A naturally shaped nutrient lick brick for musk deer and its preparation method

Abstract

This invention discloses a naturally formed nutritional licking brick specifically for musk deer and its preparation method. Using calcium silicate as the skeleton material, it is compounded with yeast culture powder, bentonite, compound probiotics, sweeteners, and flavoring agents. The resulting licking brick continuously releases soluble nutrients and active bacteria during the musk deer's licking process. The specific combination of probiotics and yeast culture powder components effectively improves the intestinal immunity of captive musk deer, significantly reduces intestinal inflammation, and simultaneously lowers cortisol levels in feces, alleviating stress responses. Using yeast culture powder, calcium silicate, and bentonite as a composite coagulant, the licking brick prepared by mold casting exhibits optimal moisture resistance during storage. Furthermore, the licking brick has a smooth appearance, a compact and sturdy structure, excellent molding performance, and retains its structural integrity even after immersion in water, making it suitable for preparing products related to improving the intestinal health and immunity of musk deer.

Description

Technical Field

[0001] This invention relates to the field of livestock feed technology, specifically to a naturally formed nutritional lick brick for musk deer and its preparation method. Background Technology

[0002] Existing technologies often use antibiotics to enhance the immunity of musk deer and address stress-related issues. However, prolonged antibiotic use can lead to drug resistance and reduce the drug's anti-stress effects. To avoid this resistance, green alternatives to antibiotics need to be developed. Currently, only lick blocks for cattle and sheep are available on the market, not for musk deer. Cattle and sheep lick blocks are formed under high pressure using a hydraulic press, resulting in a solid texture suitable for the larger, rougher tongues of cattle and sheep, but unsuitable for musk deer.

[0003] Therefore, providing a composite lick brick that combines nutrition, bioavailability, durability, and palatability, and is suitable for musk deer, has significant ecological and economic value. Summary of the Invention

[0004] The purpose of this invention is to overcome the shortcomings of the prior art and provide a naturally formed nutritional licking brick for musk deer and its preparation method. Using calcium silicate as the skeleton material, it is compounded with yeast culture powder, bentonite, probiotics, sweeteners and flavorings. Without the need for large hydraulic equipment, the naturally formed licking brick has good mechanical strength and moisture resistance, and can continuously release soluble nutrients and active bacteria during licking, thereby improving the intestinal health and immunity of musk deer, improving the health level and feeding efficiency of musk deer. Its preparation method is simpler and the cost is lower.

[0005] To achieve the above technical effects, the following technical solution is adopted: A naturally shaped nutritional licking brick specifically for musk deer, comprising the following components: 20-35% yeast culture powder, 8-15% silicate, 15-30% bentonite, 12-25% wheat bran, 15-30% corn flour, 1-8% salt, 0.5-2% brewer's yeast, 0.5-2% Bacillus subtilis, 0.5-2% Lactobacillus plantarum, 0.02-0.1% sweetener, and 0.03%-0.2% pineapple milk flavoring agent; Furthermore, the above-mentioned components are added in the following amounts: yeast culture powder 25-30%, calcium silicate 10-15%, bentonite 20-25%, wheat bran 15-20%, corn flour 15-20%, salt 2-5%, brewer's yeast 1-2%, Bacillus subtilis 1-2%, Lactobacillus plantarum 1-2%, sweetener 0.02-0.06%, and pineapple milk flavoring 0.05%-0.15%; Furthermore, the silicate is food-grade calcium silicate; Furthermore, the silicates, bentonite, sweeteners, and pineapple milk flavoring are all feed-grade raw materials; Furthermore, the sweetener is one or more of sodium saccharin, thomaline, steviol glycosides, etc. This invention also provides a method for preparing the above-mentioned naturally formed nutrient licking brick for musk deer, comprising the following steps: Step S1: Weigh and mix calcium silicate, yeast culture powder, wheat bran, corn flour and bentonite, and stir evenly to obtain the first mixture; Step S2: Weigh and mix the salt, brewer's yeast, Bacillus subtilis, Lactobacillus plantarum, sweetener, and pineapple milk flavoring, and stir well to obtain the second mixture; Step S3: Mix the first mixture and the second mixture thoroughly to obtain the third mixture; Step S4: Add water to the third mixture and stir thoroughly until well combined; Step S5: Place the mixture into a mold, and demold it after it solidifies and sets. Furthermore, in step S4, the amount of water added is 20% to 25% of the total weight of the third mixture; Furthermore, after placing the mixture into a cube mold, press the mold to shape the licking brick; Furthermore, after being placed in a cool, ventilated place for 3-10 days, the licking brick naturally solidifies and takes shape. The brick is a cube, with a length / width of 5-7 cm, a height of 2-4 cm, and a density of 1-1.5 g / cm³. 3 ; This invention also provides the application of a naturally shaped nutritional licking brick specifically for musk deer in the preparation of products that improve the intestinal health and immunity of musk deer.

[0006] The beneficial effects of this invention are as follows: This invention uses calcium silicate as the skeleton material, combined with yeast culture powder, bentonite, probiotics, sweeteners, and flavorings to produce a licking brick that possesses good mechanical strength and moisture resistance, and continuously releases soluble nutrients and active bacteria during licking. The specific combination of probiotics and yeast culture powder effectively improves the intestinal immunity of captive musk deer, significantly reduces intestinal inflammation, and significantly reduces cortisol levels in feces, alleviating stress and thus improving health and feeding efficiency. The nutritional licking brick contains three microbial components (Saccharomyces cerevisiae, Bacillus subtilis, and Lactobacillus plantarum) and yeast culture... The dry powder components have a synergistic effect in improving the intestinal immunity of captive musk deer, reducing intestinal inflammation, reducing cortisol content in feces, and protecting the intestinal health of captive musk deer. The lick bricks prepared by mold casting using yeast culture dry powder, calcium silicate, and bentonite as composite coagulants have the best moisture resistance during storage. The lick bricks have a smooth appearance, a compact and sturdy structure, and remain structurally intact after soaking in water and drying, making them usable again. Compared with the pressing method, which requires large machines and high pressure, this method is simpler, cheaper, and has better formability, making it suitable for preparing products related to improving the intestinal health and immunity of musk deer. Attached Figure Description

[0007] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. The drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0008] Figure 1 This is a flowchart illustrating the preparation process of the naturally formed musk deer-specific nutrient licking brick described in this embodiment of the invention. Figure 2 These are molding effect diagrams of the licking bricks prepared in Examples 1-2 and Comparative Examples 1-5 of the present invention, wherein... Figure 2 (a) shows the molding effect of the licking brick prepared in Example 1. Figure 2 (b) shows the molding effect of the licking brick prepared in Example 2, 2(c) shows the molding effect of the licking brick prepared in Comparative Example 1, 2(d) shows the molding effect of the licking brick prepared in Comparative Example 2, 2(e) shows the molding effect of the licking brick prepared in Comparative Example 3, 2(f) shows the molding effect of the licking brick prepared in Comparative Example 4, and 2(g) shows the molding effect of the licking brick prepared in Comparative Example 5. Figure 3 Images of the licking brick prepared in Example 1 of this invention before and after immersion in water; wherein, Figure 3 (a) shows a picture of the brick before it was soaked in water. Figure 3(b) shows a picture of a licking brick soaked in water, 3(c) shows a picture of a licking brick after it has been soaked in water, and 3(d) shows a picture of a licking brick after it has been soaked in water and then dried. Figure 4 Images of the licking brick prepared in Comparative Example 5 of this invention before and after immersion in water; wherein, Figure 4 (a) shows a picture of the brick before it was soaked in water. Figure 4 (b) shows a picture of the licking brick soaked in water, 4(c) shows a picture of the licking brick after soaking in water, and 4(d) shows a picture of the licking brick after soaking in water and drying. Detailed Implementation

[0009] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0010] It should be noted that the following detailed descriptions are exemplary and intended to provide further illustration of the invention. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention pertains.

[0011] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments of the present invention. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, and / or combinations thereof.

[0012] In the following examples, unless otherwise specified, all reagents and consumables were purchased from conventional reagent manufacturers in the art; unless otherwise specified, the experimental methods and techniques used are conventional methods and techniques in the art.

[0013] The following examples illustrate the multifunctional flame retardant and its preparation method provided by the present invention. The scope of protection of the present invention is not limited by the following examples.

[0014] In the following examples and comparative examples, the yeast culture powder was purchased from Angel Yeast Co., Ltd., product name Banglirun; the brewing yeast was purchased from Angel Yeast Co., Ltd., product name Newvia Type I Ruminant Specific; Bacillus subtilis was purchased from Tibet Angel Everest Biotechnology Co., Ltd., product name Bacillus subtilis Type I; and Lactobacillus plantarum was purchased from Tibet Angel Everest Biotechnology Co., Ltd., product name Lactobacillus plantarum LP-S2.

[0015] The calcium silicate is food grade with a purity of ≥99% and was purchased from Hebei Kelongduo Biotechnology Co., Ltd.

[0016] The bentonite was purchased from Anji Runying New Materials Co., Ltd. as feed-grade RY-SL bentonite, the sweetener was purchased from Chengdu Dadi Hank Biotechnology Co., Ltd. as sodium saccharin, and the pineapple milk flavoring was purchased from Chengdu Dadi Hank Biotechnology Co., Ltd., under the trade name Pineapple Milk.

[0017] Example 1 The naturally shaped musk deer-specific nutritional lick brick comprises the following components: based on a total of 100 parts, 25 parts of yeast culture dry powder, 12 parts of food-grade calcium silicate, 20 parts of feed-grade bentonite, 18 parts of wheat bran, 18 parts of corn flour, 3 parts of salt, 1.35 parts of brewing yeast, 1.25 parts of Bacillus subtilis, 1.25 parts of Lactobacillus plantarum, 0.05 parts of feed-grade sweetener, and 0.1 parts of feed-grade pineapple milk flavoring agent.

[0018] See Figure 1 The preparation method of the naturally formed musk deer-specific nutrient lick brick is as follows: Step S1: Take wheat bran and corn and grind them to a particle size of less than 0.4 mm. Weigh each raw material according to the above-mentioned component addition amount. Weigh and mix the food-grade calcium silicate, yeast culture powder, wheat bran, corn flour and feed-grade bentonite, and stir evenly to obtain the first mixture. Step 2: Weigh and mix the salt, brewer's yeast, Bacillus subtilis, Lactobacillus plantarum, feed-grade sweetener, and feed-grade pineapple milk flavoring agent, and stir evenly to obtain the second mixture; Step 3: Mix the first and second mixtures thoroughly to obtain the third mixture; Step 4: Add 25% of the weight of ultrapure water to the third mixture and stir thoroughly until homogeneous; Step 5: Place the mixture into a cube mold, press the mold with your hand to shape the licking brick, and let it stand in a cool and ventilated place for 7 days to naturally solidify and then demold; the finished licking brick is square in shape, with a length / width of 6 cm and a height of 3 cm.

[0019] Example 2 The naturally shaped musk deer-specific nutritional lick brick comprises the following components: 28 parts of yeast culture powder, 10 parts of food-grade calcium silicate, 23 parts of feed-grade bentonite, 16 parts of wheat bran, 16 parts of corn flour, 2 parts of salt, 1.8 parts of brewer's yeast, 1.5 parts of Bacillus subtilis, 1.5 parts of Lactobacillus plantarum, 0.06 parts of feed-grade sweetener, and 0.14 parts of feed-grade pineapple milk flavoring agent. The preparation method of the naturally shaped musk deer-specific nutritional lick brick is the same as in Example 1. The finished lick brick is square in shape, with a length / width of 6 cm and a height of 3 cm.

[0020] Comparative Example 1 The preparation components of the naturally shaped musk deer-specific nutrient lick brick lack yeast culture powder components. At the same time, the amount of food-grade calcium silicate added is 22 parts, the amount of feed-grade bentonite added is 35 parts, and the other components and amounts, as well as the preparation method (except for not adding yeast culture powder), are the same as in Example 1.

[0021] Comparative Example 2 The preparation components of the naturally formed musk deer-specific nutrient lick brick lack feed-grade bentonite, while the amount of food-grade calcium silicate added is 22 parts, the amount of yeast culture dry powder added is 35 parts, and the other components and amounts, as well as the preparation method (except for not adding feed-grade bentonite) are the same as in Example 1.

[0022] Comparative Example 3 The preparation components of the naturally formed musk deer-specific nutrient lick brick lack feed-grade calcium silicate. At the same time, the amount of feed-grade bentonite added is 26 parts, the amount of yeast culture dry powder added is 31 parts, and the other components, amounts, and preparation methods (except for not adding food-grade calcium silicate) are the same as in Example 1.

[0023] Comparative Example 4 Agar was used instead of calcium silicate and bentonite components. The amount of agar added was 32 parts (without adding food-grade calcium silicate and feed-grade bentonite components). Other components, amounts, and preparation methods were the same as in Example 1.

[0024] Comparative Example 5 Feed-grade calcium oxide was used to replace calcium silicate and bentonite components. The amount of calcium oxide added was 32 parts (without adding calcium silicate and bentonite components). Other components, amounts, and preparation methods were the same as in Example 1.

[0025] Comparative Example 6 The preparation components of the optimal naturally shaped musk deer-specific nutrient lick brick lack the brewer's yeast component. At the same time, the amount of Bacillus subtilis is 2 parts, the amount of Lactobacillus plantarum is 1.85 parts, and the other components, amounts, and preparation methods (except for not adding the brewer's yeast component) are the same as in Example 1.

[0026] Comparative Example 7 The preparation components of the optimal naturally shaped musk deer-specific nutrient lick brick lack Bacillus subtilis, while the amount of Saccharomyces cerevisiae is 2 parts, the amount of Lactobacillus plantarum is 1.85 parts, and the other components, amounts, and preparation methods (except for not adding Bacillus subtilis) are the same as in Example 1.

[0027] Comparative Example 8 The preparation components of the optimal naturally shaped musk deer-specific nutrient lick brick lack Lactobacillus plantarum, while the amount of Saccharomyces cerevisiae is 2 parts, the amount of Bacillus subtilis is 1.85 parts, and the other components, amounts, and preparation methods (except for not adding Lactobacillus plantarum) are the same as in Example 1.

[0028] Comparative Example 9 The preparation components of the optimal naturally shaped musk deer-specific nutrient lick brick lack yeast culture dry powder components. At the same time, the amount of brewer's yeast added is 10.35 parts, the amount of Bacillus subtilis added is 9.25 parts, the amount of Lactobacillus plantarum added is 9.25 parts, and the other components, the amount added, and the preparation method are the same as in Example 1.

[0029] Test case The licking bricks prepared in Examples 1-2 and Comparative Examples 1-5 were used to investigate the effects of different coagulants on the molding performance, moisture resistance, and appearance of the licking bricks after immersion.

[0030] Detection indicators and methods 1. Test method for the moisture-proof performance of brick licking: After the pressed licking bricks were cured, they were air-dried at room temperature for 7 days, then crushed. Samples were taken and placed in filter paper packages, sealed, weighed, and recorded. The sealed filter paper packages were placed in a drying oven and dried at 103±2 ℃ for 6±0.1 h. After that, the filter paper packages were removed and placed in a desiccator for 30 min, weighed, and recorded. The formula for calculating moisture content is: Moisture (%) = (Weight before drying - Weight after drying) / Weight before drying × 100%.

[0031] 2. Appearance test conditions for licking bricks after soaking in water: After the pressed bricks have cured, they are air-dried at room temperature for 7 days, then completely immersed in cold water for 24 hours, and then air-dried at room temperature for 7 days.

[0032] Test results 1. Tests on the effects of different coagulants on the molding performance, moisture resistance, and appearance of lick bricks. After demolding, the molding performance of the paving bricks was evaluated. (See [reference needed]). Figure 2 and Table 1, Figure 2 Table 1 shows the molding effect of the licking bricks prepared in Examples 1-2 and Comparative Examples 1-5 of this invention. Table 1 shows the effect of different coagulants on the molding effect of the licking bricks in Examples 1-2 and Comparative Examples 1-5. Among them, Examples 1 and 2 and Comparative Example 5 can be molded; Comparative Example 1 can be molded, but the surface is soft; Comparative Examples 2-4 cannot be molded. Specifically, Comparative Example 2 crumbles upon demolding, and Comparative Example 4 is syrupy and cannot be molded.

[0033] Table 1. Effect of different coagulants on the forming effect of licking bricks

[0034] Moisture resistance is a crucial performance characteristic for the proper use of licking bricks. The different coagulant components in Comparative Examples 1-5 and Examples 1-2 affect the moisture resistance of the licking bricks, as shown in Table 2. Table 2 shows that the licking bricks prepared in Examples 1-2 have the lowest moisture content, indicating that they exhibit superior moisture resistance during storage.

[0035] Table 2. Moisture-proof performance of licking bricks prepared in different experimental groups

[0036] Because licking bricks are easily exposed to sun and rain, their texture and structure must remain intact after being soaked in water and dried in the sun. Figure 3 These are images of the licking brick prepared in Example 1 of this invention before and after soaking in water. Figure 4 Images of the licking brick prepared in Comparative Example 5 of this invention before and after immersion in water; from Figure 3-4 It can be seen that the licking brick prepared in Example 1 of the present invention has a smooth appearance and a firm and solid structure. After being soaked in water and dried, it still has an intact structure and can continue to be used. In contrast, the licking brick prepared in Comparative Example 5 using calcium oxide instead of calcium silicate and bentonite components has a cracked and damaged structure after being soaked in water and dried.

[0037] 2. Effects of different experimental groups on the intestinal health of musk deer Licks prepared in Examples 1-2 and Comparative Examples 6-9 were used to investigate the effects of different microbial additive components on the intestinal immunity, intestinal inflammation, and intestinal stress index cortisol of musk deer.

[0038] The test animals were 42 female musk deer, approximately one year old, of similar weight and in good condition, without any underlying diseases. They were randomly divided into 6 groups of 6 animals each. The experiment lasted 60 days, with animals allowed free access to licking bricks and other feed. Licking bricks were provided for 60 days. In experimental group 1, animals were provided with licking bricks prepared in Example 1; in experimental group 2, animals were provided with licking bricks prepared in Example 2. Animals in the control group were not provided with any licking bricks, but other feeding conditions were the same as in experimental groups 1-2. Animals in comparative experimental groups 1, 2, 3, and 4 were provided with licking bricks prepared in comparative examples 6, 7, 8, and 9, respectively. Furthermore, the feeding and rearing methods were identical for all groups.

[0039] Detection indicators and methods 1. Testing methods for the intestinal immune capacity of the musk deer; The levels of immunoglobulin A (IgA), immunoglobulin M (IgM), and immunoglobulin G (IgG) in the collected fecal samples were detected using an enzyme-linked immunosorbent assay (ELISA) kit purchased from Jiangsu Enzyme Immunoassay Co., Ltd.

[0040] 2. Testing methods for intestinal inflammatory markers in musk deer; The fecal samples collected were analyzed using an enzyme-linked immunosorbent assay (ELISA) kit to detect the levels of bovine calprotectin, matrix metalloproteinase 9 (MMP-9), and lactoferrin (LTF). The kit was purchased from Jiangsu Enzyme Immunosorbent Assay Co., Ltd.

[0041] 3. Test method for cortisol, an intestinal stress indicator of musk deer; The collected fecal samples were tested for cortisol content using an enzyme-linked immunosorbent assay (ELISA) kit purchased from Jiangsu Enzyme Immunosorbent Assay Co., Ltd.

[0042] Test results Table 3 shows the effects of different experimental groups on the intestinal immunity of musk deer. As can be seen from Table 3, the nutritional licks prepared in Examples 1-2 significantly affected the IgA, IgG, and IgM content in musk deer feces. Compared with the blank experimental group and the control experimental groups 1-4, the IgA, IgG, and IgM content in the musk deer feces of experimental groups 1-2 was significantly reduced. The results indicate that the nutritional licks prepared in Examples 1-2 can effectively improve the intestinal immunity of captive musk deer and help reduce intestinal inflammation. Furthermore, the nutritional licks prepared in Examples 1-2 have a significantly better effect on protecting the intestinal health of captive musk deer than the nutritional licks prepared in Control Examples 6-9.

[0043] Table 3. Effects of different experimental groups on the intestinal immunity of musk deer.

[0044] Note: Different lowercase letters in the superscript of data from the same row indicate significant differences. P <0.05), different capital letters indicate highly significant differences ( P <0.01), no letter indicates no significant difference ( P >0.05); the same as in the table below.

[0045] Table 4 shows the effects of different experimental groups on intestinal inflammation markers in musk deer. As can be seen from Table 4, the licking bricks prepared using Examples 1-2 significantly affected the levels of intestinal inflammation markers such as calprotectin, MMP-9, and lactoferrin in musk deer feces. P <0.05). Compared with the blank experimental group, the contents of calprotectin, matrix metalloproteinase 9, and lactoferrin in the feces of musk deer in experimental groups 1-2 were significantly reduced. The results show that the special nutritional lick bricks for musk deer prepared in Examples 1-2 can effectively reduce the intestinal inflammatory response of captive musk deer. When the nutritional lick bricks prepared in Comparative Examples 6-9 lacked one of the microbial components or lacked yeast culture dry powder, their effect on reducing the intestinal inflammatory response of captive musk deer was significantly worse than that of the nutritional lick bricks prepared in Examples 1-2.

[0046] Table 4. Effects of different experimental groups on inflammatory markers in musk deer tracts

[0047] Under conditions of captive rearing and artificial feeding, musk deer are easily stimulated by environmental disturbances and human manipulation, leading to fright stress and an abnormally high level of cortisol, an indicator of intestinal stress. Table 5 shows the test results of cortisol, an indicator of intestinal stress in musk deer. The results indicate that providing the nutritional licks prepared in Examples 1-2 can significantly reduce the cortisol content in musk deer feces. When one of the microbial components is missing in the nutritional licks prepared in Examples 6-9, the effect of reducing the cortisol content in musk deer feces is significantly worse than that of the nutritional licks prepared in Examples 1-2. The results show that the special nutritional licks prepared in Examples 1-2 can effectively alleviate the stress response of captive musk deer. All three microbial components (Saccharomyces cerevisiae, Bacillus subtilis, and Lactobacillus plantarum) in the nutritional licks are indispensable. Furthermore, when the yeast culture powder is missing from the components, the alleviating effect of the licks on the stress response of captive musk deer is reduced.

[0048] Table 5. Effects of different experimental groups on cortisol, an intestinal stress marker in musk deer.

[0049] In summary, the licking bricks prepared using Examples 1-2 of this invention exhibit the best moisture resistance during storage, and possess a smooth appearance, compact and robust structure, and excellent formability. Even after soaking in water and drying, they retain their structural integrity and can continue to be used. In the experiment on the effects on the intestinal health of musk deer, the nutrient licking bricks specifically prepared using Examples 1-2 effectively improved the intestinal immunity of captive musk deer, significantly reduced intestinal inflammatory responses, and significantly reduced cortisol content in musk deer feces, alleviating stress responses in captive musk deer. The three microbial components in the nutrient licking bricks, combined with the yeast culture dry powder components, have a synergistic effect in improving the intestinal immunity of captive musk deer, reducing intestinal inflammatory responses, and reducing cortisol content in musk deer feces. Among all test indicators, Examples 1-2 showed the best performance and appearance, and their manufacturing method is relatively simple and inexpensive, making them suitable for preparing products related to improving the intestinal health and immunity of musk deer.

[0050] Therefore, those skilled in the art will recognize that although embodiments of the present invention have been shown and described in detail herein, many other variations or modifications conforming to the principles of the present invention can be directly determined or derived from the disclosure of the present invention without departing from the spirit and scope of the invention. Therefore, the scope of the present invention should be understood and recognized as covering all such other variations or modifications.

Claims

1. A naturally formed nutrient licking brick specifically for musk deer, characterized in that, It includes the following components: 20-35% yeast culture powder, 8-15% silicate, 15-30% bentonite, 12-25% wheat bran, 15-30% corn flour, 1-8% salt, 0.5-2% brewer's yeast, 0.5-2% Bacillus subtilis, 0.5-2% Lactobacillus plantarum, 0.02-0.1% sweetener, and 0.03%-0.2% pineapple milk flavoring.

2. The naturally formed nutrient licking brick for musk deer as described in claim 1, characterized in that, The dosage of each component is as follows: yeast culture powder 25-30%, calcium silicate 10-15%, bentonite 20-25%, wheat bran 15-20%, corn flour 15-20%, salt 2-5%, brewer's yeast 1-2%, Bacillus subtilis 1-2%, Lactobacillus plantarum 1-2%, sweetener 0.02-0.06%, and pineapple milk flavoring 0.05%-0.15%.

3. The naturally formed nutrient licking brick for musk deer as described in claim 1, characterized in that, The silicate is food-grade calcium silicate.

4. The naturally formed nutrient licking brick for musk deer as described in claim 1, characterized in that, The bentonite, sweetener, and pineapple milk flavoring are all feed-grade raw materials.

5. A naturally formed nutrient licking brick for musk deer as described in claims 1-4, characterized in that, The sweetener is one or more of sodium saccharin, thomaline, steviol glycosides, etc.

6. A method for preparing a naturally formed nutrient licking brick for musk deer as described in claims 1-5, characterized in that, Includes the following steps: Step S1: Weigh and mix calcium silicate, yeast culture powder, wheat bran, corn flour and bentonite, and stir evenly to obtain the first mixture; Step S2: Weigh and mix the salt, brewer's yeast, Bacillus subtilis, Lactobacillus plantarum, sweetener, and pineapple milk flavoring, and stir well to obtain the second mixture; Step S3: Mix the first mixture and the second mixture thoroughly to obtain the third mixture; Step S4: Add water to the third mixture and stir thoroughly until well combined; Step S5: Place the mixture into a mold, let it solidify and solidify, then demold and air dry to obtain the final product.

7. The preparation method of a naturally formed nutrient licking brick for musk deer as described in claim 6, characterized in that, In step S4, the amount of water added is 20% to 25% of the total weight of the third mixture.

8. The preparation method of a naturally formed nutrient licking brick for musk deer as described in claim 6, characterized in that, After placing the mixture into a cube mold, press the mold to shape the lick brick.

9. The preparation method of a naturally formed nutrient licking brick for musk deer as described in claim 6, characterized in that, Place in a cool, well-ventilated place for 3-10 days to allow it to naturally solidify and take shape. The licking brick is cube-shaped, with a length / width of 5-7 cm, a height of 2-4 cm, and a density of 1-1.5 g / cm³. 3 .

10. The application of the naturally shaped musk deer-specific nutritional licking bricks as described in claims 1 to 9 in the preparation of products that improve the intestinal health and immunity of musk deer.

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