Synergistic composition containing isoxazoline insecticidal and acaricide and application thereof

Abstract

The invention discloses a synergistic composition containing isoxazoline insecticidal and acaricide, which is composed of a compound I and a compound II, the compound I is CCXN-15-005-S (S configuration) or an agrochemically acceptable salt thereof, and the compound II is CCXN-15-005-S (S configuration) or an agrochemically acceptable salt thereof. The compound II is selected from any one of a nicotinic acetylcholine receptor agonist, a chordinal TRPV channel regulator, an acetyl-coenzyme A carboxylase (ACCase) inhibitor, a mitochondrial electron transfer inhibitor, a calcium-activated potassium ion channel regulator and trifluoroethyl thioether (sulfoxide) or an agrochemically acceptable salt thereof. The synergistic composition can be used for preventing and treating pests and harmful mites of thysanoptera, hemiptera and acarus and has a wider agricultural application prospect.

Description

Technical Field

[0001] This invention relates to a synergistic composition containing isoxazoline insecticides and acaricides and its application. It belongs to the field of pesticide compounding technology. Background Technology

[0002] Pesticides are an irreplaceable strategic resource for ensuring food security and a crucial weapon in controlling pests, diseases, and weeds. Currently, my country faces severe challenges in the green control of major agricultural pests, with over 50% of traditional varieties exhibiting medium to high risk of resistance. Most mainstream insecticides and acaricides on the market have been used for decades, resulting in significant resistance issues and a gradual decline in their control effectiveness. In the absence of highly effective insecticides and acaricides on the market, farmers, in an attempt to improve control, resort to blindly increasing dosages or arbitrarily mixing pesticides. This unscientific and irrational use not only fails to achieve synergistic effects but also leads to pesticide waste, excessive residues, environmental pollution, and further exacerbates resistance.

[0003] Therefore, there is an urgent need to develop synergistic combinations of insecticides and acaricides that can achieve synergistic effects, improve control efficacy, reduce application dosage, decrease the number of applications, and lower usage costs; expand the range of pests to be controlled, compensate for the deficiencies of existing market products, and delay the development of resistance. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of the prior art. This invention provides a synergistic composition containing isoxazoline insecticides and acaricides and its application.

[0005] To achieve the above objectives, the present invention adopts the following technical solution:

[0006] 1. A synergistic composition containing an isoxazoline insecticide and acaricide, comprising compound I and compound II, wherein compound I is CCXN-15-005-S or an agriculturally acceptable salt thereof, and compound II is selected from any one of the following compounds or an agriculturally acceptable salt thereof: nicotinic acetylcholine receptor agonists, string organ TRPV channel modulators, acetyl-CoA carboxylase (ACCase) inhibitors, mitochondrial electron transport inhibitors, calcium-activated potassium ion channel modulators, and trifluoroethyl sulfides (sulfoxides); wherein the chemical name of CCXN-15-005-S is (S)-4-(5-(3,5-dichloro-4-fluorophenyl)-5-(trifluoromethyl)-4,5-dihydroisoxazo-3-yl)-N-((methoxyimino)methyl)-2-methylbenzamide, and its chemical structural formula is as follows:

[0007]

[0008] Preferably, the mass ratio of compound I to compound II is 1:50 to 50:1.

[0009] Preferably, the nicotinic acetylcholine receptor agonist is selected from any one of acetamiprid, imidacloprid, thiamethoxam, acetamiprid, dinotefuran, thiamethoxam, thiamethoxam, chlorothiazoline, epoxiconazole, piperazine, chlorpyrifos, nicotine, flonicamid, and flupyrflufenoxam.

[0010] Preferably, the TRPV channel modulator of the string instrument is selected from any one of flufenoxuron, flonicamid, and pyraclostrobin.

[0011] Preferably, the acetyl-CoA carboxylase inhibitor is selected from spirotetramat or methoxypiperidine ethyl ester.

[0012] Preferably, the mitochondrial electron transport inhibitor is selected from any one of flufenoxuron, pyridaben, pyrazoyl aniline, etoxazole, fenproxone, bifenazate, pyrimethanil, pyridaben, pyridaben, quinacrine, pyrimethanil, pyridaben, pyridaben, and fenproxamide.

[0013] Preferably, the calcium-activated potassium ion channel modulator is flufenoxuron.

[0014] Preferably, the trifluoroethyl sulfide (sulfoxide) is flupentixol.

[0015] 2. Application of the aforementioned composition in the preparation of agricultural insecticides and acaricides.

[0016] Preferably, the targets of the agricultural insecticide and acaricide are: Hemiptera, Thysanoptera, Hemiptera, and Acari.

[0017] More preferably, the targets of the agricultural insecticide and acaricide are: Aphididae (Hemiptera), Thripsidae (Thysanoptera), Whiteflyidae (Hemiptera), and Tetranychidae (Acari).

[0018] Preferably, compound II in the composition is a nicotinic acetylcholine receptor agonist, and the corresponding agricultural insecticide and acaricide targets Hemiptera and Hemiptera.

[0019] More preferably, compound II in the composition is flupyrfuranone, and the target pests of the corresponding agricultural insecticide and acaricide are aphids and whiteflies.

[0020] More preferably, compound II in the composition is imidacloprid, and the target pests of the corresponding agricultural insecticide and acaricide are aphids and whiteflies.

[0021] Preferably, compound II in the composition is a string instrument TRPV channel modulator, and the target pests of the corresponding agricultural insecticides and acaricides are: Homoptera, Thysanoptera, Hemiptera, and Acari.

[0022] More preferably, compound II in the composition is flufenoxuron, and the target pests of the corresponding agricultural insecticide and acaricide are: aphids, whiteflies, thrips, and two-spotted spider mites.

[0023] More preferably, compound II in the composition is flonicamid, and the target pests of the corresponding agricultural insecticide and acaricide are aphids and whiteflies.

[0024] More preferably, compound II in the composition is azirconazole, and the target pests of the corresponding agricultural insecticide and acaricide are aphids and whiteflies.

[0025] Preferably, compound II in the composition is an acetyl-CoA carboxylase inhibitor, and the target pests of the corresponding agricultural insecticides and acaricides are: Homoptera, Hemiptera, and Acari.

[0026] More preferably, compound II in the composition is methoxypiperidine ethyl ester, and the target pests of the corresponding agricultural insecticide and acaricide are: aphids, whiteflies, and two-spotted spider mites.

[0027] Preferably, compound II in the composition is a mitochondrial electron transport inhibitor, and the target pests of the corresponding agricultural insecticides and acaricides are: Homoptera, Thysanoptera, Hemiptera, and Acari.

[0028] More preferably, compound II in the composition is flufenoxuron-methyl, and the target pests of the corresponding agricultural insecticide and acaricide are: aphids, whiteflies, thrips, and two-spotted spider mites.

[0029] More preferably, compound II in the composition is pyridaben, and the target pests of the corresponding agricultural insecticide and acaricide are: two-spotted spider mite, kasugamys spider mite, and citrus psyllid.

[0030] More preferably, compound II in the composition is pyrazolyl aniline, and the target pests of the corresponding agricultural insecticide and acaricide are: two-spotted spider mite and citrus psyllid.

[0031] More preferably, compound II in the composition is etoxazole, and the target pest of the corresponding agricultural insecticide and acaricide is two-spotted spider mite.

[0032] More preferably, compound II in the composition is acaricide quinone, and the target pests of the corresponding agricultural insecticide and acaricide are: two-spotted spider mite, Kanzawa spider mite, and citrus psyllid.

[0033] Preferably, compound II in the composition is a calcium-activated potassium ion channel modulator, and the target pests of the corresponding agricultural insecticides and acaricides are mites.

[0034] More preferably, compound II in the composition is flufenoxuron, and the target pests of the corresponding agricultural insecticide and acaricide are: two-spotted spider mite and citrus pterocaryon.

[0035] Preferably, compound II in the composition is a trifluoroethyl sulfide (sulfoxide) class agricultural insecticide and acaricide targeting mites.

[0036] More preferably, compound II in the composition is flufenoxuron, and the target pests of the corresponding agricultural insecticide and acaricide are: two-spotted spider mite, Kanzawa spider mite, and citrus psyllid.

[0037] 3. An agricultural insecticide and acaricide comprising the aforementioned composition.

[0038] Preferably, the agricultural insecticide and acaricide further comprises an agriculturally chemically acceptable excipient.

[0039] Preferably, the formulations of the agricultural insecticides and acaricides include, but are not limited to: water dispersants, dispersible liquids, wettable powders, soluble powders, suspensions, water emulsions, microemulsions, suspension emulsions, microcapsule suspensions, emulsifiable concentrates, and granules.

[0040] The beneficial effects of this invention are:

[0041] This invention discloses a synergistic composition containing isoxazoline insecticides and acaricides, comprising compound I and compound II. Compound I is CCXN-15-005-S (S configuration) or an agriculturally acceptable salt thereof. Compound II is selected from any one of the following compounds: nicotinic acetylcholine receptor agonists, string organ TRPV channel modulators, acetyl-CoA carboxylase (ACCase) inhibitors, mitochondrial electron transport inhibitors, calcium-activated potassium ion channel modulators, and trifluoroethyl sulfides (sulfoxides), or an agriculturally acceptable salt thereof. This synergistic composition can be used for the control of pests and mites in the orders Homoptera, Thysanoptera, Hemiptera, and Acari, and has broad agricultural application prospects.

[0042] This invention has the following advantages:

[0043] 1) When used in combination with nicotinic acetylcholine receptor agonists, string organ TRPV channel modulators, acetyl-CoA carboxylase (ACCase) inhibitors, mitochondrial electron transport inhibitors, calcium-activated potassium ion channel modulators, and trifluoroethyl sulfide (sulfoxide) agents, it has a synergistic effect and can significantly improve the prevention and control effect.

[0044] 2) When used in combination with tone-regulating insecticides, it effectively solves the problem of slow onset of action of tone-regulating insecticides. The combination has a fast onset of action and significantly improves the control effect.

[0045] 3) When used in combination with mitochondrial electron transport inhibitors and calcium-activated potassium ion channel regulators, it effectively solves the problem of poor activity of mitochondrial electron transport inhibitors and calcium-activated potassium ion channel regulators against the egg stage of mites. After mixing, it provides highly efficient control of mites throughout their entire life cycle.

[0046] 3) The two active ingredients have different mechanisms of action, and their combined use can delay the development of pesticide resistance in pests;

[0047] 4) Reduce the amount of pesticide applied, reduce the number of applications, and lower the cost of use. Attached Figure Description

[0048] Figure 1 The synthetic route for CCXN-15-005-S is shown below.

[0049] Figure 2 This is the proton spectrum of CCXN-15-005-S;

[0050] Figure 3 This is the synthetic route for the racemate of CCXN-15-005;

[0051] Figure 4 This is the proton NMR spectrum of the racemic mixture of CCXN-15-005. Detailed Implementation

[0052] The present invention will be further described below with reference to the accompanying drawings and embodiments. It should be noted that the following description is only for explaining the present invention and does not limit its content.

[0053] Reference Implementation Examples

[0054] Preparation of CCXN-15-005-S

[0055] See the synthetic route. Figure 1 Specifically, it includes the following steps:

[0056] (1) Preparation of intermediate 1:

[0057] Take 100g (0.23mol) of 4-(5-(3,5-dichloro-4-fluorophenyl)-5-(trifluoromethyl)-4,5-dihydroisoxazol-3-yl)-2-methylbenzoic acid (Dezhou Hanhua Pharmaceutical Chemical Co., Ltd.), dissolve it in a mixed solution of 171g n-butanol, 680g acetonitrile, and 45g water, and stir evenly at room temperature. Add 31g (0.23mol) of resolving agent (R)-1-(4-methylphenyl)ethylamine (Biode Pharmaceutical), 19g n-butanol, 76g acetonitrile, and 5g water. Heat to 60℃ and stir for 2h. Cool to 20℃ and stir for 3h. A large amount of solid precipitates out. Filter to obtain the solid. The solid was added to a mixed solution of 900g acetonitrile and 100g water, heated to 60℃, stirred until dissolved, cooled to 20℃, stirred for 3h, filtered, and dried at 50℃ for 20h to obtain 73g of the resolved salt. The 73g of the resolved salt was added to 730g of toluene, heated to 60℃, and 300g of 3M hydrochloric acid aqueous solution was added. The mixture was stirred for 30min to separate the aqueous phase. The organic phase was concentrated at 60℃ to obtain approximately 80% toluene by volume. 400g of n-heptane was added, the mixture was stirred, cooled to 20℃, stirred for 3h, filtered, washed with 40g of n-heptane, and dried at 50℃ for 20h to obtain intermediate 1, 43.41g, with a yield of 43.41% and a chiral purity greater than 90%.

[0058] (2) Preparation of intermediate 2:

[0059] Take 140 g (0.09 mol) of intermediate, add 174.4 g of toluene, 17.45 g of thionyl chloride, and 1 g of DMF, stir and heat, and react at 55 °C for 4 h. After the reaction is complete, concentrate to dryness under reduced pressure, add 174.4 g of toluene to dissolve, and obtain the prepared solution.

[0060] Take 25 g (0.37 mol) of ammonia water (25% aqueous solution) and 35 g of toluene, stir and cool. Add the prepared solution dropwise at 5 °C. After the addition is complete, react at 20 °C for 4 h. After the reaction is complete, filter, wash with 200 g of water, and dry in a forced-air environment at 50 °C for 20 h to obtain 35.8 g, with a molar yield of 89.7%.

[0061] (3) Preparation of CCXN-15-005-S:

[0062] Take 35.8 g (0.08 mol) of intermediate 2, add 218.5 g of toluene and 12.73 g (0.10 mol) of DMFDMA (N,N-dimethylformamide dimethyl acetal), stir and heat, and react at 75 °C for 4 h. After the reaction is complete, add 8.3 g (0.10 mol) of methoxyamine hydrochloride, and continue to react at 75 °C for 4 h. After the reaction is complete, cool to -20 °C to crystallize for 16 h, and filter to obtain the crude product.

[0063] The crude product was added to a mixed solution of 161g ethyl acetate and 72g water, stirred to dissolve, separated, dried over anhydrous sodium sulfate, concentrated to dryness at 45℃, 110g methanol was added, stirred at 20℃ for 3h, filtered, and dried under forced air at 50℃ for 24h to obtain 31.12g, with a molar yield of 76.83% and a chiral purity greater than 93%.

[0064] The proton NMR spectrum of product CCXN-15-005-S is shown below. Figure 2 .

[0065] 1 H NMR(400MHz, CDCl3)δ8.480-8.504(d,1H),7.752-7.774(d,1H),7.548–7.595(m,5H),4.07 0-4.113(d,1H),3.897(s,2.72H),3.797(s,0.37H),3.678-3.721(d,1H),2.526(s,3H)..C 20 H 15 Cl2F4N3O3, [M+H] + =492.0495, [MH] - =490.0339.

[0066] Example

[0067] A synergistic composition containing isoxazoline insecticides and acaricides is derived from compound I (obtained from the reference example).

[0068] The compound consists of compound I (CCXN-15-005-S) and compound II, with the mass ratio of compound I to compound II (Dezhou Hanhua Pharmaceutical Chemical Co., Ltd.) being 50:1, 40:1, 30:1, 20:1, 10:1, 1:1, 1:10, 1:20, 1:30, 1:40, and 1:50.

[0069] Comparative Example

[0070] The composition comprises compound III: CCXN-15-005 (racemic mixture) and compound II, with mass ratios of compound III to compound II of 50:1, 40:1, 30:1, 20:1, 10:1, 1:1, 1:10, 1:20, 1:30, 1:40, and 1:50. The preparation process of CCXN-15-005 (racemic mixture) is described below. Figure 3 Specifically, it includes the following steps:

[0071] (1) Preparation of intermediate 1:

[0072] Take 40 g (0.09 mol) of 4-(5-(3,5-dichloro-4-fluorophenyl)-5-(trifluoromethyl)-4,5-dihydroisoxazol-3-yl)-2-methylbenzoic acid, add 174.4 g of toluene, 17.45 g of thionyl chloride, and 1 g of DMF, stir and heat to 55 °C for 4 h. After the reaction is complete, concentrate to dryness under reduced pressure, add 174.4 g of toluene to dissolve, and obtain the prepared solution.

[0073] Take 25 g (0.37 mol) of ammonia water (25% aqueous solution) and 35 g of toluene, stir and cool. Add the prepared solution dropwise at 5 °C. After the addition is complete, react at 20 °C for 4 h. After the reaction is complete, filter, wash with 200 g of water, and dry in a forced-air environment at 50 °C for 20 h to obtain 37.1 g, with a molar yield of 93.0%.

[0074] (2) Preparation of the racemate of CCXN-15-005:

[0075] Take 35.8 g (0.08 mol) of intermediate 2, add 218.5 g of toluene and 12.73 g (0.10 mol) of DMFDMA (N,N-dimethylformamide dimethyl acetal), stir and heat, and react at 75 °C for 4 h. After the reaction is complete, add 8.3 g (0.10 mol) of methoxyamine hydrochloride, and continue to react at 75 °C for 4 h until the reaction is complete. Cool to 5 °C and crystallize for 16 h, then filter to obtain the crude product.

[0076] The crude product was added to a mixed solution of 161g ethyl acetate and 72g water, stirred to dissolve, separated, the organic phase was dried with anhydrous sodium sulfate, concentrated to dryness at 45℃, 110g methanol was added, stirred at 20℃ for 3h, filtered, and dried at 50℃ for 24h to obtain 34.41g, with a molar yield of 85.0%.

[0077] The proton NMR spectrum of the racemic product CCXN-15-005 is shown below. Figure 4 .

[0078] 1 H NMR(400MHz, CDCl3)δ8.483-8.507(d,1H),7.752-7.775(d,1H),7.528–7.594( m,5H),4.071-4.114(d,1H),3.897(s,3H),3.679-3.722(d,1H),2.527(s,3H).

[0079] C 20 H 15 Cl2F4N3O3, [M+H] + =492.0501, [MH] - =490.0343.

[0080] Test case

[0081] The insecticidal and acaricidal effects of the compositions of the examples, the comparative compositions, and compounds I (CCXN-15-005-S obtained with reference to the examples), II, and III (CCXN-15-005 racemate) were tested.

[0082] In accordance with the agricultural industry standards of the People's Republic of China, "Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides Part 6: Insecticide Activity Test - Insect Immersion Method" (NY / T 1154.6-2006) and "Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides Part 14: Leaf Immersion Method" (NY / T 1154.14-2008), this experiment uses the insect immersion and leaf immersion methods to determine the indoor toxicity of the test samples to aphids.

[0083] According to the People's Republic of China agricultural industry standard "Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides Part 8: Filter Paper Film Method" (NY / T 1154.8-2007), this test uses the filter paper film method to determine the indoor toxicity of the test samples to thrips.

[0084] According to the People's Republic of China agricultural industry standard "Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides Part 16: Activity Tests against Whiteflies - Agar Moisture Dipping Method" (NY / T 1154.16-2013), this test uses the agar moisture dipping method to determine the indoor toxicity of the test samples against whiteflies.

[0085] According to the agricultural industry standard of the People's Republic of China "Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides Part 12: Tetranychus Slide Impregnation Method" (NY / T 1154.12-2008), this experiment uses the Tetranychus slide impregnation method to determine the indoor toxicity of the test samples against Tetranychus two-spotted, Tetranychus citrus, and Tetranychus kanazei.

[0086] According to the agricultural industry standard of the People's Republic of China "Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides Part 5: Ovicidal Activity Test - Immersion Method" (NY / T 1154.5-2006), this test uses the immersion method to determine the indoor toxicity of the test samples to the eggs of Tetranychus simonii, Tetranychus citrus, and Tetranychus kanazei.

[0087] 1. Compound II is flupyrrolidone, and the results are shown in Table 1.

[0088] Table 1. Indoor bioassay results for Compound I: CCXN-15-005-S and Compound II: Flupyrofurone; Indoor bioassay results for Compound III: CCXN-15-005 (racemic) and Compound II: Flupyrofurone.

[0089]

[0090] From Table 1, we can draw the following conclusions:

[0091] For the target aphid, samples of compound I:CCXN-15-005-S and compound II: flupyrrolidone mixed in different mass ratios showed 2-3 times higher efficacy than compound I:CCXN-15-005-S alone; samples of compound I:CCXN-15-005-S and compound II: flupyrrolidone mixed in different mass ratios showed 4-6 times higher efficacy than compound II: flupyrrolidone alone.

[0092] For the target whitefly, samples containing a mixture of compound I:CCXN-15-005-S and compound II: flupyrrolidone at different mass ratios showed a 3-5 times higher efficacy compared to using compound I:CCXN-15-005-S alone; samples containing a mixture of compound I:CCXN-15-005-S and compound II: flupyrrolidone at different mass ratios showed a 68-93 times higher efficacy compared to using compound II: flupyrrolidone alone.

[0093] For the target aphid, samples of mixtures of compound III: CCXN-15-005 (racemic) and compound II: flupyrrolidone at different mass ratios showed comparable efficacy to compound III: CCXN-15-005 (racemic) alone;

[0094] Samples containing mixtures of compound III:CCXN-15-005 (racemic) and compound II:flupyrrolidone at different mass ratios showed approximately 90% lower efficacy compared to compound II:flupyrrolidone alone.

[0095] The target whitefly, samples of compounds III: CCXN-15-005 (racemic) and compound II: flupyrrolidone mixed in different mass ratios showed comparable efficacy to compound III: CCXN-15-005 (racemic) alone;

[0096] Samples containing mixtures of compound III:CCXN-15-005 (racemic) and compound II:flupyrfuranone at different mass ratios showed approximately 15% lower efficacy compared to compound II:flupyrfuranone alone.

[0097] Therefore, compared with the use of compound I:CCXN-15-005-S, compound II: flupyrfuranone, or compound alone...

[0098] Compared to III:CCXN-15-005 (racemic mixture), the combination of compound I:CCXN-15-005-S and compound II:flupyrrolidone has a significantly better control effect on aphids and whiteflies.

[0099] 2. Compound II is imidacloprid, and the results are shown in Table 2.

[0100] Table 2. Results of in vitro bioassays for Compound I: CCXN-15-005-S and Compound II: Imidacloprid

[0101]

[0102] From Table 2, we can draw the following conclusions:

[0103] For the target aphid, samples of compounds I:CCXN-15-005-S and compound II: imidacloprid mixed in different mass ratios showed 2-3 times higher efficacy than compound I:CCXN-15-005-S alone; samples of compounds I:CCXN-15-005-S and compound II: imidacloprid mixed in different mass ratios showed 28-32 times higher efficacy than compound II: imidacloprid alone.

[0104] For the target whitefly, samples containing a mixture of compound I:CCXN-15-005-S and compound II: imidacloprid at different mass ratios showed 2-3 times higher efficacy than compound I:CCXN-15-005-S alone; samples containing a mixture of compound I:CCXN-15-005-S and compound II: imidacloprid at different mass ratios showed 131-176 times higher efficacy than compound II: imidacloprid alone.

[0105] Therefore, compared with using compound I:CCXN-15-005-S and compound II: imidacloprid alone, compound

[0106] The combination of compound I:CCXN-15-005-S and compound II:imidacloprid has a significantly better control effect on aphids and whiteflies.

[0107] 3. Compound II is flufenoxuron, and the results are shown in Table 3.

[0108] Table 3. Indoor biochemical test results of compound I: CCXN-15-005-S and compound II: fipronil; indoor biochemical test results of compound III: CCXN-15-005 (racemic) and compound II: fipronil.

[0109]

[0110] From Table 3, we can draw the following conclusions:

[0111] Target aphids, observed for 48 hours. Samples containing a mixture of compound I: CCXN-15-005-S and compound II: flufenoxuron at different mass ratios showed 1.5-2.5 times higher efficacy compared to compound I: CCXN-15-005-S alone.

[0112] Samples containing compound I:CCXN-15-005-S and compound II:flufenican at different mass ratios showed 3-5 times greater efficacy than compound II:flufenican alone.

[0113] Target aphids, observed for 72 hours. Samples containing a mixture of compound I: CCXN-15-005-S and compound II: flufenoxuron at different mass ratios showed 1.5-2.5 times higher efficacy compared to compound I: CCXN-15-005-S alone.

[0114] Samples containing compound I:CCXN-15-005-S and compound II:flufenican at different mass ratios showed 2-3 times greater efficacy than compound II:flufenican alone.

[0115] Target aphids, observed for 96 hours. Samples containing a mixture of compound I:CCXN-15-005-S and compound II: flufenoxuron at different mass ratios showed 2-3 times higher efficacy compared to compound I:CCXN-15-005-S alone.

[0116] Samples containing compound I:CCXN-15-005-S and compound II:flufenican at different mass ratios showed 2-3 times greater efficacy than compound II:flufenican alone.

[0117] For the target whitefly, samples containing a mixture of compound I:CCXN-15-005-S and compound II: flufenoxuron at different mass ratios showed a 4-5 times higher efficacy compared to using compound I:CCXN-15-005-S alone; samples containing a mixture of compound I:CCXN-15-005-S and compound II: flufenoxuron at different mass ratios showed a 24-31 times higher efficacy compared to using compound II: flufenoxuron alone.

[0118] For the target thrips, samples of compound I:CCXN-15-005-S and compound II: flufenoxuron mixed in different mass ratios showed 2-3 times higher efficacy than compound I:CCXN-15-005-S alone; samples of compound I:CCXN-15-005-S and compound II: flufenoxuron mixed in different mass ratios showed 34-59 times higher efficacy than compound II: flufenoxuron alone.

[0119] The target spider mite, two-spotted spider mite, showed that samples of compound I:CCXN-15-005-S and compound II: flufenoxuron mixed in different mass ratios were 3-5 times more effective than compound I:CCXN-15-005-S alone; samples of compound I:CCXN-15-005-S and compound II: flufenoxuron mixed in different mass ratios were 290-467 times more effective than compound II: flufenoxuron alone.

[0120] Target aphids, observed for 48 hours. Samples containing mixtures of compound III:CCXN-15-005 (racemic) and compound II: flufenoxuron at different mass ratios showed comparable efficacy to compound III:CCXN-15-005 (racemic) alone. Samples containing mixtures of compound III:CCXN-15-005 (racemic) and compound II: flufenoxuron at different mass ratios showed approximately 70% lower efficacy compared to compound II: flufenoxuron alone.

[0121] Target aphids, observed for 72 hours. Samples containing mixtures of compound III:CCXN-15-005 (racemic) and compound II: flufenoxuron at different mass ratios showed comparable efficacy to compound III:CCXN-15-005 (racemic) alone. Samples containing mixtures of compound III:CCXN-15-005 (racemic) and compound II: flufenoxuron at different mass ratios showed approximately 80% lower efficacy compared to compound II: flufenoxuron alone.

[0122] Target aphids, observed for 96 hours. Samples containing mixtures of compound III:CCXN-15-005 (racemic) and compound II: flufenoxuron at different mass ratios showed comparable efficacy to compound III:CCXN-15-005 (racemic) alone. Samples containing mixtures of compound III:CCXN-15-005 (racemic) and compound II: flufenoxuron at different mass ratios showed approximately 80% lower efficacy compared to compound II: flufenoxuron alone.

[0123] The target whitefly, samples of compounds III: CCXN-15-005 (racemic) and compound II: flufenoxuron at different mass ratios showed comparable efficacy to compound III: CCXN-15-005 (racemic) alone;

[0124] Samples containing mixtures of compound III:CCXN-15-005 (racemic) and compound II:flufenican at different mass ratios showed approximately 40% lower efficacy compared to compound II:flufenican used alone.

[0125] The efficacy of a mixture of compound III:CCXN-15-005 (racemic) and compound II: flufenoxuron in different mass ratios against thrips was reduced by approximately 30% compared to the efficacy of compound III:CCXN-15-005 (racemic) alone.

[0126] Samples containing compound III:CCXN-15-005 (racemic mixture) and compound II:flufenican at different mass ratios showed approximately twice the efficacy of compound II:flufenican alone.

[0127] The efficacy of a mixture of compound III:CCXN-15-005 (racemic) and compound II: flufenoxuron in different mass ratios against the target spider mite was reduced by about 30% compared to the use of compound III:CCXN-15-005 (racemic) alone; the efficacy of a mixture of compound III:CCXN-15-005 (racemic) and compound II: flufenoxuron in different mass ratios was increased by about 2 times compared to the use of compound II: flufenoxuron alone.

[0128] Therefore, compared with the use of compound I:CCXN-15-005-S, compound II: flufenoxuron, or compound alone...

[0129] Compared to III:CCXN-15-005 (racemic mixture), the combination of compound I:CCXN-15-005-S and compound II:flufenican has significantly better control effects against aphids, whiteflies, thrips, and two-spotted spider mites.

[0130] 4. Compound II is flonicamid, and the results are shown in Table 4.

[0131] Table 4. Results of in vitro bioassays for Compound I: CCXN-15-005-S and Compound II: Flupyradifurone

[0132]

[0133] From Table 4, we can draw the following conclusions:

[0134] Target aphids, observed for 48 hours. Samples containing a mixture of compound I: CCXN-15-005-S and compound II: flonicamid at different mass ratios showed 2-3 times higher efficacy compared to compound I: CCXN-15-005-S alone.

[0135] Samples containing compound I:CCXN-15-005-S and compound II:fluoxetine at different mass ratios showed more than 700 times greater efficacy than compound II:fluoxetine alone.

[0136] Target aphids, observed for 72 hours. Samples containing a mixture of compound I: CCXN-15-005-S and compound II: flonicamid at different mass ratios showed 2-3 times higher efficacy compared to compound I: CCXN-15-005-S alone.

[0137] Samples containing compound I:CCXN-15-005-S and compound II:fluoxetine at different mass ratios showed 6-10 times greater efficacy than compound II:fluoxetine alone.

[0138] For the target whitefly, samples containing compounds I:CCXN-15-005-S and II: flonicamid at different mass ratios showed 6-10 times higher efficacy than compound I:CCXN-15-005-S alone; samples containing compounds I:CCXN-15-005-S and II: flonicamid at different mass ratios showed 625-915 times higher efficacy than compound II: flonicamid alone.

[0139] Therefore, compared with using compound I:CCXN-15-005-S and compound II: flonicamid alone, compound

[0140] The combination of compound I:CCXN-15-005-S and compound II:fluoxetine has a significantly better control effect on aphids and whiteflies.

[0141] 5. Compound II is azithromycinamide, and the results are shown in Table 5.

[0142] Table 5: Indoor bioassay results for Compound I: CCXN-15-005-S and Compound II: Imamectin

[0143]

[0144] From Table 5, we can draw the following conclusions:

[0145] Target aphids, observed for 48 hours. Samples containing a mixture of compound I: CCXN-15-005-S and compound II: chlorfenapyr at different mass ratios showed 2-3 times higher efficacy compared to compound I: CCXN-15-005-S alone.

[0146] Samples containing compound I:CCXN-15-005-S and compound II: chlorfenapyr at different mass ratios showed 234-482 times greater efficacy than compound II: chlorfenapyr alone.

[0147] Target aphids, observed for 72 hours. Samples containing a mixture of compound I: CCXN-15-005-S and compound II: chlorfenapyr at different mass ratios showed 2-4 times higher efficacy compared to compound I: CCXN-15-005-S alone.

[0148] Samples containing compound I:CCXN-15-005-S and compound II: chlorfenapyramide at different mass ratios showed 25-50 times greater efficacy than compound II: chlorfenapyramide alone.

[0149] Target aphids, observed for 96 hours. Samples containing a mixture of compound I: CCXN-15-005-S and compound II: chlorfenapyr at different mass ratios showed 2-4 times higher efficacy compared to compound I: CCXN-15-005-S alone.

[0150] Samples containing compound I:CCXN-15-005-S and compound II: chlorfenapyr at different mass ratios showed 11-23 times greater efficacy than compound II: chlorfenapyr alone.

[0151] For the target whitefly, samples containing a mixture of compound I:CCXN-15-005-S and compound II: chlorfenapyr at different mass ratios showed 2-3 times higher efficacy than compound I:CCXN-15-005-S alone; samples containing a mixture of compound I:CCXN-15-005-S and compound II: chlorfenapyr at different mass ratios showed 9-14 times higher efficacy than compound II: chlorfenapyr alone.

[0152] Therefore, compared with using compound I:CCXN-15-005-S and compound II: fenpropathrin alone, compound...

[0153] The combination of compound I:CCXN-15-005-S and compound II: chlorfenapyr has a significantly better control effect on aphids and whiteflies.

[0154] 6. Compound II is methoxypiperidine ethyl ester, and the results are shown in Table 6.

[0155] Table 6. Indoor bioassay results for Compound I: CCXN-15-005-S and Compound II: Ethyl methoxypiperidine; Indoor bioassay results for Compound III: CCXN-15-005 (racemic) and Compound II: Ethyl methoxypiperidine.

[0156]

[0157]

[0158] From Table 6, we can draw the following conclusions:

[0159] Target aphids, observed for 48 hours, samples containing a mixture of compound I: CCXN-15-005-S and compound II: methoxypiperidine ethyl ester at different mass ratios showed 2-3 times higher efficacy compared to compound I: CCXN-15-005-S alone;

[0160] Samples containing compound I:CCXN-15-005-S and compound II: methoxypiperidine ethyl ester at different mass ratios showed 47-76 times greater efficacy than compound II: methoxypiperidine ethyl ester alone.

[0161] Target aphids, observed for 72 hours, showed that samples mixed with compound I: CCXN-15-005-S and compound II: methoxypiperidine ethyl ester at different mass ratios exhibited 3-4 times higher efficacy compared to compound I: CCXN-15-005-S alone;

[0162] Samples containing compound I:CCXN-15-005-S and compound II: methoxypiperidine ethyl ester at different mass ratios showed 26-40 times greater efficacy than compound II: methoxypiperidine ethyl ester alone.

[0163] Target aphids, observed for 96 hours, samples containing a mixture of compound I: CCXN-15-005-S and compound II: methoxypiperidine ethyl ester at different mass ratios showed 2-4 times higher efficacy compared to compound I: CCXN-15-005-S alone;

[0164] Samples containing compound I:CCXN-15-005-S and compound II: methoxypiperidine ethyl ester at different mass ratios showed 10-21 times greater efficacy than compound II: methoxypiperidine ethyl ester alone.

[0165] For the target whitefly, samples containing a mixture of compound I:CCXN-15-005-S and compound II: methoxypiperidine ethyl ester at different mass ratios showed 2-3 times higher efficacy than compound I:CCXN-15-005-S alone; samples containing a mixture of compound I:CCXN-15-005-S and compound II: methoxypiperidine ethyl ester at different mass ratios showed 29-125 times higher efficacy than compound II: methoxypiperidine ethyl ester alone.

[0166] The efficacy of the target spider mite, the mixture of compound I:CCXN-15-005-S and compound II: methoxypiperidine ethyl ester at different mass ratios was 3-4 times higher than that of compound I:CCXN-15-005-S alone; the efficacy of the mixture of compound I:CCXN-15-005-S and compound II: methoxypiperidine ethyl ester at different mass ratios was 86-122 times higher than that of compound II: methoxypiperidine ethyl ester alone.

[0167] Target aphids, observed for 48 hours. Samples containing mixtures of compound III:CCXN-15-005 (racemic) and compound II: methoxypiperidine ethyl ester at different mass ratios showed approximately 60% lower efficacy compared to using compound III:CCXN-15-005 (racemic) alone; samples containing mixtures of compound III:CCXN-15-005 (racemic) and compound II: methoxypiperidine ethyl ester at different mass ratios showed approximately 50% higher efficacy compared to using compound II: methoxypiperidine ethyl ester alone.

[0168] Target aphids, observed for 72 hours. Samples containing mixtures of compound III:CCXN-15-005 (racemic) and compound II: methoxypiperidine ethyl ester at different mass ratios showed comparable efficacy to compound III:CCXN-15-005 (racemic) alone. Samples containing mixtures of compound III:CCXN-15-005 (racemic) and compound II: methoxypiperidine ethyl ester at different mass ratios showed approximately twice the efficacy of compound II: methoxypiperidine ethyl ester alone.

[0169] Target aphids, observed for 96 hours. Samples of mixtures of compound III:CCXN-15-005 (racemic) and compound II: methoxypiperidine ethyl ester at different mass ratios showed comparable efficacy to compound III:CCXN-15-005 (racemic) alone.

[0170] For the target whitefly, samples containing mixtures of compound III:CCXN-15-005 (racemic) and compound II: methoxypiperidine ethyl ester at different mass ratios showed approximately 70% lower efficacy compared to using compound III:CCXN-15-005 (racemic) alone; samples containing mixtures of compound III:CCXN-15-005 (racemic) and compound II: methoxypiperidine ethyl ester at different mass ratios showed approximately 20% higher efficacy compared to using compound II: methoxypiperidine ethyl ester alone.

[0171] The efficacy of the target spider mite, the mixture of compound III:CCXN-15-005 (racemic) and compound II: methoxypiperidine ethyl ester at different mass ratios was reduced by about 50% compared to the use of compound III:CCXN-15-005 (racemic) alone; the efficacy of the mixture of compound III:CCXN-15-005 (racemic) and compound II: methoxypiperidine ethyl ester at different mass ratios was increased by about 30% compared to the use of compound II: methoxypiperidine ethyl ester alone.

[0172] Therefore, compared with the use of compound I: CCXN-15-005-S, compound II: methoxypiperidine ethyl ester, or compound alone...

[0173] Compared to III:CCXN-15-005 (racemic mixture), the combination of compound I:CCXN-15-005-S and compound II: methoxypiperidine ethyl ester has significantly better control effects against aphids, whiteflies, and two-spotted spider mites.

[0174] 7. Compound II is flufenoxuron carbonate, and the results are shown in Table 7.

[0175] Table 7. Indoor bioassay results for Compound I: CCXN-15-005-S and Compound II: Flufenoxuron-methyl

[0176]

[0177]

[0178] From Table 7, we can draw the following conclusions:

[0179] For the target aphid, samples of compound I:CCXN-15-005-S and compound II: flufenoxuron in different mass ratios showed 2-3 times higher efficacy than compound I:CCXN-15-005-S alone; samples of compound I:CCXN-15-005-S and compound II: flufenoxuron in different mass ratios showed 11-17 times higher efficacy than compound II: flufenoxuron alone.

[0180] For the target whitefly, samples containing compounds I:CCXN-15-005-S and II: flufenoxuron in different mass ratios showed 3-5 times higher efficacy than compound I:CCXN-15-005-S alone; samples containing compounds I:CCXN-15-005-S and II: flufenoxuron in different mass ratios showed 9-13 times higher efficacy than compound II: flufenoxuron alone.

[0181] For the target thrips, samples of compound I:CCXN-15-005-S and compound II: flufenoxuron in different mass ratios showed 4-9 times greater efficacy than compound I:CCXN-15-005-S alone; samples of compound I:CCXN-15-005-S and compound II: flufenoxuron in different mass ratios showed 17-39 times greater efficacy than compound II: flufenoxuron alone.

[0182] The efficacy of the target spider mite, the mixture of compound I:CCXN-15-005-S and compound II: flufenoxuron in different mass ratios was 3-6 times higher than that of compound I:CCXN-15-005-S alone; the efficacy of the mixture of compound I:CCXN-15-005-S and compound II: flufenoxuron in different mass ratios was 24-58 times higher than that of compound II: flufenoxuron alone.

[0183] Therefore, compared with using compound I:CCXN-15-005-S and compound II: flufenoxuron alone, compound...

[0184] The combination of compound I:CCXN-15-005-S and compound II:flufenicol has a significantly better control effect on aphids, whiteflies, thrips, and two-spotted spider mites.

[0185] 8. Compound II is cypermethrin, and the results are shown in Table 8.

[0186] Table 8. Indoor biomass test results of compound I: CCXN-15-005-S and compound II: cypermethrin

[0187]

[0188]

[0189] From Table 8, we can draw the following conclusions:

[0190] The target species, Tetranychus terrestris (adults + larvae), showed that samples containing a mixture of compound I (CCXN-15-005-S) and compound II (pyridaben) at different mass ratios exhibited 3-5 times greater efficacy than compound I (CCXN-15-005-S) alone.

[0191] Samples containing compound I:CCXN-15-005-S and compound II: cyprodinil at different mass ratios showed 33-48 times greater efficacy than compound II: cyprodinil alone.

[0192] The target, Tetranychus terrestris (eggs), showed that samples containing a mixture of compound I: CCXN-15-005-S and compound II: cyprodinil at different mass ratios were 5-6 times more effective than compound I: CCXN-15-005-S alone; samples containing a mixture of compound I: CCXN-15-005-S and compound II: cyprodinil at different mass ratios were 46-52 times more effective than compound II: cyprodinil alone.

[0193] The target species, Tetranychus kamizawa (adults + larvae), showed that samples containing a mixture of compound I (CCXN-15-005-S) and compound II (pyridaben) at different mass ratios exhibited 3-7 times greater efficacy than compound I (CCXN-15-005-S) alone.

[0194] Samples containing compound I:CCXN-15-005-S and compound II: cypermethrin at different mass ratios showed 27-58 times greater efficacy than compound II: cypermethrin alone.

[0195] The target, Tetranychus kamizawa (eggs), showed that samples containing a mixture of compound I (CCXN-15-005-S) and compound II (pyridaben) at different mass ratios exhibited 4-6 times greater efficacy than compound I (CCXN-15-005-S) alone.

[0196] Samples containing compound I:CCXN-15-005-S and compound II:pyridaben at different mass ratios showed 48-68 times greater efficacy than compound II:pyridaben alone.

[0197] For the target citrus psyllid (adults + larvae), samples containing a mixture of compound I: CCXN-15-005-S and compound II: cyprodinil at different mass ratios showed 2-4 times higher efficacy compared to using compound I: CCXN-15-005-S alone;

[0198] Samples containing compound I:CCXN-15-005-S and compound II: cyprodinil at different mass ratios showed 15-30 times greater efficacy than compound II: cyprodinil alone.

[0199] The target, *Paecilomyces citrus* (eggs), showed that samples containing a mixture of compound I (CCXN-15-005-S) and compound II (pyridaben) at different mass ratios exhibited 2-4 times greater efficacy than compound I (CCXN-15-005-S) alone.

[0200] Samples containing compound I:CCXN-15-005-S and compound II:pyridaben at different mass ratios showed 20-33 times greater efficacy than compound II:pyridaben alone.

[0201] Therefore, compared with using compound I:CCXN-15-005-S and compound II: cypermethrin alone, compound...

[0202] The combination of compound I:CCXN-15-005-S and compound II:pyridaben has a significantly better control effect on two-spotted spider mites, kasugamys spider mites, and citrus psyllids.

[0203] 9. Compound II is pyrazolyl aniline, and the results are shown in Table 9.

[0204] Table 9. Indoor bioassay results for Compound I: CCXN-15-005-S and Compound II: Pyrazolamide

[0205]

[0206] From Table 9, we can draw the following conclusions:

[0207] The target spider mite (adults + larvae), samples containing a mixture of compound I: CCXN-15-005-S and compound II: pyrazolylaniline at different mass ratios showed 3-5 times higher efficacy compared to using compound I: CCXN-15-005-S alone;

[0208] Samples containing compound I:CCXN-15-005-S and compound II:pyrazolamide at different mass ratios showed 15-27 times greater efficacy than compound II:pyrazolamide alone.

[0209] The target, *Tetranychus spp.* (eggs), showed that a mixture of compound I (CCXN-15-005-S) and compound II (pyrazolylaniline) at different mass ratios was 4-6 times more effective than compound I (CCXN-15-005-S) alone.

[0210] Samples containing compound I:CCXN-15-005-S and compound II:pyrazolamide at different mass ratios showed 270-386 times greater efficacy than compound II:pyrazolamide alone.

[0211] For the target citrus psyllid (adults + larvae), samples containing a mixture of compound I: CCXN-15-005-S and compound II: pyrazolylaniline at different mass ratios showed 2-6 times higher efficacy compared to using compound I: CCXN-15-005-S alone;

[0212] Samples containing compound I:CCXN-15-005-S and compound II:pyrazolamide at different mass ratios showed 17-47 times greater efficacy than compound II:pyrazolamide alone.

[0213] The target, *Pachycerium citrinum* (eggs), showed that samples containing a mixture of compound I (CCXN-15-005-S) and compound II (pyrazolylaniline) at different mass ratios exhibited 2-3 times greater efficacy than compound I (CCXN-15-005-S) alone.

[0214] Samples containing compound I:CCXN-15-005-S and compound II:pyrazolamide at different mass ratios showed 38-59 times greater efficacy than compound II:pyrazolamide alone.

[0215] Therefore, compared with using compound I:CCXN-15-005-S and compound II:pyrazolylbenzene alone, compound

[0216] The combination of compound I:CCXN-15-005-S and compound II:pyrazolylbenzene has a significantly better control effect on two-spotted spider mite and citrus psyllid.

[0217] 10. Compound II is etoxazole, and the results are shown in Table 10.

[0218] Table 10. Indoor biodiversity test results of compound I: CCXN-15-005-S and compound II: etoxazole; Indoor biodiversity test results of compound III: CCXN-15-005 (racemic mixture) and compound II: etoxazole.

[0219]

[0220] From Table 10, we can draw the following conclusions:

[0221] The target spider mite (adults + larvae), samples containing a mixture of compound I: CCXN-15-005-S and compound II: etoxazole at different mass ratios showed 3-4 times higher efficacy compared to using compound I: CCXN-15-005-S alone;

[0222] Samples containing compound I:CCXN-15-005-S and compound II: etoxazole at different mass ratios showed 4-5 times greater efficacy than compound II: etoxazole alone.

[0223] For the target Tetranychus terrestris (eggs), samples containing a mixture of compound I:CCXN-15-005-S and compound II: etoxazole at different mass ratios showed a 3-5 times higher efficacy compared to using compound I:CCXN-15-005-S alone; samples containing a mixture of compound I:CCXN-15-005-S and compound II: etoxazole at different mass ratios showed a 7-11 times higher efficacy compared to using compound II: etoxazole alone.

[0224] The target spider mite (adults + larvae) showed comparable efficacy when compound III:CCXN-15-005 (racemic mixture) and compound II: etoxazole were mixed in different mass ratios compared to the use of compound III:CCXN-15-005 (racemic mixture) alone. However, the efficacy of the mixed compound III:CCXN-15-005 (racemic mixture) and compound II: etoxazole was reduced by approximately 80% compared to the use of compound II: etoxazole alone.

[0225] The target Tetranychus erythrorhizoma (eggs) showed comparable efficacy to the mixture of compound III:CCXN-15-005 (racemate) and compound II: etoxazole at different mass ratios compared to the use of compound III:CCXN-15-005 (racemate) alone. However, the efficacy of the mixture of compound III:CCXN-15-005 (racemate) and compound II: etoxazole at different mass ratios was reduced by approximately 80% compared to the use of compound II: etoxazole alone.

[0226] Therefore, compared with using compound I:CCXN-15-005-S and compound II: etoxazole alone, compound

[0227] The combination of compound I:CCXN-15-005-S and compound II: etoxazole exhibits significantly better control efficacy against two-spotted spider mites.

[0228] 11. Compound II is acaricide quinone, and the results are shown in Table 11.

[0229] Table 11: Indoor biomass test results of compound I: CCXN-15-005-S and compound II: acaricide quinone

[0230]

[0231]

[0232] From Table 11, we can draw the following conclusions:

[0233] The target spider mite (adults + larvae), samples containing a mixture of compound I: CCXN-15-005-S and compound II: acaricide at different mass ratios showed a 3-fold increase in efficacy compared to using compound I: CCXN-15-005-S alone;

[0234] Samples containing compound I:CCXN-15-005-S and compound II: miticide in different mass ratios showed 19-23 times greater efficacy than compound II: miticide alone.

[0235] The target, Tetranychus dichotoma (eggs), showed that samples containing a mixture of compound I:CCXN-15-005-S and compound II: acaricide at different mass ratios were 4-5 times more effective than compound I:CCXN-15-005-S alone; samples containing a mixture of compound I:CCXN-15-005-S and compound II: acaricide at different mass ratios were 25-34 times more effective than compound II: acaricide alone.

[0236] The target spider mite (adults + larvae), samples containing a mixture of compound I: CCXN-15-005-S and compound II: acaricide at different mass ratios showed twice the efficacy of compound I: CCXN-15-005-S alone against the target spider mite (adults + larvae);

[0237] Samples containing compound I:CCXN-15-005-S and compound II: miticide in different mass ratios showed 45-54 times greater efficacy than compound II: miticide alone.

[0238] The target, Tetranychus kanazei (eggs), showed that samples containing a mixture of compound I: CCXN-15-005-S and compound II: acaricide at different mass ratios were 2-3 times more effective than compound I: CCXN-15-005-S alone; samples containing a mixture of compound I: CCXN-15-005-S and compound II: acaricide at different mass ratios were 31-49 times more effective than compound II: acaricide alone.

[0239] The target species, *Pseudomonas citrus* (adults + larvae), showed that samples containing a mixture of compound I (CCXN-15-005-S) and compound II (tebufenozide) at different mass ratios exhibited 2-3 times greater efficacy than compound I (CCXN-15-005-S) alone.

[0240] Samples containing compound I:CCXN-15-005-S and compound II: miticide in different mass ratios showed 19-25 times greater efficacy than compound II: miticide alone.

[0241] The target, *Pachycerium citrinum* (eggs), showed that samples containing a mixture of compound I: CCXN-15-005-S and compound II: acaricide at different mass ratios were 4 times more effective than compound I: CCXN-15-005-S alone; samples containing a mixture of compound I: CCXN-15-005-S and compound II: acaricide at different mass ratios were 19-23 times more effective than compound II: acaricide alone.

[0242] Therefore, compared with using compound I:CCXN-15-005-S and compound II: acaricide alone, compound

[0243] The combination of compound I:CCXN-15-005-S and compound II: acaricide quinone has a significantly better control effect on two-spotted spider mite, senzhe's spider mite, and citrus psyllid.

[0244] 12. Compound II is flufenoxuron, and the results are shown in Table 12.

[0245] Table 12: Indoor biomass test results of compound I: CCXN-15-005-S and compound II: flufenoxuron

[0246]

[0247] From Table 12, we can draw the following conclusions:

[0248] The target spider mite (adults + larvae), samples containing a mixture of compound I: CCXN-15-005-S and compound II: flufenoxuron at different mass ratios showed 2-3 times higher efficacy compared to using compound I: CCXN-15-005-S alone;

[0249] Samples containing compound I:CCXN-15-005-S and compound II:flufenicol at different mass ratios showed 9-11 times greater efficacy than compound II:flufenicol alone.

[0250] The target, Tetranychus terrestris (eggs), showed that samples containing a mixture of compound I: CCXN-15-005-S and compound II: flufenoxuron at different mass ratios were 3 times more effective than compound I: CCXN-15-005-S alone; samples containing a mixture of compound I: CCXN-15-005-S and compound II: flufenoxuron at different mass ratios were 123-144 times more effective than compound II: flufenoxuron alone.

[0251] The target species, *Pseudomonas citrus* (adults + larvae), showed that samples containing a mixture of compound I (CCXN-15-005-S) and compound II (flufenicol) at different mass ratios exhibited 2-3 times greater efficacy than compound I (CCXN-15-005-S) alone.

[0252] Samples containing compound I:CCXN-15-005-S and compound II:flufenicol at different mass ratios showed 9-14 times greater efficacy than compound II:flufenicol alone.

[0253] The target, *Pteris vittata* (eggs), showed that samples containing a mixture of compound I (CCXN-15-005-S) and compound II (flufenicol) at different mass ratios were 2-6 times more effective than compound I (CCXN-15-005-S) alone; samples containing a mixture of compound I (CCXN-15-005-S) and compound II (flufenicol) at different mass ratios were 37-101 times more effective than compound II (flufenicol) alone.

[0254] Therefore, compared with using compound I:CCXN-15-005-S and compound II: flufenoxuron alone, compound...

[0255] The combination of compound I:CCXN-15-005-S and compound II:flufenicol has a significantly better control effect on two-spotted spider mites and citrus pterocaryon.

[0256] 13. Compound II is flufenoxuron, and the results are shown in Table 13.

[0257] Table 13. Indoor biodiversity test results of compound I: CCXN-15-005-S and compound II: flufenoxuron.

[0258]

[0259] From Table 13, we can draw the following conclusions:

[0260] The target spider mite (adults + larvae), samples containing a mixture of compound I: CCXN-15-005-S and compound II: flufenoxuron at different mass ratios showed 3-6 times higher efficacy compared to using compound I: CCXN-15-005-S alone;

[0261] Samples containing compound I:CCXN-15-005-S and compound II:flufenoxam at different mass ratios showed 6-10 times greater efficacy than compound II:flufenoxam alone.

[0262] The target, Tetranychus taeniatus (eggs), showed that samples containing a mixture of compound I (CCXN-15-005-S) and compound II (flufenoxam) at different mass ratios exhibited 6-9 times greater efficacy than compound I (CCXN-15-005-S) alone.

[0263] Samples containing compound I:CCXN-15-005-S and compound II:flufenoxam at different mass ratios showed 9-13 times greater efficacy than compound II:flufenoxam alone.

[0264] The target spider mite (adults + larvae), samples containing a mixture of compound I: CCXN-15-005-S and compound II: flufenoxuron at different mass ratios showed 2-3 times higher efficacy compared to using compound I: CCXN-15-005-S alone;

[0265] Samples containing compound I:CCXN-15-005-S and compound II:flufenoxam at different mass ratios showed 4-6 times greater efficacy than compound II:flufenoxam alone.

[0266] The target, Tetranychus kamizawa (eggs), showed that samples containing a mixture of compound I (CCXN-15-005-S) and compound II (flufenoxam) at different mass ratios exhibited 4-6 times greater efficacy than compound I (CCXN-15-005-S) alone.

[0267] Samples containing compound I:CCXN-15-005-S and compound II:flufenoxam at different mass ratios showed 4-5 times greater efficacy than compound II:flufenoxam alone.

[0268] The target species, *Pseudomonas citrus* (adults + larvae), showed that samples containing a mixture of compound I (CCXN-15-005-S) and compound II (flufenoxam) at different mass ratios exhibited 3-6 times greater efficacy than compound I (CCXN-15-005-S) alone.

[0269] Samples containing compound I:CCXN-15-005-S and compound II:flufenoxam at different mass ratios showed 4-8 times greater efficacy than compound II:flufenoxam alone.

[0270] The target, *Paecilomyces citrus* (eggs), showed that samples containing a mixture of compound I (CCXN-15-005-S) and compound II (flufenoxam) at different mass ratios exhibited 4-6 times greater efficacy than compound I (CCXN-15-005-S) alone.

[0271] Samples containing compound I:CCXN-15-005-S and compound II:flufenoxam at different mass ratios showed 5-7 times greater efficacy than compound II:flufenoxam alone.

[0272] Therefore, compared with using compound I:CCXN-15-005-S and compound II: flufenoxuron alone, compound...

[0273] The combination of compound I:CCXN-15-005-S and compound II:flufenoxam has a significantly better control effect on two-spotted spider mites, kasugamys spider mites, and citrus psyllids.

[0274] While the specific embodiments of the present invention have been described above in conjunction with the accompanying drawings, this is not intended to limit the scope of protection of the present invention. Based on the technical solutions of the present invention, various modifications or variations that can be made by those skilled in the art without creative effort are still within the scope of protection of the present invention.

Claims

1. A synergistic composition comprising an isoxazoline insecticide / acaricide, characterized in that, consists of compound I and compound II, the compound I is CCXN-15-005-S or its agrochemically acceptable salt, the compound II is selected from any one of the following: a nicotinic acetylcholine receptor agonist, a chordophone TRPV channel modulator, an acetyl-CoA carboxylase inhibitor, a mitochondrial electron transport inhibitor, a calcium-activated potassium ion channel modulator, a trifluoroethyl sulfide (sulfoxide) or its agrochemically acceptable salt; wherein the chemical name of CCXN-15-005-S is (S)-4-(5-(3,5-dichloro-4-fluorophenyl)-5-(trifluoromethyl)-4,5-dihydroisoxazol-3-yl)-N-((methoxyimino)methyl)-2-methylbenzamide, and the chemical structural formula is as follows:

2. The composition of claim 1, wherein, The mass ratio of the compound I and the compound II is 1:50-50:

1.

3. The composition of claim 1, wherein, The nicotinic acetylcholine receptor agonist is selected from any one of the following: acetamiprid, imidacloprid, thiamethoxam, nitenpyram, dinotefuran, clothianidin, thiacloprid, chloropyrifos, pyridalyl, pyridaben, chlorfenapyr, flonicamid, sulfoxaflor, flupyradifurone.

4. The composition of claim 1, wherein, The chordophone TRPV channel modulator is selected from any one of the following: flupyradifurone, sulfoxaflor, pyridalyl.

5. The composition of claim 1, wherein, The acetyl-CoA carboxylase inhibitor is selected from spirotetramat or etoxazole.

6. The composition of claim 1, wherein, The mitochondrial electron transport inhibitor is selected from any one of the following: fluensulfone, clofentezine, pyridaben, diafenthiuron, acequinocyl, bifenazate, pyridifen, pyridylphen, fenpyroximate, chinomethionat, pyrimidifen, pyriminostrobin, tolfenpyrad.

7. The composition of claim 1, wherein, The calcium-activated potassium ion channel modulator is fluacrypyrim.

8. The composition of claim 1, wherein, The trifluoroethyl sulfide (sulfoxide) is fluensulfone.

9. Use of the composition of any one of claims 1-8 in the preparation of an agricultural insecticide and acaricide.

10. An agricultural insecticide-acaricide characterized by comprising, The composition of any one of claims 1-8 is contained.

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