A sample extraction device for microglial tissue

Abstract

The application discloses a sample extraction device for microglial cell tissues and belongs to the technical field of sample extraction devices for cell tissues. In order to solve the problem that when cell tissues at different liquid levels or at different positions of the same liquid level are extracted, a set of extraction systems is shared, and cell tissues at different levels are mixed together, thereby affecting subsequent observation and research work on the cells, the length of the lengthening cylinder is adjusted by rotating the driving cylinder, so that the plurality of extraction assemblies correspond to different depths, and the suction and discharge actions of the air bag can be matched, so that microglial cell tissues at different depths in the separation box can be extracted at the same time, and the extraction efficiency is improved. In addition, when the cell tissues are sucked in, the blocking ring moves up and contacts the mounting ring, the fixed cylinder is effectively blocked by the sealing plate and the sealing ring, the tissue fluid is prevented from entering the air bag and causing pollution, the sealing element between the fixed cylinder and the lengthening cylinder ensures the sealing property during sliding, gas leakage is avoided, the formation of the extraction negative pressure is affected, and the extraction effect is ensured.

Description

Technical Field

[0001] This invention relates to the field of sample extraction equipment for cell tissues, and more specifically, to a sample extraction device for microglial cell tissues. Background Technology

[0002] Cells are the basic structural and functional units of living organisms. It is known that all organisms except viruses are composed of cells. However, viral life activities can only be manifested in cells. Generally speaking, most microorganisms such as bacteria and protozoa are composed of a single cell, i.e., single-celled organisms. Higher plants and higher animals are multicellular organisms. Cell samples need to be extracted using extraction devices.

[0003] Chinese patent document CN212833775U discloses a sample extraction device for microglial tissue, including a base plate, a side plate mounted on the top of the base plate, a placement plate mounted on the side wall of the side plate, and a heating block mounted on the top of the placement plate, which solves the problem of inconvenience in extracting cell tissue.

[0004] However, existing extraction devices use a single extraction system when extracting cell tissues from different liquid levels or different locations within the same liquid level. This can lead to cell tissues from different layers being mixed together, affecting subsequent observation and research of the cells.

[0005] Therefore, in order to solve such problems, we propose a sample extraction device for microglial tissue. Summary of the Invention

[0006] The purpose of this invention is to provide a sample extraction device for microglial cell tissue, which aims to solve the problem in the above-mentioned background art that when extracting cell tissues at different liquid levels or different positions at the same liquid level, using the same extraction system will cause cell tissues at different levels to be mixed together, affecting subsequent observation and research of cells.

[0007] To achieve the above objectives, the present invention provides the following technical solution: a sample extraction device for microglial tissue, comprising a base, wherein a vertical plate is fixedly connected to the side wall of the base, and an installation component is provided on the side wall of the middle part of the vertical plate;

[0008] The mounting assembly includes a second mounting plate fixedly connected to the side wall of the vertical plate. The side wall of the middle portion of the second mounting plate has a plurality of through holes evenly distributed. Two mounting seats are fixedly connected to the upper end of the second mounting plate. A pressure plate is movably connected to the inner wall of the mounting seats. An airbag is fixedly connected to the lower end of the pressure plate. A plurality of air holes are evenly distributed at the lower end of the airbag. The lower end of the airbag is fixedly connected to the second mounting plate. The air holes are located directly above the through holes. A driving assembly is provided at the upper end of the mounting seat. An extraction assembly is provided at the lower end of the middle portion of the second mounting plate.

[0009] The extraction assembly includes a fixed cylinder fixedly connected to the lower port of the through hole, an extension cylinder slidably connected to the inner wall of the fixed cylinder, a fixed ring fixedly connected to the side wall of the extension cylinder outside the fixed cylinder, a drive cylinder movably sleeved on the side wall of the fixed ring, the inner wall of the upper end of the drive cylinder being threadedly connected to the fixed cylinder, and a sealing assembly provided on the inner wall of the upper end of the fixed cylinder.

[0010] Preferably, the sealing assembly includes a mounting ring fixedly connected to the inner wall of the fixed cylinder, and a plurality of mounting posts are uniformly fixedly connected to the lower end of the mounting ring, with sealing rings slidably connected to the side walls of the mounting posts.

[0011] Preferably, the drive assembly includes a third mounting plate fixedly connected to the upper end of the mounting base, a rotary drive component is fixedly connected to the upper end of the third mounting plate, a first gear is fixedly connected to the output end of the rotary drive component, a second gear is meshed with the side wall of the first gear, a threaded column is fixedly connected to the lower end of the second gear, a drive frame is threadedly connected to the side wall of the threaded column, and the lower end of the drive frame is fixedly connected to the pressure plate.

[0012] Preferably, the inner wall at the lower port of the fixed cylinder and the outer wall at the upper port of the extended cylinder are both provided with sealing elements.

[0013] Preferably, the upper surface of the sealing ring is provided with a first mounting groove and a second mounting groove, the inner wall of the first mounting groove is provided with a sealing plate, and the inner wall of the second mounting groove is provided with a sealing ring.

[0014] Preferably, a gap is left between the outer wall of the sealing ring and the inner wall of the fixing cylinder.

[0015] Preferably, the two non-adjacent side walls of the pressure plate are respectively fixedly connected to a first limiting block, and the inner side wall of the mounting base is provided with a first limiting groove, and the first limiting block is slidably connected to the inner wall of the first limiting groove.

[0016] Preferably, a liquid dispensing hopper is provided on the upper side wall of the vertical plate, and a fastening plate is fixedly connected to the side wall of the liquid dispensing hopper. One end of the fastening plate is fixedly connected to the vertical plate. A leakage pipe is provided at the lower end of the liquid dispensing hopper. A first mounting plate is fixedly connected to the side wall of the middle part of the vertical plate. A heating block is provided at the upper end of the first mounting plate. A leakage box is provided at the upper end of the heating block. The lower end of the leakage pipe is located in the leakage box. An outlet pipe is provided on the side wall at the lower end of the leakage box.

[0017] Preferably, a linear drive component is provided at the upper end of the base, and a mounting frame is fixedly connected to the output end of the linear drive component. A second rotary drive component is fixedly connected to the inner wall of the mounting frame. The output end of the second rotary drive component penetrates through the upper surface of the mounting frame. A separation box is fixedly connected to the output end of the second rotary drive component. A fourth mounting plate is provided on each of the non-adjacent side walls of the mounting frame. The lower end of the fourth mounting plate is fixedly connected to the upper surface of the base.

[0018] Preferably, the fourth mounting plate has a second limiting groove on its side wall, and a second limiting block is slidably connected to the inner wall of the second limiting groove. The second limiting block is fixedly connected to the side wall of the mounting frame.

[0019] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0020] 1. Achieve simultaneous extraction at multiple depths: By adjusting the length of the extended cylinder through the rotating drive cylinder, multiple extraction components can be made to correspond to different depths. Combined with the suction and discharge action of the airbag, small glial cell tissues at different depths in the separation chamber can be extracted simultaneously, improving extraction efficiency.

[0021] 2. Prevent tissue fluid contamination: In the sealing assembly, when cells and tissues are aspirated, the sealing ring moves upward and contacts the mounting ring. The sealing plate and sealing ring effectively seal the fixing cylinder, preventing tissue fluid from entering the airbag and causing contamination.

[0022] 3. Stable and reliable operation: The pressure plate is slidably connected to the first limiting groove of the mounting base through the first limiting block, and the mounting frame is slidably connected to the second limiting groove of the fourth mounting plate through the second limiting block, which improves the stability of the movement of the pressure plate and the mounting frame, and ensures that the extraction process is accurate and controllable.

[0023] 4. Excellent sealing: The seal between the fixed cylinder and the extended cylinder ensures a tight seal during the sliding process, preventing gas leakage from affecting the formation of the extraction negative pressure and ensuring the extraction effect. Attached Figure Description

[0024] Figure 1 This is a three-dimensional illustration of the present invention. Figure 1 ;

[0025] Figure 2 This is a three-dimensional illustration of the present invention. Figure 2 ;

[0026] Figure 3 This is a three-dimensional exploded view of the driving component in this invention;

[0027] Figure 4 This is a three-dimensional exploded view of the mounting components in this invention. Figure 1 ;

[0028] Figure 5 This is a three-dimensional structural diagram of the airbag in this invention;

[0029] Figure 6 This is a three-dimensional structural diagram of the mounting frame in this invention;

[0030] Figure 7 This is a three-dimensional exploded view of the mounting components in this invention. Figure 1 ;

[0031] Figure 8 This is a three-dimensional exploded view of the components extracted in this invention;

[0032] Figure 9 This is a cross-sectional view of the components extracted in this invention;

[0033] Figure 10 This is a three-dimensional exploded view of the sealing component in this invention.

[0034] Legend:

[0035] 1. Base; 11. Vertical plate; 12. Liquid dispensing hopper; 13. Fastening plate; 14. Leakage pipe; 15. First mounting plate; 16. Heating block; 17. Leakage tank; 18. Discharge pipe; 2. Mounting assembly; 21. Second mounting plate; 22. Through hole; 23. Mounting base; 231. First limiting groove; 24. Pressure plate; 241. First limiting block; 25. Airbag; 251. Air hole; 26. Drive assembly; 261. Third mounting plate; 262. Rotary drive component one; 263. First gear; 264. Second gear; 265. Thread 266. Column; 27. Drive frame; 28. Extraction assembly; 271. Fixing cylinder; 272. Extension cylinder; 273. Fixing ring; 274. Drive cylinder; 275. Seal; 28. Sealing assembly; 281. Mounting ring; 282. Mounting column; 283. Sealing ring; 284. First mounting groove; 285. Sealing plate; 286. Second mounting groove; 287. Sealing ring; 3. Linear drive component; 31. Mounting frame; 32. Rotary drive component two; 33. Fourth mounting plate; 34. Second limiting groove; 35. Second limiting block; 4. Separation box. Detailed Implementation

[0036] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0037] To address the issue of using a single extraction system when extracting cells from different liquid levels or different locations within the same liquid level, which can lead to the mixing of cells from different layers and affect subsequent cell observation and research, please refer to [link to relevant documentation]. Figures 1-5The following preferred technical solutions are provided.

[0038] One embodiment of the present invention: a sample extraction device for microglial tissue, including a base 1, a vertical plate 11 fixedly connected to the side wall of the base 1, and an installation component 2 provided on the side wall of the middle part of the vertical plate 11;

[0039] Mounting assembly 2 includes a second mounting plate 21 fixedly connected to the side wall of the vertical plate 11. The side wall of the middle part of the second mounting plate 21 is evenly provided with multiple through holes 22. The upper end of the second mounting plate 21 is fixedly connected with two mounting seats 23. The inner wall of the mounting seat 23 is movably connected with a pressure plate 24. The lower end of the pressure plate 24 is fixedly connected with an airbag 25. The lower end of the airbag 25 is evenly provided with multiple air holes 251. The lower end of the airbag 25 is fixedly connected to the second mounting plate 21. The air holes 251 are located directly above the through holes 22. The upper end of the mounting seat 23 is provided with a driving assembly 26. The lower end of the middle part of the second mounting plate 21 is provided with an extraction assembly 27.

[0040] The extraction component 27 includes a fixed cylinder 271 fixedly connected to the lower port of the through hole 22. An extension cylinder 272 is slidably connected to the inner wall of the fixed cylinder 271. A fixed ring 273 is fixedly connected to the side wall of the extension cylinder 272 outside the fixed cylinder 271. A drive cylinder 274 is movably sleeved on the side wall of the fixed ring 273. The inner wall of the upper end of the drive cylinder 274 is threadedly connected to the fixed cylinder 271. A sealing component 28 is provided on the inner wall of the upper end of the fixed cylinder 271.

[0041] Specifically, the height difference of the different depths of the cell tissue to be extracted is first determined. By rotating the drive cylinder 274, the threaded connection between it and the fixed cylinder 271 is used to drive the extension cylinder 272 to slide along the inner wall of the fixed cylinder 271, thereby adjusting the extension distance of the extension cylinder 272 to adapt to different extraction depths. Subsequently, the pressure plate 24 squeezes the air bag 25, so that the gas in the air bag 25 enters the fixed cylinder 271 and the extension cylinder 272 of the extraction component 27 through the air hole 251 and the through hole 22 in sequence, and is finally discharged, completing the exhaust preparation before extraction. When the extraction component 27 is inserted into the appropriate position in the separation box 4, the pressure plate 24 is driven in the opposite direction to make the air bag 25 expand and generate negative pressure. Under the action of negative pressure, cell tissues at different depths are drawn in through the corresponding extension cylinder 272 and fixed cylinder 271 respectively. At the same time, the sealing component 28 is used to prevent tissue fluid from entering the air bag 25, so as to achieve simultaneous extraction of cell tissues at different depths.

[0042] The sealing assembly 28 includes a mounting ring 281 fixedly connected to the inner wall of the fixed cylinder 271. Multiple mounting posts 282 are evenly fixedly connected to the lower end of the mounting ring 281, and sealing rings 283 are slidably connected to the side walls of the mounting posts 282.

[0043] Specifically, during device operation, when the airbag 25 is compressed, the sealing ring 283 naturally droops due to its own gravity, and its lower surface maintains a certain distance from the lower surface of the mounting ring 281, without forming a tight fit. This structural relationship allows a smooth airflow channel to be formed inside the fixed cylinder 271, and the compressed air inside the airbag 25 can flow smoothly through this channel through the fixed cylinder 271 and the extended cylinder 272, and finally be discharged outside the device, thus preparing for the subsequent cell tissue extraction stage by venting.

[0044] When the reverse-drive pressure plate 24 causes the airbag 25 to gradually expand under its own elasticity, a negative pressure suction force is generated inside the device. Under this suction force, cells and tissues at different depths in the separation box 4 are sucked into the extension tube 272 and move upward along the extension tube 272. As the cells and tissues are continuously sucked in, when the cells and tissues flow to contact the lower surface of the sealing ring 283, the cells and tissues will generate an upward buoyancy force on the sealing ring 283. Driven by the buoyancy, the sealing ring 283 will slide upward along the side wall of the mounting column 282 until its upper end face is in close contact with the lower surface of the mounting ring 281. At this time, the sealing ring 283 will completely block the channel inside the fixed tube 271, thereby effectively preventing the extracted cell and tissue fluid from flowing further upward into the airbag 25. This ensures the effective collection of cells and tissues and prevents the airbag 25 from being contaminated, ensuring the stability of the device operation and the reliability of the extraction process.

[0045] The drive assembly 26 includes a third mounting plate 261 fixedly connected to the upper end of the mounting base 23. A rotary drive component 262 is fixedly connected to the upper end of the third mounting plate 261. A first gear 263 is fixedly connected to the output end of the rotary drive component 262. A second gear 264 is meshed with the side wall of the first gear 263. A threaded post 265 is fixedly connected to the lower end of the second gear 264. A drive frame 266 is threadedly connected to the side wall of the threaded post 265. The lower end of the drive frame 266 is fixedly connected to the pressure plate 24. The nominal size of the first gear 263 is smaller than the nominal size of the second gear 264. In one embodiment of the present invention, the rotary drive component 262 is a stepper motor, model 57BYG250D, manufactured by Shenzhen Yankong Automation Technology Co., Ltd.

[0046] Specifically, the third mounting plate 261, fixed to the upper end of the mounting base 23, provides stable support for the entire drive assembly 26. The rotating drive component 262 at its upper end serves as a power source. After startup, the output end drives the first gear 263 to rotate. Since the first gear 263 meshes with the side wall of the second gear 264, the rotation of the first gear 263 will drive the second gear 264 to rotate synchronously, thereby driving the threaded column 265 fixedly connected to the lower end of the second gear 264 to rotate together. The side wall of the threaded column 265 is threadedly connected to the drive frame 266. Under the action of threaded transmission, the rotational motion of the threaded column 265 is converted into the linear motion of the drive frame 266. Since the lower end of the drive frame 266 is fixedly connected to the pressure plate 24, the up and down movement of the drive frame 266 will directly drive the pressure plate 24 to move up and down along the inner wall of the mounting base 23, realizing the squeezing or releasing operation of the airbag 25.

[0047] By using a small gear to drive a large gear, the output speed can be reduced while increasing the torque. This allows the power of the rotary drive component 262 to be transmitted to the threaded column 265 more efficiently, ensuring that the drive frame 266 drives the pressure plate 24 to move up and down more smoothly and powerfully. This guarantees the reliable compression or release operation of the airbag 25. Secondly, the lower speed allows for more precise control of the movement distance of the pressure plate 24, making it easier to accurately adjust the compression or expansion of the airbag 25 according to the extraction requirements. This allows for precise control of the amount of gas discharged and the amount of cell tissue absorbed during the extraction process, improving the accuracy of the extraction operation.

[0048] Furthermore, in order to enhance the stability of the device, such as Figures 4-10 As shown, the following preferred technical solutions are provided.

[0049] Both the inner wall at the lower end of the fixed cylinder 271 and the outer wall at the upper end of the extended cylinder 272 are provided with sealing elements 275. In one embodiment of the present invention, the sealing element 275 is a dynamic sealing ring.

[0050] The upper surface of the sealing ring 283 is provided with a first mounting groove 284 and a second mounting groove 286. The inner wall of the first mounting groove 284 is provided with a sealing plate 285, and the inner wall of the second mounting groove 286 is provided with a sealing ring 287. In one embodiment of the present invention, the sealing plate 285 and the sealing ring 287 are made of low-hardness rubber.

[0051] A gap is left between the outer wall of the sealing ring 283 and the inner wall of the fixing cylinder 271.

[0052] Specifically, the gap between the outer wall of the sealing ring 283 and the inner wall of the fixed cylinder 271 serves as a crucial passageway during device operation. When the airbag 25 is compressed, the gas inside the airbag 25 needs to enter the fixed cylinder 271 through the through hole 22 and eventually be discharged. At this time, the sealing ring 283 is in a natural downward state due to gravity, and the gap between its outer wall and the inner wall of the fixed cylinder 271 forms a smooth airflow channel. The gas can flow through this gap in the fixed cylinder 271, ensuring that the gas inside the airbag 25 can be discharged smoothly.

[0053] The two non-adjacent side walls of the pressure plate 24 are respectively fixedly connected to the first limiting block 241. The inner side wall of the mounting base 23 is provided with the first limiting groove 231. The first limiting block 241 is slidably connected to the inner wall of the first limiting groove 231. When the driving component 26 drives the pressure plate 24 to move up and down along the inner wall of the mounting base 23, the first limiting block 241 will slide along the inner wall of the first limiting groove 231. This can limit the pressure plate 24 from lateral displacement or rotation during the movement, ensuring that the pressure plate 24 always moves stably in the vertical direction. This makes the squeezing or releasing force of the pressure plate 24 on the airbag 25 uniform and stable, ultimately ensuring the stability and reliability of the entire extraction process.

[0054] The fourth mounting plate 33 has a second limiting groove 34 on its side wall. A second limiting block 35 is slidably connected to the inner wall of the second limiting groove 34. The second limiting block 35 is fixedly connected to the side wall of the mounting frame 31 to increase the movement stability of the mounting frame 31.

[0055] The fourth mounting plate 33, fixed to the upper surface of the base 1, has a second limiting groove 34 pre-cut in its side wall that extends vertically, providing a guide path for the movement of the mounting frame 31. The second limiting blocks 35, which are fixedly connected to the non-adjacent side walls of the mounting frame 31, are embedded in the inner wall of the second limiting groove 34, forming a tight sliding fit. When the linear drive 3 drives the mounting frame 31 to move up and down, the second limiting blocks 35 will slide synchronously along the inner wall of the second limiting groove 34 with the mounting frame 31. During this process, the groove wall of the second limiting groove 34 forms a lateral constraint on the second limiting blocks 35, effectively limiting the left and right swaying or horizontal deviation that may occur during the movement of the mounting frame 31, ensuring that the mounting frame 31 always moves smoothly in the vertical direction.

[0056] A liquid dispensing hopper 12 is provided on the upper side wall of the vertical plate 11. A fastening plate 13 is fixedly connected to the side wall of the liquid dispensing hopper 12. One end of the fastening plate 13 is fixedly connected to the vertical plate 11. A drain pipe 14 is provided at the lower end of the liquid dispensing hopper 12. A first mounting plate 15 is fixedly connected to the side wall of the middle part of the vertical plate 11. A heating block 16 is provided on the upper end of the first mounting plate 15. A drain box 17 is provided on the upper end of the heating block 16. The lower end of the drain pipe 14 is located in the drain box 17. An outlet pipe 18 is provided on the side wall of the lower end of the drain box 17. In one embodiment of the present invention, the heating block 16 is a ceramic heating block 16, model KHR-200, manufactured by Hangzhou Kaihua Electronics Co., Ltd.

[0057] A linear drive component 3 is provided on the upper end of the base 1. The output end of the linear drive component 3 is fixedly connected to a mounting frame 31. A rotary drive component 32 is fixedly connected to the inner wall of the mounting frame 31. The output end of the rotary drive component 32 passes through the upper surface of the mounting frame 31. A separation box 4 is fixedly connected to the output end of the rotary drive component 32. A fourth mounting plate 33 is provided on each of the two non-adjacent side walls of the mounting frame 31. The lower end of the fourth mounting plate 33 is fixedly connected to the upper surface of the base 1. In one embodiment of the present invention, the rotary drive component 32 is a stepper motor, model 86BYG350C, manufactured by Changzhou Leisai Intelligent Control Co., Ltd., and the linear drive component 3 is an electric push rod, model DT50, manufactured by Shanghai Linake Transmission Technology Co., Ltd.

[0058] Specifically, when extracting microglial cell tissue samples, the operator first pours different types of solutions into the mixing hopper 12 at the top of the vertical plate 11. Using the scale marked on the mixing hopper 12, the ratio between the solutions is precisely controlled to ensure that the concentration of the mixture meets the requirements for cell extraction. After the ratio is completed, the solutions flow through the drain pipe 14 at the bottom of the mixing hopper 12 into the drain box 17 located above the first mounting plate 15. At this time, the heating block 16 at the top of the first mounting plate 15 starts to work. By heating the drain box 17, the molecular movement of the solutions in the box is accelerated, which promotes the rapid and thorough mixing of different components of the solutions to form a uniform cell lysis solution.

[0059] The mixed cell lysis buffer flows into the separation chamber 4 through the outlet pipe 18 on the lower side wall of the leakage tank 17. After a preset reaction time, the lysis buffer completes the lysis treatment of the cell tissue in the separation chamber 4. Then, the rotation drive component 32 on the inner wall of the mounting frame 31 is activated, and its output shaft drives the separation chamber 4 to rotate at high speed. Centrifugal force is used to separate the components of different densities in the cell tissue, preparing for subsequent layered extraction.

[0060] After separation, the linear drive unit 3 at the upper end of the base 1 is activated, and its output end pushes the mounting frame 31 and the separation box 4 to move upward. During this process, the second limiting groove 34 on the fourth mounting plate 33 cooperates with the second limiting block 35 on the side wall of the mounting frame 31 to ensure that the separation box 4 rises steadily until the extraction component 27 at the lower end of the second mounting plate 21 is inserted into the target depth position inside the separation box 4.

[0061] At this time, the drive component 26 at the upper end of the reverse drive mounting base 23 causes the pressure plate 24 to move upward. The originally squeezed airbag 25 expands under its own elasticity, generating negative pressure inside. Under the adsorption effect of negative pressure, cell tissues at different depths in the separation box 4 are sucked into the extraction component 27 through the corresponding extended tube 272 and fixed tube 271. At the same time, the sealing component 28 in the fixed tube 271 works synchronously. When the cell tissue fluid rises to the sealing ring 283, it pushes the sealing ring 283 to move upward and fit with the mounting ring 281, effectively blocking the passage and preventing tissue fluid from entering the airbag 25 and causing contamination, thereby achieving synchronous and pure extraction of cell tissues at different depths.

[0062] After the cell tissue fluid extraction is completed, the reverse drive linear drive 3 is used to lower and reset the separation box 4. The operator places the collection cup directly below each extension tube 272, and then drives the pressure plate 24 to move downward through the drive component 26, squeezing the air bag 25 to smoothly discharge the cell tissue fluid collected in the extraction component 27 into the collection cup through the extension tube 272, thus completing the entire extraction process.

[0063] It should be noted that, in this document, relational terms such as "first" and "second" are used only to distinguish one entity or operation from another, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Furthermore, the terms "comprising," "including," or any other variations thereof are intended to cover non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements includes not only those elements but also other elements not expressly listed, or elements inherent to such process, method, article, or apparatus.

[0064] Although embodiments of the invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made to these embodiments without departing from the principles and spirit of the invention, the scope of which is defined by the appended claims and their equivalents.

Claims

1. A sample extraction device for microglial tissue, comprising a base (1), characterized in that: A vertical plate (11) is fixedly connected to the side wall of the base (1), and an installation component (2) is provided on the side wall of the middle part of the vertical plate (11). The mounting assembly (2) includes a second mounting plate (21) fixedly connected to the side wall of the vertical plate (11). The side wall of the middle part of the second mounting plate (21) is evenly provided with a plurality of through holes (22). The upper end of the second mounting plate (21) is fixedly connected with two mounting seats (23). The inner wall of the mounting seat (23) is movably connected with a pressure plate (24). The lower end of the pressure plate (24) is fixedly connected with an airbag (25). The lower end of the airbag (25) is evenly provided with a plurality of air holes (251). The lower end of the airbag (25) is fixedly connected to the second mounting plate (21). The air holes (251) are located directly above the through holes (22). The upper end of the mounting seat (23) is provided with a driving assembly (26). The lower end of the middle part of the second mounting plate (21) is provided with an extraction assembly (27). The extraction component (27) includes a fixed cylinder (271) fixedly connected to the lower port of the through hole (22). An extension cylinder (272) is slidably connected to the inner wall of the fixed cylinder (271). A fixing ring (273) is fixedly connected to the side wall of the extension cylinder (272) outside the fixed cylinder (271). A driving cylinder (274) is movably sleeved on the side wall of the fixing ring (273). The inner wall of the upper end of the driving cylinder (274) is threadedly connected to the fixed cylinder (271). A sealing component (28) is provided on the inner wall of the upper end of the fixed cylinder (271).

2. The sample extraction device for microglial tissue according to claim 1, characterized in that: The sealing assembly (28) includes a mounting ring (281) fixedly connected to the inner wall of the fixed cylinder (271), and a plurality of mounting posts (282) are evenly fixedly connected to the lower end of the mounting ring (281), and a sealing ring (283) is slidably connected to the side wall of the mounting post (282).

3. The sample extraction device for microglial tissue according to claim 1, characterized in that: The drive assembly (26) includes a third mounting plate (261) fixedly connected to the upper end of the mounting base (23). A rotary drive component (262) is fixedly connected to the upper end of the third mounting plate (261). A first gear (263) is fixedly connected to the output end of the rotary drive component (262). A second gear (264) is meshed with the side wall of the first gear (263). A threaded column (265) is fixedly connected to the lower end of the second gear (264). A drive frame (266) is threadedly connected to the side wall of the threaded column (265). The lower end of the drive frame (266) is fixedly connected to the pressure plate (24).

4. The sample extraction device for microglial tissue according to claim 1, characterized in that: The inner wall at the lower end of the fixed cylinder (271) and the outer wall at the upper end of the extended cylinder (272) are both provided with sealing elements (275).

5. The sample extraction device for microglial tissue according to claim 2, characterized in that: The sealing ring (283) has a first mounting groove (284) and a second mounting groove (286) on its upper surface. The inner wall of the first mounting groove (284) is provided with a sealing plate (285), and the inner wall of the second mounting groove (286) is provided with a sealing ring (287).

6. The sample extraction device for microglial tissue according to claim 2, characterized in that: A gap is left between the outer wall of the sealing ring (283) and the inner wall of the fixing cylinder (271).

7. The sample extraction device for microglial tissue according to claim 1, characterized in that: The pressure plate (24) has a first limiting block (241) fixedly connected to the two non-adjacent side walls at both ends. The mounting base (23) has a first limiting groove (231) on its inner side wall. The first limiting block (241) is slidably connected to the inner wall of the first limiting groove (231).

8. The sample extraction device for microglial tissue according to claim 1, characterized in that: A liquid dispensing hopper (12) is provided on the upper side wall of the vertical plate (11). A fastening plate (13) is fixedly connected to the side wall of the liquid dispensing hopper (12). One end of the fastening plate (13) is fixedly connected to the vertical plate (11). A leakage pipe (14) is provided at the lower end of the liquid dispensing hopper (12). A first mounting plate (15) is fixedly connected to the side wall of the middle part of the vertical plate (11). A heating block (16) is provided at the upper end of the first mounting plate (15). A leakage box (17) is provided at the upper end of the heating block (16). The lower end of the leakage pipe (14) is located inside the leakage box (17). An outlet pipe (18) is provided on the side wall at the lower end of the leakage box (17).

9. The sample extraction device for microglial tissue according to claim 1, characterized in that: A linear drive component (3) is provided on the upper end of the base (1). The output end of the linear drive component (3) is fixedly connected to a mounting frame (31). A second rotary drive component (32) is fixedly connected to the inner wall of the mounting frame (31). The output end of the second rotary drive component (32) penetrates the upper surface of the mounting frame (31). A separation box (4) is fixedly connected to the output end of the second rotary drive component (32). A fourth mounting plate (33) is provided on each of the two non-adjacent side walls of the mounting frame (31). The lower end of the fourth mounting plate (33) is fixedly connected to the upper surface of the base (1).

10. A sample extraction device for microglial tissue according to claim 9, characterized in that: The fourth mounting plate (33) has a second limiting groove (34) on its side wall. A second limiting block (35) is slidably connected to the inner wall of the second limiting groove (34). The second limiting block (35) is fixedly connected to the side wall of the mounting frame (31).

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