A method for modeling liver cancer in tree shrews

By using a sequential intervention method combining DEN and 2-PCCA, the problems of long cycle and high mortality of DEN-only induction method were solved, and a highly efficient and stable tree shrew liver cancer model was constructed, which simulated the pathological process of human liver cancer and provided a clinically relevant animal model.

CN121796368BActive Publication Date: 2026-06-19YUNNAN YUNKE BIOTECHNOLOGY RES INST
View PDF 2 Cites 0 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
YUNNAN YUNKE BIOTECHNOLOGY RES INST
Filing Date
2026-03-12
Publication Date
2026-06-19

AI Technical Summary

Technical Problem

Existing DEN-based induction methods for constructing liver cancer models are characterized by long cycles, high mortality rates, and unstable tumorigenesis rates, making it difficult to simulate the pathological process of human liver cancer.

Method used

A sequential intervention method combining DEN and 2-PCCA was adopted. A tree shrew liver cancer model was established by intraperitoneal injection of DEN and gavage administration of 2-PCCA. The specific dosage and timing were as follows: DEN 50 mg/kg once a week, and 2-PCCA 20 mg/kg starting three days after DEN administration, for 11 consecutive weeks.

Benefits of technology

Within 11 weeks, a 100% tree shrew liver cancer model was successfully induced, with improved model stability and reproducibility. Serum AFP levels were significantly elevated, and liver function indicators were abnormal, mimicking the typical pathological process of human liver cancer and providing a clinically relevant animal model.

✦ Generated by Eureka AI based on patent content.

Smart Images

  • Figure CN121796368B_ABST
    Figure CN121796368B_ABST
Patent Text Reader

Abstract

This invention discloses a method for modeling liver cancer in tree shrews, belonging to the field of disease model construction technology. (1) Reagent preparation: Under sterile conditions, weigh the required N-nitrosodiethylamine, dissolve it in sterile ultrapure water, prepare a 10 mg / mL N-nitrosodiethylamine working solution, filter it with a 0.22 μm cell filter and set it aside; Under sterile conditions, weigh the required 2-PCCA, dissolve it in sterile ultrapure water, prepare a 10 mg / mL 2-PCCA working solution, filter it with a 0.22 μm cell filter and set it aside; (2) Administer the N-nitrosodiethylamine working solution prepared in step (1) to tree shrews via intraperitoneal injection at a dose of 50 mg / kg, once a week. Three days after the administration of N-nitrosodiethylamine, administer the 2-PCCA working solution to tree shrews via gavage at a dose of 20 mg / kg. The modeling is completed in 11 consecutive weeks. To address the shortcomings of existing animal models of liver cancer, this invention proposes for the first time that the combined use of DEN and 2-PCCA can establish a stable tree shrew liver cancer model.
Need to check novelty before this filing date? Find Prior Art

Citation Information

Patent Citations

  • Macaca mulatta hepatocellular carcinoma model, macaca mulatta hepatocellular carcinoma cell line and application of macaca mulatta hepatocellular carcinoma model and macaca mulatta hepatocellular carcinoma cell line

    CN105343897A

  • Macaca fascicularis liver cancer model and establishment method thereof

    CN105816447A