Propolis shampoo and method for making the same

Abstract

This invention discloses a propolis shampoo and its manufacturing method, belonging to the field of daily chemical technology. The shampoo contains pure water, propolis, PEG-40 hydrogenated castor oil, and components such as anionic surfactants, amphoteric surfactants, anti-dandruff agents, and moisturizers. The combination of propolis and PEG-40 hydrogenated castor oil solves the stability problem, and the multiple components synergistically enhance the oil control and dandruff removal effects. The manufacturing method employs key operations such as staged temperature control, step-by-step addition of premixes, and vacuum degassing, and precisely adjusts the pH to 4.5-5.5 to suit the scalp environment. This results in a propolis shampoo with an antibacterial rate of over 99.9% against Malassezia furfur and a significant inhibition of 5α-reductase activity. It also has excellent dandruff removal and oil control effects, is gentle and non-irritating, and has a stable system, making it suitable for various scalp and hair types and ideal for daily washing and care.

Description

Technical Field

[0001] This invention belongs to the field of daily chemical technology, specifically relating to a propolis shampoo and its preparation method. Background Technology

[0002] Propolis, as a natural active substance, contains small-molecule active ingredients (molecular weight less than 500 Daltons) such as phenolic acids, flavonoid monomers, terpenes, aldehydes, and ketones, possessing biological properties such as antibacterial, antioxidant, and anti-inflammatory effects. Propolis shampoo is a hair care product made with propolis as its core active ingredient, combined with surfactants, conditioning agents, and nourishing ingredients. Propolis shampoo is expected to achieve basic cleansing functions while improving scalp health through the active properties of propolis, such as relieving scalp inflammation and inhibiting the growth of harmful bacteria, thus meeting consumers' demand for functional shampoo products.

[0003] However, propolis shampoos currently on the market still have many problems: Poor ingredient stability: Propolis is rich in unsaturated substances, which are easily oxidized by environmental factors, directly causing discoloration and changes in texture, while also weakening its original efficacy. Furthermore, the core active ingredients in propolis, such as flavonoids, are sensitive to pH and temperature changes. Under different acidic or alkaline environments or temperature fluctuations, they are prone to decomposition and deterioration, not only reducing the stability of the shampoo but also causing a decrease in the content of its effective ingredients, thus affecting the product's actual efficacy.

[0004] Limited Efficacy: Commercially available propolis shampoos generally have low propolis concentrations. Furthermore, since the primary function of shampoo is cleansing, its contact time with the scalp and hair is relatively short, making it difficult for propolis to fully reach the scalp and exert its core antibacterial and anti-inflammatory effects. Simultaneously, significant differences exist in individual scalp conditions (such as sebum secretion and sensitivity) and hair types (such as dry, oily, and normal), leading to noticeable individual variations in the effects of propolis shampoos, making it impossible to guarantee a stable and significant improvement for most users. More specifically, in the two key areas of oil control and dandruff removal, commercially available products are clearly inadequate: Regarding oil control, their inhibitory effect on 5α-reductase, which regulates sebaceous gland function, is weak, making it difficult to effectively reduce scalp sebum secretion; regarding dandruff removal, their antibacterial effect against Malassezia furfur, which causes dandruff, is limited, failing to achieve the ideal antibacterial rate, thus failing to address the root cause of dandruff.

[0005] Formula optimization is challenging: To achieve a synergistic effect between cleansing, nourishing, and propolis efficacy, propolis needs to be rationally combined with other ingredients such as surfactants and conditioners. However, current technology struggles to find a formulation that balances the compatibility of all components. Simply increasing the propolis content to enhance efficacy can lead to difficulties in mixing with aqueous components, resulting in issues like layering and sedimentation. Conversely, reducing the propolis content or adjusting the proportions of other ingredients can sacrifice cleansing or nourishing effects, failing to balance the relationship between cleansing, nourishing, and propolis efficacy, thus limiting the overall performance of the product. Summary of the Invention

[0006] The purpose of this invention is to address the problems of poor stability of propolis components, limited efficacy, and difficulty in formula optimization in existing propolis shampoos. This invention provides a propolis shampoo and its manufacturing method, which improves the stability of the propolis shampoo, enhances its antibacterial, anti-inflammatory, oil-controlling, and dandruff-removing effects, and expands its application range to meet the cleaning and nourishing needs of different users.

[0007] To solve the above-mentioned technical problems, the present invention provides a propolis shampoo, comprising the following components by weight percentage: 64-68% pure water, 0.05-0.2% disodium EDTA, 0.1-0.3% polyquaternium-10, 15-18% anionic surfactant, 1-3% cetearyl alcohol, 1-2% stearic acid, 1-4% honey, 0.3-0.7% propolis, 2-3% PEG-40 hydrogenated castor oil, 1-2% moisturizer, 0.2-0.5% anti-dandruff agent, 1-2% thickener, 1-2% amphoteric surfactant, 3-4% pearlescent agent, 0.05-0.2% KF-982, 0.1-0.3% citric acid, and 0.3-0.5% fragrance.

[0008] This invention's propolis shampoo, through a scientific blend of propolis and PEG-40 hydrogenated castor oil, overcomes the solubility challenge of propolis in aqueous systems, effectively inhibiting its oxidative deterioration and significantly improving the stability of propolis's active ingredients, ensuring long-lasting efficacy. The small-molecule active ingredients in propolis (such as phenolic acids and flavonoids) synergistically work with anti-dandruff agents to enhance the inhibition of Malassezia furfur and the blocking of 5α-reductase, greatly enhancing dandruff removal and oil control effects, solving the problem of limited efficacy in traditional products. A reasonable ratio of anionic and amphoteric surfactants, combined with conditioning ingredients such as polyquaternium-10, moisturizers, and thickeners, ensures both cleansing power and nourishing effects, improving smoothness and rinsing experience. Disodium EDTA chelates metal ions, and citric acid adjusts the pH to suit the scalp environment, further ensuring product stability and gentleness, suitable for various scalp and hair types, effectively solving the problems of difficult optimization and narrow applicability of traditional formulas.

[0009] As a further description of the above technical solution: the anionic surfactants include AESA, K12A, and AES. AESA is mild and low in irritation, produces fine foam, and has excellent stability; K12A has strong detergency and can effectively remove scalp oil and dirt; AES has excellent solubility and can function stably in a wide temperature range and under different pH conditions, with a balanced foaming and detergency performance. The combination of the three ensures sufficient cleaning power to meet basic shampooing needs, while avoiding excessive degreasing that can lead to scalp dryness or sensitivity. It also has good compatibility with ingredients such as propolis and PEG-40 hydrogenated castor oil in the formula, and will not interfere with the stability of the active ingredients of propolis or the effectiveness of its dandruff-removing and oil-controlling effects. At the same time, its excellent foaming performance can improve the richness and feel of the foam during use, and the combination with amphoteric surfactants further optimizes its mildness, making the product suitable for various scalp and hair types.

[0010] As a further description of the above technical solution: the amphoteric surfactant is CAB-35 (cocamidopropyl betaine), which combines the characteristics of both anionic and cationic surfactants. It is extremely mild and can effectively reduce the irritation that anionic surfactants may cause, making it suitable for sensitive scalps and solving the problem of balancing cleansing power and mildness in traditional shampoos and conditioners. At the same time, CAB-35 has excellent compatibility with ingredients such as propolis, PEG-40 hydrogenated castor oil, and anti-dandruff agents. It can enhance the overall stability of the formula through synergistic effects, avoid problems such as layering and sedimentation, and optimize formula compatibility. In addition, CAB-35 has good foaming properties and can work synergistically with anionic surfactants to produce rich and delicate foam, improving the feel and cleansing experience during use. It also has a certain conditioning effect, which can reduce hair friction. Combined with ingredients such as polyquaternium-10, it can enhance hair smoothness and further improve the overall value of the product.

[0011] As a further description of the above technical solution: the moisturizer is at least one of glycerin, butylene glycol, oat β-glucan, or sodium PCA. Glycerin, as a classic small-molecule moisturizer, can quickly replenish and lock in moisture. It has excellent compatibility with ingredients such as PEG-40 hydrogenated castor oil and surfactants in the formula, and is non-sticky, avoiding aggravating the burden on oily scalps. Butylene glycol can promote the penetration and absorption of small-molecule active ingredients (such as phenolic acids and flavonoids) in propolis while moisturizing, enhancing the synergistic effect of antibacterial and anti-inflammatory effects. Oat β-glucan has both long-lasting moisturizing and anti-inflammatory repair effects, which can relieve scalp inflammation caused by dryness or bacterial stimulation. It complements the anti-inflammatory effect of propolis and helps reduce dandruff. Sodium PCA, as a natural moisturizing factor of the skin, can simulate the scalp's own moisturizing mechanism, gently regulate the moisture content of the stratum corneum, avoid the imbalance of "dry outside and oily inside", and help control oil from the root. These ingredients are flexibly combined to ensure the scalp's basic moisturizing needs without interfering with the stability of propolis or the activity of anti-dandruff and oil-controlling ingredients. They are also suitable for various skin types, including sensitive scalps, further enhancing the product's gentleness and synergistic efficacy.

[0012] As a further description of the above technical solution: the dandruff-reducing agent is at least one of selenium disulfide, salicylic acid, or zinc pyrithione (ZPT). Selenium disulfide has outstanding broad-spectrum antibacterial properties, effectively inhibiting Malassezia furfur, which causes dandruff, and harmful bacteria on the scalp. It can also reduce scalp oil oxidation and synergize with the oil-controlling effect of propolis, making it suitable for oily dandruff. Salicylic acid has both antibacterial and keratin-regulating effects, dissolving excess keratin and unclogging hair follicles. At the same time, it promotes the penetration of small molecule active ingredients in propolis, enhancing the synergistic effect of anti-inflammatory and antibacterial properties, especially suitable for scalps with both oil and dandruff. Zinc pyrithione has a high inhibition rate against Malassezia furfur and good anti-inflammatory properties, which can relieve scalp inflammation caused by fungal stimulation. It complements the anti-inflammatory components of propolis, is mild and low-irritant, and suitable for sensitive scalps. The three ingredients are flexibly combined to ensure a strong inhibition of dandruff-causing bacteria, while also being well compatible with PEG-40 hydrogenated castor oil, surfactants, and other ingredients in the formula. This allows for precise targeting of different dandruff types (dry, oily, and sensitive), significantly improving the product's dandruff-removing durability and applicability.

[0013] As a further description of the above technical solution: the thickener is at least one of guar gum C-14S, hydroxyethyl cellulose, or xanthan gum. Guar gum C-14S, as a modified natural polymer, has excellent water solubility and excellent compatibility with propolis, PEG-40 hydrogenated castor oil, and anti-dandruff agents (such as selenium disulfide). After thickening, the texture is light and non-sticky, suitable for oily scalps, and can also help active ingredients adhere evenly to the scalp. Hydroxyethyl cellulose has outstanding stability and can stably disperse various components within a wide pH and temperature range, preventing propolis from precipitating due to system fluctuations. It is also mild and non-irritating, improving the smoothness of the product. Xanthan gum has strong salt resistance and can tolerate the electrolyte environment brought about by surfactants in the formula. A small amount can be added for efficient thickening, while also having a certain antibacterial auxiliary effect, synergistically with the antibacterial effect of propolis. The flexible combination of the three can ensure that the system consistency is appropriate (avoiding being too thin or too thick) without interfering with the activity of oil-controlling and anti-dandruff ingredients, further enhancing product stability and user experience, and adapting to various scalp care scenarios.

[0014] As a further description of the above technical solution: the pearlescent agent is ethylene glycol distearate (EGDS). EGDS has excellent compatibility with oil-phase components such as cetearyl alcohol and stearic acid in the formulation. It can be uniformly dispersed during heating at 80-85℃, and forms a delicate and stable pearlescent effect after cooling, enhancing the visual appeal of the product. At the same time, EGDS can enhance the viscosity and stability of the system, and works synergistically with thickeners such as guar gum C-14S and hydroxyethyl cellulose to avoid layering or sedimentation, ensuring uniform dispersion of active ingredients such as propolis and anti-dandruff agents. In addition, its low-irritant properties are compatible with ingredients such as propolis and moisturizers, and will not interfere with the oil-controlling and anti-dandruff effects. It can also improve the smoothness of the product during application, reduce rinsing residue, and further enhance the overall quality of the product and the user experience.

[0015] The present invention also provides a method for preparing the above-mentioned propolis shampoo, comprising the following steps: (1) Add pure water to the emulsification equipment, add disodium EDTA, polyquaternium-10 and thickener under stirring, stir evenly and then heat to 35-45℃, add anionic surfactant, keep warm and stir until dissolved; (2) Add cetearyl alcohol, stearic acid, honey and pearlescent agent, heat to 80-85°C and stir until dissolved; (3) Add the premix of propolis and PEG-40 hydrogenated castor oil, stir and mix well, then add the premix of moisturizer and anti-dandruff agent, and stir for 20-40 minutes. (4) After vacuuming and degassing, cool down to 45-55°C, add KF-982 and amphoteric surfactant, and stir for 3-8 minutes; when cooled to 35-45°C, adjust the pH to 4.5-5.5 with an aqueous solution of citric acid; (5) Continue stirring until the temperature drops to 30-40℃, and let stand for 12-36 hours to obtain the product.

[0016] The manufacturing method of this invention employs staged temperature control (dissolving anionic surfactants at 35–45°C and dispersing oil phase components at 80–85°C) to ensure the full dissolution of oil phase components such as cetearyl alcohol and stearic acid while avoiding high-temperature damage to the active ingredients of propolis. Premixing propolis with PEG-40 hydrogenated castor oil and premixing the moisturizer with the anti-dandruff agent ensures uniform dispersion of poorly soluble components, solving the problems of poor propolis solubility and easy aggregation. Vacuum degassing effectively removes air bubbles from the system, reducing the risk of oxidation and improving the uniformity of the product texture. Staged cooling and addition of KF-982 and amphoteric surfactants, along with precise pH adjustment to 4.5–5.5, protects the activity of heat-sensitive components and adapts to the slightly acidic environment of the scalp, enhancing gentleness. Finally, static settling further stabilizes the system and prevents stratification. The overall process design is scientific, balancing component stability, activity retention, and production feasibility, ensuring the product's high efficiency in oil control and dandruff removal, and is suitable for large-scale production.

[0017] As a further description of the above technical solution: the vacuuming and exhausting operation in step (4) is as follows: each time the vacuum is reduced to -0.06 to -0.08 MPa, it is maintained for 3 to 5 minutes and then restored to normal pressure. This cycle is repeated 2 to 4 times.

[0018] The system efficiently removes air bubbles (including microbubbles) through negative pressure, preventing the product from becoming loose due to residual bubbles. The circulating operation gradually removes air that was not completely removed during the initial vacuuming, minimizing the oxidative damage of free oxygen to the unsaturated active ingredients (such as flavonoids and phenolic acids) in propolis, thus ensuring the stability of efficacy. This negative pressure value is also compatible with the material temperature of 80-85℃, which avoids boiling of the aqueous phase caused by excessive negative pressure, and promotes the coalescence and discharge of bubbles through repeated pressure alternation, enhancing the system's compactness and further improving the sensory quality and efficacy reliability of the product.

[0019] Compared with the prior art, the beneficial effects of the present invention are: The propolis shampoo of this invention solves the problems of poor solubility and easy oxidation of propolis in the prior art by premixing propolis with PEG-40 hydrogenated castor oil in an appropriate ratio, thus ensuring the stability of its active ingredients (such as flavonoids and phenolic acids). The synergistic effect of amphoteric surfactants and anionic surfactants balances cleansing power and gentleness, overcoming the defects of strong irritation in traditional formulas. The targeted compounding of moisturizers and anti-dandruff agents not only avoids the imbalance of "dry outside and oily inside" but also enhances the oil control and anti-dandruff effect through synergistic effect with the anti-inflammatory and antibacterial effects of propolis, solving the problem of limited efficacy of single ingredients. The combination of thickeners and pearlescent agents improves the stability of the system while optimizing the texture and sensory experience, avoiding the drawbacks of easy layering and sticky residue in traditional formulas. The manufacturing method of this invention ensures full dissolution of components through staged temperature control while minimizing the damage to propolis activity caused by high temperatures; the step-by-step addition of premixes ensures uniform dispersion of poorly soluble components; vacuum degassing effectively removes bubbles and reduces oxidation, improving product texture uniformity; and staged cooling is used to add heat-sensitive components and precisely adjust the pH to a slightly acidic level to suit the scalp environment, enhancing gentleness and stability. The overall manufacturing method balances activity preservation and production feasibility, providing reliable support for large-scale production and significantly improving the overall quality of the product. Detailed Implementation

[0020] The claims of the present invention will be further described in detail below with reference to specific embodiments, but this does not constitute any limitation on the present invention. Any limited modifications made by any person within the scope of protection of the claims of the present invention are still within the scope of protection of the claims of the present invention.

[0021] It is worth noting that, unless otherwise specified, all components used in this invention are materials existing in the art and can be obtained through commercial channels or prepared by existing methods.

[0022] Example 1 This embodiment provides a propolis shampoo comprising the following components by weight percentage: 67.15% pure water, 0.1% disodium EDTA, 0.15% polyquaternium-10, 3% AES, 10% K12A, 2% AES, 2% cetearyl alcohol, 1% stearic acid, 2% honey, 0.5% propolis, 2.5% PEG-40 hydrogenated castor oil, 1.2% glycerin, 0.2% selenium disulfide, 0.8% guar gum C-14S, 2% CAB-35, 4% EGDS, 0.1% KF-982, 0.2% citric acid, 0.6% hydroxyethyl cellulose, and 0.5% Adolf mood fragrance.

[0023] Its production method includes the following steps: (1) Add pure water to the emulsification equipment, turn on the homogenizer, set the speed to 20 r / min, add disodium EDTA, polyquaternium-10, guar gum C-14S and hydroxyethyl cellulose, stir evenly and start heating, raise the temperature to 40℃, add anionic surfactants AESA, K12A and AES, keep at 40℃, stir for 1 hour until completely dissolved. (2) Add cetearyl alcohol, stearic acid, honey and EGDS, turn on the heat and heat to 82°C, stirring (20 r / min) until dissolved; (3) Add the premix of propolis and PEG-40 hydrogenated castor oil, stir (20 r / min) for about 10 min until well mixed, then add the premix of glycerol and selenium disulfide (homogenized in advance), stir at 20 r / min for about 30 minutes. (4) Vacuum the pressure from atmospheric pressure down to -0.07 MPa, maintain for 3 minutes and then restore to normal pressure. Repeat this cycle 3 times. When the temperature reaches 50°C, add KF-982, CAB-35 and Adolf Mood Fragrance, and stir at 30 r / min for 5 minutes. Stop cooling when the temperature drops to 40°C and adjust the pH to 5.0 (weakly acidic) with citric acid. (5) Continue stirring until the temperature naturally cools to 35°C, then stop stirring and let stand for 24 hours to obtain propolis shampoo (light brown emulsion), which is then packaged (120 mL / bottle).

[0024] Example 2 This embodiment provides a propolis shampoo comprising the following components by weight percentage: 65% pure water, 0.05% disodium EDTA, 0.1% polyquaternium-10, 5% AES, 6% K12A, 5% AES, 3% cetearyl alcohol, 1.3% stearic acid, 4% honey, 0.7% propolis, 2% PEG-40 hydrogenated castor oil, 0.5% butylene glycol, 0.5% oat β-glucan, 0.3% salicylic acid, 1% guar gum C-14S, 1% hydroxyethyl cellulose, 1% CAB-35, 3% EGDS, 0.05% KF-982, 0.1% citric acid, and 0.4% rose fragrance.

[0025] Its production method includes the following steps: (1) Add pure water to the emulsification equipment, turn on the homogenizer, set the speed to 20 r / min, add disodium EDTA, polyquaternium-10, guar gum C-14S and hydroxyethyl cellulose, stir evenly and heat to 35℃, add AESA, K12A and AES, keep warm and stir for 40 minutes until dissolved. (2) Add cetearyl alcohol, stearic acid, honey and EGDS, heat to 80°C and stir (20 r / min) until dissolved; (3) Add the premix of propolis and PEG-40 hydrogenated castor oil, stir for 10 minutes to mix well, then add the premix of butylene glycol, oat β-glucan and salicylic acid, and stir at 20 r / min for 20 minutes. (4) Vacuum the pressure down to -0.06 MPa, maintain it for 5 minutes and then restore it to normal pressure. Repeat this process twice. When the pressure is cooled to 45°C, add KF-982, CAB-35 and rose essence, and stir at 30 r / min for 3 minutes. When the pressure is cooled to 35°C, adjust the pH to 4.5 with citric acid. (5) Continue stirring until the temperature drops to 30°C, let stand for 16 hours to obtain propolis shampoo (light brown emulsion), and dispense (120 mL / bottle).

[0026] Example 3 This embodiment provides a propolis shampoo comprising the following components by weight percentage: 64.4% pure water, 0.2% disodium EDTA, 0.3% polyquaternium-10, 6% AES, 8% K12A, 4% AES, 1% cetearyl alcohol, 2% stearic acid, 1% honey, 0.3% propolis, 3% PEG-40 hydrogenated castor oil, 2% sodium PCA, 0.5% zinc pyrithione (ZPT), 2% xanthan gum, 1% CAB-35, 3.5% EGDS, 0.2% KF-982, 0.3% citric acid, and 0.3% lavender fragrance.

[0027] Its production method includes the following steps: (1) Add pure water to the emulsification equipment, turn on the homogenizer, set the speed to 25 r / min, add disodium EDTA, polyquaternium-10, and xanthan gum, stir evenly and heat to 35℃, add AESA, K12A, and AES, keep warm and stir for 40 minutes until dissolved. (2) Add cetearyl alcohol, stearic acid, honey and EGDS, heat to 80°C and stir (20 r / min) until dissolved; (3) Add the premix of propolis and PEG-40 hydrogenated castor oil, stir for 10 minutes to mix well, then add the premix of sodium PCA and zinc pyrithione, and stir at 20 r / min for 20 minutes. (4) Vacuum the pressure down to -0.06 MPa, maintain it for 5 minutes and then restore it to normal pressure. Repeat this process twice. When the pressure is cooled to 45°C, add KF-982, CAB-35 and lavender essence, and stir at 30 r / min for 3 minutes. When the pressure is cooled to 35°C, adjust the pH to 4.5 with citric acid. (5) Continue stirring until the temperature drops to 30°C, let stand for 16 hours to obtain propolis shampoo (light brown emulsion), and dispense (120 mL / bottle).

[0028] Oil control efficacy test: 1. Experimental Objective and Principle: Sebum secretion is closely related to sebaceous gland function, which in turn is closely related to androgen regulation. 5α-reductase is a membrane protease located on microsomes and the cell nucleus. Using the reduced coenzyme NADPH as a hydrogen donor, it catalyzes the conversion of testosterone to dihydrotestosterone (DHT), which induces excessive sebum secretion from the sebaceous glands. By inhibiting 5α-reductase activity, reducing DHT production, and lowering sebaceous gland lipid secretion levels, an oil-controlling effect can be achieved.

[0029] 2. Experimental Method: 20 µL of each of the propolis shampoo samples from Examples 1, 2, and 3 (sample 1, sample 2, sample 3) and physiological saline (negative control) were added to the enzyme reaction system for catalysis. The absorbance (Abs) was measured at 340 nm using a UV spectrophotometer (each sample was tested 3 times). The average absorbance was used to calculate the enzyme activity of the sample (sample enzyme activity = negative control enzyme activity × mean sample Abs / mean negative control Abs).

[0030] 3. Experimental results: The enzyme activity results of 5α-reductase are shown in Table 1. The enzyme activity of the negative control (physiological saline) is much greater than that of the three experimental samples, indicating that the propolis shampoo of the present invention has certain enzyme inhibitory activity and significant effect, proving that the propolis shampoo has a certain oil control effect.

[0031] Table 15 Results of α-reductase activity

[0032] Anti-dandruff efficacy test: 1. Purpose and principle of the experiment: Malassezia furfur secretes esterases. Esterases decompose lipids, leading to the production of inflammatory mediators such as prostaglandins, which in turn causes an inflammatory response. This further damages the scalp barrier, causing a large number of keratinocytes to shed abnormally and produce dandruff. Inhibiting the proliferation of Malassezia furfur can achieve the effect of removing dandruff.

[0033] 2. Test strain: Malassezia furfur (ATCC44344), provided by Shanghai Fuxiang Biotechnology Co., Ltd.

[0034] 3. Test Method: Referring to WS / T 650-2019 "Evaluation Methods for Antibacterial and Bacteriostatic Effects" 5.1.1 "Quantitative Suspension Inhibition Test": First, the test strain was cultured on an slant to obtain 1×10⁻⁶... 4 ~9×10 4 CFU / mL Malassezia furfur suspension; 30g of the propolis shampoo from Example 1 (sample 1), propolis shampoo from Example 2 (sample 2), and propolis shampoo from Example 3 (sample 3) stock solutions (simulating the contact concentration between the sample and the scalp during actual shampooing) and sterile water (negative control group) were taken and reacted with 0.6mL of Malassezia furfur suspension for 4 minutes (matching the average contact time during actual shampooing). The samples were inoculated in parallel into 4 petri dishes, culture medium was poured in, and the dishes were incubated in a 36℃ biochemical incubator for 5 days. Each sample was repeated three times. The number of viable bacteria was counted, and the inhibition rate was calculated as follows: Inhibition rate = (number of viable bacteria in the control group - number of viable bacteria in the experimental group) / number of viable bacteria in the control group × 100%).

[0035] 4. Test Results The results of the antibacterial rate of Malassezia furfur are shown in Table 2: The propolis shampoos of Examples 1, 2 and 3 all showed an antibacterial rate of >99.9% against Malassezia furfur, which is far higher than the 50% judgment standard. This indicates that the propolis shampoo of the present invention can inhibit the proliferation of Malassezia furfur and has a highly efficient and stable dandruff removal effect.

[0036] Table 2 Results of the antibacterial rate of Malassezia furfur.

[0037] This invention's propolis shampoo combines oil control and dandruff removal: propolis works synergistically with dandruff removers such as selenium sulfide, salicylic acid, and zinc pyrithione to achieve an antibacterial rate of over 99.9% against Malassezia furfur. It effectively eradicates dandruff-causing bacteria and relieves scalp itching by leveraging the anti-inflammatory properties of propolis, thus blocking the dandruff regeneration cycle. Simultaneously, it targets and inhibits 5α-reductase activity (the enzyme activity of the sample was reduced by 12.8%~22.6% compared to the negative control), reducing dihydrotestosterone production and decreasing oil production from the source of sebaceous gland secretion. This forms a synergistic closed loop of oil control and antibacterial nutrition, dandruff removal and anti-inflammatory secretion stabilization, continuously reshaping the scalp's microecological balance and achieving dual high efficiency of thorough dandruff removal and long-lasting oil control.

[0038] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit them; those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features; and these modifications or substitutions will not cause the essence of the corresponding technical solutions to deviate from the protection scope of the present invention.

Claims

1. A propolis shampoo, characterized in that, It comprises the following components by weight percentage: 64-68% pure water, 0.05-0.2% disodium EDTA, 0.1-0.3% polyquaternium-10, 15-18% anionic surfactant, 1-3% cetearyl alcohol, 1-2% stearic acid, 1-4% honey, 0.3-0.7% propolis, 2-3% PEG-40 hydrogenated castor oil, 1-2% humectant, 0.2-0.5% anti-dandruff agent, 1-2% thickener, 1-2% amphoteric surfactant, 3-4% pearlescent agent, 0.05-0.2% KF-982, 0.1-0.3% citric acid, and 0.3-0.5% fragrance.

2. The propolis shampoo according to claim 1, characterized in that: The anionic surfactants include AESA, K12A, and AES.

3. The propolis shampoo according to claim 2, characterized in that: The amphoteric surfactant is CAB-35.

4. The propolis shampoo according to claim 1, characterized in that: The moisturizer is at least one of glycerin, butylene glycol, oat beta-glucan, or sodium PCA.

5. The propolis shampoo according to claim 1, characterized in that: The dandruff remover is at least one of selenium disulfide, salicylic acid, or zinc pyrithione.

6. The propolis shampoo according to claim 1, characterized in that: The thickener is at least one of guar gum C-14S, hydroxyethyl cellulose, or xanthan gum.

7. The propolis shampoo according to claim 1, characterized in that: The pearlescent agent is ethylene glycol distearate.

8. A method for preparing the propolis shampoo according to any one of claims 1-7, characterized in that, Includes the following steps: (1) Add pure water to the emulsification equipment, add disodium EDTA, polyquaternium-10 and thickener under stirring, stir evenly and then heat to 35-45℃, add anionic surfactant, keep warm and stir until dissolved; (2) Add cetearyl alcohol, stearic acid, honey and pearlescent agent, heat to 80-85°C and stir until dissolved; (3) Add the premix of propolis and PEG-40 hydrogenated castor oil, stir and mix well, then add the premix of moisturizer and anti-dandruff agent, and stir for 20-40 minutes. (4) After vacuuming and degassing, add KF-982, amphoteric surfactant and fragrance when cooled to 45-55℃, and stir for 3-8 minutes; when cooled to 35-45℃, adjust the pH to 4.5-5.5 with citric acid; (5) Continue stirring until the temperature drops to 30-40℃, and let stand for 12-36 hours to obtain the product.

9. The method for preparing propolis shampoo according to claim 8, characterized in that: The vacuuming and exhausting operation in step (4) is as follows: each time the vacuum is evacuated from atmospheric pressure to -0.06 to -0.08 MPa, maintained for 3 to 5 minutes and then restored to normal pressure, and the operation is repeated 2 to 4 times.

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