limit the present invention in any way. Although the present invention has been disclosed above with reference to preferred embodiments, it is not intended to limit the present invention. Any person skilled in the art can make some modifications or alterations to the above-disclosed technical content to create equivalent embodiments without departing from the scope of the present invention. Any simple modifications, equivalent changes and alterations made to the above embodiments based on the technical essence of the present invention without departing from the scope of the present invention shall still fall within the scope of the present invention.

Claims

1. A compound preparation antagonizing Bacillus subtilis, characterized in that, Made from the following raw materials in parts by weight: The compound formulation contains antagonistic Bacillus subtilis and magnetic nanoparticles.

2. The antagonistic Bacillus compound preparation according to claim 1, characterized in that, The antagonistic Bacillus strains are Bacillus strains that have a significant inhibitory effect on the pathogen of rice bakanae disease, including but not limited to Bacillus subtilis and Bacillus amyloliquefaciens.

3. The antagonistic Bacillus compound preparation according to claim 1, characterized in that, The magnetic nanoparticles are iron(III) oxide nanoparticles or iron(II) oxide nanoparticles.

4. The antagonistic Bacillus compound preparation according to claim 1, characterized in that, The magnetic nanoparticles have a particle size range of 10-100 nm.

5. The antagonistic Bacillus compound preparation according to claim 1, characterized in that, The mass ratio of the antagonistic Bacillus to the magnetic nanoparticles is (1-10):

1.

6. The application of a compound preparation of antagonistic Bacillus subtilis according to any one of claims 1-5 in the prevention and control of rice bakanae disease, characterized in that, The method includes the following steps: During rice planting, an antagonistic Bacillus compound preparation is applied to the paddy field, and a specific magnetic field is applied in the paddy field. The magnetic induction intensity of the specific magnetic field is 0.1-1T, and the direction of the magnetic field is perpendicular to the plane of the paddy field. The magnetic field guides the compound preparation to accumulate in the rice root system and disease-prone parts, thereby achieving precise targeted prevention and control of rice bakanae disease.

7. The antagonistic Bacillus compound preparation according to claim 6, characterized in that, The method of applying the antagonistic Bacillus compound preparation during rice planting is to apply it before sowing or spray it after transplanting.

8. The antagonistic Bacillus compound preparation according to claim 6, characterized in that, The application dosage of the antagonistic Bacillus compound preparation is 0.8-1.2 kg per mu.

9. The antagonistic Bacillus compound preparation according to claim 6, characterized in that, When using post-transplant spraying, the dosage of the antagonistic Bacillus compound preparation is 1-1.5 kg per acre.

10. The antagonistic Bacillus compound preparation according to claim 6, characterized in that, The specific magnetic field lasts for 1-3 hours in the paddy field.

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