Phytosteryl glycoside ester compound and method for preparing the same

Phytosterol glycoside esters were prepared by esterification and silica gel chromatography, solving the problems of water solubility and stability of phytosterols and realizing an efficient and simple preparation method, which expands their application in water-based foods, beverages and cosmetics.

CN122404458APending Publication Date: 2026-07-17NORTHEAST AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NORTHEAST AGRICULTURAL UNIVERSITY
Filing Date
2026-04-14
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

Phytosterols in existing technologies have poor water solubility and stability, and their synthesis methods are cumbersome and their stereoselectivity is difficult to control, making large-scale production difficult.

Method used

Phytosterol glycoside esters, including stigmasterol, β-sitosterol, campesterol, etc., were prepared by esterification reaction of phytosterols and gluconic acid, using cholesterol esterase as a catalyst and silica gel column purification, to prepare esterification products of gluconic acid, lactobionic acid, maltodextrin, etc.

Benefits of technology

This study provides a new class of compounds with well-defined structures, good amphiphilicity and water solubility, significantly improving water solubility to 1-5%, exhibiting unique advantages in interfacial activity and cell membrane permeability, and using a simple and efficient preparation method suitable for large-scale production.

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Abstract

本发明适用于生物化工与食品科学技术领域,涉及一种植物甾醇糖苷酯化合物及其制备方法,所述植物甾醇糖苷酯由豆甾醇、β‑谷甾醇、菜油甾醇或菜籽甾醇中的一种,与葡萄糖酸、乳糖酸、麦芽糖酸或半乳糖二酸中的一种,通过酯化反应合成。其制备方法为:以植物甾醇和糖酸为原料,在含胆固醇酯酶、柠檬酸钠及聚乙二醇800的水相和含植物甾醇的正己烷相构成的两相反应体系中,于28‑42℃下进行酶催化酯化反应;反应液经离心分离、减压蒸馏及硅胶柱层析梯度洗脱纯化,制得高纯度产品,纯度达92‑97%。本发明方法路线简短、条件温和、选择性好;所得产品水溶性显著改善,可作为功能性成分用于食品、药品及化妆品领域。
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