Preparation process for efficiently absorbing fish protein peptide and application of fish protein peptide in fertilizer

Abstract

The invention discloses a preparation process of high-efficiency absorption fish protein peptide and application of the high-efficiency absorption fish protein peptide in fertilizer, and belongs to the technical field of marine organisms. The aquatic product protein is directly used as a fermentation raw material, and the content of amino acid in the fish protein biological peptide is remarkably increased by utilizing the synergistic fermentation effect of microorganisms and assisted by biological enzymolysis, so that the absorptivity of the fish protein peptide is increased; the prepared fish protein peptide is used in the fertilizer to prepare the fish protein peptide functional fertilizer, the nutritional value and the utilization rate of the fertilizer are remarkably improved, and the fish protein peptide functional fertilizer has the remarkable effects of growth promotion, disease resistance, stress resistance and the like. According to the method, a conventional fish protein peptide preparation method of firstly preparing fermentation enzyme liquid and then performing biological enzymolysis is abandoned, the efficient absorption fish protein peptide is prepared by adopting a process of directly fermenting aquatic protein, a fermentation process and an enzymolysis process are integrated, and compared with a conventional process, the method has the advantages of simple production process, low production cost and the like; the method has significant effects and meanings for promoting green preparation of the fish protein peptide and efficient application of the fish protein peptide in fertilizers.

Description

TECHNICAL FIELD

[0001] The present application relates to a preparation process of high-efficiency fish protein peptide and its application in fertilizer, in particular to a high-amino-acid-content fish protein biological peptide prepared by microbial fermentation method, and its application in the preparation of biological organic fertilizer, belonging to the field of marine biotechnology. BACKGROUND

[0002] Fish protein peptide fertilizer is rich in various amino acids, small peptides, vitamins, trace elements (such as calcium, magnesium, zinc, iron, etc.) and bioactive substances (such as polysaccharides, polyphenols, taurine, etc.). Among them, amino acids and small peptides can be directly absorbed and utilized by crops without going through complex conversion process, providing nutrients for crops quickly, so the fish protein peptide fertilizer is developing rapidly at present. In recent years, research on fish protein peptide fertilizer has become a hot spot in this technical field, such as patent 2004100356188 discloses a fish protein biological peptide fertilizer and its preparation process, the fertilizer is rich in biological peptides and trace elements such as calcium, phosphorus and zinc, which can effectively improve the disease resistance and stress resistance of plants, thereby improving the quality of crops and promoting plant growth; patent 2024104284171 discloses a high-efficiency fish protein fertilizer and its preparation process, the nutrient substances in the fish protein fertilizer are slowly released for plant growth, the free metal elements in the soil are utilized, and the root system is protected from diseases and pests.

[0003] Protein peptide is a general term for different peptides of linear and ring structure from dipeptide composed of 20 natural amino acids in different compositions and arrangements. Studies have shown that low molecular weight peptides are digested faster and absorbed more than free amino acids, and have higher biological potency and nutritional value than free amino acids; and small peptides produced by protein enzymolysis have the effects of easy digestion, absorption, promotion of calcium, phosphorus and other trace element absorption, enhancement of macrophage and B cell activity, enhancement of immune function, protection of epidermal cells, etc. Fish protein peptide is a kind of aquatic protein peptide, which is prepared by autolysis, enzymolysis, liquid fermentation, or solid-state fermentation combined with liquid enzymolysis, etc. using fish tissue as material, among which fermentation or enzymolysis is the most commonly used method. For example, patent 2024107361589 discloses an extraction equipment and method of fish protein peptide, patent 2016109805255 discloses a preparation method of fish protein peptide and fish protein peptide obtained thereby, and patent 2021113311418 discloses a fish protein peptide and its preparation method, all of which use protease enzymolysis process to prepare fish protein peptide.

[0004] The analysis of the prior art can find that the current fish protein peptide extraction mainly adopts enzymatic method or microbial fermentation method, wherein the enzymatic method is mainly adopted. Although the fish protein peptide fertilizer prepared by the enzymatic method has obtained many developments, the improvement of the amino acid content in the fish protein peptide fertilizer mainly depends on the protease preparation. Since the raw material for preparing the fish protein peptide fertilizer is mixed, higher requirements are put forward for the protease, which restricts the play of the nutritional function of the fish protein peptide fertilizer and greatly increases the cost of the fish protein peptide preparation. The fish protein peptide fertilizer still has the technical defects of low amino acid content, complex production process, high production cost and the like, and it is urgent to develop a fish protein peptide preparation process with simple production process, low production cost, high polypeptide amino acid content and high efficiency and easy absorption. SUMMARY

[0005] In order to overcome the defects of the prior art, the purpose of the present application is to provide a preparation process of high-efficiency absorption fish protein peptide and application thereof in fertilizer, utilize the synergistic fermentation of microorganisms, supplemented by biological enzymolysis, significantly improve the amino acid content in the fish protein biological peptide, further develop the fish protein biological peptide organic fertilizer, and then improve the nutritional property of the biological organic fertilizer, improve the disease resistance and stress resistance of plants, and promote the growth of plants, so as to provide a high-efficiency and novel animal protein fertilizer, and realize the high-efficiency application of the fish protein biological peptide in fertilizer.

[0006] The above purpose of the present application is realized by the following technical scheme: The preparation process of high-efficiency absorption fish protein peptide comprises the following steps: (1) grinding and stirring after mixing the aquatic animal protein and water to obtain a mixed slurry, and adjusting the pH value to be higher than 7.0; (2) adding a compound fermentation strain to the mixed slurry and stirring uniformly, the addition amount of the compound fermentation strain is 3% to 5% of the mixed slurry, the fermentation temperature is 40 to 60 DEG C, and the fermentation time is 24 to 36 h; (3) after the fermentation is completed, the fermentation liquid is subjected to solid-liquid separation, the solid matter in the fermentation liquid is removed through a filtration or centrifugal process, and a fish protein polypeptide liquid is obtained; (4) concentrating the fish protein polypeptide liquid, and concentrating to more than 20% of solid matter, so as to obtain the fish protein peptide.

[0007] Preferably, the mixing ratio of the aquatic animal protein and water in step (1) is 1:1.5 to 2.0.

[0008] Preferably, 5% of soybean meal, 0.2% of Na2HPO4, 7 kg of KH2PO4 and 0.03% of CaCl2 are added to the mixed slurry in step (1).

[0009] Preferably, the compound fermentation bacteria in step (2) is one of Pseudomonas alternanivorans SM9913 and Bacillus DFH-1 or a mixed bacteria of the two.

[0010] The Pseudomonas alternanivorans SM9913 in the fermentation bacteria can be obtained by market purchase; the Bacillus DFH-1 is provided by Qingdao Haide Group Co., Ltd., and the bacteria strain preservation number is CGMCC No. 30326.

[0011] Preferably, the compound fermentation bacteria in step (2) is a mixed bacteria of Pseudomonas alternanivorans SM9913 and Bacillus DFH-1, and the composition ratio is 3:1.

[0012] Preferably, 0.1% to 0.5% of a compound protease is added in step (2), and the compound protease is a mixture of one or more of trypsin, pepsin and papain.

[0013] The activation culture of Pseudomonas alternanivorans SM9913 and Bacillus DFH-1 in the application is prepared from 1% of proteose peptone and 0.5% of yeast powder in natural seawater, and the pH is 8.0, and the high-pressure steam sterilization is performed at 121°C for 20 min.

[0014] Preferably, the fish protein peptide powder is prepared by a spray drying or freeze drying process. Preferably, the spray drying process parameters are as follows: the inlet air temperature is 200°C, and the outlet air temperature is 100°C.

[0015] The application provides application of high-efficiency fish protein peptide in fertilizers, and the fish protein peptide is in the form of powder or liquid, and can be compounded with nitrogen, phosphorus and potassium, trace elements, microbial agents and the like to form different functional foliar fertilizers, flush fertilizers, microbial fertilizers, biological organic fertilizers and organic-inorganic compound fertilizers.

[0016] The application provides a preparation process of high-efficiency fish protein peptide and application technology of the fish protein peptide in fertilizers. (1) The application uses low-value aquatic protein (aquatic processing waste, fish skin and the like) as raw material, directly uses the raw material for fermentation, utilizes the synergistic fermentation of microorganisms, and supplements biological enzymolysis, so that the amino acid content in the fish protein biological peptide is significantly improved, and the absorption rate of the fish protein peptide is improved; the prepared fish protein peptide is used in fertilizers to prepare fish protein peptide functional fertilizers, the nutritional value of the fish protein peptide fertilizer is significantly improved, and the fish protein peptide functional fertilizer has the effects of significantly promoting growth, resisting diseases and resisting stresses. (2) The present application discards the previous preparation of fermentation enzyme liquid and then carries out biological enzymatic hydrolysis fish protein peptide preparation method, adopts the process of direct fermentation of aquatic protein to prepare high-efficiency absorption fish protein peptide, and the fermentation process and the enzymolysis process are combined, compared with the traditional process, has the advantages of simple production process, low production cost and the like, and has remarkable effect and significance for promoting the green preparation of fish protein peptide and its efficient application in fertilizer; (3) The present application utilizes microbial compound fermentation to degrade fish skin protein, and through the synergistic effect between the microbial fermentation strains, the amino acid content in the fish protein peptide is significantly improved, and the experimental results show that the synergistic effect between the microbial strains SM9913 and Bacillus DFH-1 achieves a technical effect of “1+1>2”, the polypeptide amino acid content in the fish protein peptide is high, efficient and easy to absorb, and the application value in the fertilizer is significantly improved; meanwhile, a certain amount of compound protein is added in the fermentation liquid, so that the amino acid content in the fish protein peptide can be further improved; (4) The present application utilizes microbial direct fermentation to degrade fish protein, and a large amount of extracellular polysaccharide is produced in the fermentation process, which has a significant effect on subsequent concentration, drying and the like, and reduces the concentration and drying cost; the produced microbial polysaccharide has a significant synergistic effect on improving the application value of the fish protein peptide in the fertilizer; compared with the process of preparing enzyme liquid by fermentation and then degrading fish protein, the microbial polysaccharide content in the protein peptide of the present application is significantly improved, and a significant technical effect is achieved; (5) The present application provides a preparation process of high-efficiency absorption fish protein peptide and its reserved energy use in fertilizer, the fish protein peptide prepared by the method has been applied to actual production, and commercial success has been achieved, at present, the enterprise standard Q / 370202WXS 093-2025 “amino acid-containing water-soluble fertilizer” has been filed, and it is used for the production of fertilizer, and remarkable economic benefits have been achieved. BRIEF DESCRIPTION OF DRAWINGS

[0017] Figure 1 It is a contrast chart of cucumber rooting after the first fertilization; Figure 2 It is a contrast chart of the longest root length of cucumber after the second fertilization; Figure 3 It is a contrast chart of the total root length of cucumber after the second fertilization; Figure 4 It is a contrast chart of the root number of cucumber after the second fertilization. DETAILED DESCRIPTION

[0018] The activation culture medium of the microbial fermentation strains SM9913 and Bacillus DFH-1 used in the following examples is as follows: 1% proteose peptone, 0.5% yeast powder, natural seawater, pH 8.0, 121ºC high-pressure steam sterilization for 20 min. EMBODIMENT

[0019] A preparation process of fish protein peptide with high absorption efficiency, specifically comprising the following steps: (1) After mixing aquatic animal protein with water, grinding and stirring are performed to obtain a mixed slurry, and the pH value is adjusted to be higher than 7.0, wherein the mixing ratio of aquatic animal protein to water is 1:1.8, and 5% soybean meal, 0.2% Na2HPO4 7 kg, 0.01% KH2PO4 and 0.03% CaCl2 are added to the slurry; (2) A compound fermentation strain is added to the mixed slurry and stirred uniformly, the addition amount of the compound fermentation strain is 3% of the mixed slurry, the fermentation temperature is 40-60°C, and the fermentation time is 36h; the compound fermentation strain is a mixed strain of Pseudoalteromonas sp. SM9913 and Bacillus sp. DFH-1, and the matching ratio is 3:1; (3) After the fermentation is completed, the fermentation liquor is subjected to solid-liquid separation, and the solid content of the fermentation liquor is removed through a centrifugal process to obtain a fish protein polypeptide liquor; (4) The fish protein polypeptide liquor is concentrated to a solid content of more than 20% to obtain a fish protein peptide liquid; (5) The concentrated fish protein peptide liquid is subjected to spray drying to prepare a fish protein peptide powder preparation. Embodiment

[0020] A preparation process of fish protein peptide with high absorption efficiency, specifically comprising the following steps: (1) After mixing aquatic animal protein with water, grinding and stirring are performed to obtain a mixed slurry, and the pH value is adjusted to be higher than 7.0, wherein the mixing ratio of aquatic animal protein to water is 1:1.8, and 5% soybean meal, 0.2% Na2HPO4 7 kg, 0.01% KH2PO4 and 0.03% CaCl2 are added to the slurry; (2) A compound fermentation strain Pseudoalteromonas sp. SM9913 is added to the mixed slurry and stirred uniformly, the addition amount of the strain is 3% of the mixed slurry, the fermentation temperature is 40-60°C, and the fermentation time is 36h; (3) After the fermentation is completed, the fermentation liquor is subjected to solid-liquid separation, and the solid content of the fermentation liquor is removed through a centrifugal process to obtain a fish protein polypeptide liquor; (4) The fish protein polypeptide liquor is concentrated to a solid content of more than 20% to obtain a fish protein peptide liquid; (5) The concentrated fish protein peptide liquid is subjected to spray drying to prepare a fish protein peptide powder preparation. Embodiment

[0021] A preparation process of fish protein peptide with high absorption efficiency, specifically comprising the following steps: (1) grind and stir the aquatic animal protein mixed with water to obtain a mixed slurry, adjust the pH value to be above 7.0, wherein the mixing ratio of aquatic animal protein to water is 1:1.8, and 5% soybean meal, 0.2% Na2HPO4 7 kg, 0.01% KH2PO4, and 0.03% CaCl2 are added to the slurry; (2) add the fermentation strain Bacillus DFH-1 to the mixed slurry and stir evenly, the addition amount of the fermentation strain is 3% of the mixed slurry, the fermentation temperature is 40-60°C, and the fermentation time is 36h; (3) after the fermentation is completed, the fermentation liquid is subjected to solid-liquid separation, and the solid content of the fermentation liquid is removed by centrifugation to obtain a fish protein polypeptide liquid; (4) the fish protein polypeptide liquid is concentrated to a solid content of more than 20% to obtain a fish protein peptide liquid; (5) the concentrated fish protein peptide liquid is spray dried to prepare a fish protein peptide powder preparation. Embodiment

[0022] A preparation process of fish protein peptide with high absorption efficiency, specifically comprising the following steps: (1) grind and stir the aquatic animal protein mixed with water to obtain a mixed slurry, adjust the pH value to be above 7.0, wherein the mixing ratio of aquatic animal protein to water is 1:1.8, and 5% soybean meal, 0.2% Na2HPO4 7 kg, 0.01% KH2PO4, and 0.03% CaCl2 are added to the slurry; (2) add the compound fermentation strain to the mixed slurry and stir evenly, the addition amount of the compound fermentation strain is 3% of the mixed slurry, the fermentation temperature is 40-60°C, and the fermentation time is 36h; the compound fermentation strain is a mixed strain of Pseudoalteromonas SM9913 and Bacillus DFH-1, and the matching ratio is 1:1; (3) after the fermentation is completed, the fermentation liquid is subjected to solid-liquid separation, and the solid content of the fermentation liquid is removed by centrifugation to obtain a fish protein polypeptide liquid; (4) the fish protein polypeptide liquid is concentrated to a solid content of more than 20% to obtain a fish protein peptide liquid; (5) the concentrated fish protein peptide liquid is spray dried to prepare a fish protein peptide powder preparation. Embodiment

[0023] A preparation process of fish protein peptide with high absorption efficiency, specifically comprising the following steps: (1) The aquatic animal protein is mixed with water and then ground and stirred to obtain a mixed slurry, and the pH value is adjusted to be higher than 7.0, wherein the mixing ratio of the aquatic animal protein to water is 1:1.8, and 5% soybean meal, 0.2% Na2HPO4, 7 kg, 0.01% KH2PO4, and 0.03% CaCl2 are added to the slurry; (2) A compound fermentation strain is added to the mixed slurry and stirred uniformly, the addition amount of the compound fermentation strain is 3% of the mixed slurry, the fermentation temperature is 40-60°C, and the fermentation time is 36 h; the compound fermentation strain is a mixed strain of Pseudoalteromonas SM9913 and Bacillus DFH-1, and the matching ratio is 3:1; at the same time, 0.2% of a compound protease is added, the protease is a mixture of trypsin and papain, and the composition ratio of trypsin to papain is 3:2; (3) After the fermentation is completed, the fermentation liquid is subjected to solid-liquid separation, and the solid matter in the fermentation liquid is removed through a centrifugal process to obtain a fish protein polypeptide liquid; (4) The fish protein polypeptide liquid is concentrated to a solid matter content of more than 20% to obtain a fish protein peptide liquid; (5) The concentrated fish protein peptide liquid is subjected to spray drying to prepare a fish protein peptide powder preparation.

[0024] A preparation process of fish protein peptides with high absorption efficiency, specifically comprising the following steps: (1) The aquatic animal protein is mixed with water and then ground and stirred to obtain a mixed slurry, and the pH value is adjusted to be higher than 7.0, wherein the mixing ratio of the aquatic animal protein to water is 1:1.8, and 5% soybean meal, 0.2% Na2HPO4, 7 kg, 0.01% KH2PO4, and 0.03% CaCl2 are added to the slurry; (2) 0.2% of a compound protease is added to the mixed slurry, the protease is a mixture of trypsin and papain, and the composition ratio of trypsin to papain is 3:2; (3) After the enzymolysis is completed, the enzymolysis liquid is subjected to solid-liquid separation, and the solid matter in the fermentation liquid is removed through a centrifugal process to obtain a fish protein polypeptide liquid; (4) The fish protein polypeptide liquid is concentrated to a solid matter content of more than 20% to obtain a fish protein peptide liquid; (5) The concentrated fish protein peptide liquid is subjected to spray drying to prepare a fish protein peptide powder preparation.

[0025] The fish protein peptides prepared according to the process of the patent 2024117998536 are controlled to have the same solid matter content as the examples.

[0026] Determined according to the conventional acid hydrolysis method stipulated in the GB / T18246-2019 standard;

[0027] Amino acid content

[0028] Table 1 Amino acid and polysaccharide content of fish protein peptides of different examples Group determination index Salt content of amino acid (%) Polysaccharide content (%) Example 1 87.44 2.69 Example 2 61.57 0.43 Example 3 14.68 1.18 Example 4 58.96 1.54 Example 5 91.67 2.52 Comparative Example 1 22.18 0.03 Comparative Example 2 83.05 0.64 The above experimental results show that the content of amino acids in the fish protein peptide of Example 5 is the highest, reaching 91.67%, which is significantly higher than the current reported level; comparing the amino acid content of fish protein peptides of Examples 1-3, it can be found that the amino acid content of Example 1 is significantly higher than the sum of the amino acid contents of Examples 2 and 3, indicating that the microbial fermentation strains SM9913 and Bacillus DFH-1 have a significant synergistic effect in the present application. The amino acid content in the fish protein peptide of Example 3 is only 14.68%, indicating that the fermentation of the microbial strain Bacillus DFH-1 does not have a significant technical effect on improving the amino acid content, which shows that the microbial strain Bacillus DFH-1 does not produce a large amount of protein-degrading enzymes during the fermentation process. At the same time, the experimental results of Example 4 show that the addition of too much microbial strain Bacillus DFH-1 may inhibit the fermentation enzyme production of marine Pseudoalteromonas SM9913. At the same time, the polysaccharide content results show that a large amount of microbial polysaccharide is produced during the complex microbial fermentation process, which has a significant improvement effect compared to the process of fermenting enzymes first and then enzymolysis.

[0029] 1. Preparation of fish protein peptide (1) After mixing aquatic animal protein with water, grinding and stirring are performed to obtain a mixed slurry, and the pH value is adjusted to be above 7.0, wherein the mixing ratio of aquatic animal protein to water is 1:1.8, and 5% soybean meal, 0.2% Na2HPO47 kg, 0.01% KH2PO4, and 0.03% CaCl2 are added to the slurry; (2) The mixed slurry is added with a complex fermentation strain and stirred uniformly, and the addition amount of the complex fermentation strain is 3% of the mixed slurry, the fermentation temperature is 40-60°C, and the fermentation time is 36 h; the complex fermentation strain is a mixed strain of Pseudoalteromonas SM9913 and Bacillus DFH-1, and the matching ratio is 3:1; (3) After the fermentation is completed, the fermentation liquid is subjected to solid-liquid separation, and the solid matter of the fermentation liquid is removed by centrifugation to obtain a fish protein polypeptide liquid; (4) The fish protein polypeptide liquid is concentrated to a solid content of above 20% to obtain a fish protein peptide.

[0030] 2. Preparation of water-soluble fertilizer (1) Amino acid-containing water-soluble fertilizer: compounded with trace elements, enteromorpha extract, etc. to prepare fish protein amino acid-containing water-soluble fertilizer, fish protein amino acid-containing water-soluble fertilizer, etc., the products have been marketed, and the enterprise standard “Amino acid-containing water-soluble fertilizer” has been formulated, standard number Q / 370202WXS 109-2025; (2) Organic water-soluble fertilizer: fish protein peptide is compounded with Enteromorpha polysaccharide, mineral humic acid, etc. to prepare an organic water-soluble fertilizer "fish protein", which can be foliar sprayed and applied by irrigation and is suitable for crops such as tomatoes and grapes, and has the functions of fruit bulking (fruit bulking rate is more than 15.8%), water and fertilizer retention (improving drought resistance), and phosphorus and calcium activation (activating soil calcium and phosphorus). Fish protein peptide is compounded with seaweed polysaccharide complex to prepare a GPA functional factor complex, and a water-soluble fertilizer "fish protein double power" is prepared, which can be applied by aerial spraying or drip irrigation and is suitable for economic crops and field crops.

[0031] (3) Humic acid-containing water-soluble fertilizer: fish protein peptide is compounded with humic acid to prepare a humic acid-containing water-soluble fertilizer "fish protein potassium", which can enhance the stress resistance of crops, promote fruit color change and sweetness, and is suitable for grape and tomato fruiting periods.

[0032] 1. Preparation of fish protein peptide (1) After mixing aquatic animal protein with water, grinding and stirring are performed to obtain a mixed slurry, and the pH value is adjusted to be higher than 7.0, wherein the mixing ratio of aquatic animal protein to water is 1:1.8, and 5% soybean meal, 0.2% Na2HPO4 7 kg, 0.01% KH2PO4 and 0.03% CaCl2 are added to the slurry; (2) Compound fermentation bacteria are added to the mixed slurry and stirred uniformly, the addition amount of the compound fermentation bacteria is 3% of the mixed slurry, the fermentation temperature is 40-60°C, and the fermentation time is 36 h; the compound fermentation bacteria are a mixed bacteria of Pseudoalteromonas sp. SM9913 and Bacillus DFH-1, and the matching ratio is 3:1; (3) After fermentation, the fermentation liquid is subjected to solid-liquid separation, and the solid content of the fermentation liquid is removed by centrifugation to obtain a fish protein polypeptide liquid; (4) The fish protein polypeptide liquid is concentrated to a solid content of more than 20% to obtain fish protein peptide.

[0033] 2. Preparation of microbial fertilizer Fish protein peptide is compounded with microbial agents, seaweed polysaccharide, polyglutamic acid, trace elements, etc. according to a certain proportion to prepare a microbial fertilizer "fish protein valley" product, which is suitable for various crops including strawberries, peppers, grapes, vegetables, etc. and can be applied by drip irrigation, irrigation or foliar spraying, etc. The product can significantly enhance the resistance of crops to adversity such as low temperature, drought and salinization, and improve the utilization rate of nitrogen, phosphorus and potassium by adjusting the soil nutrient release and plant absorption efficiency.

[0034] Example 9 Application experiment of fish protein peptide in fertilizer 1. Experimental product Experimental group: fish protein valley product of the company, containing effective viable bacteria ≥1 billion / g, fish protein peptide ≥50000 ppm according to the present application Control group: microbial inoculant, containing effective viable bacteria number ≥1 billion / g, not containing fish protein peptide, and the rest of the components are the same as the experimental group.

[0035] 2. Test crops and periods Cucumber seedling stage (transplanted for 5 days) 3. Test time and place Test time: August 20, 2024-September 8; Fertilization time: August 20 and August 29; Fertilization method: 1 bucket / acre of flushing Place: Hedian Town, Hedian Village, Xian County, Liaocheng City 4. Test design and method There are 2 mu of cucumber greenhouse, and the control (1 bucket / acre) and the treatment (1 bucket / acre) are each flushed for 1 mu, and each is flushed for 1 time on August 20 and August 29, and the effect is tracked on August 29 and September 8.

[0036] Investigation index: root number (adventitious root + first-order lateral root), longest root length, total root number (adventitious root + first-order lateral root) on September 8 5. Test results and analysis (1) Tracking the influence of Wenxi Yugu flushing on cucumber roots on August 29 (the effect of the first flushing) From Figure 1 It can be seen that after the first application of fish Yugu (1 bucket / acre), the root system is observed 9 days later, and compared with the control (Hanhe microbial inoculant), the root system number and root health degree (the root system of fish Yugu flushing is white, and the root system of the control is yellowish) of the cucumber root system of fish Yugu flushing are significantly superior, indicating that fish Yugu has better root growth and root maintenance effects.

[0037] (2) Tracking the influence of Wenxi Yugu flushing on cucumber roots on September 8 (the effect of the second flushing) From Figure 2 , 3 , 4 can be seen that after the second application of fish Yugu (1 bucket / acre), the root system is observed 10 days later, and compared with the control (Hanhe microbial inoculant), the cucumber root system of fish Yugu flushing is excellent in root system number (adventitious root + first-order lateral root), longest root length and total root length (adventitious root + first-order lateral root), and the growth rates are 72.4%, 21.4% and 93.3% respectively, indicating that fish Yugu has better root growth effect.

[0038] The above experimental results show that after the fish protein peptide of the present application is used in biological fertilizer, compared with the microbial inoculant with the same microbial content, the fish protein peptide has outstanding technical effects in root growth and root maintenance.

[0039] The above examples are only used for illustrating the technical solutions of the present application, but not for limiting the same; although the present application has been described in detail with reference to the foregoing examples, the technical solutions recorded in the foregoing examples can still be modified, or some of the technical features can be replaced by the equivalent ones, by the ordinary skilled in the art; and the modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions claimed by the present application.

Claims

1. A process for preparing highly absorbable fish protein peptides, characterized in that... Specifically, the steps include the following: (1) Mix aquatic animal protein with water and then grind and stir to obtain a mixed slurry, and adjust the pH value to above 7.0; (2) Add compound fermentation bacteria to the mixed slurry and stir evenly. The amount of compound fermentation bacteria added is 3% to 5% of the mixed slurry. The fermentation temperature is 40 to 60℃ and the fermentation time is 24 to 36 hours. (3) After fermentation, the fermentation liquid is separated into solid and liquid components. The solids in the fermentation liquid are removed by filtration or centrifugation to obtain fish protein polypeptide liquid. (4) Concentrate the fish protein polypeptide solution until the solid content is more than 20% to obtain fish protein peptide.

2. The preparation process of a highly absorbable fish protein peptide according to claim 1, characterized in that... The mixing ratio of the aquatic animal protein to water is 1:1.5 to 2.

0.

3. The preparation process of a highly efficient absorbed fish protein peptide according to claim 1, characterized in that... The mixed slurry contains 5% soybean meal, 0.2% Na2HPO4 (7 kg), 0.01% KH2PO4, and 0.03% CaCl2.

4. The preparation process of a highly efficient absorbed fish protein peptide according to claim 1, characterized in that... The compound fermentation strain is one or a mixture of two of the following: Pseudomonas alterniflora SM9913 and Bacillus DFH-1.

5. The preparation process of a highly absorbable fish protein peptide according to claim 4, characterized in that... The compound fermentation strain is a mixture of Pseudomonas alterniflora SM9913 and Bacillus DFH-1, with a composition ratio of 3:

1.

6. The preparation process of a highly absorbable fish protein peptide according to claim 1, characterized in that... The mixed slurry contains 0.1-0.5% of a complex protease, which is one or a mixture of trypsin, pepsin, and papain.

7. The preparation process of a highly absorbable fish protein peptide according to claim 4, characterized in that... The activation culture medium for Pseudomonas alterniflora SM9913 and Bacillus DFH-1 was prepared with 1% peptone, 0.5% yeast extract, and natural seawater, pH 8.0, and autoclaved at 121ºC for 20 min.

8. A highly absorbable fish protein peptide according to claim 1.

9. The application of the fish protein peptide according to claim 8 in fertilizers such as bio-organic fertilizer, organic-inorganic compound fertilizer, foliar fertilizer, fertigation fertilizer, and microbial fertilizer.

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