Ecological-imitating young salamander breeding method

Abstract

The invention relates to the technical field of aquaculture, in particular to a bionic young salamander breeding method, which remarkably improves the stress resistance of young salamanders on the premise of guaranteeing the survival rate through the synergistic effect of a dynamic environment simulation system, sectional bionic feed and staged environment challenge training. The dynamic environment simulation system regulates and controls multi-parameter dynamic change based on a fuzzy PID algorithm; the bionic feed adopts a segmented nutrition design and is combined with an intestinal targeted embedding technology; according to the environment challenge training, gradient gradual temperature fluctuation (+ / -0.5 DEG C to + / -1.5 DEG C), water flow disturbance (0.05 m / s to 0.15 m / s) and water quality fluctuation (turbidity is 5-15 NTU, pH is 6.8-7.2 oscillation) are matched with a nutrient enrichment and intelligent early warning system, and the stress resistance is directionally improved. A solution with ecological property and stress resistance is provided for large-scale breeding of the young salamanders.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of aquaculture technology, in particular to a bionic ecological juvenile giant salamander breeding method. BACKGROUND

[0002] In the artificial breeding of giant salamanders, the cultivation of juvenile salamanders with stress resistance is a key bottleneck restricting the development of the industry. The existing technology has the following limitations: 1. Lack of stress resistance training: Traditional breeding pursues environmental stability and ignores the driving effect of periodic fluctuations in natural habitats on the adaptability evolution of juvenile salamanders. Studies have shown that in natural conditions, juvenile salamanders need to experience 10-15℃ water temperature fluctuations, 0.08-0.2m / s water flow changes, and sudden increases in turbidity during the rainy season, etc. Environmental challenges, while constant environments in artificial conditions lead to the degradation of their stress regulation mechanisms, and the survival rate in natural water is less than 30%.

[0003] 2. Single environmental regulation: Existing bionic technology mainly focuses on simulating static parameters and lacks multi-dimensional coordinated stimulation. Juvenile salamanders are exposed to complex changes in temperature, water flow, and light in natural streams, and single-parameter regulation cannot activate their comprehensive stress resistance pathways, resulting in abnormally high cortisol levels and a growth inhibition rate of 15-20%.

[0004] 3. Lack of stress management technology: When the environment changes suddenly, traditional methods rely on drug sedation (such as using glutaraldehyde), which can temporarily reduce the activity of juvenile salamanders, but will damage their immune system. Experiments at a breeding farm showed that frequent use of sedatives led to a 40% decrease in lysozyme activity in juvenile salamanders, and a 25% increase in secondary infection rate.

[0005] 4. Lack of standard evaluation of stress resistance: The existing technology has not established a systematic evaluation system for stress resistance, and only uses survival rate to judge the breeding effect. In fact, the temperature tolerance time and hypoxia tolerance of juvenile salamanders are better indicators of their adaptability potential, and these parameters have shown a downward trend under traditional breeding models.

[0006] In addition, the existing feed mainly uses fish meal and soybean meal as the main protein source, which lacks the design of the nutritional needs of juvenile salamanders at specific growth stages. The digestive enzyme activity of juvenile salamanders (0-90 days old) is relatively low, and the protein utilization rate of traditional feed is less than 40%, resulting in a feed conversion ratio of more than 3.5. At the same time, the existing feed generally lacks immune-enhancing ingredients, and the infection rate of juvenile salamanders with iridovirus can reach 15-20%.

[0007] Therefore, it is urgent to develop a systematic breeding method that takes into account survival rate and stress resistance. SUMMARY

[0008] In view of the problems in the prior art, the present application provides a kind of bionomics of young newt breeding method, through integration dynamic environment simulation, segmented nutrition regulation and environmental challenge training, realize the synergistic promotion of young newt resistance and growth performance.

[0009] The technical scheme adopted by the present application to solve its technical problems is: a kind of bionomics of young newt breeding method, comprising the following steps: Step (1) Construct dynamic environment simulation system: through multi-parameter sensor real-time monitoring water quality, illumination, temperature parameter, by fuzzy PID controller adjustment equipment (including ground source heat pump and phase change energy storage material (PCM, phase change temperature 22 DEG C) combined device), make water temperature maintain 18-22 DEG C (day and night fluctuation ±2 DEG C, summer 14:00-16:00 with 0.5 DEG C / h rate from 20 DEG C to 22 DEG C, winter with 0.3 DEG C / h rate from 15 DEG C to 13 DEG C), dissolved oxygen 5-8 mg / L, pH 6.8-7.5, simulate seasonal light cycle;The system is formed by bionics rock group and is stacked water flow (stream flow rate 0.1-0.3 m / s), the bottom of breeding pond is paved 15-20 cm goose pebble layer (simulate natural habitat) and 1-2 cm quartz sand, configure reverse-flow biological filter bed (water quality purification efficiency ≥85%); Step (2) Implement phase environmental challenge training: based on the physiological index feedback dynamic adjustment challenge intensity of young newt heart rate (benchmark value 30-40 times / minute), respiratory rate (benchmark value 25-35 times / minute), when any index exceeds benchmark value 50%, reduce intensity, specifically includes: a. Temperature stress training: from ±0.5 DEG C, weekly incremental 0.2 DEG C temperature fluctuation amplitude, finally stabilize in ±1.5 DEG C (heart rate fluctuation ≤20%); Summer 14:00-16:00 every day with 0.5 DEG C / h rate from 20 DEG C to 22 DEG C, winter every week 3 times with 0.3 DEG C / h rate from 15 DEG C to 13 DEG C; b. Water flow disturbance training: initial flow rate 0.05 m / s, increase 0.02 m / s to 0.15 m / s every month, start 1 time every 3 days 0.12 m / s "flood peak mode" for 1 hour;Through underwater camera monitoring young newt distribution, when the density of a certain area is >30 tails / m², automatically adjust the angle of guide plate to change water flow distribution; c. Water quality fluctuation training: every month 2 times adopt food grade kaolin suspension with particle size 5-10 μm, turbidity is improved from 5 NTU to 15 NTU and maintains 4 hours (2 hours to recover to benchmark value); Every two weeks through 0.1 mol / L sodium bicarbonate-citric acid buffer solution, make pH oscillate between 6.8-7.2 (amplitude ≤±0.3); Step (3) segmented type bionics feed feeding: Juvenile newt period (0-90 days old): feed by earthworm protein, yellow mealworm pupa powder, 1 × 107 -5×10 7 Microencapsulated feed consisting of Lactobacillus rhamnosus YFI-6 (sodium alginate-chitosan composite membrane as embedding wall material, embedding rate ≥ 90%, slow-release rate ≤ 10% in 2 hours in the stomach acid environment of juvenile newts); Juvenile newt period (91-180 days old): feeding with 1 g / mL crude drug concentration of Artemisia argyi-Isatis indigotica composite extract (preparation method: Artemisia argyi and Isatis indigotica are mixed at a ratio of 1:1, 8 times the volume of 70% ethanol is added, ultrasonic extraction for 3 times, 30 minutes each time, and the extract is combined and concentrated) and 5×10 6 -1×10 7 Puffed feed containing 1×10 The protein content of the biomimetic feed is 45-55% for juvenile newts and 40-50% for juvenile newts, and the fat content is controlled at 8-12%; Step (4) supporting guarantee: Nutritional regulation: feeding with stress feed containing 1% glutamine, 0.5% gamma-aminobutyric acid, and 0.3% nucleotides for 3 days before the challenge, and feeding with recovery feed containing 5% fish oil (rich in EPA / DHA), 0.2% vitamin C, and 2% spirulina powder for 5 days after the end of the challenge; Intelligent monitoring and emergency: deploying a five-dimensional monitoring network combining machine vision and LSTM neural network (disease occurrence probability prediction accuracy ≥ 92%), starting emergency response when the parameter exceeds the safety threshold of 80%, including releasing a flexible isolation net and restoring the environmental parameters to 90% of the baseline value within 3 minutes; The juvenile newt breeding density is 30-50 tails / m² for juvenile newts and 15-25 tails / m² for juvenile newts, and independent regulation is adopted in different zones.

[0010] Specifically, in the temperature stress training, 50 mg / kg L-cysteine and 200 mg / kg vitamin E are added to the feed to induce the expression of heat shock protein HSP70 (HSP70 gene relative expression of high gradient group is 2.8 times that of constant temperature control group), so that the survival rate of juvenile newts in 24℃ high temperature tolerance test is ≥ 92%, and the time of first abnormal behavior is ≥ 62 minutes.

[0011] Specifically, the flow disturbance training is divided into zones by flow rate gradient and dynamically allocated, so that the swimming speed of juvenile newts is increased from 0.08 m / s to 0.15 m / s after 90 days of training, the proportion of fast muscle fibers is increased from 45% to 62%, the muscle glycogen content is increased by 38%, and the proportion of activity in the turbulent zone is increased from 5% to 40%.

[0012] Specifically, in the water quality fluctuation training, the pH fluctuation amplitude is controlled to be less than or equal to ±0.3 by using sodium bicarbonate-citric acid buffer solution, so that the acid-base balance regulation enzyme activity of the young newts is increased by 35%; the turbidity is adjusted by using kaolin suspension, so that the olfactory predation success rate of the young newts is increased by 60%, the serum lysozyme activity is increased from 22 U / mL to greater than or equal to 58 U / mL, and the complement C3 content is greater than or equal to 12.5 mg / dL.

[0013] Specifically, the emergency response includes releasing a flexible isolation net, and restoring the environmental parameters to 90% of the reference value within 3 minutes; when simulating the summer rainstorm environmental mutation (water temperature rises by 3°C, dissolved oxygen decreases to 4.5 mg / L, and turbidity increases to 18 NTU) scenario, the mortality rate of the young newts is less than or equal to 5%, and the duration of abnormal behavior is less than or equal to 1.5 hours.

[0014] Specifically, the bionic rock group of the dynamic environment simulation system forms a laminated water flow, and the stream flow rate is controlled to be 0.1-0.3 m / s; in combination with the ground source heat pump and the PCM combined device, the energy saving is 40% compared with the traditional heating rod, and the fitting degree R² of the water temperature fluctuation curve to the natural stream is greater than or equal to 0.92.

[0015] Specifically, the microcapsule embedding wall material of the bionic feed is a sodium alginate-chitosan composite film, the embedding rate is greater than or equal to 90%, the release rate is less than or equal to 10% in the stomach acid environment of the young newts within 2 hours, so that the feed protein utilization rate of the juvenile newts is greater than or equal to 62%; the inhibition rate of the artemisia-coughs and sneezes complex extract on the iridovirus is greater than or equal to 78%, in combination with bacillus subtilis, the intestinal IgM content of the young newts is increased by 40%, and the iridovirus infection rate is less than or equal to 5%; The preparation method of the artemisia-coughs and sneezes complex extract is: mixing artemisia and coughs and sneezes at a ratio of 1:1, adding 8 times the volume of 70% ethanol, ultrasonic extraction at 50°C for 3 times, each time for 30 minutes, combining the extract and concentrating to a crude drug concentration of 1 g / mL; The protein content of the bionic feed is: 45-55% for the juvenile newts, and 40-50% for the young newts, and the fat content is controlled to be 8-12%.

[0016] Specifically, the breeding density of the young newts is: 30-50 tails / m² for the juvenile newts, and 15-25 tails / m² for the young newts, and the independent regulation is adopted in different zones; in combination with the segmented bionic feed feeding, the average daily weight gain of the young newts is greater than or equal to 1.1 g / d, and the feed conversion ratio is reduced from 3.2 to less than or equal to 2.1.

[0017] Specifically, the five-dimensional monitoring network combines machine vision and LSTM neural network, and by monitoring the behavior parameters such as swimming frequency and feeding frequency of the young newts, the prediction accuracy of disease occurrence probability is greater than or equal to 92%, which is 15 minutes earlier than artificial observation to find stress abnormalities.

[0018] Specifically, the bottom of the culture pond is paved with 15-20 cm of pebbles and 1-2 cm of quartz sand, and a countercurrent biological filter bed is configured to purify water quality (water quality purification efficiency is greater than or equal to 85%), so that the dissolved oxygen content of the culture water body is stably maintained at 5-8 mg / L, and the turbidity is long-term less than or equal to 15 NTU; the juvenile salamanders cultured for 180 days have the floating head time prolonged from 18 minutes to greater than or equal to 28 minutes in a 4 mg / L low-oxygen environment, and the survival rate after being released into a natural stream is greater than or equal to 1 times that of traditional culture.

[0019] The beneficial effects of the present application are: 1. The resistance is significantly improved: the juvenile salamanders trained for 180 days have the first abnormal behavior appearing time prolonged from 35 minutes to 62 minutes in a 24℃ high temperature tolerance test, and the floating head time prolonged from 18 minutes to 28 minutes in a 4 mg / L low-oxygen environment, which is improved compared with traditional culture.

[0020] 2. The growth performance is synergistically optimized: although the environmental challenge is increased, the daily average weight gain of the juvenile salamanders is still 1.15 g / d (0.8 g / d by traditional method), and the feed conversion ratio is reduced from 3.2 to 2.1, realizing the synchronous improvement of resistance and growth speed.

[0021] 3. The ecological adaptability is enhanced: the release experiment shows that the survival rate of the juvenile salamanders cultured by the present method in the natural stream is improved, which provides high-quality fry for propagation and release. BRIEF DESCRIPTION OF DRAWINGS

[0022] The present application will be further described below in conjunction with the drawings and examples.

[0023] Figure 1 A flow chart of a bionic ecological juvenile salamander culture method provided by the present application. DETAILED DESCRIPTION

[0024] In order to make the technical means, creative features, purposes and effects realized by the present application easy to understand, the present application will be further described below in conjunction with specific embodiments.

[0025] As shown in the drawings, Figure 1 A bionic ecological juvenile salamander culture method according to the present application comprises the following steps: 1. Stage environmental challenge training Gradient progressive mechanism: adopt the mode of "physiological index feedback-challenge intensity dynamic adjustment". The heart rate (baseline value 30-40 times / minute) and respiratory rate (baseline value 25-35 times / minute) of the juvenile salamander are monitored by implantable sensors, and when any index exceeds 50% of the baseline value, the challenge intensity is automatically reduced. For example, the temperature fluctuation amplitude starts from ±0.5℃, and increases by 0.2℃ every 7 days, until the juvenile salamander can stably adapt to ±1.5℃ fluctuation (heart rate fluctuation is less than or equal to 20%).

[0026] Multi-dimensional coordinated stimulation: design "physical-chemical-biological" compound challenge scenarios. In the "rainstorm simulation" once a week, the following are achieved simultaneously: ① Pulse water jet (intensity 5 mm / h, duration 30 min); ② Water flow rate from 0.08 m / s to 0.1 m / s; ③ Water surface fluctuation (wave height 3-5 cm); ④ Dissolved oxygen content drops to 5 mg / L.

[0027] This compound stimulation can activate the coordinated regulation ability of the hypothalamic-pituitary-adrenal axis in juvenile salamanders, and the anti-stress gene expression is increased by 2.3 times compared with single stimulation.

[0028] Intelligent safety management and control: build a five-dimensional parameter safety matrix (Table 1). When the monitoring value reaches 80% of the safety threshold, start the hierarchical response.

[0029] First-level response (e.g., dissolved oxygen 4.8 mg / L) only starts oxygenation; Second-level response (e.g., dissolved oxygen 4.5 mg / L) releases 0.3 mg / L of rhubarb extract at the same time; Third-level response (e.g., dissolved oxygen 4.2 mg / L) immediately opens physical isolation and transfers affected juvenile salamanders to the emergency protection area.

[0030] Table 1 Environmental parameter safety threshold matrix:

[0031] 2. Key challenge training implementation Temperature stress training system: use "ground source heat pump + phase change energy storage material (PCM)" combination device. In summer, PCM (phase change temperature 22℃) absorbs heat from 14:00 to 16:00, slowly raises the water temperature to 22℃ (temperature rise rate 0.5℃ / h), and then cools down naturally through the ground source heat pump; In winter, it is operated in reverse to achieve controllable cooling from 15℃ to 13℃. This system is 40% more energy efficient than traditional heating rods, and the temperature fluctuation curve is closer to natural stream (R²=0.92).

[0032] Water flow disturbance training device: composed of variable frequency water pump, adjustable guide vane and flow rate partition controller. In a 50m² breeding pond, three flow rate zones are divided: ① static water zone (0.05-0.08 m / s); ② transition zone (0.08-0.12 m / s); ③ turbulent flow zone (0.12-0.15 m / s). Through real-time monitoring by camera, when the habitat density in a certain area exceeds 30 individuals / m², the guide vane angle is automatically adjusted to change the water flow distribution, forcing the juvenile salamanders to migrate to low-density areas, and improving their adaptation range to flow rate changes.

[0033] Water quality fluctuation training: Turbidity regulation: Food-grade kaolin (particle size 5-10 μm) was used to configure the suspension, and the turbidity was increased from 5 NTU to 15 NTU (for 4 hours) by injecting water into the water body twice a month through a metering pump, and then the sand filter-activated carbon combined system was started, and the turbidity was restored to the baseline value within 2 hours. This training can reduce the dependence of juvenile salamander visual feeding and improve the success rate of olfactory predation by 60%.

[0034] pH shock: Through the double-pump linkage system, 0.1 mol / L sodium bicarbonate and citric acid solutions were alternately injected, and the pH was fluctuated in the range of 6.8-7.2 once every 12 hours (amplitude ±0.2), which increased the acid-base balance enzyme activity of juvenile salamander in the static pH environment by 35%.

[0035] 3. Nutrient fortification Precise nutrition during stress period: Special stress feed was fed 3 days before the challenge, containing 1% glutamine (intestinal mucosa protection), 0.5% gamma-aminobutyric acid (central nervous system sedation) and 0.3% nucleotide (immune activation). Comparative experiments show that this formula can reduce the cortisol peak of juvenile salamander from 85 ng / mL to 59 ng / mL, and the feeding inhibition rate from 40% to 18%.

[0036] Nutritional repair during recovery period: 5 days after the challenge, high repair bait was fed: ① 5% fish oil (rich in EPA / DHA, promote cell membrane repair); ② 0.2% vitamin C (antioxidant); ③ 2% spirulina powder (provide natural pigment and minerals). This stage can increase the glutathione peroxidase activity of juvenile salamander liver by 45%, and accelerate the muscle damage repair speed by 30%.

[0037] Segmented bionic feed formula Juvenile salamander period (0-90 days old): Using microcapsule embedding technology, earthworm protein, yellow mealworm pupa powder and lactobacillus rhamnosus YFI-6 (3×10 7 CFU / g) were wrapped in sodium alginate-chitosan composite film. This design can make the feed slowly release in the stomach of juvenile salamander, and the protein utilization rate is increased to 62%, and the probiotic colonization rate is increased by 40%.

[0038] Juvenile salamander period (91-180 days old): Artemisia-Isatis root composite extract and bacillus subtilis (8×10 6 CFU / g) were added, and the floating granular feed was made by puffing process. In vitro experiments show that the inhibition rate of the feed on iridovirus can reach 78%, and the fat deposition rate is reduced by 15%.

[0039] Example one: Effect of temperature gradient training on heat shock protein expression Experimental design: Set up 3 treatment groups, 3 replicates for each group (50 tails per replicate, initial weight 5.2±0.3g): Control group: constant temperature 20℃ Low gradient group: temperature fluctuation gradually increased from ± 0.5℃ to ± 1.0℃ (0.2℃ per week) High gradient group: temperature fluctuation gradually increased from ± 0.5℃ to ± 1.5℃ (0.2℃ per week) Training period: 90 days, daily monitoring of juvenile salamander heart rate and food intake.

[0040] Feed intervention: All treatment groups added 50mg / kg L-cysteine and 200mg / kg vitamin E during temperature fluctuation period.

[0041] Test results: The relative expression of HSP70 gene in the high gradient group was 2.8 times that of the control group, and 1.9 times in the low gradient group; The survival rate of the high gradient group in the 24℃ tolerance test was 92%, significantly higher than that of the control group (68%); The average daily weight gain of the high gradient group was 1.02g / d, which had no significant difference (P>0.05) with the control group (1.05g / d), proving that gradient training did not affect growth.

[0042] Technical effect of dynamic environment simulation system: In this embodiment, the dynamic environment simulation system composed of "ground source heat pump + phase change energy storage material (PCM)", combined with the parameter adjustment logic of fuzzy PID controller, realizes three core effects: first, precise temperature control and natural simulation are highly consistent. Through the cooperation of PCM (phase change temperature 22℃) and ground source heat pump, the water temperature fluctuation curve is fitted with natural stream with R²=0.92, avoiding the disadvantages of traditional heating rod "sudden rise and fall"; second, energy saving and stability are considered, which is 40% more energy saving than traditional temperature control equipment, and can long-term maintain the water temperature in the reference interval of 18-22℃, with day and night fluctuation accurately controlled within ±2℃, meeting the gradient regulation and control requirements of temperature stress training; third, dynamic response is timely, which can be heated at a preset rate of 0.5℃ / h from 14:00 to 16:00 in summer, and cooled at a rate of 0.3℃ / h in winter, with a response delay of ≤5 minutes, providing a stable environmental basis for temperature gradient training.

[0043] The effect of the phased environmental challenge training technology: the temperature gradient training mode of "physiological index feedback-intensity dynamic adjustment" achieved significant anti-reversal activation effect: first, the anti-reversal pathway was activated, and the fluctuation training gradually increased from ± 0.5℃ to ± 1.5℃, which accurately triggered the coordinated regulation of the hypothalamic-pituitary-adrenal axis in young salamanders. The relative expression of HSP70 gene in the high gradient group was 2.8 times that of the control group, and the efficient expression of heat shock protein directly improved the high temperature tolerance; second, the tolerance threshold was significantly improved, and in the 24℃ high temperature tolerance test, the survival rate of the high gradient group was 92% (the control group was only 68%), and the time of the first abnormal behavior was extended from 35 minutes to 62 minutes, which proved that the gradient progressive training could effectively expand the temperature tolerance range; third, the stress adaptability was enhanced, and through real-time monitoring of heart rate (baseline 30-40 times / minute) and respiratory rate (baseline 25-35 times / minute), excessive stress was avoided, so that the physiological state of young salamanders during training was always in the "controllable challenge" interval, and there was no large-scale stress death.

[0044] The effect of the segmented bionic feed and environmental challenge deep synergy technology: the synergy mode of "temperature stress training + special feed addition (50mg / kg L-cysteine + 200mg / kg vitamin E)" in this embodiment achieved the dual goals of "anti-reversal improvement-growth guarantee": first, the feed assisted the expression of anti-reversal genes, L-cysteine as an antioxidant could reduce the active oxygen damage caused by temperature stress, and vitamin E could promote the stability of cell membrane, and the two together activated the expression of HSP70, making the HSP70 expression of the high gradient group increased by 15% compared with the "training only without feed addition" group; second, the nutritional supply matched the training needs, and the protein content of the segmented feed (45-55% for young salamanders) accurately met the energy consumption of young salamanders under temperature stress, avoiding the growth stagnation caused by training-the average daily weight gain of the high gradient group was 1.02g / d, which had no significant difference (P>0.05) with the constant temperature control group (1.05g / d), breaking the traditional limitation that environmental challenge must sacrifice growth; third, the stress damage was prevented in advance, and the functional ingredients in the feed could enhance the stress tolerance basis of young salamanders in advance, making the cortisol peak value when the temperature fluctuated 20% lower than that of the "no feed assistance" group, reducing the inhibition of stress on growth hormone secretion.

[0045] Synergistic effect exceeds expected technical effect: The combination of precise temperature control of dynamic environment simulation system, gradient activation of phased temperature challenge training, and nutritional synergy of segmented feed produces an unexpected effect: first, the resistance performance is improved in a leap, the high gradient group not only improves the high temperature survival rate (92%) by 35% compared with the control group (68%), but also the HSP70 gene expression is 2.8 times that of the control group, far exceeding the single effect of "system temperature control only" or "feed addition only" (single measure HSP70 improvement does not exceed 1 times); second, growth and resistance are not in conflict, in traditional breeding, if forced to conduct temperature challenge, the daily average weight gain of young salamanders will decrease to below 0.6g / d, while under the synergistic scheme, the daily average weight gain of the high gradient group still maintains 1.02g / d, realizing the breakthrough of "significantly improving resistance and not reducing growth"; third, the environmental adaptability is closer to nature, after synergistic training, the young salamanders adapt to temperature fluctuations in a mode closer to wild individuals (heart rate fluctuation ≤20%), laying a foundation for subsequent propagation and release, while the temperature adaptation period of traditional constant temperature breeding young salamanders needs to be extended by 2 times.

[0046] Example two: Effect of compound water flow training on the movement ability of young salamanders Device parameters: 100m² breeding pond is divided into three zones, initial flow rate: 0.05m / s in static water zone, 0.08m / s in transition zone, and 0.12m / s in turbulent flow zone, increasing the flow rate of each zone by 0.02m / s every month.

[0047] Behavior monitoring: The intelligent trajectory analysis system (sampling frequency 10 frames / second) is used to record the activity time proportion of young salamanders in each zone.

[0048] Training effect: On the 30th day: young salamanders spend 75% of their time in the static water zone and only 5% in the turbulent flow zone; On the 90th day: the proportion in the static water zone decreases to 30% and the proportion in the turbulent flow zone increases to 40%, and the swimming speed increases from the initial 0.08m / s to 0.15m / s; Muscle component analysis shows that the proportion of fast muscle fibers increases from 45% to 62%, muscle glycogen content increases by 38%, and exercise endurance is significantly enhanced.

[0049] Technical effects of the dynamic environment simulation system: The "flow rate gradient partition + intelligent regulation" dynamic environment simulation system constructed in this embodiment realizes the accurate reproduction of natural stream flow rate scenarios: first, multiple flow rate scenarios are simulated simultaneously by using variable frequency water pumps and adjustable guide plates to divide "still water area (0.05-0.08 m / s) - transition area (0.08-0.12 m / s) - turbulent flow area (0.12-0.15 m / s)", accurately reproducing the flow rate heterogeneity of the wild juvenile newt habitat, which is closer to nature than the traditional "single flow rate" breeding pool (natural stream flow rate fluctuation range 0.08-0.2 m / s); second, dynamic self-adaptive regulation, combined with underwater cameras and fuzzy PID algorithm, real-time monitoring of juvenile newt habitat density (when a certain area > 30 tails / m², automatically adjusting the guide plate), so that the flow rate partition ratio is dynamically optimized with the adaptive capacity of juvenile newts, avoiding the adaptive bottleneck caused by fixed flow rate; third, high flow rate regulation accuracy, flow rate regulation error ≤0.01 m / s, controllability strong at a rate of 0.02 m / s per month, ensuring that the water flow challenges are "gradient progressive" and there is no juvenile newt stress caused by flow rate sudden change (such as collision, feeding stagnation).

[0050] The technical effects of the phase environment challenge training: The "flow rate gradually increasing + partition migration guiding" phase training significantly improves the movement ability and environmental adaptability of juvenile newts: first, the movement ability is enhanced, after 90 days of training, the juvenile newt swimming speed increases from the initial 0.08 m / s to 0.15 m / s, the fast muscle fiber proportion increases from 45% to 62% (fast muscle fiber determines the explosive power and endurance), the muscle glycogen content increases by 38% (energy reserve is improved), and the exercise endurance is 1.8 times higher than before training; second, the flow rate adaptation range is greatly widened, the adaptability of juvenile newts to turbulent flow area increases from "5% of activity on the 30th day" to "40% on the 90th day", breaking the traditional breeding habit of "preferring still water area and fearing turbulent flow" of juvenile newts, and being closer to the flow rate adaptation characteristics of wild individuals; third, the behavior regulation mechanism is activated, during the training process, juvenile newts adjust their swimming posture (such as body bending degree and tail fin swinging frequency) to adapt to different flow rates, triggering the motor nerve regulation pathway, and the movement coordination significantly improves (trajectory analysis shows that the stability of the swimming trajectory is improved by 50%).

[0051] Technical effect of segmented bionic feed and environmental challenge deep coordination: The nutritional design of segmented bionic feed provides "energy-structure" double support for water flow training, realizing the coordination of "training promoting movement-feed preserving growth": First, the energy supply matches the training consumption. The protein content of the feed for young salamanders is 40-50%, and the fat content is 8-12%, which accurately meets the energy needs of muscle activity in water flow training (energy consumption is 30% higher than static cultivation), avoiding "energy negative balance" caused by training. The muscle protein deposition rate of the training group increased by 22% compared with the "training only without special feed" group. Second, the nutritional ingredients promote muscle development. The earthworm protein and mealworm pupa powder in the feed are rich in essential amino acids (such as leucine and isoleucine), providing raw materials for fast muscle fiber proliferation, and the proportion of fast muscle fiber increases by 15% compared with the "no feed assistance" group. Third, probiotics assist in movement recovery. The Bacillus subtilis (5x10 6 -1x10 7 CFU / g) in the young salamander feed can improve the efficiency of intestinal digestion and absorption, making the feed protein utilization rate reach 58% (only 40% for traditional feed), ensuring that young salamanders can quickly replenish nutrients after training and reduce the accumulation of exercise fatigue.

[0052] Synergistic effect beyond expectations: The three-party coordination of system, training, and feed produces a movement capacity improvement effect far beyond a single measure: First, the adaptive behavior is completely changed. After 90 days of traditional water flow training (without gradient, without feed assistance), the young salamanders' activity in the turbulent flow area accounts for less than 10%, while the proportion under this coordination scheme reaches 40%, and the swimming posture is stable without obvious stress behaviors (such as rapid tail swinging and hiding). Second, muscle function and energy reserve are optimized. Not only does the proportion of fast muscle fibers increase, but the muscle glycogen content also increases, making the young salamanders' sustained swimming time at a flow rate of 0.15 m / s reach 45 minutes, which is 1.25 times that of the "system control flow only" group (20 minutes). Third, growth and movement capacity are simultaneously improved. The daily average weight gain of young salamanders in the coordination group is 1.1 g / d, which is 47% higher than that of the "training only without special feed" group (0.75 g / d), achieving the unexpected effect of "enhanced movement capacity and accelerated growth", breaking the traditional cognition that "high-intensity training must lead to reduced growth".

[0053] Example Three: Effect of water quality fluctuation training on immune indicators Fluctuation scheme: Conduct 1 complex water quality challenge every two weeks: pH 6.8→7.2→6.8 (12-hour cycle) + turbidity 5→15→5 NTU (4-hour cycle), and monitor the immune indicators of young salamanders simultaneously.

[0054] Detection data: Serum lysozyme activity increased from 22 U / mL to 58 U / mL (the control group remained at 25-28 U / mL); Complement C3 content reached 12.5 mg / dL, 2.1 times of the control group; After artificial infection with Aeromonas hydrophila, the mortality rate of the training group was 12%, which was significantly lower than that of the control group (45%).

[0055] The technical effect of the dynamic environment simulation system: The "water quality parameter precise regulation + rapid recovery" dynamic environment simulation system of the embodiment realizes the safe reproduction of natural water quality fluctuation: first, multi-parameter collaborative control and stability, through the double-pump linkage system (sodium bicarbonate-lime acid buffer) to control the pH shock amplitude ≤±0.3, using food-grade kaolin suspension (particle size 5-10 μm) to regulate turbidity, ensuring that the pH is accurately controllable in the fluctuation range of 6.8-7.2, and the turbidity is accurately controllable in the fluctuation range of 5-15 NTU, avoiding the poisoning of juvenile newts caused by parameter overrun (such as pH <6.5 will inhibit gill respiration); second, simulate nature and rapid recovery, after the turbidity rises from 5 NTU to 15 NTU, the sand filter-activated carbon combined system recovers to the reference value within 2 hours, and the pH returns to stability within 12 hours after shock, accurately simulating the scene of "turbidity sudden rise-gradual fall" and "pH small amplitude fluctuation" in natural rainy season, and not damaging the overall water quality of the breeding pond (dissolved oxygen always maintains 5-8 mg / L); third, low interference regulation, during the water quality fluctuation process, the system adjusts the parameters (such as kaolin suspension injection rate 0.5 L / min during turbidity adjustment) through "trace multiple injection" method, avoiding the stress of juvenile newts caused by sudden change of parameters (such as sudden rise of turbidity will cause juvenile newts to stop feeding temporarily).

[0056] The technical effect of the phase environment challenge training: The "pH-turbidity compound fluctuation + periodic reinforcement" phase training significantly activates the immune system and environmental adaptation mechanism of juvenile newts: first, the immune function is greatly enhanced, after 90 days of training, the serum lysozyme activity of juvenile newts increases from 22 U / mL to 58 U / mL (the control group maintains 25-28 U / mL), the complement C3 content reaches 12.5 mg / dL (the control group is 6 mg / dL), and the immune-related enzyme activity increases by 2-2.6 times, proving that the training effectively activates the innate immune system; second, the resistance to pathogens is improved, after artificial infection with Aeromonas hydrophila, the mortality rate of the training group is only 12%, which is 73% lower than that of the control group (45%), and the onset time is delayed by 24 hours, indicating that the training enhances the resistance of juvenile newts to pathogenic bacteria; third, the non-visual foraging ability is optimized, turbidity fluctuation training reduces the dependence of juvenile newts on visual foraging, and the success rate of olfactory foraging is improved by 60% (the success rate of olfactory foraging of the control group is only 35%), which adapts to the survival needs of "relying on olfactory foraging in turbid water period" in natural water.

[0057] Technical effects of the segmented bionic feed and the deep synergy with environmental challenges: During the juvenile stage, the "artemisia-isioliquidambar complex extract + bacillus subtilis" bionic feed, combined with water quality fluctuation training, forms a synergistic effect of "immune activation-immune enhancement". First, the feed functional ingredients assist immune training. The artemisia-isioliquidambar complex extract (concentration of crude drug 1 g / mL) has an inhibition rate of 78% on iridovirus, and forms a "bacteria + virus" double defense with the activated innate immunity by water quality training, so that the iridovirus infection rate of juvenile salamanders is reduced from 15-20% in traditional cultivation to less than 5%. Second, the probiotics enhance the intestinal immune barrier. The bacillus subtilis (5 x 10 6 -1 x 10 7 CFU / g) in the feed can colonize in the intestinal tract of juvenile salamanders, promote the secretion of intestinal mucosal immunoglobulin (IgM content increased by 40%), and reduce the risk of harmful bacteria (such as aeromonas) invading through the intestinal tract, so that the incidence of intestinal inflammation in the training group is reduced by 30% compared with the "no probiotic feed" group. Third, the nutritional supply supports immune metabolism. The feed protein (40-50%) and vitamins (such as vitamin C and E) provide raw materials for the proliferation of immune cells (such as macrophages and lymphocytes), so that the immune indicators are more persistent during the training process. After 15 days of stopping training, the synergistic group maintains a lysozyme activity of more than 50 U / mL, while the "training only without special feed" group is reduced to 35 U / mL.

[0058] The synergistic effect is beyond expectation. The synergy of precise control and stabilization of the system, immune activation of training, and immune enhancement of feed produces an unexpected effect of "immunity performance leapfrogging + ecological adaptability enhancement". First, the immune indicators are improved by a much larger margin than single measures. The lysozyme activity of the "system + training + feed" synergistic group (58 U / mL) is increased by 53% compared with the "water quality training only" group (38 U / mL) and by 81% compared with the "feed addition only" group (32 U / mL), proving that the synergistic effect can amplify the immune activation effect. Second, the immune and ecological adaptation are optimized simultaneously. The juvenile salamanders in the synergistic group not only have strong resistance to pathogens, but also have high efficiency foraging ability in turbid water period. When released into natural turbid streams (turbidity 10-15 NTU), the foraging frequency is 2 times higher than that of traditional cultivated juvenile salamanders, and the survival rate reaches 65% (traditional cultivation is only 30%). Third, the immune stability is enhanced. After the water quality fluctuation stops, the immune indicators of the synergistic group decay slowly, maintaining a high immune state, avoiding the drawbacks of traditional training "stop training and immune decline", and providing protection for long-term stress resistance.

[0059] Example Four: Effect of Nutritional Enhancement on Stress Recovery of Juvenile Salamanders Experimental groups: Training + ordinary feed group: only environmental challenge, feeding basic feed; Training + enhanced feed group: 3 days of stress feed before challenge, 5 days of recovery feed after challenge; Control group: no challenge + basal diet.

[0060] Recovery indicators: The training + reinforcement group recovered 90% of the normal level of food intake 24 hours after the challenge, which was significantly higher than the training + ordinary group (65%); The liver MDA content (oxidative damage indicator) decreased to 1.2 nmol / mgprot 48 hours after the challenge, lower than the training + ordinary group (2.5 nmol / mgprot); The recovery rate of intestinal villus length reached 95%, an increase of 30% compared with the training + ordinary group.

[0061] Technical effect of dynamic environment simulation system: This embodiment relies on the dynamic environment simulation system of "five-dimensional monitoring network (machine vision + LSTM neural network) + emergency regulation" to provide precise monitoring and protection for stress recovery: first, real-time perception of stress state, the system monitors the behavior of newts (such as swimming frequency, feeding frequency) through machine vision, and predicts the stress level (accuracy ≥ 92%) combined with LSTM neural network, which can quickly identify stress signals (such as feeding inhibition, rapid swimming) after environmental challenge, 15 minutes earlier than manual observation to find stress abnormalities; second, precise regulation of recovery environment, when the newt is in a state of stress, the system can fine-tune the environmental parameters (such as stabilizing the water temperature at 20℃, increasing the dissolved oxygen to 7mg / L), to create suitable conditions for stress recovery, and avoid environmental fluctuations exacerbating stress damage; third, precise definition of stress threshold, through LSTM neural network analysis of historical data, to determine the critical indicators of newt stress (such as cortisol > 80ng / mL for severe stress), to provide precise timing for the switch of "stress feed - recovery feed", and avoid early or late feed regulation.

[0062] Technical effect of phase environmental challenge training: The phase cycle training of "environmental challenge - stress recovery" significantly improves the stress tolerance and rapid recovery ability of newts: first, the stress regulation mechanism is activated, through multiple "challenge - recovery" cycles, the regulation efficiency of the hypothalamus-pituitary-adrenal axis of newts is improved, and the peak value of cortisol during stress is reduced from 85ng / mL to 59ng / mL (before training), and the stress response intensity is reduced by 31%; second, the recovery speed is greatly accelerated, under the coordination of feed regulation, the food intake recovers to 90% of the normal level 24 hours after the challenge ("no training group" is only 65%), and the recovery rate of intestinal villus length reaches 95% 48 hours after the challenge ("no training group" is only 65%), which proves that training can shorten the stress recovery period; third, the resistance to stress damage is enhanced, the liver MDA content (oxidative damage indicator) of newts after training decreases to 1.2 nmol / mgprot 48 hours after the challenge ("no training group" is 2.5 nmol / mgprot), and the oxidative stress damage is significantly reduced, which shows that training can enhance the self-repairing ability of stress damage.

[0063] The segmented bionic feed and environmental challenge deep synergy technology effect: the segmented nutrition regulation of "stress feed (3 days before challenge) + recovery feed (5 days after challenge)" and environmental challenge training form a synergy of "pre-emptive protection-post-repair": first, pre-emptive protection before stress, 1% glutamine in stress feed can protect the intestinal mucosa (40% reduction in intestinal villus damage rate compared to "no stress feed" group), 0.5% gamma-aminobutyric acid can calm the central nervous system, reduce excessive reaction (such as rapid swimming leading to energy consumption) during stress, and reduce cortisol peak by 22% during challenge; second, precise repair after stress, 5% fish oil (rich in EPA / DHA) in recovery feed can promote cell membrane repair (35% increase in liver cell membrane integrity), 0.2% vitamin C can scavenge oxygen free radicals (52% reduction in MDA content), and 2% spirulina powder provides minerals (such as selenium and zinc) to accelerate muscle and organ damage repair; third, the nutrition supply matches the recovery needs, the recovery feed has a protein content of 45% and a fat content of 10%, and the amino acid composition is closer to the stress recovery needs (such as a 10% increase in lysine content), which increases the protein synthesis efficiency of young newts by 25% in the recovery stage, accelerating body weight recovery.

[0064] The synergistic effect is beyond expectation: the precise monitoring of the system, the stress adaptation of the training, and the nutrition repair of the feed synergize to produce an unexpected effect of "small stress damage + fast recovery + no growth lag": first, the recovery efficiency far exceeds that of a single measure, the recovery rate of the "system + training + feed" synergy group (90%) is 50% higher than that of the "stress training only" group (60%) and 29% higher than that of the "feed regulation only" group (70%), proving that the synergistic effect can greatly accelerate recovery; second, stress and growth are not in conflict, the daily average weight gain of young newts in the synergy group after experiencing multiple "challenge-recovery" cycles is still 1.1g / d, which is basically the same as that of the no-challenge control group (1.05g / d), while the daily average weight gain of the "no feed-assisted training" group is only 0.7g / d, achieving "stress recovery and growth synchronization"; third, long-term stress tolerance is enhanced, after 10 "challenge-recovery" cycles, the mortality rate of young newts in the synergy group under severe stress (such as dissolved oxygen dropping to 4mg / L) is only 8%, which is 77% lower than that of the "no synergy" group (35%), proving that synergy training can build long-term stress tolerance, not short-term recovery.

[0065] Example Five: Application Verification of Intelligent Early Warning System in Extreme Weather Simulation scenario: simulate the environmental mutation caused by summer rainstorm (water temperature rises by 3℃, dissolved oxygen drops to 4.5mg / L, turbidity rises to 18NTU).

[0066] System response: Secondary warning is triggered within 1 minute, and oxygenation device and rhubarb extract release are started; Within 3 minutes, the water temperature dropped by 1.5℃, and the dissolved oxygen rose to 5.2mg / L; Within 10 minutes, the flexible isolation netting was deployed, forming a 5m² emergency protection zone (water temperature 20℃, dissolved oxygen 7mg / L). Comparison results: The mortality rate of juvenile salamanders in the activated system group was 5%, and the duration of abnormal behavior was 1.5 hours. The mortality rate was 32% in the group that did not use the system, and the duration of abnormal behavior was 6 hours.

[0067] Technical Effects of the Dynamic Environment Simulation System: The dynamic environment simulation system constructed in this embodiment, which combines "extreme environment simulation + graded emergency response," achieves safety protection under extreme weather conditions: First, it accurately reproduces extreme scenarios. The system can simulate complex environmental abrupt changes caused by summer rainstorms (a sudden increase in water temperature of 3°C, a drop in dissolved oxygen to 4.5 mg / L, and an increase in turbidity to 18 NTU). The simulation parameters have a 90% match with natural rainstorm scenarios, providing a realistic scenario for extreme stress testing. Second, it provides rapid and efficient graded responses. The system responds according to the degree to which parameters exceed thresholds (80% warning, 100% emergency response). The system initiates tiered measures: Level 1 response (e.g., dissolved oxygen 4.8 mg / L) activates oxygenation; Level 2 response (e.g., dissolved oxygen 4.5 mg / L) releases rhubarb extract; and Level 3 response (e.g., dissolved oxygen 4.2 mg / L) deploys a flexible isolation net. The response delay is ≤1 minute, which is 10 times faster than manual emergency response. Thirdly, the system precisely constructs an emergency environment. Within 3 minutes, the water temperature in the emergency protection zone (5 m²) can be adjusted back by 1.5℃ and the dissolved oxygen can be increased to 5.2 mg / L. Within 10 minutes, a safe environment of "water temperature 20℃ and dissolved oxygen 7 mg / L" can be constructed, providing shelter for stressed juvenile salamanders.

[0068] Effects of Phased Environmental Challenge Training: Daily phased training combining "gradual challenge + extreme scenario pre-adaptation" significantly improves the extreme environmental tolerance of juvenile salamanders. Firstly, it raises their extreme environmental tolerance threshold. After daily training with gradients in temperature, water flow, and water quality, the juvenile salamanders' tolerance range for sudden temperature increases widens from "20-22℃" to "20-24℃," and their tolerance limit for low dissolved oxygen decreases from "4.5mg / L" to "4.2mg / L," laying the foundation for survival in extreme weather. Secondly, it fosters emergency behavior patterns. Through repeated extreme scenario pre-adaptation, juvenile salamanders can actively migrate to high dissolved oxygen areas (such as emergency protection zones) during sudden environmental changes, reducing the migration response time from the initial 5 minutes to 1 minute, avoiding passive stress-induced death. Thirdly, it provides stress damage pretreatment. Daily training allows juvenile salamanders to accumulate more stress-protective substances (such as HSP70 and glutathione), reducing oxidative damage (MDA content) in extreme environments by 40% compared to the "no pre-adaptation" group, thus reducing organ damage caused by extreme stress.

[0069] The segmented bionic feed and the environmental challenge deep synergy technology effect: the segmented bionic feed of "daily nutrition strengthening + extreme stress feed auxiliary" and the extreme environmental challenge form the synergy of "basic resistance - emergency protection": first, the daily feed builds the resistance foundation, the Artemia salina period feed Artemisia argyi and Radix Isatidis extract and Bacillus subtilis can enhance the daily immunity and resistance capacity, so that the Artemia salina has a higher stress tolerance basis before the extreme environment (such as the lysozyme activity is maintained above 50 U / mL); second, the feed auxiliary sedation in extreme stress, the emergency response simultaneously feeds the stress feed (containing 0.5% gamma-aminobutyric acid), which can quickly reduce the stress activity of Artemia salina (the swimming frequency is reduced from 60 times / min to 30 times / min), reduce energy consumption and collision damage; third, the feed accelerates the repair after the emergency, the recovery feed (containing 5% fish oil) is fed after the extreme environment, which can accelerate the repair of liver cells and gill tissue (the gill filament damage rate is reduced by 50% compared with the "no recovery feed" group), avoiding the long-term growth lag caused by extreme stress.

[0070] The synergistic effect is beyond expectation The technical effect: the synergy of the system's rapid emergency, the pre-adaptation of training and the auxiliary protection of feed produces the unexpected effect of "extreme environment mortality drop + stress recovery fast": first, the mortality is greatly reduced, the mortality of the "system + training + feed" synergy group in the extreme rainstorm simulation scene is only 5%, which is reduced by 84% compared with the "system not enabled" group (32%) and reduced by 72% compared with the "system only without training" group (18%), proving that the synergistic effect can maximize the survival probability in extreme environment; second, the stress impact is quickly eliminated, the behavior abnormality duration of the Artemia salina in the synergy group is only 1.5 hours, which is shortened by 75% compared with the "system not enabled" group (6 hours), and the feeding and growth recover normally after 24 hours, avoiding the "long-term growth inhibition" caused by extreme stress (the growth inhibition after traditional breeding extreme stress is 15-20%); third, the system reliability is enhanced, the synergy makes the "fault tolerance rate" of the system emergency response increase - even if there is a small delay (such as 1 minute) in the system parameter regulation, the Artemia salina trained and assisted by feed can still be tolerated, avoiding the risk of "system failure means large-scale death", providing a stable solution for extreme weather protection in large-scale breeding.

[0071] The above examples prove that the present application significantly improves the temperature tolerance, hypoxia adaptation, immune defense and other comprehensive resistance capacity of Artemia salina by designing environmental challenge training without reducing the survival rate, while ensuring that the growth performance is not affected through intelligent regulation and nutrition synergy, providing a solution for large-scale breeding and release of Artemia salina.

[0072] The above shows and describes the basic principles, main features and advantages of the present application. Those skilled in the art should understand that the present application is not limited to the above embodiments, and the above embodiments and descriptions in the specification are only to illustrate the principles of the present application. Without departing from the spirit and scope of the present application, various changes and improvements can be made to the present application, and these changes and improvements all fall within the scope of the present application. The scope of protection of the present application is defined by the appended claims and their equivalents.

Claims

1. A method for raising juvenile salamanders using an eco-friendly approach, characterized in that, Includes the following steps: Step (1) Construct a dynamic environment simulation system: Water quality, light, and temperature parameters are monitored in real time by multi-parameter sensors, and the equipment is adjusted by a fuzzy PID controller to maintain the water temperature at 18-22℃, dissolved oxygen at 5-8mg / L, and pH at 6.8-7.5, simulating the seasonal light cycle; The system is designed with biomimetic rock formations to form a cascading water flow, and the bottom of the aquaculture pond is laid with a 15-20cm pebble layer and a 1-2cm quartz sand layer, and a counter-current biological filter bed is configured; Step (2) Implement phased environmental challenge training: The challenge intensity is dynamically adjusted based on physiological indicators such as the juvenile salamander's heart rate and respiratory rate. When any indicator exceeds the baseline value by 50%, the intensity is reduced. Specifically, this includes: a. Temperature stress training: Start with ±0.5℃, and increase the temperature fluctuation range by 0.2℃ each week until it is stabilized at ±1.5℃; in summer, raise the temperature from 20℃ to 22℃ at a rate of 0.5℃ / h from 14:00 to 16:00 every day, and in winter, lower the temperature from 15℃ to 13℃ three times a week at a rate of 0.3℃ / h. b. Water flow disturbance training: Initial flow velocity 0.05 m / s, increasing by 0.02 m / s to 0.15 m / s per month, and activating a "peak flow mode" of 0.12 m / s for 1 hour every 3 days; monitoring the distribution of juvenile salamanders through underwater cameras, and automatically adjusting the angle of the deflector to change the water flow distribution when the density in a certain area is >30 salamanders / m². c. Water quality fluctuation training: Twice a month, use food-grade kaolin suspension with a particle size of 5-10μm to increase the turbidity from 5NTU to 15NTU and maintain it for 4 hours. Every two weeks, use 0.1mol / L sodium bicarbonate-citric acid buffer to make the pH fluctuate between 6.8 and 7.

2. Step (3) Segmented biomimetic feed feeding: Juvenile salamanders aged 0-90 days: Feed them a mixture of earthworm protein, mealworm pupae powder, and 1×10 7 -5×10 7 Microencapsulated feed composed of CFU / g Lactobacillus rhamnosus YFI-6; Juvenile salamanders aged 91-180 days: Feed them with a crude drug concentration of 1g / mL Artemisia annua-Isatis indigotica compound extract and 5×10 6 -1×10 7 CFU / g Bacillus subtilis extruded feed; The protein content of the biomimetic feed is 45-55% for juvenile salamanders and 40-50% for young salamanders, while the fat content is controlled at 8-12%. Step (4) Supporting Measures: Nutritional regulation: For the first 3 days of the challenge, feed the animal a stress diet containing 1% glutamine, 0.5% γ-aminobutyric acid and 0.3% nucleotides. For the last 5 days after the challenge, feed the animal a recovery diet containing 5% fish oil, 0.2% vitamin C and 2% spirulina powder. Intelligent monitoring and emergency response: Deploy a five-dimensional monitoring network that combines machine vision and LSTM neural networks. When parameters exceed the safety threshold by 80%, an emergency response is initiated, including releasing a flexible isolation net and restoring environmental parameters to 90% of the baseline value within 3 minutes. The stocking density of juvenile salamanders is 30-50 salamanders / m² for juveniles and 15-25 salamanders / m² for young salamanders, with independent control in different zones.

2. The method for raising juvenile salamanders in an eco-friendly manner according to claim 1, characterized in that: In the temperature stress training, 50 mg / kg L-cysteine ​​and 200 mg / kg vitamin E were added to the feed to induce the expression of heat shock protein HSP70.

3. The method for raising juvenile salamanders in an eco-friendly manner according to claim 1, characterized in that: The water flow disturbance training, through velocity gradient partitioning and dynamic allocation, increased the swimming speed of juvenile salamanders from 0.08 m / s to 0.15 m / s after 90 days of training.

4. The method for raising juvenile salamanders in an eco-friendly manner according to claim 1, characterized in that: In the water quality fluctuation training, sodium bicarbonate-citric acid buffer solution was used to control the pH fluctuation amplitude to ≤ ±0.3, and turbidity was adjusted using kaolin suspension.

5. The method for raising juvenile salamanders in an eco-friendly manner according to claim 1, characterized in that: The emergency response includes deploying a flexible safety net.

6. The method for raising juvenile salamanders in an eco-friendly manner according to claim 1, characterized in that: The biomimetic rock formations of the dynamic environment simulation system create a cascading water flow, with the stream velocity controlled at 0.1-0.3 m / s, in conjunction with a ground source heat pump and PCM combined device.

7. The method for raising juvenile salamanders in an eco-friendly manner according to claim 1, characterized in that: The microcapsule encapsulation wall material of the biomimetic feed is a sodium alginate-chitosan composite membrane with an encapsulation rate of ≥90% and a sustained release rate of ≤10% within 2 hours in the acidic gastric environment of juvenile salamanders. The preparation method of the Artemisia annua-Isatis tinctoria compound extract is as follows: Artemisia annua and Isatis tinctoria are mixed in a 1:1 ratio, 8 times the volume of 70% ethanol is added, and the mixture is ultrasonically extracted 3 times at 50℃ for 30 minutes each time. The extracts are combined and concentrated to a crude drug concentration of 1g / mL. The protein content of the biomimetic feed is 45-55% for juvenile salamanders and 40-50% for young salamanders, while the fat content is controlled at 8-12%.

8. The method for raising juvenile salamanders in an eco-friendly manner according to claim 1, characterized in that: The stocking density of juvenile salamanders is 30-50 salamanders / m² for juveniles and 15-25 salamanders / m² for young salamanders, with independent control in different zones.

9. The method for raising juvenile salamanders in an eco-friendly manner according to claim 1, characterized in that: The five-dimensional monitoring network combines machine vision with LSTM neural networks.

10. The method for raising juvenile salamanders in an eco-friendly manner according to claim 1, characterized in that: The bottom of the aquaculture pond is covered with a 15-20cm layer of pebbles and a 1-2cm layer of quartz sand, and a counter-current biological filter bed is configured to purify the water.

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