Anti-hypoxia composition containing saussurea involucrata culture and preparation method thereof

Abstract

The invention discloses an anti-hypoxia composition containing saussurea involucrata culture and a preparation method thereof, belongs to the technical field of food, and relates to functional food. The anti-hypoxia composition containing the saussurea involucrata culture comprises the following components in parts by weight: 150-250 parts of the saussurea involucrata culture, 0-25 parts of a magnesium element reagent, 0-1.5 parts of a zinc element reagent and 0-4 parts of an iron element reagent, the isomaltitol tablet is prepared from the following raw materials in parts by weight: 400-500 parts of isomaltitol, 2-6 parts of hydroxypropyl methyl cellulose, 0.1-2 parts of sucralose, 0.1-2 parts of sodium carboxymethyl cellulose, 2-6 parts of magnesium stearate and 4-10 parts of food-grade coating powder. The composition disclosed by the invention not only can assist in improving altitude stress symptoms and relieving adverse effects of hypoxia on cardiopulmonary tissues, but also has the advantages of safety, portability, stability and compliance, and is suitable for various crowds exposed to a plateau environment.

Description

Technical Field

[0001] This invention belongs to the field of food technology, and relates to functional foods, specifically to an anti-hypoxia composition containing snow lotus culture and its preparation method. Background Technology

[0002] With the increasing prevalence of high-altitude tourism, development in high-altitude areas, and scientific research activities, the impact of the high-altitude environment on human health has received growing attention. High-altitude regions possess unique geographical and climatic characteristics such as low pressure, hypoxia, large diurnal temperature variations, and strong ultraviolet radiation. Among these, low pressure and hypoxia are the core triggers for altitude sickness. Altitude sickness, also known as mountain sickness, is a series of physiological dysfunction symptoms caused by the body's incomplete adaptation to the hypoxic environment after entering a high-altitude environment above 3000 meters. Common symptoms include dizziness, headache, shortness of breath, fatigue, nausea, vomiting, and sleep disturbances. In severe cases, it can even induce critical illnesses such as high-altitude pulmonary edema and cerebral edema. This not only affects the travel experience of high-altitude travelers but also poses a potential threat to the health and work efficiency of groups such as those working long-term in high-altitude areas and border guards.

[0003] Currently, the main ways to cope with altitude sickness include three categories: advance acclimatization, physical protection, and the use of auxiliary products. Advance acclimatization takes a long time and is difficult to meet the needs of people traveling to high altitudes for short periods; physical protection (such as oxygen inhalation) is limited by equipment, lacks portability, and cannot provide continuous protection; existing auxiliary products are mostly pharmaceutical preparations or single-ingredient food products; among them, pharmaceutical preparations may have side effects, and single-ingredient products, due to their simple mechanism of action, are difficult to comprehensively alleviate altitude sickness symptoms and protect body tissues. Therefore, developing a safe, portable, and fully effective food composition adapted to the high-altitude environment has become an urgent need for the industry.

[0004] Snow lotus, a rare medicinal plant unique to the plateau, is rich in nutrients such as flavonoids, polysaccharides, and alkaloids. However, natural snow lotus is a Class II protected wild plant in China, protected by law and its illegal harvesting is prohibited. Furthermore, its natural resources are scarce, making it impossible to meet the needs of large-scale application. Patent CN 121058558 A discloses a technical solution for obtaining snow lotus cultures through artificial tissue culture technology. The types and contents of its effective components are similar to those of natural snow lotus, and its components are rich in selenium and chromium, offering advantages such as legal sourcing, controllable quality, and the potential for large-scale production. While there have been attempts to combine medicinal plants with other nutrients to prepare health foods, research on the specific needs of the body in the high-altitude environment is limited. The efficacy in alleviating the adverse effects of hypoxia on cardiopulmonary tissues is low, the preparation process has poor stability, and there is a significant market gap.

[0005] To address the aforementioned problems in existing technologies, a hypoxia-resistant composition containing snow lotus culture and its preparation method were developed. Summary of the Invention

[0006] The purpose of this invention is to provide a hypoxia-resistant composition containing snow lotus culture.

[0007] Another object of the present invention is a method for preparing an anti-hypoxia composition containing snow lotus culture.

[0008] To achieve the above objectives, the present invention discloses the following technical solutions: A hypoxia-resistant composition containing snow lotus culture, comprising the following components in parts by weight: 150-250 parts of snow lotus culture, 0-25 parts of magnesium reagent, 0-1.5 parts of zinc reagent. Iron reagent 0-4 parts, isomalt 400-500 parts, hydroxypropyl methylcellulose 2-6 parts Sucralose 0.1-2 parts, sodium carboxymethyl cellulose 0.1-2 parts, magnesium stearate 2-6 parts 4-10 parts of food-grade coating powder.

[0009] Furthermore, the hypoxia-resistant composition containing snow lotus culture comprises the following components in parts by weight: 175-220 parts of snow lotus culture, 15-25 parts of magnesium reagent, and 0.2-1.5 parts of zinc reagent. Iron element reagent 1-4 parts.

[0010] The hypoxia-resistant composition containing snow lotus culture comprises the following components in parts by weight: 175-200 portions of snow lotus culture, 17-22 portions of magnesium reagent, 0.5-1 portion of zinc reagent, and 1.5-3 portions of iron reagent.

[0011] The magnesium element reagent is one or more of the following: magnesium citrate, magnesium gluconate, magnesium L-threonate, magnesium vitamin C phosphate, magnesium sulfate, magnesium chloride, magnesium oxide, magnesium carbonate, and magnesium hydrogen phosphate.

[0012] The zinc element reagent is one or more of the following: zinc sulfate, zinc gluconate, zinc glycinate, zinc oxide, zinc lactate, zinc citrate, zinc chloride, zinc acetate, zinc carbonate, zinc carnosine, zinc pyridinecarboxylate, and zinc-enriched yeast.

[0013] The iron element reagent is one or more of the following: ferrous sulfate, ferrous gluconate, ferric ammonium citrate, ferrous fumarate, ferric citrate, ferrous lactate, ferric heme chloride, ferric pyrophosphate, ferric porphyrin, ferrous glycinate, reduced iron, ferric sodium ethylenediaminetetraacetate, carbonyl iron powder, ferrous carbonate, ferrous citrate, ferrous fumarate, ferrous succinate, heme iron, and electrolytic iron.

[0014] The food-grade coating powder is one or more of hydroxypropyl methylcellulose, povidone, talc, and polyethylene glycol.

[0015] Furthermore, the preparation method of the hypoxia-resistant composition containing snow lotus culture is a wet granulation and tableting method, and the preparation process includes the following steps: (1) Pretreatment: The snow lotus culture was crushed to ≥100 mesh; (2) Mixing and granulation: First, mix the zinc reagent with an equal amount of isomaltitol to obtain mixed powder A; Mixed powder A with an equal amount of isomaltitol to obtain mixed powder B; Mixed powder B is mixed with iron element reagent to obtain mixed powder C; Mixed powder C with an equal amount of isomaltitol yielded mixed powder D; The pulverized snow lotus culture, hydroxypropyl methylcellulose, mixed powder D, magnesium element reagent, sucralose, and the remaining isomaltitol were added to the mixing granulator in sequence and stirred for 15-20 minutes to obtain the mixture. Then, sodium carboxymethyl cellulose was prepared into an aqueous solution with a mass concentration of 1%-2% as a binder, added to the mixture, and then granulated to obtain wet granules. (3) Drying: The wet granules are spread evenly in a fluidized bed dryer to dry, and granules are obtained; (4) Mixing: Add the granules and magnesium stearate to a mixer, mix well, compress into tablets, and coat to obtain the finished product.

[0016] The mixing granulator has a stirring paddle speed of 15 Hz to 25 Hz, a blade speed of 40 Hz to 50 Hz, and a mixing time of 15 to 20 minutes.

[0017] The dryer has a motor frequency of 30-40 Hz, an inlet air temperature of 50-70°C, an outlet air temperature of 45-50°C, and dries the particles to a moisture content of 2%-4%.

[0018] The composition of this invention can protect the cardiopulmonary system and reduce the damage to cardiopulmonary tissues caused by the low pressure and hypoxia of high altitude environments; it can prevent and alleviate discomfort symptoms such as dizziness, headache, shortness of breath, and fatigue that occur in high-altitude environments. It is suitable for high-altitude travelers, long-term workers in high-altitude areas, and other people exposed to high-altitude environments, and helps maintain the body's normal physiological state in high-altitude environments.

[0019] This invention analyzes the special needs of the body in a high-altitude environment and uses snow lotus culture, magnesium, zinc and iron as the main anti-altitude reaction components to prepare an anti-hypoxia composition. By utilizing the synergistic effect between the components and controlling key parameters such as particle size and moisture content, the dissolution rate and stability of the effective components in the finished product are improved.

[0020] The active ingredients of snow lotus include flavonoids, polysaccharides, alkaloids, etc., which have the effects of resisting hypoxia, anti-fatigue, anti-inflammation and enhancing the body's tolerance. They can help the body maintain normal physiological functions in hypoxic environments. Snow lotus culture is also rich in selenium and chromium.

[0021] Mineral elements are essential for maintaining normal physiological functions in the human body. In the hypoxic environment of high altitudes, the body's need for certain mineral elements increases significantly. Appropriate supplementation of mineral elements is crucial for alleviating altitude sickness and protecting body tissues. Magnesium, an essential macro-element, participates in various physiological processes such as energy metabolism, myocardial contraction, and nerve conduction. It can improve myocardial oxygen supply, alleviate myocardial fatigue under hypoxic conditions, regulate electrolyte balance, and reduce dizziness and fatigue symptoms in high-altitude environments. Zinc, a trace element, plays an important role in regulating immune system function and repairing cell damage. It can enhance the body's tolerance to hypoxic environments, reduce oxidative damage to cardiopulmonary tissues caused by low-pressure hypoxia, and promote the activity of energy metabolism enzymes, thus alleviating altitude fatigue. Iron, an important trace element, participates in hemoglobin synthesis and is a core carrier of oxygen transport. In the hypoxic environment of high altitudes, it can improve blood oxygen-carrying efficiency, ensure organ oxygen supply, alleviate altitude sickness symptoms such as palpitations and dizziness, and help the body adapt to the hypoxic environment.

[0022] This invention combines snow lotus culture with magnesium, zinc, and iron to synergistically enhance the body's tolerance to hypoxia. Snow lotus culture has anti-hypoxia, anti-fatigue, anti-inflammatory, and body-enhancing effects, helping the body maintain normal physiological functions in hypoxic environments. Magnesium regulates electrolyte balance and improves myocardial function, zinc repairs cell damage and enhances immunity, and iron helps carry oxygen and ensures organ oxygen supply. Rational supplementation is of great significance in alleviating altitude sickness and protecting body tissues. The dosage of the three elements has been verified through multiple experiments to meet the special needs of the body in high-altitude environments.

[0023] Isomalt, as a sweetener and filler, is low in calories and high in sweetness, helping to maintain relatively stable blood sugar levels. It also has good flowability and compressibility, making it suitable for tableting processes. Hydroxypropyl methylcellulose and sodium carboxymethyl cellulose, as binders, have moderate viscosity, ensuring granule formation and tablet hardness, preventing granules from being too hard or too loose. Sucralose, a high-intensity sweetener, can adjust the product's taste. Magnesium stearate, as a lubricant, can reduce sticking and impaction during tableting, ensuring uniformity in tablet appearance and weight. The coating powder used is a film coating powder, mainly composed of hydroxypropyl methylcellulose, povidone, and talc, which can improve tablet stability, mask off-flavors, and enhance portability.

[0024] Compared with the prior art, the present invention has the following significant advantages: 1. Strong synergistic effects and targeted benefits: With snow lotus culture as the core, combined with iron, magnesium and zinc elements, the three work synergistically. Snow lotus culture exerts anti-hypoxia and anti-fatigue effects, magnesium regulates myocardial function and electrolyte balance, and zinc repairs tissue damage and enhances immunity. Compared with single-ingredient products, it can more comprehensively help improve altitude sickness symptoms, reduce the adverse effects of hypoxia on cardiopulmonary tissues, and meet the multiple needs of the body in high-altitude environments.

[0025] 2. Raw materials are safe and compliant, and the source is controllable: Snow lotus culture is used to replace natural snow lotus, which avoids the legal risks of protecting wild snow lotus. The source is stable and the quality is controllable. All raw materials are selected with food grade or higher purity, the amount of added mineral elements meets food standards, there are no drug side effects, and the long-term consumption is highly safe.

[0026] 3. Optimized preparation process and excellent product stability: By refining key parameters such as particle size, mixing speed, and granulation temperature, the wet granulation and tableting process is optimized, which not only ensures the dissolution rate of the active ingredients but also avoids component degradation. The resulting tablets have suitable hardness, rapid disintegration, and stable moisture content. After coating, they are highly portable and have a long shelf life, meeting the storage and use needs of high-altitude travel.

[0027] 4. Wide range of applications and strong practicality: The product is in tablet form, which is convenient to take and has a precise dosage. It does not require special storage conditions and can be widely used by various groups of people exposed to high-altitude environments. It not only solves the emergency needs of short-term travelers with altitude sickness, but also meets the daily auxiliary needs of people who live in high-altitude areas for a long time.

[0028] In summary, the composition of this invention can not only help improve the symptoms of altitude sickness and reduce the adverse effects of hypoxia on cardiopulmonary tissues, but also has the advantages of being safe, portable, stable, and compliant, making it suitable for all types of people exposed to high-altitude environments. Attached Figure Description

[0029] Figure 1 Effects of hypoxia-resistant compositions containing snow lotus culture on body weight in rats under hypoxic conditions. Detailed Implementation Example

[0030] This embodiment provides an anti-hypoxia composition containing snow lotus culture, comprising the following raw materials in parts by weight: 250 parts snow lotus culture, 25 parts magnesium, 1.5 parts zinc, 4 parts iron, 400 parts isomaltitol, 2 parts hydroxypropyl methylcellulose, 0.1 parts sucralose, 0.1 parts sodium carboxymethyl cellulose, 2 parts magnesium stearate, and 4 parts food-grade coating powder. The corresponding reagents are food-grade or higher zinc acetate, magnesium citrate, and heme iron, with the reagent dosage calculated according to elemental mass conversion. The food-grade coating powder is a hydroxypropyl methylcellulose type film coating powder. Example

[0031] The difference between this embodiment and Embodiment 1 is that the reagents selected for each component are different, but the weight parts of each component are the same.

[0032] This embodiment provides a food composition for combating altitude sickness containing snow lotus culture, comprising the following raw materials in parts by weight: 250 parts snow lotus culture, 25 parts magnesium, 1.5 parts zinc, 4 parts iron, 400 parts isomaltitol, 2 parts hydroxypropyl methylcellulose, 0.1 parts sucralose, 0.1 parts sodium carboxymethyl cellulose, 2 parts magnesium stearate, and 4 parts food-grade coating powder. The corresponding reagents are food-grade or higher zinc sulfate, magnesium sulfate, and ferrous sulfate. The reagent dosage is calculated based on the purity and element content of each reagent to ensure that the actual weight of the elements meets the standards. The food-grade coating powder is a hydroxypropyl methylcellulose type film coating powder. Example

[0033] The difference between this embodiment and Embodiment 1 is that this embodiment selects the advantageous reagents and the advantageous ratios of each component.

[0034] This embodiment provides a food composition for combating altitude sickness containing snow lotus culture, comprising the following raw materials in parts by weight: 220 parts snow lotus culture, 25 parts magnesium, 1.5 parts zinc, 4 parts iron, 400 parts isomaltitol, 2 parts hydroxypropyl methylcellulose, 0.1 parts sucralose, 0.1 parts sodium carboxymethyl cellulose, 2 parts magnesium stearate, and 4 parts food-grade coating powder. The corresponding reagents are food-grade or higher zinc gluconate, magnesium L-threonate, and ferrous glycinate. The reagent dosage is calculated based on the purity and element content of each reagent to ensure that the actual weight of the elements meets the standards. The food-grade coating powder is a hydroxypropyl methylcellulose type film coating powder. Example

[0035] The difference between this embodiment and Embodiment 1 is that this embodiment uses the superior reagents and a better ratio for each component.

[0036] This embodiment provides a food composition for combating altitude sickness containing snow lotus culture, comprising the following raw materials in parts by weight: 190 parts snow lotus culture, 20 parts magnesium, 0.8 parts zinc, 2.5 parts iron, 400 parts isomaltitol, 2 parts hydroxypropyl methylcellulose, 0.1 parts sucralose, 0.1 parts sodium carboxymethyl cellulose, 2 parts magnesium stearate, and 4 parts food-grade coating powder. The corresponding reagents are food-grade or higher carnosine zinc, magnesium vitamin C phosphate, and ferric heme chloride. The reagent dosage is calculated based on the purity and element content of each reagent to ensure that the actual weight of the elements meets the standards. The food-grade coating powder is a hydroxypropyl methylcellulose type film coating powder. Example

[0037] The difference between this embodiment and embodiment 4 is that this embodiment does not add snow lotus culture.

[0038] This embodiment provides a food composition for combating altitude sickness, comprising the following raw materials in parts by weight: 20 parts magnesium, 0.8 parts zinc, 2.5 parts iron, 400 parts isomaltitol, 2 parts hydroxypropyl methylcellulose, 0.1 parts sucralose, 0.1 parts sodium carboxymethyl cellulose, 2 parts magnesium stearate, and 4 parts food-grade coating powder. The corresponding reagents are food-grade or higher carnosine zinc, magnesium vitamin C phosphate, and ferric heme chloride. The reagent dosage is calculated based on the purity and element content of each reagent to ensure that the actual weight of the elements meets the standards. The food-grade coating powder is a hydroxypropyl methylcellulose type film coating powder. Example

[0039] The difference between this embodiment and embodiment 4 is that iron is not added in this embodiment.

[0040] This embodiment provides a food composition for combating altitude sickness containing snow lotus culture, comprising the following raw materials in parts by weight: 190 parts snow lotus culture, 20 parts magnesium, 0.8 parts zinc, 400 parts isomalt, 2 parts hydroxypropyl methylcellulose, 0.1 parts sucralose, 0.1 parts sodium carboxymethyl cellulose, 2 parts magnesium stearate, and 4 parts food-grade coating powder. The corresponding reagents are food-grade or higher carnosine zinc and magnesium vitamin C phosphate. The reagent dosage is calculated based on the purity and element content of each reagent to ensure that the actual weight of the elements meets the standards. The food-grade coating powder is a hydroxypropyl methylcellulose type film coating powder. Example

[0041] The difference between this embodiment and embodiment 4 is that the reagents selected for each component are the same and the weight proportions are the same, and no zinc element is added in this embodiment.

[0042] This embodiment provides a food composition for combating altitude sickness containing snow lotus culture, comprising the following raw materials in parts by weight: 190 parts snow lotus culture, 20 parts magnesium, 2.5 parts iron, 400 parts isomalt, 2 parts hydroxypropyl methylcellulose, 0.1 parts sucralose, 0.1 parts sodium carboxymethyl cellulose, 2 parts magnesium stearate, and 4 parts food-grade coating powder. The corresponding reagents are food-grade or higher magnesium vitamin C phosphate and ferric heme chloride. The reagent dosage is calculated based on the purity and element content of each reagent to ensure that the actual weight of the elements meets the standards. The food-grade coating powder is a hydroxypropyl methylcellulose type film coating powder. Example

[0043] The difference between this embodiment and embodiment 4 is that magnesium is not added in this embodiment.

[0044] This embodiment provides a food composition for combating altitude sickness containing snow lotus culture, comprising the following raw materials in parts by weight: 190 parts snow lotus culture, 0.8 parts zinc, 2.5 parts iron, 400 parts isomalt, 2 parts hydroxypropyl methylcellulose, 0.1 parts sucralose, 0.1 parts sodium carboxymethyl cellulose, 2 parts magnesium stearate, and 4 parts food-grade coating powder. The corresponding reagents are food-grade or higher carnosine zinc and ferric heme chloride. The reagent dosage is calculated based on the purity and element content of each reagent to ensure that the actual weight of the elements meets the standards. The food-grade coating powder is a hydroxypropyl methylcellulose type film coating powder.

[0045] To facilitate preparation and use, all reagents in the examples are food-grade or higher. Only one reagent is selected for preparation. For example, in Example 1, food-grade zinc acetate is selected as the zinc element reagent. In actual addition, multiple reagents can be used.

[0046] Preparation method The above-described Examples 1-8 were prepared using a wet granulation and tableting method. The specific steps are as follows: 1. Pretreatment: Grind the snow lotus culture to ≥100 mesh and set aside; 2. Mixing and Granulation: First, mix the zinc reagent with an equal amount of isomaltitol to obtain mixed powder A; mix mixed powder A with an equal amount of isomaltitol to obtain mixed powder B; mix mixed powder B with the iron reagent to obtain mixed powder C; mix mixed powder C with an equal amount of isomaltitol to obtain mixed powder D; then, add the pulverized snow lotus culture, hydroxypropyl methylcellulose, mixed powder D, magnesium reagent, sucralose, and the remaining isomaltitol to the mixing granulator in sequence. Set the stirring paddle speed to 20 Hz and the blade speed to 45 Hz, and mix for 18 minutes. (Example 5 does not add snow lotus culture); prepare a 1% (w / w) aqueous solution of sodium carboxymethyl cellulose as a binder and add it to the binder container. First, turn on the mixing granulator with the stirring paddle speed at 20 Hz and the blade speed at 45 Hz, then turn on the peristaltic pump speed at 60 Hz, add all the binder to the material, and granulate for 15 minutes. Wet granules E are obtained.

[0047] 3. Drying: Spread the wet granules E evenly in a fluidized bed dryer, set the motor frequency to 30HZ~40HZ, the inlet air temperature to 50~70℃, and the outlet air temperature to 45~50℃, and dry until the moisture content of the granules is 2%~4%. After granulation through a 16-mesh sieve, granules F are obtained. Strict control of moisture and temperature can avoid degradation of active ingredients, while ensuring the flowability of the granules and their suitability for tableting.

[0048] 4. Mixing: Add granules F and magnesium stearate to a mixer and mix for 5 minutes at a speed of 30 Hz.

[0049] 5. Tableting: A rotary tablet press is used for tableting. The tablet press speed is 15 rpm, and the tablet weight is controlled at 800 mg ±6% with a tablet hardness of 10~18 kg / mm2.

[0050] 6. Coating: The tablets obtained from compression are put into a coating machine. A 10% coating solution is prepared using food-grade coating powder and 50% ethanol. The air inlet temperature is adjusted to 40-60℃, the tablet bed temperature is controlled at 30-45℃, and the rotation speed of the pan is 6-14 r / min. Coating is carried out until the tablet weight gain is 0.5%-5%. After cooling to room temperature, the tablets are packaged to obtain the finished product. Endothelin-1: ET-1;

[0051] Nitric oxide: NO; Superoxide dismutase (SOD); Total antioxidants: TAC; Malondialdehyde (MDA) Total protein: TP; Albumin: ALB; Triglycerides: TG; Total cholesterol: TC; High-density lipoprotein cholesterol: HDL-C; Low-density lipoprotein cholesterol: LDL-C; Alanine aminotransferase (ALT); Total bilirubin: TBIL; Aspartate aminotransferase (AST); Serum blood urea nitrogen: BUN; Creatinine: Cr; Uric acid: UA; Lactate dehydrogenase: LDH; Creatine kinase: CK; α-Hydroxybutyrate dehydrogenase: α-HBDH The anti-hypoxia composition containing snow lotus culture prepared above was subjected to relevant efficacy tests, including tests on body weight, red blood cell index, liver function, kidney function, and cardiac function under long-term decompression and hypoxia environment.

[0052] The comparative examples used in the following tests were acetazolamide, which significantly improved the hypoxia tolerance of animals and reduced the mortality rate of acute hypoxia, and Rhodiola rosea powder, which were comparative examples 1 and 2. Comparative example 1 was acetazolamide and comparative example 2 was Rhodiola rosea powder.

[0053] Healthy male Wistar rats weighing 180-220 g were selected and, after 3 days of acclimatization, randomly divided into 12 groups of 9 rats each. All rats were administered the drug via gavage. Specific grouping and administration protocols are as follows: Hypoxia model group normoxic blank control group Examples 1-8 are experimental groups 1-8, with an oral gavage dose of 0.34633 g / kg of the composition of Examples 1-8; Comparative Example 1 (0.11083 g / kg acetazolamide administered by gavage), Comparative group 2 (0.53570 g / kg Rhodiola rosea powder administered by gavage); Examples 1-8 and Comparative Examples 1-2 were prepared into a paste / solution using an appropriate amount of purified water and administered by gavage. The hypoxia model group and the normoxic blank control group were given an equal volume of purified water by gavage. The administration schedule was once daily before entering the chamber. One hour after administration, animals from all groups requiring hypoxia exposure were simultaneously placed in the hypobaric chamber (a multi-factor composite environment simulation medical scientific experimental chamber). After sealing the chamber door, decompression was initiated at a rate of 10 m / s, raising the altitude to 5500 m and maintaining this altitude for 8 hours. The altitude was then lowered to normal at a rate of 10 m / s, the chamber door was opened, and the animals were removed and reared as usual. After 8 weeks of this intermittent hypoxia exposure, the rats were weighed, anesthetized by intraperitoneal injection of sodium pentobarbital, and blood was collected via the abdominal aorta for later use. One tube of EDTA-Ca... 2+ Anticoagulation was used for routine blood tests. One tube was not anticoagulated. The tubes were centrifuged at 4°C, and the supernatant was collected and stored at -20°C. This supernatant was used to detect blood glucose, endothelin-1 (ET-1), nitric oxide (NO), oxidative stress-related superoxide dismutase (SOD), total antioxidants (TAC), malondialdehyde (MDA), and total protein (TP), albumin (ALB), triglycerides (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), alanine aminotransferase (ALT), total bilirubin (TBIL), aspartate aminotransferase (AST), serum blood urea nitrogen (BUN), creatinine (Cr), uric acid (UA), lactate dehydrogenase (LDH), creatine kinase (CK), and α-hydroxybutyrate dehydrogenase (α-HBDH). A normoxic control group was used, without entering a hypobaric chamber; other procedures were the same as the study group. The metabolic and organ functions of rats after 8 weeks of chronic hypoxia were assessed.

[0054] Weight measurement Changes in body weight are a comprehensive reflection of the body's energy metabolism, tissue damage, and physiological state under hypoxic conditions. For example... Figure 1 The figures show the weight changes of rats in the blank group, hypoxia model group, Examples 1-8, and Comparative Examples 1 and 2 over 8 weeks. The weight changes in the hypoxia model group indicate that a low-pressure, low-oxygen environment significantly inhibits weight gain in rats, reflecting metabolic disorders and impaired physiological function. Examples 1-8: The weight of each experimental group increased continuously over time, and the increase was significantly greater than that of the hypoxia model group. The food compositions containing snow lotus culture of this invention (especially the advantageous formulations in Examples 1, 3, and 4) can significantly improve the inhibition of weight gain in rats under low-pressure, low-oxygen conditions.

[0055] Endothelial function test Because NO is chemically reactive, it is rapidly converted into nitrite (NO2⁻) and nitrate (NO3⁻) after metabolism in the body, and NO2⁻ can be further converted into NO3⁻. Therefore, nitrate reductase is used to specifically reduce NO3⁻ to NO2⁻. The concentration is reflected by the color intensity. Following the kit instructions, the absorbance of each tube was measured at a wavelength of 550 nm, and the NO content in the sample was calculated using the formula. Samples, standards, and HRP-labeled detection antibodies were added sequentially to microwells pre-coated with rat ET-1 capture antibody. After incubation and thorough washing, the samples were developed with the substrate TMB. TMB produced a blue product under the catalysis of peroxidase, which turned yellow after acid treatment. The color intensity was positively correlated with the ET-1 content in the sample. The absorbance (OD value) was measured at 450 nm using an ELISA reader. A linear regression curve was plotted with the standard concentration on the x-axis and the corresponding OD value on the y-axis. The concentration of each sample was calculated according to the curve equation.

[0056] As shown in Table 1, the results indicate that a hypoxic environment significantly disrupts endothelial function in rats. Compared with the normoxic control group, the hypoxic model group exhibited increased ET-1 levels and abnormally fluctuating NO levels, suggesting an imbalance in vasoconstriction and vasodilation, and significant endothelial damage. The composition of this invention improved this disordered state to varying degrees in all groups. Example 4 showed the best efficacy, with ET-1 levels closest to the normoxic control group and NO levels reaching 15.60 ± 3.00 μmol / L, consistent with its optimal weight gain, confirming its protective effect on the overall physiological state of the body. Examples 1-3 verified the scientifically stable formulation, demonstrating that even with the use of non-dominant reagents, it could still significantly regulate endothelial function. Examples 5-8 showed that snow lotus culture is the core component, with magnesium, zinc, and iron working synergistically, and the magnesium-deficient group showing the weakest effect. The protective effect of this composition on endothelial function is superior to that of acetazolamide and Rhodiola rosea powder, and it is safer as a food composition, with no potential drug side effects.

[0057] Table 1

[0058]

[0059] Oxidative stress index measurement MDA assay uses the condensation reaction of thiobarbiturate (TBA), a lipid peroxidation degradation product, which can form a red product with TBA, with a maximum absorption peak at 532 nm. The assay is performed according to the instructions and the content is calculated using the absorbance formula. SOD assay utilizes the xanthine-xanthine oxidase reaction system to generate superoxide anion radicals (O2⁻·), which oxidize hydroxylamine to nitrite, resulting in a purple-red color. SOD in the sample specifically inhibits this reaction, reducing nitrite formation and causing the absorbance of the assay tube to be lower than that of the control tube. The absorbance is measured at 550 nm, and the activity is calculated using the formula. Total antioxidant capacity (T-AOC) assay is based on the body's antioxidant substances reducing Fe³⁺ to Fe²⁺. Fe²⁺ forms a stable complex with phenanthrene-like substances. The absorbance is measured at 520 nm, and the antioxidant capacity is calculated according to the kit instructions and formula.

[0060] As shown in Table 2, the hypoxia model group exhibited significantly reduced SOD activity and increased MDA content, indicating that hypoxia induced oxidative stress damage. Example 4 showed the best performance, with SOD activity closest to the normoxic control group, the lowest MDA content, and the highest T-AOC, demonstrating the strongest antioxidant capacity, consistent with the previous results showing optimal body weight and endothelial function. The indicators of Example 3 were superior to those of Examples 1 and 2; Example 5 showed a weak antioxidant effect, confirming the core role of the snow lotus culture; among Examples 6-8, the magnesium-deficient group (Example 8) showed the worst effect, followed by the zinc-deficient group (Example 7), confirming the synergistic effect mechanism of magnesium, zinc, iron, and snow lotus culture. The antioxidant indicators of most groups (Examples 3, 4, and 6) of this composition were significantly better than those of Comparative Examples 1 and 2, and can alleviate high-altitude hypoxia damage by enhancing the body's antioxidant capacity.

[0061] Table 2

[0062] Blood biochemistry assay Using a fully automated blood biochemistry analyzer, the following parameters were measured: blood glucose, TP, ALB, TG, TC, HDL-C, LDL-C, ALT, TBIL, AST, BUN, Cr, UA, LDH, CK, and α-HBDH.

[0063] As shown in Table 3, the results indicate that the hypoxic environment significantly disrupted the homeostasis of glucose, lipid, and protein metabolism in rats. Compared with the normoxic control group, the hypoxic model group showed significantly lower blood glucose, decreased TP and ALB levels, and abnormally lower TG metabolism, TC, and HDL-C levels, suggesting an imbalance between energy metabolism and nutrient reserves and significant metabolic damage under hypoxic conditions. The compositions of this invention improved this metabolic disorder to varying degrees in all groups. Example 4 showed the best efficacy, with blood glucose, TP, and ALB levels closest to the normoxic control group, and TG, TC, and HDL-C levels restored to levels not significantly different from the normoxic control group, reflecting the comprehensive improvement in metabolic indicators and confirming the synergistic protective effect on glucose, lipid, and protein metabolism. Examples 1-3 verified the scientific validity and stability of the composition ratio of this invention; even with non-dominant reagent combinations, it can still significantly regulate glucose, lipid, and protein metabolism and effectively improve hypoxia-induced metabolic abnormalities. Examples 5-8 show that snow lotus culture is the core component for exerting metabolic protection. Mineral elements such as magnesium, zinc, and iron work synergistically with snow lotus culture to maintain metabolic homeostasis. Among them, the magnesium-deficient group (Example 5) showed the weakest metabolic improvement effect, further confirming the key role of magnesium in synergistic enhancement.

[0064] The composition of this invention has a protective effect on metabolic homeostasis and is safer as a food composition, with no potential drug side effects, and has significant advantages in metabolic regulation applications under hypoxic conditions.

[0065] Table 3: Effects of depressurization and hypoxia exposure on metabolic function indicators in rats for 8 weeks (n=9) The results in Tables 4, 5, and 6 show that a low-pressure, low-oxygen environment can significantly induce multi-organ dysfunction in rats. Compared with the normoxic control group, in the hypoxia model group: liver function: ALT, AST, and BIL levels were significantly increased, indicating hepatocyte damage and bilirubin metabolism disorder; kidney function: BUN, Cr, and UA levels were significantly increased, indicating impaired glomerular filtration function and uric acid excretion disorder; myocardial function: LDH, CK, and α-HBDH levels were abnormally decreased, indicating myocardial energy metabolism pathway disorder and cellular homeostasis imbalance.

[0066] These changes collectively indicate that low-pressure, low-oxygen environments can trigger synergistic damage to multiple organs, including the liver, kidneys, and myocardium.

[0067] All groups of the compositions of this invention can improve hypoxia-induced multi-organ dysfunction to varying degrees. Among them, Example 4 has the best efficacy, with its liver function indicators, kidney function indicators, and myocardial enzyme spectrum indicators being closest to those of the normoxic control group, confirming that it has the strongest synergistic protective effect on multi-organ function.

[0068] Examples 1-3 verified the scientific nature and stability of the composition ratio of the present invention. Even when using a non-dominant reagent combination, it can still significantly improve the levels of damage markers in the liver, kidneys, and myocardium, and effectively alleviate hypoxia-induced multi-organ dysfunction.

[0069] The results of Examples 5-8 show that snow lotus culture is the core component that plays a multi-organ protective role. Mineral elements such as magnesium, zinc, and iron work synergistically with snow lotus culture to maintain the functional homeostasis of the liver, kidney, and myocardium. Among them, the organ function improvement effect was weakest in the snow lotus culture deficiency group (Example 5) and the magnesium deficiency group (Example 8), which further confirms the synergistic effect mechanism of the core component and mineral elements.

[0070] The composition of this invention has a protective effect on multiple organ functions and is safer as a food composition, with no potential drug side effects. It has outstanding advantages in the application of multi-organ protection in hypoxic environments.

[0071] Table 4: Effects of depressurization and hypoxia exposure on liver function indicators in rats for 8 weeks (n=9) Table 5: Effects of depressurization and hypoxia exposure on renal function indicators in rats for 8 weeks (n=9) Table 6: Effects of depressurization and hypoxia exposure on cardiac function parameters in rats for 8 weeks (n=9) This embodiment confirms that the hypoxia-resistant composition containing snow lotus culture can significantly improve physiological damage in rats under depressurization and hypoxia conditions. This composition can alleviate hypoxia-induced inhibition of weight gain, regulate the ET-1 / NO balance to protect endothelial function, enhance SOD activity, reduce MDA content to exert antioxidant effects, maintain glycolipid-protein metabolic homeostasis, and improve the function of multiple organs including the liver, kidneys, and myocardium. Snow lotus culture exhibits synergistic effects with magnesium, zinc, and iron; the optimal ratio shows the best efficacy. Furthermore, as a food composition, it has high safety and no drug side effects, demonstrating promising application prospects in the field of hypoxia / altitude sickness.

[0072] The technical solutions provided by the embodiments of this disclosure have been described in detail above. Specific examples have been used in this document to illustrate the principles and implementation methods of the embodiments of this disclosure. The descriptions of the embodiments above are only for helping to understand the principles of the embodiments of this disclosure. At the same time, for those skilled in the art, there will be changes in the specific implementation methods and application scope based on the embodiments of this disclosure. Therefore, the content of this specification should not be construed as a limitation of this disclosure.

Claims

1. A hypoxia-resistant composition containing snow lotus culture, characterized in that, The components include the following parts by weight: 150-250 parts of snow lotus culture, 0-25 parts of magnesium reagent, 0-1.5 parts of zinc reagent. Iron reagent 0-4 parts, isomalt 400-500 parts, hydroxypropyl methylcellulose 2-6 parts Sucralose 0.1-2 parts, sodium carboxymethyl cellulose 0.1-2 parts, magnesium stearate 2-6 parts 4-10 parts of food-grade coating powder.

2. The hypoxia-resistant composition containing snow lotus culture according to claim 1, characterized in that, The hypoxia-resistant composition containing snow lotus culture comprises the following components in parts by weight: 175-220 parts of snow lotus culture, 15-25 parts of magnesium reagent, and 0.2-1.5 parts of zinc reagent. Iron element reagent 1-4 parts.

3. The hypoxia-resistant composition containing snow lotus culture according to claim 1, characterized in that, The hypoxia-resistant composition containing snow lotus culture comprises the following components in parts by weight: 175-200 portions of snow lotus culture, 17-22 portions of magnesium reagent, and 0.5-1 portion of zinc reagent. Iron element reagent 1.5-3 parts.

4. The hypoxia-resistant composition containing snow lotus culture according to claim 1, characterized in that, The magnesium element reagent is one or more of the following: magnesium citrate, magnesium gluconate, magnesium L-threonate, magnesium vitamin C phosphate, magnesium sulfate, magnesium chloride, magnesium oxide, magnesium carbonate, and magnesium hydrogen phosphate.

5. The hypoxia-resistant composition containing snow lotus culture according to claim 1, characterized in that, The zinc element reagent is one or more of the following: zinc sulfate, zinc gluconate, zinc glycinate, zinc oxide, zinc lactate, zinc citrate, zinc chloride, zinc acetate, zinc carbonate, zinc carnosine, zinc pyridinecarboxylate, and zinc-enriched yeast.

6. The hypoxia-resistant composition containing snow lotus culture according to claim 1, characterized in that, The iron element reagent is one or more of the following: ferrous sulfate, ferrous gluconate, ferric ammonium citrate, ferrous fumarate, ferric citrate, ferrous lactate, ferric heme chloride, ferric pyrophosphate, ferric porphyrin, ferrous glycinate, reduced iron, ferric sodium ethylenediaminetetraacetate, carbonyl iron powder, ferrous carbonate, ferrous citrate, ferrous fumarate, ferrous succinate, heme iron, and electrolytic iron.

7. The hypoxia-resistant composition containing snow lotus culture according to claim 1, characterized in that, The food-grade coating powder is one or more of hydroxypropyl methylcellulose, povidone, talc, and polyethylene glycol.

8. The method for preparing the hypoxia-resistant composition containing snow lotus culture as described in claim 1, characterized in that, The preparation process, which uses wet granulation and tableting, includes the following steps: (1) Pretreatment: The snow lotus culture was crushed to ≥100 mesh; (2) Mixing and granulation: First, mix the zinc reagent with an equal amount of isomaltitol to obtain mixed powder A; Mixed powder A with an equal amount of isomaltitol to obtain mixed powder B; Mixed powder B is mixed with iron element reagent to obtain mixed powder C; Mixed powder C with an equal amount of isomaltitol yielded mixed powder D; The pulverized snow lotus culture, hydroxypropyl methylcellulose, mixed powder D, magnesium element reagent, sucralose, and the remaining isomaltitol were added to the mixing granulator in sequence and stirred for 15-20 minutes to obtain the mixture. Then, sodium carboxymethyl cellulose was prepared into an aqueous solution with a mass concentration of 1%-2% as a binder, added to the mixture, and then granulated to obtain wet granules. (3) Drying: The wet granules are spread evenly in a fluidized bed dryer to dry, and granules are obtained; (4) Mixing: Add the granules and magnesium stearate to a mixer, mix well, compress into tablets, and coat to obtain the finished product.

9. The method for preparing a hypoxia-resistant composition containing snow lotus culture according to claim 8, characterized in that, The mixing granulator has a stirring paddle speed of 15 Hz to 25 Hz, a blade speed of 40 Hz to 50 Hz, and a mixing time of 15 to 20 minutes.

10. The method for preparing a hypoxia-resistant composition containing snow lotus culture according to claim 8, characterized in that, The dryer has a motor frequency of 30-40 Hz, an inlet air temperature of 50-70°C, an outlet air temperature of 45-50°C, and dries the particles to a moisture content of 2%-4%.

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