Anti-adhesion dressing for promoting healing of burn wounds and its preparation method
The dressing, composed of a soft microfiber anti-adhesion membrane and a moisturizing layer, solves the problem of dressing adhesion to the wound, promotes the healing of burn wounds, reduces damage during dressing changes, and improves treatment outcomes.
Patent Information
- Application Number
- CN202411933216.3
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-12-26
- Publication Date
- 2026-06-26
AI Technical Summary
Excessive gaps between the dressing and the wound can cause the dressing to adhere to the wound tissue, affecting the wound healing process and increasing patient suffering.
The dressing consists of two layers: a soft microfiber anti-adhesion membrane and a moisturizing layer. The anti-adhesion membrane is prepared by electrospinning technology and cross-linked with propyl gallate solution. Combined with hydrogel or wet gauze as the moisturizing layer, the dressing is adapted to the wound surface.
It effectively prevents dressings from adhering to the wound, provides a suitable moist environment, promotes wound healing, reduces damage during dressing changes, and improves treatment outcomes.
Smart Images
Figure CN122272866A_ABST
Abstract
Description
Technical Field
[0001] This invention relates to the field of burn dressing technology, specifically to an anti-adhesion dressing that promotes burn wound healing and its preparation method. Background Technology
[0002] Burns are an extremely severe form of trauma, significantly impacting a patient's bodily functions. The quality of treatment and care for burn wounds is crucial, directly affecting the patient's recovery process and even survival. Burns not only damage the skin's barrier function but can also lead to a series of problems such as severe infection, fluid loss, and metabolic disorders. During treatment, meticulous care of burn wounds is necessary to prevent the spread of infection and promote wound healing. Good treatment and care can minimize the occurrence of complications and improve the patient's survival rate and quality of life. For example, improper wound management in severe burn patients can lead to life-threatening complications such as sepsis; while effective nursing measures can accelerate wound healing, reduce patient suffering, and shorten hospital stays.
[0003] Dressings are essential instruments in the care of burn wounds. They primarily play a crucial role in isolating and protecting the wound. Dressings form a physical barrier between the wound and the external environment, preventing bacteria, dust, and other foreign objects from entering the wound and reducing the risk of infection. Furthermore, different types of dressings have their own unique functions. Some functional dressings can even actively promote wound healing. For example, dressings containing growth factors can stimulate cell proliferation and differentiation, accelerating the wound repair process; hydrogel dressings can provide a moist environment for the wound, which is conducive to the migration and regeneration of epithelial cells. These functional dressings work synergistically through multiple mechanisms to create favorable conditions for the healing of burn wounds.
[0004] During various stages of wound healing, dressing changes often result in adhesions to the wound surface, significantly hindering recovery and increasing patient discomfort. The primary cause of adhesions is excessively large gaps between the dressing and the wound. When the dressing covers the wound, large gaps allow newly formed granulation tissue or fibrous tissue to easily grow through these gaps and become intertwined with the dressing. These adhesions not only cause secondary damage to the wound during dressing changes, leading to increased bleeding and pain, but also impede the normal healing process. For example, forcibly removing adhered dressings may damage newly formed granulation tissue, prolonging healing time; moreover, frequent adhesions and dressing removal can cause patients to develop fear and resistance to treatment, negatively impacting treatment outcomes. Summary of the Invention
[0005] This invention provides an anti-adhesion dressing that promotes burn wound healing and its preparation method, in order to solve the problem of dressing adhesion to wound tissue caused by excessive gap on the side of the dressing in contact with the wound.
[0006] To achieve the above objectives, the present invention adopts the following technical solution: An anti-adhesion dressing for promoting burn wound healing and its preparation method are disclosed. The anti-adhesion dressing consists of two layers: an upper layer is a moisturizing layer, and a lower layer is an anti-adhesion film. The anti-adhesion film is composed of microfibers, and the moisturizing layer is wet gauze or hydrogel.
[0007] Furthermore, the method for preparing the anti-adhesion film is as follows: S1: Add silk fibroin to hexafluoroisopropanol and stir thoroughly until the silk fibroin is completely dissolved to obtain a silk fibroin solution with a concentration of 10-15%. S2: Place the silk fibroin solution into the syringe and prepare an anti-adhesion membrane using an electrospinning device. Set the voltage to 15-25kV, the injection speed to 0.3-0.8mL / h, the receiving distance to 12-18cm, and the spinning time to 2-6h. S3: The anti-adhesion film obtained by electrospinning is immersed in propyl gallate solution and reacted at 37°C for 24-48 hours. The film is then removed, rinsed repeatedly with deionized water, and finally dried at 30-40°C before being irradiated for sterilization and packaging.
[0008] Furthermore, the upper and lower layers are fixed together by stitching, gluing, or physical pressing.
[0009] Furthermore, the preparation method of the dressing with the upper layer being wet gauze is as follows: soak medical degreased gauze in physiological saline or drug solution until it is completely soaked, take out the gauze, and gently squeeze out the excess drug solution; gently cover the center of the gauze with the anti-adhesion film and fix it.
[0010] Furthermore, the medicinal solution includes: gentamicin solution, growth factor solution, phellodendron bark solution, or comfrey solution.
[0011] Furthermore, the preparation method of the dressing with a hydrogel upper layer is as follows: S11: Dissolve sodium alginate powder in deionized water to form a 3% w / v sodium alginate solution; S12: Slowly add chitosan powder to a 1% v / v glacial acetic acid solution to obtain a 2% w / v chitosan solution; S13: Mix sodium alginate solution and chitosan solution in a 1:1 ratio, and slowly add the mixture dropwise to a 2% w / v calcium chloride solution using a syringe, allowing it to react for 30-60 minutes to form a hydrogel; S14: Soak the hydrogel in deionized water for 2-3 hours and then sterilize it in an autoclave. S15: Apply a 2% w / v calcium chloride solution to the four sides of the anti-adhesion membrane, place the anti-adhesion membrane in the middle of the hydrogel, place small sterile weights around the hydrogel and the anti-adhesion membrane, apply pressure and maintain for 10-20 minutes.
[0012] Furthermore, the water absorption rate of the dressing is 18.3%.
[0013] Furthermore, the cell adhesion rate of the dressing is 10%.
[0014] Furthermore, the tensile strength of the anti-adhesion film obtained by spinning a 15% silk fibroin solution at 20kV, a rate of 0.8mL / h, a receiving distance of 12cm, and for 2h is greater than 10MPa.
[0015] Compared with the prior art, the present invention has the following beneficial effects: This invention employs a two-layer structure consisting of an anti-adhesion film and a moisturizing layer, which can meet both the need for anti-adhesion and the need for wound moisturization during the wound healing stage. The anti-adhesion membrane prepared by this invention is an ultrafine fiber anti-adhesion membrane, which has a good anti-adhesion effect. The microfiber adhesive membrane of the present invention is made of soft silk fibroin electrospun and cross-linked by soaking in propyl gallate solution, which improves the tensile strength of the microfiber adhesive membrane and makes it suitable for wound dressing. This invention provides two types of dressings. One type is used in conjunction with clinically common gauze, which facilitates the clinical promotion and low-cost use of the product. The other type is a dressing with an integrated hydrogel as a moisturizing layer. The hydrogel of this invention, when used as a moisturizing dressing, has good water absorption and low cell adhesion. Attached Figure Description
[0016] Figure 1 This is a photograph of the anti-adhesion membrane for promoting burn wound healing according to Embodiment 1 of the present invention; Figure 2 This is a scanning electron microscope image of the anti-adhesion membrane for promoting burn wound healing in Embodiment 1 of the present invention; Figure 3 This is the cell adhesion experiment result of the anti-adhesion dressing for promoting burn wound healing in Example 4 of the present invention; Figure 4 This is a comparative result of an in vivo animal experiment in mice of the anti-adhesion membrane that promotes burn wound healing, as described in Example 5 of this invention. Detailed Implementation
[0017] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the embodiments described are only some embodiments of the present invention, and not all embodiments.
[0018] One key component of the anti-adhesion dressing provided by this invention is that the part in contact with the wound is an anti-adhesion membrane material. Various anti-adhesion membranes are available on the market for postoperative use, but they are unsuitable for burn wounds because: their mechanical strength is too high for wound application; and their texture is usually too hard and brittle, making them unsuitable for wound application. Therefore, there is a need to develop a soft anti-adhesion membrane with sufficient mechanical strength.
[0019] The wound healing stage here refers to the proliferative phase, which is characterized by the formation of granulation tissue. In addition to maintaining a moist environment to promote tissue growth, the dressings for this stage also need to prevent adhesion and avoid damaging the newly formed tissue when changing the dressing. Example 1
[0020] Anti-adhesion dressings that promote burn wound healing consist of two layers. The upper layer is a moisturizing dressing, such as a hydrogel dressing or soaked gauze; the lower layer is the crucial anti-adhesion membrane, characterized by its composition of ultrafine fibers. Various methods can be used to secure the upper and lower layers together, such as sutures or adhesive bonding. The diameter of the ultrafine fibers is between 2-5 μm.
[0021] The preparation method of the microfiber anti-adhesion membrane in this embodiment is as follows: S1: Add silk fibroin to hexafluoroisopropanol and stir thoroughly until the silk fibroin is completely dissolved to obtain a silk fibroin solution with a concentration of 10-15%. S2: Place the silk fibroin solution into the syringe and prepare an anti-adhesion membrane using an electrospinning device. Set the voltage to 15-25kV, the injection speed to 0.3-0.8mL / h, the receiving distance to 12-18cm, and the spinning time to 2-6h. S3: The anti-adhesion film obtained by electrospinning is immersed in propyl gallate solution and reacted at 37°C for 24-48 hours. The film is then removed, rinsed repeatedly with deionized water, and finally dried at 30-40°C before being irradiated for sterilization and packaging.
[0022] Appendix Figure 1 An anti-adhesion film was obtained by spinning a 15% silk fibroin solution at 20kV for 2 hours at a rate of 0.8mL / h and a receiving distance of 12cm. Figure 2 for Figure 1Scanning electron microscopy (SEM) images of the anti-adhesion membrane show an average fiber diameter of 2.8 μm. The anti-adhesion membrane prepared in this embodiment, because it uses silk fibroin and is cross-linked with propyl gallate, has a soft texture and certain mechanical strength; its tensile strength exceeds 10 MPa. This is significantly different from anti-adhesion membranes used postoperatively (with a mechanical strength of 1-2 MPa and a brittle texture). Example 2
[0023] Based on Example 1, an anti-adhesion dressing that promotes the healing of burn wounds was prepared. In this example, gauze commonly used in clinical practice was processed and used as the top dressing. Its advantage is that it can be assembled using locally available materials.
[0024] Soak medical degreased gauze in physiological saline or medicine solution until fully saturated. Remove the gauze, gently squeeze out excess medicine solution, and gently cover the center of the gauze with the anti-adhesion film prepared in Example 1. Apply the dressing to the wound.
[0025] There are several options for the medication. For example, gentamicin solution can be used to prevent wound infection; or growth factor solution can be used to promote wound cell proliferation and healing; or Phellodendron bark extract can be used, which has the effects of clearing heat and drying dampness, purging fire and detoxifying, and can inhibit wound inflammation; or Lithospermum erythrorhizon extract can be used, which has the effects of cooling blood, promoting blood circulation, and detoxifying, and can promote wound healing and reduce scar formation. Example 3
[0026] Based on Example 1, the upper layer uses a hydrogel dressing, which provides an integrated dressing.
[0027] S11: Dissolve sodium alginate powder in deionized water to form a 3% w / v sodium alginate solution; S12: Slowly add chitosan powder to a 1% v / v glacial acetic acid solution to obtain a 2% w / v chitosan solution; S13: Mix sodium alginate solution and chitosan solution in a 1:1 ratio, and slowly add the mixture dropwise to a 2% w / v calcium chloride solution using a syringe, allowing it to react for 30-60 minutes to form a hydrogel; S14: Soak the hydrogel in deionized water for 2-3 hours and then sterilize it in an autoclave. S15: Apply a 2% w / v calcium chloride solution to the four sides of the anti-adhesion membrane, place the anti-adhesion membrane in the middle of the hydrogel, place small sterile weights around the hydrogel and the anti-adhesion membrane, apply pressure and maintain for 10-20 minutes. Example 4
[0028] The dressing of Example 3 was subjected to dressing performance tests. This example describes the process and results of the dressing absorbency test, protein adsorption test, and cell adhesion test.
[0029] Dressing absorbency test experiment: Weigh the dressing W d Soak the dressing in phosphate buffer solution at 25°C for 6 hours to ensure it is fully swollen. Remove the dressing, blot off the surface water with filter paper, and weigh it (W). w Each sample was tested in parallel 5 times, and the water absorption performance of the dressing was calculated. The average water absorption performance was 18.3%.
[0030] Cell adhesion experiment: Cut the dressing and lay it evenly on the bottom of the culture plate. Sterilize by soaking in 75% ethanol for 2 hours, then rinse repeatedly with phosphate buffer solution to remove the ethanol. Inoculate the dressing surface with a seed quantity of 4 × 10⁶. 4 Human fibroblasts were collected and 2 ml of culture medium was added. After incubation at 37°C for 4 h, the cells were removed and rinsed with phosphate buffer to remove non-viscous cells. 20 μL of 0.5 wt% MTT phosphate buffer was added and incubated at 37°C for 4 h. 200 μL of dimethyl sulfoxide was added and the cells were shaken well. The absorbance was measured at 570 nm using an ELISA reader.
[0031] In the experiment, the control group used gauze, while the experimental group used the dressing from Example 3. The experimental results are as follows: Figure 3 As shown in the results, the cell adhesion rate of the control group was 0.23, while that of the experimental group was 0.10, indicating a significant reduction in cell adhesion rate, which effectively prevents adhesion. Example 5
[0032] This embodiment is an intraperitoneal animal experiment conducted on the anti-adhesion membrane of Example 1, used to verify the efficacy of the anti-adhesion membrane alone. The control group was a blank group, and the commercial control group was the commercially available anti-adhesion membrane (INTERCEED). The experimental results are as follows: Figure 4 As shown in the results, the anti-adhesion effect of the anti-adhesion membrane in Example 1 is comparable to that of commercially available products specifically designed for intra-abdominal anti-adhesion, which is sufficient to meet the anti-adhesion requirements of burn wounds.
[0033] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, and not to limit them; although the present invention has been described in detail with reference to the above embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or make equivalent substitutions for some or all of the technical features, and these modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the technical solutions of the embodiments of the present invention.
Claims
1. A non-adhesive dressing for promoting burn wound healing and its preparation method, characterized in that, The anti-adhesion dressing consists of two layers: an upper layer that is moisturizing and a lower layer that is an anti-adhesion film. The anti-adhesion film is made of microfiber, and the moisturizing layer is wet gauze or hydrogel.
2. The anti-adhesion dressing for promoting burn wound healing and its preparation method according to claim 1, characterized in that, The method for preparing the anti-adhesion film is as follows: S1: Add silk fibroin to hexafluoroisopropanol and stir thoroughly until the silk fibroin is completely dissolved to obtain a silk fibroin solution with a concentration of 10-15%. S2: Place the silk fibroin solution into the syringe and prepare an anti-adhesion membrane using an electrospinning device. Set the voltage to 15-25kV, the injection speed to 0.3-0.8mL / h, the receiving distance to 12-18cm, and the spinning time to 2-6h. S3: The anti-adhesion film obtained by electrospinning is immersed in propyl gallate solution and reacted at 37°C for 24-48 hours. The film is then removed, rinsed repeatedly with deionized water, and finally dried at 30-40°C before being irradiated for sterilization and packaging.
3. The anti-adhesion dressing for promoting burn wound healing and its preparation method according to claim 1, characterized in that, The upper and lower layers are fixed together by stitching, gluing, or physical pressing.
4. The anti-adhesion dressing for promoting burn wound healing and its preparation method according to claim 1, characterized in that, The preparation method of the dressing with the upper layer being wet gauze is as follows: soak medical degreased gauze in physiological saline or drug solution until it is completely soaked, take out the gauze, and gently squeeze out the excess drug solution; gently cover the center of the gauze with the anti-adhesion film and fix it.
5. The anti-adhesion dressing for promoting burn wound healing according to claim 4, and its preparation method, characterized in that, The medicinal solution includes: gentamicin solution, growth factor solution, Phellodendron bark solution, or Lithospermum erythrorhizon solution.
6. The anti-adhesion dressing for promoting burn wound healing and its preparation method according to claim 1, characterized in that, The preparation method for a dressing with a hydrogel upper layer is as follows: S11: Dissolve sodium alginate powder in deionized water to form a 3% w / v sodium alginate solution; S12: Slowly add chitosan powder to a 1% v / v glacial acetic acid solution to obtain a 2% w / v chitosan solution; S13: Mix sodium alginate solution and chitosan solution in a 1:1 ratio, and slowly add the mixture dropwise to a 2% w / v calcium chloride solution using a syringe, allowing it to react for 30-60 minutes to form a hydrogel; S14: Soak the hydrogel in deionized water for 2-3 hours and then sterilize it in an autoclave. S15: Apply a 2% w / v calcium chloride solution to the four sides of the anti-adhesion membrane, place the anti-adhesion membrane in the middle of the hydrogel, place small sterile weights around the hydrogel and the anti-adhesion membrane, apply pressure and maintain for 10-20 minutes.
7. The anti-adhesion dressing for promoting burn wound healing according to claim 6, and its preparation method, characterized in that, The absorbency of the dressing is 18.3%.
8. The anti-adhesion dressing for promoting burn wound healing according to claim 6, and its preparation method, characterized in that, The cell adhesion rate of the dressing is 10%.
9. The anti-adhesion dressing for promoting burn wound healing according to claim 2, and its preparation method, characterized in that, A 15% silk fibroin solution, spun at 20kV, with a rate of 0.8mL / h and a receiving distance of 12cm for 2h, yielded an anti-adhesion film with a tensile strength greater than 10MPa.