A sealed high-efficiency tissue shearing device for chicken embryo primary cell extraction

Abstract

This application relates to the field of biological tissue processing equipment technology, and discloses a closed-loop, high-efficiency tissue mincing device for extracting primary cells from chicken embryos. The device mainly includes a support frame, a cup body, a cooling box, a shearing assembly, and a sealing assembly. The shearing assembly is driven by magnetic coupling, with an external motor rotating a disk, which in turn drives a magnet at the bottom of the cup body to rotate a rotating shaft and moving blades. This creates an alternating shearing action with stationary blades fixed to the inner wall of the cup body, achieving uniform and efficient physical fragmentation of the chicken embryo tissue. The cooling assembly includes a cooling jacket surrounding the cup body and a circulating cooling system, effectively removing heat generated during the shearing process. The sealing assembly consists of a top cover, a sealing gasket, and a feed pipe with a sealing plug, ensuring a completely sealed operation. This invention solves the problems of significant mechanical damage to cells, easy thermal inactivation of samples, and the risk of contamination during open operation in existing technologies. It has the advantages of good cell morphology preservation, high biosafety, and convenient operation.

Description

Technical Field

[0001] This invention relates to the field of biological tissue processing equipment technology, specifically a closed-type high-efficiency tissue mincing device for the extraction of primary cells from chicken embryos. Background Technology

[0002] Chicken embryo tissue is an important source of experimental materials in fields such as biomedical research, vaccine preparation, and cell culture. Current techniques for processing chicken embryo tissue typically rely on traditional manual methods or open-type mixing devices, which have significant technical limitations.

[0003] Manual cell shredding is inefficient and inconsistent, with difficulty in controlling shearing force, easily causing excessive mechanical damage to cells, leading to morphological destruction and reduced activity, directly affecting the reliability of subsequent experimental results. While conventional mechanical stirring or shredding equipment can improve efficiency, its open design allows tissue droplets and aerosols to easily splash during operation, causing sample loss and cross-contamination, and posing biosafety risks to laboratory personnel. In addition, the frictional heat generated by continuous operation of mechanical devices can raise the local temperature of the sample, causing thermal damage to heat-sensitive cells and tissue components. Furthermore, the cleaning, disassembly, and assembly of existing equipment are often cumbersome, failing to meet the urgent needs of laboratories for efficient, standardized, and pollution-free operating procedures. Summary of the Invention

[0004] To address the shortcomings of existing technologies, this invention provides a closed, high-efficiency tissue mincing device for primary cell extraction from chicken embryos, which solves the problems of significant mechanical damage to cells, easy contamination during open operation, sample thermal inactivation, and low efficiency in existing chicken embryo tissue mincing processes.

[0005] To achieve the above objectives, the present invention provides the following technical solution: a closed-type high-efficiency tissue mincing device for primary cell extraction from chicken embryos, comprising a support frame, an installation groove in the upper middle part of the support frame, a cup body slidably connected inside the installation groove, a cooling box fixedly connected to the lower part of the support frame, a liquid guide pipe fixedly connected to the upper left side of the cooling box, a connecting sleeve fixedly connected to the upper outer side of the liquid guide pipe, an infusion pump fixedly connected to the upper right side of the cooling box, a suction pipe fixedly connected to the input end of the infusion pump, an infusion pipe fixedly connected to the output end of the infusion pump, a threaded sleeve threadedly connected to the outer side of the end of the infusion pipe away from the infusion pump, a control panel fixedly connected to the upper front side of the support frame, a cooler fixedly connected to the lower front side of the support frame, a shearing component inside the cup body for shearing chicken embryo tissue, a cooling component outside the cup body for cooling the cup body, and a sealing component on the upper part of the cup body for sealing the cup body.

[0006] Preferably, the shearing assembly includes a magnet disposed at the bottom of the cup body, a rotating shaft fixedly connected to the upper part of the magnet, movable blades evenly fixedly connected to the outer periphery of the rotating shaft, a hanging ring fixedly connected to the upper part of the rotating shaft, stationary blades evenly fixedly connected to the lower periphery of the inner wall of the cup body, a waterproof box fixedly connected to the bottom of the cooling box, a small motor fixedly connected to the bottom of the waterproof box, a turntable fixedly connected to the output end of the small motor, and a disk fixedly connected to the upper part of the turntable.

[0007] Preferably, the cooling assembly includes a cooling sleeve, which is fixedly connected to the outside of the cup body. A cooling cavity is provided on the side of the cooling sleeve opposite to the cup body. A drain pipe is fixedly connected to the lower left side of the cooling sleeve, and an inlet pipe is fixedly connected to the upper right side of the cooling sleeve.

[0008] Preferably, the sealing assembly includes a top cover, which is disposed on the upper outer side of the cup body. A sealing gasket is fixedly connected to the bottom of the top cover, and a feed tube is fixedly connected to the upper right side of the top cover. A sealing plug is slidably connected inside the feed tube.

[0009] Preferably, the lower left and right sides of the outer side of the cup body are fixedly connected to limit blocks, and the upper left and right sides of the support frame are provided with limit grooves.

[0010] Preferably, the lower part of the cooling tank is fixedly connected to support pads around its perimeter, and the end of the liquid extraction pipe away from the infusion pump is fixedly connected to the cooling tank.

[0011] Preferably, the magnet is connected to the disk by magnetic force, and the moving blade and the stationary blade form an interleaved structure.

[0012] Preferably, the end of the drain pipe away from the cooling sleeve is slidably connected to the inner side of the upper part of the connecting sleeve, and the threaded sleeve is threadedly connected to the inlet pipe.

[0013] Preferably, the top cover is made of stainless steel, and the sealing plug and sealing gasket are both made of silicone rubber.

[0014] Preferably, the limiting block is slidably connected inside the limiting groove.

[0015] Working Principle: When a sealed processing of chicken embryo tissue is required, push the sealing plug to slide it into the feed pipe, then add the peeled chicken embryo tissue into the cup body. Rotate the top cover to install it on the upper outer side of the cup body. The top cover is made of stainless steel to ensure strength and corrosion resistance. A sealing gasket fills the connection between the cup body and the top cover to ensure a tight seal. Silicone rubber ensures the elasticity and sealing performance of the sealing plug and gasket. Push the cooling jacket to move the inlet and outlet pipes, allowing the outlet pipe to slide into the connecting sleeve. Then rotate the threaded sleeve to connect the inlet and outlet pipes. Start the infusion pump to draw coolant into the pump. The coolant is then fed into the cooling chamber through the outlet and inlet pipes to cool the cup body. Finally, the coolant is guided into the cooling tank through the outlet and guide pipes for recooling. This achieves efficient shredding and processing. The cooling system effectively counteracts the heat generated by the small motor's rotation and the blade's shearing action. When chicken embryo tissue needs to be minced, it is added to the cup. The small motor is activated via the control panel, causing the turntable to rotate. The turntable drives the magnetic disk, which in turn drives the magnet to rotate. The magnet drives the rotating shaft, which in turn drives the moving blade. The alternating movement of the moving and stationary blades minces the chicken embryo tissue, achieving a highly efficient mincing device. This device breaks down the tissue using shearing force, preserving the integrity of cell morphology to the greatest extent and reducing mechanical damage. After mincing the chicken embryo tissue, the sealing plug is pulled to detach it from the feed tube, and the tissue suspension is directly aspirated through a large-aperture pipette for subsequent digestion steps. This highly efficient mincing device allows for sealed processing of the chicken embryo tissue, preventing external aerosol contamination and the splashing of tissue fragments.

[0016] This invention provides a closed, high-efficiency tissue mincing device for primary cell extraction from chicken embryos. It offers the following advantages: 1. This invention abandons the high-speed impact grinding principle of traditional homogenizers or pulverizers. Instead, it uses magnetic force to drive moving and stationary blades to form a precise alternating shearing motion, simulating and replacing the surgical operation of artificial ophthalmic scissors. This device can precisely cut tissue into uniform tissue fragments of about 1 mm³, rather than a paste-like homogenate. This physical state maximizes the contact area of ​​subsequent digestive enzymes, improving digestion efficiency, while avoiding cell rupture and inactivation caused by excessive mechanical shearing, thereby significantly improving the yield and viability of the final primary live cells.

[0017] 2. Through optimized blade gap and cup fluid design, this invention is not only suitable for the tiny chicken embryo heart, but also widely applicable to the processing of various soft tissues such as the heart, liver, spleen, lungs, kidneys and brain of chicken embryos at different developmental stages. Whether it is the brittle liver or the fibrous muscle tissue, it can achieve efficient shearing, solving the problem of poor processing effect of existing equipment on trace or specific texture samples, and meeting the needs of cell biology and molecular biology research for obtaining various types of tissues.

[0018] 3. This invention integrates a circulating cooling system consisting of a cooling jacket, a cooling box, an infusion pump, and a refrigerator, which effectively counteracts the heat generated by motor rotation and mechanical shearing. During the shearing process, the tissue block is always kept in a low-temperature environment, which effectively inhibits the activity and metabolic rate of endogenous enzymes in the tissue and prevents heat-sensitive cells from being damaged during the physical processing stage.

[0019] 4. This invention uses a sealing assembly consisting of a stainless steel top cover, a silicone rubber sealing gasket, and a sealing plug to completely seal the cup during operation, effectively preventing the diffusion of harmful aerosols and the entry of external contaminants. This not only provides biosafety protection for operators but also provides a sterile processing environment for the originally sterile chicken embryo tissue, reducing the risk of contamination in primary cell culture.

[0020] 5. This invention adopts a magnetically driven shaftless design, which makes the inside of the cup smooth and without dead corners. Combined with a one-button self-cleaning mode, and the characteristics of the cup and blade assembly being detachable and resistant to high temperature and high pressure sterilization, it greatly simplifies the cleaning process between batch processing, ensures zero cross-contamination, and is particularly suitable for continuous preparation of large numbers of samples in the laboratory. Attached Figure Description

[0021] Figure 1 This is a perspective view of the present invention; Figure 2 This is a right view of the present invention; Figure 3 This is the left view of the present invention; Figure 4 This is a top view of the present invention; Figure 5 This is a cross-sectional view of the cup body of the present invention; Figure 6 This is a cross-sectional view of the cooling box of the present invention; Figure 7 for Figure 3 Enlarged view of point A in the image; Figure 8 for Figure 5 Enlarged view of point B in the image.

[0022] The components are as follows: 1. Support frame; 2. Mounting groove; 3. Cup body; 4. Top cover; 5. Feed pipe; 6. Sealing plug; 7. Sealing gasket; 8. Cooling jacket; 9. Cooling chamber; 10. Cooling box; 11. Support pad; 12. Refrigerator; 13. Control panel; 14. Liquid inlet pipe; 15. Liquid outlet pipe; 16. Liquid guide pipe; 17. Connecting sleeve; 18. Infusion pump; 19. Liquid extraction pipe; 20. Infusion pipe; 21. Limiting groove; 22. Waterproof box; 23. Small motor; 24. Turntable; 25. Disk; 26. Limiting block; 27. Stationary blade; 28. Magnet; 29. ​​Rotating shaft; 30. Moving blade; 31. Lifting ring; 32. Threaded sleeve. Detailed Implementation

[0023] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0024] Example: Please see the appendix Figure 1 - Appendix Figure 8This invention provides a closed-type high-efficiency tissue mincing device for primary chicken embryo cell extraction, comprising a support frame 1, an installation groove 2 in the upper middle part of the support frame 1, a cup body 3 slidably connected inside the installation groove 2, a cooling box 10 fixedly connected to the lower part of the support frame 1, a liquid guide tube 16 fixedly connected to the upper left side of the cooling box 10, a connecting sleeve 17 fixedly connected to the upper outer side of the liquid guide tube 16, an infusion pump 18 fixedly connected to the upper right side of the cooling box 10, a suction tube 19 fixedly connected to the input end of the infusion pump 18, an infusion tube 20 fixedly connected to the output end of the infusion pump 18, a threaded sleeve 32 threadedly connected to the outer side of the end of the infusion tube 20 away from the infusion pump 18, a control panel 13 fixedly connected to the upper front side of the support frame 1, a cooler 12 fixedly connected to the lower front side of the support frame 1, and limit blocks 26 fixedly connected to the lower outer sides of the cup body 3 on both sides. Limit grooves 21 are opened on both the left and right sides of the upper part of the support frame 1, and the limit blocks 26 slide. Connected inside the limiting groove 21, the lower part of the cooling box 10 is fixedly connected to the support pads 11. The end of the liquid extraction pipe 19 away from the infusion pump 18 is fixedly connected to the cooling box 10. The cup body 3 is equipped with a shearing component for shearing chicken embryo tissue. The shearing component includes a magnet 28, which is located at the bottom of the cup body 3. A rotating shaft 29 is fixedly connected to the upper part of the magnet 28. Moving blades 30 are evenly fixedly connected to the outer periphery of the rotating shaft 29. A hanging ring 31 is fixedly connected to the upper part of the rotating shaft 29 for easy maintenance of the blades. Stationary blades 27 are evenly fixedly connected to the lower periphery of the inner wall of the cup body 3. A waterproof box 22 is fixedly connected to the bottom of the cooling box 10. A small motor 23 is fixedly connected to the bottom of the waterproof box 22. A turntable 24 is fixedly connected to the output end of the small motor 23. A disk 25 is fixedly connected to the upper part of the turntable 24. The magnet 28 is connected to the disk 25 by magnetic force. The moving blades 30 and stationary blades 27 form an interlaced structure.

[0025] When chicken embryo tissue needs to be minced, the chicken embryo tissue is added to the cup body 3. The small motor 23 is started by the control panel 13 to drive the turntable 24 to rotate. The turntable 24 drives the disk 25 to rotate. The disk 25 drives the magnet 28 to rotate through magnetic force. The magnet 28 drives the rotating shaft 29 to rotate. The rotating shaft 29 drives the moving blade 30 to rotate. The moving blade 30 and the stationary blade 27 alternately mince the chicken embryo tissue. This achieves a highly efficient mincing device that can break the tissue with shearing force, preserve the integrity of cell morphology to the greatest extent, and reduce mechanical damage.

[0026] To adapt to the operating environment of a clean bench, the device adopts a compact design in a preferred embodiment of the present invention. The overall external dimensions of the support frame 1 together with the cooling box 10 are: length 180mm × width 160mm × height 260mm. This volume occupies only a small operating area of ​​the clean bench, and its height is much lower than the opening height of the clean bench's glass baffle, facilitating the placement or removal of the entire device by the experimenter without obstructing airflow. Simultaneously, the cup body 3 is designed with a slender structure, with an inner diameter of 40mm and an effective volume of 50mL. The smaller inner diameter design is suitable for micro-samples (e.g., 0.5g tissue + 2mL liquid), ensuring that the liquid level completely covers the blade assembly, preventing sample dispersion due to an excessively large cup bottom area, thus avoiding the inability to cut the sample.

[0027] In view of the small size and soft texture of chicken embryo tissue, in order to prevent tissue blocks from escaping or spinning with the liquid in the blade gap, the horizontal shearing gap between the outer edge of the moving blade 30 and the inner edge of the stationary blade 27 of the shearing assembly is precisely machined and controlled between 0.15mm and 0.3mm. This gap is much smaller than the minimum diameter of the chicken embryo tissue block, about 2mm, ensuring that each piece of tissue falling into the gap can be effectively cut off, rather than being squeezed through.

[0028] Please see the appendix Figure 1 - Appendix Figure 7 A cooling assembly is provided on the outside of the cup body 3 for cooling the cup body 3. The cooling assembly includes a cooling sleeve 8, which is fixedly connected to the outside of the cup body 3. A cooling chamber 9 is opened on the side of the cooling sleeve 8 opposite to the cup body 3. A drain pipe 15 is fixedly connected to the lower left side of the cooling sleeve 8, and an inlet pipe 14 is fixedly connected to the upper right side of the cooling sleeve 8. The end of the drain pipe 15 away from the cooling sleeve 8 is slidably connected to the inner side of the upper part of the connecting sleeve 17. The threaded sleeve 32 is threadedly connected to the inlet pipe 14.

[0029] When the cup body 3 needs to be cooled, push the cooling sleeve 8 to move the inlet pipe 14 and the outlet pipe 15, so that the outlet pipe 15 slides into the connecting sleeve 17. Then rotate the threaded sleeve 32 to connect the inlet pipe 14 and the delivery pipe 20. Then control the start of the delivery pump 18 to run the suction pipe 19 to draw the coolant into the delivery pump 18. The coolant is then input into the cooling chamber 9 through the delivery pipe 20 and the inlet pipe 14 to cool the cup body 3. Finally, the coolant is introduced into the cooling box 10 through the outlet pipe 15 and the guide pipe 16 for recooling. This achieves efficient cooling of the cup body 3 by the shearing device, effectively offsetting the heat generated by the rotation of the small motor 23 and the shearing of the blade.

[0030] Please see the appendix Figure 1 - Appendix Figure 5The upper part of the cup body 3 is provided with a sealing component for sealing the cup body 3. The sealing component includes an upper cover 4, which is located on the upper outer side of the cup body 3. A sealing gasket 7 is fixedly connected to the bottom of the upper cover 4. A feed tube 5 is fixedly connected to the upper right side of the upper cover 4. A sealing plug 6 is slidably connected inside the feed tube 5. The upper cover 4 is made of stainless steel, and both the sealing plug 6 and the sealing gasket 7 are made of silicone rubber.

[0031] When the chicken embryo tissue needs to be sealed, push the sealing plug 6 to slide it into the feed tube 5, then add the peeled chicken embryo tissue into the cup body 3, and then rotate the top cover 4 to install it on the upper outer side of the cup body 3. The top cover 4 is made of stainless steel to ensure its strength and corrosion resistance. The sealing gasket 7 fills the connection between the cup body 3 and the top cover 4 to ensure airtightness. The sealing plug 6 and the sealing gasket 7 are made of silicone rubber to ensure the elasticity and sealing performance of the sealing plug 6 and the sealing gasket 7. After the chicken embryo tissue is shredded, pull the sealing plug 6 to detach it from the feed tube 5, and directly aspirate the tissue suspension through a large-diameter pipette for subsequent digestion steps. This achieves a highly efficient shredding device that can seal the chicken embryo tissue, prevent external aerosol contamination, and prevent tissue fragments from splashing. The control panel 13 can also be set with cleaning control logic to control the motor to run in high-speed forward and reverse alternating operation after the cleaning solution is added, using fluid turbulence to clean the moving blade 30, the stationary blade 27, and the cup wall in situ. Furthermore, since the magnet 28 and the disk 25 are driven by magnetic coupling, and the cup body 3 and the mounting slot 2 are connected by a non-mechanically locked sliding connection, the cup body 3 and its internal components, including the rotating shaft 29 and the moving blade 30, can be separated from the support frame 1 as a whole, so as to be placed into the high-pressure steam sterilization equipment for sterilization.

[0032] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A closed-type high-efficiency tissue mincing device for primary chicken embryo cell extraction, comprising a support frame (1), characterized in that, The support frame (1) has an installation groove (2) in the upper middle part, and a cup body (3) is slidably connected inside the installation groove (2). A cooling box (10) is fixedly connected to the lower part of the support frame (1). A liquid guide tube (16) is fixedly connected to the upper left side of the cooling box (10). A connecting sleeve (17) is fixedly connected to the upper outer side of the liquid guide tube (16). An infusion pump (18) is fixedly connected to the upper right side of the cooling box (10). A suction tube (19) is fixedly connected to the input end of the infusion pump (18). The output end of the infusion pump (18) is fixedly connected to... There is an infusion tube (20), and a threaded sleeve (32) is threadedly connected to the outer side of the end of the infusion tube (20) away from the infusion pump (18). A control panel (13) is fixedly connected to the upper front side of the support frame (1), and a cooler (12) is fixedly connected to the lower front side of the support frame (1). A shearing component is provided inside the cup body (3) for shearing chicken embryo tissue. A cooling component is provided on the outer side of the cup body (3) for cooling the cup body (3). A sealing component is provided on the upper part of the cup body (3) for sealing the cup body (3).

2. The closed-type high-efficiency tissue mincing device for primary chicken embryo cell extraction according to claim 1, characterized in that, The shearing assembly includes a magnet (28), which is located at the bottom of the cup body (3). A rotating shaft (29) is fixedly connected to the upper part of the magnet (28). Moving blades (30) are evenly fixedly connected to the outer periphery of the rotating shaft (29). A hanging ring (31) is fixedly connected to the upper part of the rotating shaft (29). A stationary blade (27) is evenly fixedly connected to the lower periphery of the inner wall of the cup body (3). A waterproof box (22) is fixedly connected to the bottom of the cooling box (10). A small motor (23) is fixedly connected to the bottom of the waterproof box (22). A turntable (24) is fixedly connected to the output end of the small motor (23). A disk (25) is fixedly connected to the upper part of the turntable (24).

3. The closed-type high-efficiency tissue mincing device for primary chicken embryo cell extraction according to claim 1, characterized in that, The cooling assembly includes a cooling sleeve (8), which is fixedly connected to the outside of the cup body (3). A cooling chamber (9) is provided on the side of the cooling sleeve (8) opposite to the cup body (3). A drain pipe (15) is fixedly connected to the lower left side of the cooling sleeve (8), and an inlet pipe (14) is fixedly connected to the upper right side of the cooling sleeve (8).

4. The closed-type high-efficiency tissue mincing device for primary chicken embryo cell extraction according to claim 1, characterized in that, The sealing assembly includes an upper cover (4), which is located on the upper part of the outer side of the cup body (3). A sealing gasket (7) is fixedly connected to the bottom of the upper cover (4). A feed pipe (5) is fixedly connected to the upper right side of the upper cover (4). A sealing plug (6) is slidably connected inside the feed pipe (5).

5. A closed-type high-efficiency tissue mincing device for primary chicken embryo cell extraction according to claim 1, characterized in that, Limiting blocks (26) are fixedly connected to the lower left and right sides of the outer side of the cup body (3), and limiting grooves (21) are opened on the upper left and right sides of the support frame (1).

6. The closed-type high-efficiency tissue mincing device for primary chicken embryo cell extraction according to claim 1, characterized in that, The cooling tank (10) is fixedly connected to support pads (11) around its lower perimeter, and the end of the liquid extraction pipe (19) away from the infusion pump (18) is fixedly connected to the cooling tank (10).

7. A closed-type high-efficiency tissue mincing device for primary chicken embryo cell extraction according to claim 2, characterized in that, The magnet (28) is connected to the disk (25) by magnetic force, and the moving blade (30) and the stationary blade (27) form an interleaved structure.

8. A closed-type high-efficiency tissue mincing device for primary chicken embryo cell extraction according to claim 3, characterized in that, The end of the drain pipe (15) away from the cooling sleeve (8) is slidably connected to the upper inner side of the connecting sleeve (17), and the threaded sleeve (32) is threadedly connected to the inlet pipe (14).

9. A closed-type high-efficiency tissue mincing device for primary chicken embryo cell extraction according to claim 4, characterized in that, The top cover (4) is made of stainless steel, and the sealing plug (6) and sealing gasket (7) are both made of silicone rubber.

10. A closed-type high-efficiency tissue mincing device for primary chicken embryo cell extraction according to claim 5, characterized in that, The limiting block (26) is slidably connected inside the limiting groove (21).

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