A vitamin d-ovalbumin peptide composition, preparation method and application

Abstract

The application discloses a vitamin D-ovalbumin peptide composition, which comprises vitamin D3, ovalbumin peptide, a water-soluble auxiliary solubilizing stabilizer, an isotonic buffer regulator, an anti-oxidation bacteriostatic agent and a solvent in a residual amount, wherein the solvent is water. Compared with the prior art, the application has the advantages that a vitamin D-ovalbumin peptide composition with high stability and synergistic effect can be widely used in food, health products and medicines, and a preparation method and application thereof are provided.

Description

Technical Field

[0001] This invention relates to the field of biomedical technology, specifically to a vitamin D-ovalbumin peptide composition, its preparation method, and its application. Background Technology

[0002] Vitamin D3 is an essential fat-soluble vitamin that plays a key role in calcium absorption and bone metabolism regulation. It can effectively promote the absorption and utilization of calcium in the intestines, maintain normal bone mineralization, prevent and improve osteoporosis, and is also important for maintaining the body's immune function and regulating cell growth and differentiation.

[0003] However, vitamin D3 itself has problems with poor water solubility and instability. In particular, in aqueous systems, it is easily degraded by factors such as temperature, light, and oxygen, which leads to a decrease in its biological activity and greatly limits its application in the fields of food, health products and pharmaceuticals.

[0004] Oval albumin peptides are small molecule peptides obtained from oval albumin through enzymatic hydrolysis. They are rich in a variety of essential amino acids and are easily absorbed and utilized by the human body. They not only provide high-quality nutritional support for the body, but also have a variety of biological activities such as immune regulation, anti-inflammatory and antioxidant effects, and promotion of nutrient absorption. They are an ideal natural carrier material and nutritional supplement.

[0005] Existing technologies include compound preparations of vitamin D with proteins or peptides, but most of these preparations suffer from problems such as unreasonable component combinations, poor vitamin D stability, insufficient water solubility, and insignificant synergistic effects among the components. Furthermore, some preparations do not consider the antibacterial and antioxidant requirements during product storage, resulting in short shelf life and insufficient safety. At the same time, the preparation processes of existing preparations are often quite complex, with high production costs, making it difficult to achieve large-scale production and failing to meet the actual needs of scenarios such as osteoporosis prevention and treatment, postoperative and elderly nutritional support. Summary of the Invention

[0006] The technical problem to be solved by the present invention is to overcome the above-mentioned technical defects and provide a vitamin D-oval albumin peptide composition, preparation method and application with strong stability and good synergy, which can be widely used in food, health products and pharmaceuticals.

[0007] To solve the above-mentioned technical problems, the technical solution provided by the present invention is: a vitamin D-ovalbumin peptide composition, comprising vitamin D3, ovalbumin peptide, a water-soluble solubilizing stabilizer, an isotonic buffer regulator, an antioxidant and antibacterial agent, and the balance being a solvent, wherein the solvent is water.

[0008] Preferably, the vitamin D is vitamin D3; The ovalbumin peptide is a small molecule ovalbumin peptide prepared by complex enzymatic hydrolysis; The water-soluble solubilizing stabilizer is fructooligosaccharide; The antioxidant and antibacterial agent is zinc gluconate; The solvent also includes blueberry powder.

[0009] Preferably, the proportions of each component, by mass percentage, are as follows: Vitamin D 0.0005%-0.3%, ovalbumin peptide 2.0%-25%, water-soluble solubilizer and stabilizer 0.5%-3%, isotonic buffer regulator 0.5%-2%, antioxidant and antibacterial agent 0.05%-0.5%.

[0010] Another aspect of the present invention discloses a method for preparing a vitamin D-ovalbumin peptide composition, comprising the following steps: S1: Preparation of small molecule ovalbumin peptides based on a compound enzymatic hydrolysis method; S2: Prepare vitamin D3 premix solution; S3: The small molecule ovalbumin peptide obtained in S1 is mixed and homogenized with vitamin D3 premix, and water-soluble solubilizing stabilizer, isotonic buffer regulator, antioxidant and antibacterial agent and solvent are added and mixed to dissolve. S4: The finished formulation is prepared by fine filtration, sterilization or freeze drying.

[0011] Preferably, the finished formulation is an oral formulation or an injectable formulation.

[0012] Preferably, the sterilization in S4 is aseptic filtration or autoclaving.

[0013] Another aspect of the present invention discloses the application of a vitamin D-ovalbumin peptide composition in bone health regulation and nutritional supplementation.

[0014] Preferably, the application includes the preparation of foods, health products, or pharmaceuticals for the prevention or improvement of osteoporosis and the promotion of calcium absorption.

[0015] The advantages of this invention compared to existing technologies are as follows: The composition of this invention is scientifically formulated, using a complex enzymatically hydrolyzed small molecule ovalbumin peptide as a natural carrier to effectively enhance the synergistic activity of vitamin D3, thus providing both bone health regulation and nutritional supplementation. The combination of fructooligosaccharides, zinc gluconate, and blueberry powder further optimizes water solubility, stability, and nutritional value, ensuring product safety. The preparation process of this invention is mild, simple, low-cost, and easy to scale up for production. It can be widely used in the preparation of foods, health products, or pharmaceuticals that prevent or improve osteoporosis and promote calcium absorption, and has broad application prospects. 0<Attached Figure Descriptions Figure 1 This is a schematic diagram of the process flow for preparing a vitamin D-ovalbumin peptide composition. Detailed Implementation

[0016] The specific embodiments of the present invention will be further described below with reference to the accompanying drawings. Identical components are indicated by the same reference numerals.

[0017] It should be noted that the terms front, back, left, right, up, and down used in the following description refer to the directions in the attached diagram, while the terms inside and outside refer to the directions toward or away from the geometric center of a specific component, respectively.

[0018] To make the content of this invention easier to understand, the technical solutions in the embodiments of this invention will be clearly and completely described below with reference to the accompanying drawings.

[0019] Combined with appendix Figure 1 As shown, a vitamin D-ovalbumin peptide composition is characterized by comprising vitamin D3, ovalbumin peptide, a water-soluble solubilizing stabilizer, an isotonic buffer regulator, an antioxidant and antibacterial agent, and the balance being a solvent, wherein the solvent is water; Vitamin D is vitamin D3; The ovalbumin peptide is a small molecule ovalbumin peptide prepared by complex enzymatic hydrolysis; The water-soluble solubilizing stabilizer is fructooligosaccharide; The antioxidant and antibacterial agent is zinc gluconate; The solvent also includes blueberry powder.

[0020] When using, the proportions of each component, by mass percentage, are as follows: Vitamin D 0.0005%-0.3%, ovalbumin peptide 2.0%-25%, water-soluble solubilizer and stabilizer 0.5%-3%, isotonic buffer regulator 0.5%-2%, antioxidant and antibacterial agent 0.05%-0.5%.

[0021] Example 1 A vitamin D-ovalbumin peptide composition, comprising the following components by mass percentage: vitamin D3 0.0005%, ovalbumin peptide 2.0%, fructooligosaccharide 0.5%, isotonic buffer 0.5%, zinc gluconate 0.05%, with the remainder being water, and blueberry powder added to the water solvent.

[0022] Preparation method: S1: Prepare small molecule ovalbumin peptides based on compound enzymatic hydrolysis; S2: Take the corresponding amount of vitamin D3, add a small amount of water to dissolve, and prepare a premix; S3: Mix the small molecule ovalbumin peptides and vitamin D3 premix and homogenize, add fructooligosaccharides, isotonic buffer, zinc gluconate and water containing blueberry powder, and mix and dissolve; S4: After fine filtration and autoclaving, obtain an injectable formulation.

[0023] Example 2 A vitamin D-ovalbumin peptide composition, comprising the following components by mass percentage: vitamin D3 0.15%, ovalbumin peptide 13.5%, fructooligosaccharide 1.75%, isotonic buffer 1.25%, zinc gluconate 0.275%, with the remainder being water, and an appropriate amount of blueberry powder added to the water solvent.

[0024] Preparation method: S1: Prepare small molecule ovalbumin peptides based on compound enzymatic hydrolysis; S2: Take the corresponding amount of vitamin D3, add a small amount of water to dissolve, and prepare a premix; S3: Mix the small molecule ovalbumin peptides and vitamin D3 premix and homogenize, add fructooligosaccharides, isotonic buffer, zinc gluconate and water containing blueberry powder, and mix and dissolve; S4: After fine filtration, sterilization by aseptic filtration, and freeze drying, oral powder is obtained.

[0025] Example 3 A vitamin D-ovalbumin peptide composition, comprising the following components by mass percentage: vitamin D3 0.3%, ovalbumin peptide 25%, fructooligosaccharide 3%, isotonic buffer 2%, zinc gluconate 0.5%, with the remainder being water, and an appropriate amount of blueberry powder added to the water solvent.

[0026] Preparation method: S1: Prepare small molecule ovalbumin peptides based on compound enzymatic hydrolysis; S2: Take the corresponding amount of vitamin D3, add a small amount of water to dissolve, and prepare a premix; S3: Mix the small molecule ovalbumin peptides and vitamin D3 premix and homogenize, add fructooligosaccharides, isotonic buffer, zinc gluconate and water containing blueberry powder, and mix and dissolve; S4: After fine filtration and freeze drying, oral granules are obtained.

[0027] The working principle of this invention: This invention converts ovalbumin into small molecule peptides through compound enzymatic hydrolysis. These peptides act as natural carriers to encapsulate fat-soluble vitamin D3. Combined with the solubilizing effect of fructooligosaccharides, this solves the problem of poor water solubility of vitamin D3. Zinc gluconate plays an antioxidant and antibacterial role, reduces vitamin D3 degradation and ensures product safety, while blueberry powder provides nutritional supplementation and achieves synergistic benefits. During preparation, premix preparation and homogenization ensure uniform dispersion of each component, while fine filtration, sterilization, or freeze-drying processes guarantee product purity and stability. In the finished product, the components work synergistically: vitamin D3 promotes calcium absorption and regulates bone health, while small-molecule ovalbumin peptides supplement nutrition, achieving dual benefits.

[0028] The contents not described in detail in this specification are existing technologies known to those skilled in the art.

[0029] All standard parts used in this invention can be purchased from the market, and irregular parts can be customized according to the description and drawings. The specific connection methods of each part adopt conventional methods such as bolts, rivets, and welding that are mature in the prior art. The machinery, parts and equipment adopt conventional models in the prior art, and the circuit connection adopts conventional connection methods in the prior art, which will not be described in detail here. The contents not described in detail in this specification belong to the prior art known to those skilled in the art.

[0030] The present invention and its embodiments have been described above. This description is not restrictive, and the accompanying drawings are only one embodiment of the present invention; the actual structure is not limited thereto. In conclusion, if those skilled in the art are inspired by this description and design similar structures and embodiments without departing from the spirit of the invention, such designs should fall within the protection scope of the present invention.

Claims

1. A vitamin D-ovalbumin peptide composition, characterized in that: It includes vitamin D3, ovalbumin peptide, water-soluble solubilizing stabilizer, isotonic buffer regulator, antioxidant and antibacterial agent, and the balance being a solvent, wherein the solvent is water.

2. The vitamin D-ovalbumin peptide composition according to claim 1, characterized in that: The vitamin D mentioned is vitamin D3; The ovalbumin peptide is a small molecule ovalbumin peptide prepared by complex enzymatic hydrolysis; The water-soluble solubilizing stabilizer is fructooligosaccharide; The antioxidant and antibacterial agent is zinc gluconate; The solvent also includes blueberry powder.

3. The vitamin D-ovalbumin peptide composition according to claim 2, characterized in that: The proportions of each component, by mass percentage, are as follows: Vitamin D 0.0005%-0.3%, ovalbumin peptide 2.0%-25%, water-soluble solubilizer and stabilizer 0.5%-3%, isotonic buffer regulator 0.5%-2%, antioxidant and antibacterial agent 0.05%-0.5%.

4. A method for preparing the vitamin D-ovalbumin peptide composition as described in any one of claims 1-3, characterized in that: Includes the following steps: S1: Preparation of small molecule ovalbumin peptides based on a compound enzymatic hydrolysis method; S2: Prepare vitamin D3 premix solution; S3: The small molecule ovalbumin peptide obtained in S1 is mixed and homogenized with vitamin D3 premix, and water-soluble solubilizing stabilizer, isotonic buffer regulator, antioxidant and antibacterial agent and solvent are added and mixed to dissolve. S4: The finished formulation is prepared by fine filtration, sterilization or freeze drying.

5. The vitamin D-ovalbumin peptide composition according to claim 4, characterized in that: The finished formulation is an oral formulation or an injectable formulation.

6. The vitamin D-ovalbumin peptide composition according to claim 4, characterized in that: The sterilization in S4 is either aseptic filtration or autoclaving.

7. The use of a vitamin D-ovalbumin peptide composition as described in any one of claims 1-3 in bone health regulation and nutritional supplementation.

8. The application according to claim 7, characterized in that: The applications include the preparation of foods, health products, or medicines for the prevention or improvement of osteoporosis and the promotion of calcium absorption.

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