Pichia pastoris engineering bacteria with high yield of 11α-hydroxycanrenone and application thereof
By co-expressing SOD and/or Cat enzymes in Pichia pastoris, the problem of excessive oxidation of 11α-hydroxycanrenone in Pichia pastoris was solved, significantly improving the product yield and making it suitable for large-scale industrial production.
CN122128120APending Publication Date: 2026-06-02TIANJIN UNIV OF SCI & TECH +1
View PDF 0 Cites 0 Cited by
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- TIANJIN UNIV OF SCI & TECH
- Filing Date
- 2026-03-18
- Publication Date
- 2026-06-02
AI Technical Summary
Technical Problem
When Pichia pastoris heterologously expresses CYP68J5 to convert canrone, 11α-hydroxycanrone is easily over-oxidized, resulting in extremely low yield, which is difficult to meet the needs of efficient industrial production.
Method used
Superoxide dismutase (SOD) and/or catalase (Cat) were co-expressed in Pichia pastoris, which reduced intracellular peroxidation levels and inhibited the excessive oxidative degradation of 11α-hydroxycanrenone.
Benefits of technology
The yield of 11α-hydroxycanrenone was significantly improved. The yield of engineered strain GS115-CYP68J5-SOD increased to 43%, the yield of GS115-CYP68J5-Cat increased to 71%, and the yield of GS115-CYP68J5-SOD-Cat increased significantly to 82%.
✦ Generated by Eureka AI based on patent content.
Smart Images
Figure CN122128120A_ABST
Abstract
This invention discloses a high-yield Pichia pastoris engineered strain of 11α-hydroxycanrenone and its applications, belonging to the fields of genetic engineering and biocatalysis. Using Pichia pastoris GS115 as the host, and based on the heterologous expression of steroidal 11α-hydroxylase CYP68J5, superoxide dismutase (SOD) and / or catalase (Cat) were overexpressed to construct recombinant engineered strains GS115-CYP68J5-SOD, GS115-CYP68J5-Cat, and GS115-CYP68J5-SOD-Cat. By reducing intracellular peroxidation levels through antioxidant enzymes, the excessive oxidative degradation of the product 11α-hydroxycanrenone was inhibited. The engineered strain co-expressing SOD and Cat achieved a product yield of 82%, which was 5.1 times higher than the control group. The engineered strain constructed in this invention exhibits high transformation efficiency and excellent product yield. The process is simple and easily industrialized, and can be widely applied to the efficient biosynthesis of 11α-hydroxycanrenone.
Need to check novelty before this filing date? Find Prior Art