Anti-hair-loss and hair-strengthening scalp care essence

Abstract

The invention provides anti-alopecia hair-strengthening scalp care essence and a preparation method thereof. The anti-alopecia hair-strengthening scalp care essence is prepared from the following raw materials in parts by mass: 50 parts of water; 8-12 parts of a humectant; 1-3 parts of a cosolvent; 0.3 to 0.6 part of organic acid; 0.5-1 part of a preservative; 2-5 parts of a plant extract; 3.5 to 4.5 parts of a nicotinamide-citryl flavone bisamide derivative; 15 to 20 parts of a signal peptide-zinc gluconate complex; 25 to 35 parts of a citryl amino acid amide derivative; 0.3 to 0.6 part of caffeine; 1-2 parts of a yeast extract; and 0.1-0.3 part of costus oil. The anti-alopecia hair-strengthening scalp care essence can prevent alopecia and improve the hair density.

Description

Technical Field

[0001] This application relates to the field of daily cosmetics technology, specifically to a hair loss prevention and hair strengthening scalp care essence. Background Technology

[0002] In modern life, hair loss is becoming increasingly common due to multiple factors. Most existing hair loss prevention and hair-strengthening products focus on cleansing and oil control, nutritional supplementation, or hormone regulation. While these can improve the scalp environment or slow down the hair loss process to some extent, they generally have the following problems: The single active ingredient is compounded with poor stability, and the permeability and bioavailability of the active ingredients are insufficient, making it difficult to achieve synergistic regulation of multiple pathways at the same time and to effectively act on the deep layers of hair follicles in the scalp. Without systematic repair and activation of the hair follicle microenvironment, it is difficult to improve the hair ecology from the root cause; Therefore, there is an urgent need to develop a non-single compound scalp care essence with multiple active ingredients, high stability, and a clear synergistic mechanism to meet consumers' needs for preventing hair loss and increasing hair density. Summary of the Invention

[0003] This invention provides a hair loss prevention and hair strengthening scalp care essence and its preparation method.

[0004] In one aspect, this application provides an anti-hair loss and hair-strengthening scalp care essence, comprising, by weight, the following ingredients: 50 parts water; 8-12 parts moisturizer; 1-3 parts solubilizer; 0.3-0.6 parts organic acid; 0.5-1 part preservative; 2-5 parts plant extract; 3.5-4.5 parts niacinamide-citryl flavonoid diamide derivative; 15-20 parts signal peptide-zinc gluconate complex; 25-35 parts citryl amino acid amide derivative; 0.3-0.6 parts caffeine; 1-2 parts yeast extract; and 0.1-0.3 parts costus root oil. This anti-hair loss and hair-strengthening scalp care essence can prevent hair loss and increase hair density.

[0005] Through the above-described embodiments, the nicotinamide-citryl flavonoid diamide derivative and caffeine work synergistically to regulate sebum secretion, promote scalp microcirculation, and inhibit 5α-reductase activity, thereby improving the hair follicle microenvironment, delaying hair follicle atrophy, prolonging the hair growth cycle, constructing a multi-target anti-hair loss mechanism, and increasing hair density and growth activity. The signal peptide-zinc gluconate complex and yeast extract together provide hair follicles with nutrients such as zinc, amino acids, and B vitamins, activating hair follicle metabolism, promoting keratin synthesis, and enhancing hair root stability and hair strand toughness. The citryl amino acid amide derivative and moisturizer synergistically construct a scalp moisturizing barrier. This product contains ingredients that mimic the structure of natural moisturizing factors, enhancing the tolerance of the stratum corneum and relieving discomfort such as dryness and itching. Plant extracts, rich in various active ingredients, offer multiple benefits including antioxidant, anti-inflammatory, and blood circulation-promoting effects, further enhancing the anti-hair loss and hair-strengthening scalp care essence. Organic acids adjust the pH of the essence to a slightly acidic range, maintaining scalp environment stability and microecological balance. Preservatives and wood oil work together to ensure the product's microbial safety and gentleness, avoiding irritation and making it suitable for long-term use. Wood oil also imparts a natural fragrance and soothing experience, enhancing sensory pleasure during use.

[0006] In some embodiments, the anti-hair loss and hair-strengthening scalp care serum meets at least one of the following conditions: 1) The moisturizer includes at least one of butylene glycol, 1,2-pentanediol, propylene glycol, 1,2-hexanediol, and octyl glycol; 2) The cosolvent includes at least one of disodium EDTA, PEG-6 octanoic acid / capric acid glycerides, and PEG-40 hydrogenated castor oil; 3) The organic acid includes at least one of citric acid and lactic acid; 4) The preservative includes at least one of phenoxyethanol and p-hydroxyacetophenone; 5) The plant extracts include at least one of the following: Platycladus orientalis leaf extract, Anemarrhena asphodeloides root extract, Ginseng root extract, Ginger root extract, Angelica sinensis root extract, Polygonum multiflorum root extract, and Horse chestnut seed extract.

[0007] Through the above embodiments, butylene glycol, 1,2-pentanediol, propylene glycol, 1,2-hexanediol, and caprylyl glycol are all low-irritant polyol moisturizers that can form a hydrophilic moisturizing film on the scalp surface, maintain the moisture content of the stratum corneum, relieve dryness, tightness, and other discomforts, and enhance the scalp barrier function; the disodium EDTA in the solubilizer can chelate metal ions, stabilizing the formulation system of the anti-hair loss and hair-strengthening scalp care essence; PEG-6 caprylic / capric glycerides, PEG-40... Hydrogenated castor oil enhances the solubility and permeability of fat-soluble active ingredients, thereby improving the stability and bioavailability of various functional components in the anti-hair loss and hair-strengthening scalp care essence. Organic acids such as citric acid and lactic acid adjust the pH of the system to a suitable slightly acidic range for the scalp, maintaining the scalp's microecological balance and improving the product's gentleness and skin compatibility. Preservatives such as phenoxyethanol and p-hydroxyacetophenone have broad-spectrum antibacterial capabilities, achieving good microbial control even at low concentrations, ensuring product safety. The plant extracts are rich in various plant active ingredients, possessing multiple effects such as anti-oxidation, anti-inflammation, promoting blood circulation, and nourishing hair follicles, synergistically enhancing the anti-hair loss and hair-strengthening effect of the anti-hair loss and hair-strengthening scalp care essence.

[0008] In some embodiments, the nicotinamide-citryl flavonoid diamide derivative includes the following preparation steps: M1: Flavonoids, citric acid, and Novozymes lipase 435 are dispersed in isopropanol, and the flavonoids and citric acid undergo an amidation reaction to obtain a dispersion of carboxylated flavonoid derivatives. M2: The dispersion of the carboxylated flavonoid derivative is mixed with nicotinamide to induce an amidation reaction between the carboxylated flavonoid derivative and nicotinamide, thereby obtaining a nicotinamide-citryl flavonoid diamide derivative. The flavonoids include at least one of rutin, quercetin, naringin, and 5,7-dihydroxyflavone.

[0009] Through the above embodiments, the preparation method of the nicotinamide-citryl flavonoid diamide derivative is based on the amidation modification of flavonoid compounds and citric acid, further introducing nicotinamide structural units to construct a functional molecular skeleton with diamide bond linkages, possessing both the antioxidant and anti-inflammatory properties of flavonoids and the skin conditioning and barrier repair capabilities of nicotinamide; among which, flavonoid compounds such as rutin, quercetin, naringin, and 5,7-dihydroxyflavone are all natural polyphenolic substances with multiple physiological activities such as scavenging free radicals, inhibiting the expression of inflammatory factors, and promoting microcirculation, which can effectively improve the scalp microenvironment and relieve inflammation and oxidative stress; the introduction of citric acid not only Providing carboxyl sites for subsequent amidation reactions also enhances the water solubility and reactivity of flavonoids. Novozymes lipase 435, as a green catalyst, offers high selectivity and mild reaction conditions, avoiding side reactions and harmful residues associated with traditional chemical catalysts, thus improving product purity and biosafety. Subsequent amidation with nicotinamide further enhances molecular polarity and hydrophilicity, improving its dispersion stability and bioavailability in aqueous systems. The resulting nicotinamide-citryl flavonoid diamide derivative can exert multiple effects in scalp care products, including anti-inflammatory, antioxidant, sebum-regulating, blood circulation-promoting, and hair follicle-activating properties.

[0010] Furthermore, the nicotinamide-citryl flavonoid diamide derivative comprises the following preparation steps: M1: Disperse 100 parts of flavonoids, 10-30 parts of citric acid, and 5-10 parts of Novozymes lipase 435 in 500-800 parts of isopropanol, and stir at 300-500 rpm for 4-8 hours at 40-60℃ to obtain a dispersion of carboxylated flavonoid derivatives. M2: Mix the dispersion of the carboxylated flavonoid derivative with 80-120 parts of nicotinamide and stir at 400-600 rpm for 8-12 h at 40-60℃ to obtain nicotinamide-citryl flavonoid diamide derivative.

[0011] Through the above-described embodiments, flavonoids and citric acid can undergo amidation under the above reaction conditions with the help of the highly selective catalysis of Novozymes lipase 435, precisely introducing a carboxyl group structure, avoiding side reactions, and improving the yield and purity of the obtained carboxylated flavonoid derivatives. Subsequently, the carboxylated flavonoid derivatives are reacted with nicotinamide under appropriate conditions to further complete the construction of the amide bond between the carboxyl and amino groups, forming a structurally stable diamide linkage framework, enhancing the molecular integrity and functional synergy of the product nicotinamide-citryl flavonoid diamide derivative. The controlled amount of isopropanol in the reaction system effectively reduces the viscosity of the reaction system, improves the dispersibility and intermolecular contact efficiency of the reactants, and facilitates the subsequent separation and purification of the product.

[0012] In some embodiments, the signal peptide-zinc gluconate complex includes the following preparation steps: Signal peptide, zinc gluconate, and sodium citrate were dispersed in water to allow the signal peptide to undergo a complexation reaction with zinc gluconate, resulting in a signal peptide-zinc gluconate complex. The signal peptide includes at least one of myristoyl pentapeptide-4, acetyl tetrapeptide-3, and palmitoyl tripeptide-1; Through the above embodiments, the preparation method of the signal peptide-zinc gluconate complex uses the signal peptide and zinc gluconate as the core reaction components, supplemented by sodium citrate as a stabilizer, to achieve a mild complexation reaction in an aqueous system, constructing a structurally stable and hydrophilic zinc complex. Selectable signal peptides such as myristoyl pentapeptide-4, acetyl tetrapeptide-3, and palmitoyl tripeptide-1 are all functional molecules of the signal peptide class, possessing biological effects such as promoting collagen synthesis, enhancing hair follicle cell activity, and delaying hair follicle aging. Zinc gluconate, as a water-soluble carrier of zinc, not only has good skin compatibility but also provides anti-inflammatory properties. It has multiple benefits, including regulating sebum secretion and anti-oxidation. The signal peptide-zinc gluconate complex formed through the complexation reaction can enhance the stability and slow release of zinc ions in the scalp environment, avoid the potential irritation of the skin barrier by free zinc, and simultaneously enhance the structural stability and penetration efficiency of the signal peptide, synergistically exerting the effects of nutrient supply and hair follicle activation. Sodium citrate, as a stabilizer, can further regulate the pH and ionic strength of the complexation reaction system, improve the complexation efficiency of the signal peptide and zinc gluconate and the stability of the product, and ensure the dispersibility and bioavailability of the obtained signal peptide-zinc gluconate complex in scalp care products.

[0013] Furthermore, the signal peptide-zinc gluconate complex comprises the following preparation steps: 100 parts of signal peptide, 8-12 parts of zinc gluconate, and 1-3 parts of sodium citrate were dispersed in 1000-1500 parts of water and stirred at 400-600 rpm for 1-2 hours at 25-40℃ to obtain the signal peptide-zinc gluconate complex.

[0014] Through the above implementation methods, efficient complexation of signal peptides and zinc gluconate can be achieved in a mild environment, avoiding damage to the signal peptide structure under high temperature or strong acid / alkali conditions, and ensuring that its biological activity is not lost. The addition ratio of zinc gluconate can provide sufficient zinc ions for complexation while avoiding precipitation or potential scalp irritation caused by excessive zinc ions. Sodium citrate, as a complexation stabilizer, can effectively adjust the pH and ionic strength of the reaction system when introduced in an appropriate amount, improving the selectivity of the complexation reaction and the colloidal stability of the product, and preventing dissociation or aggregation of the complex during storage or use. The high proportion of water in the reaction system helps to reduce the viscosity of the reaction system, improve the dispersibility and contact efficiency of the signal peptide and zinc gluconate, and make the final signal peptide-zinc gluconate complex have good hydrophilicity and dispersibility.

[0015] In some embodiments, the citrate amide derivative includes the following preparation steps: Amino acids, citric acid, and a dehydrating condensing agent are dispersed in water to induce an amidation reaction between the amino acids and citric acid, yielding a citric acyl amino acid amide derivative. The amino acid includes at least one of arginine, glycine, methionine, and serine; the dehydrating condensing agent includes at least one of sodium tripolyphosphate, sodium pyrophosphate, and sodium dihydrogen phosphate.

[0016] Through the above-described embodiments, amino acids and citric acid are used as the core reaction components, supplemented by a dehydrating condensing agent, to achieve a mild amidation reaction in water, constructing a structurally stable and hydrophilic amide derivative. The amino acids all exhibit good biocompatibility and functional diversity, and all possess scalp barrier repair functions. Citric acid, as a tricarboxylic acid, not only provides multiple amidation reaction sites but also endows the product with good chelating ability and sustained-release properties. The citric acyl amino acid amide derivative formed through the amidation reaction can enhance the stability and skin permeability of amino acids, increasing their application potential in skincare, health care, and functional materials. The dehydrating condensing agent can efficiently promote the formation of amide bonds in the aqueous phase, avoiding the dependence on organic solvents and high-temperature conditions in traditional amidation reactions, thus improving reaction efficiency and environmental friendliness. The resulting citric acyl amino acid amide derivative has good water solubility and dispersibility, making it suitable for scalp care and functional skincare products.

[0017] Furthermore, the citrate amide derivative comprises the following preparation steps: 100 parts of amino acids, 20-40 parts of citric acid, and 5-10 parts of dehydrating condensing agent are dispersed in 1000-1500 parts of water and stirred at 400-600 rpm for 2-4 hours at 50-70℃ to obtain a citric acid amino acid amide derivative.

[0018] Through the above-described embodiments, a highly efficient amidation reaction of amino acids and citric acid can be achieved in a mild environment. The controlled addition ratio of citric acid provides sufficient carboxyl sites to promote amide bond formation while avoiding side reactions caused by excessive citric acid. The appropriate introduction of a dehydrating condensing agent can effectively promote the formation of amide bonds, while adjusting the pH and ionic strength of the reaction system, improving reaction selectivity and product structural stability, and preventing hydrolysis or aggregation of amide derivatives during storage or use. A higher proportion of water in the reaction system helps to reduce system viscosity, enhance the dispersibility and intermolecular contact efficiency of amino acids and citric acid, and give the final citrate amino acid amide derivative good hydrophilicity, dispersibility, and application adaptability.

[0019] Secondly, this application provides a method for preparing an anti-hair loss and hair-strengthening scalp care essence, comprising the following preparation steps: Provide the raw materials for the anti-hair loss and hair-strengthening scalp care essence according to any embodiment of the first aspect; The raw materials are mixed to obtain an anti-hair loss and hair-strengthening scalp care essence.

[0020] Through the above-described implementation methods, the scientific selection and proportioning of raw materials ensure that each functional ingredient can play its full role in subsequent steps, laying the foundation for the preparation of the anti-hair loss and hair-strengthening scalp care essence; the desired anti-hair loss and hair-strengthening scalp care essence is obtained by mixing the raw materials.

[0021] Furthermore, the method for preparing an anti-hair loss and hair-strengthening scalp care essence includes the following preparation steps: S1: Providing the raw materials for the anti-hair loss and hair-strengthening scalp care essence according to any one of claims 1-8; S2: At 20-30℃, mix water, humectant, solubilizer, organic acid, preservative and plant extract, and stir at 300-500 rpm for 20-30 min to obtain the basic aqueous phase; S3: At 20-30℃, the basic aqueous phase is mixed with caffeine, yeast extract and wood oil, and stirred at 300-500 rpm for 10-20 minutes to obtain the anti-hair loss and hair strengthening scalp care essence mother liquor. S4: Under conditions of 20-30℃, the hair loss prevention and hair strengthening scalp care essence mother liquor, nicotinamide-citramide-flavonoid diamide derivative, signal peptide-zinc gluconate complex, and citramide amino acid amide derivative are mixed and stirred at a rate of 500-700 rpm for 30-40 minutes. After filtration to remove impurities, the hair loss prevention and hair strengthening scalp care essence is obtained.

[0022] Through the above implementation methods, a phased, gradual addition and gentle stirring process strategy is adopted to improve the dispersion efficiency and system stability of each component in the anti-hair loss and scalp strengthening scalp care essence, ensuring the homogeneity and bioactivity of the final anti-hair loss and scalp strengthening scalp care essence. Specifically, in step S2, water, humectant, solubilizer, organic acid, preservative, and plant extracts are premixed to form a basic aqueous phase, which helps to build a stable hydrophilic environment and improve the solubility of subsequent components and the pH buffering capacity of the system. In step S3, caffeine, yeast extract, and costus root oil are introduced into the basic aqueous phase under gentle conditions, which avoids the degradation of caffeine and yeast active ingredients due to excessively high temperatures, while ensuring that the aroma and function of volatile components such as costus root oil are not destroyed, resulting in a structurally homogeneous and odor-stable essence. The mother liquor for anti-hair loss and hair-strengthening scalp care essence; under the conditions of step S4, functional active ingredients such as nicotinamide-citryl flavonoid diamide derivative, signal peptide-zinc gluconate complex, and citryl amino acid amide derivative are introduced and thoroughly stirred at an appropriate stirring rate, which helps to improve the dispersibility and synergistic stability of the functional active ingredients and avoids problems such as aggregation, precipitation, or phase separation caused by direct high-concentration addition; the above step-by-step addition strategy can effectively protect the integrity of heat-sensitive and structure-sensitive active substances in the anti-hair loss and hair-strengthening scalp care essence during the preparation process, ensuring their bioactivity and penetration efficiency in the final product; the final anti-hair loss and hair-strengthening scalp care essence has good stability, dispersibility, and bioavailability, and can prevent hair loss and increase hair density.

[0023] Thirdly, this application provides a scalp care essence for preventing hair loss and strengthening hair, prepared according to the method described in the first aspect or the method described in the second aspect, which is suitable for scalp care fields such as preventing hair loss, strengthening hair and nourishing hair, repairing scalp barrier function and conditioning hair follicle activity.

[0024] Compared with the prior art, the beneficial effects of this application are at least as follows: This invention utilizes the synergistic effects of functional derivatives such as nicotinamide-citryl flavonoid diamide derivatives, signal peptide-zinc gluconate complexes, and citryl amino acid amide derivatives, constructed through amidation and complexation reactions, to build a multi-pathway mechanism for preventing hair loss and strengthening hair. Furthermore, these functional derivatives possess excellent water solubility and structural stability, enhancing the penetration efficiency and bioavailability of active ingredients in the scalp environment, overcoming the problems of easy degradation and poor absorption associated with traditional ingredients. The entire formulation system employs green processes such as gentle aqueous phase reaction, low-temperature stirring, and enzyme catalysis to avoid damage to active substances, ensuring the product's good adaptability and safety for people with sensitive scalps. Detailed Implementation

[0025] The various embodiments or implementation schemes in this specification are described in a progressive manner, with each embodiment focusing on the differences from other embodiments.

[0026] In the description of this specification, the references to terms such as "one embodiment," "some embodiments," "illustrative embodiment," "example," "specific example," or "some examples," etc., indicate that a specific feature, structure, material, or characteristic described in connection with an embodiment or example is included in at least one embodiment or example of this application. In this specification, the illustrative expressions of the above terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in one or more embodiments or examples.

[0027] Furthermore, the terms "first" and "second" are used for descriptive purposes only and should not be construed as indicating or implying relative importance or implicitly specifying the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one of that feature. In the description of this application, "multiple" means at least two, such as two, three, etc., unless otherwise explicitly specified.

[0028] In this manual, unless otherwise specified, "parts" refers to "parts by mass" and "rpm" refers to "revolutions per minute".

[0029] The following describes embodiments of this application. The embodiments described below are exemplary and are only used to explain this application, and should not be construed as limiting this application. Where specific techniques or conditions are not specified in the embodiments, they are performed according to the techniques or conditions described in the literature in the art or according to the product instructions. Reagents or instruments used, unless otherwise specified, are all conventional products that comply with the "Cosmetic Safety Technical Specifications" and can be obtained commercially.

[0030] Lactic acid, CAS number 50-21-5, effective lactic acid content ≥88%, the remainder is water; The extract of Platycladus orientalis leaves, in the form of an aqueous extract, at a 1:10 extraction ratio, contained ≥0.3% volatile oil and ≥1% total flavonoids; Tea extract, in the form of an aqueous extract, at a 1:10 extraction ratio, contains ≥20% tea polyphenols and ≥1.5% caffeine; Anemarrhena asphodeloides root extract, in the form of an aqueous extract, at a 1:10 extraction ratio, contains total saponins ≥2% and mangiferin ≥0.5%; Asparagus root extract, in the form of an aqueous extract, at a 1:10 extraction ratio, contains ≥2% total saponins. Ginseng root extract, in the form of an aqueous extract, at a 1:10 extraction ratio, contains ≥5% total saponins; Ginger root extract, in the form of an aqueous extract, at a 1:10 extraction ratio, contains gingerol ≥0.5% and total polyphenols ≥2%; Angelica sinensis root extract, in the form of an aqueous extract, at a 1:10 extraction ratio, contains ferulic acid ≥0.1% and total polysaccharides ≥5%. Polygonum multiflorum root extract, in the form of an aqueous extract, at a 1:10 extraction ratio, contains stilbene glycosides ≥1% and total anthraquinones ≥0.5%; European horse chestnut seed extract, in the form of an aqueous extract, at a 1:10 extraction ratio, contains aescin A ≥3% and total flavonoids ≥1%; Novozymes lipase 435 is selected from Novozymes lipase 435 of Shanghai Yuanye Biotechnology Co., Ltd.; Myristoyl pentapeptide-4, CAS number 1392416-25-9; Acetyl tetrapeptide-3, CAS number 827306-88-7; Palmitoyl tripeptide-1, CAS number 147732-56-7; Woody oil, CAS number 8023-88-9; Yeast extract, CAS No. 8013-01-2.

[0031] Preparation Example 1 Preparation of nicotinamide-citryl flavonoid diamide derivatives: M1: 100 parts of 5,7-dihydroxyflavone, 20 parts of citric acid, and 5 parts of Novozymes lipase 435 were dispersed in 600 parts of isopropanol and stirred at 400 rpm for 6 h at 50 °C to obtain a dispersion of carboxylated flavonoid derivatives. M2: The dispersion of the carboxylated flavonoid derivative was mixed with 100 parts of nicotinamide and stirred at 450 rpm for 10 h at 50 °C. The mixture was then allowed to cool naturally to room temperature and allowed to settle for 1.5 h. The lower precipitate was discarded. The supernatant was then rotary evaporated at -0.08 MPa and 45 °C for 1.5 h to obtain the nicotinamide-citryl flavonoid diamide derivative.

[0032] Preparation Example 2 Preparation of signal peptide-zinc gluconate complex: 100 parts of signal peptide, 9 parts of zinc gluconate, and 2.5 parts of sodium citrate were dispersed in 1200 parts of water and stirred at 500 rpm for 1.5 h at 30 °C. The mixture was then allowed to cool naturally to room temperature and allowed to settle for 1 h. The lower precipitate was discarded to obtain the signal peptide-zinc gluconate complex.

[0033] Preparation Example 3 Preparation of citrate acyl amino acid amide derivatives: 100 parts of arginine, 30 parts of citric acid, and 6.5 parts of sodium dihydrogen phosphate were dispersed in 1100 parts of water and stirred at 500 rpm for 4 hours at 60°C. The mixture was then allowed to cool naturally to room temperature and allowed to settle for 1 hour. The lower precipitate was then discarded to obtain a citrate amino acid amide derivative.

[0034] Example 1 Preparation of a hair loss prevention and hair strengthening scalp care essence: S1: Provides 50 parts water; humectants: 6 parts butylene glycol, 4 parts 1,2-pentanediol; solubilizers: 0.5 parts disodium EDTA, 1.5 parts PEG-40 hydrogenated castor oil; organic acid: 0.55 parts citric acid; preservative: 0.5 parts p-hydroxyacetophenone; plant extracts: 0.6 parts Platycladus orientalis leaf extract, 0.6 parts Anemarrhena asphodeloides root extract, 0.6 parts ginseng root extract, 0.6 parts ginger root extract, 0.6 parts Angelica sinensis root extract, 0.6 parts Polygonum multiflorum root extract, 0.6 parts Aesculus hippocastanum seed extract; 4.2 parts of nicotinamide-citryl flavonoid diamide derivative of Preparation Example 1; 18 parts of signal peptide-zinc gluconate complex of Preparation Example 2; 32 parts of citryl amino acid amide derivative of Preparation Example 3; 0.4 parts caffeine; 1.8 parts yeast extract; 0.12 parts costus root oil.

[0035] S2: At 25°C, water, humectant, solubilizer, organic acid, preservative and plant extract are mixed and stirred at 400 rpm for 30 min to obtain the basic aqueous phase. S3: At 25°C, the basic aqueous phase is mixed with caffeine, yeast extract and wood oil, and stirred at 400 rpm for 15 minutes to obtain the anti-hair loss and hair strengthening scalp care essence mother liquor. S4: At 25°C, the hair loss prevention and hair strengthening scalp care essence mother liquor, nicotinamide-citramide-flavonoid diamide derivative, signal peptide-zinc gluconate complex, and citramide amino acid amide derivative are mixed and stirred at 600 rpm for 30 minutes. The mixture is then coarsely filtered through a 200-mesh stainless steel sieve and finely filtered through a 10 μm filter membrane to obtain the hair loss prevention and hair strengthening scalp care essence.

[0036] Comparative Example 1 The formulation is largely the same as Example 1, except that 4.2 parts of the nicotinamide-citryl flavonoid diamide derivative of Example 1 are replaced with 1.87 parts of 5,7-dihydroxyflavone, 0.37 parts of citric acid, 0.09 parts of Novozymes lipase 435, and 1.87 parts of nicotinamide.

[0037] Comparative Example 2 The formulation is largely the same as Example 1, except that 18 parts of the signal peptide-zinc gluconate complex prepared in Example 2 are replaced with 1.37 parts of signal peptide, 0.12 parts of zinc gluconate, 0.03 parts of sodium citrate, and 16.48 parts of water.

[0038] Comparative Example 3 The formulation is largely the same as Example 1, except that 32 parts of the citrate amino acid amide derivative of Example 3 are replaced with 2.59 parts of arginine, 0.78 parts of citric acid, 0.17 parts of sodium dihydrogen phosphate, and 28.46 parts of water.

[0039] Test section Methods for testing the effectiveness of hair loss prevention and hair strengthening: Subjects meeting the requirements for hair loss prevention efficacy testing according to the "Cosmetic Safety Technical Specifications" (2015 edition) were selected. Those with more than 10 hairs lost were screened using the 60-comb method and were qualified subjects. Qualified subjects first underwent a 2-week shampooing period, during which they uniformly used base formula products without hair loss prevention and hair strengthening ingredients. After the shampooing period, they were screened again. Those with more than 10 hairs lost still entered the formal trial. Hair density detection: A 1.5cm × 1.5cm hair cutting area was fixed on one side of the top of the head. During each visit, the hair was cut until the remaining length was ≤1mm. Local images were taken using a dermatoscope, and the hair density was analyzed through the images. In the formal trial, participants were randomly assigned to four groups using a stratified randomization method: Example 1, Comparative Example 1, Comparative Example 2, and Comparative Example 3. Each group had 30 participants. Potential influencing factors such as race, gender, age, hair length, and severity of hair loss were balanced during group assignment. The mean age of participants in each group was 33.4 years, and the standard deviation of age was 5.3 years to ensure baseline comparability between groups. Hair density was measured and recorded for each group before the trial, and standardized images were taken. The above tests were repeated after continuous use of the corresponding anti-hair loss and hair-strengthening scalp care essence for 4, 8, and 12 weeks, respectively. The ambient temperature was 20℃, the relative humidity was 50%, and the lighting conditions were constant (LED light with a color temperature of 6000K). The final result was the average value of each subject and the standard deviation was calculated.

[0040] Table 1 shows the hair density test results before the experiment and after continuous use of the corresponding anti-hair loss and hair strengthening scalp care serum for 4, 8, and 12 weeks: Table 1

[0041] With 58 degrees of freedom, a one-tailed test was performed, α=0.05, and the critical value was 1.671. When t>1.671, the difference was statistically significant (p<0.05). The change in hair density for each group was calculated as follows: Δ = hair density after 12 weeks - hair density before use. The changes in hair density over 12 weeks between Example 1 and each comparative example were systematically evaluated using analysis of difference (Δ method), as shown in Table 2. Table 2

[0042] As shown in Table 1, Example 1 of the present invention showed superior hair density compared to the comparative example after 4 weeks, 8 weeks, and 12 weeks. Furthermore, as shown in Table 2, the anti-hair loss and hair-strengthening scalp care essence of Example 1 exhibited a statistically significant advantage over the comparative example at 12 weeks, verifying the synergistic effect of the nicotinamide-citryl flavonoid diamide derivative of Example 1, the signal peptide-zinc gluconate complex of Example 2, and the citryl amino acid amide derivative of Example 3 in the anti-hair loss and hair-strengthening scalp care essence. This may be because the nicotinamide-citryl flavonoid diamide derivative, the signal peptide-zinc gluconate complex, and the citryl amino acid amide derivative synergistically construct a multi-pathway linkage mechanism for preventing hair loss and strengthening hair in the scalp care system; the nicotinamide-citryl flavonoid diamide derivative, through diamide bonds... The interconnected molecular framework enhances its dispersibility and permeability in aqueous systems. Its flavonoid structure endows it with antioxidant and anti-inflammatory capabilities. The nicotinamide fragment can regulate scalp sebum secretion and repair scalp barrier function, improve the hair follicle microenvironment, delay hair follicle atrophy, and prolong the hair growth cycle. The zinc ions in the signal peptide-zinc gluconate complex achieve sustained release through the complexation structure, avoiding the stimulation of the scalp by free zinc, and enhancing the structural stability and bioavailability of the signal peptide, promoting hair follicle metabolism and keratin synthesis. The citrate amino acid amide derivative can build a hydrophilic barrier on the scalp surface by mimicking the structure of natural moisturizing factors. Its amide bond structure enhances molecular stability and hydrophilicity, helps to enhance the tolerance of the scalp stratum corneum and relieve discomfort such as dryness and itching, thereby further improving the stability of the scalp environment and hair follicle activity.

[0043] As shown in Table 1, Example 1 showed superior hair density compared to Comparative Example 1 after 4 weeks, 8 weeks, and 12 weeks. Furthermore, Table 2 shows that the anti-hair loss and hair-strengthening scalp care essence of Example 1 exhibited a statistically significant advantage over Comparative Example 1 at 12 weeks, verifying the synergistic effect of the nicotinamide-citryl flavonoid diamide derivative of Example 1 in the anti-hair loss and hair-strengthening scalp care essence. This is likely because the nicotinamide-citryl flavonoid diamide derivative introduced in Example 1 not only enhances the overall water solubility and dispersibility of the molecule but also constructs a multi-target synergistic functional pathway in the scalp environment. The flavonoid structure in this nicotinamide-citryl flavonoid diamide derivative possesses excellent antioxidant and anti-inflammatory capabilities. It can effectively scavenge free radicals, inhibit the expression of inflammatory factors, and improve the microenvironment around hair follicles. At the same time, niacinamide fragments can regulate sebum secretion, promote microcirculation, and repair the skin barrier, thereby delaying hair follicle atrophy, prolonging the hair growth cycle, and improving hair density and growth activity. Moreover, its amide bond structure enhances the hydrogen bonding between molecules, reduces the risk of aggregation and sedimentation, and ensures the uniform distribution and continuous release of active ingredients on the scalp surface. Although Comparative Example 1 contains the same raw materials, it has not undergone amidation derivatization treatment and lacks the enhancing effect of the diamide structure on molecular polarity. The dispersibility and permeability of active ingredients in the anti-hair loss and hair strengthening scalp care essence are limited, resulting in an uneven functional layer formed on the scalp surface, making it difficult to achieve continuous and effective release of bioactivity, which is reflected in the limited increase in hair density.

[0044] As shown in Table 1, Example 1 showed significantly better hair density than Comparative Example 2 after 4 weeks, 8 weeks, and 12 weeks. Furthermore, Table 2 shows that the anti-hair loss and hair-strengthening scalp care essence of Example 1 had a statistically significant advantage over Comparative Example 2 at 12 weeks, verifying the synergistic effect of the signal peptide-zinc gluconate complex in the anti-hair loss and hair-strengthening scalp care essence of Example 2. This may be because Example 1 used a pre-prepared signal peptide-zinc gluconate complex, which forms a stable composite structure through a complexation reaction, enhancing... The slow-release capacity and bioavailability of zinc ions avoid the potential irritation of the scalp caused by free zinc, and enhance the structural stability and functional sustainability of the signal peptide, thus being more conducive to promoting hair follicle cell metabolism, activating hair growth pathways, and improving keratin synthesis efficiency. In contrast, the signal peptide directly added in Comparative Example 2 did not form a stable complex structure with zinc gluconate, resulting in uneven release of zinc ions, easy irritation to the scalp, and easy degradation of the signal peptide, limiting its biological activity and making it difficult to fully exert its synergistic effect in preventing hair loss and strengthening hair, resulting in a limited increase in hair density.

[0045] As shown in Table 1, Example 1 showed significantly better hair density than Comparative Example 3 after 4 weeks, 8 weeks, and 12 weeks. Furthermore, Table 2 shows that the anti-hair loss and hair-strengthening scalp care essence of Example 1 had a statistically significant advantage over Comparative Example 3 at 12 weeks, verifying the synergistic effect of the citrate amide derivative in Example 3 in the anti-hair loss and hair-strengthening scalp care essence. This may be because Example 1 used a pre-prepared citrate amide derivative, which constructed a stable amide bond structure through an amidation reaction, enhancing its hydrophilicity and stability. Qualitative analysis also simulated the structural characteristics of natural moisturizing factors, which can form a uniform hydrophilic barrier on the scalp surface, effectively relieving discomfort such as dryness and itching, and can also enhance the tolerance and barrier function of the stratum corneum, thereby optimizing the scalp microenvironment and improving hair follicle activity. In contrast, the arginine, citric acid and sodium dihydrogen phosphate added directly in Comparative Example 3 did not form stable amide derivatives. The intermolecular forces in the system were weak, and it was easily diluted or metabolized, making it difficult to form a continuous and effective functional layer on the scalp surface. As a result, its synergistic effect in improving the scalp environment and promoting hair growth was insufficient, which was reflected in the limited increase in hair density.

[0046] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of this application, and are not intended to limit them. Although this application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some or all of the technical features therein. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of this application.

Claims

1. A hair loss prevention and hair strengthening scalp care essence, characterized in that, Includes the following quantities of raw materials: 50 parts water; 8-12 parts of moisturizer; 1-3 parts of co-solvent; Organic acids, 0.3-0.6 parts; Preservative 0.5-1 part; 2-5 parts plant extract; Nicotinamide-citryl flavonoid diamide derivative, 3.5-4.5 parts; 15-20 parts of signal peptide-zinc gluconate complex; 25-35 parts of citrate yl amino acid amide derivative; Caffeine 0.3-0.6 parts; 1-2 parts yeast extract; 0.1-0.3 parts of wood oil.

2. The anti-hair loss and hair-strengthening scalp care essence according to claim 1, characterized in that, The hair loss prevention and hair strengthening scalp care serum meets at least one of the following conditions: 1) The moisturizer includes at least one of butylene glycol, 1,2-pentanediol, propylene glycol, 1,2-hexanediol, and octyl glycol; 2) The cosolvent includes at least one of disodium EDTA, PEG-6 octanoic acid / capric acid glycerides, and PEG-40 hydrogenated castor oil; 3) The organic acid includes at least one of citric acid and lactic acid; 4) The preservative includes at least one of phenoxyethanol and p-hydroxyacetophenone; 5) The plant extracts include at least one of the following: Platycladus orientalis leaf extract, Anemarrhena asphodeloides root extract, Ginseng root extract, Ginger root extract, Angelica sinensis root extract, Polygonum multiflorum root extract, and Horse chestnut seed extract.

3. The anti-hair loss and hair-strengthening scalp care essence according to claim 1, characterized in that, The nicotinamide-citryl flavonoid diamide derivative comprises the following preparation steps: M1: Flavonoids, citric acid, and Novozymes lipase 435 are dispersed in isopropanol, and the flavonoids and citric acid undergo an amidation reaction to obtain a dispersion of carboxylated flavonoid derivatives. M2: The dispersion of the carboxylated flavonoid derivative is mixed with nicotinamide to induce an amidation reaction between the carboxylated flavonoid derivative and nicotinamide, thereby obtaining a nicotinamide-citryl flavonoid diamide derivative. The flavonoids include at least one of rutin, quercetin, naringin, and 5,7-dihydroxyflavone.

4. The anti-hair loss and hair-strengthening scalp care essence according to claim 3, characterized in that, The nicotinamide-citryl flavonoid diamide derivative comprises the following preparation steps: M1: Disperse 100 parts of flavonoids, 10-30 parts of citric acid, and 5-10 parts of Novozymes lipase 435 in 500-800 parts of isopropanol, and stir at 300-500 rpm for 4-8 hours at 40-60℃ to obtain a dispersion of carboxylated flavonoid derivatives. M2: Mix the dispersion of the carboxylated flavonoid derivative with 80-120 parts of nicotinamide and stir at 400-600 rpm for 8-12 h at 40-60℃ to obtain nicotinamide-citryl flavonoid diamide derivative.

5. The anti-hair loss and hair-strengthening scalp care essence according to claim 1, characterized in that, The signal peptide-zinc gluconate complex comprises the following preparation steps: Signal peptide, zinc gluconate, and sodium citrate were dispersed in water to allow the signal peptide to undergo a complexation reaction with zinc gluconate, resulting in a signal peptide-zinc gluconate complex. The signal peptide includes at least one of myristoyl pentapeptide-4, acetyl tetrapeptide-3, and palmitoyl tripeptide-1.

6. The anti-hair loss and hair-strengthening scalp care essence according to claim 5, characterized in that, The signal peptide-zinc gluconate complex comprises the following preparation steps: 100 parts of signal peptide, 8-12 parts of zinc gluconate, and 1-3 parts of sodium citrate were dispersed in 1000-1500 parts of water and stirred at 400-600 rpm for 1-2 hours at 25-40℃ to obtain the signal peptide-zinc gluconate complex.

7. The anti-hair loss and hair-strengthening scalp care essence according to claim 1, characterized in that, The citrate yl amino acid amide derivative comprises the following preparation steps: Amino acids, citric acid, and a dehydrating condensing agent are dispersed in water to induce an amidation reaction between the amino acids and citric acid, yielding a citric acyl amino acid amide derivative. The amino acid includes at least one of arginine, glycine, methionine, and serine; the dehydrating condensing agent includes at least one of sodium tripolyphosphate, sodium pyrophosphate, and sodium dihydrogen phosphate.

8. The anti-hair loss and hair-strengthening scalp care essence according to claim 7, characterized in that, The citrate yl amino acid amide derivative comprises the following preparation steps: 100 parts of amino acids, 20-40 parts of citric acid, and 5-10 parts of dehydrating condensing agent are dispersed in 1000-1500 parts of water and stirred at 400-600 rpm for 2-4 hours at 50-70℃ to obtain a citric acid amino acid amide derivative.

9. A method for preparing an anti-hair loss and hair-strengthening scalp care essence, characterized in that, The preparation steps include the following: Provide the raw materials for the anti-hair loss and hair-strengthening scalp care essence according to any one of claims 1-8; The raw materials are mixed to obtain an anti-hair loss and hair-strengthening scalp care essence.

10. The method according to claim 9, characterized in that, The preparation steps include the following: S1: Providing the raw materials for the anti-hair loss and hair-strengthening scalp care essence according to any one of claims 1-8; S2: At 20-30℃, mix water, humectant, solubilizer, organic acid, preservative and plant extract, and stir at 300-500 rpm for 20-30 min to obtain the basic aqueous phase; S3: At 20-30℃, the basic aqueous phase is mixed with caffeine, yeast extract and wood oil, and stirred at 300-500 rpm for 10-20 minutes to obtain the anti-hair loss and hair strengthening scalp care essence mother liquor. S4: Under conditions of 20-30℃, the hair loss prevention and hair strengthening scalp care essence mother liquor, nicotinamide-citramide-flavonoid diamide derivative, signal peptide-zinc gluconate complex, and citramide amino acid amide derivative are mixed and stirred at a rate of 500-700 rpm for 30-40 minutes. After filtration to remove impurities, the hair loss prevention and hair strengthening scalp care essence is obtained.

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