Modeling device for mouse skin photoaging individualized flexible fixation and eye protection and stress intervention

Abstract

The invention discloses an integrated experimental device for mouse skin photoaging modeling, and relates to the technical field of skin photoaging modeling. The mouse fixing device comprises a fixing main plate and a plurality of semicircular fixing cylinders. The fixed main board is erected in the experiment box body and is provided with a plurality of groups of fixed cylinder slots; the semicircular fixed cylinder is arranged in the fixed cylinder slot in a pluggable manner; a cylinder body of the semicircular fixing cylinder is provided with a fixing hole through which an elastic binding piece penetrates, and the head end of the semicircular fixing cylinder is provided with an integrally-formed arc-shaped baffle used for shielding the eyes of the mouse during ultraviolet irradiation. The stress intervention system is integrated in the experiment box body and comprises a blue light phototaxis sedation module and an olfaction pacification spraying module; the blue light phototaxis sedation module comprises at least one group of blue light sources arranged in the box body and is used for inducing mice to settle; the olfaction pacifying spraying module comprises an atomizer and a spraying outlet and is used for releasing pacifying aerial fog into the box body, multiple functions of animal fixing, ultraviolet irradiation, eye protection and stress control are synchronously completed in a single device, and the standardization degree and data reliability of light aging modeling are remarkably improved.

Description

Technical Field

[0001] This invention relates to the field of animal experimental equipment technology, and in particular to a multifunctional mouse skin photoaging modeling device that integrates individualized flexible fixation, ultraviolet irradiation, eye protection, and behavioral stress intervention. Background Technology

[0002] Skin aging caused by environmental factors is collectively referred to as extrinsic aging. Ultraviolet radiation is the most common environmental factor. Photoaging is a major cause of skin aging. In exploring methods to delay photoaging and developing drugs to combat it, the construction of photoaging model mice is crucial, and the stability and standardization of the model directly determine the reliability of the experimental results.

[0003] A common method for establishing photoaging models involves repeatedly irradiating the skin on the backs of mice with UVA and UVB rays. However, if freely moving mice are directly irradiated with a lamp, they will move, avoid the light, or even pile up on each other due to their natural instincts. This results in uneven UV radiation doses received by each individual, significant individual differences, unstable modeling effects, and compromised experimental accuracy. Therefore, it is essential to effectively restrict the movement of the mice during irradiation to ensure that their backs are continuously and evenly exposed to ultraviolet light.

[0004] In addressing the aforementioned challenges, existing technologies have primarily developed two approaches, but neither has systematically solved the problem: First, the "spatial confinement method." This method physically separates mice using partitions or individual placement cells to prevent aggregation. However, this only achieves separation of activity areas; mice can still move, turn, or curl up freely within their respective spaces, failing to guarantee uniform and continuous exposure of their back skin posture, thus making it difficult to fundamentally guarantee irradiation uniformity. Second, the "mechanical hard confinement method." This method actively compresses the mice's activity space through complex linkage mechanisms to forcibly expose their backs. While improving the exposure problem to some extent, it is structurally complex, costly, prone to malfunctions, and presents cleaning challenges. More importantly, this forced mechanical constraint easily induces strong stress responses in mice, introducing new experimental variables that interfere with their normal physiological state and the reliability of experimental data.

[0005] Furthermore, a critical issue that is often overlooked is eye protection. None of the aforementioned technical approaches include effective eye protection structures, exposing mice to the risk of high doses of ultraviolet radiation. This not only violates animal experimental ethics but also leads to abnormal behavior and physiological indicators due to visual impairment, further weakening the reliability of experimental data.

[0006] In summary, existing technologies suffer from systemic deficiencies in three key dimensions: reducing animal stress, achieving individualized and stable fixation, and providing effective eye protection, thus failing to meet the demands of high-precision photoaging modeling. Therefore, there is an urgent need in this field for an innovative device capable of addressing all of the aforementioned problems in a coordinated manner. Summary of the Invention

[0007] In the embodiments of the present invention, the technical solutions involved will be systematically and comprehensively described with reference to the accompanying drawings. It should be noted that the embodiments listed herein are only some specific implementations and do not cover all possible implementations of the present invention.

[0008] The purpose of this invention is to overcome the shortcomings of existing technologies and provide a device for modeling photoaging of mouse skin. This device not only enables individualized flexible fixation and uniform irradiation for each mouse, but also innovatively integrates a unified eye protection and multi-sensory stress intervention system, thereby ensuring standardization of the modeling process and accuracy of data at both physical and physiological / psychological levels. To achieve the above objectives, this invention adopts the following technical solution: An individualized flexible fixation device for modeling photoaging of mouse skin, characterized in that it includes a box, a fixed main board, multiple semi-circular fixation cylinders, and a stress intervention system; The top of the enclosure has an opening and is fitted with a lid. The lid has a mounting position for an ultraviolet lamp. The wavelength range of the ultraviolet lamp source is 280-400 nm, and the irradiation intensity range is 0.5-2.0 mW / cm². Specifically, in this embodiment, a wavelength of 313 nm and an irradiation intensity of 0.9 mW / cm² were selected. 2 The ultraviolet lamp source; the front of the enclosure is provided with a transparent observation window; The fixed base is made of two acrylic plates spliced ​​together, one of which has multiple equally spaced fixing cylinder slots. The semi-circular fixing cylinder is detachably installed in the fixing cylinder slot to achieve independent fixation of each mouse without interference; the body of the semi-circular fixing cylinder is provided with fixing holes for threading a flexible fixing strap. The head end of the semi-circular fixing cylinder is provided with an integrally formed arc-shaped baffle, which constitutes an eye protection structure to completely block the mouse's eyes during ultraviolet radiation and avoid vision damage. The stress intervention system includes: 1) Blue light phototactic sedation module: at least one set of blue light sources with a wavelength of 480 nm are set on the inner wall of the side panel of the experimental chamber. The light emitted does not directly irradiate the modeling area on the back of the mouse, and the wavelength is configured to have a sedative phototactic effect on the mouse. 2) Olfactory Soothing Spray Module: This module includes an atomizer and a spray outlet. The spray outlet is located inside the experimental chamber and is used to release a calming aromatherapy mist into the chamber environment. The atomizer has a spray particle size range of 1-10 μm and a spray rate range of 0.1-0.5 mL / min. Specifically, in this embodiment, we selected an atomizer with a spray particle size of 5 μm and a spray rate of 0.2 mL / min. The calming aromatherapy mist is made of lavender essential oil.

[0009] Furthermore, the box body has multiple screw holes of different heights on both sides for mounting hexagonal iron posts. The fixed base is mounted on the hexagonal iron posts. By adjusting the height of the base, the distance between the ultraviolet lamp and the mouse can be controlled, thereby adjusting the irradiation intensity.

[0010] Furthermore, the flexible fixing band is an elastic band with a width of 5-10mm and a thickness of 2-3mm. In particular, in a specific embodiment, we selected an elastic band with a width of 6mm and a thickness of 2mm.

[0011] Furthermore, the height adjustment mechanism includes multiple mounting screw holes on both sides of the housing and corresponding support columns. The distance between the mounting screw holes is 5cm, and the adjustable height is 5-15cm. The fixed main board is mounted on the support columns.

[0012] Furthermore, the fixing main board is made of transparent acrylic sheet. As a preferred embodiment, the fixing main board is composed of two acrylic sheets joined together, one of which has the fixing cylinder slot, and the other has a corresponding slender slot for threading the elastic band.

[0013] Furthermore, the semi-circular fixing cylinder is manufactured by 3D printing and is made of biocompatible resin.

[0014] Compared with the prior art, the present invention has the following significant advantages: (1) Individualized and precise fixation: By using a semi-circular fixation tube and a flexible fixation strap, each mouse can be fixed in an independent position, ensuring that the skin on the back is exposed in a consistent manner, thus fundamentally solving the problem of irradiation uniformity.

[0015] (2) Active stress intervention: The integrated blue light sedation and aromatherapy spray calming modules actively reduce the stress level of mice, reduce physiological data noise, and improve experimental accuracy.

[0016] (3) Integrated eye protection: The arc-shaped baffle at the head of the fixed tube can effectively block ultraviolet rays, prevent vision damage in mice, comply with animal ethics and eliminate experimental interference.

[0017] (4) Modular high-throughput design: The plug-in structure of the fixed tube and the motherboard supports simultaneous experiments with multiple mice and quick disassembly and cleaning, which significantly improves experimental efficiency.

[0018] (5) Precisely adjustable dose: By using the support column and screw hole, the distance between all mice and the ultraviolet lamp can be adjusted uniformly to achieve linear control of irradiation intensity. Attached Figure Description

[0019] Figure 1 This is a schematic diagram of the box structure in this invention; Figure 2 This is a schematic diagram of the structure of the fixing plate one in this invention; Figure 3 This is a schematic diagram of the structure of the fixing plate two in this invention; Figure 4 This is a schematic diagram of the structure of the fixed cylinder in this invention. Detailed Implementation This embodiment provides a fixed irradiator for constructing a mouse skin photoaging model, such as... Figure 1 As shown, the experimental chamber includes an experimental housing 1, a fixed base, and multiple fixed cylinders 13. The experimental housing 1 is assembled from a bottom plate 2, side plates 3, a back plate 4, a front panel 5, and a top plate 6. All the plates are made of opaque aluminum alloy to ensure the airtightness and uniformity of the internal irradiation environment.

[0020] The inner wall of side plate 3 is provided with a blue light tube mounting hole for assisting in the installation of a blue light source with a wavelength of 480nm. This blue light can effectively promote the recovery of mice from the stress state induced by experimental treatment and help regulate their endogenous endorphin levels, thereby potentially reducing their sensitivity to painful stimuli.

[0021] To further alleviate experimental stress, the device also integrates a nebulized calming system. Lavender essential oil, known for its calming properties, can be placed inside the nebulizer 7. After forming a uniform aerosol with a particle size of 5 μm, it is delivered via a gas delivery tube at a rate of 0.2 mL / min to the spray outlet 8 located on the upper left of the back plate 4. This elevated arrangement facilitates natural settling of the spray within the chamber, achieving uniform diffusion from top to bottom. A cooling fan mounting hole is provided on the lower right of the back plate 4, allowing excess gas to be expelled promptly after the calming spray program, maintaining airflow. This design also helps regulate the internal temperature of the chamber, preventing excessive temperature rise due to prolonged closed ultraviolet irradiation, thus ensuring the stability of experimental conditions and the reliability of results.

[0022] The front panel 5 features an openable and closable observation window 10 made of transparent acrylic sheet, facilitating real-time observation of the mouse's condition during the experiment. The top panel 6 has mounting holes for the ultraviolet lamp and is fitted with an openable and closable lid, allowing for irradiation at a wavelength of 313nm and an intensity of 0.9mW / cm². 2The installation, replacement, and initial adjustment of the irradiation distance of the ultraviolet lamps.

[0023] like Figure 2 As shown, the fixing base is composed of two transparent acrylic plates, Fixing Plate 1 and Fixing Plate 2. One plate has multiple equally spaced semi-circular fixing tube slots 16 for independently placing fixing tubes 13, allowing multiple mice to be fixed simultaneously without interference. The other plate has corresponding holes cut into it for threading elastic bands with a width of 6mm and a thickness of 2mm. These, along with the foreleg armpit fixing holes 18 and hind leg fixing holes 19 on the fixing tubes, flexibly fix the mouse limbs, comfortably confining the mouse inside the tubes, allowing its back skin to lie naturally flat and continuously exposed to the irradiation area. This reduces animal stress and avoids irradiation errors caused by movement.

[0024] like Figure 3 As shown, the fixing cylinder 13 is manufactured using 3D printing technology, and its length is adapted to the body length of common laboratory mice. An arc-shaped baffle 20 is provided at the front end of the cylinder, which can effectively shield the mice's eyes during irradiation, preventing ultraviolet radiation from damaging their vision. This structural design fully reflects the ethical requirements of animal experiments. To further precisely control the irradiation dose, three screw holes with a height spacing of 5cm are provided on the inner side of both side panels of the experimental chamber 1 for installing hexagonal iron pillars as supports, allowing for an adjustable height range of 5-15cm. The fixing base is mounted on the support pillars. By selecting different installation positions at different heights, the distance between the fixing base and the top ultraviolet lamp can be adjusted as a whole, thereby achieving uniform control of the irradiation intensity on the back of each mouse.

[0025] When using this device, first place the mice one by one into the fixation tube 13 made of biocompatible resin, adjust the elastic bands to properly secure the limbs, and then place the fixation tubes into the corresponding slots of the fixation base. After closing the lid, turn on the ultraviolet lamp, and the irradiation process can be monitored in real time through the front observation window. This device has a reasonable structure and is easy to assemble and disassemble, which can effectively improve the stability and experimental efficiency of photoaging modeling, and is suitable for various skin photoaging-related studies.

[0026] The above description is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any changes or substitutions that can be easily conceived by those skilled in the art within the scope of the technology disclosed in the present invention should be included within the scope of protection of the present invention.

Claims

1. A device for modeling skin photoaging of mice, comprising an experimental box (1), a fixed main plate (14, 15), a plurality of semi-circular fixed cylinders (13), and a stress intervention system, characterized in that: the fixed main plate (14, 15) is arranged inside the experimental box (1), and a plurality of fixed cylinder slots (16) are arranged on the fixed main plate (14, 15); the semi-circular fixed cylinder (13) is plug-in arranged in the fixed cylinder slot (16); the head of the semi-circular fixed cylinder (13) is provided with an integrally formed arc-shaped baffle (20) for shielding the eyes of the mice during irradiation; the cylinder body of the semi-circular fixed cylinder (13) is provided with fixing holes (18, 19) for passing through elastic restraint bands, and the elastic restraint bands are used to flexibly fix the mice; an ultraviolet light source is arranged on the top of the experimental box (1); the stress intervention system is integrated inside the experimental box (1) and comprises a blue light phototaxis sedation module (12) for inducing sedation of the mice and an olfactory soothing spray module (7, 8) for releasing soothing aerosols. The elastic restraint band is an elastic band. The width of the elastic band is 5-10 mm, and the thickness is 2-3 mm. The fixed main plate (14, 15) is formed by splicing a first plate body (14) and a second plate body (15), the first plate body (14) is provided with the fixed cylinder slot (16), the second plate body (15) is provided with an elongated slot (17) corresponding in position to the fixed cylinder slot (16), and the elastic restraint band is passed through the elongated slot (17). The wavelength range of the ultraviolet light source is 280-400 nm, and the irradiation intensity is 0.5-2.0 mW / cm². The wavelength of the blue light of the blue light phototaxis sedation module (12) is 450-490 nm.

2. The apparatus of claim 1, wherein, The olfactory soothing spray module (7, 8) comprises an atomizer (7) and a spray outlet (8), the atomizer (7) is used to atomize a plant essential oil soothing agent, the spray particle size is 1-10 μm, and the spray rate is 0.1-0.5 mL / min.

3. The apparatus of claim 2, wherein, A height adjusting mechanism is arranged on the side plate (3) of the experimental box (1), the height adjusting mechanism comprises a plurality of mounting screw holes (11) and support columns matched with the screw holes, the fixed main plate (14, 15) is arranged on the support columns, and the adjusting height is 5-15 cm.

4. The apparatus of claim 1, wherein, The semi-circular fixed cylinder (13) is integrally formed by 3D printing technology and is made of biocompatible resin.

5. The apparatus of claim 1, wherein, ​ 6. The apparatus of claim 1, wherein, ​ 7. The apparatus of claim 1, wherein, ​ 8. The apparatus of claim 1, wherein, ​ 9. The apparatus of claim 1, wherein, ​

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