Preparation and application of a traditional Chinese medicine exosome and traditional Chinese medicine extract mixed hair restorer

Abstract

The application provides a preparation and application of a traditional Chinese medicine exosome and traditional Chinese medicine extract mixed hair growth agent, and relates to the technical field of hair loss prevention and hair growth product processing. The traditional Chinese medicine exosome and traditional Chinese medicine extract mixed hair growth agent is mainly prepared from traditional Chinese medicine exosomes prepared from Radix Polygoni Multiflori, Dendrobium, and Winter Redwood Leaves and traditional Chinese medicine extracts prepared from Ligustrum Lucidum Fruit, Ginger, Radix Polygoni Multiflori, Dendrobium, Winter Redwood Leaves, Honeysuckle, Golden Silk Chrysanthemum, Water Moss, Peach Gum, and Pine Needle, and mixed with disodium isothionate, ethanol, and propylene glycol. The application overcomes the defects of the prior art, and can effectively promote scalp blood circulation, promote hair growth, prevent hair follicle atrophy, and achieve the purpose of preventing hair loss and promoting hair growth through extraction and addition of traditional Chinese medicine exosomes and main chemical components.

Description

Technical Field

[0001] This invention relates to the field of processing technology for hair loss prevention and hair growth products, specifically to the preparation and application of a hair growth agent made from a mixture of Chinese herbal exosomes and Chinese herbal extracts. Background Technology

[0002] Hair loss refers to the phenomenon of hair falling out. Generally, losing about 50-75 hairs a day is considered normal physiological metabolism. However, due to the fast pace of social development, increased stress, and irregular lifestyles, excessive hair loss can easily occur. Along with excessive hair loss, hair growth slows down and hair roots become thinner, which can seriously reduce a person's appearance and aesthetics, thus easily affecting the mental health of people with hair loss.

[0003] Currently, the main treatments for hair loss include surgical treatment and medication. Surgical treatment mainly involves hair transplantation, which is fast-acting, but transplanted hair follicles may still shrink. Furthermore, surgical treatment is expensive and not universally applicable to the general population experiencing hair loss. Medication treatment is generally divided into topical ointments and oral medications. Currently, topical minoxidil ointment is clinically effective in treating hair loss. However, minoxidil has a certain degree of dependence and requires long-term use. Hair loss problems will rebound rapidly after discontinuation.

[0004] Traditional Chinese medicine has been studied for a long time in the treatment of hair loss. Traditional prescriptions such as Bazhen Decoction, Tongqiao Huoxue Decoction, and Qibao Meiran Pill have certain effects in preventing and stopping hair loss. However, these medicines need to be taken orally and are not convenient to take for a long time. With the development of technology, adding Chinese medicine ingredients to shampoos and lotions for external use is also a more effective way to prevent hair loss. For example, using extracts of Chinese herbs such as Polygonum multiflorum and Ligustrum lucidum with anti-hair loss effects for shampooing or scalp application can alleviate hair loss.

[0005] Exosomes are a class of vesicles derived from the inner membrane, with a particle size of 30-200 nm. They consist of a lipid bilayer and contain proteins, deoxyribonucleotides, and ribonucleotides, and play a role in intercellular communication. Exosomes extracted from traditional Chinese medicine are called TCM exosomes. TCM exosomes can effectively penetrate the skin barrier and act on the affected area, achieving precise and effective treatment. Therefore, TCM exosomes can be used to prepare topical hair loss treatments. Further research focuses on optimizing drug ratios and selection to enhance treatment efficacy. Summary of the Invention

[0006] To address the shortcomings of existing technologies, this invention provides a preparation and application of a hair-strengthening agent made from a mixture of Chinese herbal exosomes and Chinese herbal extracts. This agent can effectively promote scalp blood circulation, promote hair growth, prevent hair follicle atrophy, and achieve the purpose of preventing hair loss and strengthening hair.

[0007] To achieve the above objectives, the technical solution of the present invention is implemented through the following technical solution:

[0008] A hair-strengthening agent prepared by mixing traditional Chinese medicine exosomes and traditional Chinese medicine extracts, wherein the hair-strengthening agent is prepared by mixing the following raw materials in parts by weight: 8-12 parts of traditional Chinese medicine exosomes, 10-12 parts of compound traditional Chinese medicine extract, 0.1-0.3 parts of disodium isodesate, 10-20 parts of ethanol, and 10-12 parts of propylene glycol; wherein the traditional Chinese medicine exosomes are prepared by mixing Polygonum multiflorum, Dendrobium nobile, and Morus alba leaves in a mass ratio of 4-6:3-5:1-3; wherein the compound traditional Chinese medicine extract includes extract A, extract B, and extract C. Extract A is obtained by mixing equal masses of extract B and extract C. Extract A is obtained by high-temperature and high-pressure water extraction after mixing privet fruit, ginger, fleeceflower root, dendrobium, and mulberry leaf in a mass ratio of 2-4:0.5-1.5:4-6:3-5:1-3. Extract B is obtained by grinding honeysuckle, chrysanthemum, and Spirogyra in a mass ratio of 1-3:0.5-1.5:0.5-1.5 into a slurry and then extracting with alcohol. Extract C is obtained by mixing equal masses of peach gum and pine needles, followed by drying, pulverizing, fermentation, steam explosion, and then extracting with alcohol.

[0009] Preferably, the method for preparing the traditional Chinese medicine exosomes includes the following steps:

[0010] S1-1. Mix Polygonum multiflorum, Dendrobium nobile, and mulberry leaves in a certain proportion, add 3 times the volume of deionized water, and let stand at 80℃ for 8-10 hours. Then, extract by ultrasound for 10-15 minutes, filter, add 2 times the volume of deionized water to the filter residue and continue to extract by ultrasound for 10-15 minutes, filter again, combine the two filtrates, and vacuum concentrate to 20%-40% of the original volume to obtain a preliminary concentrate for later use.

[0011] S1-2. Centrifuge the above preliminary concentrated solution at a centrifugal force of 2000-6000g for 80 minutes. Then take the supernatant and centrifuge it at a centrifugal force of 10000-15000g for 90 minutes. Then take the supernatant and centrifuge it at a centrifugal force of 80000-120000g for 90 minutes. Take the precipitate, resuspend it in deionized water, and take the lower layer to obtain the Chinese medicine exosomes.

[0012] Preferably, the preparation method of extract A includes the following steps:

[0013] S2-1. Mix privet fruit, ginger, fleeceflower root, dendrobium, and mulberry leaf in a certain proportion, add 5 times the volume of deionized water, pressurize to 6-8 MPa, heat to 80-90℃ and let stand for 30-40 minutes, then filter while hot. Add 3 times the volume of deionized water to the filter residue, pressurize to 6-8 MPa, heat to 80-90℃ and let stand for 30-40 minutes, continue filtering, combine the two filtrates, and obtain a mixed solution for later use.

[0014] S2-2. Vacuum concentrate the above mixture to 20%-40% of its original volume to obtain extract A.

[0015] Preferably, the preparation method of the extract B includes the following steps:

[0016] S3-1. Mix honeysuckle, golden thread chrysanthemum and water spirulina in proportion, add 1 volume of deionized water and grind into a slurry. Then add ethanol solution to adjust the alcohol content of the slurry to 60-70%. Extract at a constant temperature of 50℃ for 1-2 hours, then filter. Add 2 volumes of 60-70% ethanol solution to the filter residue and extract at a constant temperature of 50℃ for 1-2 hours. Filter again and combine the two filtrates to obtain a mixed filtrate for later use.

[0017] S3-2. Vacuum the above mixed filtrate and reflux it with ethanol to obtain extract B.

[0018] Preferably, the preparation method of the extract C includes the following steps:

[0019] S4-1. Mix peach gum and pine needles in a certain proportion, dry them until the moisture content is ≤10%, then crush them to obtain a mixed powder for later use.

[0020] S4-2. After adding the above mixed powder to 1 volume of deionized water and stirring evenly, inoculate with lactic acid bacteria and yeast and ferment for 1-2 days. Then, perform high-temperature steam explosion treatment to obtain steam explosion material for later use.

[0021] S4-3. Add the above-mentioned gas explosion material to ethanol, adjust the ethanol content to 60-70%, then let it stand for 8-10 hours, then filter it. Add 2 times the volume of 60-70% ethanol solution to the filter residue, let it stand for another 2-4 hours, then filter it again. Combine the two filtrates to obtain a comprehensive filtrate for later use.

[0022] S4-4. Vacuum the above combined filtrate and reflux it with ethanol to obtain extract C.

[0023] Preferably, the drying temperature in step S4-1 is 40-50℃, and the mixed powder passes through an 80-mesh sieve.

[0024] Preferably, in step S4-2, the inoculation amounts of lactic acid bacteria and yeast are 2% and 3% of the total mass of the mixed powder, respectively.

[0025] Preferably, in step S4-2, the pressure of the steam explosion treatment is 2 MPa, the temperature is 121°C, and the time of the steam explosion treatment is 2-3 min.

[0026] Preferably, the temperature for the static treatment in step S4-3 is 40-50℃.

[0027] Furthermore, the hair growth agent, which is a mixture of Chinese herbal exosomes and Chinese herbal extracts, is used in the preparation of hair growth, hair nourishment, and hair care products.

[0028] This invention provides a preparation and application of a hair-strengthening agent made from a mixture of traditional Chinese medicine exosomes and extracts, which has the following advantages compared to existing technologies:

[0029] This invention uses a mixture of Polygonum multiflorum, Dendrobium nobile, and mulberry leaves to prepare herbal exosomes. It also extracts the main components of Ligustrum lucidum, ginger, Polygonum multiflorum, Dendrobium nobile, mulberry leaves, honeysuckle, Chrysanthemum indicum, Spirogyra salsa, peach gum, and pine needles through different methods. These components are then mixed with disodium isodesate, ethanol, and propylene glycol to prepare a topical anti-hair loss liniment. Polygonum multiflorum and other traditional Chinese medicines have excellent blood-activating and hair-nourishing effects. Furthermore, the use of Spirogyra salsa and peach gum in this invention further promotes strong hair roots, improves hair follicle health, and prevents hair loss. Detailed Implementation

[0030] To make the objectives, technical solutions, and advantages of the embodiments of the present invention clearer, the technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0031] Example 1:

[0032] I. Preparation of exosomes from traditional Chinese medicine:

[0033] 1. Exosome A:

[0034] (1) Mix Polygonum multiflorum, Dendrobium nobile and mulberry leaves in a mass ratio of 5:4:2 and add 3 times the volume of deionized water. Keep the mixture at 80℃ for 9 hours, extract with ultrasound at 600W for 12 minutes, filter, add 2 times the volume of deionized water to the filter residue and continue to extract with ultrasound at 600W for 12 minutes, filter again, combine the two filtrates, and vacuum concentrate to 30% of the original volume to obtain a preliminary concentrate for later use.

[0035] (2) Centrifuge the above preliminary concentrate at 4000g for 80 minutes, then take the supernatant and centrifuge at 12000g for 90 minutes, then take the supernatant and centrifuge at 100000g for 90 minutes. Take the precipitate, resuspend it in deionized water, and take the lower layer to obtain the Chinese medicine exosome A.

[0036] 2. Exosome B:

[0037] (1) Mix privet fruit, ginger, fleeceflower root, dendrobium, mulberry leaf, honeysuckle, golden thread chrysanthemum, water hyacinth, peach gum, and pine needles in a mass ratio of 3:1:5:4:2:2:1:1:1:1 and add 3 times the volume of deionized water. Keep the mixture at 80℃ for 9 hours, extract with ultrasound at 600W for 12 minutes, filter, add 2 times the volume of deionized water to the filter residue and continue to extract with ultrasound at 600W for 12 minutes, filter again, combine the two filtrates, and vacuum concentrate to 30% of the original volume to obtain a preliminary concentrate for later use.

[0038] (2) Centrifuge the above preliminary concentrate at 4000g for 80 minutes, then take the supernatant and centrifuge at 12000g for 90 minutes, then take the supernatant and centrifuge at 100000g for 90 minutes. Take the precipitate, resuspend it in deionized water, and take the lower layer to obtain the Chinese medicine exosome B.

[0039] II. Preparation of the extract:

[0040] 1. Preparation of extract A:

[0041] (1) Mix privet fruit, ginger, fleeceflower root, dendrobium and winter mulberry leaf in a mass ratio of 3:1:5:4:2, add 5 times the volume of deionized water, pressurize to 7MPa, heat to 85℃ and let stand for 35min, then filter while hot, add 3 times the volume of deionized water to the filter residue, pressurize to 7MPa, heat to 85℃ and let stand for 35min, continue filtering, combine the two filtrates to obtain a mixed liquid for later use.

[0042] (2) The above mixture is vacuum concentrated to 30% of its original volume to obtain extract A.

[0043] 2. Preparation of Extract B:

[0044] (1) Mix honeysuckle, golden chrysanthemum and water spirulina in a mass ratio of 2:1:1 and add 1 volume of deionized water to grind into a slurry. Then add ethanol solution to adjust the alcohol content of the slurry to 65%. Extract at a constant temperature of 50℃ for 1.5h. Filter and add 2 volumes of 65% ethanol solution to the filter residue. Extract at a constant temperature of 50℃ for 1.5h. Filter and combine the two filtrates to obtain a mixed filtrate for later use.

[0045] (2) Vacuum the above mixed filtrate and reflux it with ethanol to obtain extract B.

[0046] 3. Preparation of extract C:

[0047] (1) Mix peach gum and pine needles in equal proportions, dry at 45°C until the moisture content is ≤10%, then pulverize and pass through an 80-mesh sieve to obtain mixed powder for later use;

[0048] (2) After adding the above mixed powder to 1 volume of deionized water and stirring evenly, inoculate 2% of the total mass of the mixed powder with lactic acid bacteria and 3% of the total mass of the mixed powder with yeast and ferment at room temperature for 30 hours. Then, use 121℃ hot steam to perform high-temperature steam explosion treatment for 150 seconds under 2MPa pressure to obtain steam explosion material for later use.

[0049] (3) Add the above-mentioned gas explosion material to ethanol, adjust the ethanol content to 65%, then let it stand at 45°C for 9 hours, then filter it, add 2 times the volume of 65% ethanol solution to the filter residue, continue to let it stand at 45°C for 3 hours, then filter it, combine the two filtrates to obtain the comprehensive filtrate for use.

[0050] (4) Vacuum the above-mentioned filtrate and reflux it with ethanol to obtain extract C.

[0051] 4. Preparation of extract D:

[0052] (1) Mix privet fruit, ginger, fleeceflower root, dendrobium, winter mulberry leaf, honeysuckle, golden thread chrysanthemum, water hyacinth, peach gum, and pine needles in a mass ratio of 3:1:5:4:2:2:1:1:1:1 and add 5 times the volume of deionized water. Pressurize to 7 MPa and heat to 85°C. Let stand for 35 minutes. Then filter while hot. Add 3 times the volume of deionized water to the filter residue, pressurize to 7 MPa, heat to 85°C, and let stand for 35 minutes. Filter again and combine the two filtrates to obtain a mixed solution for later use.

[0053] (2) The above mixture is vacuum concentrated to 30% of its original volume to obtain extract D.

[0054] 5. Preparation of extract E:

[0055] (1) Mix honeysuckle and golden thread chrysanthemum at a mass ratio of 2:1, add 1 volume of deionized water and grind into a slurry. Then add ethanol solution to adjust the alcohol content of the slurry to 65%. Extract at a constant temperature of 50℃ for 1.5h, then filter. Add 2 volumes of 65% ethanol solution to the filter residue and extract at a constant temperature of 50℃ for 1.5h. Filter again and combine the two filtrates to obtain a mixed filtrate for later use.

[0056] (2) Vacuum the above mixed filtrate and reflux it with ethanol to obtain extract E.

[0057] 6. Preparation of extract F:

[0058] (1) Dry the pine needles at 45°C until the moisture content is ≤10%, then crush them and pass them through an 80-mesh sieve to obtain a mixed powder for later use;

[0059] (2) After adding the above mixed powder to 1 volume of deionized water and stirring evenly, inoculate 2% of the total mass of the mixed powder with lactic acid bacteria and 3% of the total mass of the mixed powder with yeast and ferment at room temperature for 30 hours. Then, use 121℃ hot steam to perform high-temperature steam explosion treatment for 150 seconds under 2MPa pressure to obtain steam explosion material for later use.

[0060] (3) Add the above-mentioned gas explosion material to ethanol, adjust the ethanol content to 65%, then let it stand at 45°C for 9 hours, then filter it, add 2 times the volume of 65% ethanol solution to the filter residue, continue to let it stand at 45°C for 3 hours, then filter it, combine the two filtrates to obtain the comprehensive filtrate for use.

[0061] (4) Vacuum the above-mentioned comprehensive filtrate and reflux it with ethanol to obtain extract F.

[0062] III. Preparation of Compound Traditional Chinese Medicine Extracts:

[0063] 1. Compound Chinese herbal extract A: Obtained by mixing equal masses of extract A, extract B, and extract C as described above;

[0064] 2. Compound Chinese herbal extract B: This is obtained by mixing equal masses of extracts A, E, and F.

[0065] 3. Compound Chinese herbal extract C is the same as the above-mentioned extract D.

[0066] Example 2:

[0067] Preparation of hair growth regulator:

[0068] Prepare the materials according to the weight proportions in Table 1 below:

[0069] Table 1

[0070]

[0071]

[0072] After thoroughly mixing the raw materials of the above experimental groups, hair growth promoters for each group were obtained.

[0073] Detection:

[0074] 1. A saline solution was used as the control group. Eighteen male and female C57BL / 6 mice aged 6 weeks were randomly divided into 6 groups, with 3 males and 3 females in each group. On the same day, the back hair of the mice was removed, and the mice were lightly anesthetized with ether. After anesthesia, the back hair of each mouse was removed with wax. The mice were then fed routinely. On the second day of feeding, the hair-regenerating agent of experimental groups 1-5 and the saline solution of the control group were applied to the hair-removed areas of each group of mice (the amount applied was 10 ml / kg of mouse weight). The application was done twice a day, morning and evening, for 10 consecutive days. During the application process, the mice's backs were observed for redness, swelling, damage, or other lesions. On the 10th day, the skin of the hair-removed area on the back of the mice was taken and prepared into pathological sections to observe the hair follicle growth. The specific results are shown in Table 2 below.

[0075] Table 2

[0076]

[0077] As shown in the table above, the hair growth promoter prepared in experimental group 1 has a good effect on promoting hair follicle growth.

[0078] 2. Eight 6-week-old male and female C57BL / 6 mice (4 males and 4 females) were randomly divided into two groups: one group (3 males and 3 females) for the hair regrowth experiment and the other group (1 male and 1 female) for the control group. Hair was removed from 1cm x 1cm areas on both sides of the spine of each mouse. For the hair regrowth experimental group, the left side of each mouse was treated with the hair-growth agent from Experimental Group 1, and the right side with 2% minoxidil solution, at a dosage of 5ml / kg. Both sides were treated once daily at the same time. For the control group, physiological saline was applied to both sides of the hair-removed area at a dosage of 5ml / kg, at the same time as the hair regrowth experimental group.

[0079] Five hairs were randomly plucked from each mouse on its left and right sides at 15, 18, and 21 days. The length of the hairs was measured, and the average length was calculated. The specific results are shown in Table 3 below.

[0080] Table 3

[0081]

[0082] As shown in the table above, the hair growth agent prepared in experimental group 1 can effectively promote hair growth in mice, and its coarse hair growth efficiency is higher than that of 2% minoxidil liniment.

[0083] The above embodiments are only used to illustrate the technical solutions of the present invention, and are not intended to limit it. Although the present invention has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that modifications can still be made to the technical solutions described in the foregoing embodiments, or equivalent substitutions can be made to some of the technical features. Such modifications or substitutions do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. Preparation of a hair-strengthening agent made from a mixture of traditional Chinese medicine exosomes and traditional Chinese medicine extracts, characterized in that, The preparation method of the hair growth regulator is to mix the following raw materials in parts by weight: 8-12 parts of Chinese herbal exosomes, 10-12 parts of compound Chinese herbal extract, 0.1-0.3 parts of disodium isodesate, 10-20 parts of ethanol, and 10-12 parts of propylene glycol. The herbal exosomes mentioned above are exosomes prepared by mixing Polygonum multiflorum, Dendrobium nobile, and Morus alba leaves in a mass ratio of 4-6:3-5:1-3. The compound Chinese herbal extract comprises extract A, extract B, and extract C, obtained by mixing them in equal mass. Extract A is obtained by high-temperature and high-pressure water extraction after mixing privet fruit, ginger, fleeceflower root, dendrobium, and mulberry leaf in a mass ratio of 2-4:0.5-1.5:4-6:3-5:1-3. Extract B is obtained by mixing honeysuckle, chrysanthemum, and Spirogyra in a mass ratio of 1-3:0.5-1.5:0.5-1.5, grinding them into a slurry, and then extracting with alcohol. Extract C is obtained by mixing peach gum and pine needles in equal mass, followed by drying, pulverizing, fermentation, steam explosion, and then extracting with alcohol.

2. The preparation of a hair-strengthening agent composed of a mixture of traditional Chinese medicine exosomes and traditional Chinese medicine extracts according to claim 1, characterized in that, The method for preparing the exosomes of the traditional Chinese medicine includes the following steps: S1-1. Mix Polygonum multiflorum, Dendrobium nobile, and mulberry leaves in a certain proportion, add 3 times the volume of deionized water, and let stand at 80℃ for 8-10 hours. Then, extract by ultrasound for 10-15 minutes, filter, add 2 times the volume of deionized water to the filter residue and continue to extract by ultrasound for 10-15 minutes, filter again, combine the two filtrates, and vacuum concentrate to 20%-40% of the original volume to obtain a preliminary concentrate for later use. S1-2. Centrifuge the above preliminary concentrated solution at a centrifugal force of 2000-6000g for 80 minutes. Then take the supernatant and centrifuge it at a centrifugal force of 10000-15000g for 90 minutes. Then take the supernatant and centrifuge it at a centrifugal force of 80000-120000g for 90 minutes. Take the precipitate, resuspend it in deionized water, and take the lower layer to obtain the Chinese medicine exosomes.

3. The preparation of a hair-strengthening agent composed of a mixture of traditional Chinese medicine exosomes and traditional Chinese medicine extracts according to claim 1, characterized in that, The preparation method of the extract A includes the following steps: S2-1. Mix privet fruit, ginger, fleeceflower root, dendrobium, and mulberry leaf in a certain proportion, add 5 times the volume of deionized water, pressurize to 6-8 MPa, heat to 80-90℃ and let stand for 30-40 minutes, then filter while hot. Add 3 times the volume of deionized water to the filter residue, pressurize to 6-8 MPa, heat to 80-90℃ and let stand for 30-40 minutes, continue filtering, combine the two filtrates, and obtain a mixed solution for later use. S2-2. Vacuum concentrate the above mixture to 20%-40% of its original volume to obtain extract A.

4. The preparation of a hair-strengthening agent composed of a mixture of traditional Chinese medicine exosomes and traditional Chinese medicine extracts according to claim 1, characterized in that, The preparation method of the extract B includes the following steps: S3-1. Mix honeysuckle, golden thread chrysanthemum and water spirulina in proportion, add 1 volume of deionized water and grind into a slurry. Then add ethanol solution to adjust the alcohol content of the slurry to 60-70%. Extract at a constant temperature of 50℃ for 1-2 hours, then filter. Add 2 volumes of 60-70% ethanol solution to the filter residue and extract at a constant temperature of 50℃ for 1-2 hours. Filter again and combine the two filtrates to obtain a mixed filtrate for later use. S3-2. Vacuum the above mixed filtrate and reflux it with ethanol to obtain extract B.

5. The preparation of a hair-strengthening agent composed of a mixture of traditional Chinese medicine exosomes and traditional Chinese medicine extracts according to claim 1, characterized in that, The preparation method of the extract C includes the following steps: S4-1. Mix peach gum and pine needles in a certain proportion, dry them until the moisture content is ≤10%, then crush them to obtain a mixed powder for later use. S4-2. After adding the above mixed powder to 1 volume of deionized water and stirring evenly, inoculate with lactic acid bacteria and yeast and ferment for 1-2 days. Then, perform high-temperature steam explosion treatment to obtain steam explosion material for later use. S4-3. Add the above-mentioned gas explosion material to ethanol, adjust the ethanol content to 60-70%, then let it stand for 8-10 hours, then filter it. Add 2 times the volume of 60-70% ethanol solution to the filter residue, let it stand for another 2-4 hours, then filter it again. Combine the two filtrates to obtain a comprehensive filtrate for later use. S4-4. Vacuum the above combined filtrate and reflux it with ethanol to obtain extract C.

6. The preparation of a hair-strengthening agent composed of a mixture of traditional Chinese medicine exosomes and traditional Chinese medicine extracts according to claim 5, characterized in that: The drying temperature in step S4-1 is 40-50℃, and the mixed powder passes through an 80-mesh sieve.

7. The preparation of a hair-strengthening agent composed of a mixture of traditional Chinese medicine exosomes and traditional Chinese medicine extract according to claim 5, characterized in that: In step S4-2, the inoculation amounts of lactic acid bacteria and yeast are 2% and 3% of the total mass of the mixed powder, respectively.

8. The preparation of a hair-strengthening agent composed of a mixture of traditional Chinese medicine exosomes and traditional Chinese medicine extracts according to claim 5, characterized in that: In step S4-2, the pressure for steam explosion treatment is 2 MPa, the temperature is 121℃, and the treatment time is 2-3 minutes.

9. The preparation of a hair-strengthening agent composed of a mixture of traditional Chinese medicine exosomes and traditional Chinese medicine extracts according to claim 5, characterized in that: The temperature for the static treatment in step S4-3 is 40-50℃.

10. The application of a hair-strengthening agent made from a mixture of traditional Chinese medicine exosomes and traditional Chinese medicine extracts as described in claims 1-9, characterized in that: The hair growth agent is used in the preparation of hair growth, hair nourishment, and hair care products.

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