Method for simultaneously detecting retinoids and liposoluble vitamin metabolic marker groups in biological samples and matching reagents

By combining PLS magnetic bead purification and RP-amide chromatographic separation with positive and negative ion switching mass spectrometry detection, the sensitivity and applicability issues of retinol and fat-soluble vitamin metabolic marker detection in existing technologies have been solved, enabling efficient and simultaneous analysis of multiple biological samples.

CN122109383APending Publication Date: 2026-05-29BEIJING TSINGHUA CHANGGUNG HOSPITAL
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BEIJING TSINGHUA CHANGGUNG HOSPITAL
Filing Date
2026-03-31
Publication Date
2026-05-29

AI Technical Summary

Technical Problem

Existing detection technologies are difficult to achieve high-sensitivity and specific simultaneous detection of multiple retinol and fat-soluble vitamin metabolic markers, especially in distinguishing structurally similar isomers, and are not applicable to a variety of biological sample types.

Method used

A cross-species, cross-matrix full-spectrum analysis method was established by combining PLS magnetic bead purification technology with RP-amide chromatographic separation and positive and negative ion switching mass spectrometry detection. This method includes sample pretreatment, magnetic solid-phase extraction, and liquid chromatography-mass spectrometry detection, enabling the simultaneous separation and detection of retinoic acid, retinal, and fat-soluble vitamins.

Benefits of technology

It achieves efficient and accurate detection of retinoic acid isomers and fat-soluble vitamins, reaching pg/mL level sensitivity, applicable to various biological sample types, breaking through the baseline separation of 9-cis-RA/13-cis-RA, and providing a one-stop analysis platform across species and sample types.

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Abstract

The application discloses a method for simultaneously detecting retinoids and lipid-soluble vitamin metabolic marker groups in biological samples and matching reagents. The application is based on magnetic bead solid-phase extraction technology, significantly improves sample purification effect, and reduces matrix interference; in combination with a high-aqueous-phase reversed-phase chromatography system and a positive-negative ion switching multi-reaction monitoring mode, simultaneous separation and detection of seven retinoid molecules and a full spectrum of lipid-soluble vitamins are realized. The method has high sensitivity, strong specificity and wide detection range, and for the first time realizes one-stop analysis across species and sample types, thereby providing reliable platform support for clinical and scientific research applications.
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Citation Information

Patent Citations

  • CN116858963A