Penicillium oxalicum and trichoderma asperellum organic fertilizer and preparation method thereof

Abstract

This invention relates to the field of fertilizers, specifically to an organic fertilizer containing *Penicillium oxalate* and *Trichoderma echinococcosis*, and its preparation method. The organic fertilizer, by weight percentage, comprises: 10%-25% *Penicillium oxalate*, 10%-25% *Trichoderma echinococcosis*, 10%-20% sheep manure, 5%-10% ammonium phosphate, 5%-10% potassium fertilizer, 10%-20% straw ash, 1%-5% glyphosate, 1%-5% 6-benzylaminopurine, with a binder to make up 100%. The active ingredients of this invention are *Penicillium oxalate* and *Trichoderma echinococcosis*. It is mainly used to increase the yield of vegetables, flowers, and other crops while preventing fungal diseases such as anthracnose and black rot.

Description

[0001] manual Technical Field

[0002] This invention relates to the field of fertilizer technology, and in particular to an organic fertilizer containing Penicillium oxalate and Trichoderma hygroscopicum and its preparation method. Background Technology

[0003] China is a major agricultural country, and agricultural production is the foundation of its economy, consuming large quantities of chemical fertilizers annually. The irrational use of chemical fertilizers and the large amounts of waste generated from industrial and agricultural production have caused serious environmental pollution. Therefore, promoting green and organic agricultural products, improving soil fertility and quality in farmland, and researching, developing, and using organic fertilizers through multiple channels are of significant practical importance for reducing environmental pollution, protecting the environment, comprehensively utilizing natural organic matter, and ensuring food safety.

[0004] The mechanisms by which *Penicillium oxalate* is used for plant disease control include inducing resistance and secreting enzymatic substances. Through metabolism, it produces antifungal substances that inhibit the growth of various pathogenic fungi, such as antagonizing *Sclerotinia sclerotinia* and inhibiting its mycelial growth. Recent research reports that *Penicillium oxalate* also has phosphorus-soluble and pesticide-degrading functions, while simultaneously promoting growth and improving the quality of agricultural products.

[0005] Trichoderma is widely found in soil, plant rhizosphere, leaves, bark, and fallen leaves. It has a strong ability to grow and reproduce, is highly adaptable to the environment, and can easily establish a dominant population. It can also produce a variety of antagonistic metabolites, abundant enzymes, and growth-promoting factors, making it one of the most widely used probiotics.

[0006] Currently, domestic research on Trichoderma mainly focuses on Trichoderma harzianum, Trichoderma viride, Trichoderma variegata, Trichoderma reesei, and Trichoderma longifolia. Trichoderma echinosporum, however, is a newly recorded Trichoderma species in my country in recent years, and its development and utilization are still in their initial stages.

[0007] The *Trichoderma echinosporum* fungus used in this invention can promote the growth of plant seedlings and control diseases such as anthracnose and black rot in crops. It is environmentally friendly, safe, harmless, and pollution-free. It not only controls fruit and vegetable diseases but also promotes seedling growth, increasing plant height, root length, root fresh weight, stem and leaf fresh weight, and total fresh weight, thus improving yield. Summary of the Invention

[0008] In view of this, the present invention provides an organic fertilizer that can rapidly improve soil, enhance soil water and fertilizer retention capacity, and maintain a dynamic balance between soil microbial reproduction and plant growth. Simultaneously, it improves the living standards and health of urban and rural residents, reduces the burden on farmers, increases their income, and promotes social and economic benefits. This organic fertilizer can improve soil, increase crop yield, and provide a certain degree of disease resistance to crops. The preparation process is simple, the production cost is low, and the fertilizer efficiency is high, resulting in good effects.

[0009] To achieve the above objectives, the present invention adopts the following technical solution:

[0010] This invention provides an organic fertilizer containing Penicillium oxalate and Trichoderma echinosporum, wherein the organic fertilizer contains Penicillium oxalate and Trichoderma echinosporum by weight percentage: 10%-25% Penicillium oxalate, 10%-25% Trichoderma echinosporum, 10%-20% sheep manure, 5%-10% ammonium phosphate, 5%-10% potassium fertilizer, 10%-20% straw ash, 1%-5% glyphosate, 1%-5% 6-benzylaminopurine, and binder to make up 100%.

[0011] Another aspect of the present invention provides a method for preparing organic fertilizer containing Penicillium oxalate and Trichoderma hygroscopicum, comprising the following steps:

[0012] (1) Add Penicillium oxalate, Trichoderma echinosporum, sheep manure, ammonium phosphate, potassium fertilizer, straw ash, glyphosate, 6-benzylaminopurine, and binder to the mixture. Crush each ingredient separately.

[0013] (2) Weigh each product obtained in step (1) according to the weight ratio, mix them thoroughly and add them to a granulator for granulation. The particle size of the granulated particles is 3-5 mm. Dry them until the moisture content is below 15% to obtain the finished organic fertilizer containing Penicillium oxalate and Trichoderma hygroscopica.

[0014] (3) A further technical solution is: in step (1), Penicillium oxalate, Trichoderma acicularis, sheep manure, ammonium phosphate, potassium fertilizer, straw ash, glyphosate, 6-benzylaminopurine, and binder are crushed to 45 mesh.

[0015] The beneficial effects of this invention are as follows: The organic fertilizer containing Penicillium oxalate and Trichoderma echinosporum provided by this invention has the advantages of wide availability and comprehensive nutrients, and can improve soil, enhance soil fertility, increase yield, and improve quality. Applying more of this organic fertilizer can improve soil activity and biological reproduction and transformation capacity, thereby improving the soil's absorption capacity, buffering capacity, and stress resistance. Detailed Implementation

[0016] To make the objectives, technical solutions, and advantages of the present invention clearer, the embodiments of the present invention will be described in detail below.

[0017] Example 1

[0018] A method for preparing organic fertilizer containing Penicillium oxalate and Trichoderma echinosporum, characterized in that the formula contains the following raw materials in weight percentage: 10% Penicillium oxalate, 10% Trichoderma echinosporum, 20% sheep manure, 5% ammonium phosphate, 5% potassium fertilizer, 10% straw ash, 1% glyphosate, 1% 6-benzylaminopurine, and bentonite to make up 100%.

[0019] The specific steps of the proposed method for preparing organic fertilizer containing Penicillium oxalate and Trichoderma echinosporum are as follows:

[0020] 1) Crushing: Place Penicillium oxalate, Trichoderma hygroscopicum, sheep manure, ammonium phosphate, potassium fertilizer, straw ash, glyphosate, 6-benzylaminopurine, and bentonite into a crusher and crush them to 45 mesh.

[0021] 2) Granulation: Weigh the products obtained in 1) according to the weight ratio, mix them thoroughly, and then add them to an extrusion granulator for granulation. The resulting particles have a radius of 3-5 mm.

[0022] 3) Drying: Place the granular product obtained in 2) into a dryer for drying. The finished organic fertilizer containing Penicillium oxalate and Trichoderma hygroscopicum can be obtained when the moisture content is less than 15%.

[0023] Example 2

[0024] A method for preparing organic fertilizer containing Penicillium oxalate and Trichoderma echinosporum, characterized in that the formula contains the following raw materials in weight percentage: 15% Penicillium oxalate, 15% Trichoderma echinosporum, 15% sheep manure, 6% ammonium phosphate, 7% potassium fertilizer, 15% straw ash, 1% glyphosate, 2% 6-benzylaminopurine, and bentonite to make up 100%.

[0025] The specific steps of the proposed method for preparing organic fertilizer containing Penicillium oxalate and Trichoderma echinosporum are as follows:

[0026] 1) Crushing: Place Penicillium oxalate, Trichoderma hygroscopicum, sheep manure, ammonium phosphate, potassium fertilizer, straw ash, glyphosate, 6-benzylaminopurine, and bentonite into a crusher and crush them to 45 mesh.

[0027] 2) Granulation: Weigh the products obtained in 1) according to the weight ratio, mix them thoroughly, and then add them to an extrusion granulator for granulation. The resulting particles have a radius of 3-5 mm.

[0028] 3) Drying: Place the granular product obtained in 2) into a dryer for drying. The finished organic fertilizer containing Penicillium oxalate and Trichoderma hygroscopicum can be obtained when the moisture content is less than 15%.

[0029] Example 3

[0030] A method for preparing organic fertilizer containing Penicillium oxalate and Trichoderma echinosporum, characterized in that the formula contains the following raw materials in weight percentage: 20% Penicillium oxalate, 20% Trichoderma echinosporum, 10% sheep manure, 5% ammonium phosphate, 8% potassium fertilizer, 10% straw ash, 2% glyphosate, 3% 6-benzylaminopurine, and bentonite to make up 100%.

[0031] 1) Crushing: Place Penicillium oxalate, Trichoderma hygroscopicum, sheep manure, ammonium phosphate, potassium fertilizer, straw ash, glyphosate, 6-benzylaminopurine, and bentonite into a crusher and crush them to 45 mesh.

[0032] 2) Granulation: Weigh the products obtained in 1) according to the weight ratio, mix them thoroughly, and then add them to an extrusion granulator for granulation. The resulting particles have a radius of 3-5 mm.

[0033] 3) Drying: Place the granular product obtained in 2) into a dryer for drying. The finished organic fertilizer containing Penicillium oxalate and Trichoderma hygroscopicum can be obtained when the moisture content is less than 15%.

[0034] Example 4

[0035] A method for preparing organic fertilizer containing Penicillium oxalate and Trichoderma echinosporum, characterized in that the formula contains the following raw materials in weight percentage: 25% Penicillium oxalate, 25% Trichoderma echinosporum, 10% sheep manure, 10% ammonium phosphate, 8% potassium fertilizer, 10% straw ash, 2% glyphosate, 3% 6-benzylaminopurine, and bentonite to make up 100%.

[0036] 1) Crushing: Place Penicillium oxalate, Trichoderma hygroscopicum, sheep manure, ammonium phosphate, potassium fertilizer, straw ash, glyphosate, 6-benzylaminopurine, and bentonite into a crusher and crush them to 45 mesh.

[0037] 2) Granulation: Weigh the products obtained in 1) according to the weight ratio, mix them thoroughly, and then add them to an extrusion granulator for granulation. The resulting particles have a radius of 3-5 mm.

[0038] 3) Drying: Place the granular product obtained in 2) into a dryer for drying. The finished organic fertilizer containing Penicillium oxalate and Trichoderma hygroscopicum can be obtained when the moisture content is less than 15%.

[0039] To further investigate the effects of organic fertilizer containing Penicillium oxalate and Trichoderma echinosporum, its influence on the growth and disease resistance of fruit (strawberries) will be verified.

[0040] The following is a further evaluation of the effects of the organic fertilizer containing Penicillium oxalate and Trichoderma echinococcosis prepared in the above examples on the growth and disease resistance of fruit (strawberries).

[0041] Equal amounts of organic fertilizers containing Penicillium oxalate and Trichoderma hygroscopicum prepared in Examples 1-4 were applied to the cultivation of small strawberries. A control group was set up with no fertilizer applied. Organic fertilizer made from traditional animal excrement was used as Comparative Example 1, and organic fertilizer made from traditional crop straw was used as Comparative Example 2 (the amount of fertilizer applied to the comparative examples was the same as that in Examples 1-4). All experimental groups used strawberry seedlings with similar growth and were cultivated under the same conditions. After 30 days of cultivation, the survival rate, plant height, and disease incidence of the strawberries were detected and statistically analyzed.

[0042] Table 1

[0043] Group Comparative Example 1 Comparative Example 2 Example 1 Example 2 Example 3 Example 4 Survival rate (%) 80.5 83.5 90.1 92.5 95.6 97.5 Disease incidence rate (%) 21.3 25.3 9.1 8.3 6.5 5.2 Plant height (cm) 36.7 37.5 49.6 53.7 53.9 55.4

[0044] Table 1. Effects of the organic fertilizer containing Penicillium oxalate and Trichoderma hydatopsus of this invention on the growth of fruit (strawberry).

[0045] As can be seen from Table 1, the organic fertilizer containing Penicillium oxalate and Trichoderma hygroscopica prepared in this invention can significantly improve the survival rate of strawberries, promote strawberry growth, and reduce the incidence of strawberry diseases.

[0046] The above description is merely a specific embodiment of the present invention, but the scope of protection of the present invention is not limited thereto. Any variations or substitutions that can be easily conceived by those skilled in the art within the technical scope disclosed in the present invention should be included within the scope of protection of the present invention. Therefore, the scope of protection of the present invention should be determined by the scope of the claims.

Claims

1. An organic fertilizer containing Penicillium oxalate and Trichoderma hygroscopicum, and its preparation method, characterized in that, The formula, by weight percentage, is as follows: 10%-25% Penicillium oxalate, 10%-25% Trichoderma echinosporum, 10%-20% sheep manure, 5%-10% ammonium phosphate, 5%-10% potassium fertilizer, 10%-20% straw ash, 1%-5% glyphosate, 1%-5% 6-benzylaminopurine, and binder to make up to 100%.

2. The organic fertilizer containing Penicillium oxalate and Trichoderma hygroscopicum according to claim 1, and its preparation method, comprises the following steps: (1) The following ingredients were pulverized: Penicillium oxalate, Trichoderma echinosporum, sheep manure, ammonium phosphate, potassium fertilizer, straw ash, glyphosate, 6-benzylaminopurine, and binder. The resulting products were weighed according to their respective weight percentages, thoroughly mixed, and then granulated in a granulator to obtain organic fertilizer.

3. The organic fertilizer containing Penicillium oxalate and Trichoderma hygroscopicum according to claim 1, and its preparation method, characterized in that: The 6-benzylaminopurine mentioned above is a synthetically produced cytokinin.

4. The organic fertilizer containing Penicillium oxalate and Trichoderma hygroscopicum according to claim 2, and its preparation method, characterized in that: In step (1), *Penicillium oxalate*, *Trichoderma echinosporum*, sheep manure, ammonium phosphate, potassium fertilizer, straw ash, glyphosate, 6-benzylaminopurine, and binder are pulverized to 45 mesh and thoroughly mixed. The mixture is then placed in a granulator for granulation, resulting in particles with a radius of 3-5 mm. The particles are dried until the moisture content is below 15% to obtain the finished organic fertilizer containing *Penicillium oxalate* and *Trichoderma echinosporum*.

5. The method according to claim 1, characterized in that, The binder is phosphate rock powder, bentonite, diatomaceous earth, kaolin or attapulgite, preferably bentonite.