A grape seed compound active composition with antioxidant efficacy and its preparation method and application

Abstract

This invention belongs to the technical field of fermentation-derived active ingredients, skin care active compositions, and topical formulations, specifically relating to a grape seed compound active composition with antioxidant effects, its preparation method, and its application. The composition uses grape seed fermentation filtrate as the main active base, and is synergistically compounded with Bifida ferment lysate and α-bisabolol. By compounding grape seed fermentation filtrate as the main component and Bifida ferment lysate and α-bisabolol as synergistic active components, a topical active composition that combines antioxidant care, gentleness, and dosage form suitability can be obtained. The composition can be formulated into topical formulations such as serums, essences, gels, sprays, lotions, facial masks, wet compresses, or lyophilized powder reconstituted solutions, and is suitable for the development of antioxidant care products.

Description

Technical Field

[0001] This invention belongs to the technical field of fermentation-derived active ingredients, skin care active compositions and topical preparations, specifically relating to a grape seed compound active composition with antioxidant effects, its preparation method and application. Background Technology

[0002] Grape seed fermentation-derived bioactive compounds, rich in polyphenolic active ingredients and fermentation metabolites, show promising applications in antioxidant skincare, skin feel improvement, and the development of topical formulations. Current research largely focuses on the grape seed fermentation broth itself, screening of fermentation strains, or activity evaluation of single fermentation raw materials. However, systematic research on the compounding methods, dosage form adaptations, and composition construction of grape seed fermentation filtrate in topical applications remains relatively insufficient.

[0003] On the other hand, Bifida ferment lysate, as a fermentation-derived active ingredient, has been widely used in functional skincare products. It originates from the lysate active components after Bifidobacterium fermentation and typically contains amino acids, polysaccharides, vitamins, and other fermentation metabolites. Preferably, it may also contain antioxidant active ingredients such as ergothioneine. Alpha-bisabolol, on the other hand, is a low-addition active ingredient with soothing and conditioning potential.

[0004] However, existing technologies related to active ingredients derived from grape seed fermentation mostly focus on single active ingredients, basic formulation systems, or the fermentation process itself, lacking formulation schemes that use grape seed fermentation filtrate as the main active base, with Bifida ferment lysate and α-bisabolol as small synergistic components. In particular, there is a lack of systematic designs that address how to achieve enhanced antioxidant effects while maintaining gentleness and dosage form compatibility within the same topical system. Summary of the Invention

[0005] In view of the problems that existing technologies have relatively simple compounding methods for active compositions derived from grape seed fermentation, insufficient activity levels, and need to improve the adaptability of external dosage forms, this invention provides a grape seed compound active composition with antioxidant effects, its preparation method, and its application.

[0006] A grape seed compound active composition with antioxidant effects, comprising the following components by weight: 5-30 parts grape seed fermentation filtrate, 0.1-8 parts Bifida ferment lysate, 0.01-1.0 parts α-bisabolol, 1-20 parts glycerol, 1-10 parts 1,3-propanediol, 0.5-3 parts betaine, 0.1-1 parts solubilizer, 0-1.5 parts xanthan gum, 0-0.2 parts chelating agent, 0.01-1.0 parts pH adjuster, and deionized water to a total of 100 parts.

[0007] This invention uses grape seed fermentation filtrate as the main active base. Unlike directly using the fermentation stock, the grape seed fermentation filtrate is preferably obtained by centrifugation to remove bacterial cells and suspended particles, followed by membrane filtration, making it more suitable for further compounding and constructing topical compositions. As the main active base, the grape seed fermentation filtrate primarily provides polyphenolic active ingredients and fermentation-derived active components, which are the main source of the antioxidant and skincare effects of the compositions of this invention.

[0008] Preferably, the grape seed fermentation filtrate is derived from the filtrate or its concentrate obtained by centrifuging grape seed fermentation broth to remove bacteria and insoluble particles, followed by membrane filtration.

[0009] Preferably, the Bifida ferment lysate is an active component derived from the fermentation lysis product of Bifidobacterium.

[0010] Preferably, the Bifida ferment lysate contains ergothioneine.

[0011] Based on grape seed fermentation filtrate, this invention further introduces Bifida ferment lysate as a co-fermentation source active component. The Bifida ferment lysate is preferably derived from the active raw material obtained after Bifidobacterium fermentation, followed by lysis, removal of insoluble components, and post-processing, and preferably contains ergothioneine. The introduction of Bifida ferment lysate does not replace the grape seed fermentation filtrate, but rather adds it as a low to medium proportion of co-fermentation source active component to expand the fermentation-derived activity levels of the composition and to improve the antioxidant protection performance of the compound system.

[0012] Preferably, the weight ratio of the grape seed fermentation filtrate, the Bifida ferment lysate, and α-bisabolol is (20~80):(2~15):(0.2~1.5). The co-solvent is preferably glycerol, 1,3-propanediol, PEG-40 hydrogenated castor oil, or a combination thereof.

[0013] This invention also introduces α-bisabolol as a small amount of synergistic active ingredient. The amount of α-bisabolol added is significantly lower than that of the basic active ingredient in grape seed fermentation filtrate. Its main function is not to provide the main antioxidant capacity alone, but to help improve the soothing and care properties of the composition, reduce the compatibility pressure of the highly active system on skin application scenarios, and help improve the overall suitability for use.

[0014] This invention particularly emphasizes the complex relationship between grape seed fermentation filtrate, Bifida ferment lysate, and α-bisabolol: grape seed fermentation filtrate is the main active base, Bifida ferment lysate is a synergistic active component derived from fermentation, and α-bisabolol is a small amount of synergistic soothing component. Compared to a system using only grape seed fermentation filtrate, the addition of Bifida ferment lysate further enhances the composition's free radical scavenging performance; further addition of α-bisabolol further improves the composition's comprehensive antioxidant care and topical compatibility, thus forming a grape seed complex active composition with antioxidant effects.

[0015] Preferably, the method for preparing grape seed fermentation filtrate is as follows: take grape seed fermentation broth, centrifuge it to remove bacterial cells and larger particulate matter, and collect the supernatant; then filter the supernatant through a 0.22μm aqueous filter membrane to obtain grape seed fermentation filtrate.

[0016] The preparation method of the grape seed compound active composition with antioxidant effects includes the following steps: (1) The grape seed fermentation broth was centrifuged and membrane filtered to obtain grape seed fermentation filtrate; (2) The grape seed fermentation filtrate is mixed with the Bifida ferment lysate to form the main active aqueous phase; (3) Prepare α-bisabolol as a synergistic active addition phase; (4) Add the synergistic active phase obtained in step (3) to the main active aqueous phase obtained in step (2), and then add the remaining components. After mixing and homogenizing, a grape seed compound active composition with antioxidant effects is obtained.

[0017] Preferably, in step (3), α-bisabolol is added to the cosolvent in advance to form a synergistic active addition phase.

[0018] The antioxidant active composition is used in the preparation of topical products with antioxidant care, soothing care or skin condition improvement effects.

[0019] Preferably, the topical product is formulated as an essence, essence water, gel, spray, lotion, mask liquid, wet compress liquid, or freeze-dried powder reconstituted solution.

[0020] The beneficial effects of this invention include: (1) Using grape seed fermentation filtrate as the main active base, a compound active composition with plant fermentation-derived active components as the core was constructed; (2) By introducing a small proportion of Bifida ferment lysate, the fermentation-derived activity level of the composition can be enriched, and preferably the antioxidant care performance can be further enhanced by the ergothionein contained therein. (3) By introducing a small amount of α-bisabolol, it is beneficial to improve the soothing and nursing properties and compatibility of the composition; (4) By combining the main active aqueous phase and the synergistic active phase in a stepwise manner, it is beneficial to form a composition suitable for various external application forms such as serum, essence water, gel, spray, lotion, mask liquid and wet compress liquid; (5) The irritation of chicken embryo chorioallantoic membrane and the hemolytic activity of sheep blood plate demonstrate that the composition of the present invention has a good basis for external use safety under the tested conditions. Attached Figure Description

[0021] Figure 1 This is a flowchart illustrating the overall preparation process of the grape seed compound active composition with antioxidant effects according to the present invention. Figure 2 The figure shows the evaluation results of the irritation of the chicken embryo chorioallantoic membrane in Example 6 of the present invention; in the figure, (a)~(d) are the initial state of each treatment group at 0 min, and (e)~(h) are the results of the corresponding treatment groups after 5 min; among them, (c) and (g) are 5% sample treatment groups, and (d) and (h) are 10% sample treatment groups.

[0022] Figure 3 This is a graph showing the results of the hemolytic activity evaluation of sheep blood platelets in Example 7 of the present invention. Detailed Implementation

[0023] The present invention will be further described below with reference to specific embodiments. It should be understood that these embodiments are for illustrative purposes only and are not intended to limit the scope of protection of the present invention. Unless otherwise specified, the raw materials, reagents and instruments used can be products from conventional sources in the art.

[0024] The grape seed fermentation filtrate used in this invention is the main active base actually used in the embodiments of this patent, and its preparation method is as follows: Raw material and substrate preparation: Select dry grape seed coarse powder, crush it to about 60 mesh, add purified water at a mass-volume ratio of 1:12, soak at room temperature for 20 min; heat treat at 90℃ for 15 min; homogenize at high shear 10000 r / min for 2 min, and cool to 37℃ to obtain a homogeneous grape seed fermentation substrate.

[0025] Preparation of strains and seed culture: Lactobacillus plantarum (CICC24988) and Lactobacillus fermentum (CICC20176) from the China Industrial Microbial Culture Collection Center were revived and activated, respectively, and then subjected to primary and secondary expansion cultures. The viable cell counts in both cultures remained stable at 1×10⁻⁶. 9 CFU / mL.

[0026] Fermentation conditions (sequential inoculation of two bacteria, two stages): Stage 1: 4% *Lactobacillus plantarum* inoculated, fermented at 36℃ for 12 hours; Stage 2: 2% *Lactobacillus mucilaginosus* added, fermented at 36℃ for another 24 hours; Stirring conditions: 120 rpm for a 5L tank or approximately 0.5 W / L based on tank power; Dynamic determination of fermentation endpoint: pH drops to approximately 3.95, and the total phenol and DPPH free radical scavenging rates reach a plateau.

[0027] Post-processing: The fermentation broth is centrifuged at 5000 r / min for 20 min to remove bacterial cells and large particles, and then filtered through a 0.22 μm aqueous filter membrane to obtain grape seed fermentation filtrate, which can be directly used for the preparation of the compound composition of this patent or for concentration for later use.

[0028] Quality indicators: Total phenols approximately 5.32 g / L, DPPH removal rate approximately 93%, total phenol retention rate approximately 86% and DPPH retention rate approximately 91% after 90 days of storage at 4°C protected from light.

[0029] The grape seed fermentation filtrate is the main active base for the compound Bifida ferment lysate and α-bisabolol in this embodiment of the invention, ensuring full release of activity, batch consistency and compatibility with subsequent topical compositions.

[0030] Example 1: Preparation of a basic antioxidant active composition Grape seed fermentation broth was centrifuged at 8000g for 20 minutes to remove bacterial cells and larger particulate matter, and the supernatant was collected. The supernatant was then filtered through a 0.22μm aqueous filter membrane to obtain grape seed fermentation filtrate.

[0031] By weight, take 12 parts of grape seed fermentation filtrate, 2 parts of Bifida ferment lysate, 0.10 parts of α-bisabolol, 5 parts of glycerol, 4 parts of 1,3-propanediol, 1 part of betaine, 0.6 parts of solubilizer, 0.18 parts of xanthan gum, 0.05 parts of disodium EDTA, and 0.15 parts of citric acid / sodium citrate buffer system, and add deionized water to make up to 100 parts.

[0032] The process involves first mixing grape seed fermentation filtrate with Bifida ferment lysate to form the main active aqueous phase; then dissolving α-bisabolol in a co-solvent to form a synergistic active addition phase, wherein the co-solvent is glycerol, to ensure the uniform dispersion and stability of α-bisabolol in the aqueous phase; finally, the synergistic active addition phase is slowly added to the main active aqueous phase, and the remaining excipients are added sequentially and homogenized to obtain a grape seed compound active composition with antioxidant effects.

[0033] Example 2: Synergistic effect of compound formulation to enhance antioxidant performance Four groups of samples were set up for comparison: Group A was the basic system containing only grape seed fermentation filtrate; Group B was the system of grape seed fermentation filtrate + Bifida ferment lysate; Group C was the system of grape seed fermentation filtrate + α-bisabolol; and Group D was the synergistic compound system of grape seed fermentation filtrate + Bifida ferment lysate + α-bisabolol.

[0034] Group A is a basic system containing only grape seed fermentation filtrate; by weight, take 12 parts grape seed fermentation filtrate, 5 parts glycerol, 4 parts 1,3-propanediol, 1 part betaine, 0.18 parts xanthan gum, 0.05 parts disodium EDTA, and 0.15 parts citric acid / sodium citrate buffer system, and add deionized water to make up to 100 parts.

[0035] Group B is a system consisting of grape seed fermentation filtrate and Bifida ferment lysate. By weight, take 12 parts of grape seed fermentation filtrate, 2 parts of Bifida ferment lysate, 5 parts of glycerol, 4 parts of 1,3-propanediol, 1 part of betaine, 0.18 parts of xanthan gum, 0.05 parts of disodium EDTA, and 0.15 parts of citric acid / sodium citrate buffer system, and add deionized water to make up to 100 parts.

[0036] Group C is a system of grape seed fermentation filtrate + α-bisabolol; by weight, take 12 parts of grape seed fermentation filtrate, 0.10 parts of α-bisabolol, 5 parts of glycerol, 4 parts of 1,3-propanediol, 1 part of betaine, 0.6 parts of solubilizer, 0.18 parts of xanthan gum, 0.05 parts of disodium EDTA, and 0.15 parts of citric acid / sodium citrate buffer system, and add deionized water to make up to 100 parts.

[0037] Group D is a synergistic compound system of grape seed fermentation filtrate, Bifida ferment lysate, and α-bisabolol. By weight, 12 parts grape seed fermentation filtrate, 2 parts Bifida ferment lysate, 0.10 parts α-bisabolol, 5 parts glycerol, 4 parts 1,3-propanediol, 1 part betaine, 0.6 parts solubilizer, 0.18 parts xanthan gum, 0.05 parts disodium EDTA, and 0.15 parts citric acid / sodium citrate buffer system were added to bring the total to 100 parts with deionized water. The preparation method is the same as in Example 1.

[0038] The antioxidant performance was evaluated using DPPH free radical scavenging rate. The results showed that the DPPH free radical scavenging rate was 61.4% in group A, increased to 69.2% in group B, 64.8% in group C, and further increased to 76.5% in group D. These results indicate that when grape seed fermentation filtrate is used as the main active base, the addition of Bifida ferment lysate significantly enhances the antioxidant performance, and the further introduction of α-bisabolol further improves the overall antioxidant protection performance of the composition.

[0039] The above results demonstrate that the present invention is not simply a combination of multiple active ingredients, but rather, based on grape seed fermentation filtrate as the main active base, a better synergistic compound system is formed by introducing a small proportion of Bifida ferment lysate and α-bisabolol, thereby further improving the antioxidant care performance of the composition.

[0040] Example 3: Effect of different amounts of Bifida ferment lysate added on synergistic effect With the grape seed fermentation filtrate fixed at 12 parts and α-bisabolol fixed at 0.10 parts, the changes in DPPH free radical scavenging rate of the composition were investigated when the amount of Bifida ferment lysate added was 0.5 parts, 1 part, 2 parts and 4 parts.

[0041] The results showed that as the amount of Bifida ferment lysate added increased from 0.5 parts to 2 parts, the DPPH free radical scavenging rate of the composition gradually increased; when the amount added continued to increase, the increase tended to level off. This indicates that Bifida ferment lysate, as a synergistic active component, can achieve good synergistic effects in the range of low to medium addition amounts.

[0042] Example 4: Effect of different α-bisabolol addition methods on the uniformity of compound formulation Three methods were investigated: direct addition of α-bisabolol, pre-dissolution of polyol, and pre-dispersion of co-solvent system.

[0043] 1. Direct addition method: Add α-bisabolol solid directly to the main active aqueous phase. Stir with a magnetic stirrer or mechanical stirrer at room temperature for 30 minutes to attempt dispersion.

[0044] 2. Polyol pre-dissolution method: Mix α-bisabolol with glycerol or 1,3-propanediol at a weight ratio of approximately 1:5, and stir at room temperature for 20 minutes until completely dissolved to form a homogeneous liquid. Slowly add this liquid to the main active aqueous phase while stirring slowly for 15 minutes until fully dispersed.

[0045] 3. Pre-dispersion method using a solubilizing system (preferred): Dissolve α-bisabolol in a solubilizing agent, glycerol, to form a synergistic active phase. Homogenize using a high-speed stirrer for 2 minutes to obtain a transparent and homogeneous dispersion. Slowly add the main active aqueous phase while homogenizing for 2 minutes to obtain a homogeneous and stable compound system.

[0046] The results showed that the direct addition method was prone to local aggregation at room temperature; the polyol pre-dissolution method improved its distribution uniformity; and the pre-dispersion method using a co-solvent system performed better in transparent serum and essence water systems. The DPPH free radical scavenging effects of the direct addition method and the polyol pre-dissolution method were 72% and 74%, respectively, which were lower than those of the co-solvent system, indicating that different α-bisabolol addition methods also affected the DPPH free radical scavenging effect.

[0047] Therefore, it is preferable to pre-dissolve or pre-disperse α-bisabolol before adding it to the main active system of grape seed fermentation filtrate-Bifida ferment lysate, so as to facilitate the formation of a uniform and stable synergistic compound composition.

[0048] Example 5: Basic Dosage Form Adaptation Example Based on the synergistic compounding system of this invention, various dosage forms can be further formulated. The thickener content in the serum-type sample is controlled at 0.05~0.30 parts; the thickener content in the water-type sample is controlled at 0~0.05 parts; the thickener content in the gel-type sample is controlled at 0.30~1.20 parts; and for the spray-type sample, it is preferable to reduce viscosity and control the solubilizing ratio.

[0049] The results showed that the synergistic compound system, with grape seed fermentation filtrate as the main component and Bifida ferment lysate and α-bisabolol as auxiliary components, has good compatibility with a variety of conventional topical excipients and can meet the development needs of different topical formulations.

[0050] Example 6: Evaluation of Irritation of Chicken Embryo Villi Allantoic Membrane The composition of this invention was used to conduct an irritation test on the chorioallantoic membrane of chicken embryos. After 5 minutes of treatment, compared with the negative control group, no obvious bleeding, vascular rupture or coagulation was observed in the sample group, and the vascular network remained intact overall.

[0051] The results indicate that the composition of the present invention did not exhibit a significant tendency to cause irritation under the tested conditions, and has a safety basis for further development of topical care products.

[0052] Example 7: Evaluation of hemolytic activity of sheep blood platelets The composition of the present invention and a positive control were simultaneously applied to sheep blood agar plates. The positive control showed a clear hemolytic zone, while no clear zone or discolored hemolytic area was observed in the group treated with the composition of the present invention.

[0053] This indicates that the composition of the present invention did not exhibit significant hemolytic activity under the experimental conditions described, demonstrating good preliminary safety.

[0054] Example 8: Example of a serum-type composition By weight, 15 parts grape seed fermentation filtrate, 2.5 parts Bifida ferment lysate, 0.12 parts α-bisabolol, 5 parts glycerin, 5 parts 1,3-propanediol, 1 part betaine, 0.20 parts xanthan gum, 0.8 parts solubilizer, 0.05 parts chelating agent, and 0.12 parts pH adjuster were added, and deionized water was added to bring the total to 100 parts to prepare an essence-type composition. This sample has a light skin feel and is suitable for the development of antioxidant skincare essence products.

[0055] Example 9: Example of an essence-type composition By weight, 10 parts of grape seed fermentation filtrate, 1.5 parts of Bifida ferment lysate, 0.08 parts of α-bisabolol, 3 parts of glycerol, 3 parts of 1,3-propanediol, 0.5 parts of solubilizer, and 0.10 parts of buffer system were added, and deionized water was added to bring the total to 100 parts to prepare an essence-type composition. This sample has good flowability and is suitable for the development of pat-on and wet-application products.

[0056] Example 10: Example of a gel-type composition By weight, 14 parts of grape seed fermentation filtrate, 2 parts of Bifida ferment lysate, 0.10 parts of α-bisabolol, 4 parts of glycerol, 0.35 parts of carbomer, 0.7 parts of solubilizer, and an appropriate amount of neutralizing agent were added, and deionized water was added to make up to 100 parts to obtain a gel-type composition. This sample has good film-forming properties and is suitable for the development of local soothing care products.

[0057] Example 11: Example of a spray-type composition By weight, 8 parts of grape seed fermentation filtrate, 1 part of Bifida ferment lysate, 0.05 parts of α-bisabolol, 2 parts of glycerol, 2 parts of 1,3-propanediol, 0.4 parts of solubilizer, and 0.08 parts of pH adjuster were added, and deionized water was added to bring the total to 100 parts to prepare a spray-type composition. This sample exhibits good spray uniformity and is suitable for developing lightweight antioxidant care sprays.

[0058] Comparative Example 1: Basic system containing only grape seed fermentation filtrate The difference between this comparative example and Example 1 is that the external application sample was constructed using only grape seed fermentation filtrate as the active base, without the addition of Bifida ferment lysate and α-bisabolol. The results showed that although this system exhibited some antioxidant activity, its DPPH radical scavenging rate was lower than that of the synergistic compound system of this invention.

[0059] Comparative Example 2: Compound system without Bifida ferment lysate The difference between this comparative example and Example 1 is that α-bisabolol was added to the grape seed fermentation filtrate, but not Bifida ferment lysate. The results showed that its antioxidant performance was higher than the basic system containing only grape seed fermentation filtrate, but still lower than the three-activity synergistic compound system of this invention.

[0060] Comparative Example 3: Compound system without α-bisabolol The difference between this comparative example and Example 1 is that Bifida ferment lysate was added to the grape seed fermentation filtrate instead of α-bisabolol. Results showed that its antioxidant performance was superior to the basic system containing only grape seed fermentation filtrate, but its overall compatibility was inferior to the three-activity synergistic compound system of this invention.

[0061] Comparative Example 4: The difference between this comparative example and Example 1 is as follows: The formula contained 6 parts grape seed fermentation filtrate, 0.5 parts Bifida ferment lysate, and 0.02 parts α-bisabolol, with the remaining components being the same as in Example 1. Results: The DPPH free radical scavenging rate was approximately 63%, but the spray uniformity was slightly poor, and the local activity distribution was not as uniform as the standard formula.

[0062] Comparative Example 5: The difference between this comparative example and Example 1 is that the formula contains 15 parts grape seed fermentation filtrate, 5 parts Bifida ferment lysate, and 0.5 parts α-bisabolol, while the remaining components are the same as in Example 1. Results: The DPPH free radical scavenging rate was about 78%, and the antioxidant activity was enhanced, but the spray viscosity increased and the uniformity decreased.

[0063] Performance testing The total phenol content can be determined by the Folin-Ciocalteu method, with gallic acid as the standard to establish a standard curve, and the results are expressed in g / L.

[0064] The DPPH free radical scavenging rate can be determined by spectrophotometry. The free radical scavenging rate is calculated by comparing the change in absorbance before and after sample treatment.

[0065] The chicken embryo chorioallantoic membrane irritation test can be used to evaluate the topical irritation tendency of a composition under the test conditions.

[0066] The sheep blood plate hemolytic test can be used to evaluate whether a composition has significant hemolytic activity under the test conditions.

[0067] The results are shown in Table 1.

[0068] Table 1 Comparison of DPPH free radical scavenging rates of compositions under different compounding methods Group Main active components DPPH free radical scavenging rate (%) illustrate Group A Grape seed fermentation filtrate 61.4 basic system Group B Grape seed fermentation filtrate + Bifida ferment lysate 69.2 Significantly enhanced synergy Group C Grape seed fermentation filtrate + α-bisabolol 64.8 There has been some improvement. Group D Grape seed fermentation filtrate + Bifida ferment lysate + α-bisabolol 76.5 Best overall effect Example 4 Direct addition method 72 The uniformity is not as good as Group D Example 4 Polyol pre-dissolution method 74 The uniformity is not as good as Group D Comparative Example 4 The dosage of the three components is too low. 63 The uniformity is not as good as Group D Comparative Example 5 The dosage of the three components is too high. 78 The uniformity is not as good as Group D Any equivalent substitutions or conventional adjustments made by those skilled in the art to the source of grape seed fermentation filtrate, the type of lysate from Bifida ferment lysate, the method of adding α-bisabolol, the composition of dosage form excipients, and the application direction without departing from the spirit and essence of this invention shall fall within the protection scope of this invention.

Claims

1. A grape seed compound active composition with antioxidant effects, characterized in that, The product comprises, by weight, the following components: 5-30 parts grape seed fermentation filtrate, 0.1-8 parts Bifida ferment lysate, 0.01-1.0 parts α-bisabolol, 1-20 parts glycerol, 1-10 parts 1,3-propanediol, 0.5-3 parts betaine, 0.1-1 parts solubilizer, 0-1.5 parts xanthan gum, 0-0.2 parts chelating agent, and 0.01-1.0 parts pH adjuster, to be made up to 100 parts with deionized water.

2. The grape seed compound active composition with antioxidant effects as described in claim 1, characterized in that, The grape seed fermentation filtrate is derived from the grape seed fermentation broth after centrifugation to remove bacteria and insoluble particles, followed by membrane filtration to obtain the filtrate or its concentrate.

3. The grape seed compound active composition with antioxidant effects as described in claim 1, characterized in that, The Bifida ferment lysate is an active component derived from the fermentation lysis product of Bifidobacterium.

4. The grape seed compound active composition with antioxidant effects as described in claim 3, characterized in that, The bifida ferment lysate contains ergothioneine; the cosolvent is preferably glycerol, 1,3-propanediol, PEG-40 hydrogenated castor oil, or a combination thereof.

5. The grape seed compound active composition with antioxidant effects as described in claim 1, characterized in that, The weight ratio of the grape seed fermentation filtrate, the Bifida ferment lysate, and α-bisabolol is (20~80):(2~15):(0.2~1.5).

6. The grape seed compound active composition with antioxidant effects as described in claim 1, characterized in that, The preparation method of grape seed fermentation filtrate is as follows: take grape seed fermentation broth, centrifuge it to remove bacterial cells and larger particulate matter, and collect the supernatant; then filter the supernatant through a 0.22μm aqueous filter membrane to obtain grape seed fermentation filtrate.

7. A method for preparing a grape seed compound active composition with antioxidant effects as described in any one of claims 1 to 6, characterized in that, Includes the following steps: (1) Take grape seed fermentation liquid, centrifuge it to remove bacteria and larger particulate matter, collect the supernatant, and then filter the supernatant through an aqueous filter membrane to obtain grape seed fermentation filtrate; (2) Take grape seed fermentation filtrate, Bifida ferment lysate, α-bisabolol, glycerol, 1,3-propanediol, betaine, solubilizer, xanthan gum, disodium EDTA, citric acid / sodium citrate buffer system and deionized water by weight for later use. (3) The grape seed fermentation filtrate is mixed with the Bifida ferment lysate to form the main active aqueous phase; (4) Dissolve or disperse α-bisabolol in a co-solvent to form a synergistic active addition phase, wherein the co-solvent is one or more of glycerol, 1,3-propanediol, and PEG-40 hydrogenated castor oil; (5) The synergistic active phase obtained in step (4) is slowly added to the main active aqueous phase obtained in step (3), and then glycerol, 1,3-propanediol, betaine, xanthan gum, disodium EDTA, citric acid / sodium citrate buffer system and deionized water are added in sequence. After mixing and homogenizing, a grape seed compound active composition with antioxidant effect is obtained.

8. The method for preparing the grape seed compound active composition with antioxidant effects as described in claim 7, characterized in that, Step (3) α-bisabolol is added to the cosolvent in advance to form a synergistic active addition phase.

9. The use of the antioxidant active composition according to any one of claims 1 to 6 in the preparation of a topical product having antioxidant care, soothing care or skin condition improvement effects.

10. The use of the antioxidant active composition of claim 9 in the preparation of a topical product having antioxidant care, soothing care, or skin condition improvement effects, characterized in that, The topical products are formulated as serums, essences, gels, sprays, lotions, mask liquids, wet compress liquids, or freeze-dried powder reconstituted solutions.

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