Double-label nuclide analysis method, device and program product

Abstract

The embodiment of the invention relates to the technical field of nuclear detection and measurement, and discloses a double-label nuclide analysis method and device and a program product, and the method comprises the steps: measuring a standard quenching source, and obtaining an external standard source spectrum index of the standard quenching source; drawing a correction curve by taking the external standard source spectrum index as an independent variable; measuring the double-labeled sample under external gamma source high voltage, and obtaining and calculating an external standard source spectrum index of the double-labeled sample through external gamma source spectrum stripping; determining the counting efficiency of low-energy nuclide, the counting efficiency of high-energy nuclide, the spectrum index of the low-energy nuclide sample and the spectrum index value of the high-energy nuclide sample corresponding to the double-labeled sample under the current quenching level according to the external standard source spectrum index of the double-labeled sample and the correction curve; the method comprises the following steps: measuring a double-labeled sample under double-labeled high pressure to obtain a full-spectrum counting rate and a full-spectrum sample spectrum index of the double-labeled sample; the low-energy nuclide activity and the high-energy nuclide activity of the double-labeled sample are calculated based on the parameters, so that the operability is higher, the accuracy is higher, and the application range is wider.

Description

Technical Field

[0001] This application relates to the field of nuclear detection and measurement technology, and in particular to tracer technology, and relates to a dual-labeled nuclide analysis method, equipment and program product. Background Technology

[0002] Currently, in the field of nuclear science and technology, especially in radiation measurements using liquid scintillator spectrometers, qualitative and quantitative analysis are employed. Qualitative analysis refers to distinguishing and identifying specific nuclides present in a sample by measuring the characteristic energy of gamma rays emitted by different nuclides or the energy spectrum of beta particles. Quantitative analysis refers to calculating the activity concentration of nuclides in a sample by measuring the intensity of radioactivity (count rate) and combining it with detection efficiency and nuclide decay parameters. There are two main methods for determining the activity of two beta nuclides (i.e., dual-labeled nuclides) within a single sample: the inclusion method and the full-spectrum DPM method.

[0003] The traditional inclusion method separates high-energy and low-energy nuclides by setting discrimination thresholds (LL and UL) in the counting region. This method requires defining a boundary between low-energy and high-energy nuclides. However, in practical applications, this boundary is affected by the degree of sample quenching, leading to difficulty and poor accuracy in the delineation process, thus increasing the error in solving for dual-labeled nuclides. Furthermore, this method has limitations when nuclides have similar maximum energies, resulting in poor applicability.

[0004] Another method is the full-spectrum DPM method, developed by Packard Instruments. Its principle is to decompose the composite spectrum into two components and determine the count contribution of each radionuclide to the total activity using the Sample Spectral Index (SIS). When establishing the quenching correction curve, the LSA collects the Sample Spectral Index and the percentage count efficiencies of low-energy and high-energy nuclide standards, and uses the quenching indication parameters generated by the sample itself to plot the correction curve. However, a key drawback of the full-spectrum DPM method is that the acquisition of its quenching indication parameters depends on the energy spectrum and counts generated by the sample source. This dependence leads to decreased accuracy of the quenching indication parameters for low-activity samples or samples with a low proportion of a particular nuclide. Therefore, the full-spectrum DPM method has strict requirements on the solvable range and limited applicability. Summary of the Invention

[0005] One objective of this application is to provide a dual-labeled nuclide analysis method, apparatus, and procedure product, which at least solves the problem that the existing full-spectrum DPM method relies on the sample's own activity and count, resulting in poor accuracy and limited applicability for dual-labeled samples with low activity or large differences in nuclide content ratio.

[0006] To achieve the above objectives, some embodiments of this application provide the following aspects:

[0007] In a first aspect, this application provides a dual-labeled nuclide analysis method, which is used to determine the activities of low-energy and high-energy nuclides in a liquid scintillation spectrometer, the method comprising:

[0008] Measure the standard quenching source and obtain the external standard source spectrum index of the standard quenching source;

[0009] Using the external standard source spectral index of the standard quenching source as the independent variable, and the low-energy nuclide counting efficiency, high-energy nuclide counting efficiency, low-energy nuclide sample spectral index, and high-energy nuclide sample spectral index of the standard quenching source as the dependent variables, multiple sets of paired data points are formed; mathematical fitting is performed on the data points to establish the functional relationship between the independent variable and the dependent variable, and a calibration curve is plotted.

[0010] The double-labeled sample was measured under high pressure from an external γ source, and the external standard source spectral index of the double-labeled sample was obtained and calculated by external γ source stripping.

[0011] Based on the external standard source spectral index of the dual-labeled sample and the calibration curve, determine the counting efficiency of low-energy nuclides, the counting efficiency of high-energy nuclides, the sample spectral index of low-energy nuclides, and the sample spectral index of high-energy nuclides corresponding to the dual-labeled sample at the current quenching level.

[0012] Under dual-labeled high pressure, the dual-labeled sample is measured to obtain the full-spectrum count rate and full-spectrum sample index of the dual-labeled sample;

[0013] Based on the full-spectrum count rate, full-spectrum sample index, count efficiency of low-energy nuclides, count efficiency of high-energy nuclides, low-energy nuclide sample index, and high-energy nuclide sample index of the dual-labeled sample, the low-energy nuclide activity and high-energy nuclide activity of the dual-labeled sample are calculated.

[0014] Secondly, some embodiments of this application also provide an electronic device, the electronic device comprising: one or more processors; and a memory storing computer program instructions, which, when executed, cause the processor to perform the steps of the method described above.

[0015] Thirdly, some embodiments of this application also provide a computer program product, including a computer program / instructions that, when executed by a processor, implement the steps of the method described above.

[0016] Compared with related technologies, the solution provided in this application uses the pure spectrum index (ES_SIS) of an external standard source as the quenching indicator parameter and a stable and high-activity external gamma source to establish calibration curves for high- and low-energy nuclides. This decouples the activity calculation from the sample's own activity, overcoming the shortcomings of the traditional full-spectrum DPM method. When solving for the quenching degree and efficiency of low- and high-energy nuclides, since ES_SIS is calculated based on a stable external gamma source signal, the entire solution process does not depend on the sample's own activity and count. This effectively solves the technical problem of poor accuracy of the quenching indicator parameter for low-activity samples or samples with a low proportion of nuclides in the prior art. Furthermore, this method has higher accuracy and a wider range of applications. Because the calibration curve is established by a stable external gamma source, the accuracy of the parameters is guaranteed, resulting in higher accuracy of the activity calculation. At the same time, this method has no special requirements on the sample's activity range and nuclide content ratio, greatly expanding the applicability of the dual-labeled nuclide analysis method. It is more operable in practical applications, improving user convenience. Attached Figure Description

[0017] One or more embodiments are illustrated by way of example with reference numerals in the accompanying drawings. These illustrations do not constitute a limitation on the embodiments. Elements with the same reference numerals in the drawings are denoted as similar elements. Unless otherwise stated, the figures in the drawings are not to be limited by scale.

[0018] Figure 1 A flowchart of a dual-labeled nuclide analysis method provided as an exemplary embodiment of this disclosure;

[0019] Figure 2 A schematic diagram of a correction curve provided for an exemplary embodiment of this disclosure;

[0020] Figure 3 A flowchart illustrating the acquisition of some parameters in a dual-labeled nuclide analysis method provided as an exemplary embodiment of this disclosure;

[0021] Figure 4 A flowchart illustrating the acquisition of some parameters in another dual-labeled nuclide analysis method provided as an exemplary embodiment of this disclosure;

[0022] Figure 5 A flowchart illustrating the acquisition of some parameters in another dual-labeled nuclide analysis method provided as an exemplary embodiment of this disclosure;

[0023] Figure 6 An exemplary structural diagram of the electronic device provided for some embodiments of this application. Detailed Implementation

[0024] To make the objectives, technical solutions, and advantages of the embodiments of this application clearer, the technical solutions of the embodiments of this application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this application, not all embodiments. Based on the embodiments of this application, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of this application.

[0025] The following terms are used in this document:

[0026] ES_SIS is the external standard source spectrum index.

[0027] A L Low-energy nuclide activity

[0028] A H For high-energy nuclide activity

[0029] cpm L+H Full spectrum count rate

[0030] SIS L+H For full-spectrum sample spectral index

[0031] E L Low-energy nuclide counting efficiency for dual-labeled samples

[0032] E H High-energy nuclide counting efficiency for dual-labeled samples

[0033] SIS L Spectral index of low-energy nuclide samples

[0034] SIS H Spectral index of high-energy nuclide samples

[0035] EFF L Low-energy nuclide counting efficiency of standard quenching sources

[0036] EFF H High-energy nuclide counting efficiency of standard quenching sources

[0037] Figure 1 A flowchart illustrating a dual-labeled nuclide analysis method provided as an exemplary embodiment of this disclosure, the method being used to determine the activities of low-energy and high-energy nuclides in a liquid scintillation spectrometer, the method comprising:

[0038] S101. Measure the standard quenching source and obtain the external standard source spectrum index ES_SIS of the standard quenching source.

[0039] Specifically, preparation before measurement is necessary. The nuclide to be measured can be chosen by the user and confirmed through the manufacturer's instruction manual. The manual will provide recommended values ​​based on commonly used dual-labeled measurement objects. Similar nuclides. The measurement mode can be selected to use the external gamma spectral index curve to establish the quenching curve. Samples can be selected by the user, but should be no fewer than 5. When the "Dual-label Quenching Correction Curve" option is selected for the external gamma source, the external gamma source is automatically selected and cannot be changed. The irradiation duration is 30 seconds by default, but can be changed by the user. Afterwards, prepare a series of quenching sources, input the information and measurement parameters of the series of standard sources, establish the quenching curve measurement task, and begin the automatic measurement process of establishing the external gamma source quenching curve, thereby obtaining the external standard source spectral index ES_SIS of the standard quenching source.

[0040] S102. Using the external standard source spectral index ES_SIS of the standard quenching source as the independent variable, and the low-energy nuclide counting efficiency EFF of the standard quenching source... L High-energy nuclide counting efficiency (EFF) H Low-energy nuclide sample spectral index (SIS) L and high-energy nuclide sample spectral index SIS H As the dependent variable, multiple sets of paired data points are formed; mathematical fitting is performed on the data points to establish the functional relationship between the independent variable and the dependent variable, and a correction curve is plotted.

[0041] Specifically, this step requires drawing ES_SIS-SIS. L ES_SIS-EFF L ES_SIS-SIS H ES_SIS-EFF H Curve (e.g.) Figure 2 The curve is plotted by mathematically fitting the ES_SIS (stable quenching indicator parameters) of a series of standard quenching sources as independent variables and the EFF and SIS values ​​(the true parameters required for activity calculation) accurately measured at the same quenching level as dependent variables. This fitting process transforms the originally discrete experimental data points into a continuous functional relationship, thus binding the quenching indicator to the actual measured parameters. Once the curve is established, it becomes a mathematical model.

[0042] S103. Measure the double-labeled sample under high pressure from an external γ source, and obtain and calculate the external standard source index ES_SIS of the double-labeled sample by external γ source stripping.

[0043] Specifically, the spectral stripping measurement process in step S101 is repeated for the unknown dual-labeled sample. Under high pressure from an external γ source, the dual-labeled sample is measured, and the current external standard source index ES_SIS of the dual-labeled sample is calculated as the sole basis for subsequent parameter lookup.

[0044] S104. Based on the external standard source spectral index of the dual-labeled sample and the calibration curve, determine the counting efficiency E of the low-energy nuclide corresponding to the dual-labeled sample at the current quenching level. L The counting efficiency E of high-energy nuclides H Low-energy nuclide sample spectral index (SIS) L and the spectral index value (SIS) of high-energy nuclides H ;

[0045] Specifically, when measuring unknown dual-labeled samples, only their ES_SIS needs to be measured (this value is based on a stable external gamma source signal and does not depend on sample activity). Substitute this ES_SIS value into (ES_SIS, SIS) L (ES_SIS, SIS) H (ES_SIS, EFF) L (ES_SIS, EFF) H These four calibration curves can accurately locate or calculate the low-energy nuclide counting efficiency E corresponding to the current quenching level of the sample. L High-energy nuclide counting efficiency E H Low-energy nuclide sample spectral index (SIS) L and high-energy nuclide sample spectral index SIS H This ensures that the key parameters required for activity calculation are not affected by low activity or content ratio in the sample, thereby improving the accuracy of the final activity calculation and the applicability of the method.

[0046] S105. Under dual-labeled high pressure, measure the dual-labeled sample and obtain the full-spectrum count rate (cpm) of the dual-labeled sample. L+H and full-spectrum sample spectral index SIS L+H .

[0047] Specifically, the instrument switches the high-pressure setting to the dual-label high-pressure mode, which is most suitable for dual-labeled nuclide analysis. The dual-labeled sample is measured again, and the raw measurement data required for activity calculation is directly obtained and calculated from the energy spectrum: the full-spectrum count rate (cpm). L+H and full-spectrum sample spectral index SIS L+H .

[0048] S106, based on the full-spectrum count rate (cpm) of the dual-labeled sample. L+H Full-spectrum sample spectral index (SIS) L+H The counting efficiency E of low-energy nuclides L The counting efficiency E of high-energy nuclides H Low-energy nuclide sample spectral index (SIS) L and the spectral index value (SIS) of high-energy nuclides H Calculate the low-energy nuclide activity A of the dual-labeled sample.L and high-energy nuclide activity A H .

[0049] Specifically, based on the parameters obtained above, the low-energy nuclide activity A of the dual-labeled sample was analyzed. L and high-energy nuclide activity A H Solve the problem.

[0050] Through the above steps, this embodiment uses the pure spectrum index (ES_SIS) of an external standard source as the quenching indicator parameter and a stable and high-activity external gamma source to establish calibration curves for high- and low-energy nuclides, thereby decoupling the relationship between activity calculation and the sample's own activity and overcoming the shortcomings of the traditional full-spectrum DPM method. When solving for the quenching degree and efficiency of low- and high-energy nuclides, since ES_SIS is calculated based on a stable external gamma source signal, the entire solution process does not depend on the sample's own activity and count. This effectively solves the technical problem of poor accuracy of the quenching indicator parameter for low-activity samples or samples with a low proportion of nuclides in the prior art. Furthermore, this method has higher accuracy and a wider range of applicability. Because the calibration curve is established by a stable external gamma source, the accuracy of the parameters is guaranteed, resulting in higher accuracy of the activity calculation. At the same time, this method has no special requirements on the activity range and nuclide content ratio of the sample, greatly expanding the applicability of the dual-labeled nuclide analysis method. It is more operable in practical applications, improving user convenience.

[0051] In one embodiment, the full-spectrum count rate (cpm) of the dual-labeled sample L+H Full-spectrum sample spectral index (SIS) L+H The counting efficiency E of low-energy nuclides L The counting efficiency E of high-energy nuclides H Low-energy nuclide sample spectral index (SIS) L and the spectral index value (SIS) of high-energy nuclides H Calculate the low-energy nuclide activity A of the dual-labeled sample. L and high-energy nuclide activity A H The steps include:

[0052] Based on the full spectrum sample spectral index and the low-energy nuclide sample spectral index and high-energy nuclide sample spectral index obtained through the calibration curve, the full spectrum count rate is decomposed into the low-energy nuclide count rate and the high-energy nuclide count rate.

[0053] The count rates of the low-energy nuclides and the count rates of the high-energy nuclides obtained after decomposition are divided by the count efficiencies of the low-energy nuclides and the high-energy nuclides determined by the calibration curves, respectively, to complete the activity conversion. Finally, the low-energy nuclide activities and high-energy nuclide activities of the dual-labeled sample are calculated.

[0054] Specifically, in the field of liquid scintillation counting (LSC), the formula A = cpm / E is the basis for converting instrument measurement results into the true physical properties of the sample. Here, A represents activity (DPM, decays per minute), which is the actual number of nuclear decays of a radionuclide in the sample per unit time, and is the intrinsic physical quantity that is ultimately desired. cpm in the formula represents the count rate (Counts Per Minute), which is the number of pulse signals actually detected and recorded by the liquid scintillation spectrometer during the measurement time. Due to factors such as quenching effects, instrument background interference, and the inability of the detector to capture 100% of decays during the measurement process, cpm is always less than or equal to the true activity A of the sample. E represents the counting efficiency, which is the conversion factor connecting activity A and count rate cpm, defined as E = cpm / A. The physical meaning of efficiency E lies in quantifying the instrument's ability to convert each true nuclear decay in the sample into one effective count. This efficiency value is comprehensively affected by the type of nuclide, instrument settings, and the degree of quenching of the sample itself (absorption and quenching of scintillation light by the chemical environment).

[0055] In this embodiment, firstly, the full-spectrum sample spectral index SIS of the dual-labeled sample is calculated. L+H And the low-energy nuclide sample spectral index SIS obtained through calibration curves. L and high-energy nuclide sample spectral index SIS H The full-spectrum count rate (cpm) L+H The decomposition process utilizes the differences in spectral indices of different nuclides in the mixed energy spectrum to determine the cpm. L+H The count rate (cpm) of low-energy nuclides L ) and the count rate (cpm) of high-energy nuclides H The total count is decomposed into the count rate contribution of each nuclide by the proportion of the total count. Then, the count rates of the low-energy nuclides and the high-energy nuclides obtained after decomposition are divided by the accurate counting efficiency E determined by the ES_SIS correction curve. L and E H This division operation completes the conversion from count rate to true activity (according to the definition A=cpm / E), ultimately calculating the low-energy nuclide activity A of the dual-labeled sample. L and high-energy nuclide activity A H .

[0056] In this embodiment, the technical difficulties existing in the activity calculation step of the traditional full-spectrum DPM method are solved, greatly improving the accuracy and applicability of the final activity calculation. The traditional DPM method relies on the sample's own activity to determine the quenching indicator parameter, leading to limitations in the obtained efficiency E and pure spectrum index SIS. L / SIS HNot precise enough. This embodiment determines the precise E value by using an ES_SIS calibration curve that is unaffected by sample activity before activity decomposition. L E H SIS L and SIS H The values ​​ensure that the key parameters used for activity decomposition and transformation are highly accurate. Based on this, the calculation steps in this embodiment achieve the full-spectrum count rate (cpm) through the differences in SIS parameters. L+H The accurate decomposition is then performed, followed by dividing the decomposed count rate by the corrected accurate efficiency E. L and E H This enables accurate conversion from count rate to true activity. This activity decomposition process, which combines the advantages of ES_SIS, ensures high-accuracy activity determination even for samples with low activity or large differences in nuclide content ratios.

[0057] In one embodiment, the full-spectrum count rate (cpm) of the dual-labeled sample L+H Full-spectrum sample spectral index (SIS) L+H The counting efficiency E of low-energy nuclides L The counting efficiency E of high-energy nuclides H Low-energy nuclide sample spectral index (SIS) L and the spectral index value (SIS) of high-energy nuclides H Calculate the low-energy nuclide activity A of the dual-labeled sample. L and high-energy nuclide activity A H Specifically, it is calculated using the following formula:

[0058]

[0059] Among them, A L For low-energy nuclide activity, A H For high-energy nuclide activity, cpm L+H For full spectrum count rate, SIS L+H For full-spectrum sample spectral index, E L For the counting efficiency of low-energy nuclides, E H For high-energy nuclides counting efficiency, SIS L For low-energy nuclide sample spectral index, SIS H This is the spectral index of a high-energy nuclide sample.

[0060] Specifically, based on the full-spectrum sample spectral index SIS measured from dual-labeled samples. L+H And the low-energy nuclide pure spectrum index SIS obtained through the ES_SIS correction curve. L and high-energy nuclide pure spectrum index SIS H Construct the scaling factor (i.e., the scaling factor in the formula) and (Partial). These scaling factors are used to adjust the full-spectrum count rate (cpm) of the double-labeled sample. L+H The count rate (cpm) of decomposition into low-energy nuclides L and the count rate (cpm) of high-energy nuclides H Then, an activity conversion is performed: the count rates of low-energy nuclides and high-energy nuclides obtained after decomposition are divided by the accurate counting efficiency E determined by the ES_SIS calibration curve. L and E H This division operation strictly follows the activity definition A = cpm / E, ultimately calculating the low-energy nuclide activity A of the dual-labeled sample. L and high-energy nuclide activity A H This formula decomposes the total count rate by utilizing the difference in SIS values ​​and completes the final activity conversion using the corrected efficiency E, thereby accurately calculating the activities of low-energy and high-energy nuclides.

[0061] In this embodiment, the key parameter E in the formula L E H SIS L SIS H The cpm was determined using a stable and high-activity external gamma source (ES_SIS) calibration curve. This completely avoids the problems of poor accuracy and limited applicability caused by the traditional DPM method, which relies on the counting of low-activity samples to determine the quenching correction parameters. By substituting the accurate SIS parameters after ES_SIS correction into the activity decomposition formula, the cpm was ensured. L+H The decomposition process is more reliable. Ultimately, combined with precise efficiency E... L and E H The activity conversion process makes the activity solution results in this embodiment more accurate, and it does not have special requirements on the activity range and nuclide content ratio of the sample, thus having a wider range of applications.

[0062] In one embodiment, the step of measuring the standard quenching source and obtaining the external standard source spectral index of the standard quenching source specifically includes:

[0063] Under external γ source high pressure, the first energy spectrum of the standard quenching source is obtained by measuring the standard quenching source;

[0064] Under high pressure from an external γ source, the second energy spectrum of the standard quenching source is obtained by measuring the standard quenching source and the external γ source.

[0065] The energy spectrum of the external γ source is obtained by stripping the second energy spectrum from the first energy spectrum, and the external standard source index of the standard quenching source is calculated based on the energy spectrum of the standard quenching source.

[0066] Specifically, after the i-th series of quenching sources arrives at the measurement position, the first energy spectrum SPE1 is measured and obtained under the high pressure of the external γ source. After the SPE1 measurement is completed, the multichannel count stops and is cleared, indicating "external γ source rising". After the external γ source is in position, the standard quenching source + external γ source is measured under the high pressure of the external γ source to obtain the second energy spectrum SPE2. After the SPE2 measurement is completed, the multichannel count stops and is cleared, the external γ source is removed, and the standard source is measured under the high pressure of the dual-marker to obtain the third energy spectrum SPE3. The efficiency EFF of SPE3 is calculated. L / EFF H Spectral Index (SIS) L / SIS H Then, by stripping the spectrum (SPE2-SPE1), the energy spectrum is obtained, and the quenching indicator parameter ES_SIS of the external γ source is solved, thereby establishing a calibration curve. The specific steps are as follows: Figure 3 and Figure 4 .

[0067] In one embodiment, the step of measuring the dual-labeled sample and obtaining and calculating the external standard source index of the dual-labeled sample through external γ-source stripping specifically includes:

[0068] Under high pressure from an external γ source, the first energy spectrum of the double-labeled sample was obtained by measuring the double-labeled sample.

[0069] Under high pressure from an external γ source, the second energy spectrum of the dual-labeled sample is obtained by measuring the dual-labeled sample and the external γ source.

[0070] The energy spectrum of the external γ source is obtained by stripping the second energy spectrum of the dual-labeled sample to the first energy spectrum, and the external standard source index of the dual-labeled sample is calculated based on the energy spectrum of the dual-labeled sample.

[0071] Specifically, the method for external standard source spectral indexing with the same standard quenching source. First, prepare a double-labeled sample;

[0072] Under high pressure from an external γ source, the double-labeled sample was measured to obtain the first energy spectrum SPE1, and the quenching indicator parameter of SPE1 was calculated. Under high pressure from an external γ source, the double-labeled sample and the external γ source were measured to obtain the second energy spectrum SPE2. The spectrum was stripped and the spectral index ES_SIS of the external γ source was calculated. Then, the calibration curve was called to solve for the low-energy nuclide activity A of the double-labeled sample. L and high-energy nuclide activity A H ,like Figure 5 .

[0073] In the above embodiments, by measuring the standard quenching source under high pressure from an external γ source and performing a stripping operation between the second energy spectrum (SPE2) and the first energy spectrum (SPE1), the energy spectrum generated entirely by the external γ source can be purified. This stripping technique effectively eliminates the interference of the radionuclide signal of the standard source itself on the calculation of the quenching indicator parameter, ensuring that the calculated ES_SIS value accurately reflects the degree of chemical quenching of the sample. Secondly, this embodiment clarifies the dependent variable (EFF) for obtaining the calibration curve. L / EFF H and SIS L / SIS H The operational details of the process are detailed below. When calculating these key parameters, it is clearly stipulated that after the SPE2 measurement is completed, "multichannel stop counting and clearing, external γ source removal," and then switching to SPE3 measurement under dual-label high pressure. This rigorous operational procedure ensures that efficiency and the calculation of the pure spectrum index are performed under optimal dual-label measurement conditions without external γ source interference, guaranteeing the accuracy of the calibration curve. Finally, this process of stripping the spectrum and calculating ES_SIS is completely replicated in the measurement of unknown dual-labeled samples to obtain the quenching indicator parameters of the sample to be tested. Since ES_SIS depends on a stable and high-activity external γ source signal, the final obtained quenching indicator parameters do not depend on the activity or nuclide content ratio of the unknown sample, thus ensuring the high accuracy and wide applicability of the activity solution results of this invention from the source of data acquisition.

[0074] In one embodiment, the full-spectrum count rate of the dual-labeled sample is calculated by time normalization of the total count recorded over a preset measurement time period.

[0075] Specifically, the full-spectrum count rate cpm L+H The activity determination occurs during the activity calculation phase. Specifically, the measurement must be performed with the instrument's high-pressure setting switched to dual-label high pressure (i.e., the optimized LSA operating state). Within the set measurement time, the liquid scintillation spectrometer records the total counts emitted by the dual-labeled sample. To eliminate the influence of measurement time on the data, the total counts are subsequently calculated using time normalization. That is: cpm L+H =Total count / Measurement time (minutes)

[0076] In one embodiment, the full-spectrum sample index of the dual-labeled sample is calculated by the count ratio of a preset energy window or channel address.

[0077] The full-spectrum sample spectral index SIS L+H The same data was obtained when measuring the double-labeled sample under high pressure.

[0078] Specifically, LSA simultaneously acquires energy spectra (typically multichannel spectra) representing the mixed nuclides during the measurement. The SIS L+H It's not a simple total count, but rather a calculation done by dividing the energy spectrum into preset energy windows or channels, and then calculating the count ratio within those windows. For example, SIS L+H It could be the ratio of high-energy window counts to low-energy window counts. As a dimensionless characteristic parameter, SIS L+H It accurately characterizes the overall shape and photon energy distribution of the mixed energy spectrum and is a key parameter in the activity decomposition formula used to separate the contributions of low-energy and high-energy nuclides.

[0079] In the above embodiments, it is ensured that all directly measured input parameters required for activity decomposition calculations are accurate, objective, and comparable. Time normalization guarantees the accuracy of cpm. L+H The accuracy of the count, and the SIS calculated based on the energy spectrum ratio. L+H This stably reflects the true characteristics of the mixed energy spectrum. These two precise input data, combined with the high-precision E and SIS parameters after ES_SIS correction, jointly ensure the high accuracy of the final activity solution.

[0080] In one embodiment, the external γ source is 133 Ba.

[0081] Specifically, the external γ source is preferably... 133 Ba nuclide source. Using 133 Ba is chosen as an external gamma source primarily because of its stable and high activity. In the ES_SIS quenching correction method, selecting such a stable and high-activity external gamma source is crucial, as it ensures a stable, sufficient, and unaffected-by-sample-activity-independent gamma-ray signal during stripping operations on standard sources and unknown dual-labeled samples. This further guarantees that the calculated ES_SIS value accurately indicates the degree of sample quenching.

[0082] Furthermore, some embodiments of this application also provide an electronic device. The electronic device can be various forms of digital computer, such as laptop computers, desktop computers, workstations, personal digital assistants, servers, blade servers, mainframe computers, and so on.

[0083] The electronic device includes: one or more processors; and a memory storing computer program instructions that, when executed, cause the processor to perform the steps of the methods provided in any one or more of the above embodiments. Figure 6An exemplary structural diagram of the electronic device is disclosed. The electronic device includes one or more processors 1101, a memory 1102, and interfaces for connecting the components, including high-speed interfaces and low-speed interfaces. The components are interconnected via different buses and can be mounted on a common motherboard or otherwise installed as needed. The processors can process instructions executed within the electronic device, including instructions stored in or on memory to display graphical information of a GUI on an external input / output device (such as a display device coupled to the interface). In some other embodiments, multiple processors and / or multiple buses can be used with multiple memories and multiple memory modules, if desired. Similarly, multiple electronic devices can be connected, each providing some of the necessary operations. The components, their connections and relationships, and their functions shown herein are merely examples and are not intended to limit the implementation of the present application described and / or claimed herein.

[0084] The electronic device may further include an input device 1103 and an output device 1104. The processor 1101, memory 1102, input device 1103 and output device 1104 may be connected by a bus or other means, as shown in the figure, which is connected by a bus.

[0085] Input device 1103 can receive input numerical or character information, and generate key signal inputs related to user settings and function control of the electronic device, such as a touch screen, keypad, mouse, trackpad, touchpad, joystick, one or more mouse buttons, trackball, joystick, etc. Output device 1104 may include a display device, auxiliary lighting device (e.g., LED), and haptic feedback device (e.g., vibration motor). The display device may include, but is not limited to, a liquid crystal display, a light-emitting diode display, and a plasma display. In some embodiments, the display device may be a touch screen.

[0086] To provide interaction with the user, the electronic device can be a computer. The computer has: a display device (e.g., a cathode ray tube or LCD monitor) for displaying information to the user; and a keyboard and pointing device (e.g., a mouse) through which the user provides input to the computer. Other types of devices can also be used to provide interaction with the user; for example, feedback provided to the user can be any form of sensory feedback (e.g., visual feedback, auditory feedback); and input from the user can be received in any form (e.g., voice input or tactile input).

[0087] In this embodiment, a computer-readable medium stores a computer program / instructions that, when executed by a processor, implement the steps of the methods provided in any one or more of the above embodiments. This computer-readable medium may be included in the electronic device described in the above embodiments; or it may exist independently and not assembled into that device. The aforementioned computer-readable medium carries one or more computer-readable instructions.

[0088] The memory 1102 can serve as a non-transitory computer-readable storage medium, used to store non-transitory software programs, non-transitory computer-executable programs, and modules. The processor 1101 executes various functional applications and data processing of the server by running the non-transitory software programs, instructions, and modules stored in the memory 1102, thereby implementing the program instructions / modules corresponding to the methods provided in any one or more of the embodiments described above in this application.

[0089] The memory 1102 may include a program storage area and a data storage area. The program storage area may store the operating system and applications required for at least one function; the data storage area may store data created based on the use of the electronic device. Furthermore, the memory 1102 may include high-speed random access memory and may also include non-transitory memory, such as at least one disk storage device, flash memory device, or other non-transitory solid-state storage device. In some embodiments, the memory 1102 may optionally include memory remotely located relative to the processor 1101, and these remote memories can be connected to the electronic device via a network. Examples of such networks include, but are not limited to, the Internet, intranets, local area networks, mobile communication networks, and combinations thereof.

[0090] It should be noted that the computer-readable medium described in this application can be a computer-readable signal medium or a computer-readable storage medium, or any combination thereof. Computer-readable media can be, for example, but not limited to, electrical, magnetic, optical, electromagnetic, infrared, or semiconductor systems, apparatuses, or devices, or any combination thereof. More specific examples of computer-readable storage media may include, but are not limited to, electrical connections having one or more wires, portable computer disks, hard disks, random access memory, read-only memory, erasable programmable read-only memory, optical fibers, portable compact disk read-only memory, optical storage devices, magnetic storage devices, or any suitable combination thereof. In this application, a computer-readable medium can be any tangible medium containing or storing a program that can be used by or in conjunction with an instruction execution system, apparatus, or device.

[0091] Computer-readable media include permanent and non-permanent, removable and non-removable media, which can store information by any method or technology. Information can be computer-readable instructions, data structures, program modules, or other data. Examples of computer storage media include, but are not limited to, phase-change memory, static random access memory, dynamic random access memory, other types of random access memory, read-only memory, electrically erasable programmable read-only memory, flash memory or other memory technologies, read-only optical discs, digital versatile optical discs or other optical storage, magnetic tape, magnetic disk storage or other magnetic storage devices, or any other non-transfer medium that can be used to store information accessible by a computing device.

[0092] Computer program code for performing the operations of this application can be written in one or more programming languages ​​or a combination thereof, including object-oriented programming languages ​​such as Java, Smalltalk, and C++, and conventional procedural programming languages ​​such as C or similar languages. The program code can be executed entirely on the user's computer, partially on the user's computer, as a standalone software package, partially on the user's computer and partially on a remote computer, or entirely on a remote computer or server. In cases involving remote computers, the remote computer can be connected to the user's computer via any type of network—including local area networks (LANs) or wide area networks (WANs), or it can be connected to an external computer (e.g., via the Internet using an Internet service provider).

[0093] In the above embodiments, all or part of the implementation can be achieved through software, hardware, firmware, or any combination thereof. For example, it can be implemented using an application-specific integrated circuit (ASIC), a general-purpose computer, or any other similar hardware device. In some embodiments, the software program of this application can be executed by a processor to implement the above steps or functions. Similarly, the software program of this application (including related data structures) can be stored in a computer-readable recording medium, such as RAM memory, magnetic or optical drives, floppy disks, and similar devices. In addition, some steps or functions of this application can be implemented in hardware, for example, as circuitry that cooperates with a processor to perform the various steps or functions.

[0094] The computer program product provided in this application includes one or more computer programs / instructions. When executed by a processor, these computer programs / instructions generate, in whole or in part, the processes or functions described in this application. The computer may be a general-purpose computer, a special-purpose computer, a computer network, or other programmable device. The computer instructions may be stored in a computer-readable storage medium or transmitted from one computer-readable storage medium to another. For example, the computer instructions may be transmitted from one website, computer, server, or data center to another website, computer, server, or data center via wired (e.g., coaxial cable, fiber optic, digital subscriber line) or wireless (e.g., infrared, wireless, microwave, etc.) means. The computer-readable storage medium may be any available medium that a computer can access or a data storage device such as a server or data center that integrates one or more available media. The available medium may be a magnetic medium (e.g., floppy disk, hard disk, magnetic tape), an optical medium (e.g., DVD), or a semiconductor medium (e.g., solid-state drive), etc.

[0095] The flowcharts or block diagrams in the accompanying drawings illustrate the architecture, functionality, and operation of possible implementations of devices, methods, and computer program products according to various embodiments of this application. In this regard, each block in a flowchart or block diagram may represent a module, segment, or portion of code containing one or more executable instructions for implementing a specified logical function. It should also be noted that in some alternative implementations, the functions indicated in the blocks may occur in a different order than those indicated in the drawings. For example, two consecutively indicated blocks may actually be executed substantially in parallel, and they may sometimes be executed in reverse order, depending on the functions involved. It should also be noted that each block in the block diagrams and / or flowcharts, and combinations of blocks in the block diagrams and / or flowcharts, may be implemented using a dedicated hardware-specific system that performs the specified function or operation, or using a combination of dedicated hardware and computer instructions.

[0096] The scope of this application is defined by the appended claims rather than the foregoing description, and is therefore intended to encompass all variations falling within the meaning and scope of equivalents of the claims. No reference numerals in the claims should be construed as limiting the scope of the claims. Furthermore, it is clear that the word "comprising" does not exclude other units or steps, and the singular does not exclude the plural. Multiple units or devices recited in a device claim may also be implemented by a single unit or device in software or hardware. Terms such as "first," "second," etc., are used only for distinguishing descriptions and do not indicate any particular order, nor should they be construed as indicating or implying relative importance.

[0097] The above description is merely a specific embodiment of this application, but the scope of protection of this application is not limited thereto. Any variations or substitutions that can be easily made by those skilled in the art within the scope of the technology disclosed in this application should be included within the scope of protection of this application. Therefore, the scope of protection of this application should be determined by the scope of the claims, and the above embodiments should be regarded as exemplary and non-limiting.

Claims

1. A method of analysis of a dual-labeled nuclide, characterized by, The method is used for solving low-energy nuclide activity and high-energy nuclide activity in a liquid scintillation spectrometer, and the method comprises the following steps: Measuring a standard quenching source to obtain an ex-source spectrum index of the standard quenching source; Pairing data points are formed by taking the ex-source spectrum index of the standard quenching source as an independent variable and taking low-energy nuclide counting efficiency, high-energy nuclide counting efficiency, low-energy nuclide sample spectrum index and high-energy nuclide sample spectrum index of the standard quenching source as dependent variables; mathematical fitting is performed on the data points to establish a functional relationship between the independent variable and the dependent variable, and a correction curve is drawn; A double-labeled sample is measured under high pressure of an ex-gamma source, and an ex-source spectrum index of the double-labeled sample is obtained and calculated by ex-gamma source stripping spectrum; According to the ex-source spectrum index of the double-labeled sample and the correction curve, counting efficiency of the low-energy nuclide, counting efficiency of the high-energy nuclide, low-energy nuclide sample spectrum index and high-energy nuclide sample spectrum index of the double-labeled sample corresponding to the current quenching level are determined; The double-labeled sample is measured under double-labeled high pressure to obtain a full-spectrum counting rate and a full-spectrum sample spectrum index of the double-labeled sample; Based on the full-spectrum counting rate, the full-spectrum sample spectrum index, the counting efficiency of the low-energy nuclide, the counting efficiency of the high-energy nuclide, the low-energy nuclide sample spectrum index and the high-energy nuclide sample spectrum index of the double-labeled sample, the low-energy nuclide activity and the high-energy nuclide activity of the double-labeled sample are calculated.

2. The method of claim 1, wherein, The step of calculating the low-energy nuclide activity and the high-energy nuclide activity of the double-labeled sample based on the full-spectrum counting rate, the full-spectrum sample spectrum index, the counting efficiency of the low-energy nuclide, the counting efficiency of the high-energy nuclide, the low-energy nuclide sample spectrum index and the high-energy nuclide sample spectrum index of the double-labeled sample comprises: Based on the full-spectrum sample spectrum index and the low-energy nuclide sample spectrum index and the high-energy nuclide sample spectrum index obtained through the correction curve, the full-spectrum counting rate is decomposed into a low-energy nuclide counting rate and a high-energy nuclide counting rate; The low-energy nuclide counting rate and the high-energy nuclide counting rate obtained after decomposition are respectively divided by the counting efficiency of the low-energy nuclide and the counting efficiency of the high-energy nuclide determined through the correction curve to complete activity conversion, and finally the low-energy nuclide activity and the high-energy nuclide activity of the double-labeled sample are calculated.

3. The method of claim 1, wherein, The low-energy nuclide activity and the high-energy nuclide activity of the double-labeled sample are calculated based on the full-spectrum counting rate, the full-spectrum sample spectrum index, the counting efficiency of the low-energy nuclide, the counting efficiency of the high-energy nuclide, the low-energy nuclide sample spectrum index and the high-energy nuclide sample spectrum index of the double-labeled sample, and are specifically calculated through the following formula: where A L is the low-energy nuclide activity, A H is the high-energy nuclide activity, cpm L+H is the total spectrum count rate, SIS L+H is the total spectrum sample spectrum index, E L is the counting efficiency for the low-energy nuclide, E H is the counting efficiency for the high-energy nuclide, SIS L is the sample spectrum index for the low-energy nuclide, SIS H is the sample spectrum index for the high-energy nuclide.

4. The method of claim 1, wherein, The step of measuring the standard quenching source to obtain the ex-source spectrum index of the standard quenching source specifically comprises: The standard quenching source is measured under high pressure of an ex-gamma source to obtain a first energy spectrum of the standard quenching source; The standard quenching source and the ex-gamma source are measured under high pressure of the ex-gamma source to obtain a second energy spectrum of the standard quenching source; The energy spectrum of the ex-gamma source is obtained by stripping the second energy spectrum-first energy spectrum, and the ex-source spectrum index of the standard quenching source is calculated based on the energy spectrum of the standard quenching source.

5. The method of claim 1, wherein, The step of measuring the double-labeled sample, obtaining and calculating the ex-source spectrum index of the double-labeled sample through ex-gamma source stripping spectrum specifically comprises: measuring the double-labeled sample under high pressure of external gamma source to obtain a first energy spectrum of the double-labeled sample; measuring the double-labeled sample and the external gamma source under high pressure of external gamma source to obtain a second energy spectrum of the double-labeled sample; obtaining an energy spectrum of the external gamma source by stripping the second energy spectrum of the double-labeled sample minus the first energy spectrum of the double-labeled sample, and calculating the external gamma source spectrum index of the double-labeled sample based on the energy spectrum of the double-labeled sample.

6. The method of claim 1, wherein, The total spectrum count rate of the double-labeled sample is calculated by total counts recorded within a preset measurement time and time normalization.

7. The method of claim 1, wherein, The total spectrum sample spectrum index of the double-labeled sample is calculated by count ratio within a preset energy window or channel address.

8. The method of claim 1, wherein, The external gamma source is 133 Ba.

9. An electronic device, comprising: The electronic device comprises: one or more processors; and a memory storing computer program instructions which, when executed, cause the processor to perform the steps of the method of any one of claims 1 to 8.

10. A computer program product comprising computer programs / instructions, characterized in that, The computer program / instructions, when executed by the processor, implement the steps of the method of any one of claims 1 to 8.

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