Sti-related virus detection primer group, kit and application

By designing a primer set for STI-related virus detection and using multiplex PCR technology, we were able to simultaneously detect multiple STI viruses from a single sample. This solved the problems of low detection efficiency and lack of co-infection information in existing technologies, and enabled efficient and convenient virus infection detection and identification.

CN122405904APending Publication Date: 2026-07-17WUHAN XINO MEDICAL LABORATORY CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
WUHAN XINO MEDICAL LABORATORY CO LTD
Filing Date
2026-05-25
Publication Date
2026-07-17

AI Technical Summary

Technical Problem

In existing technologies, the detection efficiency of STI-related viruses is low and it is impossible to detect co-infection status of multiple viruses, which requires patients to have multiple samples and tests, which is time-consuming, costly, and lacks co-infection information.

Method used

We designed a primer set for STI-related virus detection, including specific primer sets for detecting high-risk and low-risk HPV and non-HPV sexually transmitted viruses, and combined them with internal control primers to achieve simultaneous detection of multiple viruses through multiplex PCR and sequencing library construction.

Benefits of technology

It enables efficient and convenient detection of 19 key STI viruses from a single sample, including 14 high-risk HPV types, 2 low-risk HPV types, and 3 non-HPV sexually transmitted viruses. It supports the identification of the cause of condyloma acuminata, the differential diagnosis of genital herpes, and the early warning of co-infection risk. The detection accuracy is ≥98%, and the whole process takes less than 24 hours.

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Abstract

本发明提出了STI相关病毒检测引物组、试剂盒及应用,包括用于检测HPV病毒的第一引物组,及用于检测非HPV性传播病毒的第二引物组;所述第一引物组包括如SEQ ID NO:1‑32所示的核苷酸序列,所述第二引物组包括如SEQ ID NO:33‑38所示的核苷酸序列;所述检测引物组能够检测19种关键STI病毒,涵盖高危 / 低危HPV及HSV‑2、CMV、EBV,能够从单一样本中实现14种高危型HPV、2种低危型HPV及3种非HPV性传播病毒病毒的高特异性、高均衡性同步检测,支持尖锐湿疣病因鉴定、生殖器疱疹鉴别诊断、共感染风险预警,为性相关病毒感染临床检测及辅助诊断提供高效、便捷的选择。
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