A soothing composition, uses and skin care

Abstract

The application belongs to the technical field of skin care, and discloses a soothing composition, an application and a skin care product. The soothing composition comprises 65-85 parts of avocado oil, 5-15 parts of bilberry seed oil, 10-20 parts of eggfruit oil and 10-20 parts of cabbage seed oil in terms of weight parts. The soothing composition provided by the application can produce a synergistic effect to improve the hyaluronidase inhibition rate of the composition by compounding avocado oil, bilberry seed oil and eggfruit seed oil, and effectively achieves the skin soothing effect.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of skin care, in particular to a soothing composition, use and skin care product. BACKGROUND

[0002] For modern skin care products, they are mainly divided into synthetic skin care products and natural plant skin care products. The synthetic skin care products have many components, mainly rely on their precise addition of specific active ingredients synthesized in the laboratory, and achieve rapid and effective skin care effect through targeted skin pathway action. For example, the prior art 1: Chinese patent application 201810309514.3 discloses a synthesis method of biological antioxidant for skin care emulsion, which uses synthetic skin care components to achieve skin antioxidant effect.

[0003] Unlike synthetic skin care components, plant oils play an indispensable role in the field of skin care, especially in soothing the skin. The mechanism of action is completely different from that of synthetic skin care products, and it shows unique advantages of natural, multi-effect and deep level. First, the core soothing effect of plant oil comes from its excellent ability to repair and consolidate the skin barrier. The human skin barrier is mainly composed of sebum film and intercellular lipids in the stratum corneum, and many components of plant oils, such as linoleic acid and linolenic acid, are very similar to the composition of human sebum. When the skin barrier is damaged due to dryness, external stimulation or excessive cleaning, these oils can effectively penetrate and fill the gaps in the lipid bilayer like "cement", rebuild a complete protective layer, thereby reducing water loss and resisting the invasion of external allergens and irritants, and relieving dryness, tightness and sensitivity from the root.

[0004] However, there are thousands of existing plant oils, and the combination of some plant oils may even produce antagonistic effect. It is a big problem to how to reasonably use the natural active ingredients in plant oils to achieve better skin soothing effect. SUMMARY

[0005] One of the purposes of the present application is to provide a soothing composition, which is formed by the combination of avocado oil, bilberry seed oil and eggfruit seed oil, so as to form a synergistic effect of improving the soothing effect of the composition, and fully utilize the advantages of plant oils to achieve excellent skin soothing effect.

[0006] Another purpose of the present application is to provide a use of the soothing composition, which is used for preparing a skin care product to make the skin care product have skin soothing effect.

[0007] Meanwhile, the present application also provides a skin care product containing a certain amount of soothing composition, which has skin soothing effect.

[0008] To achieve the above object, the present application provides a soothing composition, comprising 65-85 parts by weight of avocado oil, 5-15 parts by weight of bilberry seed oil, and 10-20 parts by weight of eggfruit oil.

[0009] Preferably, the soothing composition of the present application further comprises 10-20 parts by weight of kale seed oil.

[0010] The present application also provides the use of the soothing composition described above for preparing a skin care product.

[0011] A skin care product containing 1-50 wt% of the soothing composition described above.

[0012] Beneficial effects

[0013] Compared with the prior art, the present application provides a soothing composition, which can produce a synergistic effect of improving the hyaluronidase inhibition rate of the composition by compounding avocado oil, bilberry seed oil and eggfruit seed oil, and effectively achieve the skin soothing effect. DETAILED DESCRIPTION

[0014] The present application will be further described below in conjunction with examples, but does not constitute any limitation on the present application, and any limited modifications made within the scope of the claims of the present application are still within the scope of the claims of the present application.

[0015] In order to explain the technical content of the present application in detail, the following further describes the embodiments.

[0016] In the following examples and comparative examples, the avocado oil is purchased from Shanghai Bosike Industry Co., Ltd.; the bilberry seed oil, eggfruit seed oil and kale seed oil are all purchased from GQ Chemical Technology (Guangzhou) Co., Ltd.

[0017] The compositions are configured according to the formula table in Table 1, and the configuration method is stirring and mixing.

[0018] Table 1 Formula table of soothing composition

[0019] Avocado oil Bilberry seed oil Cupuacu seed oil Cabbage seed oil Example 1 75 10 15 0 Example 2 65 15 20 0 Example 3 85 5 10 0 Example 4 75 10 15 10 Example 5 75 10 15 20 Example 6 75 10 20 0 Comparative Example 1 75 10 0 0 Comparative Example 2 0 0 15 0 Comparative Example 3 0 0 15 10

[0020] Performance test

[0021] I. Skin soothing effect test

[0022] (I) Test basis

[0023] GDCTC / OT-L014 "Cosmetics - Hyaluronidase Inhibition Test Procedure".

[0024] References:

[0025] [1] Sun, P. D., Liu, C., & Wang, X. H. Inhibitory effect of yuzhu flower on hyaluronidase [J]. Chemical Industry Times, 2013, 76(9);

[0026] [2] Ma, X. T., Shao, S., Zhang, R. R., et al. Extraction and separation of active fraction of Chinese yam and preliminary study on its whitening activity [J]. Times Journal of Chinese Medicine, 2021, 32(10): 2388-2392;

[0027] [3] Cheng, S. J. Cosmetic evaluation alternative method standard implementation guide. China Inspection and Testing Press China Standard Press, Mar, 2017: 316-317;

[0028] [4] Takahashi T, Ikegami-Kawai M, Okuda R, et al. A fluorimetric Morgan-Elson assay method for hyaluronidase activity [J]. Analytical Biochemistry, 2003, 322(2): 257-263.

[0029] (2) Purpose and basic principles of the test

[0030] Hyaluronidase can hydrolyze hyaluronic acid, reduce the viscosity of intercellular substance, and promote the subcutaneous infusion, local accumulation of exudate or blood to speed up, which is an important participating enzyme in human skin allergic reaction. In this experiment, the in vitro inhibitory effect of the test sample on hyaluronidase was studied to evaluate the soothing and anti-allergic effect; hyaluronidase can hydrolyze the 1,4-bond between β-N-acetylglucosamine and D-glucuronate in hyaluronic acid without selectivity, and β-N-acetylglucosamine is obtained. When β-N-acetylglucosamine is heated with dilute alkali, the aldehyde group and acetyl group react to form a pyrrole derivative, which is condensed with p-dimethylaminobenzaldehyde in an acidic solution to produce a strong purple red color. By measuring the absorbance of the final reaction solution at a specific wavelength, the inhibitory effect of the test sample on hyaluronidase can be calculated according to the calculation formula.

[0031] (3) Test items

[0032] 1. Soothing effect in cosmetic efficacy evaluation (laboratory method): cosmetic-hyaluronidase inhibition

[0033] 2. Judgment criteria

[0034] 2.1 If the hyaluronidase inhibition rate of the test sample is greater than that of the negative control, it is considered to have significant difference;

[0035] 2.2 If the hyaluronidase inhibition rate of the test sample is less than that of the negative control, it is considered to have no significant difference;

[0036] 2.3 The IC50 of glycyrrhizin dipotassium salt should be between 1.75 mg / mL and 2.25 mg / mL, otherwise the experiment is invalid.

[0037] (Four) Laboratory environment

[0038] Temperature: 25℃; relative humidity: 50%.

[0039] (Five) Test method

[0040] 1. Reagents: positive control: glycyrrhizin dipotassium salt; sodium acetate; glacial acetic acid; hyaluronidase; sodium hyaluronate; calcium chloride; sodium carbonate; p-dimethylaminobenzaldehyde; dimethyl sulfoxide.

[0041] 1. Reagents: positive control: glycyrrhizin dipotassium salt; sodium acetate; glacial acetic acid; hyaluronidase; sodium hyaluronate; calcium chloride; sodium carbonate; p-dimethylaminobenzaldehyde; dimethyl sulfoxide.

[0042] 2. Instruments: electronic balance, ultraviolet spectrophotometer, pipette.

[0043] 3. Test steps

[0044] 3.1 Preparation before testing: dissolve and dilute the positive control with water to a series of concentration gradients: 2.50 mg / mL, 2.25 mg / mL, 2.00 mg / mL, 1.75 mg / mL, 1.50 mg / mL to verify the test system.

[0045] 3.2 Sample processing: weigh the sample and perform extraction and pretreatment on the sample to make the concentration of the sample 20% (primary water as negative control).

[0046] 3.3 Measurement

[0047] Take 0.1 mL of calcium chloride solution and 0.5 mL of hyaluronidase solution and incubate at 37℃ for 20 min; add 0.5 mL of sample diluent and continue incubation at 37℃ for 20 min; add 0.5 mL of sodium hyaluronate solution and incubate at 37℃ for 30 min, and place at room temperature for 5 min; add 0.1 mL of sodium carbonate solution and place in a boiling water bath for 10 min, then immediately cool with ice water; add 1 mL of p-dimethylaminobenzaldehyde reagent, and finally dilute to 5 mL with glacial acetic acid, stand for color development for 45 min, and transfer each reaction solution into a 1 cm cuvette, and measure the absorbance value at 583 nm using an ultraviolet spectrophotometer. (Note: when the sample formulation does not contain hyaluronic acid salt, the measurement of tubes E and F is not required).

[0048] 3.4 Calculation

[0049] .

[0050] Wherein:

[0051] A - Control solution ABS value (hyaluronidase + deionized water + sodium hyaluronate);

[0052] B - Control blank solution ABS value (buffer solution + deionized water + sodium hyaluronate);

[0053] C - Sample solution ABS value (hyaluronidase + sample + sodium hyaluronate);

[0054] D - Sample blank solution ABS value (buffer solution + sample + sodium hyaluronate);

[0055] E - Sample solution ABS value (hyaluronidase + sample + buffer solution);

[0056] F - Sample blank solution ABS value (buffer solution + sample + buffer solution);

[0057] The Guangdong Province Baohua Detection Center Co., Ltd. was commissioned to detect Examples 1-6 and Comparative Examples 1-3 according to the above method, and the results are shown in Table 2; wherein the report number of Example 1 is E202501448b, the report number of Example 2 is E202501449b, the report number of Example 3 is E202501450b, the report number of Example 4 is E202501451b, the report number of Example 5 is E202501452b, the report number of Comparative Example 1 is E202501453b, the report number of Comparative Example 2 is E202501454b, the report number of Comparative Example 3 is E202501455b, and the report number of Example 6 is E202501552a. It should be noted that Example 6 is a test for further verification of the synergistic effect of the composition according to the results of Examples 1-5 and Comparative Examples 1-3, so it is a subsequent commissioning of a detection agency, and the report number is detached from Examples 1-5 and Comparative Examples 1-3.

[0058] Table 2: Hyaluronidase inhibition rate test results of soothing composition of Examples 1-6 and Comparative Examples 1-3

[0059] Hyaluronidase inhibition rate Example 1 22.9 Example 2 26.4 Example 3 20.1 Example 4 20 Example 5 23 Example 6 23.1 Comparative Example 1 14 Comparative Example 2 9 Comparative Example 3 10 Negative control 0.7

[0060] According to the results in Table 2, it can be seen that:

[0061] According to the data of Examples 1-6, the composition of the present application has a high hyaluronidase inhibition rate at a concentration of 20%, and has a good skin soothing effect; wherein according to the comparison of the data of Example 4, Example 5 and Example 1, adding a certain amount of kale seed oil on the basis of the compounding of avocado oil, cranberry seed oil and eggfruit seed oil can effectively improve the hyaluronidase inhibition rate of the composition, but if the amount of kale seed oil added is small as in Example 4, it will affect the hyaluronidase inhibition rate of the composition.

[0062] Meanwhile, according to the data analysis of Examples 1-3, the hyaluronidase inhibition rates of the compositions of Examples 1-3 have great differences, which is presumably due to the use amount of certain components in the compositions. According to Table 1, it can be presumed that:

[0063] (1) The concentration of the blueberry seed oil affects the hyaluronidase inhibition rate of the composition;

[0064] (2) The concentration of the eggfruit seed oil affects the hyaluronidase inhibition rate of the composition;

[0065] However, according to the data analysis of Example 6, the increase in the concentration of the eggfruit seed oil does not significantly increase the hyaluronidase inhibition rate of the composition.

[0066] According to the comparison of the data of Example 1 and Comparative Example 1, the use of avocado oil and blueberry seed oil alone can significantly reduce the hyaluronidase inhibition rate of the composition at a concentration of 20%. According to the data of Comparative Example 2, the eggfruit seed oil itself does not have obvious skin soothing effect, and its hyaluronidase inhibition rate is only 9%.

[0067] Therefore, it can be calculated that if the composition of the present application is only the superposition of the effects of the components, according to the data of Comparative Example 1 and Comparative Example 2, the theoretical hyaluronidase inhibition rate of Example 1 should be 14*0.85+9*0.15=13.25. However, the actual hyaluronidase inhibition rate of Example 1 is 22.9, which is 172.8% of the theoretical value, indicating that the combination of avocado oil, blueberry seed oil and eggfruit seed oil produces a synergistic effect of improving the skin soothing effect of the composition.

[0068] According to the data of Comparative Example 3 and the data of Comparative Example 2, it can be presumed that the skin soothing effect of the cabbage seed oil and the eggfruit seed oil is similar, and the hyaluronidase inhibition rate of the cabbage seed oil is slightly higher than that of the eggfruit seed oil. However, according to Examples 4 and 5 of the present application, it can be found that the increase in the amount of cabbage seed oil based on the combination of avocado oil, blueberry seed oil and eggfruit seed oil can significantly increase the hyaluronidase inhibition rate of the composition, indicating that the addition of cabbage seed oil based on the combination of avocado oil, blueberry seed oil and eggfruit seed oil has a certain degree of synergistic effect.

[0069] II. Moisturizing effect

[0070] (I) Test basis

[0071] GDCTC / OT-L012 "Test Method for Moisturizing Efficacy (Physical Method) - Procedure" (Method 2).

[0072] Reference:

[0073] [1] Wang, B. M., Zhu, J. J., Gong, J. R. Influence of Common Oils on the Moisturizing Effect of Cosmetics[J]. Perfume & Flavorist, 2017(4): 6.

[0074] (2) Purpose and basic principle of the test

[0075] Different moisturizing agents in the product have different effects on water molecules, and their ability to absorb and retain water also differs slightly. Oil has a sealing effect on water, which can prevent water loss. The better the sealing, the less water loss.

[0076] (3) Test items

[0077] 1. Moisturizing efficacy (physical method) test.

[0078] 2. Judgment criteria:

[0079] If the sample's moisturizing rate is higher than the blank control and the water loss rate is lower than the blank control, it is considered that the sample has a moisturizing effect under the test conditions.

[0080] (4) Laboratory environment

[0081] Temperature: 40℃; Relative humidity: 65%.

[0082] (5) Test method

[0083] 1. Test design

[0084] 1.1 Weigh about 20g (accurate to 0.0001g) of water (m0) in a weighed bottle (or other test bottle), place a quick filter paper on the bottle, and fix the filter paper on the bottle opening. The sample to be tested is evenly applied on the filter paper until the filter paper is completely wet, then placed in a constant temperature and humidity machine at (40±2)℃, relative humidity (65±5)% for n hours (n is generally 1, 2, 4, 8 hours, the actual test time is arranged according to the demand). After n hours, take the weight (mn).

[0085] 1.2 Except for the sample without coating, follow the above method to do the blank test.

[0086] 1.3 Three parallel tests, take the average value, calculate the moisturizing rate and water loss rate of the sample after n hours, and draw the change curve.

[0087] 1.4 Judgment criteria

[0088] If the sample has a higher moisture content and a lower water loss rate than the blank control, it is considered to have a moisturizing effect under the test conditions.

[0089] 2. Test object:

[0090] 2.1 Physical state: yellow transparent liquid;

[0091] 2.2 Preparation method: as is.

[0092] 3. Instrument reagent:

[0093] 3.1 Instrument: constant temperature and humidity machine, electronic balance;

[0094] 3.2 Reagent: water.

[0095] The Guangdong Province Health Inspection Center Co., Ltd. was commissioned to test Examples 1-6 and Comparative Examples 1-3 according to the above method, and the results are shown in Table 3.

[0096] Table 3 Moisturizing rate test of soothing composition of Examples 1-6 and Comparative Examples 1-3

[0097] 1 h moisturizing rate 2 h moisturizing rate 4 h moisturizing rate 8 h moisturizing rate Example 1 99.1 98.5 96.6 94.1 Example 2 99.2 98.8 96.6 94.2 Example 3 99.3 99.0 97.1 94.7 Example 4 99.3 98.9 96.6 93.9 Example 5 99.3 98.5 97.0 94.2 Example 6 98.7 97.9 95.3 92.4 Comparative Example 1 99.4 98.4 96.8 94.3 Comparative Example 2 99.0 97.8 96.0 92.9 Comparative Example 3 99.1 97.7 95.6 92.7 Blank control 97.3 95.6 89.4 82.9

[0098] According to the results in Table 3, it can be seen that:

[0099] Examples 1-6 and Comparative Examples 1-3 all have good moisturizing effect.

[0100] III. Repair effect

[0101] 1. Test basis

[0102] The test was designed according to "RUBY / MS-s0038-A00".

[0103] 2. Test purpose and principle

[0104] 2.1 Purpose of the test

[0105] By comparing the effect of the sample on the migration rate of HaCaT cells, the repair efficacy of the sample was explored.

[0106] 2.2 Test principle

[0107] Skin is the largest organ of the human body, consisting of epidermis, dermis, and subcutaneous fat layer. The outermost layer of the skin is the stratum corneum, which is mainly composed of multiple layers of pavement stone-like keratinocytes. The cells are rich in keratin, which plays a protective role in the skin. If there is a superficial wound on the surface of the skin, the epidermal cells, i.e., keratinocytes, need to be regenerated to quickly heal the wound and reduce the formation of scars. When the cells grow to a single layer in vitro, a blank area is artificially created on the single layer of cells. The cells at the edge of the scratch will gradually enter the blank area to heal the scratch, which to some extent simulates the process of cell migration in vivo. Therefore, by measuring the cell migration rate after sample treatment, it is evaluated whether the sample has a repair effect.

[0108] 3. Test material

[0109] 3.1 Test cells

[0110] The cell line used in this test is a human immortal keratinocyte cell line (HaCaT).

[0111] 3.2 Main instruments and reagents

[0112] 3.2.1 Main test instruments: CCL-050B-carbon dioxide cell incubator, TGL-16 medical centrifuge, SW-CJ-2FD super clean bench, Leica inverted microscope.

[0113] 3.2.2 Main test reagents: high-sugar DMEM medium, phosphate buffer solution (PBS), 10% fetal bovine serum, trypsin.

[0114] 4. Test method

[0115] 4.1 Grouping and control

[0116] Product dose grouping: HaCaT cells treated with a sample having a concentration of 0.0156%;

[0117] Blank control group: HaCaT cells normally cultured before use of the sample.

[0118] 4.2 Main operation steps

[0119] 4.2.1 Cell culture

[0120] The frozen HaCaT cells were inoculated into cell culture bottles at an appropriate density, and DMEM medium (10% FBS) was added. The culture was carried out in a cell incubator at 37°C and 5% CO2.

[0121] 4.2.2 Cell plating

[0122] After the HaCaT cells in logarithmic growth phase were washed twice with PBS, trypsin was added for digestion, and the digestion was terminated by adding DMEM medium (10% FBS) and centrifugation. The supernatant was discarded, and DMEM medium (10% FBS) was added to prepare a cell suspension. 2 mL of the cell suspension was added to each well of a 6-well plate, which was then incubated in a cell incubator.

[0123] 4.2.3 Study on sample promoting HaCaT cell migration activity

[0124] After the cell scratch was constructed (simulating a skin wound), PBS was washed, 2 mL of serum-free DMEM medium was added to the blank control group, and 2 mL of serum-free DMEM medium containing the sample at a concentration of 0.0156% was added to the sample group. After 72 h, the same field of view of the sample group was photographed and recorded, and the scratch distance (d) at three positions at this time point was measured to calculate the cell migration rate and the relative cell migration rate.

[0125] Cell migration rate (%) = (d 0h -d xh ) / d 0h x 100%;

[0126] Relative cell migration rate of the sample group (%) = (cell migration rate of the sample group - cell migration rate of the blank group) / cell migration rate of the blank group x 100%.

[0127] The Guangdong Provincial Institute for Food and Drug Control Co., Ltd. was commissioned to detect Example 1 according to the above method, and the results are shown in Table 4.

[0128] Table 4: Results of Example 1 composition repair efficacy test

[0129] Group Blank control group Example 1 Cell migration rate 9.03 18.64

[0130] According to the results in Table 4, it can be seen that:

[0131] The scratch test in vitro is used to evaluate the migration ability of cells, and the ability of the sample to promote cell migration is evaluated according to the healing degree of the scratch wound.

[0132] After the HaCaT cells were treated with Example 1 at a concentration of 0.0156% for 72 h, the cell migration rate was 18.64%, which was increased by 9.61% compared with the blank control group. Further calculation of the relative migration rate showed that after the HaCaT cells were treated with Example 1 at a concentration of 0.0156% for 72 h, the cell migration rate was increased by 106.42% compared with the blank control group, indicating that Example 1 at a concentration of 0.0156% had a significant effect on promoting HaCaT cell migration.

[0133] The embodiments presented herein are only examples selected from combinations of all possible embodiments. The appended claims should not be limited to the embodiments described herein. Subranges of the ranges mentioned in the claims are also intended to be covered.

Claims

1. A soothing composition, characterized in that, By weight, it includes 65-85 parts avocado oil, 5-15 parts blueberry seed oil, 10-20 parts passion fruit oil, and 20 parts kale seed oil.

2. Use of the soothing composition as described in claim 1 in the preparation of skin care products.

3. A skincare product, characterized in that, Contains 1-50 wt% of the soothing composition according to claim 1.