Wide-field optical slice imaging method, device and equipment based on virtual illumination modulation

Abstract

The invention relates to the technical field of optical imaging, and provides a wide-field optical section imaging method based on virtual illumination modulation, and the method comprises the steps: obtaining an initial wide-field illumination image and a modulation stripe image of the initial wide-field illumination image, and carrying out the virtual illumination modulation according to the modulation stripe image and the initial wide-field illumination image, obtaining a structural illumination image corresponding to the initial wide-field illumination image, obtaining high-frequency information and low-frequency information corresponding to the initial wide-field illumination image according to the initial wide-field illumination image and the structural illumination image, and obtaining the wide-field illumination image based on weighted fusion of the high-frequency information and the low-frequency information. And obtaining a wide-field optical section tomographic image corresponding to the initial wide-field illumination image. By means of the mode, use of an optical modulation device is avoided, the wide-field optical section tomography effect is achieved only through one initial wide-field illumination image, the wide-field optical section imaging efficiency is improved, the problem that a traditional optical section tomography technology is affected by tissue scattering is solved, and the wide-field optical section imaging quality is improved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of optical imaging technology, in particular to a wide-field optical sectioning imaging method, device and equipment based on virtual illumination modulation. BACKGROUND

[0002] At present, the faller imaging structure of the wide-field fluorescence microscope will cause the fluorescence signals inside and outside the focus to be excited at the same time, so that the wide-field detection cannot effectively separate the fluorescence signals inside the focus, thereby causing the imaging image to be blurred, which seriously affects the quality of three-dimensional imaging. In order to solve this problem, the light section structure light microscope uses structured illumination technology to maintain the modulation characteristics inside the focus, and to degenerate into uniform illumination outside the focus, so as to distinguish the fluorescence signals inside and outside the focus, and realize the effect of optical tomography imaging.

[0003] In the prior art, the light section structure light imaging can be realized by combining the three-step phase shift method with the square law algorithm, or by combining the hybrid illumination with the high and low frequency filtering algorithm to achieve wide-field optical tomography imaging. However, the method based on the three-step phase shift method or the hybrid illumination requires at least two or more illumination steps, which limits the speed of the structured light imaging to some extent. In addition, the tissue scattering phenomenon will reduce the contrast of the structured light fringe, and thus weaken the optical tomography ability of the structured light microscope.

[0004] The deep learning technology can generate multiple structured illumination images with phase shift characteristics through a convolutional neural network, thereby realizing single-frame optical tomography. However, the generalization ability of deep learning is limited, and a large amount of pre-training is required for different samples, which increases the complexity of deep learning in optical tomography application. On the other hand, the HiLo algorithm based on edge detection can realize optical tomography of a single wide-field image through edge detection technology, replacing structured illumination. However, this method will cause the loss of low-frequency information inside the focus in structured illumination, thereby causing the loss of information inside the focus in the optical sectioning tomography image.

[0005] Therefore, there is an urgent need for a method that can improve the efficiency and quality of wide-field optical sectioning tomography imaging. SUMMARY

[0006] Embodiments of the present application provide a wide-field optical sectioning imaging method, device and equipment based on virtual illumination modulation. The method comprises the following steps: obtaining an initial wide-field illumination image and a modulation fringe image of the initial wide-field illumination image; performing virtual illumination modulation according to the modulation fringe image and the initial wide-field illumination image to obtain a structured illumination image corresponding to the initial wide-field illumination image. In this way, the virtual illumination modulation technology is used to replace the real illumination field modulation process, thereby avoiding the use of optical modulation devices. Furthermore, high-frequency information and low-frequency information corresponding to the initial wide-field illumination image are obtained according to the initial wide-field illumination image and the structured illumination image. Finally, a wide-field optical sectioning tomographic image corresponding to the initial wide-field illumination image is obtained based on the weighted fusion of the high-frequency information and the low-frequency information. In this way, the wide-field optical sectioning tomographic effect can be achieved only by using one initial wide-field illumination image, which not only improves the efficiency of wide-field optical sectioning imaging, but also solves the problem that the traditional optical sectioning tomographic technology is affected by tissue scattering, thereby significantly improving the quality of wide-field optical sectioning imaging.

[0007] To achieve the above-mentioned purpose, in a first aspect, embodiments of the present application provide a wide-field optical sectioning imaging method based on virtual illumination modulation, comprising: obtaining an initial wide-field illumination image and a modulation fringe image of the initial wide-field illumination image; performing virtual illumination modulation according to the modulation fringe image and the initial wide-field illumination image to obtain a structured illumination image corresponding to the initial wide-field illumination image; obtaining high-frequency information and low-frequency information corresponding to the initial wide-field illumination image according to the initial wide-field illumination image and the structured illumination image; obtaining a wide-field optical sectioning tomographic image corresponding to the initial wide-field illumination image based on the high-frequency information and the low-frequency information.

[0008] In one embodiment, the step of performing virtual illumination modulation according to the modulation fringe image and the initial wide-field illumination image to obtain a structured illumination image corresponding to the initial wide-field illumination image comprises: performing product operation on the modulation fringe image and the initial wide-field illumination image to obtain an initial structured illumination image; performing integral processing on the initial structured illumination image to obtain the structured illumination image.

[0009] In one embodiment, the initial wide-field illumination image can be defined by the following expression:

[0010] wherein, x and yrepresents the Cartesian coordinates of the image plane, and represents the Cartesian coordinates of the object plane, represents a distribution function, represents a point spread function; The initial structure illumination image can be defined by the following expression:

[0011] wherein, represents a modulated fringe image; The structure illumination image can be defined by the following expression: .

[0012] In one embodiment, the obtaining, according to the initial wide-field illumination image and the structure illumination image, high-frequency information and low-frequency information corresponding to the initial wide-field illumination image comprises: performing high-pass filtering processing on the initial wide-field illumination image to obtain the high-frequency information; obtaining spatial contrast according to the initial wide-field illumination image and the structure illumination image; obtaining the low-frequency information according to the spatial contrast and the initial wide-field illumination image.

[0013] In one embodiment, the obtaining, according to the spatial contrast and the initial wide-field illumination image, the low-frequency information comprises: obtaining a product value of the spatial contrast and the initial wide-field illumination image; performing low-pass filtering processing on the product value to obtain the low-frequency information.

[0014] In one embodiment, the high-frequency information can be defined by the following expression:

[0015] wherein, represents high-pass filtering processing; The low-frequency information can be defined by the following expression:

[0016] wherein, represents low-pass filtering processing, represents spatial contrast, which can be defined by the following expression:

[0017] wherein, represents a mean image corresponding to the initial wide-field illumination image, This represents the mean image corresponding to the structured lighting image. Indicates Fourier transform, This represents the inverse Fourier transform.

[0018] In one embodiment, obtaining the wide-field optical slice tomography image corresponding to the initial wide-field illumination image based on the high-frequency information and the low-frequency information includes: The high-frequency and low-frequency information are weighted and summed to obtain the wide-field optical slice tomography image.

[0019] In one embodiment, the wide-field optical slice tomography image can be defined by the following expression:

[0020] in, The scaling factor represents the ratio of the high-frequency information to the low-frequency information.

[0021] Secondly, embodiments of the present invention provide a wide-field optical slice imaging device based on virtual illumination modulation, comprising: The acquisition module is used to acquire an initial wide-field illumination image and a modulation stripe image of the initial wide-field illumination image; The structured lighting image acquisition module is used to perform virtual lighting modulation based on the modulated stripe image and the initial wide-field lighting image to acquire the structured lighting image corresponding to the initial wide-field lighting image; The high-frequency and low-frequency information acquisition module is used to acquire high-frequency and low-frequency information corresponding to the initial wide-field illumination image based on the initial wide-field illumination image and the structure illumination image. The wide-field optical slice tomography image acquisition module is used to acquire the wide-field optical slice tomography image corresponding to the initial wide-field illumination image based on the high-frequency information and low-frequency information.

[0022] Thirdly, embodiments of the present invention provide an electronic device, including a memory and a processor, wherein the memory stores a computer program, and the processor executes the computer program to implement the steps of the wide-field optical slice imaging method based on virtual illumination modulation described in the first aspect.

[0023] The above technical solution has the following technical effects: Thus, the wide-field optical sectioning imaging method based on virtual illumination modulation provided in this embodiment acquires an initial wide-field illumination image and a modulation fringe image of the initial wide-field illumination image. Virtual illumination modulation is then performed based on the modulation fringe image and the initial wide-field illumination image to obtain a structural illumination image corresponding to the initial wide-field illumination image. This method is equivalent to applying modulation to the wide-field illumination, thereby replacing the actual illumination light field modulation process with virtual illumination modulation technology, thus avoiding the use of optical modulation devices. Furthermore, high-frequency and low-frequency information corresponding to the initial wide-field illumination image is obtained from the initial wide-field illumination image and the structural illumination image. Finally, a wide-field optical sectioning tomography image corresponding to the initial wide-field illumination image is obtained based on the weighted fusion of the high-frequency and low-frequency information. In this way, only one initial wide-field illumination image is needed to achieve the wide-field optical sectioning tomography effect. This not only improves the efficiency of wide-field optical sectioning imaging but also solves the problem of tissue scattering affecting traditional optical sectioning tomography techniques, thereby significantly improving the quality of wide-field optical sectioning imaging. Attached Figure Description

[0024] Figure 1 This is a flowchart illustrating a wide-field optical slice imaging method based on virtual illumination modulation, provided in an embodiment of the present invention. Figure 2 This is a schematic diagram of a wide-field fluorescence microscope for acquiring an initial wide-field illumination image according to an embodiment of the present invention; Figure 3 This is a schematic diagram of a wide-field optical slice imaging effect based on virtual illumination modulation provided in an embodiment of the present invention; Figure 4 A schematic diagram of a wide-field optical slice imaging device based on virtual illumination modulation provided in an embodiment of the present invention; Figure 5 This is a schematic diagram of the structure of an electronic device according to an embodiment of the present invention. Detailed Implementation

[0025] To further illustrate the various embodiments, the present invention provides accompanying drawings. These drawings are part of the disclosure of the present invention, primarily used to illustrate the embodiments and to explain the operating principles of the embodiments in conjunction with the relevant descriptions in the specification. With reference to these drawings, those skilled in the art should be able to understand other possible implementations and the advantages of the present invention. Components in the drawings are not drawn to scale, and similar component symbols are generally used to represent similar components.

[0026] The present invention will now be further described in conjunction with the accompanying drawings and specific embodiments.

[0027] Example 1: Figure 1This is a schematic flowchart of a wide-field optical slice imaging method based on virtual illumination modulation according to an embodiment of the present invention. This embodiment specifically includes the following steps: S10: Acquire the initial wide-field illumination image and the modulation stripe image of the initial wide-field illumination image.

[0028] in, Figure 2 This is a schematic diagram of a wide-field fluorescence microscope for acquiring an initial wide-field illumination image according to an embodiment of the present invention. Figure 3 This is a schematic diagram of a wide-field optical slice imaging effect based on virtual illumination modulation according to an embodiment of the present invention, with reference to... Figures 2-3 As shown, one method for acquiring the initial wide-field illumination image is as follows: a mercury lamp 1 forms Köhler illumination through lenses 2 and 3; further, the illumination is reflected by a dichroic mirror 4 and transmitted through an objective lens 5 to form wide-field illumination; then, the excited fluorescence signal is collected by the objective lens 5 and projected onto a camera 8 through a dichroic mirror filter 6 and a tube lens 7 to obtain the initial wide-field illumination image (a). The aforementioned modulated fringe image refers to a fringe image used for virtual illumination modulation of the initial wide-field illumination image, and the size of this modulated fringe image is the same as that of the initial wide-field illumination image. For example, continue to refer to... Figure 3 As shown, the modulated stripe image (b) corresponds to the initial wide-field illumination image (a), but it is not limited thereto. The present invention is not specifically limited, and those skilled in the art can set it according to the actual situation.

[0029] Specifically, an initial wide-field illumination image and a modulation stripe image are acquired for virtual illumination modulation processing of the initial wide-field illumination image.

[0030] Optionally, based on the above embodiments, in some embodiments of the present invention, the initial wide-field illumination image may be defined by the following expression:

[0031] in, x and y Represents the rectangular coordinates of the image plane. and Represents the rectangular coordinates of the object plane. Represents the distribution function. This represents the point spread function.

[0032] S11: Perform virtual illumination modulation based on the modulated stripe image and the initial wide-field illumination image to obtain the structured illumination image corresponding to the initial wide-field illumination image.

[0033] Specifically, after obtaining the initial wide-field illumination image and the modulation stripe image used for virtual illumination modulation of the initial wide-field illumination image, virtual illumination modulation is performed based on the modulation stripe image and the initial wide-field illumination image to obtain the structured illumination image corresponding to the initial wide-field illumination image.

[0034] Optionally, based on the above embodiments, in some embodiments of the present invention, one implementation of S11 may be: S111: Multiply the modulated stripe image and the initial wide-field illumination image to obtain the initial structure illumination image.

[0035] Specifically, the modulated fringe image and the initial wide-field illumination image are multiplied to obtain the initial structured illumination image.

[0036] Optionally, based on the above embodiments, in some embodiments of the present invention, the initial structure lighting image may be defined by the following expression:

[0037] in, This represents a modulated stripe image.

[0038] S112: Perform integration processing on the initial structured lighting image to obtain a structured lighting image.

[0039] Specifically, after obtaining the initial structured lighting image, the initial structured lighting image is integrated to obtain the structured lighting image.

[0040] For example, continue to refer to Figure 3 As shown, following the above embodiments, the initial structure lighting image is integrally processed to obtain the structure lighting image (c), but it is not limited thereto. The present invention is not specifically limited, and those skilled in the art can set it according to the actual situation.

[0041] Optionally, based on the above embodiments, in some embodiments of the present invention, the structured lighting image may be defined by the following expression:

[0042] , This indicates a convolution operation.

[0043] It should be noted that after multiplying the modulated stripe image and the initial wide-field illumination image and performing integration, the structured illumination image is obtained. This method is equivalent to applying modulation to the wide-field illumination, thereby realizing virtual illumination modulation technology to replace the real illumination light field modulation process, thus avoiding the use of optical modulation devices.

[0044] S12: Based on the initial wide-field illumination image and the structured illumination image, obtain the high-frequency information and low-frequency information corresponding to the initial wide-field illumination image.

[0045] Among them, high-frequency information includes high-frequency components of in-focus images, and low-frequency information includes low-frequency components of in-focus images and low-frequency components contained in out-of-focus information.

[0046] Specifically, after obtaining the structured illumination image, the high-frequency and low-frequency information corresponding to the initial wide-field illumination image are obtained based on the initial wide-field illumination image and the structured illumination image.

[0047] Optionally, based on the above embodiments, in some embodiments of the present invention, S12 may be implemented as follows: S121: Perform high-pass filtering on the initial wide-field illumination image to obtain high-frequency information.

[0048] Specifically, the initial wide-field illumination image is processed by a high-pass filter to obtain high-frequency information.

[0049] Optionally, based on the above embodiments, in some embodiments of the present invention, high-frequency information may be defined by the following expression:

[0050] in, This indicates high-pass filtering.

[0051] S122: Obtain spatial contrast based on the initial wide-field illumination image and the structured illumination image.

[0052] Spatial contrast can be defined by the following expression:

[0053] in, This represents the mean image corresponding to the initial wide-field illumination image. This represents the mean image corresponding to the structured lighting image. Indicates Fourier transform, This represents the inverse Fourier transform.

[0054] S123: Obtain low-frequency information based on spatial contrast and the initial wide-field illumination image.

[0055] Optionally, based on the above embodiments, in some embodiments of the present invention, one implementation of S123 may be: obtaining the product value of spatial contrast and initial wide-field illumination image; performing low-pass filtering on the product value to obtain low-frequency information.

[0056] Specifically, spatial contrast is obtained based on the initial wide-field illumination image and the structured illumination image. After obtaining the spatial contrast, the product of the spatial contrast and the initial wide-field illumination image is calculated. A low-pass filter is then used to perform low-pass filtering on the product of the spatial contrast and the initial wide-field illumination image to obtain low-frequency information.

[0057] Optionally, based on the above embodiments, in some embodiments of the present invention, the low-frequency information may be defined by the following expression:

[0058] in, This indicates low-pass filtering. Spatial contrast refers to the weighting function of in-focus information.

[0059] S13: Based on high-frequency and low-frequency information, obtain the wide-field optical slice tomography image corresponding to the initial wide-field illumination image.

[0060] Optionally, based on the above embodiments, in some embodiments of the present invention, one implementation of S13 may be: S131: Weighted summation of high-frequency and low-frequency information to obtain wide-field optical slice tomography images.

[0061] Specifically, for high-frequency and low-frequency information, a wide-field optical slice tomography image corresponding to the initial wide-field illumination image is obtained by weighted summation of the high-frequency and low-frequency information.

[0062] Optionally, based on the above embodiments, in some embodiments of the present invention, the wide-field optical slice tomography image may be defined by the following expression:

[0063] in, The scaling factor represents the ratio of high-frequency and low-frequency information. It should be noted that the scaling factor can ensure the seamless integration of high-frequency and low-frequency information, thereby improving the quality of wide-field optical slice imaging.

[0064] For example, continue to refer to Figure 3 As shown, following the above embodiments, the high-frequency information and low-frequency information are weighted and summed to obtain the wide-field optical slice tomography image (d) corresponding to the initial wide-field illumination image. However, this invention is not limited thereto and is not specifically limited. Those skilled in the art can set it according to the actual situation.

[0065] Thus, the wide-field optical sectioning imaging method based on virtual illumination modulation provided in this embodiment acquires an initial wide-field illumination image and a modulation fringe image of the initial wide-field illumination image. Virtual illumination modulation is then performed based on the modulation fringe image and the initial wide-field illumination image to obtain a structural illumination image corresponding to the initial wide-field illumination image. This method is equivalent to applying modulation to the wide-field illumination, thereby replacing the actual illumination light field modulation process with virtual illumination modulation technology, thus avoiding the use of optical modulation devices. Furthermore, high-frequency and low-frequency information corresponding to the initial wide-field illumination image is obtained from the initial wide-field illumination image and the structural illumination image. Finally, based on the weighted fusion of the high-frequency and low-frequency information, a wide-field optical sectioning tomography image corresponding to the initial wide-field illumination image is obtained. In this way, only one initial wide-field illumination image is needed to achieve the wide-field optical sectioning tomography effect. This not only improves the efficiency of wide-field optical sectioning imaging but also solves the problem of tissue scattering affecting traditional optical sectioning tomography techniques, thereby significantly improving the quality of wide-field optical sectioning imaging.

[0066] It should be understood that, although Figure 1 The steps in the flowchart are shown sequentially as indicated by the arrows, but these steps are not necessarily executed in the order indicated by the arrows. Unless otherwise explicitly stated herein, there is no strict order in which these steps are executed, and they can be performed in other orders. Furthermore, Figure 1 At least some of the steps in the process may include multiple sub-steps or multiple stages. These sub-steps or stages are not necessarily completed at the same time, but can be executed at different times. The execution order of these sub-steps or stages is not necessarily sequential, but can be executed in turn or alternately with other steps or at least some of the sub-steps or stages of other steps.

[0067] Example 2: The present invention also provides a wide-field optical slice imaging device based on virtual illumination modulation, such as... Figure 4 As shown, it includes: an acquisition module 10, a structured illumination image acquisition module 11, a high-frequency information and low-frequency information acquisition module 12, and a wide-field optical slice tomography image acquisition module 13.

[0068] The acquisition module 10 is used to acquire the initial wide-field illumination image and the modulation stripe image of the initial wide-field illumination image.

[0069] The structured lighting image acquisition module 11 is used to perform virtual lighting modulation based on the modulated stripe image and the initial wide-field lighting image to acquire the structured lighting image corresponding to the initial wide-field lighting image.

[0070] The high-frequency and low-frequency information acquisition module 12 is used to acquire high-frequency and low-frequency information corresponding to the initial wide-field illumination image based on the initial wide-field illumination image and the structure illumination image.

[0071] The wide-field optical slice tomography image acquisition module 13 is used to acquire the wide-field optical slice tomography image corresponding to the initial wide-field illumination image based on high-frequency information and low-frequency information.

[0072] In the above embodiments, an initial wide-field illumination image and a modulation fringe image of the initial wide-field illumination image are acquired by an acquisition module. A structure illumination image acquisition module performs virtual illumination modulation based on the modulation fringe image and the initial wide-field illumination image to acquire a structure illumination image corresponding to the initial wide-field illumination image. This method is equivalent to applying modulation to the wide-field illumination, thereby achieving virtual illumination modulation technology to replace the actual illumination light field modulation process, thus avoiding the use of optical modulation devices. Furthermore, a high-frequency information and low-frequency information acquisition module acquires the high-frequency and low-frequency information corresponding to the initial wide-field illumination image based on the initial wide-field illumination image and the structure illumination image. A wide-field optical section tomography image acquisition module acquires a wide-field optical section tomography image corresponding to the initial wide-field illumination image based on a weighted fusion of the high-frequency and low-frequency information. In this way, only one initial wide-field illumination image is needed to achieve the wide-field optical section tomography effect. This not only improves the efficiency of wide-field optical section imaging but also solves the problem of tissue scattering affecting traditional optical section tomography techniques, thereby significantly improving the quality of wide-field optical section imaging.

[0073] Example 3: The present invention also provides an electronic device, such as... Figure 5 As shown, Figure 5 This is a schematic diagram of the structure of an electronic device according to an embodiment of the present invention. The electronic device includes a processor 1101, a memory 1102, a bus 1103, and a computer program stored in the memory 1102 and executable on the processor 1101. The processor 1101 includes one or more processing cores. The memory 1102 is connected to the processor 1101 via the bus 1103. The memory 1102 is used to store program instructions. When the processor executes the computer program, it implements the steps in the above-described method embodiment of Embodiment 1 of the present invention.

[0074] Furthermore, as an executable solution, the electronic device can be a computer unit, which can be a desktop computer, laptop, handheld computer, cloud server, or other computing device. The computer unit may include, but is not limited to, a processor and memory. Those skilled in the art will understand that the above-described structure of the computer unit is merely an example and does not constitute a limitation on the computer unit. It may include more or fewer components, or combine certain components, or use different components. For example, the computer unit may also include input / output devices, network access devices, buses, etc., and this embodiment of the invention does not limit this.

[0075] Furthermore, as an executable solution, the processor can be a Central Processing Unit (CPU), or other general-purpose processors, digital signal processors (DSPs), application-specific integrated circuits (ASICs), field-programmable gate arrays (FPGAs), or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc. The general-purpose processor can be a microprocessor or any conventional processor, etc. The processor is the control center of the computer unit, connecting various parts of the entire computer unit via various interfaces and lines.

[0076] The memory can be used to store the computer programs and / or modules. The processor implements various functions of the computer unit by running or executing the computer programs and / or modules stored in the memory and by calling data stored in the memory. The memory may mainly include a program storage area and a data storage area. The program storage area may store the operating system and at least one application program required for a function; the data storage area may store data created based on the use of the mobile phone, etc. In addition, the memory may include high-speed random access memory and may also include non-volatile memory, such as hard disk, RAM, plug-in hard disk, smart media card (SMC), secure digital card (SD card), flash card, at least one disk storage device, flash memory device, or other volatile solid-state storage device.

[0077] The technical features of the above embodiments can be combined in any way. For the sake of brevity, not all possible combinations of the technical features in the above embodiments are described. However, as long as there is no contradiction in the combination of these technical features, they should be considered to be within the scope of this specification.

[0078] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are relatively specific and detailed, they should not be construed as limiting the scope of the invention patent. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these all fall within the protection scope of the present invention. Therefore, the protection scope of this invention patent should be determined by the appended claims.

Claims

1. A wide-field optical slice imaging method based on virtual illumination modulation, characterized in that, The method includes: Acquire an initial wide-field illumination image and a modulated stripe image of the initial wide-field illumination image; Virtual illumination modulation is performed based on the modulated stripe image and the initial wide-field illumination image to obtain a structured illumination image corresponding to the initial wide-field illumination image; Based on the initial wide-field illumination image and the structured illumination image, obtain the high-frequency information and low-frequency information corresponding to the initial wide-field illumination image; Based on the high-frequency and low-frequency information, a wide-field optical slice tomography image corresponding to the initial wide-field illumination image is obtained.

2. The method according to claim 1, characterized in that, The step of performing virtual illumination modulation based on the modulated stripe image and the initial wide-field illumination image to obtain the structured illumination image corresponding to the initial wide-field illumination image includes: The modulated stripe image and the initial wide-field illumination image are multiplied to obtain the initial structure illumination image; The initial structured illumination image is integrated to obtain the structured illumination image.

3. The method according to claim 2, characterized in that, The initial wide-field illumination image can be defined by the following expression: in, x and y Represents the rectangular coordinates of the image plane. and Represents the rectangular coordinates of the object plane. Represents the distribution function. Represents the point spread function; The initial structure illumination image can be defined by the following expression: in, Represents a modulated stripe image; The structured illumination image can be defined by the following expression: 。 4. The method according to claim 1, characterized in that, The step of obtaining high-frequency and low-frequency information corresponding to the initial wide-field illumination image based on the initial wide-field illumination image and the structured illumination image includes: The initial wide-field illumination image is subjected to high-pass filtering to obtain the high-frequency information; Spatial contrast is obtained based on the initial wide-field illumination image and the structured illumination image; The low-frequency information is obtained based on the spatial contrast and the initial wide-field illumination image.

5. The method according to claim 4, characterized in that, The step of obtaining the low-frequency information based on the spatial contrast and the initial wide-field illumination image includes: Obtain the product of the spatial contrast and the initial wide-field illumination image; The product value is subjected to low-pass filtering to obtain the low-frequency information.

6. The method according to claim 5, characterized in that, The high-frequency information can be defined by the following expression: in, This indicates high-pass filtering processing; The low-frequency information can be defined by the following expression: in, This indicates low-pass filtering. Spatial contrast can be defined by the following expression: in, This represents the mean image corresponding to the initial wide-field illumination image. This represents the mean image corresponding to the structured lighting image. Indicates Fourier transform, This represents the inverse Fourier transform.

7. The method according to claim 1, characterized in that, The step of obtaining a wide-field optical slice tomography image corresponding to the initial wide-field illumination image based on the high-frequency and low-frequency information includes: The high-frequency and low-frequency information are weighted and summed to obtain the wide-field optical slice tomography image.

8. The method according to claim 1, characterized in that, The wide-field optical slice tomography image can be defined by the following expression: in, The scaling factor represents the ratio of the high-frequency information to the low-frequency information.

9. A wide-field optical slice imaging device based on virtual illumination modulation, characterized in that, include: The acquisition module is used to acquire an initial wide-field illumination image and a modulation stripe image of the initial wide-field illumination image; The structured lighting image acquisition module is used to perform virtual lighting modulation based on the modulated stripe image and the initial wide-field lighting image to acquire the structured lighting image corresponding to the initial wide-field lighting image; The high-frequency and low-frequency information acquisition module is used to acquire high-frequency and low-frequency information corresponding to the initial wide-field illumination image based on the initial wide-field illumination image and the structure illumination image. The wide-field optical slice tomography image acquisition module is used to acquire the wide-field optical slice tomography image corresponding to the initial wide-field illumination image based on the high-frequency information and low-frequency information.

10. An electronic device comprising a memory and a processor, wherein the memory stores a computer program, characterized in that, When the processor executes the computer program, it implements the steps of the wide-field optical slice imaging method based on virtual illumination modulation as described in any one of claims 1 to 8.

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