Biopsy sample flattening, fixing and embedding box and use method thereof
By designing a biopsy sample flattening, fixation, and embedding cassette, the problems of missed diagnoses, misdiagnoses, and cross-contamination caused by non-standard operation in existing technologies have been solved, achieving standardized fixation of biopsy samples and improving the accuracy of pathological diagnosis.
Patent Information
- Application Number
- CN202411626071.2
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-11-14
- Publication Date
- 2026-01-23
AI Technical Summary
The lack of dedicated biopsy sample storage tools in existing technologies leads to non-standard operation, difficulty in fixation, insufficient tissue mounting, easy missed or misdiagnosis, and easy damage or loss during the sampling process, posing a risk of cross-contamination.
A biopsy sample flattening and fixation embedding cassette is designed, comprising an embedding cassette, a cassette body, an adhesive fixation layer, and a sliding plate. Through the design of scale markings and friction force, the biopsy sample is standardized and fixed, avoiding tissue damage and cross-contamination.
It enables standardized storage and fixation of biopsy samples, reduces the risk of missed or misdiagnosed cases, improves the accuracy of pathological diagnosis, simplifies the operation process, and avoids tissue damage and cross-contamination.
Smart Images
Figure CN121369359A_ABST
Abstract
Description
TECHNICAL FIELD
[0001] The present application belongs to the technical field of medical devices, and particularly relates to a biopsy sample flattening, fixing and embedding box and a use method thereof. BACKGROUND
[0002] Currently, after a gastrointestinal mirror biopsy takes a tissue biopsy sample, there is no special biopsy storage (fixing) paper, and usually, an operator uses discarded daily copy paper, advertising paper and the like to manually cut small paper strips or small paper blocks for use, which is not standardized and can easily lead to the following disadvantages: 1) The temporary paper strips have different materials, and the size, thickness, surface friction and mucus permeability of the paper strips have no unified standards, so that it is not easy to operate when the biopsy sample is fixed, the force is not easy to control, the mucosa surface or the largest surface of the tissue is not fully exposed, and time and effort are wasted; 2) The tissue is curled when it is fixed, and it is not easy to unfold, so that the layers of the mucosa tissue cannot be fully exposed when it is fixed, which causes difficulties in pathological diagnosis (especially pathological diagnosis of gastrointestinal mucosa heterotypic hyperplasia and early gastrointestinal cancer), and misdiagnosis and missed diagnosis are easy to occur; 3) The biopsy forceps are overused during the flattening process of the biopsy sample, so that the tissue is physically damaged, and the pathological histological observation is affected; 4) Improper operation causes the tissue to fall off or be lost, or the tissue is not easy to peel off from the paper strip when pathological material is taken, and the tissue is physically damaged and broken; 5) The biopsy sample after flattening and fixing needs to be transferred for subsequent operation, on the one hand, when the volume of the biopsy sample is small, the biopsy sample needs to be carefully searched when it is taken, and since the fixed tissue is fragile, it is easy to be lost by using forceps to clamp, time and effort are wasted, and inconvenience is caused for subsequent operation; on the other hand, during the transfer process of the biopsy sample, the biopsy sample is peeled off from the paper strip by using forceps, and the residue of the biopsy sample easily pollutes the forceps, and the next biopsy sample is at risk of being polluted when the next biopsy sample is peeled off by using the forceps, which finally causes misdiagnosis. SUMMARY
[0003] The main purpose of the present application is to overcome the deficiencies in the prior art, to solve the technical problems of standard storage, fixation and embedding of biopsy samples, and to provide a biopsy sample flattening, fixing and embedding box and a use method thereof.
[0004] The present application is implemented by the following technical scheme: a biopsy sample flattening, fixing and embedding box, which comprises an embedding box, a box body, a pasting fixing layer, a sliding plate and a flattening fixing plate, the pasting fixing layer comprises a first pasting fixing layer and a second pasting fixing layer. The middle part of the inner cavity of the embedding box is provided with a plurality of positioning columns, and the box body is detachably clamped between the positioning columns; an observation window is arranged at the edge position of the upper cover of the embedding box, a first scale for marking the width of the biopsy sample is arranged at the upper side edge position of the observation window, and a second scale for marking the length of the biopsy sample is arranged at the left side or right side edge position of the observation window, and the first scale and the second scale are perpendicular to each other; The box body includes a hollow inner cavity, the tail part of the inner cavity of the box body is communicated with the inner cavity of the embedding box, the first end of the top surface of the box body is provided with a through hole, the through hole is communicated with the inner cavity, and the upper part of the through hole is covered with a guide plate, and the top surface and the guide plate are made of hard transparent material. The flattened fixing plate is detachably inserted on the bottom surface of the inner cavity of the box body, the tail part of the flattened fixing plate penetrates through the box body and extends to the outside of the embedding box, and the first adhesive fixing layer is fixedly installed at the first end of the flattened fixing plate in the inner cavity of the box body; the sliding plate is arranged above the flattened fixing plate, a gap is arranged between the sliding plate and the top surface, the tail part of the sliding plate penetrates through the box body and extends to the outside of the embedding box, the sliding plate slides along the length direction of the inner cavity of the box body, and the second adhesive fixing layer is fixedly installed at the first end of the sliding plate in the inner cavity of the box body; after the upper cover of the embedding box is opened, the biopsy sample to be flattened is put into the box body through the through hole, one end of the biopsy sample to be flattened is adhered to the first adhesive fixing layer, and the other end of the biopsy sample to be flattened is adhered to the second adhesive fixing layer; after the upper and lower surfaces of the embedding box are closed, the sliding plate slides to the outside of the embedding box and drives the biopsy sample to be flattened on the flattened fixing plate, then the flattened fixing plate slides to the outside of the embedding box, until the flattened biopsy sample is located directly below the observation window, and then subsequent embedding operation of the embedding box is performed.
[0005] Further, the front and rear side walls of the inner cavity of the box body are symmetrically provided with sliding grooves along the length direction of the box body, and the edges of the sliding plate are installed in the corresponding sliding grooves.
[0006] Further, the first end of the sliding groove in the inner cavity is provided with a limiting block, the limiting block limits the sliding plate to slide to the stroke termination position along the sliding groove, in this state, the first adhesive fixing layer and the second adhesive fixing layer are located directly below the through hole, and the first adhesive fixing layer and the second adhesive fixing layer are arranged in a staggered manner.
[0007] Further, the inner wall of the guide plate is provided as a spherical surface or an ellipsoidal surface.
[0008] Further, the friction between the bottom surface of the box body and the flattened fixing plate is greater than the friction between the sliding plate and the flattened fixing plate.
[0009] Further, the middle part of the upper surface of the flattened fixing plate is provided with a groove along the length direction, the groove contains pathological material special ink dye, and the lower surface of the flattened biopsy sample is colored by the pathological material special ink dye after the biopsy sample is flattened.
[0010] A method for using a biopsy sample flattening, fixation, and embedding cassette as described above includes the following steps: S1. First, the first adhesive fixing layer is fixedly installed at the first end of the flattening fixing plate, and then the flattening fixing plate is inserted into the inner cavity of the box body; then, the second adhesive fixing layer is fixedly installed at the first end of the sliding plate, and then the sliding plate is inserted into the inner cavity of the box body until the end of the sliding plate approaches the first adhesive fixing layer. S2. Use biopsy forceps to take the biopsy sample to be flattened and put it into the through hole of the box body through the through hole. One end of the biopsy sample to be flattened is pasted on the first adhesive fixing layer and the other end is pasted on the second adhesive fixing layer. S3. Place the box and biopsy sample prepared in step S2 into the embedding cassette, so that the box is locked between the positioning columns, and then close the embedding cassette. S4. First, manually drive the sliding plate to slide outwards from the embedding cassette. During the sliding process, the biopsy sample enters the gap between the sliding plate and the top surface through the guide plate until the biopsy sample is flattened on the flattening fixing plate. Then, manually drive the flattening fixing plate to slide outwards from the embedding cassette until the flattened biopsy sample is directly below the observation window. Observe and fine-tune the relative position of the sliding plate and the flattening fixing plate until the biopsy sample is completely flattened. Finally, mark the dimensions of the flattened biopsy sample using the first and second scales. S5. Place the embedding box into the fixative for subsequent operations.
[0011] The beneficial effects of this invention are as follows: 1) The operation is simple, time-saving, labor-saving and standardized, and the biopsy sample collection process is optimized. The biopsy sample can be flattened and fixed in the embedding box, which facilitates subsequent operations and can avoid cross-contamination that is easy to occur during tissue peeling and transfer. 2) Fully extend small tissues: The size of biopsy samples is usually 1-5mm. Due to the force of clamping and the tissue characteristics of the digestive tract mucosa, the biopsy samples tend to curl up during fixation. This invention can save time and effort, while ensuring that the biopsy sample is fully exposed on the mucosal surface, lamina propria and muscularis mucosae during fixation, so as to meet the requirements of pathological sampling and diagnosis and avoid missed diagnosis and misdiagnosis. 3) Fixing rough areas during mounting prevents biopsy samples from becoming difficult to obtain pathological samples due to mounting on the paper edge, and avoids them falling off into the fixative during transportation; 4) Setting rulers along the length and width directions at the observation window position as reference objects can reduce the workload of pathologists in measuring with a ruler when collecting samples; 5) The grooves on the upper surface of the flattening and fixing plate contain special ink dye for pathological sampling, which stains the lower surface of the biopsy sample while it is flattened and fixed, thus helping pathologists to more easily identify the embedding surface when embedding tissue. Attached Figure Description
[0012] Figure 1 This is a top view of the structure of the present invention in the initial state of a biopsy sample (the top cover of the embedding cassette is omitted). Figure 2 for Figure 1 Enlarged front view sectional view of the middle box structure; Figure 3 for Figure 2 Top view of the middle box structure; Figure 4 This is a schematic diagram of the magnified front view and cross-sectional structure of the box during the flattening process of the biopsy sample. Figure 5 A top view of the structure of this invention with the biopsy sample flattened out; Figure 6 for Figure 5 The diagram shows the top view of the invention after omitting the top cover of the embedding box.
[0013] In the figure, 1 is the box body, 2 is the top surface, 3 is the guide plate, 4 is the through hole, 5 is the biopsy sample, 6 is the first adhesive fixing layer, 7 is the second adhesive fixing layer, 8 is the sliding plate, 9 is the first scale, 10 is the second scale, 11 is the flattening fixing plate, 12 is the positioning post, 13 is the embedding box, and 14 is the observation window. Detailed Implementation
[0014] The present invention will now be described in further detail with reference to the accompanying drawings and embodiments.
[0015] like Figures 1 to 6 The biopsy sample flattening and fixation embedding cassette shown includes an embedding cassette 13, a cassette body 1, an adhesive fixation layer, a sliding plate 8, and a flattening fixation plate 11. The adhesive fixation layer includes a first adhesive fixation layer 6 and a second adhesive fixation layer 7. The embedding box 13 has several positioning posts 12 in the middle of its inner cavity, and the box body 1 is detachably snapped between the positioning posts 12; an observation window 14 is provided at the edge of the top cover of the embedding box 13, a first scale 9 for marking the width of the biopsy sample 5 is provided at the upper edge of the observation window 14, and a second scale 10 for marking the length of the biopsy sample 5 is provided at the left or right edge of the observation window 14, and the first scale 9 and the second scale 10 are perpendicular to each other; The box body 1 includes a hollow inner cavity. The tail end of the inner cavity of the box body 1 is connected to the inner cavity of the embedding box 13. A through hole 4 is provided at the first end of the top surface 2 of the box body 1. The through hole 4 is connected to the inner cavity. A guide plate 3 is covered above the through hole 4. Both the top surface 2 and the guide plate 3 are made of rigid transparent material. The inner wall of the guide plate 3 is set as a spherical or ellipsoidal surface, which serves as a guide while avoiding scratching the tissue. The flattening fixing plate 11 is detachably inserted into the bottom surface of the inner cavity of the box body 1. The tail of the flattening fixing plate 11 penetrates the box body 1 and extends to the outside of the embedding box 13. The first adhesive fixing layer 6 is fixedly installed at the head end of the flattening fixing plate 11 located in the inner cavity of the box body 1. The sliding plate 8 is placed above the flattening fixing plate 11. A gap is provided between the sliding plate 8 and the top surface 2. The tail of the sliding plate 8 penetrates the box body 1 and extends to the outside of the embedding box 13. The sliding plate 8 slides along the length direction of the inner cavity of the box body 1. The second adhesive fixing layer 7 is fixedly installed at the head end of the sliding plate 8 located in the inner cavity of the box body 1. After opening the top cover of the embedding cassette 13, the biopsy sample 5 to be flattened is placed into the cassette 1 through the through hole 4. One end of the biopsy sample 5 to be flattened is attached to the first adhesive fixing layer 6, and the other end of the biopsy sample 5 to be flattened is attached to the second adhesive fixing layer 7. After closing the upper and lower surfaces of the embedding cassette 13, the sliding plate 8 slides to the outside of the embedding cassette 13, thereby causing the biopsy sample 5 to be flattened on the flattening fixing plate 11. Then the flattening fixing plate 11 slides to the outside of the embedding cassette 13 until the flattened biopsy sample 5 is located directly below the observation window 14, and then the subsequent embedding operation of the embedding cassette 13 is performed.
[0016] Furthermore, symmetrical grooves are provided on the front and rear side walls of the inner cavity of the box 1 along the length direction of the box 1, and the edge of the sliding plate 8 is installed in the corresponding groove, so that the sliding plate 8 is more stable and will not float or warp locally during the sliding process, thus avoiding wear on the surface of the biopsy sample 5.
[0017] Furthermore, a limiting block is provided at the beginning of the sliding groove in the inner cavity. The limiting block restricts the sliding plate 8 from sliding along the sliding groove to the end position of the stroke. In this state, the first adhesive fixing layer 6 and the second adhesive fixing layer 7 are both located directly below the through hole 4, and the first adhesive fixing layer 6 and the second adhesive fixing layer 7 are staggered.
[0018] Furthermore, the friction between the bottom surface of the box 1 and the flattening fixing plate 11 is greater than the friction between the sliding plate 8 and the flattening fixing plate 11. On this basis, a groove is provided in the middle of the upper surface of the flattening fixing plate 11 along the length direction. The groove contains a special ink dye for pathological sampling. When the biopsy sample 5 is flattened, the lower surface is just colored by the special ink dye for pathological sampling.
[0019] A method for using a biopsy sample flattening, fixation, and embedding cassette as described above includes the following steps: S1. First, the first adhesive fixing layer 6 is fixedly installed at the first end of the flattening fixing plate 11, and then the flattening fixing plate 11 is inserted into the inner cavity of the box body 1; then, the second adhesive fixing layer 7 is fixedly installed at the first end of the sliding plate 8, and then the sliding plate 8 is inserted into the inner cavity of the box body 1 until the end of the sliding plate 8 approaches the first adhesive fixing layer 6. S2. Use biopsy forceps to take the biopsy sample 5 to be flattened and put it into the through hole 4 of the box body 1 through the through hole 4. One end of the biopsy sample 5 to be flattened is pasted on the first adhesive fixing layer 6 and the other end is pasted on the second adhesive fixing layer 7. S3. Place the box 1 and biopsy sample 5 prepared in step S2 into the embedding cassette 13, so that the box 1 is locked between the positioning posts 12, and then close the embedding cassette 13. S4. First, manually drive the sliding plate 8 to slide outwards from the embedding cassette 13. During the sliding process, the biopsy sample 5 enters the gap between the sliding plate 8 and the top surface 2 through the guide plate 3 until the biopsy sample 5 is flattened on the flattening fixing plate 11. Then, manually drive the flattening fixing plate 11 to slide outwards from the embedding cassette 13 until the flattened biopsy sample 5 is directly below the observation window 14. Observe and fine-tune the relative position of the sliding plate 8 and the flattening fixing plate 11 until the biopsy sample 5 is completely flattened. Finally, mark the dimensions of the flattened biopsy sample 5 using the first scale 9 and the second scale 10. S5. Place the embedding box 13 into the fixative for subsequent operations.
[0020] The above description is merely a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in the present invention should be included within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.
Claims
1. A biopsy sample flattening, fixation, and embedding cassette, characterized in that: It includes an embedding box (13), a box body (1), an adhesive fixing layer, a sliding plate (8) and a flat fixing plate (11), and the adhesive fixing layer includes a first adhesive fixing layer (6) and a second adhesive fixing layer (7); The embedding box (13) has several positioning posts (12) in the middle of its inner cavity, and the box body (1) is detachably clamped between the positioning posts (12); an observation window (14) is provided at the edge of the top cover of the embedding box (13), a first scale (9) for marking the width of the biopsy sample (5) is provided at the upper edge of the observation window (14), and a second scale (10) for marking the length of the biopsy sample (5) is provided at the left or right edge of the observation window (14), and the first scale (9) and the second scale (10) are perpendicular to each other; The box body (1) includes a hollow inner cavity. The tail end of the inner cavity of the box body (1) is connected to the inner cavity of the embedding box (13). A through hole (4) is provided at the first end of the top surface (2) of the box body (1). The through hole (4) is connected to the inner cavity. A guide plate (3) is provided above the through hole (4). The top surface (2) and the guide plate (3) are both made of hard transparent material. The flattening fixing plate (11) is detachably inserted into the bottom surface of the inner cavity of the box body (1). The tail of the flattening fixing plate (11) penetrates the box body (1) and extends to the outside of the embedding box (13). The first adhesive fixing layer (6) is fixedly installed at the head end of the flattening fixing plate (11) located in the inner cavity of the box body (1). The sliding plate (8) is placed above the flattening fixing plate (11). A gap is provided between the sliding plate (8) and the top surface (2). The tail of the sliding plate (8) penetrates the box body (1) and extends to the outside of the embedding box (13). The sliding plate (8) slides along the length direction of the inner cavity of the box body (1). The second adhesive fixing layer (7) is fixedly installed at the head end of the sliding plate (8) located in the inner cavity of the box body (1). After opening the top cover of the embedding box (13), the biopsy sample (5) to be flattened is placed into the box body (1) through the through hole (4). One end of the biopsy sample (5) to be flattened is pasted on the first adhesive fixing layer (6), and the other end of the biopsy sample (5) to be flattened is pasted on the second adhesive fixing layer (7). After closing the upper and lower surfaces of the embedding box (13), the sliding plate (8) slides to the outside of the embedding box (13) and drives the biopsy sample (5) to be flattened on the flattening fixing plate (11). Then the flattening fixing plate (11) slides to the outside of the embedding box (13) until the flattened biopsy sample (5) is located directly below the observation window (14), and then the subsequent embedding operation of the embedding box (13) is carried out.
2. The biopsy sample flattening, fixation, and embedding cassette according to claim 1, characterized in that: The front and rear sidewalls of the inner cavity of the box (1) are symmetrically provided with sliding grooves along the length direction of the box (1), and the edge of the sliding plate (8) is installed in the corresponding sliding groove.
3. The biopsy sample flattening, fixation, and embedding cassette according to claim 2, characterized in that: A limiting block is provided at the beginning of the slide groove in the inner cavity. The limiting block restricts the sliding plate (8) from sliding along the slide groove to the end of the stroke position. In this state, the first adhesive fixing layer (6) and the second adhesive fixing layer (7) are both located directly below the through hole (4), and the first adhesive fixing layer (6) and the second adhesive fixing layer (7) are misaligned.
4. The biopsy sample flattening, fixation, and embedding cassette according to claim 1, characterized in that: The inner wall of the guide plate (3) is set as a spherical or ellipsoidal surface.
5. The biopsy sample flattening, fixation, and embedding cassette according to claim 1, characterized in that: The friction between the bottom surface of the box (1) and the flattening fixing plate (11) is greater than the friction between the sliding plate (8) and the flattening fixing plate (11).
6. The biopsy sample flattening, fixation, and embedding cassette according to claim 1, characterized in that: The flattening fixing plate (11) has a groove in the middle of its upper surface along the length direction. The groove contains a special ink dye for pathological sampling. When the biopsy sample (5) is flattened, the lower surface is just colored by the special ink dye for pathological sampling.
7. A method of using the biopsy sample flattening, fixation, and embedding cassette as described in claim 1, characterized in that, Includes the following steps: S1. First, the first adhesive fixing layer (6) is fixedly installed at the first end of the flattening fixing plate (11), and then the flattening fixing plate (11) is inserted into the inner cavity of the box body (1); then, the second adhesive fixing layer (7) is fixedly installed at the first end of the sliding plate (8), and then the sliding plate (8) is inserted into the inner cavity of the box body (1) until the end of the sliding plate (8) approaches the first adhesive fixing layer (6). S2. Use biopsy forceps to take the biopsy sample (5) to be flattened and put it into the through hole (4) of the box body (1) through the through hole (4). One end of the biopsy sample (5) to be flattened is pasted on the first adhesive fixing layer (6), and the other end is pasted on the second adhesive fixing layer (7). S3. Place the box (1) prepared in step S2 and the biopsy sample (5) into the embedding box (13), so that the box (1) is locked between the positioning posts (12), and then close the embedding box (13). S4. First, manually drive the sliding plate (8) to slide out of the embedding cassette (13). During the sliding process, the biopsy sample (5) enters the gap between the sliding plate (8) and the top surface (2) through the guide plate (3) until the biopsy sample (5) is flattened on the flattening fixing plate (11). Then, manually drive the flattening fixing plate (11) to slide out of the embedding cassette (13) until the flattened biopsy sample (5) is directly below the observation window (14). Observe and fine-tune the relative position of the sliding plate (8) and the flattening fixing plate (11) until the biopsy sample (5) is completely flattened. Finally, mark the size of the flattened biopsy sample (5) with the first scale (9) and the second scale (10). S5. Place the embedding box (13) into the fixative for subsequent operations.