A mild multi-effect scrub containing biosugar lipids and amino acid surfactants and a preparation method thereof

Abstract

The application discloses a mild multi-effect scrub containing biosugar lipids and amino acid surfactants and a preparation method thereof. Sodium cocoyl glycinate, biosugar lipids, nicotinamide and hydrolyzed yeast extract are used as core components, physical scrub particles are matched, and cosmetic acceptable carriers such as polyols and thickening agents are compounded, and the mild multi-effect scrub is prepared through processes such as phase solubility, homogenization, temperature reduction and dispersion. The application reduces the irritation of the product from the two dimensions of chemical cleaning and physical friction, realizes the cleaning and exfoliation, simultaneously achieves the multiple effects of skin color lightening, bacteriostasis and acne removal, the core components have synergistic effects, the problems of strong irritation, single function and large environmental burden of traditional scrubs are solved, the product is suitable for sensitive skin, has environmental friendliness and verifiable efficacy, and has a wide application prospect.

Description

Technical Field

[0001] This invention belongs to the field of cosmetic technology, specifically relating to a mild, multi-effect scrub containing bio-glycolipids and amino acid surfactants, and its preparation method. Background Technology

[0002] Body scrubs, as a common physical exfoliating skincare product, work by using the physical friction generated when the abrasive particles in the product are massaged onto the skin's surface. This process removes dead skin cells from the stratum corneum, promotes skin metabolism, and improves skin roughness and dullness, thus achieving a skin-renewing effect. They are widely used in daily personal care.

[0003] As consumers' skincare concepts evolve, higher demands are being placed on the gentleness, functionality, and environmental friendliness of exfoliating scrubs. Traditional scrubs and existing technologies are gradually revealing numerous shortcomings. For example, patent application CN121287554A (publication date: January 9, 2026) attempts to introduce amino acid surfactants to replace traditional irritating surfactants to reduce skin irritation. However, this technology only utilizes the gentle properties of amino acid surfactants and lacks a synergistic design with bio-glycolipid surfactants. It fails to fully leverage the synergistic effects of both surfactants in gentle cleansing and barrier protection, and its product efficacy remains focused on basic exfoliation, resulting in a relatively limited functionality. Furthermore, most exfoliating scrubs on the market currently suffer from limited efficacy, only providing basic physical exfoliation and failing to meet consumers' diverse skincare needs, such as brightening, antibacterial and acne-reducing effects, and microecological regulation. In addition, some scrubs claiming functionalities lack clear and reliable efficacy data, making it difficult to scientifically demonstrate their effectiveness and thus hindering consumer trust.

[0004] Specifically, the technical defects of traditional scrubs and existing related technologies are mainly reflected in the following three aspects: (1) Prominent irritation problem: Traditional scrubs mostly use sulfate surfactants (such as SLS / SLES) as cleaning ingredients. These surfactants have excessive cleaning power. When working together with physical scrubbing particles, they are prone to excessively peeling the stratum corneum of the skin, damaging the integrity of the skin barrier, and causing the skin to lose water through the epidermis (TEWL) to increase. This is consistent with the typical characteristics of irritating products in the skin barrier function assessment, which in turn causes discomfort symptoms such as dryness, tightness, itching, and even sensitivity of the skin. It is especially unsuitable for use on fragile skin types such as sensitive skin. Moreover, the irritation of traditional surfactants has been confirmed by methods such as red blood cell hemolysis test, and their biocompatibility is poor. (2) Single function and insufficient efficacy demonstration: Most existing scrubs rely solely on physical exfoliating particles to achieve exfoliation. Simple physical exfoliation cannot simultaneously improve skin pigmentation or regulate the skin's surface microecological balance. Imbalance in the skin's surface microecology can easily lead to the accumulation of harmful microorganisms, causing problems such as clogged pores and inflammation. Traditional scrubs lack corresponding regulatory mechanisms. At the same time, although some functional scrubs claim to have brightening and antibacterial effects, they do not provide clear experimental data and mechanism explanations, making it difficult to demonstrate the scientific validity and effectiveness of their efficacy and failing to meet consumers' high-quality demand for multi-effect skincare. (3) Environmental burden: Some traditional scrubs use plastic microbeads (such as PE microbeads) as physical exfoliating particles. These plastic microbeads are difficult to degrade by the natural environment. After use, they are discharged into water bodies and soil, causing continuous pollution to the ecological environment, which is not in line with the current trend of green and environmentally friendly skincare development. Furthermore, the relevant technical solutions for the application of natural biodegradable exfoliating particles have not yet been effectively combined with a mild surfactant system.

[0005] In addition, bioactive glycolipids (such as rhamnolipids) are green and mild biosurfactants that can selectively remove oxidized lipids through intelligent cleansing mechanisms while protecting the skin barrier structure. They also have certain antibacterial and moisturizing effects. When combined with amino acid surfactants, they can achieve a synergistic enhancement of gentle cleansing and skin care effects. This synergistic effect has been initially applied in some cleansing products, but it has not yet been applied to scrub products to address the shortcomings of existing technologies.

[0006] In summary, existing scrub products suffer from technical defects such as strong irritation, limited functionality, insufficient efficacy verification, and significant environmental impact. The market urgently needs a scrub product that can achieve gentle cleansing, simultaneously brighten skin tone, inhibit bacteria and remove acne, and is environmentally friendly with verifiable efficacy.

[0007] Therefore, developing a mild, multi-effect scrub containing bio-glycolipids and amino acid surfactants, and its preparation method, to overcome the aforementioned shortcomings of existing technologies, has significant market value and application prospects. Summary of the Invention

[0008] To address the problems of existing technologies, this invention provides a gentle, multi-functional scrub containing bio-glycolipids and amino acid surfactants. Its formula achieves multiple benefits through a combination and specific ratio of key ingredients: sodium cocoyl glycinate, niacinamide, bio-glycolipids, and hydrolyzed yeast extract. Sodium cocoyl glycinate and bio-glycolipids create a low-irritation exfoliation mechanism—the former reduces skin friction through gentle foaming, while the latter provides both gentle cleansing and post-cleansing moisturizing, relieving dryness. Together, they achieve the effect of "exfoliating without damaging the skin barrier." Niacinamide brightens the complexion and reduces post-scrub pigmentation by inhibiting melanin transfer. Hydrolyzed yeast extract targets and inhibits Propionibacterium acnes and Staphylococcus aureus, effectively alleviating breakouts.

[0009] On the one hand, the present invention provides a mild multi-effect scrub containing bio-glycolipids and amino acid surfactants, which includes core functional ingredients and a cosmetically acceptable carrier. The core functional ingredients include sodium cocoyl glycinate, bio-glycolipid surfactants, niacinamide, hydrolyzed yeast extract and physical exfoliating particles.

[0010] Specifically, the bio-glycolipid surfactant is sophorolipid surfactant S-100C, which is composed of Candida albicans, glucose, methyl rapeseed oil fermentation product and water. Preferably, by weight percentage, the Candida albicans, glucose and methyl rapeseed oil fermentation product are 20%-35% and water is 65%-80%.

[0011] Specifically, the physical abrasive particles are selected from spherical hickory shell powder, preferably hickory shell powder with a diameter of 250-500μm.

[0012] Specifically, by weight percentage, the added amounts of each core ingredient are: sodium cocoyl glycinate 3.0%-40.0%, sophorolipid 0.5%-15%, niacinamide 0.5%-5.0%, physical exfoliating particles 0.1%-15.0%, and hydrolyzed yeast extract 0.1%-20%. Preferably, the added amounts of each core ingredient are: sodium cocoyl glycinate 5.0%-20.0%, sophorolipid 3%-10%, niacinamide 1.0%-3.0%, physical exfoliating particles 1.0%-8.0%, and hydrolyzed yeast extract 1.0%-5.0%.

[0013] Specifically, the acceptable carriers for the cosmetic include one or more of the following: water, polyol moisturizers, thickeners, binders, pH adjusters, emollients, preservatives, and surfactant complexes.

[0014] Specifically, the polyol moisturizer is glycerin and / or butylene glycol; the thickener is hydroxyethyl cellulose, xanthan gum and / or acrylate copolymer; the emollient is squalane and / or jojoba oil; the binder is lacquer fruit wax; the viscosity control agent is sodium chloride; and the preservative is HanacoPurexiderm RX-3.

[0015] Specifically, it also contains cocamidopropyl betaine, sodium lauroyl glutamate, and sea salt.

[0016] Specifically, the amounts of each component added, by weight percentage, are as follows: deionized water 2.0%-8.0%, glycerin 1.0%-10%, hydroxyethyl cellulose 0.01%-1.0%, lacquer wax 0.1%-5.0%, cocamidopropyl betaine 0.1%-10.0%, sodium lauroyl glutamate 0.1%-10.0%, squalane 0.1%-5.0%, jojoba oil 0.1%-4.0%, acrylate copolymer 1.0%-20.0%, sodium chloride 30.0%-70.0%, sea salt 0.5%-5.0%, and preservatives 0.1%-20%.

[0017] Preferably, the amounts of each component added, by weight percentage, are as follows: deionized water 2.0%-6.3%, glycerin 3.0%-6%, hydroxyethyl cellulose 0.1%-0.5%, lacquer wax 0.1%-3.0%, cocamidopropyl betaine 1.0%-6.0%, sodium lauroyl glutamate 1.0%-5.0%, squalane 2.0%-4.0%, jojoba oil 1.0%-3.0%, acrylate copolymer 3.0%-10.0%, sodium chloride 40.0%-60.0%, sea salt 1.5%-4.0%, and preservative 1.0%-5.0%.

[0018] On the other hand, the present invention also provides a method for preparing the aforementioned scrub, comprising the following steps: (1) heating and stirring phase A, composed of water, glycerin and hydroxyethyl cellulose, to 60-70°C until completely dissolved; (2) keeping the temperature at 60-70°C, adding phase B and dispersing evenly; (3) adding phase C, composed of squalane and jojoba oil, and dispersing evenly; (4) homogenizing phases A, B and C for 3-5 min at a homogenization speed of 3000-5000 r / min; (5) cooling and keeping the temperature at 45-50°C, adding phase D, composed of acrylate copolymer and water, while stirring, and dispersing evenly; (6) adding phase E, stirring at low speed, and dispersing evenly to obtain the product.

[0019] Specifically, phase A consists of deionized water, glycerol, and hydroxyethyl cellulose; phase B consists of urushiol wax, sodium cocoyl glycinate, cocamidopropyl betaine, sodium lauroyl glutamate, and sophorolipid; phase E consists of sodium chloride, sea salt, nicotinamide, physical abrasive particles, and preservatives; preferably, the addition amounts of each component, by weight percentage, are: deionized water 2.0%-8.0%, glycerol 1.0%-10%, and hydroxyethyl cellulose 0.01%-1.0%. %, 0.1%-5.0% of lacquer fruit wax, 3.0%-40.0% of sodium cocoyl glycinate, 0.1%-10.0% of cocamidopropyl betaine, 0.1%-10.0% of sodium lauroyl glutamate, 0.5%-15% of sophorolipid, 0.1%-5.0% of squalane, 0.1%-4.0% of jojoba oil, 1.0%-20.0% of acrylate copolymer, 30.0%-70.0% of sodium chloride, 0.5%-5.0% of sea salt, and 0.0% of nicotinamide. 5%-5.0%, physical exfoliating particles 0.1%-15.0%, preservatives 0.1-20%, hydrolyzed yeast extract 0.1-20%; more preferably, by weight percentage, the amounts of each component are: deionized water 2.0%-6.3%, glycerin 3.0%-6%, hydroxyethyl cellulose 0.1%-0.5%, wild lacquer wax 0.1-3.0%, sodium cocoyl glycinate 5.0%-20.0%, cocamidopropyl betaine 1.0%-6%. 0.0%, Sodium lauroyl glutamate 1.0%-5.0%, Sophorolipin 3%-10%, Squalane 2.0%-4.0%, Jojoba oil 1.0%-3.0%, Acrylate copolymer 3.0%-10.0%, Sodium chloride 40.0%-60.0%, Sea salt 1.5%-4.0%, Nicotinamide 1.0%-3.0%, Physical exfoliating particles 1.0%-8.0%, Preservatives 1.0%-5.0%, Hydrolyzed yeast extract 1.0%-5%.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. Achieves extreme low irritation and high tolerance during the cleansing and exfoliation process: Traditional scrubs often use strong anionic surfactants (such as SLS) with sharp, coarse particles, which can easily damage the skin barrier when removing dead skin cells. This invention uses sophorolipid surfactant and sodium cocoyl ester as core cleansing ingredients (whose pH is similar to that of the skin, making them significantly gentler than traditional surfactants), and limits the use of specific spherical, smooth physical abrasive particles (walnut shell powder). By synergistically reducing irritation from both the chemical cleansing system and the physical friction system, the product is especially suitable for sensitive skin.

[0022] 2. Beyond simple exfoliation, this product achieves a holistic approach to "cleansing, brightening, and acne treatment": Existing scrub products focus on physical cleansing, lacking immediate and long-term management of the skin's condition after exfoliation. This invention creatively introduces two active ingredients with specific functions into the formula: niacinamide and hydrolyzed yeast extract. Niacinamide, as a skin-brightening ingredient, can improve dull skin with long-term use. Hydrolyzed yeast extract, as a new generation of antibacterial protein, gently cleanses while acting on the skin surface during use, effectively targeting and inhibiting Propionibacterium acnes and Staphylococcus aureus, effectively reducing acne formation. The three components form a comprehensive system of cleansing, brightening, and acne treatment, providing multifunctional, all-around care.

[0023] 3. Synergistic Effect Between Core Ingredients: This invention is not a simple accumulation of ingredients, but rather a specific combination and ratio that allows the gentle cleansing of sodium cocoyl glycinate and sophorolipid surfactants to create optimal conditions for the action of subsequent active ingredients. At the same time, the effective antibacterial properties of hydrolyzed yeast extract and the immediate brightening effect of niacinamide work together to achieve comprehensive efficacy in maintaining the healthy appearance of the skin while also ensuring functionality. This solves the technical problem of the disconnect between "cleansing" and "skincare" in traditional products. Furthermore, the exfoliating particles are biodegradable, in line with the trend of green environmental protection. The product efficacy is supported by clear experimental data, and its scientific validity and effectiveness can be verified. Attached Figure Description

[0024] Figure 1 This is a schematic diagram showing the effect of sophoroliposide exogenous activity S-100C on the relative expression level of the aqp3a gene.

[0025] Figure 2 This is a diagram illustrating the melanin-clearing effects of different concentrations of niacinamide.

[0026] Figure 3 These are images demonstrating the cleaning effect of the scrub, with A, C representing the effects before testing, after application, and after cleaning, respectively.

[0027] Figure 4 This is a demonstration of the results of the HET-CAM chicken embryo chorioallantoic membrane test to verify the irritant effect of the scrub. A is the chicken embryo chorioallantoic membrane before scrub treatment, and B is the chicken embryo chorioallantoic membrane after 5 minutes of scrub treatment. Detailed Implementation

[0028] The present invention will be further described below with reference to specific embodiments, and the advantages and features of the present invention will become clearer as a result of the description. However, these embodiments are merely illustrative and do not constitute any limitation on the scope of protection defined by the claims of the present invention.

[0029] It should be understood that the terminology used in this invention is merely for describing particular embodiments and is not intended to limit the invention. Furthermore, with respect to numerical ranges in this invention, it should be understood that the upper and lower limits of the range and each intermediate value between them are specifically disclosed. Any stated value or intermediate value within a stated range, as well as each smaller range between any other stated value or intermediate value within said range, are also included in this invention. The upper and lower limits of these smaller ranges may be independently included or excluded from the range.

[0030] Unless otherwise stated, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains. While only preferred methods and materials have been described herein, any methods and materials similar or equivalent to those described herein may be used in the implementation or testing of this invention. All references to this specification are incorporated by way of citation to disclose and describe methods and / or materials associated with those references. In the event of any conflict with any incorporated reference, the content of this specification shall prevail.

[0031] Example 1: Selection of Components in the Formula of Exfoliating Scrub

[0032] 1. Component selection

[0033] (1) Sodium cocoyl glycinate: As an anionic amino acid surfactant, it provides gentle cleansing and delicate foam. Studies have shown through data such as corn protein solubility determination and use tests on patients with skin diseases such as atopic dermatitis that sodium cocoyl glycinate is ultra-mild and causes minimal damage to the lipid and protein layers of the stratum corneum. In actual use, it shows good cleansing performance and skin feel (Navare B, Thakur S, Nakhe S. A review on Surfactants: Role in skin irritation, SC damage, and effect of mild cleansing over damaged skin[J]. International Journal of Advance Research, Ideas and Innovations in Technology, 2019, 5:1077-1081).

[0034] (2) Sophorolipid (a type of biological glycolipid emulsifier, commercially known as S-100C): Its composition consists of Candida albicans, glucose, methyl rapeseed oil fermentation product and water.

[0035] Experiments were conducted to verify its skin irritation and moisturizing properties, as detailed below:

[0036] ① Irritation test of S-100C: Sophorolipid S-100C was used. Approximately 24 hours before the experiment, the hair on both sides of the spine on the back of the test animals was shaved without damaging the epidermis. The shaved area was approximately 3cm × 3cm on both the left and right sides. 0.5mL of sophorolipid was applied to the shaved skin on the left side with an area of ​​2.5cm × 2.5cm, with the other side serving as a control. The application was performed once a day for 14 consecutive days. Starting from the second day, the hair was shaved before each application, and any residual test substance was removed with warm water. The local skin reaction was observed and scored 1 hour later. The results are shown in Table 1. In multiple skin irritation tests on New Zealand rabbits, the average score per animal per day was 0. According to the skin irritation intensity classification of the "Cosmetic Safety Technical Specifications" (2015 edition), it is considered non-irritating.

[0037] Table 1. Results of Stimulus Response Test

[0038]

[0039] ②S-100C Moisturizing Test:

[0040] Experimental principle:

[0041] Zebrafish treated with sodium chloride will experience water loss and wrinkling of the skin surface due to osmotic pressure. Hyaluronic acid (HA) is present in the dermis and has moisturizing properties. Aquaporins (AQPs) are proteins located on cell membranes, forming "channels" that control the movement of water in and out of cells. AQP-3, or aquaporin 3, is primarily an epidermal aquaporin, a membrane transport protein for water and glycerol. It is expressed in the plasma membrane of keratinocytes in the basal layer of the epidermis of normal skin, promoting water permeability and stratum corneum hydration. Therefore, detecting the relative expression level of the aqp3a gene can indicate whether a sample has moisturizing effects.

[0042] Methods: i. Zebrafish were randomly selected and placed in 6-well plates, with 30 fish per well; ii. The samples were administered in water, and a normal control group and a model control group were set up. The normal control group consisted of zebrafish that did not receive any treatment during the experiment, which reflected the skin hydration status of zebrafish under natural conditions; the model control group consisted of zebrafish treated with sodium chloride aqueous solution, whose surface would shrink due to osmotic pressure difference, serving as a positive control for evaluating the efficacy of the test substance; the S100C experimental group consisted of zebrafish placed in a culture plate containing 0.0012% sophorolipid S-100C + sodium chloride aqueous solution, and the culture plate was incubated in a constant temperature incubator at 28℃ for 20 min (simulating the dehydration process under hypertonic conditions). The zebrafish were then removed, and the volume of each well was 3 mL, with three biological replicates; iii. A zebrafish skin dehydration model was established by simultaneously administering sodium chloride in water, and the zebrafish were incubated at 28℃ in the dark for 22 h; iv. Total RNA was extracted from zebrafish in each experimental group, cDNA was synthesized, and the gene expression of β-actin and the target gene was detected by q-PCR. β-actin was used as an internal control for gene expression, and the relative RNA expression level of the target gene was calculated. The results are shown in Table 2. Figure 1 As shown, the relative expression level of the aqp3a gene in the sophorolipid surfactant S-100C was significantly increased compared with that in the model control group, revealing that the sample has moisturizing effects.

[0043] Table 2. Detection of relative expression levels of the aqp3a gene

[0044]

[0045] (3) Niacinamide (Vitamin B3): Niacinamide can inhibit melanin transfer, thereby improving uneven skin tone. Experimental principle: The skin brightening effect of niacinamide is evaluated by detecting the melanin content in cells.

[0046] Methods: i) Sample solutions of different concentration gradients were used (experimental group: 0.25mM nicotinamide, 3mM nicotinamide, 10mM nicotinamide, positive control group: 1mM kojic acid), and the samples were collected after 24 hours of treatment.

[0047] ii) Centrifuge again at 1000 rpm for 5 min, discard the supernatant, add 1 mL PBS to wash the cells, gently pipette the suspension to resuspend the cells, and repeat this step twice.

[0048] iii) Add 650 μL of 1M NaOH solution containing 10% DMSO, gently pipette to resuspend the cells, and place in a water bath at 80°C for 2 hours.

[0049] iv) After water bath, transfer 200 μL to each well into a clean 96-well plate. Perform 3 replicates per group and measure the absorbance at 405 nm using a microplate reader.

[0050] v) Calculate the melanin content inhibition rate of B16-F10 cells based on the OD values ​​of each experimental group. The formula is: Melanin content inhibition rate (%) = (1 - (OD value of experimental group / OD value of control group)) 100%.

[0051] The effect of the sample on melanin was evaluated based on the calculated inhibition rate, and the skin brightening effect of the sample was assessed.

[0052] The results are shown in Table 3 and Figure 2 As shown, niacinamide at concentrations of 0.25 and 3 mM exhibited melanin-clearing effects on B16-F10 cells, with a melanin clearance rate of 37.66% at 0.25 mM. The positive control, 1 mM kojic acid, showed a melanin clearance rate of 11.69%. Melanin clearance effect: niacinamide > kojic acid. Based on the above data, niacinamide can reduce melanin production and has a certain skin-brightening effect.

[0053] Table 3. Effects of different concentrations of nicotinamide on melanin

[0054]

[0055] (4) Hydrolyzed yeast extract: The material composition is yeast fermentation product extract and water. The quantitative antibacterial test of suspension was carried out using WS / T650-2019 "Evaluation Method for Antibacterial and Antimicrobial Effects" 5.1.1, with a 1000-fold dilution. The results are shown in Table 4. The antibacterial rate of hydrolyzed yeast extract is ≥90%. According to WS / T650-2019 "Evaluation Method for Antibacterial and Antimicrobial Effects", the antibacterial rate is >50-90%, and the product has antibacterial effect. Therefore, the antibacterial protein developed by hydrolyzed yeast extract has a strong inhibitory effect on Propionibacterium acnes.

[0056] Table 4. Results of antibacterial rate test of hydrolyzed yeast extract

[0057]

[0058] (5) Physical abrasive particles: mainly commercially available (Dinghui Industrial (Guangzhou) Co., Ltd.) spherical pecan shell powder (250-500μm), which avoids scratching the skin and is biodegradable.

[0059] 2. Proportioning

[0060] The above components were combined with cosmetically acceptable carriers, including water, polyol moisturizers (glycerin, butylene glycol), thickeners (xanthan gum, hydroxyethyl cellulose), and pH adjusters (citric acid / sodium citrate) to prepare a scrub. The formulation of each component is shown in Table 5.

[0061] Table 5. Proportions of each component in the scrub

[0062]

[0063] Manufacturing process:

[0064] (1) Phase A: Heat and stir to approximately 65-70℃ until completely dissolved;

[0065] (2) Keep warm at 65-70℃, add phase B, and disperse evenly;

[0066] (3) Add phase C and disperse evenly;

[0067] (4) Homogenize ABC, 3-5 min, 3000-5000 r / min;

[0068] (5) Keep warm to 45-50℃, add phase D while stirring, and disperse evenly;

[0069] (6) Add phase E, stir at low speed until evenly dispersed.

[0070] Example 2: Scrub Effect Test

[0071] 1. Cleaning effect test

[0072] (1) Stain preparation:

[0073] Wisence Black-AS BL-100P-SM: 38.8wt%

[0074] Squalane: 35wt%

[0075] Polydimethylsiloxane 100cs: 25wt%

[0076] Preservative RX-3: 1.2wt%

[0077] (2) Dirt dosage: 3 mg / cm 2

[0078] (3) Test site: inner side of both arms (forearms), symmetrical area.

[0079] (4) Sample volume to be tested: 2 mg / cm³ 2

[0080] (5) Test method: Apply the dirt evenly to the inside of the arm, take the sample to be tested, apply it to the dirt, massage gently for 30 seconds with a fixed technique, rinse with pure water at a flow rate of 3 L / min (or at a fixed flow rate) for 20 seconds, pat dry, and let stand for 10 minutes.

[0081] (6) Cleaning effect: The results are as follows Figure 3As shown, A and C represent the effects before testing, after application, and after cleaning, respectively. This cleaning effect demonstrates that the complex composed of sodium cocoyl glycinate, bioactive glycolipid surfactant S-100C, niacinamide, and hydrolyzed yeast extract has excellent cleaning properties.

[0082] 2. Stimulation verification

[0083] The irritant properties of the scrub were verified using the HET-CAM chicken embryo chorioallantoic membrane test.

[0084] Method: 1 wt% of the exfoliating scrub prepared according to the formula in Example 1 was mixed with water and used to treat chicken embryos (chicken embryo incubation conditions: incubator temperature: 37.8℃; relative humidity: 60%). Using a micropipette, 0.3 mL of the liquid test substance was evenly applied to the center of the CAM (chorioallantoic membrane of chicken embryo) (covering approximately 50% of the area). After contact for 3 minutes, the CAM surface was gently rinsed with 0.9% sodium chloride solution (completed within 30 seconds) to remove residual test substance. The vascular reaction (bleeding, coagulation, vascular dissolution) of the CAM was observed at 0 and 5 minutes after rinsing. The mildness of the test substance was assessed based on its irritant state.

[0085] Scoring criteria: Stimulation score is IS. IS < 1, no irritation; 1 < IS < 5, mild irritation; 5 ≤ IS < 9, moderate irritation; IS ≥ 10, strong irritation / corrosiveness.

[0086] The results are as follows Figure 4 As shown, A is the chicken embryo chorioallantoic membrane before treatment with the scrub, and B is the chicken embryo chorioallantoic membrane after 5 minutes of treatment with the scrub, proving that the scrub developed in this invention is non-irritating.

[0087] The embodiments described above are merely preferred embodiments of the present invention and are not intended to limit the scope of the present invention. Various modifications and improvements made by those skilled in the art to the technical solutions of the present invention without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.

Claims

1. A mild, multi-effect scrub containing bio-glycolipids and amino acid surfactants, characterized in that, It includes core functional ingredients and cosmetically acceptable carriers, wherein the core functional ingredients include sodium cocoyl glycinate, bioactive glycolipid surfactants, niacinamide, hydrolyzed yeast extract, and physical exfoliating particles.

2. The exfoliating scrub according to claim 1, characterized in that, The bio-glycolipid surfactant is sophorolipid surfactant S-100C, which is composed of Candida albicans, glucose, methyl rapeseed oil fermentation product and water. Preferably, by weight percentage, the Candida albicans, glucose and methyl rapeseed oil fermentation product are 20%-35% and water is 65%-80%.

3. The exfoliating scrub according to claim 1, characterized in that, The physical abrasive particles are selected from spherical pecan shell powder, preferably pecan shell powder with a diameter of 250-500μm.

4. The exfoliating scrub according to claim 1, characterized in that, The added amounts of each core ingredient, by weight percentage, are: sodium cocoyl glycinate 3.0%-40.0%, sophorolipid 0.5%-15%, niacinamide 0.5%-5.0%, physical exfoliating particles 0.1%-15.0%, and hydrolyzed yeast extract 0.1%-20%. Preferably, the added amounts of each core ingredient are: sodium cocoyl glycinate 5.0%-20.0%, sophorolipid 3%-10%, niacinamide 1.0%-3.0%, physical exfoliating particles 1.0%-8.0%, and hydrolyzed yeast extract 1.0%-5.0%.

5. The exfoliating scrub according to claim 1, characterized in that, The acceptable carriers for the cosmetic include one or more of the following: water, polyol moisturizers, thickeners, binders, pH adjusters, emollients, preservatives, and surfactants.

6. The exfoliating scrub according to claim 5, characterized in that, The polyol moisturizer is glycerin and / or butylene glycol; the thickener is hydroxyethyl cellulose, xanthan gum and / or acrylate copolymer; the emollient is squalane and / or jojoba oil; the binder is lacquer fruit wax; the viscosity control agent is sodium chloride; and the preservative is HanacoPurexiderm RX-3.

7. The exfoliating paste according to claim 6, characterized in that, It also contains cocamidopropyl betaine, sodium lauroyl glutamate, and sea salt.

8. The exfoliating scrub according to claim 8, characterized in that, The following components, by weight percentage, are added as follows: deionized water 2.0%-8.0%, glycerin 1.0%-10%, hydroxyethyl cellulose 0.01%-1.0%, lacquer wax 0.1%-5.0%, cocamidopropyl betaine 0.1%-10.0%, sodium lauroyl glutamate 0.1%-10.0%, squalane 0.1%-5.0%, jojoba oil 0.1%-4.0%, acrylate copolymer 1.0%-20.0%, sodium chloride 30.0%-70.0%, sea salt 0.5%-5.0%, and preservatives 0.1%-20%. Preferably, the amounts of each component added, by weight percentage, are as follows: deionized water 2.0%-6.3%, glycerin 3.0%-6%, hydroxyethyl cellulose 0.1%-0.5%, lacquer wax 0.1%-3.0%, cocamidopropyl betaine 1.0%-6.0%, sodium lauroyl glutamate 1.0%-5.0%, squalane 2.0%-4.0%, jojoba oil 1.0%-3.0%, acrylate copolymer 3.0%-10.0%, sodium chloride 40.0%-60.0%, sea salt 1.5%-4.0%, and preservative 1.0%-5.0%.

9. A method for preparing a scrub as described in any one of claims 1-8, characterized in that, The process includes the following steps: (1) Heat and stir the A phase, which consists of water, glycerol, and hydroxyethyl cellulose, to 60-70°C until completely dissolved; (2) Keep the temperature at 60-70°C, add the B phase and disperse evenly; (3) Add the C phase, which consists of squalane and jojoba oil, and disperse evenly; (4) Homogenize the A, B, and C phases for 3-5 minutes at a speed of 3000-5000 r / min; (5) Cool down and keep the temperature at 45-50°C, while stirring, add the D phase, which consists of acrylate copolymer and water, and disperse evenly; (6) Add the E phase, stir at low speed, and disperse evenly to obtain the final product.

10. The preparation method according to claim 9, characterized in that, Phase A consists of deionized water, glycerin, and hydroxyethyl cellulose; Phase B consists of urushiol wax, sodium cocoyl glycinate, cocamidopropyl betaine, sodium lauroyl glutamate, and sophorolipid; Phase E consists of sodium chloride, sea salt, nicotinamide, physical abrasive particles, and preservatives; preferably, the amounts of each component added, by weight percentage, are: deionized water 2.0%-8.0%, glycerin 1.0%-10%, hydroxyethyl cellulose 0.01%-1.0%, urushiol wax, sodium cocoyl glycinate, sodium cocoyl glutamate, and sophorolipids; Phase E consists of sodium chloride, sea salt, nicotinamide, physical abrasive particles, and preservatives. The ingredients are: lacquer wax 0.1-5.0%, sodium cocoyl glycinate 3.0%-40.0%, cocamidopropyl betaine 0.1%-10.0%, sodium lauroyl glutamate 0.1%-10.0%, sophorolipid 0.5%-15%, squalane 0.1%-5.0%, jojoba oil 0.1%-4.0%, acrylate copolymer 1.0%-20.0%, sodium chloride 30.0%-70.0%, sea salt 0.5%-5.0%, and nicotinamide 0.5%. -5.0%, physical abrasive particles 0.1%-15.0%, preservatives 0.1%-20%, hydrolyzed yeast extract 0.1%-20%; more preferably, the amounts of each component added, by weight percentage, are: deionized water 2.0%-6.3%, glycerin 3.0%-6%, hydroxyethyl cellulose 0.1%-0.5%, lacquer fruit wax 0.1%-3.0%, sodium cocoyl glycinate 5.0%-20.0%, and cocamidopropyl betaine 1.0%-6%. 0%, Sodium lauroyl glutamate 1.0%-5.0%, Sophorolipin 3%-10%, Squalane 2.0%-4.0%, Jojoba oil 1.0%-3.0%, Acrylate copolymer 3.0%-10.0%, Sodium chloride 40.0%-60.0%, Sea salt 1.5%-4.0%, Nicotinamide 1.0%-3.0%, Physical exfoliating particles 1.0%-8.0%, Preservatives 1.0%-5.0%, Hydrolyzed yeast extract 1.0%-5%.

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