Application of maize nitrogen efficient related gene Zmltpg14-zmnpf5.14

By editing the nitrogen-efficient genes ZmNPF5.14 and ZmLTPg14 in maize, CRISPR/Cas9 technology was used to alter the low nitrogen stress resistance of maize, solving the problem of unclear function of maize nitrate transporter proteins and improving the growth performance of maize under low nitrogen conditions.

CN122357598APending Publication Date: 2026-07-10CHINA AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-01-09
Publication Date
2026-07-10

AI Technical Summary

Technical Problem

The function of nitrate transport proteins in maize is unclear, resulting in low nitrogen utilization efficiency in maize, and existing technologies are insufficient to improve maize's resistance to low nitrogen stress.

Method used

By knocking out or overexpressing the nitrogen-efficient genes ZmNPF5.14 and ZmLTPg14, maize genes were edited using CRISPR/Cas9 technology to alter their resistance to low nitrogen stress and enhance or reduce maize's sensitivity to low nitrogen.

Benefits of technology

This study aimed to enhance or reduce maize's resistance to low nitrogen stress, and to improve maize's growth performance under low nitrogen conditions by cultivating low-nitrogen-sensitive or resistant maize varieties.

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Abstract

This invention relates to the application of a nitrogen-efficient gene in maize, ZmLTPg14-NPF5.14. The nitrogen-efficient gene ZmNPF5.14 described in this invention promotes maize resistance to low nitrogen stress. Knocking out this gene reduces maize's resistance to low nitrogen stress. Mutant maize plants with this gene knockout exhibit more yellowing leaves under low nitrogen conditions compared to control plants, showing a more sensitive phenotype. ZmLTPg14 reduces maize's resistance to low nitrogen stress; mutant maize plants with this gene knockout are less sensitive to low nitrogen than control plants. Correspondingly, plants overexpressing ZmLTPg14 are more sensitive to low nitrogen. Furthermore, double mutants with both ZmLTPg14 and ZmNPF5.14 knockouts exhibit the same phenotype under low nitrogen conditions as those with ZmNPF5.14. The mutant of ZmLTPg14 can be used to cultivate maize varieties that are not sensitive to low nitrogen. The mutant plants of ZmNPF5.14, the overexpression plants of ZmLTPg14, and the double mutants of the two genes ZmLTPg14 and ZmNPF5.14 can be used to cultivate maize varieties that are sensitive to low nitrogen.
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Citation Information

Patent Citations

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