Coumarin-based glycosidase substrate compound and synthesis method and application thereof
By designing coumarin-based glycosidase substrate compounds, the problems of poor stability and low synthesis rate of existing chromogenic substrates have been solved, achieving efficient and low-cost chromogenic detection, which is suitable for microbial screening and enzyme activity analysis.
Patent Information
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- JIANGXI SCI & TECH NORMAL UNIV
- Filing Date
- 2026-03-16
- Publication Date
- 2026-07-17
AI Technical Summary
Existing aescin enzyme substrates have poor stability, and S-gal® has a low overall synthesis rate, is expensive, and requires a long cultivation time, which limits its widespread application.
A coumarin-based glycosidase substrate compound with the structure 3,4-cyclohexenyl-7-hydroxy-6-β-D-galactopyranoside coumarin was designed. The structure was well preserved after autoclaving, and the Michaelis constant Km for the reaction with β-galactosidase was low. The synthetic route was simple and suitable for colorimetric detection in solid culture media or gel systems.
It improves the stability and synthesis yield of chromogenic products, shortens the culture time, and reduces production costs. It is suitable for microbial screening and enzyme activity localization analysis, and is applicable to the detection of β-galactosidase and similar glycosidases.
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Abstract
Citation Information
Patent Citations
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US6008008A