Traditional Chinese medicine emulsion for relieving and excreting acid and application of traditional Chinese medicine emulsion

Abstract

The invention relates to a traditional Chinese medicine emulsion for relieving and excreting acid and application thereof, and relates to the technical field of traditional Chinese medicines. The active pharmaceutical ingredients of the traditional Chinese medicine emulsion comprise an angelica sinensis extract, a centella asiatica extract, a sea-buckthorn extract, a mint extract and a ginger extract. The traditional Chinese medicine emulsion has a remarkable treatment effect on soreness and swelling caused by excessive lactic acid in body muscles and lactic acid nodules, particularly can be used for targeted treatment on soreness and swelling of shoulders, necks and waists, and has the effects of treating both symptoms and root causes and solving the root cause during treatment.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of traditional Chinese medicine, in particular to a traditional Chinese medicine emulsion for relieving acid discharge and application thereof. BACKGROUND

[0002] Our muscles are made up of long, thin muscle fibers. If posture and movement are not proper for a long time, or if there is intense or excessive exercise, some muscle fibers will be in a state of tension all the time, the pressure in the muscle increases, and the blood supply is blocked. In this way, the energy consumed by the muscle fibers when contracting cannot be replenished, and toxic substances called lactic acid are secreted. Excessive lactic acid can easily cause muscle fatigue and soreness. When the body cannot metabolize the generated lactic acid in time, the lactic acid will accumulate in the muscle cells and form tiny crystalline bodies. These crystalline bodies can stimulate the surrounding tissues, triggering an inflammatory response and a fibrosis process, thereby forming qi stagnation and blood stasis, lactic acid nodules, which is also called wind, cold and dampness in traditional Chinese medicine. This congestion nodule can further affect the tissues and nerves, making the pain symptoms worse.

[0003] The causes of lactic acid nodules are diverse, but generally have factors such as chronic strain, acute injury, and long-term fatigue: (1) Chronic strain is one of the important causes of lactic acid nodules. Muscles and ligaments are prone to chronic injury when they are repeatedly subjected to the same movement or maintain the same action for a long time. This injury can lead to the accumulation of lactic acid in the local area, which in turn stimulates the thickening and hardening of the muscle and ligament, eventually forming lactic acid nodules; (2) Acute injury is also a common cause of lactic acid nodules. Intense exercise and heavy physical labor can cause partial muscle fiber strain, generating a large amount of lactic acid and other acidic metabolites. These metabolites can stimulate the damaged muscle fibers to develop aseptic inflammation, accompanied by edema and exudation, and eventually form lactic acid nodules in the local area; (3) Long-term fatigue can also cause lactic acid nodules. When the body is in a state of fatigue for a long time, lactic acid and other metabolites tend to accumulate in the muscles, especially in the shoulder and neck. If this accumulation is not relieved for a long time, lactic acid nodules will gradually form.

[0004] Excessive lactic acid and lactic acid nodules in the human body can easily cause decreased exercise capacity, muscle soreness, and severe problems such as numbness and cramps in the limbs, periarthritis of the shoulder, rich and noble package, and lumbar disc herniation. Pain in the shoulder, neck, waist and limbs is one of the most common symptoms of excessive lactic acid and lactic acid nodules. Currently, external drugs on the market for treating neck, waist and limb pain, such as liniments, ointments and patches, can eliminate inflammation and relieve pain symptoms. However, for lactic acid and lactic acid nodules causing pain in the shoulder, neck, waist and limbs, these drugs only treat the symptoms and not the root cause, so the pain will soon recur. SUMMARY

[0005] In view of the above problems, the present application provides a traditional Chinese medicine emulsion for relieving acid discharge, which has a remarkable therapeutic effect on the acid pain and swelling caused by excessive lactic acid in the body muscles and lactic acid nodules, especially can treat the acid pain and swelling of the shoulder, neck, waist and limbs in a targeted manner, and has the effect of treating the symptoms and the root cause of the disease.

[0006] The present application provides a traditional Chinese medicine emulsion for relieving acid discharge, which comprises a pharmaceutical active ingredient and a pharmaceutically acceptable auxiliary material, and the weight ratio of the pharmaceutical active ingredient to the auxiliary material is (0.8-13):(13-60.5); the pharmaceutical active ingredient comprises the following raw materials in a weight ratio of:

[0007]

[0008]

[0009] The inventors have found that the conventional analgesics cannot effectively treat the pain or swelling caused by excessive lactic acid and lactic acid nodules because the conventional analgesics mainly use traditional Chinese medicines, methyl salicylate or hormones as main components for anti-inflammatory and analgesic effects, and cannot effectively remove acid and remove nodules, so that after the patients use the conventional analgesics, the analgesic and anti-inflammatory effects can be achieved in a short time, but the problem of lactic acid and nodules in the body cannot be solved in substance, and therefore the pain and swelling will recur after a period of time. Based on this, the inventors propose the above-mentioned raw material combination to form a traditional Chinese medicine emulsion. The angelica extract can tonify blood and activate blood, dredge channels and collaterals, has the effects of anti-inflammatory, analgesic and promoting blood circulation, the main active components are ligustilide and angelica polysaccharide, can dilate blood vessels, improve local microcirculation, make inflammatory factors metabolize faster, thereby reduce pain, inhibit platelet aggregation, reduce blood viscosity, improve blood circulation, prevent thrombosis, and prevent the formation of nodules caused by blood stasis. The extract of ginkgo leaf has the effects of clearing heat and removing dampness, detoxifying and detumescence, the main active components are bilobalide, ginkgolide B, ginkgolide C, ginkgolide J, and can relieve symptoms such as contusion and swelling, and reduce the degree of muscle fibrosis, and repair muscle cells of sports injury. The extract of sea buckthorn contains rich flavonoids and unsaturated fatty acids, can enhance the activity of cells, accelerate metabolism, promote the excretion of muscle acidic substances, and is a natural antioxidant, which can help remove oxygen free radicals in the body, thereby delaying cell degeneration. The ginger extract is a traditional Chinese medicine with the effects of warming meridians, dispelling cold and dampness, promoting blood circulation and relieving pain. It mainly contains active components such as gingerol, gingerol, gingerol, and curcumin, can reduce muscle injury and inflammation after intense exercise, reduce the influence of delayed muscle soreness caused by exercise, can help blood circulation, and can help the slow dispersion of nodules accumulated in the body. The peppermint extract can help the effective components penetrate the skin to form a "skin reservoir", so that the essential oil can better enter the dermis layer. Compared with chemical penetration agents such as azone, the peppermint essential oil is a pure natural component, can change the permeability of the stratum corneum, reduce the resistance of the effective components to the skin absorption, and increase the diffusion coefficient of the components.

[0010] In one embodiment, the pharmaceutically active ingredients include the following raw materials in parts by weight:

[0011]

[0012] In one embodiment, the pharmaceutically active ingredients include the following raw materials in parts by weight:

[0013]

[0014] In one embodiment, the excipients include the following raw materials in parts by weight:

[0015]

[0016] In one embodiment, the adjuvant includes the following raw materials in parts by weight:

[0017]

[0018] In one embodiment, the adjuvant includes the following raw materials in parts by weight:

[0019]

[0020] The application also provides a preparation method of the traditional Chinese medicine emulsion, including the following steps: mixing raw materials of a drug active ingredient and part of raw materials of an adjuvant to obtain a first mixed solution, mixing the remaining raw materials of the adjuvant at a speed of 500-700 rpm at 45-55℃ to obtain a second mixed solution, and mixing the first mixed solution and the second mixed solution at a speed of 500-700 rpm at 45-55℃.

[0021] In one embodiment, the preparation method includes the following steps: mixing raw materials of a drug active ingredient and water, propylene glycol, glycerol, angelica extract, and ethylhexyl palmitate to obtain a first mixed solution, mixing cetyl stearyl alcohol, glyceryl stearate, dimethicone, carbomer, sodium acrylate / sodium acryloyldimethyl taurate copolymer, and polyacrylamide at a speed of 600 rpm at 50℃ for 1h to obtain a second mixed solution, mixing the first mixed solution and the second mixed solution at a speed of 600 rpm at 50℃ for 2h, and cooling to room temperature.

[0022] The application also provides an application of the traditional Chinese medicine emulsion in preparing a medicine for expelling acid and resolving nodes.

[0023] The application also provides an application of the traditional Chinese medicine emulsion in preparing a medicine for relieving pain and / or swelling.

[0024] In one embodiment, the medicine for relieving pain and / or swelling is a medicine for relieving or eliminating pain and / or swelling caused by muscle lactic acid and lactic acid nodules.

[0025] In one embodiment, the pain or swelling is located in the shoulder, neck or waist.

[0026] The application also provides a medicine for expelling acid and resolving nodes, including an effective component and a pharmaceutically acceptable adjuvant, wherein the effective component includes the traditional Chinese medicine emulsion.

[0027] The medicine for acid elimination and knot dissipation with the above traditional Chinese medicine emulsion as the effective component has the following effects: (1) eliminating muscle lactic acid, enhancing cell activity through the sea buckthorn extract, accelerating metabolism, and promoting rapid elimination of muscle acidic substances; (2) preventing lactic acid nodules, reducing the degree of muscle fibrosis through the centella asiatica extract, preventing qi stagnation and blood stasis and lactic acid nodules; (3) eliminating wind, cold and dampness, dredging meridians through the angelica extract and ginger extract, warming yang and dispelling cold, eliminating cold, improving local microcirculation, helping to dissipate qi stagnation and blood stasis and lactic acid nodules, and avoiding rebound of pain symptoms; (4) eliminating inflammation and relieving pain, eliminating inflammation through the centella asiatica extract and ginger extract, and quickly relieving pain; (5) repairing damaged muscle cells, protecting cells from free radicals through the strong antioxidant and repair effects of the sea buckthorn extract and the centella asiatica extract, promoting blood circulation, and effectively promoting muscle cell regeneration; (6) removing blood stasis and dissolving nodules, dredging meridians and softening and dissolving nodules through the sea buckthorn extract, the angelica extract and the ginger extract, which can accelerate the slow dispersion of internal accumulated nodules and effectively remove blood stasis and dissolve nodules; (7) promoting drug penetration, helping the effective components penetrate the skin through the peppermint essential oil, and allowing the essential oil to better enter the dermis. The six drugs work together to activate blood circulation, regulate qi, warm the meridians, dispel cold, eliminate inflammation and relieve pain, eliminate lactic acid, remove blood stasis and dissolve nodules, and are suitable for the pain and swelling problems of the shoulder, neck, waist and limbs caused by excessive muscle lactic acid and lactic acid nodules.

[0028] In one of the embodiments, the medicine for acid elimination and knot dissipation is in the form of serum, gel, liniment, spray or tincture.

[0029] The application further provides a medicine for pain relief and / or swelling reduction, which comprises effective components and pharmaceutically acceptable adjuvants, wherein the effective components comprise the traditional Chinese medicine emulsion.

[0030] In one of the embodiments, the medicine for pain relief and / or swelling reduction is in the form of serum, gel, liniment, spray or tincture.

[0031] Compared with the prior art, the application has the following beneficial effects:

[0032] The traditional Chinese medicine emulsion for relieving acid elimination and its application can synergistically act on the raw materials, has a strong effect of acid elimination and knot dissipation, and is more targeted, can fundamentally treat the pain and / or swelling caused by excessive muscle lactic acid and lactic acid nodules, repair damaged tissues, restore normal functions of the body, especially the pain and swelling problems of the shoulder, neck, waist and limbs, and has a significant therapeutic effect. Compared with conventional external preparations, the traditional Chinese medicine emulsion has obvious curative effect, significantly improves the symptoms of patients, has few adverse reactions, and has no rebound and recurrence, and is safe and convenient to use and has few side effects. BRIEF DESCRIPTION OF DRAWINGS

[0033] Figure 1The front and back contrast chart of the patient using the product of the present application in example 1 on the neck;

[0034] Figure 2 The front and back contrast chart of the patient using the product of the present application in example 1 on the shoulder. DETAILED DESCRIPTION

[0035] In order to facilitate the understanding of the present application, the present application will be described more fully below with reference to the accompanying drawings. The preferred embodiments of the present application are shown in the drawings. However, the present application can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the disclosure of the present application more thorough and comprehensive.

[0036] Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which the present application belongs. The terminology used in the specification of the present application herein is only for the purpose of describing specific embodiments and is not intended to limit the present application. The term "and / or" used herein includes any and all combinations of one or more related listed items.

[0037] The reagents, materials and equipment used in the present application are commercially available unless otherwise specified. The test methods are conventional test methods in the art unless otherwise specified.

[0038] Example 1

[0039] The present application provides a kind of external use traditional Chinese medicine emulsion for acid discharge and knot, and the traditional Chinese medicine compound essential oil is made of the following weight parts of raw materials: water 25 parts, emu oil 20 parts, angelica extract 1 part, centella asiatica extract 1 part, sea buckthorn extract 1 part, mint extract 0.4 parts, ginger extract 0.5 parts, propylene glycol 6 parts, glycerol 5 parts, ethylhexyl palmitate 1 part, cetyl stearyl alcohol 1.5 parts, glyceryl stearate 0.8 parts, dimethicone 0.5 parts, carbomer 0.15 parts, sodium acrylate / sodium acryloyldimethyl taurate copolymer 0.2 parts, polyacrylamide 0.15 parts.

[0040] A kind of method for preparing the traditional Chinese medicine emulsion, by the following steps consists of steps:

[0041] (1) first water, emu oil, propylene glycol, glycerol, angelica extract, centella asiatica extract, sea buckthorn extract, mint extract, ginger extract, ethylhexyl palmitate, the above raw materials are mixed and stirred uniformly, and the mixed solution is obtained;

[0042] (2) again add cetyl stearyl alcohol, glyceryl stearate, dimethicone, carbomer, sodium acrylate / sodium acryloyldimethyl taurate copolymer, polyacrylamide to the mixture, mix them, use a disperser to mix at 50℃ for 1h at 600rpm, then obtain a mixture;

[0043] (3) add the mixture obtained in step (1) to the mixture in step (2), then mix again at 50℃ for 2h at 600rpm, and cool to room temperature to obtain a finished product, a white cream.

[0044] Example 2

[0045] The present application provides a traditional Chinese medicine emulsion for acid discharge and knot dissolution, which comprises the following raw materials by weight: water 5 parts, emu oil 20 parts, angelica extract 0.5 parts, gotu kola extract 0.4 parts, sea buckthorn extract 0.4 parts, mint extract 0.2 parts, ginger extract 0.1 part, propylene glycol 5 parts, glycerol 4 parts, ethylhexyl palmitate 1 part, cetyl stearyl alcohol 1 part, glyceryl stearate 0.6 parts, dimethicone 0.3 parts, carbomer 0.1 part, sodium acrylate / sodium acryloyldimethyl taurate copolymer 0.1 part, and polyacrylamide 0.1 part.

[0046] A method for preparing the traditional Chinese medicine emulsion, which comprises the following steps:

[0047] (1) first mix water, emu oil, propylene glycol, glycerol, angelica extract, gotu kola extract, sea buckthorn extract, mint extract, ginger extract, and ethylhexyl palmitate, and stir them evenly to obtain a mixture;

[0048] (2) again add cetyl stearyl alcohol, glyceryl stearate, dimethicone, carbomer, sodium acrylate / sodium acryloyldimethyl taurate copolymer, and polyacrylamide to the mixture, mix them, use a disperser to mix at 50℃ for 1h at 600rpm, then obtain a mixture;

[0049] (3) add the mixture obtained in step (1) to the mixture in step (2), then mix again at 50℃ for 2h at 600rpm, and cool to room temperature to obtain a finished product, a white cream.

[0050] Example 3

[0051] The application provides a traditional Chinese medicine emulsion for discharging acid and resolving stagnation, which comprises the following raw materials in parts by weight: water 30 parts, emu oil 8 parts, angelica extract 1 part, centella asiatica extract 2 parts, hippophae rhamnoides extract 2 parts, mint extract 1 part, ginger extract 1 part, propylene glycol 8 parts, glycerol 8 parts, ethylhexyl palmitate 2 parts, cetyl stearyl alcohol 2 parts, glyceryl stearate 1 part, dimethicone 0.8 parts, carbomer 0.3 parts, sodium acrylate / sodium acryloyldimethyl taurate copolymer 0.3 parts and polyacrylamide 0.3 parts.

[0052] A method for preparing the traditional Chinese medicine emulsion, which comprises the following steps:

[0053] (1) first, mix and stir the following raw materials: water, emu oil, propylene glycol, glycerol, angelica extract, centella asiatica extract, hippophae rhamnoides extract, mint extract, ginger extract and ethylhexyl palmitate, to obtain a mixed solution;

[0054] (2) then, mix the following raw materials: cetyl stearyl alcohol, glyceryl stearate, dimethicone, carbomer, sodium acrylate / sodium acryloyldimethyl taurate copolymer and polyacrylamide, and then use a disperser to mix them at 50 DEG C and 600 rpm for 1 h, to obtain a mixed solution;

[0055] (3) then, add the mixed solution obtained in step (1) into the mixed solution in step (2), and then mix them again at 50 DEG C and 600 rpm for 2 h, and then cool to room temperature to obtain the finished product, a white cream.

[0056] Example 4

[0057] The application provides a traditional Chinese medicine emulsion for discharging acid and resolving stagnation, which comprises the following raw materials in parts by weight: water 20 parts, emu oil 20 parts, angelica extract 1 part, centella asiatica extract 1 part, hippophae rhamnoides extract 1 part, mint extract 1 part, ginger extract 1 part, propylene glycol 5 parts, glycerol 5 parts, ethylhexyl palmitate 2 parts, cetyl stearyl alcohol 2 parts, glyceryl stearate 1 part, dimethicone 0.8 parts, carbomer 0.1 parts, sodium acrylate / sodium acryloyldimethyl taurate copolymer 0.1 parts and polyacrylamide 0.1 parts.

[0058] A method for preparing the traditional Chinese medicine emulsion, which comprises the following steps:

[0059] (1) first, mix and stir the following raw materials: water, emu oil, propylene glycol, glycerol, angelica extract, centella asiatica extract, hippophae rhamnoides extract, mint extract, ginger extract and ethylhexyl palmitate, to obtain a mixed solution;

[0060] (2) Again, the cetyl stearyl alcohol, glyceryl stearate, dimethicone, carbomer, sodium acrylate / sodium acryloyldimethyl taurate copolymer, polyacrylamide, after mixing, using a disperser at 50°C, 600 rpm for 1 h, the mixture was obtained;

[0061] (3) Again, the mixture in step (2) was added to the mixture obtained in step (1), then mixed at 50°C, 600 rpm for 2 h, and cooled to room temperature to obtain the finished product, a white cream.

[0062] Application Example One

[0063] Test Group 1: the product of Example 1;

[0064] Test Group 2: the product of Example 2;

[0065] Test Group 3: the product of Example 3;

[0066] Test Group 4: the product of Example 4;

[0067] Test Group 5: on the basis of Example 1, remove the sea buckthorn extract, and other conditions are consistent with Example 1;

[0068] Test Group 6: on the basis of Example 1, remove the angelica extract, and other conditions are consistent with Example 1;

[0069] Test Group 7: on the basis of Example 1, remove the centella asiatica extract, and other conditions are consistent with Example 1;

[0070] Test Group 8: on the basis of Example 1, remove the ginger extract, and other conditions are consistent with Example 1;

[0071] Test Group 9: on the basis of Example 1, remove the mint extract, and other conditions are consistent with Example 1.

[0072] Cell repair test and results.

[0073] 1. Effect on myoblast C2C12 proliferation.

[0074] The products prepared in the above examples and comparative examples were respectively configured into 1% volume percentage test solution in serum-free DMEM medium. Myoblast C2C12 cells were cultured in DMEM medium containing 10% fetal bovine serum and 1% double antibody (1 x 10 12 5 ​Cells were cultured in DMEM medium containing 100 mg / L penicillin and 100 mg / L streptomycin. Cells were grown in an incubator at 37°C and 5% CO2 saturated humidity. When cell confluence reached 85% or higher, the logarithmic growth phase cells were digested with 0.05% trypsin, and the digestion was terminated with serum-containing DMEM. Cells were counted using a cell counting chamber, and the cell suspension concentration was adjusted to 7 × 10⁻⁶ cells / mL. 4 Cells were seeded at a rate of 100 μL per well in 96-well plates and incubated at 37°C and 5% CO2 for 12 h. The old culture medium was removed, and the cells were washed twice with phosphate-buffered saline (PBS). In the experimental groups, 100 μL of the filtered and sterilized test solutions of different concentrations were added to each well, with three replicates per test solution. The control group contained cells and was inoculated with serum-free DMEM medium; the blank control group contained no cells and was inoculated with 100 μL of PBS. The cells were then incubated at 37°C and 5% CO2 for 24 h. Then, 10 μL of CCK-8 solution was added to each well, and the cells were incubated for another 3 h. The absorbance was measured at 450 nm, and the cell viability was calculated. The results are shown in Table 1.

[0075] Cell viability (%) = (A experimental group - A blank control group) / (A control group - A blank control group) × 100%.

[0076] Table 1 shows the effects of C2C on myoblasts. 12 proliferation analysis

[0077]

[0078]

[0079] The results showed that the cell viability of the products prepared in experimental groups 1-4 was significantly higher than that of the products prepared in experimental groups 5-9, with the product prepared in experimental group 1 exhibiting the highest cell viability. This indicates that the products prepared in Examples 1, 2, 3, and 4 of this application possess a certain cell proliferation-promoting effect. Specifically, the synergistic effect of sea buckthorn extract, angelica extract, centella asiatica extract, peppermint extract, and ginger extract all have a significant impact on cell repair, and the product in Example 1 of experimental group 1 is the preferred product among them.

[0080] 2. In vitro antioxidant and anti-aging tests and results.

[0081] 2.1 DPPH free radical scavenging experiment.

[0082] Preparation of DPPH ethanol solution: Accurately weigh 4 mg of DPPH and place it in a 100 mL brown volumetric flask. Add 50 mL of ethanol, sonicate for 30 seconds, and dilute to the mark with ethanol. Shake well and set aside for use. This product must be prepared and used immediately.

[0083] Preparation of the test solution: the product of Example 1.

[0084] Operation steps: accurately pipette 2 mL of the test solution and 2 mL of DPPH solution and mix well; accurately pipette 2 mL of the test solution and 2 mL of ethanol and mix well; accurately pipette 2 mL of DPPH solution and 2 mL of ethanol and mix well, place at room temperature for 30 min, measure the absorbance at 515 nm wavelength, and calculate the free radical scavenging rate according to the following calculation formula:

[0085] IR% = [1-(Ai-Aj) / A0]*100%;

[0086] Wherein, Ai represents the absorbance of the solution after mixing the test solution and DPPH; Aj represents the absorbance of the solution after mixing the test solution and the solvent; A0 represents the absorbance of the solution after mixing DPPH and the solvent.

[0087] 2.2 ABTS + Free radical scavenging experiment.

[0088] Preparation of PBS buffer: weigh sodium chloride 8 g, potassium chloride 0.2 g, potassium dihydrogen phosphate 0.24 g, disodium hydrogen phosphate dodecahydrate 3.62 g, place in a 1000 mL beaker, add 800 mL distilled water, stir to dissolve, adjust the pH to 7.4 with hydrochloric acid or sodium hydroxide, transfer to a 1000 mL volumetric flask, dilute to the mark with distilled water, shake well, and use.

[0089] ABTS + Preparation of stock solution: accurately weigh ABTS + About 78 mg, place in a 20 mL brown volumetric flask, add 15 mL distilled water, ultrasonic for 5 min, dilute to the mark with distilled water, shake well. Accurately weigh potassium persulfate 76 mg or so, place in a 2 mL brown volumetric flask, add 1 mL distilled water, ultrasonic to dissolve, dilute to the mark with distilled water, shake well. Accurately pipette 352 μL of potassium persulfate solution into the ABTS solution, shake well, and stand overnight.

[0090] ABTS + Preparation of working solution: accurately pipette 1 mL of stock solution, add about 65 mL of PBS buffer, shake well. Preparation of test solution: weigh the external use of traditional Chinese medicine compound essential oil (Qiai essential oil 45 mL, mint essential oil 5 mL, camphor essential oil 15 mL, grape seed oil 10 mL, and honeysuckle essential oil 15 mL), place in a 250 mL conical flask, shake well, and obtain.

[0091] Operation steps: accurately take 0.5 mL of test solution and 5 mL of ABTS working solution and mix evenly; accurately take 0.5 mL of test solution and 5 mL of PBS buffer and mix evenly; accurately take 5 mL of ABTS working solution and 0.5 mL of PBS buffer and mix evenly, immediately measure the absorbance at 734 nm, and calculate the free radical scavenging rate according to the following formula:

[0092] IR% = [1-(Ai-Aj) / A0]*100%;

[0093] Wherein, Ai represents the absorbance of the solution after mixing the test solution and ABTS; Aj represents the absorbance of the solution after mixing the test solution and solvent; A0 represents the absorbance of the solution after mixing ABTS and solvent.

[0094] 2.3 SRSA superoxide anion free radical scavenging experiment.

[0095] Preparation of 0.1 moL / L PBS buffer (pH 7.4): weigh sodium chloride 80 g, potassium chloride 2 g, potassium dihydrogen phosphate 2.4 g, and dipotassium hydrogen phosphate trihydrate 23.1 g, and place them in a 1000 mL beaker. Add 600 mL of distilled water and stir until dissolved. Adjust the pH to 7.2 with hydrochloric acid or sodium hydroxide, and transfer to a 1000 mL volumetric flask. Dilute to the mark with distilled water, shake well, and use as needed.

[0096] Preparation of 150 μmoL / L NBT solution: accurately weigh NBT 12.5 mg and place it in a 100 mL brown volumetric flask. Add distilled water and sonicate until dissolved. Dilute to the mark with distilled water, shake well, and obtain.

[0097] Preparation of 60 μmoL / L PMS solution: accurately weigh PMS 18.8 mg and place it in a 1000 mL volumetric flask. Add distilled water and sonicate until dissolved. Dilute to the mark with distilled water, shake well, and obtain.

[0098] Preparation of 468 μmoL / L NADH solution: accurately weigh NADH 33.9 mg and place it in a 100 mL volumetric flask. Add distilled water and sonicate until dissolved. Dilute to the mark with distilled water, shake well, and obtain.

[0099] Preparation of test solution: weigh the external use of traditional Chinese medicine compound essential oil (Qiai essential oil 45 mL, mint essential oil 5 mL, camphor essential oil 15 mL, grape seed oil 10 mL, and verbena essential oil 15 mL), and place it in a conical flask. Shake well and obtain.

[0100] Preparation of working solution: take 1 mL 0.1 moL / L PBS buffer (pH 7.4) in a volumetric flask, add 1 mL 150 μmoL / L NBT solution, add 2 mL 468 μmoL / L NADH solution, add 1 mL 60 μmoL / L PMS solution, stir uniformly, react at 25℃ for 5 min, and measure the absorbance value at 560 nm wavelength.

[0101] Operation steps: accurately take 0.5 mL of the test sample solution and 5 mL of the above working solution and mix uniformly; accurately take 0.5 mL of the test sample solution and 5 mL of distilled water and mix uniformly; accurately take 5 mL of the above working solution and 0.5 mL of distilled water and mix uniformly, immediately measure the absorbance at 560 nm, and calculate the free radical scavenging rate according to the following formula:

[0102] IR% = [1-(Ai-Aj) / A0]*100%;

[0103] Wherein, Ai represents the absorbance of the solution after mixing the test solution and SRSA; Aj represents the absorbance of the solution after mixing the test solution and the solvent; A0 represents the absorbance of the solution after mixing SRSA and the solvent.

[0104] The test results are shown in Table 2:

[0105] Table 2 antioxidant activity analysis of test groups 1-4

[0106] Test sample DPPH scavenging rate (%) ABTS + % clearance SRSA scavenging rate (%) Test 1 group 97.1 92.6 86.3 Test 2 group 94.3 91.5 84.8 Test 3 group 92.1 91.1 84.4 Test 4 group 95.4 92.0 85.0

[0107] As shown in Table 2, the external use traditional Chinese medicine emulsion has good antioxidant and anti-aging activity. The product prepared in test group 1 has the best antioxidant and anti-aging activity, that is, the product of example 1 in test group 1 is the preferred product among the examples.

[0108] Application example two

[0109] Acid-dissolving and knot-dissolving test and results.

[0110] 1. Exercise endurance, lactic acid and muscle glycogen test of muscle tissue.

[0111] The experimental animals are purchased healthy male ICR mice 96, with animal feed and water provided to the mice free feeding, light and dark each 12 hours. After all the animals are adapted for one week, they are randomly divided into 12 groups, which are blank group, fatigue group, test 1-10 groups. The weight-bearing swimming modeling experiment is carried out for six weeks, and the body weight change of the mice is recorded every week. After six weeks of modeling, the test 1-10 groups start to give medicine, twice a day in the morning and evening, before giving medicine, the back of each group of mice is formed with uniform depilation area, the test 1-10 group medicine is applied to the area, and the medicine is given for three consecutive days. Then the determination of the exercise tolerance of all mice is carried out, and the behavior is tested by exhaustive swimming time: all mice are taken in the way of tail weight-bearing swimming, the tightness is appropriate, the total weight is 5% of the body weight, and the mice are individually placed in the swimming tank with water depth of 50 cm and water temperature of 25℃±1℃. From the time when the mice enter the water tank, when the mice swim to immerse in the water surface for more than 7s for 3 times in succession, it is considered as exhaustion, and the exhaustive swimming time is recorded. After the exercise tolerance of all mice is detected, the next day, all mice are swimming in the water tank without weight-bearing, and after swimming for 30 minutes, the mice are immediately killed by cervical dislocation method and dissected, the tissue samples (muscle) are divided and frozen in liquid nitrogen and transferred to-80℃ for frozen storage, for lactic acid and muscle glycogen analysis of muscle tissue, the determination of muscle lactic acid and muscle glycogen level is carried out according to the kit method. The results are shown in Table 3.

[0112] Table 3 Analysis of the effects of mouse exercise tolerance and muscle tissue lactic acid and muscle glycogen

[0113] Group Exhaustion swimming time (s) Muscle lactic acid (mmol / g) Muscle glycogen (mg / g) Blank group 827.32±294.46 0.058±0.003 0.854±0.095 Fatigue group 316.18±134.27 0.067±0.008 0.654±0.091 Test 1 group 919.22±218.51 0.049±0.004 0.824±0.122 Test 2 group 876.37±316.22 0.053±0.003 0.804±0.073 Test 3 group 891.41±296.59 0.050±0.007 0.808±0.088 Test 4 group 880.78±185.60 0.051±0.005 0.810±0.125 Test 5 group 641.59±368.36 0.062±0.003 0.713±0.804 Test 6 group 825.21±277.22 0.059±0.002 0.788±0.606 Test 7 group 805.11±178.86 0.058±0.004 0.767±0.817 Test 8 group 775.11±178.86 0.061±0.005 0.752±0.111 Test 9 group 416.25±308.16 0.065±0.008 0.680±0.106

[0114] The results show that, compared with the blank group, the weight-bearing swimming modeling experiment makes the exhaustive swimming time of the mice in the fatigue group significantly reduced, the muscle lactic acid of the mice is significantly increased, and the muscle glycogen level is significantly reduced; compared with the fatigue group, the products prepared in the test 1-8 groups can significantly increase the exhaustive swimming time, significantly reduce the muscle lactic acid, and significantly increase the muscle glycogen level, and the test 1-4 groups are stronger than the test 5-8 groups in the antagonism of exercise fatigue; and the test 9 group has no obvious improvement on the exhaustive swimming time, and has no significant effect on the fatigue indicators (muscle lactic acid and muscle glycogen) on the peripheral muscle tissue, which may be due to the lack of mint extract in the test 9 group, so that the effective components are difficult to penetrate the skin to the target site. In summary, Table 3 shows that the external use of traditional Chinese medicine emulsion (test 1-4 groups) of the application can significantly improve the anti-fatigue ability and exercise tolerance of mice, effectively reduce the accumulation of muscle lactic acid (acid excretion) and increase the energy reserve in the muscle in the process of maintaining the same exercise intensity, thereby effectively relieving exercise-induced fatigue. The acid excretion and anti-exercise fatigue effect of the product prepared in the test 1 group (Example 1) is the best.

[0115] 2. In-vivo blood stasis and knot-dissolving experiment and results.

[0116] 2.1 Effect on the blood rheology index of acute blood stasis type rats.

[0117] 100 rats were randomly divided into 10 groups, and administered once a day. Before administration, the back of each rat was shaved to form a uniform depilation area, and then the test drug was applied to the area. After 1 hour of the last administration, the rats were fixed, disinfected with 75% alcohol, and then placed in an ice bag for 1 hour. After rewarming in 45°C water for 5 minutes, the rats were normally fed for 3 days, and then blood was collected and the blood rheology index was measured. The results are shown in Table 4.

[0118] Table 4 Effect on the whole blood viscosity of rats

[0119]

[0120] 2.2 Effect on the adenosine diphosphate-induced platelet aggregation of rats.

[0121] 2 mL of blood was taken from the carotid artery, and anti-coagulated with 38 g / mL sodium citrate. After centrifugation at 100 r / min for 5 minutes at room temperature, platelet plasma (PRP) was obtained. Adenosine diphosphate was used as an inducer, and the platelet aggregation was measured by turbidity method on a SPA-III platelet aggregometer. The results were expressed as the maximum aggregation rate in 1 minute and the maximum aggregation rate in 5 minutes. The results are shown in Table 5.

[0122] Table 5 Effect of each group of rats on the adenosine diphosphate-induced platelet aggregation of rats

[0123]

[0124] The results of Tables 4 and 5 show that, compared with the blank control group or the group in which one of the traditional Chinese medicine extracts was omitted (test groups 5-9), the drug groups of the present application (test groups 1-4) had significant differences in the whole blood viscosity at high and low shear rates. In addition, compared with the blank control group or the group in which one of the traditional Chinese medicine extracts was omitted (test groups 5-9), the drug groups of the present application (test groups 1-4) had significant differences in the obvious inhibition of adenosine diphosphate-induced platelet aggregation of rats. The above results highlight the synergistic effect of sea buckthorn extract, angelica extract, centella asiatica extract, mint extract, and ginger extract in the common role of promoting blood circulation and removing blood stasis and resolving stagnation. The product prepared in test group 1 (Example 1) has the best effect of removing blood stasis and resolving stagnation.

[0125] Application of Example Three

[0126] In vivo anti-inflammatory and pain relief test and results

[0127] Test drug (invention group): 45 mL of Ai oil, 5 mL of mint oil, 15 mL of camphor oil, 10 mL of grape seed oil, and 15 mL of verbena oil. The mixture was placed in a conical flask and shaken to obtain the test drug.

[0128] Test animals: SD mice, provided by the Experimental Animal Center of Southern Medical University;

[0129] Statistical processing: the experimental results were expressed as and the significance of the difference between groups was determined by t-test.

[0130] 1. Anti-inflammatory test.

[0131] 1.1 Effect on mouse ear swelling: 60 mice were randomly divided into 6 groups, and administered once a day. Before administration, the back of each mouse was shaved to form a uniform area, and then the test drug was applied to the area. The administration was continued for 3 days, and 1 h after the last administration, 0.05 ml of xylene was applied to the right ear of the mouse. 2 h after the xylene was applied, the animals were sacrificed, the ears were cut, and the ear pieces were punched with an 8 mm steel punch. The weight difference between the left and right ears was taken as the degree of swelling, and the results are shown in Table 6.

[0132] Table 6 Effect on xylene-induced mouse ear swelling

[0133] Group Dose n Ear swelling degree (mg) Blank control group - 10 22.89±3.37 Aspirin group 0.234 g / kg 10 15.44±7.62 Test 1 group 0.1 mL 10 13.18±2.03 Test 2 group 0.1 mL 10 14.98±1.77 Test 3 group 0.1 mL 10 15.18±2.69 Test 4 group 0.1 mL 10 14.65±2.10

[0134] 1.2 Effect on mouse skin capillary permeability: 60 mice were randomly divided into 6 groups, and administered once a day. Before administration, the back of each mouse was shaved to form a uniform area, and then the test drug was applied to the area. The administration was continued for 3 days, and 1 h after the last administration, 1% Evans blue saline 0.1 ml / 10 g body weight was injected into the tail vein, and immediately 5 μg of histamine (0.1 ml) was injected intradermally into the abdomen. 20 min later, the animals were sacrificed, and the blue-dyed skin pieces were cut off and soaked in a mixture of acetone and saline (7:3). After 24 min, the supernatant was taken by centrifugation and colorimetric measurement was performed at 610 nm on a 721 spectrophotometer, and the results are shown in Table 7.

[0135] Table 7 Effect on mouse skin capillary permeability

[0136] Group Dose n Optical density (OD) of blue-stained skin Blank control group - 10 0.083±0.051 Aspirin group 0.234 g / kg 10 0.023±0.023 Test 1 group 0.1 mL 10 0.020±0.034 Test 2 group 0.1 mL 10 0.022±0.066 Test 3 group 0.1 mL 10 0.021±0.051 Test 4 group 0.1 mL 10 0.021±0.030

[0137] Note: compared with the blank group, ***P<0.01.

[0138] As shown by the anti-inflammatory results of Tables 6-7, compared with the blank group and the aspirin group, the drug groups (test groups 1-4) of the present application have significant differences in the effects on mouse ear swelling and the skin capillary permeability test. It can be seen that the external use of the traditional Chinese medicine compound essential oil of the present application (test groups 1-4) has good anti-inflammatory activity. The anti-inflammatory activity of the product prepared in test group 1 (Example 1) is the best and is superior to aspirin.

[0139] 2. Analgesic test.

[0140] 2.1 Effect on the acetic acid-induced writhing response in mice: 60 mice were taken and randomly divided into 6 groups. The mice were given the drug once a day. Before the administration, the back of each mouse was formed with a uniform depilation area. Then the test drug was applied to the area. The administration was continuously performed for 3 days. One hour after the last administration, 0.6% ice acetic acid 0.2 ml was injected into the abdominal cavity of each mouse. The writhing response (abdominal concave, stretched hind limbs, and raised hips) of each mouse within 20 minutes was observed and recorded. The results are shown in Table 8.

[0141] Table 8 Analgesic effect on the acetic acid-induced writhing response in mice

[0142] Group Dose n Twisting frequency (t = 20 min) Blank control - 10 28.53±3.46 Codeine group 0.008 g / kg 10 9.02±3.66 Test 1 group 0.1 mL 10 7.24±4.55 Test 2 group 0.1 mL 10 8.94±5.11 Test 3 group 0.1 mL 10 8.01±5.38 Test 4 group 0.1 mL 10 7.89±2.61

[0143] 2.2 Effect on the hot plate-induced licking foot pain threshold in mice: 20 mice were taken and randomly divided into 2 groups. The mice were given the drug once a day. Before the administration, the back of each mouse was formed with a uniform depilation area. Then the test drug was applied to the area. The administration was continuously performed for 3 days. One hour after the last administration, the pain threshold of each mouse was measured. The results are shown in Table 9.

[0144] Table 9 Effect on the hot plate-induced licking foot pain threshold in mice

[0145]

[0146]

[0147] The results of Tables 8-9 show that compared with the blank group and the codeine group, the drug groups (test groups 1-4) of the present application have significant differences in the acetic acid-induced writhing response in mice and the hot plate-induced licking foot pain threshold test. It can be seen that the external use of the traditional Chinese medicine compound essential oil of the present application has good analgesic activity. The analgesic activity of the product prepared in test group 1 (Example 1) is the best and is superior to codeine.

[0148] Application Example Four

[0149] Clinical acid-dissolving and knot-removing test and results.

[0150] Inventive group: product of Example 1, preferred group of the present invention;

[0151] Active oil group: zodiac active oil, 50 mL / bottle.

[0152] German horse paste group: KRAUTERHOF German horse paste, 500 g / bottle.

[0153] Anti-inflammatory analgesic patch group: Baiyunshan, 7 cm*10 cm*10 patches.

[0154] Sore muscle group: Sore muscle of Soto Pharmaceutical (soto), 30 g / branch.

[0155] As can be known by applying Examples 1, 2 and 3, the cell repair, anti-inflammatory analgesic and acid elimination and knot test results all outstandingly reflect the synergistic effect of sea buckthorn extract, angelica extract, gynura extract, mint extract and ginger extract, which have great advantages in jointly playing the role of promoting blood circulation and removing blood stasis, and the effect of the inventive group (Example 1) is the best. Therefore, next, we select the inventive group and the above-mentioned commonly seen products on the market at home and abroad which can eliminate inflammation and relieve pain symptoms in shoulder, neck, waist and limbs to compare the clinical effects of acid elimination and knot elimination.

[0156] 1. The test results of soreness and swelling of the shoulder, neck, waist and limbs caused by excessive lactic acid and lactic acid nodules in the body muscle.

[0157] 360 patients were selected as the object, and the shoulder, neck and waist congestion nodules were touched by hand or visible to the naked eye, and had obvious pain symptoms, and some patients could not sleep at night because of pain. These patients were treated as a course of 3 days, and were smeared three times a day at the position of pain and surrounding, and the propaganda and education was strengthened: adjust the mood, diet, work and rest, and avoid cold. The patients were evenly divided into 6 groups, and 60 patients in each group were further divided into three groups, namely patients with shoulder and neck pain, patients with waist pain and patients with limb pain, and the test results are shown in Table 10. Among them, the obvious improvement is that the pain of the pain site almost disappears, the improvement is that the pain of the pain site is slightly felt, no improvement is that the pain of the pain site is strong, and the repeated occurrence is that the pain of the pain site is strong after the obvious improvement or improvement after stopping the drug.

[0158]

[0159] Table 10 Test results of 1-8 groups on the clinical results of shoulder, neck and waist acid swelling pain caused by congestion nodules

[0160]

[0161]

[0162] As can be seen in Table 10, the number of people and the total effective rate of the inventive group are obviously higher than those of the active oil group, the German horse paste group, the anti-inflammatory analgesic patch group and the rubbing labor group; 98% of the patients with shoulder pain and neck pain in the inventive group are obviously improved and improved, and the recurrence rate is very low. In contrast, the number of recurrence of the active oil group, the German horse paste group, the anti-inflammatory analgesic patch group and the rubbing labor group is more than that of the inventive group, thereby reducing the total effective rate of the active oil group, the German horse paste group, the anti-inflammatory analgesic patch group and the rubbing labor group. This shows that the inventive group has a significant therapeutic effect on the shoulder, neck, waist and limb swelling caused by excessive lactic acid in the body muscle and lactic acid nodules, and is more targeted, and can play a role of "treating the symptoms and the root cause of the disease".

[0163] 2. Typical case analysis.

[0164] 2.1 Case one, Lei, male, 41 years old, Henan people. Shoulder and neck pain, neck belongs to obvious acid nodules, big vertebra position acid nodules, drum up, shoulder and neck pain for 1 year, often dizzy, paste a variety of ointment not good. Use the above inventive group product (example 1), external use, the second day shoulder and neck pain symptoms, every day in the position of pain and around three times, and simple massage, 1 day after the pain is eliminated, big vertebra drum place also smaller (such as Figure 1 ), continue to use 2 days consolidation, dizziness symptoms disappear, also easy to sleep, then 6 months no recurrence.

[0165] 2.2 Case two, Ye, male, 42 years old, Yangjiang, Guangdong, fireman, often after training calf pain, three days later can ease. Use the above inventive group product (example 1) external use, calf after training paste once, 15 minutes later, calf pain symptoms reduce 70%; continue 2 hours after the calf again paste the inventive group product, leg pain symptoms completely eliminated.

[0166] 2.3 Case three, Hu, male, 40 years old, Meizhou, Guangdong people, shoulder and neck pain, right shoulder belongs to obvious acid nodules, shoulder and neck pain for more than 1 year, and hand a little bit of numbness, difficult to sleep at night. Paste a variety of ointment and acupuncture, moxibustion, not good. Paste a variety of ointment before, a little bit of ease, not good, and repeatedly painful. Use the above inventive group product (example 1), external use, the second day shoulder and neck pain symptoms, every day in the position of pain and around three times, and simple massage, 1 day after the pain is eliminated, right shoulder nodules part presents dark red acid nodules dissipation symptoms (such as Figure 2 ), 3 days after the pain is eliminated, shoulder protruding part smaller and smaller, continue to use 3 days consolidation, then 1 year no recurrence.

[0167] Therefore, the external-use traditional Chinese medicine emulsion for relieving acid and removing obstruction of the present application is suitable for treating the shoulder, neck, waist and limb swelling and pain caused by excessive muscle lactic acid and lactic acid nodules, wherein the synergistic effect of the five traditional Chinese medicines, i.e., sea buckthorn extract, angelica extract, centella asiatica extract, mint extract and ginger extract, has a remarkable therapeutic effect on the shoulder, neck, waist and limb swelling and pain caused by excessive muscle lactic acid and lactic acid nodules and is more targeted, and can play a role of treating both symptoms and root causes and seeking root causes to treat diseases. In addition, the product of the present application directly acts on the skin, is quickly absorbed by the body, is convenient to apply, and has a pleasant taste.

[0168] The technical features of the above-described embodiments can be combined in any manner. In order to make the description simple, all possible combinations of the technical features in the above-described embodiments are not described, but as long as the combinations of the technical features do not contradict each other, they should be considered as falling within the scope of the present disclosure.

[0169] The above-described embodiments only express several implementation manners of the present application, and the description is relatively specific and detailed, but should not be understood as a limitation on the scope of the patent. It should be pointed out that, for those skilled in the art, several modifications and improvements can be made without departing from the concept of the present application, and these all fall within the protection scope of the present application. Therefore, the protection scope of the patent of the present application should be subject to the appended claims.

Claims

1. A traditional Chinese medicine emulsion for relieving acid reflux, characterized in that, The pharmaceutical active ingredient and the pharmaceutically acceptable excipient have a weight ratio of (0.8-13):(13-60.5); the pharmaceutical active ingredient comprises raw materials in a weight ratio of 2. The traditional Chinese medicine emulsion according to claim 1, characterized in that, The pharmaceutical active ingredient comprises raw materials in a weight ratio of 3. The traditional Chinese medicine emulsion according to claim 1, characterized in that, The excipient comprises raw materials in a weight ratio of 4. The traditional Chinese medicine emulsion according to claim 3, characterized in that, The excipient comprises raw materials in a weight ratio of 5. The use of the traditional Chinese medicine emulsion in any one of claims 1-4 in the preparation of a medicine for expelling acid and resolving stasis.

6. The use of the traditional Chinese medicine emulsion in any one of claims 1-4 in the preparation of a medicine for relieving pain and / or swelling.

7. Use according to claim 6, characterized in that, The medicine for relieving pain and / or swelling is a medicine for relieving or eliminating pain and / or swelling caused by muscle lactic acid and lactic acid nodules.

8. A medicament for draining and unblocking, characterized by, The effective component comprises the traditional Chinese medicine emulsion in any one of claims 1-4.

9. A medicament for analgesia and / or decongestion, characterized in that, The effective component comprises the traditional Chinese medicine emulsion in any one of claims 1-4.

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